External traditional Chinese medicine preparation capable of relieving acute soft tissue injury and promoting tissue regeneration

Abstract

The invention discloses an external traditional Chinese medicine preparation capable of relieving acute soft tissue injury and promoting tissue regeneration, which is a traditional Chinese medicine extracting solution and is prepared from the following raw materials in parts by mass: 20 parts of flos carthami, 20 parts of angelica sinensis, 10 parts of frankincense, 10 parts of myrrh, 15 parts of radix aconiti, 15 parts of radix aconiti agrestis, 20 parts of garden balsam stem, 10 parts of ligusticum wallichii, 15 parts of rheum officinale, 20 parts of radix angelicae and 10 parts of dragon's blood. The traditional Chinese medicine composition is safe to use and small in side effect, can relieve the inflammation level, reduce oxidative stress in injured tissues, promote remodeling and regeneration of skeletal muscles, effectively improve the symptoms of soft tissue injury and improve the pathological state caused by injury and has the effects of promoting blood circulation to remove blood stasis, dredging channels and collaterals and relieving swelling and pain due to the synergistic effect of the medicines.

Description

Technical Field

[0001] The present invention relates to a traditional Chinese medicine preparation, in particular to an external-use traditional Chinese medicine preparation capable of relieving acute soft tissue damage and promoting tissue regeneration. Background Art

[0002] Acute soft tissue injury is a common clinical trauma, primarily involving damage to muscles, ligaments, tendons, and other tissues. Its pathological features typically include local inflammation, cell necrosis, tissue edema, and functional impairment. In recent years, the pathogenesis of soft tissue injury has been gradually revealed, with inflammation and oxidative stress considered to be key pathological processes.

[0003] After acute soft tissue injury, local tissue releases a variety of inflammatory factors, such as interleukins (IL-6, IL-1β), tumor necrosis factor α (TNF-α), and prostaglandin E2 (PGE2). These factors regulate inflammatory cell infiltration and tissue repair by activating related signaling pathways. However, excessive or persistent inflammatory responses may lead to tissue fibrosis, delayed repair, and even dysfunction. Furthermore, increased production of reactive oxygen species (ROS) in local tissues exceeds the body's antioxidant capacity, leading to lipid peroxidation, protein oxidation, and DNA damage, further exacerbating the inflammatory response and tissue damage. Furthermore, malondialdehyde (MDA) levels in damaged tissues are significantly elevated, while superoxide dismutase (SOD) activity is decreased, indicating the importance of oxidative stress in soft tissue injury. Therefore, regulating the expression of inflammatory factors and the activation of related signaling pathways, scavenging ROS, or enhancing antioxidant capacity may provide new strategies for the treatment of soft tissue injury.

[0004] Currently, the primary treatment for acute soft tissue injuries is oral nonsteroidal anti-inflammatory drugs (NSAIDs). While these drugs can effectively relieve pain and inflammation, long-term use can cause side effects such as gastrointestinal damage and liver and kidney impairment. Topical medications, such as diclofenac diethylamine emulsion (trade name: Voltaren), are commonly used to relieve pain caused by muscle and soft tissue sprains, strains, contusions, strains, back injuries, and joint pain. However, long-term, large-area use can easily lead to adverse reactions such as general rashes and allergic reactions. There are also topical Chinese herbal preparations (liniments, tinctures, etc.) for the relief of acute soft tissue injuries, such as Yunnan Baiyao tincture, Zhenggushui, and safflower oil. While these are safe and have minimal side effects, they are often used for mild soft tissue contusions and sprains. Therefore, the development of new topical medications with anti-inflammatory, antioxidant, and tissue repair-promoting properties has become a research priority. Summary of the Invention

[0005] The present invention aims to solve the above-mentioned technical problems existing in the prior art and provides an external Chinese medicine preparation that can relieve acute soft tissue damage and promote tissue regeneration.

[0006] The technical solution of the present invention is: an external Chinese medicine preparation that can relieve acute soft tissue injury and promote tissue regeneration, which is a Chinese medicine extract. The raw materials used and the quality ratio are as follows: 20 grams of safflower, 20 grams of angelica, 10 grams of frankincense, 10 grams of myrrh, 15 grams of Chuanwu, 15 grams of Kusnezoffii, 20 grams of radix polygoni lobatae, 10 grams of Chuanxiong, 15 grams of rhubarb, 20 grams of angelica dahurica, and 10 grams of dragon's blood.

[0007] The preferred Chinese herbal medicine extract is a tincture obtained by soaking the Chinese herbal medicine in 75% alcohol.

[0008] The present invention provides an external Chinese medicine preparation that can relieve acute soft tissue injury and promote tissue regeneration. The preparation is safe to use and has few side effects. The drugs work synergistically to reduce inflammation levels, reduce oxidative stress in damaged tissues, promote skeletal muscle remodeling and regeneration, effectively improve symptoms of soft tissue injury, and improve pathological conditions caused by the injury. The preparation has the effects of promoting blood circulation, removing blood stasis, unblocking meridians, and reducing swelling and relieving pain. BRIEF DESCRIPTION OF THE DRAWINGS

[0009] Figure 1 This is a schematic diagram of an embodiment of the present invention for changing the pathological state of injured muscles in rats with soft tissue injury.

[0010] Figure 2 Schematic diagram of reducing inflammation levels in damaged tissues according to an embodiment of the present invention.

[0011] Figure 3 Schematic diagram of reducing oxidative stress in damaged tissue according to an embodiment of the present invention.

[0012] Figure 4 This is a schematic diagram of reducing the Myostatin content in damaged tissues according to an embodiment of the present invention. DETAILED DESCRIPTION

[0013] The external Chinese medicinal preparation of the present invention can relieve acute soft tissue injury and promote tissue regeneration. The raw materials and their weights are as follows: 20g of safflower, 20g of angelica, 10g of frankincense, 10g of myrrh, 15g of Chuanwu, 15g of Kusnezoffii, 20g of peony root, 10g of Chuanxiong, 15g of rhubarb, 20g of angelica dahurica, and 10g of Sanguisorba officinalis. The above 11 medicinal materials are crushed and mixed, and then soaked in 825ml of 75% alcohol for 7 days. The mixture is filtered and the supernatant is collected.

[0014] experiment:

[0015] One hundred and twenty SD rats were randomly divided into five groups (n = 24, "n" represents the number of animals in each group): a blank group (Control), a soft tissue injury model group (Model), a Voltaren-treated group (Voltaren), a low-dose group (RTL-L), and a high-dose group (RTL-H). A soft tissue injury model was established in SD rats using conventional methods. The procedure was as follows: 2% sodium pentobarbital solution (40 mg / kg) was injected intraperitoneally to induce anesthesia. After anesthesia, the hair on the right thigh was shaved with a shaver, followed by application of animal depilatory cream to further remove any remaining hair, ensuring that the area was clean. The rats were placed supine on a homemade contusion table. To prevent fractures, a soft sponge pad was placed under the hind limbs. Once ready, a 500 g weight was dropped vertically from a height of 20 cm onto the rectus muscle of the thigh. This procedure was repeated six times to successfully establish a closed soft tissue injury model. After the model was established, it was clearly observed that the injured hind limb muscles of the rats were swollen. The swelling rate increased 2 hours after the injury and remained for a long time thereafter without any significant reduction. The control group received no treatment; the model group received no treatment after the model was established; the Voltaren group received Voltaren topical treatment after the model was established; and the RTL-L group received 4g·kg -1 The tincture of the present invention was applied externally for treatment; after the RTL-H group model was established, 8g·kg -1 The tincture of the present invention was administered for external application. Rats were sacrificed in batches on the first, fourth, and seventh days after administration, and muscle tissue was collected and observed for pathological changes on the first, fourth, and seventh days of treatment. Myostatin content in damaged muscle tissue of the rats was determined by immunohistochemistry; IL-6, IL-1β, PGE2, and TNF-α content in damaged tissue was determined by ELISA; and MDA and SOD oxidative stress factor content in damaged muscle tissue of the rats was determined.

[0016] 2. Experimental Results 2.1 Improve the pathological state of soft tissue injury HE staining was used to observe the pathological state of damaged muscle tissue in each group. Figure 1As shown. The results showed that under normal conditions, the muscle cells in the muscle tissue of the rats in the Control group were tightly and regularly arranged, the nuclei were regularly distributed at the edges of the cells, and the cytoplasmic spaces were also relatively uniform. The cells in the muscle tissue of the rats in the Model group were of different sizes, the spaces were wider, the cytoplasm was unevenly stained, and muscle cells ruptured; on the 4th day, cells were seen floating free in the intercellular matrix, and the local cytoplasm became darker in color; on the 7th day, the cells in the groups gradually became denser and fused in the region. On the first day, the muscle cells in the Voltaren, RTL-L, and RTL-H groups were loosely arranged and the spaces were enlarged; on the 4th day, the inflammatory infiltration in the groups began to ease; on the 7th day, the muscle cells were more regularly arranged than before and were tightly fused. The cell morphology of each treatment group was significantly better than that of the Model group, among which the treatment effect of the RTL-H group was the most significant.

[0017] 2.2 Improve inflammation levels in damaged tissues The ELISA method was used to detect the contents of IL-6, IL-1β, PGE2, and TNF-α in rat muscle tissue. Figure 2 The results showed that IL-6 in rat muscle tissue ( Figure 2 A), IL-1β ( Figure 2 B), PGE2 ( Figure 2 C), TNF-α ( Figure 2 At the same time point, the content of D) in the Model group increased compared with the Control group, which was statistically significant ( P <0.01); compared with the Model group, the levels of IL-6, IL-1β, PGE2, and TNF-α in the injured muscle tissues of the rats in the Voltaren group, RTL-L group, and RTL-H group were reduced to varying degrees, and the therapeutic effect of the RTL-H group was the most significant.

[0018] RT-qPCR was used to detect TNF-α in the injured tissues of rats. Figure 2 E), IL-6 ( Figure 2 F), IL-1β ( Figure 2 G) mRNA content, the Model group increased compared with the Control group, with statistical significance ( P <0.01). At the same time point, the Voltaren group, RTL-L group, and RTL-H group all showed a decrease compared with the Model group, and the decrease was statistically significant ( P <0.05). This indicates that after drug intervention, the mRNA levels of TNF-α, IL-6, and IL-1β in the injured muscle tissue of rats decreased, and the therapeutic effect of the RTL-H group was the most significant.

[0019] 2.3 Improve oxidative stress levels in damaged tissues The oxidative stress levels in damaged tissues were determined using SOD and MDA assay kits. Figure 3 The results showed that within different time points, the SOD activity value in the damaged tissue of the same group showed a gradual increase from the first day to the seventh day, and the MDA activity showed a trend of first increasing and then decreasing from the first day to the seventh day, and the difference was statistically significant ( P <0.01)( Figure 3 A). At the same time point, compared with the Control group, the SOD content in the Model group decreased and the MDA content increased, and the difference was statistically significant ( P <0.01); Compared with the Model group, the SOD content of the Voltaren group, RTL-L group, and RTL-H group increased, and the MDA content decreased. Among them, the treatment effect of the RTL-H group was the most significant, and the difference was statistically significant ( P <0.01)( Figure 3 B).

[0020] 2.4 Schematic diagram of reducing myostatin content in damaged tissues Immunohistochemistry was used to determine the content of Myostatin in the damaged tissue. Figure 4 The results showed that the intra-group comparison showed that the average optical density of Myostatin in rats in each group was higher on the 4th day, and the overall trend was a rapid increase followed by a slow decline, which was statistically significant ( P <0.05, P <0.01). At the same time point, inter-group comparison: on the 4th and 7th days, the expression values ​​of Myostatin in the Voltaren group, RTL-L group, and RTL-H group were all lower than those in the Model group, and the differences were statistically significant ( P <0.01); This suggests that the Voltaren, RTL-L, and RTL-H groups can reduce the expression of Myostatin in damaged tissues and counteract the inhibitory effects of elevated Myostatin on the proliferation and differentiation of muscle satellite cells, with the RTL-H group showing the most significant effect ( Figure 4A, B). Myostatin is a key negative regulator of muscle growth, exerting its effects by binding to serine / threonine kinase receptors (ActRⅡB and ALK4 / 5) on the cell membrane surface. In the cell nucleus, it binds to specific DNA sequences and regulates the transcription of a series of target genes, which are primarily associated with processes such as muscle cell proliferation, differentiation, and protein synthesis. Through this signal transduction pathway, myostatin inhibits the proliferation and differentiation of muscle satellite cells, thereby limiting muscle growth. In this experiment, the average optical density of myostatin showed a rapid increase followed by a slow decline. On days 4 and 7, the average optical density of myostatin in each treatment group was lower than that in the model group, indicating that RTL can promote skeletal muscle remodeling and regeneration by improving the morphology and arrangement of skeletal muscle cells and downregulating myostatin expression.

Claims

1. A Chinese medicine preparation for external use that can relieve acute soft tissue damage and promote tissue regeneration, which is a Chinese medicine extract, characterized in that The raw materials used and their quality are as follows: 20g of safflower, 20g of angelica, 10g of frankincense, 10g of myrrh, 15g of Chuanwu, 15g of Kusnezoffii, 20g of Radix Polygoni Multiflori, 10g of Chuanxiong, 15g of rhubarb, 20g of Angelica dahurica, and 10g of Sanguisorba officinalis.

2. The external-use Chinese medicinal preparation capable of relieving acute soft tissue injury and promoting tissue regeneration according to claim 1, characterized in that Chinese herbal medicine extract is a tincture obtained by soaking Chinese herbal medicine in 75% alcohol.

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