Temperature control behavior test box

Abstract

The invention relates to the technical field of mouse testing, in particular to a temperature control behavior testing box which comprises a bearing assembly and a temperature control assembly. The supporting assembly comprises an overturning frame rotationally arranged in the box body, a connecting frame integrally formed at the end part of the overturning frame and a rotating plate movably connected with the connecting frame; the test frame and the test rack are detachably arranged in the box body; wherein a camera part is movably arranged on the overturning frame, and a first heating plate is arranged at the opening of the outer wall of the rotating plate. The temperature control behavior test box provided by the invention not only can provide illumination, temperature, humidity and other environments meeting conditions, but also can adjust the position of the camera for recording through the arrangement of the overturning frame, so that the accuracy of the temperature control behavior test box is improved. And standardized acquisition and quantitative analysis of behavioral data are realized. When different tests are carried out, the mouse does not need to be transferred into each special box body, so that a reliable basis is provided for preclinical research, drug safety evaluation and the like.

Description

Technical Field

[0001] The present invention relates to the technical field of mouse testing, in particular to a temperature-controlled behavior testing box. Background Art

[0002] In the field of modern scientific research, mice are one of the most commonly used model animals in biomedical research. Their physiological characteristics, genetic background and behavioral responses are highly similar to those of humans. By precisely controlling the breeding environment and behavioral testing conditions, they can provide stable and reliable experimental models for disease mechanism research, drug development, neurobiology and other fields.

[0003] Different behavioral experiments have specific requirements for environmental conditions and data collection methods. For example, sleep EEG recordings and open-field experiments require a top-down camera to record the overall activity of mice, while forced swimming and tail suspension experiments require a head-on camera to record specific behavioral details of mice. Precise environmental control and targeted image acquisition methods can meet the needs of these experiments, thereby obtaining accurate and reliable experimental data, helping researchers gain a deeper understanding of mouse behavioral patterns, physiological responses, and related disease mechanisms, providing an important reference for biomedical research and drug development.

[0004] To this end, we propose a chamber that meets various mouse testing conditions. Summary of the Invention

[0005] In view of the problems existing in the prior art, the present invention is proposed.

[0006] In order to solve the above technical problems, the present invention provides the following technical solutions: a temperature-controlled behavior test box, comprising: a load-bearing component, comprising a box body; and a supporting component, comprising a flip frame rotatably arranged in the box body, a connecting frame integrally formed at the end of the flip frame, and a rotating plate movably connected to the connecting frame; and a test frame and a test frame detachably arranged inside the box body; wherein, a camera is movably arranged on the flip frame, and a first heating plate is provided at the opening of the outer wall of the rotating plate.

[0007] As a preferred solution of the temperature-controlled behavior test box described in the present invention, the outer wall of the box is provided with several cooling fans, and the inner wall of the box is provided with an insulation board, a box door is provided at the opening of the outer wall of the box, and the outer wall of the box is provided with a mounting plate.

[0008] As a preferred solution of the temperature-controlled behavior test box of the present invention, a movable channel is opened through the outer wall of the flip frame, a slide groove is opened through the inner wall of the movable channel, and a positioning through hole connected to the slide groove is opened through the outer wall of the flip frame.

[0009] As a preferred solution of the temperature-controlled behavior test box of the present invention, the camera component includes a connecting plate and a camera arranged on the connecting plate, and a rotating frame for carrying the camera is arranged in the connecting plate through a rotating shaft.

[0010] As a preferred solution of the temperature-controlled behavior test box described in the present invention, a fill light is provided on the end face of the connecting plate, and side wing plates that slide in cooperation with the slide groove are respectively installed on both side walls of the connecting plate by bolts, and a plug plate movably connected to the positioning through hole is provided inside the side wing plate.

[0011] As a preferred solution of the temperature-controlled behavior test box of the present invention, a control board is movably provided on the outer wall of the side wing plate, and one end of the plug board is connected to the control board.

[0012] As a preferred solution of the temperature-controlled behavior test box of the present invention, it also includes a fixing component, including two clamping plates symmetrically arranged on the end faces of the box body, and the end faces of the clamping plates are provided with slots for fixing the test frame.

[0013] As a preferred solution of the temperature-controlled behavior test box described in the present invention, a fixed frame is installed inside the box body by bolts, and a transmission block is movably provided in the fixed frame, and a second elastic member is provided between the transmission block and the inner wall of the fixed frame.

[0014] As a preferred solution of the temperature-controlled behavior test box described in the present invention, a limiting channel is opened inside the clamping plate, and a first elastic member is provided in the limiting channel. A movable block is also slidably provided in the limiting channel, and the outer wall of the movable block extends to the outside of the clamping plate and is provided with a rotating rod.

[0015] As a preferred solution of the temperature-controlled behavior test box described in the present invention, wherein: a support channel is opened through the outer wall of the rotating rod, the end of the transmission block is movably connected to the support channel, there are two rotating rods, the two rotating rods are cross-shaped, and one end of each rotating rod is respectively connected to the adjacent clamping plate; a positioning screw is installed on the end face of the transmission block through a bearing, and one end of the positioning screw extends to the outside of the fixed frame, and a nut that is threadedly engaged with the positioning screw is embedded in the outer wall of the fixed frame.

[0016] The present invention provides a temperature-controlled behavioral testing chamber that not only provides a satisfactory lighting, temperature, and humidity environment, but also features a tilting rack that allows for adjustment of the recording camera position, enabling standardized collection and quantitative analysis of behavioral data. This eliminates the need to transport mice to separate dedicated chambers for different tests, providing a reliable basis for preclinical research and drug safety evaluation. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] To more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. Those skilled in the art can also derive other drawings based on these drawings without inventive effort. Among them:

[0018] Figure 1 It is a schematic diagram of the overall structure of the present invention.

[0019] Figure 2 It is a schematic diagram of the interior of the box structure in the present invention.

[0020] Figure 3 This is a schematic diagram of the test frame and test stand structure in the present invention.

[0021] Figure 4 Schematic diagram of the supporting component structure in the present invention.

[0022] Figure 5 Schematic diagram of the camera structure in the present invention.

[0023] Figure 6 This is a schematic diagram of the first state of the turnover frame structure in the present invention.

[0024] Figure 7 This is a schematic diagram of the second state of the turnover frame structure in the present invention.

[0025] Figure 8 It is a schematic diagram of the structure of the fixing component in the present invention.

[0026] In the figure: 10, bearing assembly; 11, box body; 111, cooling fan; 112, mounting plate; 113, insulation board; 12, box door; 20, support assembly; 21, flip frame; 211, connecting frame; 212, movable channel; 213, slide; 213-1, positioning hole; 22, rotating plate; 221, first heating plate; 23, camera; 231, connecting plate; 232, camera; 233, rotating frame ; 234, side panel; 235, control panel; 236, plug-in board; 237, fill light; 30, test frame; 31, test stand; 40, fixing assembly; 41, clamping plate; 411, slot; 412, limiting channel; 413, first elastic member; 414, movable block; 42, rotating rod; 421, supporting channel; 43, fixing frame; 431, transmission block; 432, second elastic member; 44, positioning screw. DETAILED DESCRIPTION

[0027] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific embodiments of the present invention are described in detail below with reference to the accompanying drawings.

[0028] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0029] Secondly, the term "one embodiment" or "embodiment" herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in various places throughout this specification does not necessarily refer to the same embodiment, nor does it refer to a separate or selective embodiment that is mutually exclusive of other embodiments.

[0030] Example 1, reference Figures 1 to 8 , which is the first embodiment of the present invention.

[0031] Specifically, the carrier assembly 10 includes a housing 11; a support assembly 20 comprising a flip frame 21 rotatably mounted within the housing 11, a connecting frame 211 integrally formed at the end of the flip frame 21, and a rotating plate 22 movably connected to the connecting frame 211; and a test frame 30 and a test stand 31 detachably mounted within the housing 11. The flip frame 21 is movably mounted with a camera 23, while the rotating plate 22 is provided with a first heating plate 221 at an opening on its outer wall. A second heating plate and a full-spectrum LED light are also provided on the side walls of the housing 11.

[0032] Preferably, the outer wall of the box body 11 is provided with several cooling fans 111, and the inner wall of the box body 11 is provided with an insulation board 113, the outer wall opening of the box body 11 is provided with a box door 12, and the outer wall of the box body 11 is provided with a mounting plate 112. To meet the ventilation of the box body, two cooling fans 111 are provided, and ventilation is performed for 15 minutes per hour.

[0033] A humidifier and a humidity controller are also provided in the box 11 to control the humidity in the box 11 to be maintained within a suitable humidity range (50%-60%) for mouse breeding.

[0034] Based on the experimental design, feeding, and behavioral testing temperature requirements, temperature controllers were set to control the cooling and heating modules to achieve the specified temperature and temperature change pattern. The heating module consisted of two silicone heating plates: the first heating plate 221 and the second heating plate. The temperature of each heating plate was set to 70°C to meet the required heating rate.

[0035] In daily mode:

[0036] 1. Lighting and ventilation settings: Based on the circadian rhythm characteristics of mice (12-hour light-dark cycle), the full-spectrum LED light was set to turn on at 7:00 AM and off at 7:00 PM every day. To ensure the ventilation of the box 11, two cooling fans 111 (one for suction and one for exhaust) were set, and ventilation was carried out for 15 minutes per hour.

[0037] 2. Humidity and temperature settings: Set the humidity controller to 50%-60%; according to the experimental design and feeding temperature requirements, set the temperature controller to control the cooling module and heating module respectively to achieve the specified temperature and temperature change mode, and the two heating plates work.

[0038] 3. Image acquisition: The top camera 23 records from a bird's-eye view.

[0039] Example 2, reference Figures 1 to 8 , which is the second embodiment of the present invention.

[0040] Specifically, a movable channel 212 is formed through the outer wall of the flip frame 21, and a sliding groove 213 is formed on the inner wall of the movable channel 212. A positioning through-hole 213-1 is formed through the outer wall of the flip frame 21 and communicates with the sliding groove 213. The movable channel 212 facilitates the rotation of the rotating plate 22 and prevents movement conflicts between the rotating plate 22 and the flip frame 21.

[0041] The camera 23 includes a connecting plate 231 and a camera 232 disposed on the connecting plate 231. A rotating frame 233 for supporting the camera 232 is provided in the connecting plate 231 via a rotating shaft. The rotating frame 233 can support the camera 233 to flip at a maximum angle of 180°.

[0042] A fill light 237 is installed on the end face of the connecting plate 231. Side panels 234 are bolted to both sides of the connecting plate 231, each slidably engaged with the chute 213. Inside the side panels 234 is an insert plate 236 movably connected to the positioning hole 213-1. The fill light 237 meets the lighting requirements of the camera 232 during video recording. The sliding engagement between the side panels 234 and the chute 213 enables the camera 232 to capture video at different heights. A control panel 235 is movably mounted on the outer wall of the side panels 234, with one end of the insert plate 236 connected to the control panel 235.

[0043] It should be noted that a compression spring is provided at one end of the plug plate 236. When the two control plates 235 move relative to each other, the compression spring is stressed, and when the plug plate 236 is out of contact with the positioning through hole 213-1, the position of the camera element 23 can be moved.

[0044] This test box meets various behavioral tests, including:

[0045] 1. Sleep EEG recording, open field test, cross elevated test, Y maze, three-box social interaction, etc.

[0046] Under this behavior test, see Figure 6 According to the characteristics of the mouse's circadian rhythm (12-hour light-dark cycle), the full-spectrum LED light was set to turn on at 7 a.m. and off at 7 p.m. every day; to ensure the ventilation of the box, two fans (one suction and one exhaust) were set, and ventilation was carried out for 15 minutes per hour; the humidity controller was set to 50%-60%; according to the experimental design and the breeding temperature requirements, the temperature controller was set to control the cooling module and the heating module respectively to achieve the specified temperature and temperature change mode, and the left and right heating plates were working.

[0047] In terms of image acquisition, the camera 232 on the top of the box 11 records from a bird's-eye view.

[0048] 2. Forced swimming, tail suspension test, etc.

[0049] Under this behavior test, see Figure 7 Based on the mouse's circadian rhythm (12-hour light-dark cycle), a full-spectrum LED light was set to on at 7:00 AM and off at 7:00 PM daily. To ensure adequate ventilation, two fans (one for suction and one for exhaust) were installed, providing 15 minutes of ventilation per hour. The humidity controller was set to 50%-60%. Based on the experimental design and the required temperature, temperature controllers were set to control the cooling and heating modules to achieve the specified temperature and temperature change pattern. The heating plates on the top and side of the chamber 11 were activated. For image acquisition, a camera 232 on the side wall of the chamber 11 recorded images at a head-on perspective.

[0050] It should be noted that when the camera 232 needs to record from a top-down perspective, the flip frame 21 can be rotated to the top of the inner cavity of the box 11, and the rotating plate 22 can be flipped so that the first heating plate 221 is located on a side wall of the box 11, thereby meeting the temperature requirement. When the camera 232 needs to record from a level perspective, the flip frame 21 can be flipped vertically, the rotating plate 22 can be flipped horizontally, and the height of the camera 232 can be controlled to meet the level viewing requirement.

[0051] Example 3, reference Figures 1 to 8 , which is the third embodiment of the present invention.

[0052] Specifically, a fixing assembly 40 is also included, including two clamping plates 41 symmetrically arranged on the inner end surface of the box body 11. The end surfaces of the clamping plates 41 are provided with a slot 411 for fixing the test frame 31. The fixing assembly 40 can fix the test frame 30 and the test frame 31.

[0053] A fixing frame 43 is fixed inside the box body 11 by bolts, and a transmission block 431 is movably provided inside the fixing frame 43. A second elastic member 432 is provided between the transmission block 431 and the inner wall of the fixing frame 43. The second elastic member 432 is a compression spring.

[0054] The clamping plate 41 defines a limiting channel 412 within which a first elastic member 413 is disposed. A movable block 414 is also slidably disposed within the limiting channel 412. The outer wall of the movable block 414 extends to the exterior of the clamping plate 41 and is provided with a rotating rod 42. The first elastic member 413 is also a compression spring, and each clamping plate 41 has a slidable movable block 414 disposed within the limiting channel 412.

[0055] A support channel 421 is provided through the outer wall of the rotating rod 42, and the end of the transmission block 431 is movably connected to the support channel 421. There are two rotating rods 42, and the two rotating rods 42 are cross-shaped, and one end of each rotating rod 42 is connected to the adjacent clamping plate 41; a positioning screw 44 is installed on the end face of the transmission block 431 through a bearing, and one end of the positioning screw 44 extends to the outside of the fixed frame 43, and a nut that is threadedly engaged with the positioning screw 44 is embedded in the outer wall of the fixed frame 43.

[0056] In summary, when the test frame 30 needs to be fixed, the test frame 30 can be placed between the two clamping plates 41. By rotating the positioning screw 44, with the cooperation of the nut, the positioning screw 44 gradually moves out of the fixed frame 43, and at the same time, the transmission block 431 will also move synchronously. It should be noted that a bearing is provided between the positioning screw 44 and the transmission block 431. That is, the rotation of the positioning screw 44 does not cause the transmission block 431 to rotate, but only causes the transmission block 431 to move.

[0057] The transmission block 431 pulls the two clamping plates 41 toward each other through the support channel 421 on the rotating rod 42, thereby securing the test frame 30. To secure the test frame 31 for performing a tail suspension test on mice, the test frame 31 is simply snapped into the slot 411 on the upper end of the clamping plate 41.

[0058] Finally, it should be pointed out that the methods and devices described in detail above are merely embodiments, and those skilled in the art can modify these embodiments in different ways without departing from the scope of the present invention.

Claims

1. A temperature-controlled behavior test chamber, characterized in that: include, The carrier assembly (10) includes a box (11); and A supporting assembly (20) includes a turning frame (21) rotatably disposed in the box (11), a connecting frame (211) integrally formed at the end of the turning frame (21), and a rotating plate (22) movably connected to the connecting frame (211); and A test frame (30) and a test stand (31) are detachably arranged inside the box (11); wherein, A camera element (23) is movably provided on the flip frame (21), and a first heating plate (221) is provided at an opening on the outer wall of the rotating plate (22).

2. The temperature-controlled behavior test box according to claim 1, wherein: The outer wall of the box body (11) is provided with a plurality of heat dissipation fans (111), and the inner wall of the box body (11) is provided with a heat preservation plate (113). The outer wall opening of the box body (11) is provided with a box door (12), and the outer wall of the box body (11) is provided with a mounting plate (112).

3. The temperature-controlled behavior test box according to claim 2, wherein: The outer wall of the turnover frame (21) is provided with a movable channel (212), and the inner wall of the movable channel (212) is provided with a sliding groove (213). The outer wall of the turnover frame (21) is provided with a positioning through hole (213-1) communicating with the sliding groove (213).

4. The temperature-controlled behavior test box according to claim 3, wherein: The camera element (23) comprises a connecting plate (231) and a camera (232) arranged on the connecting plate (231); a rotating frame (233) for carrying the camera (232) is arranged in the connecting plate (231) via a rotating shaft.

5. The temperature-controlled behavior test box according to claim 4, wherein: The end surface of the connecting plate (231) is provided with a fill light (237), and the two side walls of the connecting plate (231) are respectively installed with side wing plates (234) that are slidably matched with the sliding groove (213) through bolts, and the side wing plates (234) are provided with plug plates (236) that are movably connected to the positioning through hole (213-1).

6. The temperature-controlled behavior test box according to claim 5, wherein: A control panel (235) is movably provided on the outer wall of the side wing panel (234), and one end of the plug panel (236) is connected to the control panel (235).

7. The temperature-controlled behavior test box according to claim 1, wherein: It also includes a fixing assembly (40) comprising two clamping plates (41) symmetrically arranged on the inner end surface of the box body (11), and the end surfaces of the clamping plates (41) are provided with a clamping slot (411) for fixing the test frame (31).

8. The temperature-controlled behavior test box according to claim 7, wherein: A fixing frame (43) is installed inside the box body (11) by means of bolts, and a transmission block (431) is movably provided inside the fixing frame (43), and a second elastic member (432) is provided between the transmission block (431) and the inner wall of the fixing frame (43).

9. The temperature-controlled behavior test box according to claim 8, wherein: A limiting channel (412) is provided inside the clamping plate (41), and a first elastic member (413) is provided inside the limiting channel (412). A movable block (414) is also slidably provided inside the limiting channel (412), and an outer wall of the movable block (414) extends to the outside of the clamping plate (41) and is provided with a rotating rod (42).

10. The temperature-controlled behavior test box according to claim 9, wherein: A support channel (421) is provided through the outer wall of the rotating rod (42), and the end of the transmission block (431) is movably connected to the support channel (421). There are two rotating rods (42), and the two rotating rods (42) are in a cross shape, and one end of each rotating rod (42) is respectively connected to the adjacent clamping plate (41); a positioning screw (44) is installed on the end face of the transmission block (431) through a bearing, and one end of the positioning screw (44) extends to the outside of the fixed frame (43), and a nut that is threadedly engaged with the positioning screw (44) is embedded in the outer wall of the fixed frame (43).

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