Microbial screening kit and screening method for pulmonary nodules

Abstract

The invention discloses a microbial screening kit for pulmonary nodules and a screening method, and relates to the technical field of medical detection equipment, the microbial screening kit comprises a shell, the shell is provided with a first screening chamber and a second screening chamber, the first screening chamber is internally provided with a first test tube and pH sensitive gel, and the second screening chamber is internally provided with a second test tube; a plurality of second test tubes and freeze-drying primer probes are arranged in the first screening chamber, a plurality of second test tubes and freeze-drying primer probes are arranged in the second screening chamber, the first screening chamber is further provided with a first temperature control module and an RGB developing detection module, the second screening chamber is provided with a second temperature control module and a fluorescence detection module, and the screening kit further comprises a controller; according to the method, fluorescence abnormity caused by the prevotella and saliva pH value change caused by the actinomycetes are detected, and the two detections assist each other, so that the detection accuracy is improved; meanwhile, rapid detection is realized by using the pH sensitive gel and the freeze-dried primer probe, the screening efficiency is improved, and the kit is particularly suitable for rapid screening on a diagnosis and treatment site.

Description

Technical Field

[0001] The present application relates to the technical field of medical detection equipment, and in particular to a microbial screening kit and screening method for lung nodules. Background Art

[0002] Pulmonary nodules are defined as focal round or quasi-round shadows of increased density ≤3 cm in diameter that appear on lung images. They may be solitary or multiple and may be accompanied by atelectasis and hilar lymphadenopathy. Nowadays, the screening of lung nodules is mainly carried out through medical imaging or lung CT imaging. Although the above diagnostic methods have high diagnostic accuracy, they require going to the hospital to complete the relevant tests, and their detection efficiency is low. At the same time, patients generally only undergo the above screening when symptoms appear, which leads to the delay in the detection of lung nodules, which is not conducive to the early screening of lung nodules and the screening efficiency of lung nodules is low. Summary of the Invention

[0003] The main purpose of this application is to provide a microbial screening kit and screening method for lung nodules, aiming to solve the defect of low screening efficiency in the existing technology.

[0004] This application achieves the above objectives through the following technical solutions: A microbial screening kit for pulmonary nodules, comprising a shell; a first screening chamber, the first screening chamber being disposed on the housing, the first screening chamber being provided with a first test tube, the first test tube being pre-placed with a pH-sensitive gel; a second screening chamber, the second screening chamber being disposed on the housing, wherein a plurality of second test tubes are disposed in the second screening chamber, each of the second test tubes being provided with a freeze-dried primer probe; A first temperature control module, the first temperature control module is used to control the detection temperature of the first screening room, and the first screening room is also provided with an RGB color detection module for color measurement; A second temperature control module, the second temperature control module is used to control the detection temperature of the second screening room; the second screening room is also provided with a fluorescence detection module for measuring the fluorescence value; A controller is electrically connected to the first temperature control module, the RGB color detection module, the second temperature control module and the fluorescence detection module respectively.

[0005] Optionally, the first screening chamber and the second screening chamber are both provided with a detection tube connected thereto, and each detection tube is provided with a sealing gasket or a standard thermometer for temperature calibration.

[0006] Optionally, the shell includes an outer protective shell and an inner thermal insulation shell, and an aerogel thermal insulation layer is provided between the outer protective shell and the inner thermal insulation shell.

[0007] Optionally, the pH sensitive gel contains a bromocresol purple indicator, and the lyophilized primer probe contains a Prevotella-specific primer and a TaqMan probe.

[0008] Optionally, the first temperature control module includes a ceramic heating plate and a thermocouple, the ceramic heating plate is arranged on the side wall of the first screening chamber, and the thermocouple is arranged on the top of the first screening chamber; the ceramic heating plate and the thermocouple are electrically connected to the controller respectively.

[0009] Optionally, the RGB color detection module includes an RGB sensor and a white light source, and the RGB sensor and the white light source are arranged in the first screening room, and the RGB sensor and the white light source are placed on both sides of the first test tube.

[0010] Optionally, the second temperature control module includes a PTC heating plate and an NTC temperature sensor, the PTC heating plate is arranged on the side wall of the second screening chamber, and the NTC temperature sensor is arranged on the top of the second screening chamber, and the PTC heating plate and the NTC temperature sensor are electrically connected to the controller respectively.

[0011] Optionally, the fluorescence detection module includes a fluorescence spectrum sensor and an excitation light source, and the fluorescence spectrum sensor and the excitation light source are arranged in the second screening chamber, and the fluorescence spectrum sensor and the excitation light source are placed on both sides of the second test tube.

[0012] Accordingly, the present application also discloses a screening method based on the above screening kit, comprising the following steps: Setting basic detection parameters, wherein the basic detection parameters include a maximum yellowness reference value, a weight parameter generation formula, and a risk assessment model; Obtain actual fluorescence Ct value and actual colorimetric value; Generating a weight parameter and a risk score calculation formula according to the actual fluorescence Ct value; Calculate the risk score according to the risk score calculation formula; A screening result is output based on the risk score and the risk assessment model.

[0013] Optionally, the expression for the weight parameter generation formula is : The risk score calculation formula is: , where Ct0 represents the actual fluorescence Ct value, Ct max represents the fluorescence saturation threshold, a represents the actual chromaticity value, a max Represents the maximum yellowness reference value, and the expression of the risk assessment model is: .

[0014] Compared with the prior art, this application has the following beneficial effects: The present application includes a shell, on which a first screening chamber and a second screening chamber are provided. A first test tube is provided in the first screening chamber, in which a pH-sensitive gel is pre-placed. A plurality of second test tubes are provided in the second screening chamber, in which a freeze-dried primer probe is provided. At the same time, a first temperature control module and an RGB color development detection module for color measurement are also provided in the first screening chamber, and a second temperature control module and a fluorescence detection module for measuring fluorescence values are provided in the second screening chamber. The screening kit also includes a controller, which is electrically connected to the first temperature control module, the RGB color development detection module, the second temperature control module and the fluorescence detection module respectively; Correspondingly, the present application also discloses a corresponding screening method, which first sets basic detection parameters, then obtains the actual fluorescence Ct value and the actual colorimetric value, then generates a weight parameter and a risk score calculation formula based on the actual fluorescence Ct value, then calculates the risk score according to the risk score calculation formula, and finally outputs the screening result based on the risk score and the risk assessment model; During the screening process, the temperature of the first screening room is adjusted to the optimal detection temperature through the first temperature control module, and the temperature of the second screening room is adjusted to the optimal detection temperature through the second temperature control module, while ensuring that the temperature fluctuations in the two screening rooms always meet the requirements; The lesions in patients with pulmonary nodules will lead to abnormal metabolism of Actinomycetes and Prevotella in the human oral cavity. Abnormal proliferation of Actinomycetes will cause the human saliva to become acidic, causing the pH-sensitive gel to turn from purple to yellow. The abundance of Prevotella will also increase significantly, leading to abnormal fluorescence detection. Targeted measurement of these characteristics can achieve early screening of patients with pulmonary nodules. Compared with the existing technology, the present application not only detects fluorescence abnormalities caused by Prevotella, but also detects changes in saliva pH caused by actinomycetes. The two tests complement and confirm each other, which is conducive to improving the accuracy of detection. For example, the fluorescence abnormality detection can exclude false positives caused by changes in saliva pH caused by the activity of other bacterial flora; Secondly, the screening kit described in this application has a simple structure. While reducing equipment costs, it enables rapid implementation of relevant detection through the use of pH-sensitive gel and freeze-dried primer probes, thereby improving the screening efficiency of lung nodules and is particularly suitable for rapid screening at the diagnosis and treatment site. In terms of screening methods, this application cannot use a weight calculation formula to calculate the risk score, thereby taking into account the actual fluorescence Ct value and the actual colorimetric value, ensuring the accuracy of the calculation. At the same time, a weight parameter calculation link is added. Through the calculation of the weight parameter, not only can the proportion of the actual fluorescence Ct value and the actual colorimetric value be flexibly adjusted, but the algorithm can also be flexibly adjusted when the actual fluorescence Ct value is abnormal, thereby reducing the proportion of the actual fluorescence Ct value, avoiding calculation errors caused by parameter abnormalities, and improving the accuracy of the evaluation results. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 A schematic structural diagram of a microbial screening kit for pulmonary nodules provided in embodiment 1 of the present application; Figure 2 An exploded view of a microbial screening kit for pulmonary nodules provided in embodiment 1 of the present application; Figure 3 A cross-sectional view of a microbial screening kit for pulmonary nodules provided in embodiment 1 of the present application; Figure 4 Flow chart of the screening method provided in embodiment 2 of this application; Figure markings: 1-shell, 2-first screening chamber, 3-first test tube, 4-second screening chamber, 5-second test tube, 6-controller, 7-detection tube, 8-sealing pad, 9-standard thermometer, 10-ceramic heating plate, 11-thermocouple, 12-RGB sensor, 13-white light source, 14-PTC heating plate, 15-NTC temperature sensor, 16-fluorescence spectrum sensor, 17-excitation light source, 101-outer protective shell, 102-inner insulation shell, 103-aerogel insulation layer.

[0016] The purpose, features and advantages of this application will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION

[0017] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.

[0018] It should be noted that all directional indications (such as up, down, left, right, front, back, etc.) in the embodiments of the present invention are only used to explain the relative position relationship, movement status, etc. between the various components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication will also change accordingly.

[0019] In the present invention, unless otherwise specified or limited, the terms "connection" and "fixation" should be understood in a broad sense. For example, "fixation" can mean fixed connection, detachable connection, or integration; mechanical connection or electrical connection; direct connection or indirect connection through an intermediate medium; internal communication between two elements or interaction between two elements, unless otherwise specified. Those skilled in the art will be able to understand the specific meanings of the above terms in the present invention based on specific circumstances.

[0020] In addition, if there are descriptions involving "first", "second", etc. in the embodiments of the present invention, the descriptions of "first", "second", etc. are only for descriptive purposes and cannot be understood as indicating or suggesting their relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of such features. In addition, the meaning of "and / or" appearing throughout the text includes three parallel schemes. Taking "A and / or B" as an example, it includes scheme A, or scheme B, or a scheme in which A and B are satisfied at the same time. In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the ability of ordinary technicians in this field to implement. When the combination of technical solutions is mutually contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0021] Implementation Method 1 Reference Figures 1 to 3 This embodiment, as an optional embodiment of the present application, discloses a microbial screening kit for pulmonary nodules, comprising a housing 1, wherein the housing 1 comprises an outer protective shell 101 and an inner thermal insulation shell 102, wherein the outer protective shell 101 is made of engineering plastic, and the inner thermal insulation shell 102 is made of stainless steel; an aerogel insulation layer 103 made of aerogel is filled between the outer protective shell 101 and the inner thermal insulation shell 102; Along the height direction of the housing 1, a first screening chamber 2 is provided on the upper side of the housing 1, and a second screening chamber 4 is provided on the lower side thereof; The above structural design not only improves the wear resistance of the equipment, but also effectively improves the heat preservation performance of the entire screening kit, thereby ensuring the temperature stability in the first screening chamber 2 and the second screening chamber 4, avoiding detection errors caused by drastic temperature fluctuations, and improving detection accuracy; A detection plate is slidably installed in both the first screening chamber 2 and the second screening chamber 4. The detection plate has an L-shaped structure as a whole. A plurality of sliders are provided on the bottom surface of the horizontal section of the detection plate. Two mutually parallel slide rails are provided on the bottom surface of the first screening box. Each slider is slidably connected to the two slide rails. A plurality of sealing strips are also provided on the vertical section of the detection plate. When the detection plate is fully inserted into the first screening chamber 2, the vertical section of the detection plate completely closes the open end of the first screening chamber 2, and the sealing strip is tightly fitted with the side surface of the open end of the first screening chamber 2. A locking block is also rotatably provided on the shell 1. It should be noted that the detection plate is an opaque plate to avoid interference with the detection by ambient light; The second screening chamber 4 has a detection board of the same structure; In the above structure, the user can easily pull out the detection board from the corresponding screening room, and at the same time, the corresponding screening room can be shielded and sealed when the detection board is inserted, thereby ensuring the detection environment load requirements. The device has a simple structure and is easy to operate. Furthermore, a first test tube 3 is provided on the detection plate of the first screening chamber 2, and a second test tube 5 is provided on the detection plate of the second screening chamber 4. The first test tube 3 is pre-placed with a pH-sensitive gel; and the second test tube 5 is pre-placed with a freeze-dried primer probe. Wherein, the pH sensitive gel contains bromocresol purple indicator, and the lyophilized primer probe contains Prevotella-specific primer and TaqMan probe; pH-sensitive gel and freeze-dried primer probes can be used to quickly detect Actinomycetes and Prevotella, respectively, which is beneficial to improve the timeliness of detection; Furthermore, a first temperature control module and an RGB color detection module are provided in the first screening chamber 2, wherein the first temperature control module includes a ceramic heating plate 10 and a thermocouple 11, and the RGB color detection module includes an RGB sensor 12 and a white light source 13; With the insertion direction of the test board in the first screening room 2 as the front, mark the side walls of the screening room as the left side wall, the right side wall, the top side wall and the front side wall respectively; The thermocouple 11 is disposed on the top side wall of the first screening chamber 2, the ceramic heating plate 10 is disposed on the front side wall, and the RGB sensor 12 and the white light source 13 are disposed on the left side wall or the right side wall, that is, the RGB sensor 12 and the white light source 13 are disposed on both sides of the first test tube 3; The above arrangement ensures that the thermocouple 11 is in the core detection area, thereby ensuring the accuracy of temperature detection in the core detection area; on the other hand, it can separate the first temperature control module and the RGB color detection module to avoid mutual interference between the two, which is conducive to improving the accuracy of color recognition and thus improving the accuracy of early screening. It should be noted that using a white light source 13 as the light source can reduce the interference of the light source color on the detection.

[0022] The second screening chamber 4 is provided with a second temperature control module and a fluorescence detection module, wherein the second temperature control module includes a PTC heating plate 14 and an NTC temperature sensor 15, and the fluorescence detection module includes a fluorescence spectrum sensor 16 and an excitation light source 17; Similarly, with the insertion direction of the test board in the second screening chamber 4 as the front, the side walls of the screening chamber are marked as the left side wall, the right side wall, the top side wall and the front side wall respectively; The NTC temperature sensor 15 is disposed on the top side wall, the PTC heating plate is disposed on the front side wall, and the fluorescence spectrum sensor 16 and the excitation light source 17 are disposed on the left and right side walls, respectively. That is, the fluorescence spectrum sensor 16 and the excitation light source 17 are disposed on both sides of the second test tube 5; It should be noted that the excitation light source 17 is preferably an LED lamp other than 365nm, and the excitation light source 17 needs to be integrated with a dichroic mirror and a bandpass filter to separate the excitation light and the fluorescence to improve the accuracy of the detection; In the above technical solution, the first temperature control module includes a ceramic heater 10 and a thermocouple 11, and the second temperature control module uses a PTC heater 14 and an NTC temperature sensor 15. The color screening has a lower requirement for the screening temperature. Therefore, the above equipment can reduce the cost of the equipment while meeting the use requirements, and the second temperature control module can achieve higher-precision temperature regulation. Furthermore, two detection tubes 7 are provided on the housing 1. The two detection tubes 7 are independent of each other, one of which is connected to the first screening chamber 2, and the other is connected to the second screening chamber 4. Under normal working conditions, the two detection tubes 7 are sealed with a sealing gasket 8. When temperature calibration is required, a standard thermometer 9 is inserted into the detection tube 7. By using the standard thermometer 9, the staff can calibrate each temperature sensor at any time to ensure its measurement accuracy and improve the accuracy of screening. At the same time, the calibration operation is simple and convenient, which can improve the calibration efficiency.

[0023] Furthermore, a controller 6 is provided on the housing 1, and the controller 6 includes a control board and a touch screen that are communicatively connected, wherein the control board is integrated with a microprocessor, preferably, the microprocessor model can be STM32F407, and the controller 6 also includes a lithium battery for power supply; The control board is electrically connected to the first temperature control module, the RGB color development detection module, the second temperature control module and the fluorescence detection module respectively.

[0024] Implementation Method 2 Reference Figure 4 This embodiment, as another optional embodiment of the present application, discloses a method for screening lung nodules, comprising the following steps: S1. Setting basic detection parameters, wherein the basic detection parameters include a maximum yellowness reference value, a weight parameter generation formula, and a risk assessment model; Setting basic detection parameters according to actual conditions or directly using preset values as basic detection parameters; wherein the basic detection parameters include a maximum yellowness reference value, a weight parameter generation formula, and a risk assessment model; The expression of the weight parameter generation formula is: , the expression of the risk assessment model is: , where Ct0 represents the actual fluorescence Ct value, Ct max represents the fluorescence saturation threshold; It should be noted that the parameters in the weight parameter generation formula can be adjusted. The above adjustment gives the operator different weight preferences, thereby improving the accuracy of the detection; It should also be noted that Ct max It represents the fluorescence saturation threshold, which is actually an inherent parameter of the device. When the actual fluorescence Ct value exceeds this number, due to the limitations of the detection method, the distortion of the measurement value of the fluorescence spectrum sensor will increase sharply, that is, it will gradually lose its reference significance. By setting the weight parameter generation formula, this application can reduce the weight of the fluorescence value in the screening through automatic adjustment of the weight parameter in this case, and increase the weight of the more stable and reliable chromaticity value, thereby ensuring the accuracy and reliability of the screening results.

[0025] The maximum yellowness value is obtained by extracting saliva from multiple patients diagnosed with pulmonary nodules and performing a color development test, and then combining it with a standard color card; S2, obtaining the actual fluorescence Ct value and the actual colorimetric value; Start the equipment and pre-treat the collected saliva to be tested. The treated saliva is divided into two portions as needed, one of which is dripped into the first test tube and the second into the second test tube. The first and second test tubes are then placed on corresponding test plates and the first and second screening chambers are closed. It should be noted that before the test, the first temperature control module and the second temperature control module need to adjust the temperature in the first screening room and the second screening room to the optimal detection temperature to avoid the adverse effects of the temperature rise process on the test; Finally, the actual fluorescence Ct value and actual colorimetric value are obtained; S3. Generating a weight parameter and a risk score calculation formula according to the actual fluorescence Ct value; First, the expression for the actual fluorescence Ct value and weight parameter generation formula is obtained as follows: , comparing the actual fluorescence Ct value and the fluorescence saturation threshold to generate a corresponding weight parameter; Then, the corresponding risk score calculation formula is generated according to the weight parameters; the expression of the risk score calculation formula is: , where Ct0 represents the actual fluorescence Ct value, Ct max represents the fluorescence saturation threshold, a represents the actual chromaticity value, a max Indicates the maximum yellowness reference value; In the above-mentioned risk score calculation formula, not only are corresponding weight parameters set, but also, according to medical practice, when Ct ≤ 35, it can be determined to be positive for Prevotella, and the lower the value, the higher the Prevotella load, that is, 35 is the cutoff threshold for Prevotella; in the risk score calculation formula of this application, the smaller the actual fluorescence Ct value, the closer the value of Prevotella is to its weight value, such as approaching 0.6, thereby making the risk score positively correlated with the load of Prevotella; the above-mentioned technical principles are also applicable to colorimetric values, thereby ensuring the accuracy of the calculation results; Secondly, in the above calculation, the ratio of the two terms also realizes the normalization of the numbers, which is conducive to improving the accuracy of parameter calculation.

[0026] Substituting the measured actual fluorescence Ct value and actual colorimetric value into the risk score calculation formula can obtain the risk score; S4. Calculate the risk score according to the risk score calculation formula; Retrieve the risk score calculation formula. The risk score calculation formula is as follows: , where Ct0 represents the actual fluorescence Ct value, Ct max represents the fluorescence saturation threshold, a represents the actual chromaticity value, a max Indicates the maximum yellowness reference value; The risk score can be calculated by simultaneously obtaining the actual fluorescence Ct value and the actual colorimetric value measured in step S2.

[0027] S5. Output a screening result according to the risk score and the risk assessment model.

[0028] The expression for calling the risk assessment model is: and the risk score calculated in step S4, and outputting the corresponding result from the risk assessment model according to the score as the screening result output.

[0029] It should be noted that the high risk in the above risk assessment model means that the probability of the subject being tested having lung nodules is high, and the subject being tested needs to be paid special attention or further examined for a confirmed diagnosis. The test result cannot be used as the final health judgment result. The above judgment result is the intermediate result of the diagnosis of lung nodules. Medium risk and low risk cannot be used as the final health judgment result.

[0030] Compared with the existing technology, the present application not only detects fluorescence abnormalities caused by Prevotella, but also detects changes in saliva pH caused by actinomycetes. The two tests complement and confirm each other, which is conducive to improving the accuracy of detection. For example, the fluorescence abnormality detection can exclude false positives caused by changes in saliva pH caused by the activity of other bacterial flora; Secondly, the screening kit described in this application has a simple structure. While reducing equipment costs, it enables rapid implementation of relevant detection through the use of pH-sensitive gel and freeze-dried primer probes, thereby improving the screening efficiency of lung nodules and is particularly suitable for rapid screening at the diagnosis and treatment site. In terms of screening methods, this application cannot use a weight calculation formula to calculate the risk score, thereby taking into account the actual fluorescence Ct value and the actual colorimetric value, ensuring the accuracy of the calculation. At the same time, a weight parameter calculation link is added. Through the calculation of the weight parameter, not only can the proportion of the actual fluorescence Ct value and the actual colorimetric value be flexibly adjusted, but the algorithm can also be flexibly adjusted when the actual fluorescence Ct value is abnormal, thereby reducing the proportion of the actual fluorescence Ct value, avoiding calculation errors caused by parameter abnormalities, and improving the accuracy of the evaluation results.

[0031] The above are only preferred embodiments of the present application and do not limit the patent scope of the present application. Any equivalent structure or equivalent process transformation made using the contents of the present application specification and drawings, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present application.

Claims

1. A microbial screening kit for pulmonary nodules, characterized in that: comprising a housing (1); A first screening chamber (2), the first screening chamber (2) being arranged on the housing (1), a first test tube (3) being arranged in the first screening chamber (2), and a pH-sensitive gel being pre-placed in the first test tube (3); A second screening chamber (4), the second screening chamber (4) being arranged on the housing (1), a plurality of second test tubes (5) being arranged in the second screening chamber (4), and a freeze-dried primer probe being arranged in each of the second test tubes (5); a first temperature control module, the first temperature control module being used to control the detection temperature of the first screening chamber (2); an RGB color development detection module for color measurement being further provided in the first screening chamber (2); a second temperature control module, the second temperature control module being used to control the detection temperature of the second screening chamber (4); a fluorescence detection module for measuring fluorescence values is also provided in the second screening chamber (4); A controller (6) is electrically connected to the first temperature control module, the RGB color development detection module, the second temperature control module, and the fluorescence detection module.

2. A microbial screening kit for pulmonary nodules according to claim 1, characterized in that: The first screening chamber (2) and the second screening chamber (4) are both provided with a detection tube (7) connected thereto, and each detection tube (7) is provided with a sealing gasket (8) or a standard thermometer (9) for temperature calibration.

3. A microbial screening kit for pulmonary nodules according to claim 1, characterized in that: The shell (1) comprises an outer protective shell (101) and an inner thermal insulation shell (102), and an aerogel thermal insulation layer (103) is provided between the outer protective shell (101) and the inner thermal insulation shell (102).

4. A microbial screening kit for pulmonary nodules according to claim 1, characterized in that: The pH sensitive gel contains a bromocresol purple indicator, and the lyophilized primer probe contains a Prevotella-specific primer and a TaqMan probe.

5. A microbial screening kit for pulmonary nodules according to claim 1, characterized in that: The first temperature control module comprises a ceramic heating plate (10) and a thermocouple (11); the ceramic heating plate (10) is arranged on the side wall of the first screening chamber (2), and the thermocouple (11) is arranged on the top of the first screening chamber (2); the ceramic heating plate (10) and the thermocouple (11) are respectively electrically connected to the controller (6).

6. A microbial screening kit for pulmonary nodules according to claim 5, characterized in that: The RGB color development detection module comprises an RGB sensor (12) and a white light source (13), wherein the RGB sensor (12) and the white light source (13) are arranged in the first screening chamber (2), and the RGB sensor (12) and the white light source (13) are respectively placed on both sides of the first test tube (3).

7. A microbial screening kit for pulmonary nodules according to claim 1, characterized in that: The second temperature control module comprises a PTC heating plate (14) and an NTC temperature sensor (15); the PTC heating plate (14) is arranged on the side wall of the second screening chamber (4); the NTC temperature sensor (15) is arranged on the top of the second screening chamber (4); the PTC heating plate (14) and the NTC temperature sensor (15) are respectively electrically connected to the controller (6).

8. A microbial screening kit for pulmonary nodules according to claim 7, characterized in that: The fluorescence detection module comprises a fluorescence spectrum sensor (16) and an excitation light source (17), wherein the fluorescence spectrum sensor (16) and the excitation light source (17) are arranged in the second screening chamber (4), and the fluorescence spectrum sensor (16) and the excitation light source (17) are respectively placed on both sides of the second test tube (5).

9. A screening method based on a microbial screening kit for pulmonary nodules according to any one of claims 1 to 8, characterized in that: The following steps are involved: Setting basic detection parameters, wherein the basic detection parameters include a maximum yellowness reference value, a weight parameter generation formula, and a risk assessment model; Obtain actual fluorescence Ct value and actual colorimetric value; Generating a weight parameter and a risk score calculation formula according to the actual fluorescence Ct value; Calculate the risk score according to the risk score calculation formula; A screening result is output based on the risk score and the risk assessment model.

10. A screening method according to claim 9, characterized in that: The expression of the weight parameter generation formula is: , the risk score calculation formula is: , where Ct0 represents the actual fluorescence Ct value, Ct max represents the fluorescence saturation threshold, a represents the actual chromaticity value, a max Represents the maximum yellowness reference value, and the expression of the risk assessment model is: .

Images