A method for non-targeted screening of hydroxy-esterified glucocorticoids in cosmetics using low-resolution mass spectrometry

Through low-resolution mass spectrometry technology and high-performance liquid chromatography-tandem triple quadrupole mass spectrometry, non-targeted screening of hydroxyester-derived glucocorticoids in cosmetics was achieved, which solved the problems of low efficiency and high cost of existing detection methods, reduced the risk of missed detection, and is suitable for the detection of a variety of new hormones.

CN120490362BActive Publication Date: 2025-09-30大连市检验检测认证技术服务中心
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Patent Information

Application Number
CN202511000641.1
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-07-21
Publication Date
2025-09-30
Estimated Expiration
2045-07-21

AI Technical Summary

Technical Problem

Existing methods for detecting glucocorticoids in cosmetics mainly rely on targeted detection, which has the disadvantages of low detection efficiency, high cost and risk of missed detection. In particular, it is unable to effectively screen new hydroxy-esterified glucocorticoids and cannot meet regulatory requirements.

Method used

Low-resolution mass spectrometry technology is used to add sodium hydroxide and stabilizers to the cosmetic sample solution, and the reaction conditions are controlled to allow the hydroxy-esterified glucocorticoids to be directionally hydrolyzed into non-esterified forms. Non-targeted screening is performed in combination with high-performance liquid chromatography-tandem triple quadrupole mass spectrometry, and the qualitative and quantitative analysis of multiple glucocorticoids is achieved by MRM spectral comparison.

Benefits of technology

It has achieved efficient and low-cost screening of various hydroxy-esterified glucocorticoids, reduced the missed detection rate, expanded the detection range, simplified the operation process, and is suitable for non-targeted detection of various new hormones.

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Abstract

The present invention discloses a method for non-targeted screening of hydroxyl-esterified glucocorticoids in cosmetics using low-resolution mass spectrometry. Specifically, sodium hydroxide at a concentration of 10 mg / mL is added to an acetonitrile solvent system to create an alkaline condition, and a stabilizer consisting of EDTA and sodium ascorbate is added in a fixed amount to the reaction system. The reaction time is controlled to be 1 hour at room temperature. An acetonitrile aqueous solution containing acetic acid is added to adjust the reaction system to an acidic environment and constant volume. Glucocorticoids derived from different esterifications of the hydroxyl groups at positions 21 and / or 17 of the D ring are hydrolyzed and deesterified, thereby returning the product to its unesterified state and stabilizing the product. Furthermore, only one hydroxyl-free glucocorticoid is used as a standard. High-performance liquid chromatography-tandem triple quadrupole mass spectrometry is used to perform non-targeted screening of hydroxyl-esterified glucocorticoids in cosmetics.
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