Algae culture device and method for regulating and controlling concentration of carbon dioxide in water body

Abstract

The invention belongs to the technical field of algae culture, and particularly relates to an algae culture device and method for regulating and controlling the concentration of carbon dioxide in a water body. The device comprises a gas supply bottle group, a gas distribution platform, a gas mixing chamber, an algae laboratory culture container and a carbon dioxide concentration monitor which are connected in sequence, the gas supply bottle group comprises a carbon dioxide gas bottle and an air bottle, and an electric control overflow valve and a flow controller are installed in the gas distribution platform; the gas mixing chamber is sequentially divided into a spiral gas inlet area, a core disturbance area and a fine integration area in the gas flowing direction, wherein the core disturbance area is provided with spoilers and a reverse jet-flow device, and the fine integration area is provided with a honeycomb rectifier. The stable output of the DCO2 concentration is monitored and controlled in real time, and the stability of the DCO2 concentration in the algae culture process is ensured, so that the growth efficiency and biomass accumulation of the algae under the stable carbon dioxide condition are ensured.

Description

Technical Field

[0001] The present invention belongs to the field of algae cultivation, and in particular relates to an algae cultivation device and method for regulating the concentration of carbon dioxide in water. Background Art

[0002] During the algae cultivation process, dissolved carbon dioxide (DCO2) in water is a key factor in algae photosynthesis and growth. Changes in its concentration will directly affect the metabolic activity, growth rate and biomass accumulation of algae.

[0003] In existing laboratory algae cultivation technology, the concentration of dissolved carbon dioxide (DCO2) in water is controlled by adding buffers or adjusting DCO2 with acid or alkali. However, this method is difficult to achieve stable and precise regulation and cannot guarantee the stability of the cultivation system. At the same time, there is a lack of efficient monitoring and regulation methods in the laboratory, and the DCO2 concentration in the cultivation system often fluctuates greatly, further leading to an unstable algae growth environment. Therefore, an algae cultivation device and method for regulating the carbon dioxide concentration in water are proposed to solve the above problems. Summary of the Invention

[0004] To overcome the above-mentioned shortcomings of the prior art, the present invention provides an algae cultivation device and method for regulating the concentration of carbon dioxide in water. The present invention ensures the stability of the DCO2 concentration during the algae cultivation process by real-time monitoring and controlling the stable output of the DCO2 concentration.

[0005] To achieve one of the above purposes, the present invention adopts the following technical solutions:

[0006] An algae cultivation device for regulating the carbon dioxide concentration in water comprises a gas supply cylinder group, a gas distribution platform, a gas mixing chamber, an algae laboratory cultivation container and a carbon dioxide concentration monitor, which are connected in sequence. The gas supply cylinder group comprises a carbon dioxide cylinder and an air cylinder. The gas distribution platform is equipped with an electrically controlled overflow valve and a flow controller. The gas mixing chamber is sequentially divided into a spiral air inlet area, a core disturbance area with spoilers and reverse jets, and a fine integration area with a honeycomb rectifier along the direction of gas flow. The algae laboratory cultivation container is also connected to a light adjustment component. The carbon dioxide concentration monitor is connected to the adjustment control component via a controller.

[0007] Preferably, the electrically-controlled overflow valve includes a first electrically-controlled overflow valve and a second electrically-controlled overflow valve, which are used to preliminarily adjust the gas pressure and flow; the flow controller includes a first flow controller and a second flow controller, which can accurately control the flow of each gas; the outlet of the carbon dioxide cylinder is connected to the first electrically-controlled overflow valve and the first flow controller in sequence through a first pipeline, and the gas outlet of the first flow controller is connected to the gas inlet of the gas mixing chamber; the outlet of the air cylinder is connected to the second electrically-controlled overflow valve and the second flow controller through a second pipeline, and the gas outlet of the second flow controller is connected to the gas inlet of the gas mixing chamber.

[0008] Preferably, a spiral pipe is provided in the spiral air inlet area, and the gas flows along the spiral pipe here to produce a preliminary rotational mixing effect. The air inlet of the spiral pipe is connected to the first flow controller and the second flow controller; the spiral pipe is connected to the core disturbance area, and the core disturbance area is provided with rotatable spoilers and reverse jets in sequence along the air flow direction. The flow of gas is affected by the spoilers and reverse jets, forming a stronger vortex and shearing effect, further accelerating the mixing of the two gases; the outlet of the core disturbance area is connected to the fine integration area, and a honeycomb rectifier is provided at the outlet of the fine integration area, which can effectively cause turbulent shear and rotation of the mixed gas, thereby enhancing the mixing and rectification effect of the flow, and ensuring that the mixed gas flows stably and evenly at the outlet.

[0009] Preferably, the carbon dioxide concentration monitor includes an air chamber consisting of an upper cover body and a bottom box. A waterproof and breathable membrane is installed in the middle of the upper cover body to block water vapor in the external environment from entering the air chamber, thereby avoiding interference of water vapor on the CO2 concentration detection results; an infrared light source and an infrared thermopile gas sensor are installed in the bottom box, and the air inlet of the bottom box is connected to the air outlet of the algae laboratory culture container through a rubber hose, and the air outlet of the bottom box is connected to the atmosphere.

[0010] Preferably, the algae laboratory culture container is a conical flask with a perforated silicone stopper, which is used to contain algae culture solution and for ventilation.

[0011] Preferably, the light adjustment component is a light lamp with adjustable light intensity.

[0012] Preferably, the algae laboratory culture container is placed in a constant temperature room with air conditioning.

[0013] Preferably, the gas in the carbon dioxide cylinder is carbon dioxide with a purity of ≥99.9%; the gas in the air cylinder is composed of 78%±1% nitrogen and 21%±1% oxygen.

[0014] Preferably, the gas outlet of the gas mixing chamber is connected to the gas inlet of the flow rate controller through a third pipe, and the gas outlet of the flow rate controller is connected to the gas inlet of the algae laboratory culture container through a fourth pipe.

[0015] To achieve the second objective above, the present invention provides an algae cultivation method for regulating carbon dioxide concentration in water, comprising the following steps:

[0016] S1. Inoculate algae into an algae laboratory culture container and add an appropriate amount of culture medium;

[0017] S2. Start the gas supply cylinder group and control the gas supply rate through the gas distribution platform to ensure that the DCO2 concentration in the water is maintained at the concentration set before the experiment;

[0018] S3, start the carbon dioxide concentration monitor to monitor the DCO2 concentration in the water in real time;

[0019] S4. Based on the feedback data from the carbon dioxide concentration monitor, the controller adjusts the CO2 supply and then accurately controls the mixed gas through the segmented gas mixing chamber to ensure that the DCO2 concentration is stable within the set range;

[0020] S5. Start the lighting system and temperature control system to provide appropriate lighting and temperature conditions to promote algae growth. The lighting system is the lighting system in the device, and the temperature control system is the constant temperature chamber equipped with air conditioning.

[0021] S6. Regularly monitor algae growth and adjust culture parameters as needed.

[0022] The advantages of the present invention are:

[0023] (1) The present invention ensures the stability of DCO2 concentration during algae cultivation by real-time monitoring and controlling the stable output of DCO2 concentration, thereby ensuring the growth efficiency and biomass accumulation of algae under stable carbon dioxide conditions. It has a simple structure and is easy to operate, making it suitable for small-scale algae cultivation in the laboratory.

[0024] (2) The device of the present invention includes multiple gas cylinders, two electrically controlled overflow valves, an algae culture area and a carbon dioxide concentration monitoring device. The electrically controlled overflow valve cooperates with the carbon dioxide concentration monitoring device to monitor the algae culture environment in real time. The device supplies carbon dioxide gas through the gas cylinder, and the carbon dioxide is transported to the algae culture area (algae laboratory culture container) after gas mixing. The gas flow and carbon dioxide concentration are precisely controlled by the electrically controlled overflow valve, and the two gases are precisely mixed through the gas mixing chamber to ensure that the carbon dioxide concentration remains within the preset range. The carbon dioxide concentration in the algae culture area is detected in real time by the carbon dioxide concentration monitoring device, and the monitoring data is fed back to the gas distribution platform through the controller to automatically adjust the gas flow so that the carbon dioxide concentration in the algae culture area is always maintained stable. The device effectively avoids the problem of large fluctuations in carbon dioxide concentration during the algae culture process, thereby ensuring the stability of the algae growth environment and improving the growth rate and yield of algae. This method can accurately control the concentration of dissolved carbon dioxide in the water body and ensure the growth of algae. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 Schematic diagram of the structure of the present invention Figure 1 .

[0026] Figure 2 Schematic diagram of the structure of the present invention Figure 2 .

[0027] Figure 3 This is a stability test diagram of Example 1 of the present invention.

[0028] Figure 4 The stability test diagram of Example 2 of the present invention.

[0029] The meanings of the symbols in the figure are as follows:

[0030] 1-Gas supply cylinder group, 2-Carbon dioxide cylinder, 3-Air cylinder, 4-Gas distribution platform, 5-First electronically controlled overflow valve, 6-First flow controller, 7-Second electronically controlled overflow valve, 8-Second flow controller, 9-Gas mixing chamber, 10-Spiral air inlet area, 11-Core disturbance area, 12-Fine integration area, 13-Flow rate controller, 14-Algae laboratory culture container, 15-Carbon dioxide concentration monitor, 16-Light adjustment component. DETAILED DESCRIPTION

[0031] like Figure 1-2As shown, an algae cultivation device for regulating the carbon dioxide concentration in water includes a gas supply cylinder group 1 (carbon dioxide cylinder 2 and air cylinder 3), a gas distribution platform 4, an algae cultivation area and a carbon dioxide concentration monitor 15. An electric-controlled overflow valve and a flow controller are installed inside the gas distribution platform; the algae cultivation area is an algae laboratory cultivation container 14, which can be a conical flask with a perforated silicone stopper for containing algae culture solution and ventilation.

[0032] Specifically, the side of the gas distribution platform 4 is connected to the outlet of the gas supply cylinder assembly 1 via a pipeline. The gas distribution platform 4 is equipped with a first electrically controlled relief valve 5, a second electrically controlled relief valve 7, a first flow controller 6, and a second flow controller 8. The outlet of the carbon dioxide cylinder 2 is connected to the first electrically controlled relief valve 5 and the first flow controller 6 in sequence via a first pipeline. The outlet of the first flow controller 6 is connected to the inlet of the gas mixing chamber 9. The outlet of the air cylinder 3 is connected to the second electrically controlled relief valve 7 and the second flow controller 8 via a second pipeline. The outlet of the second flow controller 8 is connected to the inlet of the gas mixing chamber 9. The flow controllers and the gas mixing chamber 9 work together to ensure a stable output of carbon dioxide concentration. Carbon dioxide and air enter the system through the cylinders. The flow rate and flow rate of each gas channel are monitored in real time by the flow controller and flow rate controller to ensure that the gases enter the gas mixing chamber 9 according to the set flow ratio.

[0033] The gas mixing chamber 9 is divided into a spiral air inlet area 10, a core disturbance area 11 with spoilers and reverse jets, and a fine integration area 12 with a honeycomb rectifier in the direction of gas flow; the algae culture container is also connected to the light adjustment component 16; the carbon dioxide concentration monitor 15 is connected to the adjustment control component through a controller. Each section in the segmented gas mixing chamber 9 can independently adjust parameters (such as gas flow, pressure, temperature, disturbance intensity, etc.). This adjustability allows optimization at different stages according to different needs. By dividing the gas chamber into multiple areas, each section can adopt different disturbance methods and flow patterns in a targeted manner. In addition, the segmented design allows the system to be flexibly adjusted according to actual needs and can adapt to changing working conditions such as different gases, pressures, and flows.

[0034] After the two streams of gas enter the gas mixing chamber 9, they first enter the spiral air inlet area 10, where the gas flows along the spiral pipe, producing a preliminary rotating mixing effect. Then, the mixed gas enters the core disturbance area 11. In this section, the gas flow is affected by the turbulence blades and the reverse jet device, forming stronger vortexes and shearing effects, further accelerating the mixing of the two gases. Finally, the gas flows to the fine integration area 12 before the outlet. A honeycomb rectifier is provided at the outlet to ensure that the mixed gas flows stably and evenly at the outlet. The gas mixing chamber 9 adopts segmented control, and each section can independently adjust the gas flow rate and disturbance intensity to ensure the adjustability and efficiency of the mixing process.

[0035] The outlet of the gas mixing chamber 9 is connected to the inlet of a flow controller 13 via a third conduit. This outlet is then connected to the inlet of an algae laboratory culture vessel 14 via a fourth conduit. The flow controller 13 ensures that gas enters the algae culture area at a constant flow rate, maintaining a constant DCO2 concentration within the culture system. The regulated gas is ultimately delivered to the algae culture area via the flow controller 13, ensuring that the CO2 concentration remains within the desired range and preventing concentration fluctuations that could affect algae growth.

[0036] Furthermore, the carbon dioxide concentration monitor 15 includes an air chamber consisting of an upper cover and a bottom box. A waterproof, breathable membrane is installed in the middle of the upper cover to prevent water vapor from the external environment from entering the air chamber, thereby preventing water vapor from interfering with the CO2 concentration detection results. An infrared light source and an infrared thermopile gas sensor are installed in the bottom box. The air inlet of the bottom box is connected to the air outlet of the algae laboratory culture container 14 via a rubber hose, and the air outlet of the bottom box is connected to the atmosphere. The carbon dioxide concentration monitor 15 is connected to the algae culture area and can monitor the CO2 concentration in the algae culture area in real time. The controller provides feedback to the electronically controlled overflow valve and flow controller. In addition, the light adjustment component 16 can stably control the light intensity to ensure that the algae in the experimental instrument grows in a steady-state environment.

[0037] The algae culture container is also connected to the light adjustment component 16 to provide suitable lighting conditions. The controller autonomously adjusts the length of day and night and the light intensity to promote the photosynthesis of algae and maintain a stable algae culture system. The light adjustment component 16 is specifically a light lamp with adjustable light intensity.

[0038] The algae laboratory culture container 14 is placed in a constant temperature room with air conditioning to maintain the culture solution within a suitable temperature range.

[0039] A method for cultivating algae for regulating carbon dioxide concentration in water, comprising the following steps:

[0040] Step 1: Inoculate algae into the algae laboratory culture container 14 and add an appropriate amount of culture medium.

[0041] Step 2: Start the gas supply cylinder group 1 and control the gas supply rate through the gas distribution platform 4 to ensure that the DCO2 concentration in the water is maintained at the concentration set before the experiment.

[0042] Step 3: Start the carbon dioxide concentration monitor 15 to monitor the DCO2 concentration in the water body in real time.

[0043] Step 4: Based on the feedback data from the carbon dioxide concentration monitor 15, the controller is used to adjust the CO2 supply and then control the mixed gas through the segmented gas mixing chamber 9 to ensure that the DCO2 concentration is stable within the set range.

[0044] Step 5: Start the lighting system and temperature control system to provide suitable light and temperature conditions to promote algae growth.

[0045] Step 6: Regularly monitor algae growth and adjust culture parameters as needed.

[0046] Example 1

[0047] The above device is used to culture cyanobacteria in the laboratory. The specific steps are as follows:

[0048] Step 1: Inoculate cyanobacteria into the algae laboratory culture container 14 and add culture medium.

[0049] Step 2: Start the gas supply cylinder group 1 and control the gas supply rate through the gas distribution platform 4 to ensure that the DCO2 concentration in the water is maintained at the concentration set before the experiment.

[0050] Step 3: Start the carbon dioxide concentration monitor 15 to monitor the DCO2 concentration in the water body in real time.

[0051] Step 4: Based on the feedback data from the carbon dioxide concentration monitor 15, the controller is used to adjust the CO2 supply and then control the mixed gas through the segmented gas mixing chamber 9 to ensure that the DCO2 concentration is stable within the set range.

[0052] Step 5: Start the lighting system and temperature control system to provide suitable light and temperature conditions to promote algae growth.

[0053] Step 6: Regularly monitor algae growth and adjust culture parameters as needed.

[0054] Example 2

[0055] The above device is used to culture green algae in the laboratory. The specific steps are as follows:

[0056] Step 1: Inoculate green algae into the algae laboratory culture container 14 and add culture medium.

[0057] Step 2: Start the gas supply cylinder group 1 and control the gas supply rate through the gas distribution platform 4 to ensure that the DCO2 concentration in the water is maintained at the concentration set before the experiment.

[0058] Step 3: Start the carbon dioxide concentration monitor 15 to monitor the DCO2 concentration in the water body in real time.

[0059] Step 4: Based on the feedback data from the carbon dioxide concentration monitor 15, the controller is used to adjust the CO2 supply and then control the mixed gas through the segmented gas mixing chamber 9 to ensure that the DCO2 concentration is stable within the set range.

[0060] Step 5: Start the lighting system and temperature control system to provide suitable light and temperature conditions to promote algae growth.

[0061] Step 6: Regularly monitor algae growth and adjust culture parameters as needed.

[0062] In the experiments of Example 1 and Example 2, the time-varying curve of dissolved carbon dioxide in water is as follows: Figure 3-4 As shown, it can be seen that the dissolved carbon dioxide in the water of the algae cultured using the device tends to be stable after 4 days, indicating that the regulation effect of the device is significant.

[0063] The present invention has the advantages of being able to stably control the DCO2 of water in a culture bottle, and solves the problem that existing carbon dioxide gas concentration control devices cause unstable concentration control when adjusting carbon dioxide concentration, thereby affecting experimental results. The purpose is to stably control the concentration of dissolved carbon dioxide in water by producing arbitrary carbon dioxide.

[0064] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. An algae cultivation device for regulating carbon dioxide concentration in water, characterized by: The invention comprises a gas supply cylinder group (1), a gas distribution platform (4), a gas mixing chamber (9), an algae laboratory culture container (14) and a carbon dioxide concentration monitor (15) connected in sequence. The gas supply cylinder group (1) comprises a carbon dioxide gas cylinder (2) and an air gas cylinder (3). An electric-controlled overflow valve and a flow controller are installed in the gas distribution platform (4). The gas mixing chamber (9) is sequentially divided into a spiral air inlet area (10), a core disturbance area (11) provided with a spoiler and a reverse jet, and a fine integration area (12) provided with a honeycomb rectifier along the gas flow direction. The algae laboratory culture container (14) is also connected to a light adjustment component (16). The carbon dioxide concentration monitor (15) is connected to the adjustment control component via a controller.

2. The algae cultivation device for regulating carbon dioxide concentration in water according to claim 1, characterized in that: The electrically controlled overflow valve comprises a first electrically controlled overflow valve (5) and a second electrically controlled overflow valve (7); the flow controller comprises a first flow controller (6) and a second flow controller (8); the outlet of the carbon dioxide cylinder (2) is connected to the first electrically controlled overflow valve (5) and the first flow controller (6) in sequence through a first pipeline, and the gas outlet of the first flow controller (6) is connected to the gas inlet of a gas mixing chamber (9); the outlet of the air cylinder (3) is connected to the second electrically controlled overflow valve (7) and the second flow controller (8) through a second pipeline, and the gas outlet of the second flow controller (8) is connected to the gas inlet of the gas mixing chamber (9).

3. The algae cultivation device for regulating carbon dioxide concentration in water according to claim 2, characterized in that: A spiral pipe is provided in the spiral air inlet area (10), and the air inlet of the spiral pipe is connected to the first flow controller (6) and the second flow controller (8); the spiral pipe is connected to the core disturbance area (11), and the core disturbance area (11) is provided with rotatable spoilers and reverse jets in sequence along the airflow direction; the outlet of the core disturbance area (11) is connected to the fine integration area (12), and a honeycomb rectifier is provided at the outlet of the fine integration area (12).

4. The algae cultivation device for regulating carbon dioxide concentration in water according to claim 1, characterized in that: The carbon dioxide concentration monitor (15) comprises an air chamber consisting of an upper cover and a bottom box, wherein a waterproof and breathable membrane is installed in the middle of the upper cover; an infrared light source and an infrared thermopile gas sensor are installed in the bottom box, and an air inlet of the bottom box is connected to an air outlet of an algae laboratory culture container (14) through a rubber hose, and the air outlet of the bottom box is connected to the atmosphere.

5. The algae cultivation device for regulating carbon dioxide concentration in water according to claim 1, characterized in that: The algae laboratory culture container (14) is a conical flask with a silica gel plug with holes.

6. The algae cultivation device for regulating carbon dioxide concentration in water according to claim 1, characterized in that: The light adjustment component (16) is a light lamp with adjustable light intensity.

7. The algae cultivation device for regulating carbon dioxide concentration in water according to claim 1, characterized in that: The algae laboratory culture container (14) is placed in a constant temperature room equipped with air conditioning.

8. The algae cultivation device for regulating carbon dioxide concentration in water according to claim 1, characterized in that: The gas in the carbon dioxide cylinder (2) is carbon dioxide with a purity of ≥99.9%; the gas in the air cylinder (3) is composed of 78%±1% nitrogen and 21%±1% oxygen.

9. The algae cultivation device for regulating carbon dioxide concentration in water according to claim 1, characterized in that: The gas outlet of the gas mixing chamber (9) is connected to the gas inlet of the flow rate controller (13) through a third pipe, and the gas outlet of the flow rate controller (13) is connected to the gas inlet of the algae laboratory culture container (14) through a fourth pipe.

10. An algae cultivation method for regulating carbon dioxide concentration in water according to any one of claims 1 to 9, characterized in that: The steps include: S1. Inoculate algae into an algae laboratory culture container (14) and add culture medium; S2, starting the gas supply cylinder group (1) and controlling the gas supply rate through the gas distribution platform (4); S3, start the carbon dioxide concentration monitor (15) to monitor the DCO2 concentration in the water body in real time; S4. Based on the feedback data from the carbon dioxide concentration monitor (15), the controller is used to adjust the CO2 supply and then control the mixed gas through the segmented gas mixing chamber (9); S5. Start the lighting system and temperature control system to provide appropriate lighting and temperature conditions; S6. Regularly monitor algae growth and adjust culture parameters as needed.

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