Feed for improving sperm quality after sperm freezing as well as preparation method and application of feed

Abstract

The invention provides a feed for improving sperm quality after sperm freezing as well as a preparation method and application thereof, and belongs to the technical field of animal feeds. The feed for improving the sperm quality of breeding male rabbits and improving the conception rate of female rabbits comprises the following components in parts by weight: 10-20 parts of corn, 50-60 parts of soybean meal, 12-20 parts of fish meal, 5-10 parts of premix and 0.1-0.3 part of vitamin E. The feed improves the sperm motility of fresh sperms, the sperm motility after freezing, the acrosome integrity rate, the plasma membrane integrity rate and the mitochondrial membrane potential of the sperms after the fresh sperms and the frozen sperms.

Description

Technical Field

[0001] The invention belongs to the technical field of animal feed, and in particular relates to a feed for improving sperm quality after freezing, and a preparation method and application thereof. Background Art

[0002] Semen quality is a core indicator of reproductive health in male animals. The quality of semen directly affects fertility. Indicators such as sperm motility and movement parameters, sperm plasma membrane integrity, sperm acrosome integrity rate, and sperm mitochondrial membrane potential are important factors in measuring fertility.

[0003] Sperm cryopreservation is also called frozen semen. The production of frozen semen of breeding male rabbits is one of the core technologies of modern rabbit farming. It can efficiently utilize the genetic resources of high-quality breeding male rabbits, avoid inbreeding, and reduce the inbreeding coefficient of the group. In addition, it can be mated at any time without being affected by the condition and age of the male rabbit, thereby improving breeding efficiency. Secondly, it can also reduce the number of male rabbits raised and reduce breeding costs.

[0004] The semen quality of breeding male rabbits is easily affected by the environmental temperature and humidity, resulting in reduced sperm motility, decreased semen quality, and low breeding rate. Especially from June to October each year, semen motility is mostly concentrated between 0-30%, and sperm motility exceeding 60% is rare. The breeding rate is as low as 30%, which seriously affects the output of meat rabbits and the market consumption is in short supply. At the same time, it affects the quality of frozen semen and the breeding rate after freezing.

[0005] Currently, the main method for improving semen quality and motility is to increase the protein content in the feed. However, blindly increasing protein content can increase kidney burden, cause digestive and metabolic problems, and increase economic costs. Therefore, an appropriate ratio of protein and other trace elements is crucial. Providing a feed with a reasonable composition and ratio of protein and trace minerals is crucial for improving sperm motility, semen quality indicators, and breeding rates in meat rabbits between June and October, both fresh and frozen semen. Summary of the Invention

[0006] In view of this, the object of the present invention is to provide a feed for improving sperm quality after frozen sperm, and a preparation method and application thereof.

[0007] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0008] The invention provides a feed for improving sperm quality of breeding male rabbits and conception rate of female rabbits. The feed comprises the following ingredients in parts by weight: 10 to 20 parts of corn, 50 to 60 parts of soybean meal, 12 to 20 parts of fish meal, 3 to 7 parts of premix and 0.1 to 0.3 part of vitamin E.

[0009] The present invention also provides a method for preparing the feed, comprising the following steps:

[0010] Corn, soybean meal, fish meal, premix and vitamin E are uniformly mixed to obtain a mixture, and the mixture is mixed with water to obtain the feed.

[0011] Preferably, the mass ratio of the mixture to water is 100:2-5.

[0012] The present invention also provides application of the feed or the feed prepared by the preparation method in improving the reproductive performance of rabbits.

[0013] Preferably, the feed is mixed with the early compound feed and fed to breeding male rabbits.

[0014] Preferably, the mass ratio of the feed to the early compound feed is 1:5-15.

[0015] Preferably, the feeding amount is 50 to 100 g / day / time, and the feeding frequency is 1 to 2 times / day.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] The feed of the present invention improves the sperm motility of fresh sperm, which reaches more than 70%; improves the sperm motility after freezing, which reaches more than 50%; and improves the sperm acrosome integrity rate, plasma membrane integrity rate and mitochondrial membrane potential of fresh sperm and frozen sperm. DETAILED DESCRIPTION

[0018] The invention provides a feed for improving sperm quality of breeding male rabbits and conception rate of female rabbits. The feed comprises the following ingredients in parts by weight: 10 to 20 parts of corn, 50 to 60 parts of soybean meal, 12 to 20 parts of fish meal, 5 to 10 parts of premix and 0.1 to 0.3 part of vitamin E.

[0019] In the present invention, the feed for improving the sperm quality of breeding male rabbits and the conception rate of female rabbits comprises the following ingredients in parts by weight: 11 to 19 parts of corn, 51 to 59 parts of soybean meal, 13 to 19 parts of fish meal, 6 to 9 parts of premix, and 0.2 part of vitamin E.

[0020] The present invention also provides a method for preparing the feed, comprising the following steps:

[0021] Corn, soybean meal, fish meal, premix and vitamin E are uniformly mixed to obtain a mixture, and the mixture is mixed with water to obtain the feed.

[0022] In the present invention, the mass ratio of the mixture to water is preferably 100:2-5, more preferably 100:3-4.

[0023] The present invention also provides application of the feed or the feed prepared by the preparation method in improving the reproductive performance of rabbits.

[0024] In the present invention, the feed is mixed with the early compound feed and fed to breeding male rabbits; the mass ratio of the feed to the early compound feed is preferably 1:5-15, more preferably 1:8-12; the early compound feed is preferably a complete granular feed for rabbits; the feeding amount is preferably 50-100 g / day / time, more preferably 60-90 g / day / time, and even more preferably 75 g / day / time; the feeding amount varies due to the adult age of different rabbit species. Taking a 5-month-old Fujian yellow rabbit as an example, the daily feeding amount is 120 g, which can be appropriately increased by 20 g every 15 days, and semen collection training is started. After it reaches adulthood (8 months old), it can be increased to 200 g per day; the number of feedings is preferably 1-2 times / day, and more preferably 2 times / day.

[0025] The technical solutions provided by the present invention are described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0026] Example 1

[0027] A feed for improving the sperm quality of breeding male rabbits and the conception rate of female rabbits is composed of the following ingredients: 16 kg of corn, 57 kg of soybean meal, 17 kg of fish meal, 10 kg of premix (purchased from Zhengzhou Tuduoduo Feed Technology Co., Ltd.) and 0.2 kg of vitamin E.

[0028] The preparation method of the feed is as follows: the powders of the above ingredients are weighed respectively, mixed evenly, 3 kg of pure water is added per 100 kg, the mixture is stirred and mixed evenly, and the feed is prepared by a feed pelletizer.

[0029] Example 2

[0030] A feed for improving the sperm quality of breeding male rabbits and the conception rate of female rabbits is composed of the following ingredients: 19 kg of corn, 55 kg of soybean meal, 18 kg of fish meal, 8 kg of premix (purchased from Zhengzhou Tuduoduo Feed Technology Co., Ltd.) and 0.2 kg of vitamin E.

[0031] The preparation method of the feed is as follows: the powders of the above ingredients are weighed respectively, mixed evenly, 3 kg of pure water is added per 100 kg, the mixture is stirred and mixed evenly, and the feed is prepared by a feed pelletizer.

[0032] Example 3

[0033] A feed for improving the sperm quality of breeding male rabbits and the conception rate of female rabbits is composed of the following ingredients: 10 kg of corn, 50 kg of soybean meal, 12 kg of fish meal, 5 kg of premix (purchased from Zhengzhou Tuduoduo Feed Technology Co., Ltd.) and 0.1 kg of vitamin E.

[0034] The preparation method of the feed is as follows: the powders of the above ingredients are weighed respectively, mixed evenly, 2 kg of pure water is added per 100 kg, the mixture is stirred and mixed evenly, and the feed is prepared by a feed pelletizer.

[0035] Example 4

[0036] A feed for improving the sperm quality of breeding male rabbits and the conception rate of female rabbits, comprising the following ingredients: 20 kg of corn, 60 kg of soybean meal, 20 kg of fish meal, 10 kg of premix (purchased from Zhengzhou Tuduoduo Feed Technology Co., Ltd.) and 0.3 kg of vitamin E;.

[0037] The preparation method of the feed is as follows: the powders of the above ingredients are weighed respectively, mixed evenly, 5 kg of pure water is added per 100 kg, the mixture is stirred and mixed evenly, and the feed is prepared by a feed pelletizer.

[0038] Experimental Example 1

[0039] In August 2023, 100 male black rabbits (8 months old) from Southwest Fujian were raised in the rabbit breeding center of the Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences. The rabbit house environment was a steel-structured insulated rabbit house with a 650m 2. Before the experiment, the breeding house was completely cleaned, the rabbit cages were disinfected, and the ground was cleaned and disinfected. In the early stage, the rabbits were fed with 572 type complete granular feed for growing rabbits. Before grouping, the rabbits were dewormed, immunized, and injected and divided into two groups according to sperm motility, with 50 male rabbits in each group. After grouping, the control group was fed with 572 type complete granular feed for growing rabbits (purchased from Liaocheng Jiashishi Feed Co., Ltd.), and the experimental group was fed with the feed prepared in Example 1 and 572 type complete granular feed for growing rabbits in a mass ratio of 1:10 to obtain a mixed feed. The feeding amount of the control group and the experimental group was 75g / day / time, and the rabbits were fed twice a day with free access to food and water. During the experiment, the health status of the experimental rabbits was observed regularly and detailed records were kept. After feeding for 15 days, semen was collected from both the control group and the experimental group by the pseudo-vaginal method, the semen volume was recorded, and indicators such as sperm motility and sperm motility parameters, sperm plasma membrane integrity, and acrosome integrity rate were observed. After the next semen collection, dead sperm were removed from the semen sample. The remaining semen was mixed and frozen according to the process. Fresh semen was incubated and added with freezing diluent. Sperm with qualified motility was used to prepare frozen semen. The semen was then dispensed into cryovials and gradually cooled to liquid nitrogen for storage. The semen in the cryovials removed from the liquid nitrogen was thawed in a water bath. After thawing, sperm motility and sperm performance parameters, sperm plasma membrane integrity, and acrosome integrity were also measured.

[0040] Experimental results: as shown in Tables 1 and 2.

[0041] Table 1 Effect of feeding rabbit sperm motility and motility with the fresh semen prepared in Example 1

[0042]

[0043]

[0044] As can be seen from Table 1, after feeding the feed prepared in Example 1, the semen volume and sperm motility of the rabbits in the test group were significantly improved, and other motility parameters were also significantly higher than those in the control group.

[0045] Table 2 Effect of the feed prepared in Example 1 on the quality indexes of rabbit sperm (%)

[0046]

[0047] As can be seen from Table 2, after feeding the feed prepared in Example 1, the acrosome integrity rate, plasma membrane integrity and mitochondrial membrane potential of the fresh sperm and frozen sperm of the rabbits in the test group were significantly higher than those in the control group. The acrosome integrity rate of the sperm of the fresh sperm and frozen sperm increased by 41.2% and 98.7% respectively, the plasma membrane integrity rate increased by 45.1% and 78.5% respectively, and the mitochondrial membrane potential increased by 60.5% and 66.6% respectively compared with the control group.

[0048] Experimental Example 2

[0049] In July 2024, 100 adult male Fujian yellow rabbits (6 months old) were raised in the Yuhuashan Natural Ecological Agriculture Experimental Field, with 50 rabbits in the control group and the experimental group. The control group was fed with complete pellet feed for rabbits (purchased from Jinan Shuangyi Feed Co., Ltd.), and the experimental group rabbits were fed with a mixed feed obtained by mixing the feed prepared in Example 1 and the complete pellet feed for rabbits in a mass ratio of 1:10. The feeding amount of the control group and the experimental group was 75g / day / time. Before the test, the breeding house was completely cleaned, the rabbit cages were disinfected, and the ground was cleaned and disinfected. The pre-test period was 7 days, during which grouping, deworming, and immunization were carried out. Semen collection began 15 days after the main test feeding. Feed twice a day, free access to food and water. During the test, the health status of the test rabbits was regularly observed and detailed records were kept. After 15 days of feeding, semen was collected from both the control and experimental groups using the pseudo-vaginal method. Semen volume was recorded, and indicators such as sperm motility and motility parameters, sperm plasma membrane integrity, and acrosome integrity were measured. After the next semen collection, the semen samples, after removing dead sperm, were mixed and frozen according to the rabbit semen freezing process. Fresh semen was incubated and added with freezing diluent. Sperm with qualified motility was used to prepare frozen semen. The semen was then dispensed into cryovials and gradually cooled to liquid nitrogen for storage. The semen in the cryovials removed from the liquid nitrogen was thawed in a water bath. After thawing, indicators such as sperm motility and motility parameters, sperm plasma membrane integrity, and acrosome integrity were also measured.

[0050] Experimental results: as shown in Tables 3 and 4.

[0051] Table 3 Effects of feeding the feed prepared in Example 2 on sperm motility and motility parameters

[0052]

[0053]

[0054] As can be seen from Table 3, after feeding the feed prepared in Example 2, the sperm motility and movement parameters of the fresh and frozen semen of the rabbits in the test group were significantly improved compared with those in the control group.

[0055] Table 4 Effect of feeding the feed prepared in Example 2 on the quality indexes of rabbit sperm (%)

[0056]

[0057] As can be seen from Table 4, after feeding the feed prepared in Example 2, the sperm acrosome integrity rate, plasma membrane integrity and mitochondrial membrane potential of the fresh semen and frozen semen of the experimental group rabbits were significantly higher than those of the control group. The sperm acrosome integrity rate of fresh semen and frozen semen increased by 44.6% and 163.0% respectively, the plasma membrane integrity rate increased by 60.5% and 33.9% respectively, and the mitochondrial membrane potential increased by 61.8% and 62.8% respectively compared with the control group.

[0058] It can be seen from the above embodiments and experimental examples that the feed of the present invention improves sperm motility of fresh sperm, sperm motility after freezing, sperm acrosome integrity rate, plasma membrane integrity rate, and mitochondrial membrane potential of fresh sperm and frozen sperm.

[0059] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. A feed for improving the sperm quality of breeding male rabbits and the conception rate of female rabbits, characterized in that: The invention comprises the following ingredients in parts by weight: 10 to 20 parts of corn, 50 to 60 parts of soybean meal, 12 to 20 parts of fish meal, 3 to 7 parts of premix and 0.1 to 0.3 part of vitamin E.

2. The method for preparing the feed according to claim 1, wherein The following steps are involved: Corn, soybean meal, fish meal, premix and vitamin E are uniformly mixed to obtain a mixture, and the mixture is mixed with water to obtain the feed.

3. The preparation method according to claim 2, characterized in that The mass ratio of the mixture to water is 100:2-5.

4. Use of the feed according to claim 1 or the feed prepared by the preparation method according to claims 2 to 3 in improving the reproductive performance of rabbits.

5. The use according to claim 4, characterized in that The feed is mixed with the early compound feed and fed to breeding male rabbits.

6. The use according to claim 5, characterized in that The mass ratio of the feed to the early compound feed is 1:5-15.

7. The use according to claim 5, characterized in that The feeding amount is 50 to 100 g / day / time, and the feeding frequency is 1 to 2 times / day.

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