A skin care composition with pore-shrinking, skin-smoothing and anti-wrinkle effects, and a preparation method and application thereof

Abstract

The application discloses a skin care composition with pore-shrinking, skin tenderizing and wrinkle-resisting effects and a preparation method and application thereof, and particularly belongs to the technical field of daily-use cosmetics. The composition contains the following components in mass fractions: 5-8 parts of Astragalus membranaceus (Fisch.) Bunge root extract, 0.15-0.2 parts of raspberry ketone glucoside, 3-5 parts of sea algae extract, 0.8-1 part of fire jujube fruit extract and 20-30 parts of Saccharomyces bitymus fermentation product filtrate, and each component is prepared through specific process extraction; the composition provided by the application has pore-shrinking, wrinkle-resisting, skin tightening, oil-controlling and other effects, and can be used for preparing various skin care products such as essence and emulsion.

Description

Technical Field

[0001] This invention belongs to the technical field of daily cosmetics, specifically relating to a skin care composition with the effects of shrinking pores, rejuvenating skin, and reducing wrinkles, as well as its preparation method and application. Background Technology

[0002] Studies have found that the causes of enlarged pores include: 1. Excessive sebum secretion leads to the proliferation of lipophilic microorganisms, which in turn causes hair follicle blockage and enlarged pores; 2. Loss of skin collagen results in insufficient skin firmness, which in turn leads to enlarged pores.

[0003] While existing products offer diverse benefits, single-ingredient products often have limited effectiveness and struggle to meet specific needs. For instance, some anti-wrinkle products primarily target collagen loss but are ineffective at controlling oil production and thus fail to address issues like enlarged pores. Furthermore, the lack of synergistic effects among some products significantly diminishes their overall effectiveness.

[0004] Therefore, there is an urgent need to develop a skincare composition that integrates multiple functions and synergistic effects of ingredients to meet the market demand for high-quality, comprehensive skincare products. Summary of the Invention

[0005] In view of the shortcomings of the prior art, the purpose of this invention is to provide a skin care composition, its preparation method and application. This composition can reduce skin lipid synthesis, thereby reducing pore blockage and tightening pores, while promoting collagen synthesis, firming the skin, and making the skin tender and wrinkle-free.

[0006] To achieve the above objectives, the present invention discloses the following technical solutions:

[0007] In a first aspect, the present invention provides a skin care composition, wherein, by weight parts, the composition contains the following components:

[0008]

[0009] Preferably, the preparation method of the Astragalus membranaceus root extract includes the following steps:

[0010] Step 1-1. Place the dried Astragalus membranaceus root in a grinder and grind it through a 30-mesh sieve to obtain Astragalus membranaceus root powder;

[0011] Step 1-2. Mix Astragalus membranaceus root powder with deionized water at a mass-to-volume ratio of 1:20-30 g / mL, and sonicate at 25-30 kHz frequency and 50-60 ℃ for 20-30 min. After sonication, continue soaking at 50-60 ℃ for 12-16 h, and pass through a 300-400 mesh sieve to obtain crude extract.

[0012] Steps 1-3. Place the crude extract in a vacuum concentrator and concentrate it to 1 / 3 of its original volume at 30-50 kPa and 60-70℃ to obtain Astragalus membranaceus root extract.

[0013] Preferably, the preparation method of the seaweed extract includes the following steps:

[0014] Step 2-1. Take a certain amount of seaweed and mix it with deionized water at a volume-to-mass ratio of 1:20-30 g / mL. Then, break the cell wall and pulverize it at 20000-30000 r / min for 10-15 min to obtain seaweed homogenate.

[0015] Step 2-2. Heat the seaweed homogenate to 70-80℃ and keep it at that temperature for 50-60 minutes. After cooling the temperature to 25-35℃, centrifuge to obtain the supernatant. Add a 95 v / v% ethanol aqueous solution to the supernatant, mix and soak for 24-26 hours, and centrifuge to obtain the precipitate.

[0016] Step 2-3. Place the precipitate obtained from the separation in step 2-2 in an oven at 50-60℃ and dry it until there is no alcohol odor to obtain seaweed extract.

[0017] Preferably, the preparation method of the firethorn fruit extract includes the following steps:

[0018] Step 3-1. Take a certain amount of dried firethorn fruit and put it into a pulverizer to pulverize it through a 60-mesh sieve to obtain firethorn fruit powder. Moisten the firethorn fruit powder with deionized water so that the water content of the firethorn fruit powder reaches 40-50% to obtain a solid fermentation substrate. Sterilize it for later use.

[0019] Step 3-2. Take samples with a live bacteria count ≥ 1 × 10⁻⁶ 8 The *Saccharomyces cerevisiae* culture solution (CFU / mL) was sprayed onto a solid fermentation substrate at 15-20% of the substrate mass. The substrate was then cultured at 28-30℃, an aeration rate of 0.5-0.7 L / min·kg, and a stirring speed of 3-7 r / min for 72-80 h to obtain the fermentation material.

[0020] Step 3-3. Mix the fermentation material with a 70-80 v / v% ethanol aqueous solution at a ratio of 1:15-20 g / mL, reflux and extract three times at 70-80℃ for 1-1.2 h each time, combine the extracts, concentrate the filtrate under reduced pressure to 1 / 3 of the original volume, and finally vacuum dry to a water content ≤10% to obtain the firethorn fruit extract.

[0021] Secondly, the present invention provides the application of the skin care composition described in the first aspect in the preparation of skin care products with anti-wrinkle, firming and oil-controlling effects.

[0022] Thirdly, the present invention provides an essence with the effects of shrinking pores, rejuvenating skin, and reducing wrinkles, wherein the essence contains the skin care composition described in the first aspect;

[0023] Preferably, the amount of the skincare composition added to the serum is 5-10 wt%.

[0024] More preferably, the essence also contains preservatives, antioxidants, moisturizers, and solvents.

[0025] Fourthly, the present invention provides a method for preparing the serum described in the third aspect, the method comprising the following steps:

[0026] The skincare composition, preservative, antioxidant, moisturizer, and solvent are mixed and stirred evenly to obtain the essence.

[0027] In this invention:

[0028] The water-extracted Astragalus membranaceus root extract contains active ingredients such as polysaccharides, flavonoids, saponins, and amino acids, which have whitening, spot-fading, anti-aging, antioxidant, and anti-inflammatory effects.

[0029] Raspberry ketone glucoside has strong antioxidant properties, effectively scavenging free radicals and reducing oxidative damage to skin cells. Simultaneously, it exhibits strong inhibitory activity against tyrosinase, effectively suppressing melanin production. While there is limited research on the anti-inflammatory and oil-controlling effects of raspberry ketone glucoside, studies have found that combining raspberry ketone with anti-inflammatory agents enhances the anti-inflammatory effects and bioavailability of the agents. Therefore, this invention creatively incorporates raspberry ketone glucoside into the formulation, revealing its promoting effect on oil control.

[0030] Seaweed extract obtained through water extraction and alcohol precipitation is rich in seaweed polysaccharides. These polysaccharide active substances have excellent antioxidant, anti-inflammatory, and moisturizing effects, promote the synthesis of skin collagen, and promote the repair of the skin barrier. At the same time, seaweed extract also contains cytosine-like amino acids, which have strong antioxidant and anti-ultraviolet damage effects.

[0031] The firethorn fruit extract obtained through solid-state fermentation with *Saccharomyces cerevisiae* is rich in flavonoids. These flavonoids can shrink pores, improve uneven skin tone, provide antioxidant benefits, scavenge free radicals, delay skin aging, and brighten skin tone. They also promote collagen synthesis, increasing skin elasticity and firmness. Furthermore, they possess anti-inflammatory properties, relieving skin redness, itching, and other discomforts, making them suitable for sensitive skin. Compared to unfermented or other yeast-fermented firethorn fruit extracts, the firethorn fruit extract provided by this invention has a higher total flavonoid content, more active substances, and better effects.

[0032] This invention combines Astragalus membranaceus root extract, raspberry ketone glucoside, seaweed extract, firethorn fruit extract, and Bifida ferment filtrate to form a complete and comprehensive efficacy ingredient package. It can be used alone as an efficacy ingredient after dilution with a solvent, or it can be compounded with excipients to form any dosage form such as serum, lotion, spray, ointment, or cream, etc., and has good compatibility.

[0033] The beneficial effects of this invention are:

[0034] The skin care composition and skin care products provided by this invention have significant anti-wrinkle, firming and oil-controlling effects, can effectively shrink pores and improve skin elasticity, and the various ingredients work synergistically to enhance the overall effect. Detailed Implementation

[0035] The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0036] To further illustrate the present invention, the following embodiments are provided for detailed description. The raw materials used in the following embodiments and comparative examples of the present invention are all commercially available products.

[0037] In this invention:

[0038] Astragalus membranaceus root extract: Astragalus membranaceus root extract;

[0039] Gloiopeltis Furacata extract: Gloiopeltis Furacata extract;

[0040] Pyracantha fruit extract: Pyracantha fortuneana fruit extract;

[0041] Bifida ferment filtrate: purchased from Gansu Yiweisen Pharmaceutical Technology Co., Ltd.;

[0042] Saccharomyces paradoxus, Latin name: Saccharomyces paradoxus, accession number CGMCC 2.5692, purchased from China General Microbiological Culture Collection Center;

[0043] Saccharomyces cerevisiae, Latin name, accession number CGMCC 2.1527, purchased from China General Microbiological Culture Collection Center.

[0044] Preparation of Astragalus membranaceus root extract:

[0045] Step 1-1. Dry the roots of Astragalus membranaceus at 50°C to constant weight using a constant temperature drying oven, and then pulverize them in a pulverizer and pass them through a 30-mesh sieve to obtain Astragalus membranaceus root powder.

[0046] Step 1-2. Mix Astragalus membranaceus root powder with deionized water at a mass-volume ratio of 1:20 g / mL and soak. Ultrasonically treat at 28 kHz frequency and 55 ℃ for 20-30 min. After ultrasonic treatment, continue soaking at 55 ℃ for 14 h. Pass through a 300-mesh sieve to obtain crude extract.

[0047] Steps 1-3. Place the crude extract in a vacuum concentrator and concentrate it to 1 / 3 of its original volume at 40 kPa and 60°C to obtain Astragalus membranaceus root extract.

[0048] Preparation of seaweed extract:

[0049] Step 2-1. Take a certain amount of seaweed and deionized water and mix them at a volume-to-mass ratio of 1:30 g / mL. Then, break the cell wall and pulverize the mixture at 25000 r / min for 10 min to obtain a seaweed homogenate.

[0050] Step 2-2. Heat the seaweed homogenate to 80℃ and keep it at that temperature for 60 minutes. After the temperature drops to 35℃, centrifuge to obtain the supernatant. Add a 95% v / v ethanol aqueous solution to the supernatant and mix and soak for 26 hours. Crystallized precipitate is produced during the soaking process. After the soaking is completed, centrifuge to obtain the precipitate.

[0051] Step 2-3. Place the precipitate obtained from the separation in step 2-2 in a 60℃ oven and dry until there is no alcohol odor to obtain seaweed extract.

[0052] Preparation of Pyracantha fruit extract:

[0053] Step 3-1. Dry the firethorn fruit at 50℃ to constant weight using a constant temperature drying oven. Place the dried firethorn fruit in a pulverizer and pulverize it through a 60-mesh sieve to obtain firethorn fruit powder. Moisten the firethorn fruit powder with deionized water to make the moisture content of the firethorn fruit powder reach 50% to obtain a solid fermentation substrate. Sterilize the fermentation substrate in a 121℃ high-pressure steam cooker for 10 minutes and set aside.

[0054] Step 3-2. Take a live bacteria count of 1×10⁻⁶. 8 The *Saccharomyces cerevisiae* culture at CFU / mL was sprayed onto a solid fermentation substrate at 17% of the substrate mass. The substrate was then cultured at 28°C, an aeration rate of 0.6 L / min·kg, and a stirring speed of 5 r / min for 72 h to obtain the fermentation material.

[0055] Step 3-3. Mix the fermentation material with an 80 v / v% ethanol aqueous solution at a ratio of 1:15 g / mL, reflux and extract three times at 75°C for 1 hour each time, combine the extracts and obtain the filtrate.

[0056] Steps 3-4. Concentrate the filtrate under reduced pressure to 1 / 3 of its original volume, and finally vacuum dry it to a water content of 8% to obtain the firethorn fruit extract.

[0057] Total flavonoid yield:

[0058] The extract filtrate obtained in step 3-3 of the above preparation method is denoted as filtrate ①.

[0059] The extract obtained without fermentation is referred to as filtrate ②. The specific preparation method is as follows: First, the firethorn fruit is dried at 50℃ to constant weight in a constant temperature drying oven. The dried firethorn fruit is then crushed in a pulverizer and passed through a 60-mesh sieve to obtain firethorn fruit powder. The firethorn fruit powder is mixed with an 80 v / v% ethanol aqueous solution at a ratio of 1:15 g / mL. The mixture is then refluxed at 75℃ for 3 times, 1 hour each time. The extracts are combined to obtain filtrate ②.

[0060] The extract obtained after fermentation with Saccharomyces cerevisiae is designated as filtrate ③. The preparation method is the same as that of filtrate ①, except that in step 3-2, *Saccharomyces cerevisiae* is replaced with *Saccharomyces cerevisiae*, and the viable cell count is 1×10⁻⁶. 8 CFU / mL, the rest is the same as filtrate ①.

[0061] The following methods were used to determine the total flavonoids. The differences between filtrate ①, filtrate ②, and filtrate ③ were compared to verify the effect of different preparation processes on the extraction yield of total flavonoids from Pyracantha fortuneana fruit. The specific detection methods are as follows:

[0062] 1. Standard Curve

[0063] Weigh 0.0125 g of rutin standard, dissolve it in ethanol, and dilute to 100 mL to obtain the rutin reference solution. Accurately pipette 0, 2.0, 4.0, 6.0, 8.0, and 10.0 mL of the reference solution into separate 25 mL volumetric flasks. Add 2 mL of 2% aluminum chloride solution and 3 mL of 3% sodium chloride solution, shake well, and develop the color at 25 °C for 5 min. Then, dilute to the mark with 80% ethanol and shake well. Use the 80% ethanol solution as a blank control. Measure the absorbance at a wavelength of 417 nm and plot a standard curve.

[0064] 2. Sample solution to be tested

[0065] Take 1 mL of each of the filtrates ①-③ and place them in a 100 mL volumetric flask. Dilute to the mark with 80 v / v ethanol aqueous solution to obtain the sample solutions 1-3 to be tested.

[0066] 3 Total flavonoid content

[0067] Accurately measure 2 mL of the sample solution to be tested into a 25 mL volumetric flask, add 2 mL of 2% aluminum chloride solution and 3 mL of 3% sodium chloride solution, shake well, and develop color at 25℃ for 5 min. Then, dilute to the mark with 80% ethanol and shake well. Use 80% ethanol solution as a blank control. Measure the absorbance at a wavelength of 417 nm. Calculate the concentration of total flavonoids in the sample solution using the regression equation of the standard curve. Calculate the extraction rate of total flavonoids using the following formula.

[0068]

[0069] In the formula:

[0070] C—Total flavonoid concentration in the sample solution, g / L;

[0071] V—Total volume of filtrate, in L;

[0072] M – Total weight of firethorn fruit powder, in g.

[0073] 4 Test Results

[0074] Table 1 Total Flavonoid Extraction Rate

[0075] Group Total flavonoid extraction rate / % Filtrate ① 4.67 Filtrate ② 2.11 Filtrate ③ 3.42

[0076] 5 Results Analysis

[0077] The results show that the total flavonoid yield of the Pyracantha fruit extract after fermentation with *Saccharomyces cerevisiae* is significantly increased, which is significantly better than that of the Pyracantha fruit extract extracted by solvent extraction alone. It also has advantages over the extract prepared by fermentation with *Saccharomyces cerevisiae*. Therefore, *Saccharomyces cerevisiae* can significantly enrich the active ingredients in Pyracantha fruit, increase the content of active ingredients, and improve the extraction yield of Pyracantha fruit extract.

[0078] Preparation of skincare composition:

[0079] The raw materials were precisely mixed according to the mass ratio in Table 1 to obtain a skin care composition.

[0080] Table 1. Mass ratio of raw materials in skin care composition

[0081] Raw material name Composition 1 Composition 2 Composition 3 Astragalus membranaceus root extract 5 6 8 Raspberry ketone glucoside 0.15 0.18 0.2 seaweed extract 3 4 5 Pyracantha fruit extract 0.8 0.9 1 Bifida ferment filtrate 20 25 30

[0082] Set comparison

[0083] To verify the interaction between the components in the skincare composition and their contribution to the overall formula, a default setting was applied to the formulation of composition 2, and performance tests were conducted for comparison. The specific control settings are shown in Table 2.

[0084] Table 2 Comparative Composition Raw Material Mass Ratio

[0085]

[0086] Performance testing

[0087] 1. Inflammatory factor inhibition test

[0088] 1.1 Experimental Principle

[0089] The production of TNF-α and IL-6 can lead to excessive sebum secretion in the skin. TNF-α can upregulate the expression of sterol regulatory element binding protein 1c (SREBP-1c) mRNA and promote the synthesis of triglycerides in cells. IL-6 can induce the formation of adhesion molecule-1 in the keratin infundibulum of the pilosebaceous gland, thereby enlarging the sebaceous gland and synthesizing lipids. Therefore, detecting the inhibitory performance of the test substance on inflammatory factors can reflect the oil control ability of the test substance.

[0090] 1.2 Test substance

[0091] Test substances: skin care compositions 1-3 and skin care comparison compositions 1-5.

[0092] 1.2 Cytotoxicity Detection

[0093] MTT assay for cytotoxicity: After digestion in the logarithmic growth phase, cells were seeded into 96-well plates. The plates were incubated at 37°C with 5v / v% CO2 for 18–24 h. The supernatant was discarded, and different concentrations of the test substance were added to achieve mass percentages of 0.1%, 0.3%, 0.6%, 1%, 2%, 5%, and 10% in the total system. The plates were then incubated at 37°C with 5v / v% CO2 for further culture. After 18–24 h of cell culture, the supernatant was discarded, and MTT solution (0.5 mg / mL) was added. The mixture was gently mixed and incubated at 37°C in the dark for 4 h. After incubation, the supernatant was discarded, and 150 μL of DMSO was added to each well. The mixture was shaken for 20 min, and the absorbance was measured at 490 nm using a microplate reader. Cell viability was calculated using the following formula:

[0094]

[0095] The results showed that when the mass percentage of the test substance in the cell culture system was ≤2%, the relative cell viability was ≥90%. Therefore, 1% was selected as the safe culture concentration, and the following experiments were conducted.

[0096] 1.3 Detection of inflammatory factor levels

[0097] Untreated cells were used as a blank control, cells treated with only lipopolysaccharide (LPS) were used as a negative control, and the positive control was LPS + dexamethasone. The sample group consisted of LPS + the test substance, as detailed in the table below:

[0098] Table 3

[0099] Grouping Dosage Blank control Cell culture medium without any added substances negative control Cell culture medium containing 0.0005% LPS Positive control Cell culture medium containing 0.0005% LPS and 0.001% dexamethasone Sample group 1 Cell culture medium containing 0.0005% LPS and 1% Composition 1 Sample group 2 Cell culture medium containing 0.0005% LPS and 1% Composition 2 Sample group 3 Cell culture medium containing 0.0005% LPS and 1% composition 3 Sample group 4 Cell culture medium containing 0.0005% LPS and 1% Comparative Composition 1 Sample group 5 Cell culture medium containing 0.0005% LPS and 1% control composition 2 Sample group 6 Cell culture medium containing 0.0005% LPS and 1% control composition 3 Sample group 7 Cell culture medium containing 0.0005% LPS and 1% control composition 4 Sample group 8 Cell culture medium containing 0.0005% LPS and 1% control composition 5

[0100] RAW264.7 cells in logarithmic growth phase were seeded into 24-well plates and incubated at 37°C in a 5v / v %CO2 incubator. After 24 h of cell culture, the culture medium was aspirated from the plates, and the cells were washed once with PBS. Treatments were then administered according to the groups listed in Table 3, with 6 replicates per treatment group. Cells were incubated at 37°C in a 5v / v %CO2 incubator for another 24 h, and the supernatant was collected. TNF-α and IL-6 levels were detected according to the ELISA kit instructions.

[0101] 1.4 Test Results

[0102] Table 4 Results of inflammatory factor levels

[0103] Group <![CDATA[TNF-α content / pg·mL -1 > <![CDATA[IL-6 content / ng·mL -1 > Blank control 295.1±20.2 1.21±0.15 negative control 3662.3±125.8 26.18±2.33 Positive control 1008.3±78.3aa 4.83±0.64aa Sample group 1 1347.8±112.7aa 11.62±1.21aa Sample group 2 1208.2±73.8aa 10.90±0.75aa Sample group 3 1366.6±157.6aa 11.23±0.95aa Sample group 4 2053.7±172.4bb 16.51±1.22bb Sample group 5 1871.7±122.7bb 17.03±2.16bb Sample group 6 1922.4±207.1bb 17.35±1.42bb Sample group 7 2105.8±175.6bb 18.72±2.05bb Sample group 8 1909.1±166.4bb 15.88±1.64bb

[0104] Note: In the table, "a" indicates a significant difference compared with the negative control group, P < 0.05, "aa" P < 0.01; "b" indicates a significant difference between sample groups 4-8 and sample group 2, P < 0.05, "bb" P < 0.01.

[0105] 1.5 Results Analysis

[0106] According to the experimental results, compared with the negative control, the content of inflammatory factors in sample groups 1-3 was significantly reduced (P<0.01), indicating that the skin care composition 1-3 provided by the present invention can effectively inhibit the activity of inflammatory factors, resist the damage of inflammatory factors to the skin, reduce lipid synthesis, and achieve the effect of oil control. Compared with sample group 2, the content of inflammatory factors in sample groups 4-8 was significantly higher than that in sample group 2 (P<0.01), proving that when any component in the skin care composition is missing, it will affect the overall effect of the formula. Therefore, the Astragalus membranaceus root extract, raspberry ketone glucoside, seaweed extract, firethorn fruit extract, and Bifida ferment lysate filtrate in the skin care composition are indispensable in the formula, and they have a certain synergistic effect when used in combination.

[0107] 2COL-I expression detection

[0108] 2.1 Experimental Principle

[0109] COL-I is a major component of extracellular matrix proteins in animals, accounting for 25%–30% of total human protein. COL-I interacts with the extracellular matrix to promote cell growth, adhesion, migration, and deposition; it can induce chemotaxis in fibroblasts, promoting wound repair. COL-I is the most abundant protein in the cell matrix and provides mechanical support to surrounding tissues. Reduced COL-I synthesis is a significant factor in decreased skin elasticity, enlarged pores, and wrinkle formation.

[0110] 2.2 MTT assay for cytotoxicity

[0111] The test method is the same as in 1.2.

[0112] 2.3 COL-I Expression Detection

[0113] HFF-1 cells in logarithmic growth phase were seeded into 24-well plates and incubated at 37°C in a 5v / v % CO2 incubator for 24 h. After incubation, the culture medium was aspirated from the plates, and all cells were washed once with PBS. Cell culture medium was added to the blank control group, and cell culture medium containing 1% of the test substance (compositions 1-3 and control compositions 1-5) was added to the sample groups. Each treatment group had 6 replicates. After incubation for 48 h, the supernatant was collected. COL-I content was detected according to the ELISA kit instructions.

[0114] 2.4 Test Results

[0115] Table 5. Results of COL-I content

[0116] Grouping <![CDATA[COL-I content / ng·mL -1 > Blank control 1.61±0.13 Sample group 1 (containing 1% composition 1) 12.55±1.03aa Sample group 2 (containing 1% composition 2) 13.08±1.54aa Sample group 3 (containing 1% composition 3) 13.11±0.93aa Sample group 4 (containing 1% control composition 1) 6.33±0.82bb Sample group 5 (containing 1% control composition 2) 7.21±1.14bb Sample group 6 (containing 1% control composition 3) 5.46±0.65bb Sample group 7 (containing 1% control composition 4) 6.37±0.67bb Sample group 8 (containing 1% of control composition 5) 8.64±1.31bb

[0117] Note: In the table, "a" indicates a significant difference compared with the blank control group, P < 0.05, "aa" P < 0.01; "b" indicates a significant difference compared with sample group 2, P < 0.05, "bb" P < 0.01.

[0118] 2.5 Results Analysis

[0119] According to the experimental results in Table 5, compared with the blank control, the COL-I content of sample groups 1-3 was significantly increased (P<0.01), indicating that the skin care composition 1-3 provided by the present invention can effectively promote the synthesis and expression of COL-I in skin cells, improve skin firmness, effectively resist wrinkles and aging, and reduce enlarged pores. Compared with sample group 2, the promoting effect of sample groups 4-8 was significantly less than that of sample group 2, indicating that when any component in the skin care composition is missing, it will affect the overall firming effect of the formula. Therefore, the Astragalus membranaceus root extract, raspberry ketone glucoside, seaweed extract, firethorn fruit extract, and Bifida ferment lysate filtrate in the skin care composition are indispensable in the formula, and they have a certain synergistic effect when used in combination.

[0120] 3. Human efficacy test

[0121] 3.1 Subject selection

[0122] Thirty male subjects aged 30-35 with similar skin conditions were selected for pore shrinkage and skin elasticity tests.

[0123] 3.2 Preparation of test samples

[0124] Weigh the raw materials precisely according to the mass percentage in Table 6;

[0125] Step 1. Heat deionized water to 85°C, then add sucrose stearate, glyceryl stearate, caprylic / capric triglyceride and polydimethylsiloxane in sequence. Stir well at 200 r / min and keep warm for 20 min to obtain mixture A.

[0126] Step 2. Cool mixture A to 50°C, add glycerol, butanediol and phenoxyethanol in sequence, and stir at 200 r / min until homogeneous to obtain mixture B;

[0127] Step 3. Cool mixture B to 35°C, add skin care composition 2, stir evenly at 200 r / min, add an appropriate amount of pH adjuster, and obtain the test sample of the essence.

[0128] Table 6. Ingredients (%) of the serum

[0129] Raw material name Serum 1 Serum 2 matrix solution Skincare Composition 2 5 10 / glycerin 3 3 3 Butylene glycol 2 2 2 Sucrose stearate 1 1 1 Glyceryl stearate 1 1 1 Caprylic / Capric Triglycerides 1 1 1 polydimethylsiloxane 1 1 1 Phenoxyethanol 0.2 0.2 0.2 Deionized water Add to 100 Add to 100 Add to 100

[0130] 3.3 Test Plan

[0131] Thirty subjects were randomly divided into three groups of 10 each. Each group used serum 1, serum 2, and base solution respectively for a comparative experiment. Subjects applied 2g of serum / base solution to their face twice a day, morning and evening, for 30 consecutive days.

[0132] Pore ​​detection: Before using the sample and 30 days after use, skin pore data were measured using the Antera3D skin imaging analyzer. The data before and after use were compared to evaluate the pore-shrinking effect of the test sample. The average value of the data was taken.

[0133] Skin elasticity test: Before using the sample and 30 days after use, the skin R2 value was measured using a Cutometer MPA580. The closer R2 is to 1, the better the skin elasticity. By comparing the data before and after use, the effect of the test sample on skin firming and anti-wrinkle was evaluated, and the average value of the data was taken.

[0134] 3.4 Test Results

[0135] Table 7 Test Results

[0136]

[0137]

[0138] 3.5 Results Analysis

[0139] The results show that, compared to the base liquid, serums 1 and 2 can effectively tighten pores, improve skin firmness, and increase skin elasticity. Serum 2 is more effective than serum 1, proving that the oil-controlling, skin-tightening, and anti-wrinkle effects of the serums increase with the concentration of the skincare composition, showing a certain concentration dependence. This indicates that the skincare composition has excellent oil-controlling, pore-tightening, and anti-wrinkle effects.

[0140] In summary, the skincare composition and serum containing the composition provided by this invention have excellent astringent, oil-controlling, anti-wrinkle, and anti-aging effects. They can effectively shrink pores and improve the appearance of enlarged pores. At the same time, they can promote the synthesis of COL-I in skin cells, improve skin firmness, and reduce skin aging.

[0141] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A skin care composition characterized in that, The composition consists of the following components by mass fraction: Membrane Astragalus root extract 5-8 parts; Raspberry ketoglycoside 0.15-0.2 parts; Sea moss extract 3-5 parts; Fireberry fruit extract 0.8-1 part; Fermentation product filtrate of bifida yeast 20-30 parts; The preparation method of the fireberry fruit extract comprises the following steps: Step 3-1. A certain amount of dried fireberry fruit is crushed in a pulverizer to pass through a 60-mesh sieve to obtain fireberry powder. The fireberry powder is moistened with deionized water to achieve a water content of 40-50%, thereby obtaining a solid-state fermentation substrate. The substrate is sterilized and prepared for use; Step 3-2. The viable cell count is ≥1×10 8 CFU / mL of the Candida versatilis bacterial solution, the bacterial solution is inoculated into the solid-state fermentation substrate in the form of spraying at 15-20% of the fermentation substrate mass, and is cultured at 28-30°C, an aeration amount of 0.5-0.7 L / min·kg, and a stirring paddle rotating speed of 3-7 r / min for 72-80 h to obtain the fermentation material. Step 3-3. The fermentation material is mixed with 70-80v / v% ethanol aqueous solution at a ratio of 1:15-20g / mL. Reflux extraction is performed at 70-80℃ for 3 times, each time for 1-1.2h. The filtrate is combined and concentrated under reduced pressure to 1 / 3 of the original volume. Finally, vacuum drying is performed to achieve a water content of ≤10%, thereby obtaining the fireberry fruit extract; The preservation number of the strange yeast is CGMCC 2.5692, which is purchased from the China General Microbiological Culture Collection Center.

2. The skin care composition of claim 1, wherein The preparation method of the membrane Astragalus root extract comprises the following steps: Step 1-1. Dry membrane Astragalus root is crushed in a pulverizer to pass through a 30-mesh sieve to obtain membrane Astragalus root powder; Step 1-2. The membrane Astragalus root powder is mixed with deionized water at a ratio of 1:20-30g / mL. Ultrasonic treatment is performed at a frequency of 25-30kHz and a temperature of 50-60℃ for 20-30min. After ultrasonic treatment, the mixture is continuously soaked at 50-60℃ for 12-16h. The mixture is then sieved through a 300-400-mesh sieve to obtain a crude extract; Step 1-3. The crude extract is concentrated under reduced pressure in a concentration device at 30-50kPa and 60-70℃ to 1 / 3 of the original volume, thereby obtaining the membrane Astragalus root extract.

3. The skin care composition of claim 1, wherein The preparation method of the sea moss extract comprises the following steps: Step 2-1. A certain amount of sea moss is mixed with deionized water at a ratio of 1:20-30g / mL. Homogenization is performed at 20000-30000r / min for 10-15min to obtain sea moss homogenate; Step 2-2. The sea moss homogenate is heated to 70-80℃ and maintained for 50-60min. The temperature is then reduced to 25-35℃ and centrifuged to obtain supernatant. 95v / v% ethanol aqueous solution is added to the supernatant and mixed for 24-26h. Centrifugation is performed to obtain precipitate; Step 2-3. The precipitate obtained in step 2-2 is dried in a 50-60℃ oven until there is no alcohol smell, thereby obtaining the sea moss extract.

4. Use of the skin care composition of any one of claims 1-3 in the preparation of a skin care product with anti-wrinkle, firming, and oil control effects.

5. An essence having a pore-shrinking, skin-smoothing, and wrinkle-removing effect, characterized by comprising the compound of claim 1. The serum contains the skin care composition of any one of claims 1-3; The addition amount of the skin care composition in the serum is 5-10wt%.

6. The serum of claim 5, wherein, The serum further contains a preservative, an antioxidant, a humectant, and a solvent.

7. The method of claim 6, wherein the serum is prepared by the steps of: The preparation method comprises the following steps: The skin care composition, preservative, antioxidant, humectant and solvent are mixed and stirred uniformly to obtain the essence.

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