High-throughput separation culture device and method for intestinal flora

Abstract

The invention relates to the technical field of intestinal flora culture, in particular to an intestinal flora high-flux separation culture device and a method thereof.The intestinal flora high-flux separation culture device comprises a box, a plurality of detectors, a placement plate and a vibration mechanism, the vibration mechanism comprises a driving assembly, two driving shafts, four cams and three limiting assemblies, and each limiting assembly comprises two mounting blocks and a guide column; the multiple detectors are fixedly connected with the box body, one ends of the two driving shafts are arranged at the output end of the driving assembly, the other ends of the two driving shafts are rotationally connected with the box body, the four cams are fixedly connected with the two driving shafts respectively, the three limiting assemblies are arranged between the containing plate and the box body, and the two mounting blocks are fixedly connected with the box body. The two ends of the guide column are fixedly connected with the two mounting blocks respectively, intestinal flora in the multiple test tubes can be uniformly vibrated through the vibration mechanism, manual operation of an operator is not needed, a large amount of time is saved, and then the culture efficiency of the intestinal flora is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of intestinal flora cultivation, and in particular to a high-throughput separation and cultivation device for intestinal flora and a method thereof. Background Art

[0002] High-throughput intestinal flora isolation and culture devices are automated devices used to efficiently separate, culture, and analyze intestinal microorganisms. Currently, commercially available incubators are typically made of metal, which can lead to disadvantages such as rapid heat dissipation and difficulty observing, resulting in inaccurate experimental data.

[0003] In a patent application with prior art publication number CN 205933797 U, an array-type high-throughput microbial separation and culture device is disclosed. A staff member can apply force to a shielding cloth by pulling the bottom rod by hand, and then pull the shielding cloth down to shield the front, rear, left and right sides of the main body. Different effects can be achieved by using shielding cloths with different functions. For example, a shielding cloth made of opaque material can be used to block light, and a shielding cloth made of thermal insulation fabric can be used to keep the main body warm while facilitating observation, thereby ensuring the accuracy of the experimental results data.

[0004] However, in the existing technology, the intestinal flora in the physiological saline in the test tube is easily unevenly distributed, and the operator is required to hold the test tube to shake the intestinal flora in the physiological saline evenly. The operation of shaking the test tube by hand is cumbersome and takes a lot of time, resulting in low intestinal flora culture efficiency. Summary of the Invention

[0005] The purpose of the present invention is to provide a high-throughput separation and culture device for intestinal flora and a method thereof, aiming to solve the problem in the prior art that the intestinal flora in the physiological saline in the test tube is easily unevenly distributed, requiring the operator to shake the intestinal flora in the physiological saline by holding the test tube evenly. However, the operation of shaking the test tube by hand is cumbersome and takes a lot of time, resulting in low intestinal flora culture efficiency.

[0006] To achieve the above-mentioned objectives, the present invention provides a high-throughput separation and culture device for intestinal flora and a method thereof, comprising a box body, multiple detectors, a placement plate and a vibration mechanism, the vibration mechanism comprising a driving assembly, two driving shafts, four cams and three limiting assemblies, the limiting assembly comprising two mounting blocks and a guide column, the multiple detectors are fixedly connected to the box body and are located at the bottom of the box body, the driving assembly is arranged on the box body, one end of the two driving shafts is arranged at the output end of the driving assembly, the other end of the two driving shafts is rotatably connected to the box body and is located in the box body, the four cams are respectively fixedly connected to the two driving shafts, and the placement plate is located above the four cams, the three limiting assemblies are arranged between the placement plate and the box body, the two mounting blocks are fixedly connected to the box body and are respectively on the upper and lower sides of the placement plate, the two ends of the guide column are respectively fixedly connected to the two mounting blocks, the guide column is slidably connected to the placement plate and passes through the placement plate.

[0007] Among them, the drive assembly includes a transmission box, a drive motor and a transmission kit, the transmission box is fixedly connected to one end of the box body, the drive motor is fixedly connected to the transmission box, one of the drive shafts is fixedly connected to the output end of the drive motor, and the transmission kit is arranged between the two drive shafts.

[0008] The transmission kit includes a driving wheel, a driven wheel and a belt. The driving wheel is fixedly connected to the driving shaft where the driving motor is located, and the driven wheel is fixedly connected to another driving shaft. The driving wheel and the driven wheel are connected through the belt transmission.

[0009] Wherein, the vibration mechanism further includes a cover plate and a clamping ring, the clamping ring is fixedly connected to the cover plate, and the clamping ring is slidably connected to the box body and is located above the box body.

[0010] Wherein, the vibration mechanism further includes an internal threaded barrel, an external threaded barrel and a sealing plate, the internal threaded barrel is fixedly connected to the placement plate, the external threaded barrel is fixedly connected to the sealing plate, and the internal threaded barrel is threadedly connected to the external threaded barrel.

[0011] Wherein, the high-throughput separation and culture device for intestinal flora further includes an observation component, one end of the box body is provided with an observation window, and the observation component is arranged at one end of the box body close to the observation window.

[0012] Among them, the observation assembly includes a fixed frame, a movable plate and a threaded rod, the movable plate has a threaded hole, the fixed frame is fixedly connected to the box body, and the observation window is located in the fixed frame, the movable plate is slidingly connected to the fixed frame, and the threaded rod is threadedly connected to one end of the fixed frame and extends into the threaded hole.

[0013] The present invention provides a high-throughput separation and culture device for intestinal flora and a method thereof. When culturing intestinal flora, a plurality of test tubes containing intestinal flora are fixed on the placement plate. When the intestinal flora in the test tubes is shaken, the drive assembly is started. The drive assembly controls the two drive shafts and the four cams to rotate simultaneously. Since the placement plate is tightly attached to the four cams, when the protruding parts of the four cams contact the placement plate, the placement plate is lifted up. When the protruding parts of the four cams leave the placement plate, the placement plate will fall. This reciprocating process causes the placement plate and the plurality of test tubes to vibrate, causing the intestinal flora in the test tubes to be shaken and evenly mixed until the intestinal flora is thoroughly shaken. Subsequently, the intestinal flora in the test tubes can be monitored in real time through the plurality of detectors. In this way, the intestinal flora in the plurality of test tubes can be quickly shaken and evenly mixed, and the operator does not need to shake the plurality of test tubes one by one, which greatly reduces the time spent and thereby improves the culture efficiency of the intestinal flora. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art.

[0015] Figure 1 Schematic diagram of the structure of the high-throughput separation and culture device for intestinal flora according to the first embodiment of the present invention.

[0016] Figure 2 4 is a cross-sectional view of a high-throughput separation and culture device for intestinal flora according to a first embodiment of the present invention.

[0017] Figure 3 It is a schematic structural diagram of the interior of the box body of the first embodiment of the present invention.

[0018] Figure 4 Schematic diagram of the structure of a high-throughput separation and culture device for intestinal flora according to the second embodiment of the present invention.

[0019] Figure 5 2 is a schematic structural diagram of a movable plate according to a second embodiment of the present invention.

[0020] Figure 6 It is a flow chart of the steps of a high-throughput separation and culture method for intestinal flora of the present invention.

[0021] 101-box, 102-transmission box, 103-drive motor, 104-drive shaft, 105-driving pulley, 106-driven pulley, 107-belt, 108-cam, 109-placement plate, 110-mounting block, 111-guide column, 112-inner threaded cylinder, 113-external threaded cylinder, 114-sealing plate, 115-retaining ring, 116-cover plate, 117-detector, 201-observation window, 202-fixed frame, 203-movable plate, 204-threaded rod, 205-threaded hole. DETAILED DESCRIPTION

[0022] The first embodiment of this application is:

[0023] See also Figures 1 to 3 ,in, Figure 1 Schematic diagram of the structure of the high-throughput separation and cultivation device for intestinal flora according to the first embodiment of the present invention. Figure 2 is a cross-sectional view of a high-throughput separation and culture device for intestinal flora according to a first embodiment of the present invention. Figure 3 It is a schematic structural diagram of the interior of the box body of the first embodiment of the present invention.

[0024] The present invention provides a high-throughput separation and culture device for intestinal flora, comprising a housing 101, multiple detectors 117, a placement plate 109 and a vibration mechanism, wherein the vibration mechanism comprises a threaded barrel, an externally threaded barrel 113, a sealing plate 114, a drive assembly, a cover plate, a retaining ring 115, two drive shafts 104, four cams 108 and three limit assemblies, wherein the limit assembly comprises two mounting blocks 110 and a guide column 111, the drive assembly comprises a transmission box 102, a drive motor 103 and a transmission kit, wherein the transmission kit comprises a driving wheel 105, a driven wheel 106 and a belt 107. The above-mentioned solution solves the problem in the prior art that the intestinal flora in the physiological saline in the test tube is easily unevenly distributed, requiring the operator to hold the test tube to shake the intestinal flora in the physiological saline evenly, and the operation of shaking the test tube by hand is cumbersome and takes a lot of time, resulting in low culture efficiency of the intestinal flora.

[0025] In this embodiment, the multiple detectors 117 are fixedly connected to the box body 101 and are located at the bottom of the box body 101. The driving assembly is arranged on the box body 101. One end of the two driving shafts 104 is arranged at the output end of the driving assembly. The other end of the two driving shafts 104 is rotatably connected to the box body 101 and is located in the box body 101. The four cams 108 are respectively fixedly connected to the two driving shafts 104, and the placement plate 109 is located above the four cams 108. The three limiting assemblies are arranged between the placement plate 109 and the box body 101. The two mounting blocks 110 are fixedly connected to the box body 101 and are respectively on the upper and lower sides of the placement plate 109. The two ends of the guide column 111 are respectively fixedly connected to the two mounting blocks 110. The guide column 111 is slidably connected to the placement plate 109 and passes through the placement plate 109. When culturing intestinal flora, the intestinal flora is placed Many test tubes are fixed on the placement plate 109. When the intestinal flora in the test tubes is shaken, the driving component is started, and the driving component controls the two driving shafts 104 and the four cams 108 to rotate simultaneously. Since the placement plate 109 is tightly attached to the four cams 108, when the protruding parts of the four cams 108 contact the placement plate 109, the placement plate 109 is lifted up, and when the protruding parts of the four cams 108 leave the placement plate 109, the placement plate 109 will fall. This reciprocating process makes the placement plate 109 and many test tubes vibrate, so that the intestinal flora in the test tubes is shaken and evenly mixed until the intestinal flora is thoroughly shaken. Subsequently, the intestinal flora in the test tubes can be monitored in real time through the multiple detectors 117. In this way, the intestinal flora in many test tubes can be quickly shaken and evenly mixed, and the operator does not need to shake the many test tubes one by one, which greatly reduces the time spent and thus improves the cultivation efficiency of the intestinal flora.

[0026] Furthermore, the transmission box 102 is fixedly connected to one end of the box body 101, the drive motor 103 is fixedly connected to the transmission box 102, one of the drive shafts 104 is fixedly connected to the output end of the drive motor 103, and the transmission kit is arranged between the two drive shafts 104.

[0027] Furthermore, the driving wheel 105 is fixedly connected to the driving shaft 104 where the driving motor 103 is located, the driven wheel 106 is fixedly connected to another driving shaft 104 , and the driving wheel 105 and the driven wheel 106 are connected through the belt 107 .

[0028] In this embodiment, when the present invention is used to culture intestinal flora, a plurality of test tubes containing intestinal flora are fixed on the placement plate 109. When the intestinal flora in the test tubes are shaken, the drive motor 103 is started, and the drive motor 103 drives one of the drive shafts 104 to rotate. Through the transmission of the active wheel 105, the driven wheel 106 and the belt 107, the other drive shaft 104 rotates at the same time. The drive motor 103 controls the two drive shafts 104 and the four cams 108 to rotate at the same time. Since the placement plate 109 is tightly attached to the four cams 108, when the protruding parts of the four cams 108 are When it contacts the placement plate 109, the placement plate 109 is lifted up, and when the protruding parts of the four cams 108 leave the placement plate 109, the placement plate 109 will fall, and this reciprocating movement will make the placement plate 109 and the numerous test tubes vibrate, so that the intestinal flora in the test tubes are shaken and evenly mixed, until the intestinal flora are thoroughly shaken, and then the intestinal flora in the test tubes can be monitored in real time through the multiple detectors 117. In this way, the intestinal flora in many test tubes can be quickly shaken and evenly mixed, and the operator does not need to shake the numerous test tubes one by one, which greatly reduces the time spent and thus improves the cultivation efficiency of the intestinal flora.

[0029] Furthermore, the snap ring 115 is fixedly connected to the cover plate, and the snap ring 115 is slidably connected to the box body 101 and is located above the box body 101 .

[0030] In this embodiment, when installing the test tube, the top of the box 101 is in an open state. After the test tube is installed, the clamping ring 115 is slid into the box 101, and the cover closes the top of the box 101 to facilitate the operator to put in and take out the test tube.

[0031] Furthermore, the internal threaded barrel 112 is fixedly connected to the placement plate 109 , the external threaded barrel 113 is fixedly connected to the sealing plate 114 , and the internal threaded barrel 112 is threadedly connected to the external threaded barrel 113 .

[0032] In this embodiment, after the test tube is installed, the external threaded tube 113 is threadedly connected to the internal threaded tube 112 so that the sealing plate 114 seals the top of the test tube. At the same time, the test tube is clamped between the placement plate 109 and the sealing plate 114 to ensure that the test tube will not be misplaced when vibrating.

[0033] When the intestinal flora is cultivated using the present invention, a plurality of test tubes containing intestinal flora are fixed on the placement plate 109. When the intestinal flora in the test tubes is shaken, the drive motor 103 is started. The drive motor 103 drives one of the drive shafts 104 to rotate. Through the transmission of the active wheel 105, the driven wheel 106 and the belt 107, the other drive shaft 104 rotates at the same time. The drive motor 103 controls the two drive shafts 104 and the four cams 108 to rotate at the same time. Since the placement plate 109 is tightly attached to the four cams 108, when the protruding parts of the four cams 108 touch the When the placement plate 109 is placed, the placement plate 109 is lifted up, and when the protruding parts of the four cams 108 leave the placement plate 109, the placement plate 109 will fall, and this reciprocating movement will make the placement plate 109 and the numerous test tubes vibrate, so that the intestinal flora in the test tubes are shaken and evenly mixed, until the intestinal flora are thoroughly shaken, and then the intestinal flora in the test tubes can be monitored in real time through the multiple detectors 117. In this way, the intestinal flora in numerous test tubes can be quickly shaken and evenly mixed, and the operator does not need to shake the numerous test tubes one by one, which greatly reduces the time spent and thus improves the cultivation efficiency of the intestinal flora.

[0034] The second embodiment of this application is:

[0035] Based on the first embodiment, based on the first embodiment, please refer to Figures 4 and 5 , Figure 4 Schematic diagram of the structure of a high-throughput separation and cultivation device for intestinal flora according to the second embodiment of the present invention. Figure 5 FIG. 2 is a schematic structural diagram of the movable plate 203 according to the second embodiment of the present invention.

[0036] The present invention provides a high-throughput separation and culture device for intestinal flora, which also includes an observation component. One end of the box 101 has an observation window 201. The observation component includes a fixed frame 202, a movable plate 203 and a threaded rod 204. The movable plate 203 has a threaded hole 205. Through the above scheme, the operator can observe the situation inside the box 101, thereby improving convenience.

[0037] In this embodiment, one end of the box body 101 has an observation window 201, the observation assembly is arranged at one end of the box body 101 close to the observation window 201, the fixed frame 202 is fixedly connected to the box body 101, and the observation window 201 is located in the fixed frame 202, the movable plate 203 is slidably connected to the fixed frame 202, the threaded rod 204 is threadedly connected to one end of the fixed frame 202 and extends into the threaded hole 205, when the operator observes the situation in the box body 101, he / she looks up Slide the movable plate 203 so that the threaded hole 205 on the movable plate 203 is aligned with the threaded rod 204. When the threaded rod 204 is rotated into the threaded hole 205, the observation window 201 is opened, and the operator can observe the situation inside the box 101 through the observation window 201. When the observation window 201 needs to be closed, the movable plate 203 is moved downward to close the observation window 201. In this way, the operator can easily observe the situation inside the box 101, which improves convenience.

[0038] The third embodiment of this application is:

[0039] Based on the second embodiment, please refer to Figure 6 , Figure 6 It is a flow chart of the steps of a high-throughput separation and culture method for intestinal flora of the present invention.

[0040] The present invention provides a high-throughput separation and culture method for intestinal flora, comprising the following steps:

[0041] S1: Fixing a plurality of test tubes containing intestinal flora on the placement plate 109;

[0042] S2: After the test tube is installed, the external threaded barrel 113 is threadedly connected to the internal threaded barrel 112 so that the sealing plate 114 seals the top of the test tube. At the same time, the test tube is clamped between the placement plate 109 and the sealing plate 114 to ensure that the test tube will not be misaligned when vibrating.

[0043] S3: After the sealing plate 114 is installed, the snap ring 115 is slid into the box body 101, and the cover plate closes the top of the box body 101;

[0044] S4: Starting the driving motor 103, the driving motor 103 drives the two driving shafts 104 and the four cams 108 to rotate simultaneously;

[0045] S5: Using the four cams 108 to drive the placement plate 109 to move up and down, so as to shake the intestinal flora in the test tube until the intestinal flora is thoroughly shaken;

[0046] S6: The multiple detectors 117 can be used to monitor the intestinal flora in the test tube in real time.

[0047] When using the high-throughput separation and culture method of intestinal flora of this embodiment, a plurality of test tubes containing intestinal flora are fixed on the placement plate 109. After the test tubes are installed, the external threaded cylinder 113 is threadedly connected to the internal threaded cylinder 112 so that the sealing plate 114 seals the top of the test tube. At the same time, the test tube is clamped between the placement plate 109 and the sealing plate 114 to ensure that the test tube will not be misaligned when vibrating. After the sealing plate 114 is installed, the retaining ring 115 is slid into the box 101, and the cover plate seals the top of the box 101. The drive motor 103 is started, and the drive motor 103 drives the two drive shafts 104 and the four cams 108 to rotate simultaneously. The four cams 108 drive the placement plate 109 to move up and down, so that the intestinal flora in the test tube is shaken and evenly mixed until the intestinal flora is thoroughly mixed. Then, the intestinal flora in the test tube can be monitored in real time through the multiple detectors 117.

[0048] The above disclosure is only a preferred embodiment of the present application and cannot be used to limit the scope of rights of the present application. Ordinary technicians in this field can understand that all or part of the processes of the above embodiment and equivalent changes made in accordance with the claims of the present application are still within the scope covered by the present application.

Claims

1. A high-throughput separation and culture device for intestinal flora, characterized in that: The cam is fixedly connected to the housing and the positioning plate is located above the cams. The three limit assemblies are arranged between the placement plate and the housing. The two mounting blocks are fixedly connected to the housing and are located at the upper and lower sides of the placement plate. The two ends of the guide column are fixedly connected to the two mounting blocks respectively. The guide column is slidably connected to the placement plate and passes through the placement plate.

2. The high-throughput separation and culture device for intestinal flora according to claim 1, characterized in that: The drive assembly includes a transmission box, a drive motor and a transmission kit. The transmission box is fixedly connected to one end of the box body, the drive motor is fixedly connected to the transmission box, one of the drive shafts is fixedly connected to the output end of the drive motor, and the transmission kit is arranged between the two drive shafts.

3. The high-throughput separation and culture device for intestinal flora according to claim 2, characterized in that: The transmission kit includes a driving wheel, a driven wheel and a belt. The driving wheel is fixedly connected to the driving shaft where the driving motor is located. The driven wheel is fixedly connected to another driving shaft. The driving wheel and the driven wheel are connected through the belt transmission.

4. The high-throughput separation and culture device for intestinal flora according to claim 3, characterized in that: The vibration mechanism further includes a cover plate and a snap ring, wherein the snap ring is fixedly connected to the cover plate, and the snap ring is slidably connected to the box body and is located above the box body.

5. The high-throughput separation and culture device for intestinal flora and the method thereof according to claim 4, characterized in that: The vibration mechanism further includes an internal threaded barrel, an external threaded barrel and a sealing plate. The internal threaded barrel is fixedly connected to the placement plate, the external threaded barrel is fixedly connected to the sealing plate, and the internal threaded barrel is threadedly connected to the external threaded barrel.

6. The high-throughput separation and culture device for intestinal flora according to claim 5, characterized in that: The high-throughput separation and culture device for intestinal flora further includes an observation component. One end of the box body is provided with an observation window, and the observation component is arranged at one end of the box body close to the observation window.

7. The high-throughput separation and culture device for intestinal flora according to claim 6, characterized in that: The observation assembly includes a fixed frame, a movable plate and a threaded rod, the movable plate has a threaded hole, the fixed frame is fixedly connected to the box body, and the observation window is located in the fixed frame, the movable plate is slidingly connected to the fixed frame, and the threaded rod is threadedly connected to one end of the fixed frame and extends into the threaded hole.

8. A high-throughput separation and culture method for intestinal flora, applied to the high-throughput separation and culture device for intestinal flora according to claim 7, characterized in that: The steps include: fixing a plurality of test tubes containing intestinal flora on the placement plate; After the test tube is installed, the external threaded barrel is threadedly connected to the internal threaded barrel so that the sealing plate seals the top of the test tube. At the same time, the test tube is clamped between the placement plate and the sealing plate to ensure that the test tube will not be dislocated when vibrating. After the sealing plate is installed, the snap ring is slid into the box body, and the cover plate closes the top of the box body; Starting the drive motor, the drive motor drives the two drive shafts and the four cams to rotate simultaneously; The four cams are used to drive the placement plate to move up and down, so as to shake the intestinal flora in the test tube until the intestinal flora is thoroughly shaken; Real-time monitoring of the intestinal flora in the test tube can be achieved by using a plurality of the detectors.

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