Eggplant core SNP molecular marker set and application

By developing the core SNP molecular marker set of eggplant and using resequencing and KASP technology, the problems of germplasm resource distinction and variety identification in eggplant breeding are solved, and rapid and accurate variety identification and seed purity detection are achieved, and breeding preparation is optimized.

CN120485410APending Publication Date: 2025-08-15ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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Patent Information

Application Number
CN202510554711.1
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-04-29
Publication Date
2025-08-15

AI Technical Summary

Technical Problem

During the existing eggplant breeding process, it is impossible to effectively distinguish between germplasm resources, variety identification and authenticity detection, and there are phenomena such as "forexual objects of the same name" and "forexual objects of the same name".

Method used

A set of core SNP molecular markers for eggplant was developed, and 23 SNP markers were screened through resequencing technology, and genotyped based on genomic resequencing data, combined with KASP technology to form a DNA fingerprint map for variety identification and seed purity detection.

Benefits of technology

It has achieved rapid and accurate distinction between germplasm resources, simplified the variety identification process, improved authenticity detection efficiency, overcome the phenomena of "foreign objects with the same name" and "foreign objects with the same name", and optimized breeding work preparations.

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Abstract

The invention relates to the technical field of molecular genetic breeding, and particularly discloses an eggplant core SNP molecular marker set and application, and the molecular marker set comprises 23 SNP markers; screening representative eggplant germplasm for re-sequencing; the method comprises the following steps: by taking an eggplant HQ-1315V1.0 genome as a reference genome, carrying out comparison to obtain 4869502 SNP (Single Nucleotide Polymorphism) markers; the method comprises the following steps: screening to obtain a first SNP molecular marker set based on a material discrimination degree, a distribution uniformity degree on a genome, specificity intensity, a heterozygosity degree, a gene diversity index, a polymorphic information amount PIC and a minimum allele frequency MAF principle of SNP markers; 96 SNP molecular markers are randomly selected, and genetic typing verification is carried out on the SNP molecular markers by using eggplant resource materials to obtain 32 candidate SNP molecular markers; and further performing genetic diversity evaluation, clustering analysis and population structure analysis on the 32 candidate SNP molecular markers, and screening to obtain 23 core SNP molecular marker sets. The method has the characteristics that germplasm resources can be effectively distinguished, and variety identification and authenticity detection are convenient.
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