A method for enhancing the efficiency of long-distance transport of sea urchins based on dry transport and anhydrous volatile anesthesia

Abstract

The present invention relates to the technical field of live aquatic animal transportation, and discloses a method for enhancing the long-distance transportation of sea urchins based on dry transportation and waterless volatile anesthesia. The method comprises the following steps: placing the sea urchin in a sealed container, using a natural volatile substance as an anesthetic, loading the anesthetic with an adsorption material and volatilizing the anesthetic, and performing waterless anesthesia on the sea urchin; placing the anesthetized sea urchin upside down on a fixed bracket with a mesh structure in the transport container with its mouth facing upward and its spines facing downward, and utilizing the self-locking effect of the spines and the mesh to prevent the sea urchin from rolling during transportation; providing a functional interlayer in the fixed bracket, and placing a temperature control material and a moisturizing material in the functional interlayer to maintain the temperature and humidity of the transportation environment; and placing the sea urchin in seawater containing a cactus extract for initial recovery after transportation, and then transferring the sea urchin to flowing natural seawater for unified recovery. The present invention can construct a full-process collaborative protection chain for waterless anesthesia damage prevention, dry transportation steady-state maintenance, and rapid water phase repair, thereby significantly improving the survival rate and quality of dry transportation.

Description

Technical Field

[0001] The invention relates to the technical field of live aquatic animal transportation, in particular to a method for enhancing the efficiency of long-distance transportation of sea urchins based on dry transportation and anhydrous volatile anesthesia. Background Art

[0002] In the current aquaculture industry, sea urchins are marine organisms with high economic value. With the increasing consumption demand for fresh sea urchins in some inland cities, the demand for fresh sea urchins has been rising year by year, and the demand for their live transportation has been increasing.

[0003] The existing method of transporting sea urchins usually uses containers with ice packs for insulation, which can only meet the needs of short-term transportation. There is no comprehensive collaborative solution covering the entire process before transportation (anesthesia + fixation), during transportation (environmental stabilization), and after transportation (rapid repair). In particular, there is a lack of efficient and harmless physiological protection methods suitable for dry transportation mode. Summary of the Invention

[0004] The purpose of the present invention is to provide a method for enhancing the efficiency of long-distance transportation of sea urchins based on dry transportation and anhydrous volatile anesthesia, aiming to solve or improve at least one of the above-mentioned technical problems.

[0005] To achieve the above object, the present invention provides the following solution: The present invention provides a method for enhancing the efficiency of long-distance transport of sea urchins based on dry transport and anhydrous volatile anesthesia, comprising the following steps:

[0006] Anhydrous volatile anesthesia pretreatment: The sea urchin is placed in a sealed container and a natural volatile substance is used as an anesthetic. The volatile substance is loaded and evaporated through an adsorption material to perform anhydrous anesthesia on the sea urchin.

[0007] Inverted fixation to prevent damage: Place the anesthetized sea urchin upside down on a fixed support with a mesh structure in the transport container with the mouth facing up and the spines facing down. The self-locking effect between the spines and the mesh prevents the sea urchin from rolling over during transport.

[0008] Partitioned temperature control and buffering treatment: a functional interlayer is set in the fixed bracket, and temperature control materials and moisturizing materials are placed in the functional interlayer to maintain the transportation environment temperature at 4-15°C and relative humidity>90%;

[0009] Natural Extract Recovery Treatment: After transport, the sea urchins are first placed in seawater containing cactus extract for initial recovery, and then transferred to flowing natural seawater for complete recovery.

[0010] Optionally, the natural volatile substance is magnolia essential oil, the dosage is 0.01% of the sealed container, and the fumigation time is 30 minutes.

[0011] Optionally, the mesh diameter of the fixing bracket is smaller than the length of the sea urchin spine and larger than the diameter of the spine.

[0012] Optionally, the temperature control material is a biological ice bag.

[0013] Optionally, the moisturizing material is saturated water-containing sawdust.

[0014] Optionally, the salinity of the seawater containing the cactus extract is 32‰, and the concentration of the cactus extract is 30 ppm.

[0015] Optionally, the sea urchin is placed in seawater containing cactus extract for initial recovery, wherein the initial recovery immersion time is 0.5 hours.

[0016] Optionally, the fixing bracket is made of plastic or foam material.

[0017] Optionally, the transport container is made of foam material.

[0018] Optionally, the fixing bracket includes:

[0019] A plurality of spacers with a mesh structure, wherein the spacers are used to place sea urchins, and the functional interlayer is formed between two adjacent spacers;

[0020] A plurality of support frames are alternately arranged with the plurality of partition nets.

[0021] The present invention discloses the following technical effects:

[0022] The present invention uses natural volatile substances as anesthetics to replace traditional aqueous anesthesia. It does not require water and perfectly matches the waterless transportation scenario. It effectively reduces weight and saves the cost of liquid preparation and water replacement, achieving harmless and convenient stress control. It combines inverted fixation with zoned temperature control and buffering to minimize physical damage and maintain a stable microenvironment. It uses cactus extract recovery fluid to specifically repair the oxidative damage accumulated during transportation, and can construct a full-process collaborative protection chain of "waterless anesthesia damage prevention (before) → dry transportation steady-state maintenance (middle) → rapid aqueous phase repair (after)", which significantly improves the survival rate and quality of dry transportation. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] The accompanying drawings, which constitute part of this application, are intended to provide a further understanding of this application. The exemplary embodiments and descriptions of this application are intended to explain this application and do not constitute an improper limitation on this application. In the accompanying drawings:

[0024] Figure 1 This is a schematic structural diagram of a transport container according to the present invention;

[0025] Figure 2 Schematic diagram of the intestinal T-AOC activity test of the present invention;

[0026] Figure 3 Schematic diagram of the gonad T-AOC activity test of the present invention;

[0027] Figure 4 Schematic diagram of the intestinal GSH activity test of the present invention;

[0028] Figure 5 This is a schematic diagram of the gonad GSH activity test of the present invention;

[0029] Figure 6 This is a schematic diagram of the intestinal MDA activity test of the present invention;

[0030] Figure 7 This is a schematic diagram of the gonad MDA activity test of the present invention;

[0031] Figure 8 This is a schematic diagram of the intestinal SOD activity test of the present invention;

[0032] Figure 9 This is a schematic diagram of the gonad SOD activity test of the present invention;

[0033] In the figure: 1. Transport container; 2. Support frame; 3. Partition net. DETAILED DESCRIPTION

[0034] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0035] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0036] Reference Figures 1-9 The present invention provides a method for enhancing the efficiency of long-distance transport of sea urchins based on dry transport and anhydrous volatile anesthesia, comprising the following steps:

[0037] Anhydrous volatile anesthesia pretreatment: The sea urchin is placed in a sealed container and a natural volatile substance is used as an anesthetic. The volatile substance is loaded and evaporated through an adsorption material to perform anhydrous anesthesia on the sea urchin.

[0038] Inverted fixation to prevent damage: The anesthetized sea urchin is placed upside down on a fixed bracket with a mesh structure in the transport container 1 with the mouth facing upward and the spines facing downward, and the self-locking effect of the spines and the mesh prevents the sea urchin from rolling during transportation.

[0039] Partitioned temperature control and buffering treatment: A functional interlayer is set in the fixed bracket, and temperature control materials and moisturizing materials are placed in the functional interlayer to maintain the transportation environment temperature at 4-15°C and relative humidity>90%;

[0040] Natural Extract Recovery Treatment: After transport, the sea urchins are first placed in seawater containing cactus extract for initial recovery, and then transferred to flowing natural seawater for complete recovery.

[0041] Cactus extract was purchased from Nutri Biotech Services Ltd.

[0042] Furthermore, the adsorption material is cotton balls or filter paper.

[0043] In one embodiment of the present invention, the natural volatile substance is magnolia essential oil, the dosage is 0.01% of the sealed container, and the fumigation time is 30 minutes.

[0044] Magnolia essential oil was purchased from Hubei Kangchun Spice Co., Ltd.

[0045] The volatility of white magnolia essential oil is used to achieve waterless anesthesia. Volatile anesthesia has no chemical residue. Compared with traditional eugenol immersion, the operation time is shortened and the safety is much higher than synthetic anesthetics such as eugenol.

[0046] 0.01% of Magnolia grandiflora essence in a sealed container leaves no residue in tissues after use, and the dosage is only 1 / 10 of that of water-soluble anesthetics, effectively reducing costs.

[0047] Anesthesia steady state is achieved after 30 minutes of fumigation, and the recovery time is shortened to 5 minutes, while traditional immersion recovery takes 15 minutes.

[0048] In one embodiment of the present invention, the mesh diameter of the fixed bracket is smaller than the length of the sea urchin spine and larger than the spine diameter, so that each spine is naturally inserted into the mesh to form a self-locking anti-roll structure, reducing physical damage and effectively reducing the spine breakage rate.

[0049] The mesh diameter is larger than the length of the thorn to prevent it from falling out, and smaller than the diameter of the thorn to facilitate insertion, achieving zero external force fixation.

[0050] In one embodiment of the present invention, the temperature control material is a biological ice pack, preferably four, maintaining 4-15°C.

[0051] In one embodiment of the present invention, the moisturizing material is saturated water-containing sawdust, preferably four pieces, which are packaged with gauze to maintain >90% RH. The gauze-packaged sawdust continuously releases moisture for 72 hours without the need for humidification in the middle.

[0052] In one embodiment of the present invention, the salinity of the seawater containing the cactus extract is 32‰, and the concentration of the cactus extract is 30 ppm.

[0053] In one embodiment of the present invention, the sea urchin is placed in seawater containing cactus extract for initial recovery, wherein the initial recovery soaking time is 0.5 hours.

[0054] In one embodiment of the present invention, the fixing bracket is made of plastic or foam material, which effectively reduces weight.

[0055] In one embodiment of the present invention, the transport container 1 is made of foam material, which has high thermal insulation properties and maintains a low-temperature environment. The volume of the transport container 1 is preferably 35L.

[0056] In one embodiment of the present invention, the fixing bracket comprises:

[0057] A plurality of partition nets 3 with a mesh structure, on which sea urchins are placed, with a functional interlayer formed between two adjacent partition nets 3;

[0058] A plurality of support frames 2 and a plurality of partition nets 3 are alternately arranged.

[0059] The support frame 2 supports the separation nets 3 so that a gap is formed between the two separation nets 3 to form a functional interlayer and prevent the upper and lower layers of sea urchins from contacting each other.

[0060] Experimental comparison:

[0061] Transportation phase (30h):

[0062] Control group: no treatment, simulated transportation for 30 hours.

[0063] Experimental group 1: The rats were anesthetized with 0.01% white magnolia essential oil for 30 minutes before transportation and then transported for 30 hours.

[0064] Experimental group 2: treated in the same way as experimental group 1.

[0065] Initial recovery phase (0.5h):

[0066] Control group & Experimental group 1: Recover in 32% normal seawater for 0.5 h.

[0067] Experimental group 2: recovered in 32% seawater containing 30 ppm of cactus extract for 0.5 h.

[0068] Unified recovery phase (flowing seawater):

[0069] All groups were uniformly transferred to regular flowing seawater, and the sampling time points were: 0h, 3h, 6h, 12h, and 24h after recovery.

[0070] Sampling and testing:

[0071] Sampling time points: 0, 3, 6, 12, 24 h;

[0072] Test content:

[0073] Intestinal and gonadal tissues;

[0074] Enzyme activity indicators: total antioxidant capacity (T-AOC), superoxide dismutase (SOD), glutathione (GSH), and malondialdehyde (MDA).

[0075] like Figure 2-Figure 3 As shown:

[0076] Magnolia essential oil treatment (experimental group 1):

[0077] This treatment significantly (p<0.05) stimulated acute antioxidant responses in the corpuscular and gonadal tissues of purple sea urchins (especially 3 hours after transportation):

[0078] Levels of total antioxidant capacity (T-AOC), superoxide dismutase (SOD), and glutathione (GSH) in intestinal tissue peaked at 3 hours (T-AOC: 5.77 mg / ml, SOD: 46.7 U / ml, GSH: 1017 μmol / L), representing 2-4 times that of the control group. This suggests that essential oil pretreatment may induce stress defense mechanisms, rapidly and significantly improving antioxidant capacity during the initial recovery period (3 hours).

[0079] like Figure 4-Figure 5 As shown:

[0080] Potential delayed damage:

[0081] At 24 h, the malondialdehyde (MDA) content in intestinal tissue increased to 3.90 nmol / mgprot (2.33 nmol / mgprot in the control group), suggesting that lipid peroxidation damage may be delayed in the late stage of stress.

[0082] Cactus extract recovery (experimental group 2):

[0083] Effectively inhibit oxidative damage:

[0084] The MDA content in intestinal tissue was significantly (p<0.05) lower than that in other groups (only 0.83 nmol / mgprot at 24h, 2.33 nmol / mg in the control group, and 3.90 nmol / mg in the experimental group 1).

[0085] like Figure 6-Figure 7 As shown:

[0086] The MDA level in gonadal tissue was only 1.30 nmol / mgprot at 12 h (7.99 nmol / mgprot in experimental group 1), a decrease of 84%, and the effect of inhibiting oxidative damage was extremely significant (p<0.05).

[0087] Maintaining antioxidant homeostasis:

[0088] The gonadal T-AOC at 0h, 12h, and 24h were higher than those in other groups (e.g., 24h: 0.18mg / ml vs. 0.46mg / ml in the control group), indicating that it can more stably maintain the long-term basal antioxidant capacity of the tissue.

[0089] like Figure 8-Figure 9 As shown:

[0090] The fluctuation of GSH levels was relatively gentle (e.g., intestinal GSH at 3h: 436umol / L, significantly lower than 1: 1017umol / L in the experimental group), reflecting a better steady-state regulation ability.

[0091] All experimental groups using the inverted transport structure (i.e., those pretreated with Magnolia grandiflora and / or restored with Cactus truncatum) showed significantly (p<0.05) smaller fluctuations in enzyme activities (such as SOD) and oxidative damage markers (such as MDA) than those in the control group. This strongly demonstrates that the transport device itself can effectively reduce physiological shocks during transport.

[0092] Verification of key indicators: Especially in terms of MDA, a key indicator reflecting lipid peroxidation damage, the low value of experimental group 2 (cactus) was in sharp contrast to the high value of experimental group 1 (magnolia) in the later stage, verifying the synergistic antioxidant protection mechanism of magnolia essential oil (stress initiation) and cactus extract (homeostasis recovery) under the framework of inverted transport structure.

[0093] Actual effectiveness confirmed: Even more notable is the synergistic mechanism combined with the inverted transport structure design: while the survival rate of the group using the traditional transport method (without the patented device) was approximately 80%, the group transported using the patented device achieved a 100% survival rate, with virtually no observed fluid leakage. This result strongly confirms the combined protective efficacy of the inverted transport structure and the Magnolia-Cactus treatment program, both in terms of survival rate and physiological integrity.

[0094] In the description of the present invention, it should be understood that the terms "longitudinal", "transverse", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the accompanying drawings, and are only for the convenience of describing the present invention, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0095] The embodiments described above are merely descriptions of preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Without departing from the spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by persons skilled in the art should fall within the scope of protection defined by the claims of the present invention.

Claims

1. A method for enhancing the efficiency of long-distance transport of sea urchins based on dry transport and anhydrous volatile anesthesia, characterized in that: The following steps are involved: Anhydrous volatile anesthesia pretreatment: The sea urchin is placed in a sealed container and a natural volatile substance is used as an anesthetic. The volatile substance is loaded and evaporated through an adsorption material to perform anhydrous anesthesia on the sea urchin. Inverted fixation and damage prevention: The anesthetized sea urchin is placed upside down on a fixed support with a mesh structure in a transport container (1) with the mouth facing upward and the spines facing downward, and the self-locking effect of the spines and the mesh is used to prevent rolling during transportation; Partitioned temperature control and buffering treatment: a functional interlayer is set in the fixed bracket, and temperature control materials and moisturizing materials are placed in the functional interlayer to maintain the transportation environment temperature at 4-15°C and relative humidity>90%; Natural extract recovery treatment: After transportation, the sea urchins are first placed in seawater containing cactus extract for initial recovery, and then transferred to flowing natural seawater for complete recovery; The natural volatile substance is magnolia essential oil, the dosage is 0.01% of the volume of the closed container, and the fumigation time is 30 minutes.

2. The method for enhancing the synergy of long-distance transport of sea urchins based on dry transport and anhydrous volatile anesthesia according to claim 1, characterized in that: The mesh diameter of the fixing bracket is smaller than the length of the sea urchin spines and larger than the diameter of the spines.

3. The method for enhancing the synergy of long-distance transport of sea urchins based on dry transport and anhydrous volatile anesthesia according to claim 1, characterized in that: The temperature control material is a biological ice bag.

4. The method for enhancing the synergy of long-distance transport of sea urchins based on dry transport and anhydrous volatile anesthesia according to claim 1, characterized in that: The moisturizing material is saturated water-containing sawdust.

5. The method for enhancing the synergy of long-distance transport of sea urchins based on dry transport and anhydrous volatile anesthesia according to claim 1, characterized in that: The salinity of the seawater containing the cactus extract is 32‰, and the concentration of the cactus extract is 30 ppm.

6. The method for enhancing the synergy of long-distance transport of sea urchins based on dry transport and anhydrous volatile anesthesia according to claim 1, characterized in that: The sea urchin is placed in seawater containing cactus extract for initial recovery, wherein the initial recovery soaking time is 0.5 hours.

7. The method for enhancing the synergy of long-distance transport of sea urchins based on dry transport and anhydrous volatile anesthesia according to claim 1, characterized in that: The fixing bracket is made of plastic material or foam material.

8. The method for enhancing the efficiency of long-distance transport of sea urchins based on dry transport and anhydrous volatile anesthesia according to claim 1, characterized in that: The transport container (1) is made of foam material.

9. The method for enhancing the efficiency of long-distance transport of sea urchins based on dry transport and anhydrous volatile anesthesia according to claim 1, characterized in that: The fixing bracket includes: A plurality of partition nets (3) having a mesh structure, wherein the partition nets (3) are used to place sea urchins, and the functional interlayer is formed between two adjacent partition nets (3); A plurality of support frames (2) and a plurality of the partition nets (3) are alternately arranged.

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