Rapid enzyme activity detection and addition method in enzymatic pretreatment of cotton fabric

Abstract

The invention discloses a method for rapidly detecting and adding enzyme activity in enzymatic pretreatment of cotton fabrics, and belongs to the field of ecological dyeing and finishing of textiles. The method for rapidly detecting and adding the enzyme activity in the enzyme pretreatment of the cotton fabric is carried out based on a system. The system comprises a user interface, a main control board, an enzyme liquid tank, a substrate solution pool, a reaction pool, a developing solution pool, a high-concentration enzyme liquid box and a spectrophotometer. Wherein the enzyme liquid tank, the substrate solution and the color developing solution are obtained through the liquid taking pump for reaction color development in the reaction tank and are detected through the spectrophotometer, the detection result is transmitted to the main control board, the enzyme activity and the addition amount of the high-concentration enzyme liquid are calculated through a built-in formula of the main control board, and the enzyme adding pump is started through the main control board; quantitative enzyme liquid is sucked from the high-concentration enzyme liquid box to the enzyme liquid tank, and one-time enzyme activity rapid detection and adding process is completed; compared with a traditional mode, the method has the advantages that enzyme supplement is efficient, and the pretreatment process and product quality are stable.

Description

Technical Field

[0001] The invention relates to a method for rapid detection and additional addition of enzyme activity in enzymatic pretreatment of cotton fabrics, and belongs to the field of ecological dyeing and finishing of textiles. Background Art

[0002] The core advantages of bio-enzymatic pretreatment for cotton fabrics are environmental friendliness and high energy efficiency. Compared to traditional high-alkaline, high-temperature processes, enzymatic treatment operates under mild conditions, reducing energy consumption by 30%-50%, water consumption by over 20%, and COD levels in wastewater by up to 40%. Amylase desizing precisely breaks down the sizing without damaging the fibers. Pectinase, in tandem with cellulase, selectively removes pectin impurities, preserving natural waxes and imparting a soft feel to the fabric. Bio-pretreatment effectively avoids the hydrolysis and oxidative damage caused by strong alkali, increasing fabric strength retention by 15%-20% and significantly improving whiteness and hair efficiency. The enzyme preparation is biodegradable, produces no toxic byproducts, and reduces wastewater treatment load by 60%, aligning with the development of green textiles. This technology simultaneously improves quality and efficiency while maintaining clean production, reducing overall processing costs by 8%-12%, making it a highly sought-after ecological dyeing and finishing process in the cotton printing and dyeing industry.

[0003] However, during continuous enzymatic pretreatment of cotton fabrics (primarily desizing and scouring), enzyme activity decreases to varying degrees due to the elevated temperatures in the mangle tank as the exchange time between the enzyme solution and the fabric increases. This reduced enzyme activity can, to varying degrees, impact the stability of subsequent pretreatment results.

[0004] To address these issues, the current continuous enzymatic pretreatment process for cotton fabrics often involves intermittently measuring enzyme solution from the enzyme tank where the cotton fabric is padded. Enzyme activity is then tested in the laboratory, and a prescribed amount of high-concentration enzyme solution is added to the padded tank based on the results. This method suffers from a long enzyme activity measurement cycle, making it difficult to efficiently and timely replenish the padded tank with enzyme activity, which can easily lead to quality fluctuations in the cotton fabric semi-finished product. Therefore, how to dynamically monitor and replenish enzyme activity in the padded tank during the cotton fabric pretreatment process, as required by continuous pretreatment production, is a pressing challenge that needs to be addressed and is crucial for improving the quality of cotton fabric semi-finished products. Summary of the Invention

[0005] [Technical Issues]

[0006] In the continuous enzymatic pretreatment of cotton fabrics, the activity of amylase and pectinase tends to decrease at high temperatures, resulting in a decrease in enzyme activity in the enzyme liquid tank when the cotton fabric is dipped and rolled, affecting the processing quality of the cotton fabric semi-finished product. The manual determination and addition of enzyme activity are inefficient. The above-mentioned shortcomings affect the stability of the enzymatic pretreatment effect of cotton fabrics.

[0007] [Technical solution]

[0008] To solve the above problems, the purpose of the present invention is to provide a method for rapid detection and addition of enzyme activity in the enzymatic pretreatment of cotton fabrics. The method detects the enzyme activity in the manifold by combining interval sampling from the manifold with spectral quantitative analysis, and timely adds high-concentration enzyme solution based on the decrease in enzyme activity, thereby effectively solving the problem of instability in the processing effect of the enzymatic pretreatment of cotton fabrics.

[0009] In order to achieve the above objectives, the technical solutions provided are as follows:

[0010] The first object of the present invention is to provide a system for rapid detection and addition of enzyme activity in enzymatic pretreatment of cotton fabrics, the system comprising a user interface, a main control panel, an enzyme solution tank, a substrate solution tank, a reaction tank with a heating function, a color development solution tank, a high-concentration enzyme solution tank, and a spectrophotometer;

[0011] Among them, the reaction pool is used for the reaction and color development of enzyme solution and substrate solution; the enzyme solution tank is used to store enzyme solution for cotton fabric padding and is connected to the reaction pool through a liquid pump 1; the substrate solution tank is used to store substrate solution for enzyme reaction and is connected to the reaction pool through a liquid pump 2; the color development liquid tank is used to store solution for color development after the enzyme and substrate react and is connected to the reaction pool through a liquid pump 3; the high-concentration enzyme liquid tank is used to store high-concentration enzyme solution for additional use and is connected to the enzyme solution tank through an enzyme addition pump; the spectrophotometer is used to measure the absorbance after the reaction pool develops color and transmits the collected absorbance to the main control board;

[0012] The main control board is used to coordinate various parts of the system, collect system parameters, and use built-in formulas to calculate enzyme activity and the amount of high-concentration enzyme solution to be added; the user interface is used to display the current measured enzyme activity and set the target enzyme activity and total volume of the enzyme solution in the enzyme tank.

[0013] In one embodiment, the system further comprises a robotic arm for completing liquid collection, pipetting and placement in the system, which is controlled by a main control board.

[0014] In one embodiment, the enzyme adding pump is controlled by the main control board to add the amount of high-concentration enzyme and injects it into the enzyme liquid tank.

[0015] In one embodiment, the built-in formula includes:

[0016] Standard curve A corresponding to absorbance and aldose concentration i =k×C i , where k is the molar absorptivity;

[0017] Calculation of enzyme concentration difference: Based on absorbance A i 'Value, the concentration of the corresponding hydrolyzate C is obtained by comparing the standard curvei 'mg / mL, on this basis, the enzyme activity a' value in the enzyme tank is calculated as:

[0018] Enzyme activity a'=C'×V2 / (V1×t), where C'=A i ' / k, V2 is the total volume of the mixed solution in the reaction tank, V1 is the volume of the enzyme solution drawn from the enzyme tank, and t is the reaction time;

[0019] Calculation of high-concentration enzyme solution replenishment: Based on the difference between the calculated enzyme activity a' and the initial enzyme activity a in the initial enzyme solution tank, calculate the required high-concentration enzyme solution addition amount V3:

[0020] The amount of high-concentration enzyme solution added V3 = (a-a') × V0 / a0, where a0 is the enzyme activity concentration in the high-concentration enzyme solution tank and V0 is the total volume of the enzyme solution in the squeeze tank.

[0021] A second object of the present invention is to provide a method for rapid detection and addition of pretreatment enzyme activity in cotton fabrics based on the above system, the method comprising the following steps:

[0022] (1) Drawing of the standard curve of enzymatic hydrolysis products

[0023] The standard curve A was prepared using 3,5-dinitrosalicylic acid and aldose. i =k×C i , where k is the molar absorption coefficient, built into the main control board;

[0024] (2) Color development and determination of reaction solution

[0025] When starting the test, the target enzyme activity a U / mL of the enzyme tank and the total volume V0 of the enzyme solution in the enzyme tank are input into the user interface. The liquid pump 1 is started to draw a volume V1 of enzyme solution from the enzyme tank where the cotton fabric is padded, and is injected into the reaction tank with the help of a robotic arm. The liquid pump 2 is started to draw a substrate solution from the substrate solution tank and introduce it into the reaction tank with the help of a robotic arm. After keeping the solution at a temperature of 50-60°C for 5-10 minutes (the reaction time is defined as t), the liquid pump 3 is started to draw the DNS solution from the color development liquid tank into the reaction tank to obtain a mixed solution with a total volume of V2. The solution is boiled to produce a color reaction, and the solution is drawn from the reaction tank into the sample cell of the spectrophotometer with the help of a robotic arm, and the absorbance A is measured at 540nm. i ';

[0026] Absorbance A i 'Transmit to the main control board for calculation;

[0027] Calculation of enzyme concentration difference: Based on absorbance A i 'Value, the concentration of the corresponding hydrolyzate C is obtained by comparing the standard curve i'mg / mL, on this basis, the enzyme activity a' value in the enzyme tank is calculated as:

[0028] Enzyme activity a'=C'×V2 / (V1×t), where C'=A i ' / k, V2 is the total volume of the mixed solution in the reaction tank, V1 is the volume of the enzyme solution drawn from the enzyme tank, and t is the reaction time;

[0029] Calculation of high-concentration enzyme solution replenishment: Based on the difference between the calculated enzyme activity a' and the initial enzyme activity a in the initial enzyme solution tank, calculate the required high-concentration enzyme solution addition amount V3:

[0030] The amount of high-concentration enzyme solution added V3 = (a-a') × V0 / a0, where a0 is the enzyme activity concentration in the high-concentration enzyme solution tank and V0 is the total volume of the enzyme solution in the manifold tank;

[0031] (3) Start the enzyme adding pump through the main control panel to draw a fixed amount of enzyme solution from the high-concentration enzyme solution tank into the enzyme solution tank, completing a rapid enzyme activity detection and addition process.

[0032] In one embodiment, in the standard curve drawing of step (1), when detecting the enzymatic activity of amylase, the aldose is glucose; when detecting the enzymatic activity of pectinase, the aldose is D-galacturonic acid.

[0033] In one embodiment, the standard curve construction process in step (1) is specifically as follows:

[0034] Dissolve 3,5-dinitrosalicylic acid, potassium sodium tartrate, caustic soda, phenol and sodium sulfite in water to obtain DNS solution; prepare a series of concentrations C i aldose solution, and add DNS solution respectively, shake well and boil to develop color, cool to room temperature and make up to volume, measure absorbance A at 540nm i ; Standard curve A corresponding to absorbance and aldose concentration i =k×C i ; where k is the molar absorptivity.

[0035] In one embodiment, the specific preparation process of the DNS solution in step (1) is as follows: 10 g of 3,5-dinitrosalicylic acid, 200 g of potassium sodium tartrate, 10 g of caustic soda, 3 g of phenol, and 5 g of sodium sulfite are mixed and dissolved in water and the volume is adjusted to 1000 mL.

[0036] In one embodiment, the series of concentrations C in step (1) i The aldose solutions were 0, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, and 1.0 mg / mL aldose solutions.

[0037] In one embodiment, the mixing volume ratio of the aldose solution and the DNS solution in step (1) is 1:1, and the mixed solution is diluted 5 times for detection.

[0038] In one embodiment, the boiling time in step (1) is 8 to 10 minutes.

[0039] In one embodiment, the boiling time in step (2) is 8 to 10 minutes.

[0040] In one embodiment, the substrate concentration in step (2) is 10 to 30 g / L.

[0041] In one embodiment, the volume ratio of the enzyme solution, substrate solution and DNS solution in the reaction pool of step (2) is 2-5:1:1.

[0042] In one embodiment, in step (2), during the color development and determination of the reaction solution, when detecting the enzymatic activity of amylase, the substrate solution is a soluble starch solution; when detecting the enzymatic activity of pectinase, the substrate solution is a pectin solution.

[0043] The third object of the present invention is to provide an application of the above-mentioned system in textile processing.

[0044] Beneficial effects of the present invention:

[0045] The present invention uses a system-integrated approach to rapidly detect and add enzyme activity during enzymatic pretreatment of cotton fabrics. Compared with traditional enzyme activity detection and addition methods based on manual sampling, calculation, and addition, it has the following advantages:

[0046] (1) Efficient enzyme supplementation in cotton pretreatment: The method of the present invention is used to detect the enzyme concentration and timely add enzymes during the enzymatic pretreatment of cotton fabrics. Operations such as sampling of amylase or pectinase solutions in enzyme tanks, enzyme activity determination, and high-concentration enzyme addition can be completed efficiently in a short time, thereby achieving precise control of the enzymatic pretreatment process of cotton fabrics.

[0047] (2) Stable pretreatment process and product quality: The method of the present invention avoids the disadvantages of cotton fabric pretreatment quality fluctuation caused by the continuous decline of amylase or pectinase activity in the enzyme liquid tank during the enzymatic pretreatment of cotton fabric, which is beneficial to improving the stability of the quality of cotton fabric semi-finished products.

[0048] (3) Intelligent printing and dyeing production and processing: The system described in the present invention realizes the automated control of the enzymatic pretreatment process, which has a significant promoting effect on improving the intelligence and efficiency of the cotton fabric printing and dyeing production process. BRIEF DESCRIPTION OF THE DRAWINGS

[0049] Figure 1The present invention is a schematic flow chart of a system for rapid detection and addition of enzyme activity in enzymatic pretreatment of cotton fabrics. DETAILED DESCRIPTION

[0050] The technical solutions in the embodiments of the present invention will be described clearly and completely below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention. The following specific embodiments further describe the present invention.

[0051] The system for rapid detection and addition of enzyme activity in enzymatic pretreatment of cotton fabrics adopted in the embodiment of the present invention includes a user interface, a main control board, a robotic arm, an enzyme liquid tank, a substrate solution tank, a reaction tank with a heating function, a color development liquid tank, a high-concentration enzyme liquid tank, liquid extraction pumps 1, 2, 3, a spectrophotometer, and an enzyme addition pump.

[0052] Among them, the reaction pool is used for the reaction and color development of enzyme solution and substrate solution; the enzyme solution tank is used to store enzyme solution for cotton fabric padding and is connected to the reaction pool through a liquid pump 1; the substrate solution tank is used to store substrate solution for enzyme reaction and is connected to the reaction pool through a liquid pump 2; the color development liquid tank is used to store solution for color development after the enzyme and substrate react and is connected to the reaction pool through a liquid pump 3; the high-concentration enzyme liquid tank is used to store high-concentration enzyme solution for additional use and is connected to the enzyme solution tank through an enzyme addition pump; the spectrophotometer is used to measure the absorbance after the reaction pool develops color and transmits the collected absorbance to the main control board;

[0053] The main control board is used to coordinate various parts of the system, collect system parameters, and use built-in formulas to calculate enzyme activity and the amount of high-concentration enzyme solution to be added; the user interface is used to display the current measured enzyme activity and set the target enzyme activity and total volume of the enzyme solution in the enzyme tank.

[0054] Example 1

[0055] A method for rapid detection and addition of enzyme activity in enzymatic pretreatment of cotton fabrics based on the above system comprises the following steps:

[0056] (1) Drawing of the standard curve of enzymatic hydrolysis products

[0057] The standard curve was prepared by using 3,5-dinitrosalicylic acid and glucose. Specifically, 10 g of 3,5-dinitrosalicylic acid, 200 g of potassium sodium tartrate, 10 g of caustic soda, 3 g of phenol, and 5 g of sodium sulfite were mixed and dissolved in water to a volume of 1000 mL to obtain DNS solution. 10 mL of concentration C i10 mL of DNS solution was added to glucose solutions of 0, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, and 1.0 mg / mL, respectively. The mixture was shaken and boiled for 8 minutes to develop color. After cooling to room temperature, the solution was diluted to 100 mL and the absorbance A was measured at 540 nm. i , and obtain the standard curve A corresponding to absorbance and glucose concentration i =k×C i (where k is the molar absorptivity);

[0058] (2) Color development and determination of reaction solution

[0059] When starting the test, enter the target amylase activity of 5.2U / mL and the total volume of 30L of amylase solution in the enzyme tank in the user interface, start the liquid pump 1, draw 2.5mL of enzyme solution with a volume of V1 from the enzyme tank where the cotton fabric is padded, and inject it into the reaction tank with the help of a robotic arm; start the liquid pump 2, draw 50mL of soluble starch solution with a concentration of 10g / L from the substrate solution tank, and introduce it into the reaction tank with a heating function with the help of a robotic arm; after keeping warm at a temperature of 50°C for 5min (the reaction time is defined as t), start the liquid pump 3, draw 50mL of DNS solution from the color development liquid tank into the reaction tank to obtain a mixed solution with a total volume of V2; boil for 8min to cause color development, draw 10mL of solution from the reaction tank into the sample tank with the help of a robotic arm, and measure the absorbance A at 540nm. i ';

[0060] Calculation of enzyme concentration difference: Based on absorbance A i ' value, and compared with the standard curve, the immediate amylase activity a' value in the enzyme tank was calculated as follows: enzyme activity a' = C' × V2 / (V1 × t), where C' is 0.6 mg / mL from the standard curve, V2 is the total volume of the mixed solution in the reaction tank (2.5 + 50 + 50 = 102.5 mL), V1 is the volume of the enzyme solution aspirated from the enzyme tank (2.5 mL), t is 5 min, and the obtained enzyme activity a' value is 4.92 U / mL;

[0061] Calculation of high-concentration enzyme solution replenishment: Based on the difference between the calculated enzyme activity a' and the initial enzyme activity a in the initial enzyme solution tank, the required high-concentration enzyme solution addition amount V3 is calculated, V3 = (a-a') × V0 / a0, wherein a and a' are 5.20 and 4.92 U / mL respectively, V0 is 30 L, the enzyme activity concentration a0 in the high-concentration enzyme solution tank is 20 U / mL, and the calculated addition amount is 0.42 L; the enzyme addition pump is started through the main control board to draw a fixed amount of amylase enzyme solution from the high-concentration enzyme solution tank to the enzyme solution tank; steps (2) to (4) complete the rapid detection and addition process of amylase enzyme activity in the enzyme solution tank within 15 minutes.

[0062] Example 2

[0063] A method for rapid detection and addition of enzyme activity in enzymatic pretreatment of cotton fabrics based on the above system comprises the following steps:

[0064] (1) Drawing of the standard curve of enzymatic hydrolysis products

[0065] The standard curve was prepared using 3,5-dinitrosalicylic acid and D-galacturonic acid. Specifically, 10 g of 3,5-dinitrosalicylic acid, 200 g of potassium sodium tartrate, 10 g of caustic soda, 3 g of phenol, and 5 g of sodium sulfite were mixed and dissolved in water to a volume of 1000 mL to obtain DNS solution. 10 mL of concentration C i 10 mL of DNS solution was added to 0, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, and 1.0 mg / mL D-galacturonic acid solutions, respectively. The mixture was shaken and boiled for 8 minutes to develop color. After cooling to room temperature, the solution was diluted to 100 mL and the absorbance A was measured at 540 nm. i , and obtain the standard curve A corresponding to the absorbance and D-galacturonic acid concentration i =k×C i (where k is the molar absorptivity);

[0066] (2) Color development and determination of reaction solution

[0067] When starting the test, enter the target pectinase activity of 3.0 U / mL and the total volume of enzyme solution in the enzyme solution tank of 40 L in the user interface, start the liquid pump 1, draw 2.5 mL of enzyme solution with a volume V1 from the enzyme solution tank where the cotton fabric is padded, and inject it into the reaction tank with the help of a robotic arm; start the liquid pump 2, draw 50 mL of 30 g / L pectin solution from the substrate solution tank, and introduce it into the reaction tank with a heating function with the help of a robotic arm; after keeping it at a temperature of 50°C for 10 minutes (the reaction time is defined as t), start the liquid pump 3, draw 50 mL of DNS solution from the color development liquid tank into the reaction tank to obtain a mixed solution with a total volume of V2; boil for 8 minutes to cause color development, draw 10 mL of solution from the reaction tank into the sample tank with the help of a robotic arm, and measure the absorbance A at 540 nm. i ';

[0068] Calculation of enzyme concentration difference: Based on absorbance A i ' value, and compared with the standard curve, the immediate pectinase activity a' value in the enzyme tank was calculated as follows: enzyme activity a'=C'×V2 / (V1×t), where C' is 0.65 mg / mL from the standard curve, V2 is the total volume of the mixed solution in the reaction tank, which is 2.5+50+50=102.5 mL, V1 is the volume of the enzyme solution aspirated from the enzyme tank, which is 2.5 mL, and t is 10 min. The obtained enzyme activity a' value is 2.67 U / mL.

[0069] Calculation of high-concentration enzyme solution replenishment: Based on the difference between the calculated enzyme activity a' and the initial enzyme activity a in the initial enzyme solution tank, calculate the required high-concentration enzyme solution addition amount V3, V3 = (a-a') × V0 / a0, where a and a' are 3.0 and 2.67 U / mL respectively, V0 is 40 L, the enzyme activity concentration a0 in the high-concentration enzyme solution tank is 50 U / mL, and the addition amount is 0.264 L; start the enzyme addition pump through the main control panel to draw a fixed amount of pectinase enzyme solution from the high-concentration enzyme solution tank to the enzyme solution tank, completing a rapid pectinase enzyme activity detection and addition process within 20 minutes.

[0070] Comparative Example 1

[0071] The same enzymatic hydrolysis product standard curve of amylase as in Example 1 was used; manual operation and calculation methods were used to replace the enzyme solution absorption, substrate solution addition, DNS solution addition, absorbance measurement of the mixed solution after reaction, and high-concentration enzyme supplementation described in Example 1; throughout the entire process, the manual operation method was used to complete the detection and addition process of amylase activity in the enzyme solution tank within 45 minutes at the fastest; the cotton fabric and pretreatment equipment used in the treatment of Comparative Example 1 were the same as those in Example 1.

[0072] Comparative Example 2

[0073] The same pectinase hydrolysis product standard curve as in Example 2 was used; manual operation and calculation methods were used instead of the enzyme solution absorption, substrate solution addition, DNS solution addition, absorbance measurement of the mixed solution after reaction, and high-concentration enzyme supplementation described in Example 2; the detection and addition process of the pectinase activity in the enzyme solution tank was completed once within 65 minutes in the entire process; the cotton fabric and pretreatment equipment used in the treatment of Comparative Example 2 were the same as those in Example 2.

[0074] The semi-finished cotton fabrics obtained in Examples 1-2 and Comparative Examples 1-2 were subjected to performance tests. The starch content of the cotton fabrics was determined using the perchloric acid method (see Qian Shiru. Enzymatic Desizing of Cotton Fabrics and Its Mechanism of Action: Master's Thesis. Wuxi: Jiangnan University, 2014). The desizing rate of the starch slurry was calculated. A higher value and a smaller standard deviation indicate a better enzymatic desizing effect and a more uniform treatment.

[0075] Following pectinase treatment of cotton fabric, the surface wettability was measured according to FZ / T 0-1-71-2008. The wicking height of moisture on the surface of the fabric was recorded. Higher values and smaller standard deviations indicate better enzyme scouring results and more uniform treatment. The desizing rate and wicking height of the cotton fabric were measured at 20-minute intervals over a three-hour period at the drop end after enzymatic pretreatment. The average and standard deviation of the desizing rate and wicking height were calculated. The results are shown in Table 1.

[0076] Table 1 Desizing rate and wicking height of cotton fabric

[0077]

[0078]

[0079] From the results in Table 1, we can see that:

[0080] Compared with Comparative Example 1, the desizing rate of the cotton fabric obtained after treatment with the method of the present invention in Example 1 was higher and the standard deviation value was smaller, indicating that the method of the present invention improves the effect of enzymatic desizing of cotton fabric and the quality stability of semi-finished products by efficiently and quickly determining enzyme activity and timely supplementing high-concentration enzyme solution;

[0081] Compared with Comparative Example 2, the cotton fabric obtained after being treated with the method of the present invention in Example 2 has a higher wicking height and a smaller standard deviation value, indicating that the method of the present invention improves the enzyme scouring effect of cotton fabric and the quality stability of semi-finished products by efficiently and quickly determining the enzyme activity of pectinase in the enzyme tank and timely supplementing high-concentration pectinase;

[0082] In summary, in the enzymatic pretreatment of cotton fabrics, whether it is amylase desizing or pectinase scouring, the method described in the present invention improves the quality of cotton fabric pretreatment compared with the traditional method.

[0083] The embodiments provided above are not intended to limit the scope of the present invention, nor are the steps described to limit their execution order. Any obvious improvements to the present invention made by those skilled in the art in combination with existing common knowledge shall fall within the scope of protection defined by the claims of the present invention.

Claims

1. A system for rapid detection and addition of enzyme activity in enzymatic pretreatment of cotton fabrics, characterized in that: The system includes a user interface, a main control panel, an enzyme solution tank, a substrate solution tank, a reaction tank with a heating function, a color development solution tank, a high-concentration enzyme solution tank and a spectrophotometer; Among them, the reaction pool is used for the reaction and color development of enzyme solution and substrate solution; the enzyme solution tank is used to store enzyme solution for cotton fabric padding and is connected to the reaction pool through a liquid pump 1; the substrate solution tank is used to store substrate solution for enzyme reaction and is connected to the reaction pool through a liquid pump 2; the color development liquid tank is used to store solution for color development after the enzyme and substrate react and is connected to the reaction pool through a liquid pump 3; the high-concentration enzyme liquid tank is used to store high-concentration enzyme solution for additional use and is connected to the enzyme solution tank through an enzyme addition pump; the spectrophotometer is used to measure the absorbance after the reaction pool develops color and transmits the collected absorbance to the main control board; The main control board is used to coordinate various parts of the system, collect system parameters, and use built-in formulas to calculate enzyme activity and the amount of high-concentration enzyme solution to be added; the user interface is used to display the current measured enzyme activity and set the target enzyme activity and total volume of the enzyme solution in the enzyme solution tank.

2. The system according to claim 1, wherein: It also includes a robotic arm for collecting, pipetting and placing liquids in the system, which is controlled by the main control board.

3. The system according to claim 1, wherein: The enzyme adding pump is controlled by the main control board to add the amount of high-concentration enzyme and injects it into the enzyme liquid tank.

4. The system according to claim 1, wherein: The built-in formulas include: Standard curve A corresponding to absorbance and aldose concentration i =k×C i , where k is the molar absorptivity; Calculation of enzyme concentration difference: Based on absorbance A i 'Value, the concentration of the corresponding hydrolyzate C is obtained by comparing the standard curve i 'mg / mL, on this basis, the enzyme activity a' value in the enzyme tank is calculated as: Enzyme activity a'=C'×V2 / (V1×t), where C'=A i ' / k, V2 is the total volume of the mixed solution in the reaction tank, V1 is the volume of the enzyme solution drawn from the enzyme tank, and t is the reaction time; Calculation of high-concentration enzyme solution replenishment: Based on the difference between the calculated enzyme activity a' and the initial enzyme activity a in the initial enzyme solution tank, calculate the required high-concentration enzyme solution addition amount V3: The amount of high-concentration enzyme solution added V3 = (a-a') × V0 / a0, where a0 is the enzyme activity concentration in the high-concentration enzyme solution tank and V0 is the total volume of the enzyme solution in the squeeze tank.

5. A method for rapid detection and addition of enzyme activity in pre-treatment of cotton fabrics based on any one of claims 1 to 4, characterized in that: The method comprises the following steps: (1) Drawing of the standard curve of enzymatic hydrolysis products The standard curve A was prepared using 3,5-dinitrosalicylic acid and aldose. i =k×C i , where k is the molar absorption coefficient, built into the main control board; (2) Color development and determination of reaction solution When starting the test, the target enzyme activity a U / mL of the enzyme tank and the total volume V0 of the enzyme solution in the enzyme tank are input into the user interface. The liquid pump 1 is started to draw a volume V1 of enzyme solution from the enzyme tank where the cotton fabric is padded, and is injected into the reaction tank with the help of a robotic arm. The liquid pump 2 is started to draw a substrate solution from the substrate solution tank and introduce it into the reaction tank with the help of a robotic arm. After keeping the solution at a temperature of 50-60°C for 5-10 minutes (the reaction time is defined as t), the liquid pump 3 is started to draw the DNS solution from the color development liquid tank into the reaction tank to obtain a mixed solution with a total volume of V2. The solution is boiled to produce a color reaction, and the solution is drawn from the reaction tank into the sample cell of the spectrophotometer with the help of a robotic arm, and the absorbance A is measured at 540nm. i '; Absorbance A i 'Transmit to the main control board for calculation; Calculation of enzyme concentration difference: Based on absorbance A i 'Value, the concentration of the corresponding hydrolyzate C is obtained by comparing the standard curve i 'mg / mL, on this basis, the enzyme activity a' value in the enzyme tank is calculated as: Enzyme activity a'=C'×V2 / (V1×t), where C'=A i ' / k, V2 is the total volume of the mixed solution in the reaction tank, V1 is the volume of the enzyme solution drawn from the enzyme tank, and t is the reaction time; Calculation of high-concentration enzyme solution replenishment: Based on the difference between the calculated enzyme activity a' and the initial enzyme activity a in the initial enzyme solution tank, calculate the required high-concentration enzyme solution addition amount V3: The amount of high-concentration enzyme solution added V3 = (a-a') × V0 / a0, where a0 is the enzyme activity concentration in the high-concentration enzyme solution tank and V0 is the total volume of the enzyme solution in the manifold tank; (3) Start the enzyme adding pump through the main control panel to draw a fixed amount of enzyme solution from the high-concentration enzyme solution tank into the enzyme solution tank, completing a rapid enzyme activity detection and addition process.

6. The method according to claim 5, characterized in that In the drawing of the standard curve in step (1), when detecting the enzymatic activity of amylase, the aldose is glucose; when detecting the enzymatic activity of pectinase, the aldose is D-galacturonic acid.

7. The method according to claim 5, characterized in that The standard curve construction process in step (1) is specifically as follows: Dissolve 3,5-dinitrosalicylic acid, potassium sodium tartrate, caustic soda, phenol and sodium sulfite in water to obtain DNS solution; prepare a series of concentrations C i aldose solution, and add DNS solution respectively, shake well and boil to develop color, cool to room temperature and make up to volume, measure absorbance A at 540nm i ; Standard curve A corresponding to absorbance and aldose concentration i =k×C i ; where k is the molar absorptivity.

8. The method according to claim 5, characterized in that The volume ratio of the enzyme solution, substrate solution and DNS solution in the reaction pool of step (2) is 2-5:1:

1.

9. The method according to claim 5, characterized in that In step (2), in the color development and determination of the reaction solution, when detecting the enzymatic activity of amylase, the substrate solution is a soluble starch solution; when detecting the enzymatic activity of pectinase, the substrate solution is a pectin solution.

10. Use of the system according to any one of claims 1 to 4 in textile processing.

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