Nano-scale repairing composition, nano-scale repairing cosmetic and preparation method and application of nano-scale repairing composition and nano-scale repairing cosmetic

Abstract

The invention relates to the technical field of cosmetics, in particular to a nanoscale repair composition, a nanoscale repair cosmetic and a preparation method and application of the nanoscale repair composition and the nanoscale repair cosmetic. The invention discloses a nanoscale repairing composition. The traditional Chinese medicine composition is prepared from the following raw material components in parts by weight: 4 to 6 parts of herba centellae extract, 2 to 4 parts of chamomile extract, 2 to 4 parts of radix ginseng extract, 1 to 3 parts of rhizoma curcumae longae extract, 3 to 5 parts of aloe extract, 1 to 3 parts of oat extract, 1 to 2 parts of liquorice root extract, 0.3 to 0.7 part of green tea extract, 0.5 to 1.5 parts of radix salviae miltiorrhizae extract, 0.5 to 1.5 parts of radix angelicae sinensis extract and 0.5 to 1.5 parts of ganoderma atrum extract. , 0.5 to 1.5 parts of radix astragali seu hedysari extract, 0.3 to 0.7 part of rhizoma polygoni cuspidati extract, and 0.3 to 0.7 part of onion extract. The nanoscale repairing composition and the nanoscale repairing cosmetic disclosed by the invention can improve the moisture content of skin, reduce moisture loss and redness of the skin, accelerate healing of injured skin, effectively relieve inflammatory response and promote collagen regeneration of the skin.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a nano-scale repairing composition, a nano-scale repairing cosmetic, and a preparation method and application thereof. Background Art

[0002] Skin damage is a complex, multi-factorial process. Depending on the cause of the damage, it can be divided into endogenous damage and exogenous damage. Among them, resisting exogenous damage is the main way to repair skin care products. Exogenous damage refers to skin damage caused by external factors such as ultraviolet radiation, environmental pollution, mechanical friction, and contact with toxic and harmful chemicals, and ultraviolet radiation is the most important factor causing exogenous skin damage. The causes and mechanisms of skin damage are very complex. In addition to age, ultraviolet radiation, staying up late, mental stress, emotional instability, excessive fatigue, etc. will promote skin damage, which is mainly manifested in decreased skin barrier function, increased water loss, and inflammatory reactions such as redness, itching, and peeling. The main causes of skin damage are impaired barrier function, increased inflammatory response, and free radical oxidative attack. Therefore, one of the current research hotspots is to develop skin care products that repair damaged skin.

[0003] For repairing cosmetics, plant-derived repairing ingredients are gaining greater favor in both consumer perception and research. They also demonstrate superior results in stimulation and safety testing during the development of repairing cosmetics. Consequently, an increasing number of repairing ingredients have been researched and reported in recent years.

[0004] Chinese patent CN109363991A discloses a skin care composition containing anti-allergic plant extracts, comprising the following components by weight: 0.01-30% of an anti-allergic plant extract combination; 10-40% oil; 1-20% of an emulsifier; 5-20% of a co-emulsifier; 0.1-5% of a thickener; and the balance being pure water. The anti-allergic plant extract combination includes extracts of Centella asiatica, licorice, chamomile, green tea, and knotweed root. This composition can reduce transepidermal water loss in patients with dermatitis symptoms, with a maximum change rate of 81.19%. However, its repair, anti-inflammatory, and collagen synthesis-promoting abilities are relatively poor.

[0005] Wang Shuo et al. studied the effects of varying aloe vera powder and oat extract content on the moisturizing properties of gel-based skincare cosmetics in a study evaluating the moisturizing properties of gel-based skincare cosmetics containing plant-based ingredients. A gel formula was used to create four gel samples (no added ingredients; 0.3% aloe vera powder and 2% oat extract; 0.6% aloe vera powder and 2% oat extract; and 0.6% aloe vera powder) with varying aloe vera powder and oat extract content. Thirty healthy subjects (15 males and 15 females) were selected and evaluated using a simple method for evaluating the moisturizing properties of human skin, specifically for cosmetic raw materials and finished products. The hydration state and water loss values of the inner forearms of the subjects were measured. The rate of change in water state and water loss values during the experiment was calculated to characterize the moisturizing properties of the cosmetics during the testing period. Data for the four gel samples were plotted and statistically analyzed. The results showed that gel-based cosmetics with 0.6% and 2% aloe vera powder and oat extract, respectively, exhibited superior moisturizing properties. However, no research has been conducted on skin repair. Summary of the Invention

[0006] The present invention provides a nanoscale repair composition, a nanoscale repair cosmetic, and a preparation method and application thereof. The nanoscale repair composition and nanoscale repair cosmetic of the present invention can increase the moisture content of the skin, reduce skin moisture loss and redness, and at the same time can accelerate the healing of damaged skin, effectively relieve inflammatory reactions and promote skin collagen regeneration.

[0007] To achieve the above object, the technical solution adopted by the present invention is as follows:

[0008] In a first aspect, the present invention provides a nanoscale repair composition, comprising the following raw material components by weight: 4-6 parts of Centella asiatica extract, 2-4 parts of chamomile extract, 2-4 parts of ginseng extract, 1-3 parts of turmeric extract, 3-5 parts of aloe extract, 1-3 parts of oat extract, 1-2 parts of licorice extract, 0.3-0.7 parts of green tea extract, 0.5-1.5 parts of salvia miltiorrhiza extract, 0.5-1.5 parts of angelica extract, 0.5-1.5 parts of black ganoderma extract, 0.5-1.5 parts of astragalus extract, 0.3-0.7 parts of knotweed extract, and 0.3-0.7 parts of onion extract.

[0009] Preferably, the nano-scale repair composition comprises the following raw material components by weight: 4-5 parts of Centella asiatica extract, 2-3 parts of chamomile extract, 3-4 parts of ginseng extract, 2-3 parts of turmeric extract, 3-4 parts of aloe extract, 2 parts of oat extract, 2-3 parts of licorice extract, 0.3-0.5 parts of green tea extract, 1 part of salvia miltiorrhiza extract, 1 part of angelica extract, 1 part of black ganoderma extract, 1 part of astragalus extract, 0.5 part of knotweed extract, and 0.5 part of onion extract.

[0010] More preferably, the nano-scale repair composition comprises the following raw material components in parts by weight: 5 parts of Centella asiatica extract, 3 parts of chamomile extract, 3 parts of ginseng extract, 2 parts of turmeric extract, 4 parts of aloe extract, 2 parts of oat extract, 2 parts of licorice extract, 0.5 parts of green tea extract, 1 part of salvia miltiorrhiza extract, 1 part of angelica extract, 1 part of black ganoderma extract, 1 part of astragalus extract, 0.5 parts of knotweed extract, and 0.5 parts of onion extract.

[0011] In a second aspect, the present invention provides a nano-scale repair cosmetic comprising the nano-scale repair composition.

[0012] Furthermore, the nano-scale repair cosmetics also include ceramide, moisturizer, emollient, thickener, emulsifier, preservative and water.

[0013] Furthermore, the nano-scale repair cosmetic comprises the following raw material components by weight: 24-30 parts of nano-scale repair composition, 1-3 parts of ceramide, 9-14 parts of moisturizer, 9-15 parts of emollient, 0.3-0.7 parts of thickener, 2-4 parts of emulsifier, 0.8-1.2 parts of preservative, and water added to 100 parts.

[0014] Preferably, the water is deionized water.

[0015] Furthermore, the moisturizer includes at least one of glycerin, sodium hyaluronate and panthenol; the emollient includes at least one of squalane, shea butter, jojoba oil, sea buckthorn fruit oil and isononyl isononanoate; the thickener includes xanthan gum, carrageenan and hydroxyethyl cellulose; the emulsifier includes at least one of olive oil emulsifying wax, cetearyl glucoside and polyglyceryl-4 oleate; the preservative includes at least one of phenoxyethanol and ethylhexylglycerin.

[0016] Preferably, the sodium hyaluronate is low molecular weight sodium hyaluronate.

[0017] Preferably, the nano-scale repair cosmetic further comprises a pH regulator.

[0018] More preferably, the pH adjuster is at least one of citric acid and sodium citrate.

[0019] As a preferred embodiment, the nano-scale repair cosmetic comprises the following raw materials in parts by weight: 5 parts of Centella asiatica extract, 3 parts of chamomile extract, 3 parts of ginseng extract, 2 parts of turmeric extract, 4 parts of aloe extract, 2 parts of oat extract, 2 parts of licorice extract, 0.5 parts of green tea extract, 1 part of salvia miltiorrhiza extract, 1 part of angelica extract, 1 part of black ganoderma extract, 1 part of astragalus extract, 0.5 parts of knotweed extract, 0.5 parts of onion extract, 2 parts of ceramide NP, 8 parts of glycerin, 1.5 parts of sodium hyaluronate, 2 parts of panthenol (vitamin B5), 4 parts of squalane, 5 parts of shea butter, 3 parts of jojoba oil, 3 parts of olive oil emulsifying wax, 0.5 parts of xanthan gum, 1 part of phenoxyethanol + ethylhexylglycerin, 43.5 parts of deionized water, and an appropriate amount of citric acid / sodium citrate to adjust the pH to 5.5-6.0.

[0020] In a third aspect, the present invention provides the use of the nano-scale repair cosmetics in repairing skin barriers and resisting inflammatory reactions.

[0021] Preferably, the skin barrier repair is to increase the moisture content of the skin, reduce skin moisture loss and redness, and at the same time accelerate the healing of damaged skin and promote skin collagen regeneration.

[0022] In a fourth aspect, the present invention provides a method for preparing the nanoscale repair cosmetic, comprising the following steps:

[0023] (1) Preparation of aqueous phase: Add glycerin and sodium hyaluronate to water, stir until completely dissolved, add the nanoscale repair composition according to any one of claims 1 to 2, add a thickener, and stir until no particles are present;

[0024] (2) Preparation of oil phase: Heat and stir the emollient and emulsifier until completely melted and mixed, add ceramide, and stir;

[0025] (3) Emulsification and mixing: slowly pour the oil phase into the water phase and homogenize and emulsify to obtain an emulsion;

[0026] (4) Cooling and post-treatment: Cool the emulsion, add panthenol and preservatives, mix well, adjust the pH to 5.5-6.0, and filter sterilize.

[0027] Preferably, the temperature of the water is 40-45°C.

[0028] Preferably, the stirring is homogeneous stirring.

[0029] Preferably, the heating temperature in step (2) is 50-55°C.

[0030] Preferably, the flow rate of the slow pouring in step (3) is 3-6 mL / min.

[0031] Preferably, the homogenization and emulsification in step (3) is first performed at 2000-3000 rpm for 5-10 minutes, and then a high-pressure homogenizer (10000 psi, 2 cycles) is used to further refine the emulsion.

[0032] Preferably, the filtration is through a 0.22 μm microporous filter membrane.

[0033] Skin damage is mainly caused by external stimuli (such as ultraviolet rays, pollutants, mechanical damage) and internal factors (such as inflammation and oxidative stress). Its core mechanisms include: barrier function destruction (disordered lipid structure of the stratum corneum, increased transepidermal water loss); inflammatory cascade reaction (activation of the NF-κB pathway, release of pro-inflammatory factors such as IL-6 and TNF-α); extracellular matrix degradation (overexpression of MMPs enzymes, loss of collagen, and delayed tissue repair).

[0034] The selection of raw materials lies in constructing a multi-target nano-scale repair composition that covers the entire pathway of "barrier repair-anti-inflammation-promoting regeneration", focusing on solving the problems of barrier function recovery, inflammation relief and tissue regeneration after skin damage.

[0035] The raw material effects of the present invention are:

[0036] Centella asiatica extract contains asiaticoside, which can directly activate the TGF-β / Smad pathway, stimulate type I / III collagen synthesis, and repair UV-induced DNA breaks. The core effect of asiaticoside is that it directly stimulates collagen and fibroblast regeneration, repairing scars and damage.

[0037] Chamomile extract contains bisabolol and apigenin, which can directly inhibit the NF-κB pathway, reducing the release of inflammatory factors such as IL-6 and TNF-α. It can also activate EGF receptor signaling, promoting keratinocyte migration and differentiation. The core effects of bisabolol are anti-inflammatory and soothing, while apigenin promotes epidermal regeneration and accelerates wound healing.

[0038] Ginseng extract contains ginsenosides (Rg3 and Rb1), which can directly inhibit the NF-κB pathway and reduce the release of inflammatory factors. They can also activate the SIRT1 gene and delay cellular aging. Ginsenosides' core benefits are their antioxidant and anti-inflammatory properties, promoting epidermal stem cell proliferation and supporting post-operative repair.

[0039] Turmeric extract contains curcumin, which can directly inhibit the COX-2 / PGE2 inflammatory pathway, reducing erythema reactions. It can also scavenge free radicals and protect cells from oxidative damage. Curcumin's core anti-inflammatory and antioxidant properties can alleviate postoperative inflammation and oxidative stress.

[0040] Aloe vera extract contains aloe polysaccharides, which can directly form a physical moisturizing film to reduce transepidermal water loss (TEWL). It can also inhibit the release of IL-1β and alleviate postoperative redness and swelling. The core function of aloe polysaccharides is to moisturize and repair, and strengthen the skin barrier function.

[0041] Oat extract contains β-glucan, which can directly enhance the expression of tight junction protein (Claudin-1) and repair the physical barrier. It can also promote fibroblast proliferation and support collagen synthesis. The core function of β-glucan is to repair the barrier and relieve postoperative skin sensitivity.

[0042] Licorice extract contains dipotassium glycyrrhizate, which can directly block MAPK / AP-1 signaling and reduce MMP production. It can also stabilize mast cells and prevent postoperative allergic reactions. Its core function is to combat inflammation and allergies, stabilizing the repair environment.

[0043] Green tea extract contains EGCG, which can directly scavenge free radicals and inhibit lipid peroxidation. It can also inhibit the risk of photocarcinogenesis through the p53 pathway. EGCG's core antioxidant function protects postoperative skin from oxidative damage.

[0044] Danshen extract contains tanshinone IIA, which can directly inhibit the expression of MMP-1 / 3 and protect collagen. It can also improve microcirculation and repair endothelial function after surgery. The core function of tanshinone IIA is to promote microcirculation and support tissue regeneration.

[0045] Angelica extract contains ferulic acid, which can directly inhibit tyrosinase activity and reduce pigmentation. It can also prolong cell proliferation through the PI3K / Akt pathway. The core function of ferulic acid is its antioxidant properties, promoting even skin repair after surgery.

[0046] Black Ganoderma extract contains Ganoderma triterpenes, which can directly regulate the Th1 / Th2 immune balance, inhibit chronic inflammation, and promote keratinocyte migration and repair. The core function of Ganoderma triterpenes is immune regulation, supporting postoperative skin barrier reconstruction.

[0047] Astragalus extract contains astragaloside IV, which can directly activate the TLR4 receptor in fibroblasts, promoting collagen secretion and enhancing the activity of SOD / GSH-Px antioxidant enzymes. Its core function is to promote regeneration and improve postoperative skin elasticity.

[0048] Polygonum cuspidatum extract contains resveratrol, which can directly inhibit bacterial growth and prevent wound infection. It can also scavenge free radicals and reduce oxidative stress damage. The core function of resveratrol is its antibacterial and antioxidant properties, supporting postoperative wound cleansing and repair.

[0049] Onion extract contains quercetin, which can directly inhibit the release of inflammatory factors and reduce postoperative redness and swelling. It can also scavenge free radicals and protect cells from oxidative damage. The core effect of quercetin is its anti-inflammatory and antioxidant properties, promoting rapid skin repair after surgery.

[0050] Synergy between raw materials:

[0051] (1) Barrier repair and moisturizing: Aloe vera moisturizes through polysaccharides, and oatmeal strengthens tight junctions through β-glucan, synergistically repairing the physical barrier. Ceramide replenishes lipids, and squalane simulates sebum, synergistically repairing the lipid barrier.

[0052] (2) Anti-inflammatory: Ginseng inhibits NF-κB and turmeric blocks COX-2, forming a dual anti-inflammatory network that covers key nodes of the inflammatory cascade. Chamomile soothes mast cells, and licorice stabilizes the repair environment, synergistically reducing the risk of postoperative allergies and irritation.

[0053] (3) Promote regeneration: Centella asiatica stimulates collagen synthesis through TGF-β, while chamomile promotes epidermal regeneration through EGF, covering both dermis and epidermis for double repair. Astragalus promotes fibroblast proliferation, while Angelica sinensis prolongs the cell proliferation cycle, synergistically enhancing regeneration efficiency.

[0054] (4) Antioxidant: Green tea directly scavenges free radicals, while turmeric activates endogenous antioxidant enzymes, forming a dual antioxidant network. Green tea and onion quercetin synergistically scavenge ROS, covering both water-soluble and fat-soluble antioxidant pathways.

[0055] Compared with the prior art, the present invention has the following beneficial effects:

[0056] The active ingredients contained in the nano-scale repair cosmetics of the present invention have the advantages of multi-target repair and comprehensive efficacy. The active ingredients in the formula (such as Centella asiatica, chamomile, ginseng, turmeric, etc.) work synergistically, covering the three core repair pathways of "barrier repair-anti-inflammatory-promoting regeneration", and comprehensively solving the problem of skin damage. Centella asiatica extract directly stimulates the regeneration of collagen and fibroblasts by activating the TGF-β / Smad pathway, repairing scars and damage; chamomile extract significantly resists inflammation and promotes epidermal regeneration by inhibiting the NF-κB pathway and activating the EGF receptor signaling pathway; ginseng extract reduces the release of inflammatory factors by inhibiting the NF-κB pathway, while anti-oxidation delays cell aging; turmeric extract reduces postoperative redness and swelling and oxidative stress damage by blocking the COX-2 / PGE2 inflammatory pathway and scavenging free radicals. In addition, aloe vera and oat extracts synergistically repair the skin's physical barrier through polysaccharide moisturizing and enhancing the expression of tight junction proteins; green tea extract and onion extract further resist oxidation and inflammation by scavenging free radicals and inhibiting the release of inflammatory factors. The active ingredients contained in the repair cosmetics of the present invention are all natural plant ingredients, safe and gentle, suitable for use on sensitive skin, and meet the current consumer pursuit of pure beauty. Through nano-technology (such as liposome encapsulation and nanocrystal processing), the active ingredients can efficiently penetrate into the dermis, significantly improving the repair effect. The nano-scale repair cosmetics of the present invention have a wide range of use scenarios, and are applicable to post-medical cosmetic repair, sensitive skin barrier reconstruction, and daily protection, and have significant market competitiveness and application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0057] Figure 1 VISIA red zone images of the face (right side) of Subject 1 using the composition prepared in Example 1 before use on the first day and after use on day 28.

[0058] Figure 2 VISIA red zone images of the face (front) of Subject 1 using the composition prepared in Example 1 before use on the first day and after use for 28 days.

[0059] Figure 3 VISIA red zone images of the face (left side) of Subject 1 using the composition prepared in Example 1 before the first day of use and after 28 days of use.

[0060] Figure 4 VISIA red zone images of the face (right side) of subject 2 using the composition prepared in Example 1 before use on the first day and after use on day 28.

[0061] Figure 5 VISIA red zone images of the face (front) of Subject 2 using the composition prepared in Example 1 before use on the first day and after use on day 28.

[0062] Figure 6 VISIA red zone images of the face (left side) of Subject 2 using the composition prepared in Example 1 before the first day of use and after 28 days of use.

[0063] Figure 7 VISIA red zone images of the face (right side) of subject 3 using the composition prepared in Example 1 before use on the first day and after use on day 28.

[0064] Figure 8 VISIA red zone images of the face (front) of Subject 3 using the composition prepared in Example 1 before use on the first day and after use on day 28.

[0065] Figure 9 VISIA red zone images of the face (left side) of subject 3 using the composition prepared in Example 1 before the first day of use and after 28 days of use.

[0066] Figure 10 This is a picture of the tail fin regeneration phenotype of the negative control group taken 72 hours after tail amputation in the zebrafish tail fin regeneration experiment of the present invention.

[0067] Figure 11 This is a picture of the tail fin regeneration phenotype of a 3 dpf healthy zebrafish taken 72 hours after tail amputation in the zebrafish tail fin regeneration experiment of the present invention, using the method of Example 1.

[0068] Figure 12 This is a picture of the tail fin regeneration phenotype of a 3 dpf healthy zebrafish in the zebrafish tail fin regeneration experiment of the present invention, taken 72 hours after tail amputation using the TGF-β group. DETAILED DESCRIPTION

[0069] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only some embodiments of the present invention, rather than all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work are within the scope of protection of the present invention. The materials, reagents, etc. used, unless otherwise specified, are reagents and materials available from commercial sources.

[0070] Source of raw materials: Centella asiatica extract: purchased from Guangzhou Banmi Biotechnology Co., Ltd.; Chamomile extract: purchased from Guangzhou Banmi Biotechnology Co., Ltd.; Aloe vera extract: purchased from Guangzhou Banmi Biotechnology Co., Ltd.; Oat extract: purchased from Guangzhou Banmi Biotechnology Co., Ltd.; Ginseng extract: purchased from Guangzhou Banmi Biotechnology Co., Ltd.; Turmeric extract: purchased from Guangzhou Banmi Biotechnology Co., Ltd.; Licorice extract: purchased from Guangzhou Banmi Biotechnology Co., Ltd.; Green tea extract: purchased from Guangzhou Banmi Biotechnology Co., Ltd.; Salvia miltiorrhiza extract: purchased from Guangzhou Banmi Biotechnology Co., Ltd.; Angelica sinensis extract: purchased from Guangzhou Banmi Biotechnology Co., Ltd.; Black Ganoderma lucidum extract: purchased from Guangzhou Banmi Biotechnology Co., Ltd.; Astragalus extract: purchased from Guangzhou Banmi Biotechnology Co., Ltd.; Polygonum cuspidatum extract: purchased from Guangzhou Banmi Biotechnology Co., Ltd.; Onion extract: purchased from Guangzhou Banmi Biotechnology Co., Ltd.

[0071] The "ranges" disclosed herein are in the form of lower limits and upper limits. There can be one or more lower limits, and one or more upper limits, respectively. A given range is defined by selecting a lower limit and an upper limit.

[0072] The lower and upper limits define the boundaries of a particular range. All ranges that can be limited in this way are inclusive and combinable, i.e., any lower limit can be combined with any upper limit to form a range. For example, if a range of 60-120 and 80-110 is listed for a particular parameter, it is understood that a range of 60-110 and 80-120 is also expected. In addition, if the minimum range values listed are 1 and 2, and if the maximum range values listed are 3, 4, and 5, then the following ranges can all be expected: 1-2, 1-4, 1-5, 2-3, 2-4, and 2-5.

[0073] In this disclosure, unless otherwise specified, the numerical range "ab" is an abbreviation for any combination of real numbers between a and b, where a and b are real numbers. For example, the numerical range "0-5" indicates that all real numbers between "0-5" are listed herein, and "0-5" is merely an abbreviation for these numerical combinations.

[0074] In the present invention, unless otherwise specified, all embodiments and preferred embodiments mentioned herein can be combined with each other to form a new technical solution.

[0075] In the present invention, unless otherwise specified, all technical features and preferred features mentioned herein can be combined with each other to form a new technical solution.

[0076] 1. Examples and Comparative Examples

[0077] Example 1 Nanoscale repair composition

[0078] In terms of weight, it is composed of the following raw materials: 5 parts of Centella asiatica extract, 3 parts of chamomile extract, 3 parts of ginseng extract, 2 parts of turmeric extract, 4 parts of aloe extract, 2 parts of oat extract, 2 parts of licorice extract, 0.5 parts of green tea extract, 1 part of salvia miltiorrhiza extract, 1 part of angelica extract, 1 part of black ganoderma extract, 1 part of astragalus extract, 0.5 parts of knotweed extract, and 0.5 parts of onion extract.

[0079] Example 2 Nanoscale repair composition

[0080] The invention is composed of the following raw materials in parts by weight: 4 parts of Centella asiatica extract, 2 parts of chamomile extract, 4 parts of ginseng extract, 3 parts of turmeric extract, 3 parts of aloe extract, 2 parts of oat extract, 3 parts of licorice extract, 0.3 parts of green tea extract, 1 part of salvia miltiorrhiza extract, 1 part of angelica extract, 1 part of black ganoderma extract, 1 part of astragalus extract, 0.5 parts of knotweed extract and 0.5 parts of onion extract.

[0081] Example 3 Nanoscale repair composition

[0082] The invention is composed of the following raw materials in parts by weight: 6 parts of Centella asiatica extract, 4 parts of chamomile extract, 3 parts of ginseng extract, 2 parts of turmeric extract, 5 parts of aloe extract, 2 parts of oat extract, 1 part of licorice extract, 0.7 parts of green tea extract, 0.5 parts of salvia miltiorrhiza extract, 0.5 parts of angelica extract, 0.5 parts of black ganoderma extract, 0.5 parts of astragalus extract, 0.5 parts of knotweed extract and 0.5 parts of onion extract.

[0083] The nano-scale repair cosmetics of Examples 1-3 are prepared as follows:

[0084] (1) Preparation of aqueous phase: Heat deionized water to 45°C, add glycerin and sodium hyaluronate, and stir at low speed (200 rpm) until completely dissolved. Add the nano-scale plant composition in sequence, maintaining the temperature ≤ 45°C to avoid high temperature damage to the nanostructure. Add xanthan gum and stir homogenously (500 rpm, 10 minutes) until no particles are present.

[0085] (2) Oil phase preparation: Heat shea butter, jojoba oil, squalane, and olive oil emulsified wax to 55°C and stir until completely melted and mixed. Add ceramide NP and maintain low stirring speed (150 rpm) to prevent oxidation.

[0086] (3) Emulsification and mixing: The oil phase was slowly poured into the aqueous phase at a controlled flow rate (5 mL / min) and homogenized at 3000 rpm for 5 min. A high-pressure homogenizer (10,000 psi, 2 cycles) was used to further refine the emulsion to ensure uniform dispersion of the nanoparticles.

[0087] (4) Cooling and post-treatment: Cool the emulsion to 30°C, add panthenol, phenoxyethanol, and ethylhexylglycerin, and stir (200 rpm) to mix thoroughly. Adjust the pH with citric acid / sodium citrate buffer. Sterilize the emulsion by filtering through a 0.22 μm microporous membrane and fill into a light-proof vacuum pump bottle.

[0088] Among them, ceramide NP 2 parts, glycerin 8 parts, sodium hyaluronate 1.5 parts, panthenol (vitamin B5) 2 parts, squalane 4 parts, shea butter 5 parts, jojoba oil 3 parts, olive oil emulsifying wax 3 parts, xanthan gum 0.5 parts, phenoxyethanol + ethylhexylglycerin 1 part, deionized water to 100 parts, citric acid / sodium citrate to adjust the pH to 6.0.

[0089] Comparative Example 1

[0090] Compared with Example 1, the difference is that Centella asiatica and chamomile are replaced by aloe vera to verify the necessity of the core regeneration-promoting ingredient.

[0091] That is, it is composed of the following raw material components by weight: 3 parts of ginseng extract, 2 parts of turmeric extract, 12 parts of aloe extract, 2 parts of oat extract, 2 parts of licorice extract, 0.5 parts of green tea extract, 1 part of salvia miltiorrhiza extract, 1 part of angelica extract, 1 part of black ganoderma extract, 1 part of astragalus extract, 0.5 parts of knotweed extract, and 0.5 parts of onion extract.

[0092] The obtained product was prepared according to the preparation method in Example 1.

[0093] Comparative Example 2

[0094] Compared with Example 1, the difference is that ginseng and turmeric are replaced by liquorice to verify the necessity of the anti-inflammatory core ingredient.

[0095] That is, it is composed of the following raw material components by weight: 5 parts of Centella asiatica extract, 3 parts of chamomile extract, 4 parts of aloe extract, 2 parts of oat extract, 7 parts of licorice extract, 0.5 parts of green tea extract, 1 part of Salvia miltiorrhiza extract, 1 part of Angelica sinensis extract, 1 part of black Ganoderma lucidum extract, 1 part of Astragalus membranaceus extract, 0.5 parts of Polygonum cuspidatum extract, and 0.5 parts of onion extract.

[0096] The obtained product was prepared according to the preparation method in Example 1.

[0097] Comparative Example 3

[0098] Compared with Example 1, the difference is that aloe vera and oatmeal are replaced with squalane to verify the necessity of the core ingredient of barrier repair.

[0099] That is, it is composed of the following raw material components by weight: 4 parts of Centella asiatica extract, 2 parts of chamomile extract, 4 parts of ginseng extract, 3 parts of turmeric extract, 3 parts of licorice extract, 0.3 parts of green tea extract, 1 part of salvia miltiorrhiza extract, 1 part of angelica extract, 1 part of black ganoderma extract, 1 part of astragalus extract, 0.5 parts of knotweed extract, and 0.5 parts of onion extract.

[0100] The obtained product was prepared according to the preparation method in Example 1.

[0101] Comparative Example 4

[0102] Compared with Example 1, the difference is that all plant raw materials are removed and only chemical raw materials are retained to verify the synergistic effect of the plant composition.

[0103] That is, the raw material components are as follows: 3 parts of ceramide NP, 1.5 parts of sodium hyaluronate, 2 parts of panthenol (vitamin B5), 2 parts of niacinamide, 1 part of allantoin, 0.5 parts of tocopherol (vitamin E), 5 parts of squalane, 6 parts of shea butter, 4 parts of jojoba oil, 3 parts of olive oil emulsifying wax, 0.5 parts of xanthan gum, 1 part of phenoxyethanol + ethylhexylglycerin, 59 parts of deionized water, and citric acid / sodium citrate for adjusting the pH to 6.0.

[0104] The preparation method of the nano-scale repair cosmetic comprises the following steps:

[0105] (1) Preparation of aqueous phase: Heat deionized water to 75°C, dissolve glycerin, sodium hyaluronate, xanthan gum, and carbomer in sequence, and stir until completely dissolved.

[0106] (2) Preparation of oil phase: Heat shea butter, jojoba oil, squalane, caprylic / capric triglyceride, and olive oil emulsified wax to 75°C and mix well.

[0107] (3) Emulsification and cooling: The oil phase was slowly added to the water phase, emulsified using a homogenizer (5000 rpm, 5 minutes), and then cooled to 40°C before adding preservatives, ceramide, panthenol, niacinamide, allantoin, and tocopherol.

[0108] (4) pH adjustment and filling: Adjust the pH to 6.0 with citric acid / sodium citrate, sterilize through a 0.22 μm microporous filter, and fill into light-proof vacuum pump bottles.

[0109] Comparative Example 5

[0110] The formula and preparation method of Example 1 in Chinese patent CN109363991A are adopted.

[0111] 2. Clinical Testing

[0112] Test method: The emulsion prepared by the present invention can be applied directly after cleansing the face.

[0113] Test site: face.

[0114] 28 women with impaired barrier function (aged 25-45 years) were selected and divided into 7 groups, with 4 people in each group. Each group used the emulsions of Examples 1-3 and Comparative Examples 1-4 twice a day for 28 days. After that, the moisture content of the stratum corneum, transepidermal water loss value, and skin erythema index of the skin were tested to evaluate whether the test products had repair effects.

[0115] 1. Skin stratum corneum moisture content test:

[0116] Instrument: Skin Moisture Content Tester (CM825), unit: CU. The larger the test value, the higher the skin moisture content. The average test results are shown in Table 1:

[0117] Table 1

[0118] Group Before first use (au) After 28 days of use (au) Example 1 52 78 Example 2 50 74 Example 3 49 71 Comparative Example 1 50 65 Comparative Example 2 48 62 Comparative Example 3 52 68 Comparative Example 4 50 60

[0119] According to the above data, the moisture content of the skin stratum corneum of Examples 1-3 was significantly increased. The skin moisture content of Examples 1-3 increased by 50%, 48%, and 45%, respectively, and the skin moisture content of Comparative Examples 1-4 increased by 30%, 29%, 31%, and 20%, respectively. Therefore, it can be shown that the active ingredient combination in the present invention can effectively improve the moisturizing effect of the emulsion.

[0120] 2. Transepidermal water loss test:

[0121] Instrument: Skin Moisture Loss Tester (TMHex), unit: g / m 2 / h, the smaller the test value, the better the skin barrier function. The test results are shown in Table 2:

[0122] Table 2

[0123] Group Before first use After 28 days of use Example 1 18 10 Example 2 20 12 Example 3 22 13 Comparative Example 1 20 15 Comparative Example 2 22 18 Comparative Example 3 18 14 Comparative Example 4 20 17

[0124] According to the above data, the transepidermal water loss values of Examples 1-3 groups decreased significantly, and the values after 28 days of use were lower than those of the comparative group. The change rates of Examples 1-3 were 44%, 40%, and 41%, respectively, and the change rates of Comparative Examples 1-4 were 25%, 18%, 22%, and 15%, respectively. The change rate of the Example group was greater, indicating that the skin barrier function of the Example group was better.

[0125] 3. Skin erythema index test:

[0126] Instrument: Facial Skin Image Analysis System (VISIACR). The smaller the test value, the lower the degree of skin redness. The results are shown in Table 3:

[0127] Table 3

[0128] Group Before first use After 28 days of use Example 1 45 18 Example 2 46 20 Example 3 48 19 Comparative Example 1 45 30 Comparative Example 2 50 35 Comparative Example 3 45 28 Comparative Example 4 48 40

[0129] According to the above data, the skin erythema index of the example group decreased significantly after 28 days. The change rates of Examples 1-3 were 60%, 57%, and 60%, respectively. The greater the change value, the better the degree of skin improvement. The change rates of Comparative Examples 1-4 were 33%, 30%, 38%, and 17%, respectively. Therefore, the change rates of the example group were greater than those of the comparative example group. At the same time, according to the VISIACR diagram of Example 1 (see Appendix Figure 1-9 ) It can be concluded that the composition prepared by the present invention can effectively improve the redness of the skin after use, that is, it has an excellent anti-inflammatory and repairing effect.

[0130] Therefore, according to the above data, compared with the first day without use, after 28 days of use, the moisture content of the stratum corneum of the subjects' skin in the embodiment group increased by more than 45%, and there was a significant difference; the transepidermal water loss value of the subjects decreased by more than 40%, and there was a significant difference; the skin erythema index of the subjects decreased by more than 57%, and there was a significant difference; therefore, it is shown that the emulsion prepared by the present invention has repairing, soothing and moisturizing effects.

[0131] 3. In vitro experiments

[0132] 1. HaCaT cell scratch migration assay

[0133] Reagents: HaCaT cells (human immortalized keratinocytes), DMEM medium, fetal bovine serum (FBS), penicillin-streptomycin, PBS buffer, trypsin-EDTA solution.

[0134] Instruments: cell culture incubator, inverted microscope, 6-well plate, pipette and pipette tips, cell counter.

[0135] Operation steps: a. Cell culture: HaCaT cells (human immortalized keratinocytes) were cultured in DMEM medium (containing 10% FBS) until 80% confluence.

[0136] b. Scratch production: Use a 200 μL pipette tip to make uniform scratches on the monolayer of cells, and wash with PBS to remove detached cells.

[0137] c. Group treatment: Control group: 2 mL of DMEM culture medium (containing 10% FBS) was added to each well.

[0138] Example 1 group: 2 mL of culture medium containing 1 mg / mL repair composition was added to each well.

[0139] Comparative Example 4 group: 2 mL of culture medium containing 1 mg / mL chemical raw material composition was added to each well.

[0140] d. Culture and observation: Culture at 37°C and 5% CO2 for 24 hours, and observe the healing of the scratch under a microscope.

[0141] The scratch image was opened using ImageJ software, converted into an 8-bit grayscale image, and a threshold was set to distinguish the scratch area from the cell area, and the area of the scratch area was measured.

[0142] The healing rate (%) was calculated according to the following formula. The results are shown in Table 4:

[0143] Healing rate = (1-A t / A0)*100%(A t is the scratch area at t hours, A0 is the scratch area at 0 hours)

[0144] Table 4

[0145]

[0146] The above experimental data show that nano-level repair cosmetics have a significant healing ability, with the scratch healing rate reaching 85% in Group 1 of Example 1. Therefore, the emulsion can effectively promote the healing of keratinocytes and accelerate the healing of damaged skin.

[0147] 2. Anti-inflammatory effect test (LPS-induced RAW264.7 cell model)

[0148] Reagents: LPS (lipopolysaccharide), ELISA kit (IL-6), DMEM culture medium, fetal bovine serum (FBS), dexamethasone.

[0149] Instruments: CO2 incubator, microplate reader, centrifuge, clean bench, 24-well plate.

[0150] Operation steps: a. Cell culture: RAW264.7 cells were cultured in DMEM medium containing 10% FBS (37° C., 5% CO 2 ).

[0151] b. Set up the reaction system: Normal group: no LPS and no sample added.

[0152] Control group (LPS): only LPS was added without sample.

[0153] Dexamethasone group: LPS+10 μM dexamethasone.

[0154] Example 1 group: LPS+1 mg / mL repair composition.

[0155] Comparative Example 4: LPS + 1 mg / mL chemical raw material composition.

[0156] c. Cell stimulation and collection: Pre-treat cells for 1 hour, then add LPS (1 μg / mL) for 24 hours. Collect the supernatant and centrifuge (12,000 rpm, 10 minutes) to remove cell debris.

[0157] d. ELISA test: Follow the kit instructions to determine the IL-6 concentration.

[0158] The inflammatory factor inhibition rate (%) was calculated according to the following formula. The results are shown in Table 5:

[0159] Inhibition rate = [1-(C 样品 -C 正常 ) / (C LPS -C 正常 )]*100

[0160] Table 5

[0161]

[0162] The above experimental data show that the nano-scale repair cosmetic significantly inhibits the release of inflammatory factors. The IL-6 inhibition rate of Example 1 exceeded 70%, which is comparable to that of dexamethasone. Therefore, the nano-scale repair cosmetic can effectively alleviate inflammatory reactions.

[0163] 3. Collagen synthesis promotion test (HDF fibroblast model)

[0164] Reagents: human dermal fibroblasts HDF, TGF-β, RIPA lysis buffer, BCA protein quantification kit, SDS-PAGE gel, collagen antibody (COL1A1).

[0165] Instruments: Western blot system (such as Bio-Rad), centrifuge, shaker, chemiluminescence imager.

[0166] Procedure: a. Cell culture: HDF cells were seeded in 6-well plates, 2 mL of DMEM medium (containing 10% FBS) was added to each well, and the cell density was 5×10 5 cells / mL. Culture in a 37°C, 5% CO2 incubator until the cell confluence reaches 80-90%.

[0167] b. Group treatment: Control group: 2 mL of DMEM culture medium (containing 10% FBS) was added to each well.

[0168] TGF-β group: 2 mL of culture medium containing 10 ng / mL TGF-β was added to each well.

[0169] Example 1 group: 2 mL of culture medium containing 1 mg / mL repair composition was added to each well.

[0170] Comparative Example 4 group: 2 mL of culture medium containing 1 mg / mL chemical raw material composition was added to each well.

[0171] c. Protein Extraction: Incubate the 6-well plate in a 37°C, 5% CO2 incubator for 48 hours. Discard the culture medium and wash the cells twice with PBS. Add 100 μL of RIPA lysis buffer (containing protease inhibitors) to each well and lyse the cells on ice for 30 minutes. Collect the lysate and centrifuge it at 12,000 rpm for 15 minutes at 4°C. Measure the protein concentration of the supernatant using the BCA assay.

[0172] d. Western blot: 30 μg of protein was loaded and electrophoresed on SDS-PAGE (80 V for 30 minutes, 120 V for 60 minutes). The membrane was transferred to a PVDF membrane (300 mA for 90 minutes) and blocked with 5% skim milk for 1 hour. The primary antibody (COL1A1, 1:1000) was incubated at 4°C overnight, followed by a secondary antibody (HRP-conjugated, 1:5000) at room temperature for 1 hour. ECL development was performed, and the grayscale values of the bands were quantified using ImageJ software.

[0173] The collagen expression level (relative value) was calculated according to the following formula. The results are shown in Table 6:

[0174] Relative expression level = gray value of sample group / gray value of control group

[0175] Table 6

[0176]

[0177] Based on the above experimental data, the nano-scale repair cosmetic significantly promotes collagen synthesis. Example 1 increased COL1A1 expression by 2.5 times, approaching that of the TGF-β group (3.0 times). The effect of Comparative Example 4 was significantly lower than that of the repair composition (1.2 times). Therefore, this nano-scale repair cosmetic can effectively promote skin collagen regeneration and improve skin elasticity and barrier function.

[0178] 4. Skin barrier-related protein content test (HaCaT cell model)

[0179] Reagents: HaCaT cells, DMEM medium, fetal bovine serum (FBS), penicillin-streptomycin, TRIzol reagent, reverse transcription kit, qPCR kit, primers.

[0180] Instruments: cell culture incubator, qPCR instrument, centrifuge, 6-well plate, pipette and tip, clean bench.

[0181] Procedure: a. Cell culture: HaCaT cells were seeded in a 6-well plate, and 2 mL of DMEM medium (containing 10% FBS) was added to each well. The cell density was 5×10 5cells / mL. Culture in a 37°C, 5% CO2 incubator until the cell confluence reaches 80-90%.

[0182] b. Group treatment: Control group: 2 mL of DMEM culture medium (containing 10% FBS) was added to each well.

[0183] Example 1 group: 2 mL of culture medium containing 1 mg / mL repair composition was added to each well.

[0184] Comparative Example 4 group: 2 mL of culture medium containing 1 mg / mL chemical raw material composition was added to each well.

[0185] c. RNA Extraction: Incubate the 6-well plate in a 37°C, 5% CO2 incubator for 48 hours. Discard the culture medium and wash the cells twice with PBS. Add 1 mL of TRIzol reagent to each well to lyse the cells and extract total RNA. Purify the RNA according to the TRIzol manufacturer's instructions and determine its concentration and purity (A260 / A280 ratio should be 1.8-2.0).

[0186] d. Reverse transcription and qPCR detection:

[0187] (1) cDNA synthesis: 1 μg of RNA was reverse transcribed into cDNA using a reverse transcription kit.

[0188] (2) qPCR reaction system: Reaction system (20 μL):

[0189] SYBR Green Master Mix: 10 μL

[0190] Upstream primer (10 μM): 1 μL

[0191] Downstream primer (10 μM): 1 μL

[0192] cDNA template: 2 μL

[0193] ddH2O: 6 μL

[0194] (3) qPCR procedure:

[0195] Pre-denaturation: 95℃ for 5 minutes

[0196] Cycling reaction (40 cycles): 95°C for 10 seconds; 60°C for 30 seconds

[0197] Melting curve analysis: 60℃ to 95℃, increase the reading by 1 every 0.5℃.

[0198] The Ct values of Filaggrin, Claudin-1, and GAPDH were obtained using qPCR instrument software. The relative expression levels were calculated using the following formulas. The results are shown in Table 7:

[0199] ΔCt=Ct 目标基因 -Ct GAPDH

[0200] ΔΔCt=ΔCt 样品 -ΔCt 对照组

[0201] Relative expression level = 2 -ΔΔCt

[0202] Table 7

[0203]

[0204] According to the above experimental data, Example 1 increased the expression of Filaggrin and Claudin-1 by 3.2 times and 2.8 times, respectively, which were significantly higher than those of Comparative Example 4 (1.5 times and 1.3 times). Therefore, this nano-scale repair cosmetic can effectively promote skin barrier repair.

[0205] 4. Zebrafish Experiment

[0206] 1. Zebrafish Embryo Toxicity Screening

[0207] Zebrafish: wild-type AB strain

[0208] Embryo source: obtained through natural mating, and normal development of 6hpf (hours post fertilization) embryos were selected.

[0209] Steps:

[0210] a. Exposure treatment: 6 hpf zebrafish embryos were randomly assigned to 24-well plates, with 10 embryos per well. 2 mL of the repair composition solution at different concentrations (1000, 500, 250, 125, 62.5, 31.25, and 15.625 μg / mL) was added, with three replicate wells per group.

[0211] Control group: E3 culture medium.

[0212] b. Observation indicators:

[0213] Mortality rate: The number of dead embryos (no heartbeat or whole body white) was recorded daily.

[0214] Hatching rate: The proportion of embryos hatched at 48 hpf was counted.

[0215] Deformity rate: Deformity phenotypes such as pericardial edema and spinal curvature were observed at 72 hpf.

[0216] The toxicity initial screening data (72 hpf) were calculated according to the following formula. The results are shown in Table 8:

[0217] Mortality rate = number of deaths / total number × 100%

[0218] Hatching rate = number of hatches / total number × 100%

[0219] Deformity rate = number of deformities / number of survivors × 100%

[0220] Table 8

[0221] Concentration (μg / mL) mortality rate(%) Deformity rate (%) Hatching rate (%) 1000 100 0 500 10 0 90 250 0 0 100 125 0 0 100 62.5 0 0 100 31.25 0 0 100 control group 0 0 100

[0222] According to the above experimental data, the non-toxic concentration (NOAEL) of the repair composition is 250 μg / mL.

[0223] 2. Tail Fin Regeneration Model

[0224] Zebrafish: 3 dpf healthy juveniles

[0225] Reagents: TGF-β (10 ng / mL), 0.02% MS-222

[0226] Steps:

[0227] a. Tail fin amputation: 3 dpf healthy juvenile fish were selected and anesthetized with 0.02% MS-222. The posterior 1 / 3 of the tail fin was amputated using microsurgery scissors.

[0228] b. Group treatment, 10 mice per group:

[0229] Example 1 Group: 250 μg / mL repair composition (based on the NOAEL of the initial toxicity screening).

[0230] Positive control group: 10 ng / mL TGF-β.

[0231] Negative control group: E3 medium.

[0232] 3 hpf zebrafish embryos with caudal fin amputated were randomly distributed into well plates, with 10 embryos per well, and 2 mL of Example 1, TGF-β, and E3 culture medium were added respectively.

[0233] c. Regeneration assessment: Pictures were taken at 0, 24, 48, and 72 hours after fin amputation, and the percentage of regenerated area was quantified using ImageJ software (regenerated area / original amputated area × 100%).

[0234] The tail fin regeneration data (72 hours) are shown in Table 9.

[0235] Table 9

[0236]

[0237]

[0238] Based on the above experimental data, it can be seen that the repair composition significantly promotes tail fin regeneration, with an effect close to that of TGF-β. Therefore, it has a good regeneration-promoting effect.

[0239] The above description is merely a preferred embodiment of the present invention and does not constitute any form of limitation to the present invention. Although the present invention has been disclosed as above in terms of a preferred embodiment, it is not intended to limit the present invention. Any person skilled in the art can, without departing from the scope of the technical solution of the present invention, make some changes or modifications to equivalent embodiments using the technical contents disclosed above. However, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A nanoscale repair composition, characterized in that: The invention comprises the following raw materials in parts by weight: 4-6 parts of Centella asiatica extract, 2-4 parts of chamomile extract, 2-4 parts of ginseng extract, 1-3 parts of turmeric extract, 3-5 parts of aloe extract, 1-3 parts of oat extract, 1-3 parts of licorice extract, 0.3-0.7 parts of green tea extract, 0.5-1.5 parts of salvia miltiorrhiza extract, 0.5-1.5 parts of angelica extract, 0.5-1.5 parts of black ganoderma extract, 0.5-1.5 parts of astragalus extract, 0.3-0.7 parts of knotweed extract, and 0.3-0.7 parts of onion extract.

2. The nanoscale repair composition according to claim 1, characterized in that: The nano-scale repair composition comprises the following raw material components by weight: 4-5 parts of Centella asiatica extract, 2-3 parts of chamomile extract, 3-4 parts of ginseng extract, 2-3 parts of turmeric extract, 3-4 parts of aloe extract, 2 parts of oat extract, 2-3 parts of licorice extract, 0.3-0.5 parts of green tea extract, 1 part of salvia miltiorrhiza extract, 1 part of angelica extract, 1 part of black ganoderma extract, 1 part of astragalus extract, 0.5 part of knotweed extract, and 0.5 part of onion extract.

3. A nano-level repair cosmetic, characterized by: The invention comprises the nanoscale repair composition according to any one of claims 1 to 2.

4. The nano-level repair cosmetic according to claim 3, characterized in that: Also includes ceramides, humectants, emollients, thickeners, emulsifiers, preservatives and water.

5. The nano-scale repair cosmetic according to claim 4, characterized in that: The raw material components include the following by weight: 24-30 parts of nano-scale repair composition, 1-3 parts of ceramide, 9-14 parts of moisturizer, 9-15 parts of emollient, 0.3-0.7 parts of thickener, 2-4 parts of emulsifier, 0.8-1.2 parts of preservative, and water added to 100 parts.

6. The nano-level repair cosmetic according to claim 4, characterized in that: The moisturizer includes at least one of glycerin, sodium hyaluronate and panthenol; the emollient includes at least one of squalane, shea butter, jojoba oil, sea buckthorn fruit oil and isononyl isononanoate; the thickener includes xanthan gum, carrageenan and hydroxyethyl cellulose; the emulsifier includes at least one of olive oil emulsifying wax, cetearyl glucoside and polyglyceryl-4 oleate; the preservative includes at least one of phenoxyethanol and ethylhexylglycerin.

7. The nanoscale repair cosmetic according to any one of claims 3 to 7, characterized in that: The composition includes the following raw materials by weight: 5 parts of Centella asiatica extract, 3 parts of chamomile extract, 3 parts of ginseng extract, 2 parts of turmeric extract, 4 parts of aloe extract, 2 parts of oat extract, 2 parts of licorice extract, 0.5 parts of green tea extract, 1 part of salvia miltiorrhiza extract, 1 part of angelica extract, 1 part of black ganoderma extract, 1 part of astragalus extract, 0.5 parts of knotweed extract, 0.5 parts of onion extract, 2 parts of ceramide NP, 8 parts of glycerin, 1.5 parts of sodium hyaluronate, 2 parts of panthenol, 4 parts of squalane, 5 parts of shea butter, 3 parts of jojoba oil, 3 parts of olive oil emulsifying wax, 0.5 parts of xanthan gum, phenoxyethanol, 1 part of ethylhexylglycerin, and 43.5 parts of water.

8. Use of the nanoscale repair cosmetic according to any one of claims 3 to 7 in repairing skin barrier and resisting inflammatory response.

9. A method for preparing the nano-level repair cosmetic according to claim 3, characterized in that: The steps include: (1) Preparation of aqueous phase: Add glycerin and sodium hyaluronate to water, stir until completely dissolved, add the nanoscale repair composition according to any one of claims 1 to 2, add a thickener, and stir until no particles are present; (2) Preparation of oil phase: Heat and stir the emollient and emulsifier until completely melted and mixed, add ceramide, and stir; (3) Emulsification and mixing: slowly pour the oil phase into the water phase and homogenize and emulsify to obtain an emulsion; (4) Cooling and post-treatment: Cool the emulsion, add panthenol and preservatives, mix well, adjust the pH to 5.5-6.0, and filter sterilize.

Images