Tool and method for constructing mouse intrauterine adhesion model

Abstract

The invention provides a construction tool and method of a mouse intrauterine adhesion model, and relates to the technical field of medical tests. The tool comprises a scraper, a piezoelectric probe, a connecting handle, a limiting device and a fixed sleeve, the scraping shovel is a round blunt end and is connected to the connecting handle; the connecting handle can extend into the fixed sleeve; the limiting device is arranged on the connecting handle and can adjust the length of the portion, extending into the fixing sleeve, of the connecting handle. A piezoelectric sensor is arranged in the piezoelectric probe and can be connected to the connecting handle to feed back contact pressure. The method comprises the steps that a fixing sleeve stretches into an incision, and a target uterine cavity section is exposed; the connecting handle extends into the fixed sleeve, and the extending depth of the connecting handle is adjusted through the piezoelectric probe; and after the stretching depth is set, the spatula is mounted at the top of the connecting handle, so that the spatula scrapes the inner wall of the uterine cavity. According to the method, the damage depth and range can be accurately controlled, and the consistency, standardization and repeatability of the model are ensured.

Description

Technical Field

[0001] The present invention relates to the field of medical experiment technology, and in particular to a tool and method for constructing a mouse intrauterine adhesion model. Background Art

[0002] Intrauterine adhesions (IUA) are damage to the endometrium that leads to complete or partial obstruction of the uterine cavity or cervical canal. Generally speaking, a common cause of IUA is artificial damage to the uterine cavity during surgery, such as curettage, cesarean section, and myomectomy, which may damage the basal layer of the endometrium.

[0003] There are two main methods for establishing intrauterine adhesion models in mice: mechanical injury combined with lipopolysaccharide perfusion and chemical injury. The latter method uses a minimally invasive approach to create an intrauterine adhesion model that matches clinical injury characteristics. However, its disadvantages include the complexity of the surgical procedure, which requires precise manipulation and high technical skills. Furthermore, individual variability in experimental procedures can lead to inconsistent modeling results.

[0004] The disadvantages of chemical injury methods (such as ethanol perfusion) are that they may affect endometrial regeneration, which may limit subsequent fertility studies. In addition, it is difficult to control the dose and time of chemical injury, which may affect the consistency and reproducibility of the model.

[0005] In summary, the mechanical injury combined with lipopolysaccharide perfusion method has advantages in simulating clinical injury characteristics and the replicability of the model, but the operation is complicated and has high technical requirements. The chemical injury method is simple to operate and low in cost, but it may affect the regeneration of the endometrium and is more difficult to control the degree of injury. In response to the above problems, the present surgical invention improves the tools for constructing a mechanical injury model, avoiding problems such as the adverse effects of surgical instruments such as syringe needles penetrating the uterus on endometrial regeneration. At the same time, the surgical operation is improved to make the degree of endometrial damage more uniform, and it is easy to obtain an animal disease model that is closer to human diseases. The present surgical invention improves the convenience and operability of the operation and avoids model differences caused by different experimental operators. Summary of the Invention

[0006] The purpose of the present invention is to overcome the shortcomings of the prior art and provide a tool for constructing a mouse intrauterine adhesion model, which is specifically achieved through the following technical solutions:

[0007] The present invention provides a tool for constructing a mouse intrauterine adhesion model, comprising a spatula, a piezoelectric probe, a connecting handle, a limiting device and a fixing sleeve;

[0008] The bottom of the scraper can be detachably connected to the top of the connecting handle, and the top of the scraper is a rounded blunt head; the connecting handle can be inserted into the fixed sleeve; the limiting device is provided on the connecting handle and can adjust the length of the connecting handle inserted into the fixed sleeve;

[0009] The bottom of the piezoelectric probe can be detachably connected to the top of the connecting handle. The piezoelectric probe is equipped with a piezoelectric sensor to feedback contact pressure.

[0010] Optionally or preferably, the limiting device is an elliptical ring structure, and a thread is provided on the inner ring of the limiting device; a matching thread is provided on the outer wall of the connecting handle; the limiting device is sleeved on the connecting handle, and the installation position of the limiting device on the connecting handle can be adjusted by rotation.

[0011] Optionally or preferably, the fixed sleeve is a transparent flexible pipe; and the fixed sleeve is a tapered tube, with a smaller diameter at the end close to the mouse and a larger diameter at the end away from the mouse, which can facilitate the insertion of the connecting handle and improve the accuracy of the operation.

[0012] Optionally or preferably, the end of the fixing sleeve away from the mouse can be mounted and fixed on a bracket.

[0013] Optionally or preferably, a conical slot and a locking joint are provided on the top of the connecting handle; and a protrusion that matches the conical slot is provided on the bottom of the scraper.

[0014] Optionally or preferably, a conical slot and a locking joint are provided on the top of the connecting handle; and a protrusion that matches the conical slot is provided on the bottom of the piezoelectric probe.

[0015] The present invention provides a method for constructing a mouse intrauterine adhesion model, comprising the following steps:

[0016] S1. Select mice in estrus;

[0017] S2. Anesthetize the mice and prepare their skin;

[0018] S3. Make an opening in the mouse's flank near the cervix to expose one side of the uterus.

[0019] S4. Make an incision on the mouse uterus;

[0020] S5. Insert the fixed cannula through the incision to expose the target uterine cavity segment; insert the connecting handle into the fixed cannula, and adjust the insertion depth of the connecting handle using the piezoelectric probe; after setting the insertion depth, install the spatula on the top of the connecting handle and scrape the spatula on the inner wall of the uterine cavity;

[0021] S6. Remove the spatula, piezoelectric probe, connecting handle, and fixing cannula from the mouse body;

[0022] S7. Suture the incision.

[0023] Optionally or preferably, when performing the scraping in step S5, the scraper is used to scrape the inner wall of the uterine cavity once in each of the four directions of up, down, left, and right.

[0024] Based on the above technical solution, the following technical effects can be produced:

[0025] 1. Accurately control the damage depth and range to ensure model consistency and standardization.

[0026] 2. Reduce non-target tissue damage and improve operational safety.

[0027] 3. Improve operational convenience and repeatability. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying any creative work.

[0029] Figure 1 It is a working schematic diagram of the present invention (top view);

[0030] Figure 2 It is a structural schematic diagram (front view) of the present invention;

[0031] Figure 3 Schematic diagram of the structure of the piezoelectric probe in the present invention;

[0032] Figure 4 Schematic diagram of the structure of the scraper in the present invention;

[0033] Figure 5 Schematic diagram of the structure of the limiting device in the present invention;

[0034] In the figure: 1-scraper, 2-piezoelectric probe, 3-connecting handle, 4-limiting device, 5-fixing sleeve, 6-bracket. DETAILED DESCRIPTION

[0035] It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0036] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.

[0037] Example 1:

[0038] like Figure 1-Figure 5 As shown:

[0039] The present invention provides a tool for constructing a mouse intrauterine adhesion model, comprising a spatula 1, a piezoelectric probe 2, a connecting handle 3, a limiting device 4 and a fixing sleeve 5;

[0040] The bottom of the scraper 1 can be detachably connected to the top of the connecting handle 3, and the top of the scraper 1 is a rounded blunt head; the connecting handle 3 can be inserted into the fixed sleeve 5; the limiting device 4 is provided on the connecting handle 3 and can adjust the length of the connecting handle 3 inserted into the fixed sleeve 5;

[0041] The bottom of the piezoelectric probe 2 can be detachably connected to the top of the connecting handle 3. The piezoelectric probe 2 has a built-in piezoelectric sensor that can feedback contact pressure.

[0042] The limiting device 4 is an elliptical ring structure, and a thread is provided on the inner ring of the limiting device 4; a thread matching the thread is provided on the outer wall of the connecting handle 3; the limiting device 4 is sleeved on the connecting handle 3, and the limiting device 4 can adjust the installation position on the connecting handle 3 by rotation.

[0043] Furthermore, the fixed sleeve 5 is a transparent flexible pipe, which is convenient for the operator to observe; and the fixed sleeve 5 is a tapered tube, with a smaller diameter at the end close to the mouse and a larger diameter at the end away from the mouse, which can facilitate the insertion of the connecting handle 3 and improve the accuracy of the operation.

[0044] Furthermore, the end of the fixing sleeve 5 away from the mouse can be mounted and fixed on the bracket 6.

[0045] Furthermore, the top of the connecting handle is provided with a conical groove and a locking joint; the bottom of the scraper is provided with a protrusion that matches the conical groove.

[0046] Furthermore, the top of the connecting handle is provided with a tapered slot and a locking joint; the bottom of the piezoelectric probe is provided with a protrusion that matches the tapered slot.

[0047] The present invention provides a method for constructing a mouse intrauterine adhesion model, comprising the following steps:

[0048] S1. Select mice in estrus;

[0049] S2. Anesthetize the mice and prepare their skin;

[0050] S3. Make an opening in the mouse's flank near the cervix to expose one side of the uterus.

[0051] S4. Make an incision on the mouse uterus;

[0052] S5. Insert the fixed cannula through the incision to expose the target uterine cavity segment; insert the connecting handle into the fixed cannula, and adjust the insertion depth of the connecting handle using the piezoelectric probe; after setting the insertion depth, install the spatula on the top of the connecting handle and scrape the spatula on the inner wall of the uterine cavity;

[0053] S6. Remove the spatula, piezoelectric probe, connecting handle, and fixing cannula from the mouse body;

[0054] S7. Suture the incision.

[0055] In this embodiment, when performing the scraping in step S5, the scraper is used to scrape the inner wall of the uterine cavity once in each of the four directions of up, down, left, and right.

[0056] The working principle and advantages of this embodiment are as follows:

[0057] 1. The limiting device 4 in this embodiment can adjust the depth of the connecting handle 3 inserted into the fixed sleeve, thereby controlling the insertion length of the scraper 1, ensuring that each scraping reaches the predetermined position, avoiding excessively deep scraping that damages the muscle layer or excessively shallow scraping that results in ineffective results.

[0058] 2. The elliptical limiting device 4 of this embodiment enables the operator to precisely scrape in the four directions of up, down, left, and right. This facilitates the creation of uniform, controllable localized endometrial damage within a designated uterine cavity segment, significantly improving the homogeneity of adhesion formation between different individuals and enhancing the reliability of experimental data.

[0059] 3. Before placing the spatula 1, perform a preliminary probe with the piezoelectric probe 2. The piezoelectric sensor provides real-time feedback on contact pressure, allowing the operator to adjust the force accordingly to prevent the spatula from accidentally penetrating the uterine wall due to excessive force, causing unintended heavy bleeding or damage to abdominal organs.

[0060] 4. The top of the spatula 1 in this embodiment is a rounded blunt tip, which can only damage the surface endothelium, causing inflammation and adhesion, while avoiding sharp perforation;

[0061] 5. The fixed sleeve 5 of this embodiment is tapered, which helps the spatula 1 to accurately align and enter the relatively narrow target uterine cavity area, reducing deviation.

[0062] The foregoing description is merely a preferred embodiment of the present invention. It should be understood that the present invention is not limited to the form disclosed herein and should not be construed as excluding other embodiments. Rather, the present invention can be used in various other combinations, modifications, and environments and can be modified within the scope of the concept described herein through the above teachings or techniques or knowledge in the relevant field. Modifications and variations made by those skilled in the art that do not depart from the spirit and scope of the present invention are intended to be protected by the appended claims.

Claims

1. A tool for constructing a mouse intrauterine adhesion model, characterized by: It includes a scraper, a piezoelectric probe, a connecting handle, a limiting device and a fixing sleeve; The bottom of the scraper can be detachably connected to the top of the connecting handle, and the top of the scraper is a rounded blunt head; the connecting handle can be inserted into the fixed sleeve; the limiting device is provided on the connecting handle and can adjust the length of the connecting handle inserted into the fixed sleeve; The bottom of the piezoelectric probe can be detachably connected to the top of the connecting handle. The piezoelectric probe is equipped with a piezoelectric sensor to feedback contact pressure.

2. The tool for constructing a mouse intrauterine adhesion model according to claim 1, characterized in that: The limiting device is an elliptical ring structure, and a thread is provided on the inner ring of the limiting device; a matching thread is provided on the outer wall of the connecting handle; the limiting device is sleeved on the connecting handle, and the installation position of the limiting device on the connecting handle can be adjusted by rotation.

3. The tool for constructing a mouse intrauterine adhesion model according to claim 1, wherein: The fixed sleeve is a transparent flexible pipe; and the fixed sleeve is a tapered tube, with a smaller diameter at the end close to the mouse and a larger diameter at the end away from the mouse, which facilitates the insertion of the connecting handle and improves the accuracy of the operation.

4. The tool for constructing a mouse intrauterine adhesion model according to claim 1, wherein: The end of the fixing sleeve away from the mouse can be mounted and fixed on the bracket.

5. The tool for constructing a mouse intrauterine adhesion model according to claim 1, wherein: The top of the connecting handle is provided with a conical slot and a locking joint; the bottom of the scraper is provided with a protrusion matched with the conical slot.

6. The tool for constructing a mouse intrauterine adhesion model according to claim 1, wherein: The top of the connecting handle is provided with a conical groove and a locking joint; the bottom of the piezoelectric probe is provided with a protrusion matched with the conical groove.

7. A method for constructing a mouse intrauterine adhesion model, characterized by: Using the construction tool as claimed in claim 1, comprising the following steps: S1. Select mice in estrus; S2. Anesthetize the mice and prepare their skin; S3. Make an opening in the mouse's flank near the cervix to expose one side of the uterus. S4. Make an incision on the mouse uterus; S5. Insert the fixed cannula through the incision to expose the target uterine cavity segment; insert the connecting handle into the fixed cannula, and adjust the insertion depth of the connecting handle using the piezoelectric probe; after setting the insertion depth, install the spatula on the top of the connecting handle and scrape the spatula on the inner wall of the uterine cavity; S6. Remove the spatula, piezoelectric probe, connecting handle, and fixing cannula from the mouse body; S7. Suture the incision.

8. The method for constructing a mouse intrauterine adhesion model according to claim 1, wherein: When performing the scraping in step S5, the scraper is used to scrape the inner wall of the uterine cavity once in each of the four directions of up, down, left, and right.

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