Pollen composition, fermentation product as well as preparation method and application of pollen composition

Abstract

The invention relates to the technical field of pollen fermentation, in particular to a pollen composition, a fermentation product as well as a preparation method and application thereof. The pollen composition comprises pine pollen, sunflower pollen, buckwheat pollen, corn pollen and rape pollen, and the mass ratio of the pine pollen to the sunflower pollen to the buckwheat pollen to the corn pollen to the rape pollen is (2-5): (1.5-2): (1-2): (1-2): (1-2). According to the invention, the proportion of the pollen is adjusted within a specific range, so that the process parameters are optimized, and the effects of resisting oxidation, resisting inflammation, resisting aging and improving immunity can be synchronously improved.

Description

Technical Field

[0001] The present invention relates to the technical field of pollen fermentation, and in particular to a pollen composition, a fermentation product, and a preparation method and application thereof. Background Art

[0002] Pollen is the genetic material of plants, containing the reproductive nucleus and nutrients for reproduction. It is a highly concentrated natural nutrient reservoir. Pollen contains a rich array of nutrients, including protein, carbohydrates, unsaturated fatty acids, phenols, vitamins, and trace elements. These nutrients can boost the body's immune system and maintain the youthfulness of organs, thereby delaying aging.

[0003] Some characteristics of pollen itself, such as thick walls, miscellaneous bacteria, special smell and allergenicity, make it unsuitable for direct consumption. Microbial fermentation can break the walls, sterilize, desensitize and increase nutrients, thus turning pollen into a nutritious, safe and reliable advanced nutritional product. Fermentation is the best way to break the pollen walls and release nutrients. At the same time, the fermentation process also promotes the improvement of nutrients due to the participation of some special bacteria. At present, pollen fermentation usually chooses probiotics (lactic acid bacteria, yeast, etc.) screened from bee food or edible fungi. The use of fungal fermentation has very outstanding advantages. In addition to breaking the pollen walls and releasing the nutrients of the pollen itself, different fungi can also produce different active substances, which can be fermented in two directions with the pollen, greatly increasing the content of active ingredients in the fermentation liquid, and obtaining health benefits that were previously absent or weaker with pollen.

[0004] Ganoderma lucidum is a fungus. Modern research shows that it has the functions of inhibiting tumors, enhancing immunity, delaying aging, and protecting the liver. It has become an important resource in the research and development of health foods and traditional Chinese medicine products.

[0005] Chinese patent CN110755344A discloses a Ganoderma lucidum-Polygonatum bidirectional fermentation process. Ganoderma lucidum and Polygonatum sibiricum are fermented through bidirectional fermentation technology. It was found that the ability of the Ganoderma lucidum and Polygonatum bidirectional fermentation liquid to remove DPPH is significantly better than that of the Polygonatum sibiricum original liquid. It can be used as a potential cosmetic anti-aging raw material. The Ganoderma lucidum and Polygonatum bidirectional fermentation liquid has a significant effect on inhibiting hyaluronidase and can be developed and used as a potential cosmetic raw material for anti-inflammatory or acne treatment. The application of the Ganoderma lucidum and Polygonatum fermentation liquid in facial masks has the effects of moisturizing, hydrating and whitening.

[0006] The study in the literature "Optimization of the fermentation process of composite pollen Ganoderma lucidum and analysis of active substance components" showed that when Ganoderma lucidum and composite pollen were fermented together, the fermentation process conditions were pollen addition of 1.5%, initial pH of 7, and fermentation time of 11 days. The polysaccharide content of the composite pollen Ganoderma lucidum fermentation liquid was (393.69±4.09) mg / 100 mL, the total flavonoid content was (5.63±0.20) mg / 100 mL, and the iron ion reduction antioxidant capacity reached (2 .303±0.143 μmol / mL; the polysaccharides in the fermentation broth consisted of 10 monosaccharides: mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose, and fucose, with glucose (49.42%) being the highest. The fermentation broth also contained five phenolic acids: quercetin, p-hydroxybenzoic acid, luteolin, apinic acid, and kaempferol, with kaempferol (683.71 mg / kg) being the highest. However, the other efficacy of the product obtained in this study was not significant.

[0007] Therefore, it is of great significance to utilize fermentation technology to obtain fermentation products with significantly improved multiple functions. Summary of the Invention

[0008] In view of this, the present invention aims to provide a pollen composition, fermentation product, preparation method, and application thereof. During research, the present invention discovered that during the bidirectional fermentation of Ganoderma lucidum and pollen, the pollen ratio has a significant impact on the efficacy of the final product. The present invention unexpectedly discovered that adjusting the pollen ratio within a specific range can achieve simultaneous improvements in antioxidant, anti-inflammatory, anti-aging, and immunity-enhancing effects, thereby producing a functional food with better overall effects and improving social health.

[0009] In order to achieve the above-mentioned purpose of the invention, the technical solution of the present invention is as follows:

[0010] On the one hand, the present invention provides a pollen composition comprising pine pollen, sunflower pollen, buckwheat pollen, corn pollen and rapeseed pollen, wherein the mass ratio of pine pollen, sunflower pollen, buckwheat pollen, corn pollen and rapeseed pollen is 2-5:1.5-2:1-2:1-2:1-2.

[0011] Preferably, the mass ratio of the pine pollen, sunflower pollen, buckwheat pollen, corn pollen and rapeseed pollen is 2-3:2:2:1.5-2:1.5-2.

[0012] Further preferably, the mass ratio of the pine pollen, sunflower pollen, buckwheat pollen, corn pollen and rapeseed pollen is 3:2:2:1.5:1.5.

[0013] In another aspect, the present invention provides a fermentation product obtained by fermenting the above-mentioned pollen composition.

[0014] Preferably, the fermentation product is obtained by fermenting the above-mentioned pollen composition with Ganoderma lucidum.

[0015] Preferably, the fermentation product is the supernatant obtained by fermenting the above-mentioned pollen composition with Ganoderma lucidum.

[0016] Preferably, the fermentation comprises the steps of:

[0017] Under aseptic operation, the seed culture liquid of Ganoderma lucidum is taken, added into the fermentation medium containing pollen, mixed evenly, cultured, separated, and the supernatant is taken as the fermentation liquid.

[0018] Preferably, the fermentation medium for the pollen comprises acacia honey, KH2PO4 and MgSO4·7H2O.

[0019] Further preferably, each 200 mL comprises 1-20 g of acacia honey, 0.1-1 g of KH2PO4, and 0.05-0.5 g of MgSO4·7H2O. Even more preferably, each 200 mL comprises 10 g of acacia honey, 0.6 g of KH2PO4, and 0.3 g of MgSO4·7H2O.

[0020] Further preferably, the pollen-containing fermentation medium is prepared according to the following steps: taking acacia honey, KH2PO4 and MgSO4·7H2O, and making up to volume with water.

[0021] Preferably, the pollen content in the pollen-containing fermentation medium is 1-100 g / L, more preferably 5-50 g / L, and most preferably 40 g / L.

[0022] Preferably, the separation method is centrifugation. More preferably, the centrifugation condition is: 8000-10000 r / min for 15-20 min.

[0023] Preferably, after uniform mixing, the step of adjusting the pH is further included. More preferably, the pH is adjusted to 3-7, and most preferably, the pH is adjusted to 5.

[0024] Preferably, the method for preparing the seed culture solution of the Ganoderma lucidum species comprises the following steps:

[0025] Under aseptic operation, the activated bacterial strain is inoculated into the basic liquid culture medium, cultured, and a seed culture solution is obtained.

[0026] Further preferably, the culture conditions are: 22-28° C., 80-250 r / min shaking culture for 5-15 days; most preferably: 25° C., 120-180 r / min shaking culture for 5-10 days.

[0027] Further preferably, the steps of preparing the activated bacterial strain include:

[0028] Under sterile operation, the Ganoderma lucidum strains were inoculated into PDA plate culture medium, activated, and cultured until the mycelium covered the plate. Furthermore, the culture temperature was 22-28°C, and most preferably 25°C.

[0029] In another aspect, the present invention provides a baking powder obtained by drying the above-mentioned fermentation product.

[0030] Preferably, the drying is any one of spray drying, oven drying, freeze drying, reduced pressure drying, and boiling drying, more preferably spray drying.

[0031] More preferably, the spray drying conditions are: maltodextrin addition amount is 2-10%, inlet air temperature is 120-180° C., and flow rate is 700-1500 mL / h.

[0032] In another aspect, the present invention provides an application of the pollen composition, fermentation product, and fermentation powder in the preparation of foods, health products, or medicines with antioxidant effects.

[0033] In another aspect, the present invention provides an application of the pollen composition, fermentation product, and fermentation powder in the preparation of food, health care product, or medicine with anti-inflammatory effects.

[0034] In another aspect, the present invention provides the use of the pollen composition, fermentation product, and fermentation powder in the preparation of foods, health products, or medicines with anti-aging effects.

[0035] In another aspect, the present invention provides an application of the pollen composition, fermentation product, and fermentation powder in the preparation of foods, health products, or medicines for improving immunity.

[0036] When preparing food or health products, you may also add excipients that are acceptable to food, including but not limited to:

[0037] 1) Edible flavors: The use of edible flavors in solid and liquid preparations should comply with the provisions of GB30616, and their components should be included in the "National Food Safety Standard - Standard for the Use of Food Additives" (GB2760) or Appendix A "List of Excipients Allowed for Use in Food Flavors" in GB30616.

[0038] 2) Coating premix and film-forming agent: Since the production process requires them as excipients for coating, anti-adhesion and other purposes that cannot be quantified, their components should be included in the "National Food Safety Standard for the Use of Food Additives"

[0039] (GB2760) or the current Pharmacopoeia of the People's Republic of China, the dosage can be used in appropriate amounts according to production needs.

[0040] 3) Oligosaccharide raw materials: such as FOS (fructooligosaccharides), GOS (galacto-oligosaccharides), and IMO (xylooligosaccharides), often referred to as prebiotics, have the effect of improving intestinal health.

[0041] 4) DHA and ARA: DHA (docosahexaenoic acid) and ARA (arachidonic acid) are common functional ingredients in infant food.

[0042] 5) Probiotics: such as Bb-12 (Bifidobacterium lactis), can improve intestinal function.

[0043] When preparing medicines, pharmaceutically acceptable excipients may also be added.

[0044] The drug can be prepared into dosage forms such as pills, capsules, granules, oral liquids, powders, tablets, lozenges, and lozenges. Suitable drug carriers in the art can be selected for different dosage forms.

[0045] The pharmaceutical carrier used can be a solid, liquid or gas. Examples of solid carriers include lactose, kaolin, sucrose, talc, gelatin, agar, pectin, gum arabic, magnesium stearate and stearic acid. Examples of liquid carriers include syrup, peanut oil, olive oil and water. Examples of gaseous carriers include carbon dioxide and nitrogen.

[0046] When preparing compositions for oral dosage forms, any convenient pharmaceutical medium can be used. For example, water, ethanol, oils, alcohols, flavoring agents, preservatives, colorants, and the like can be used to form oral liquid formulations, such as suspensions and solutions; while carriers, such as starches, sugars, microcrystalline cellulose, diluents, granulating agents, emulsifiers, lubricants, binders, and disintegrants can be used to form oral solid formulations, such as powders, capsules, and tablets. Tablets and capsules are preferred oral dosage units using solid pharmaceutical carriers due to their ease of administration. Tablets can be coated using standard aqueous or nonaqueous techniques.

[0047] Tablets containing the Chinese medicine composition of the present invention can be prepared by tableting or molding, and one or more auxiliary ingredients or adjuvants can be used. The active ingredient can be compressed into a free-flowing form (such as powder or granules) in a suitable machine, and can be prepared by mixing with a binder, lubricant, inert diluent, surfactant or dispersant. Molded tablets can be molded in a suitable machine, i.e., a powdered mixture moistened with an inert liquid diluent, preferably containing about 0.05 mg to about 5 g of active ingredient per tablet, and preferably containing about 0.05 mg to about 5 g of active ingredient per sachet or capsule. For example, a preparation intended for oral administration to the human body may contain about 0.5 mg to about 5 g of active drug, mixed with an appropriate and convenient carrier material, which may account for about 5% to 95% of the total composition. Unit dosage forms typically contain about 1 mg to about 2 g of active ingredient, typically 25 mg, 50 mg, 100 mg, 200 mg, 300 mg, 400 mg, 500 mg, 600 mg, 800 mg or 1000 mg.

[0048] Pharmaceutical compositions suitable for parenteral administration of the present invention can be prepared as aqueous solutions or suspensions of the active compound and can include suitable surfactants, such as hydroxypropylcellulose. Dispersions can also be prepared in glycerol, liquid polyethylene glycol, and oil mixtures thereof. In addition, preservatives can be added to prevent the harmful growth of microorganisms.

[0049] Medicine of the present invention can be the form that is suitable for topical use, for example aerosol, cream, ointment, lotion, powder or the like.In addition, composition can be suitable form and be used for transdermal administration device.Can use Chinese medicine composition of the present invention, prepare these prescriptions by conventional processing method.For example, by mixing hydrophilic material and water, and about 5wt% to about 10wt% compound, prepare cream or ointment with required consistency.

[0050] The medicament of the present invention can be in a form suitable for rectal administration, wherein the carrier is a solid. It is best to prepare the mixture into unit dose suppositories. Suitable carriers include cocoa butter and other materials commonly used in the art. Suppositories can be prepared by first forming a composition containing a softened or melted carrier, followed by cooling and shaping in a mold.

[0051] In addition to the above-mentioned carrier components, the above-mentioned pharmaceutical preparations may include (if applicable) one or more additional carrier components, such as diluents, buffers, flavoring agents, binders, surfactants, thickeners, lubricants, preservatives (including antioxidants), etc. In addition, other excipients, such as lactose, starch, cellulose derivatives, magnesium stearate, stearic acid, colorants and flavoring agents, etc., may be added, and the components containing the Chinese medicine composition of the present invention may also be prepared in the form of powder or concentrate.

[0052] The beneficial effects of the present invention are:

[0053] The present invention optimizes process parameters by adjusting the proportion of pollen within a specific range, which can achieve simultaneous improvement of antioxidant efficacy, anti-inflammatory efficacy, anti-aging efficacy, and immunity-enhancing efficacy, thereby obtaining functional foods with better comprehensive effects and improving social health levels. BRIEF DESCRIPTION OF THE DRAWINGS

[0054] Figure 1 This is the experimental result of the anti-wrinkle ability of fermentation products.

[0055] In the figure, # represents a significant difference compared with the blank group, p < 0.05; * represents a significant difference compared with the model group, p < 0.05; ** represents a very significant difference compared with the model group, p < 0.01.

[0056] Figure 2 This is a graph showing the results of immune changes detection.

[0057] In the figure, # represents a significant difference compared with the blank group, p < 0.05; * represents a significant difference compared with the model group, p < 0.05.

[0058] Figure 3 This is the antioxidant capacity test result diagram.

[0059] In the figure, # represents a significant difference compared with the blank group, p < 0.05; * represents a significant difference compared with the model group, p < 0.05; ** represents a very significant difference compared with the model group, p < 0.01.

[0060] Figure 4 This is the result of anti-aging protein expression detection.

[0061] In the figure, ## represents a very significant difference compared with the blank group, p < 0.01; * represents a significant difference compared with the model group, p < 0.05; ** represents a very significant difference compared with the model group, p < 0.01.

[0062] Figure 5 This figure shows the test results on the lifespan of nematodes.

[0063] Figure 6 This is a graph showing the experimental results on the anti-inflammatory ability of fermentation products.

[0064] In the figure, ## represents a very significant difference compared with the blank group, p < 0.01; * represents a significant difference compared with the model group, p < 0.05; ** represents a very significant difference compared with the model group, p < 0.01. DETAILED DESCRIPTION

[0065] The following non-limiting examples are provided to enable those skilled in the art to more fully understand the present invention, but are not intended to limit the present invention in any way. The following are merely illustrative of the scope of the present invention, and those skilled in the art may make various changes and modifications to the present invention based on the disclosed content.

[0066] Examples 1-4

[0067] Pine pollen, sunflower pollen, buckwheat pollen, corn pollen, and rapeseed pollen were selected, with pine pollen as the main ingredient for the design of the blend ratio, as shown in the following table:

[0068]

[0069] Examples 1-4 were prepared according to the following method:

[0070] (1) Bacteria activation

[0071] Store in PDA slant medium and place in a refrigerator at 4°C. Aseptically inoculate Ganoderma lucidum in the center of a PDA plate, activate, and culture at 25°C until the mycelium covers the entire plate.

[0072] (2) Liquid culture: Under sterile operation, take bacterial blocks and inoculate them into Erlenmeyer flasks containing basic liquid culture medium. Culture them in a shaking incubator at 25°C and 150 rpm for 7 days to obtain seed liquid.

[0073] (3) Pollen fermentation

[0074] Aseptically add the seed culture medium to a fermentation medium containing composite pollen (40 g / L of pollen), mix thoroughly, adjust the pH to 5, culture in a shake flask, and centrifuge at 8,000–10,000 rpm for 15–20 minutes. The supernatant is the fermentation broth. The fermentation medium is prepared as follows: 10 g of acacia honey, 0.6 g of KH2PO4, and 0.3 g of MgSO4·7H2O, and dilute to 200 mL with distilled water.

[0075] (4) Spray drying

[0076] Take the fermentation liquid after centrifugation, add maltodextrin according to the mass of the fermentation liquid, stir evenly, and use a spray dryer to dry. The spray drying conditions are: maltodextrin addition amount is 2-10%, inlet air temperature is 120-180℃, and flow rate is 700-1500mL / h.

[0077] Example 5

[0078] The difference from Example 3 is that in step (3), the pH is adjusted to 3. The rest are the same.

[0079] Example 6

[0080] The difference from Example 3 is that in step (3), the pH is adjusted to 7. The rest are the same.

[0081] Comparative Example 1

[0082] The difference from Example 3 is that in step (3), the pH is adjusted to 7.5. The rest are the same.

[0083] Comparative Example 2

[0084] The difference from Example 3 is that the composite pollen formula is: pine pollen (30%) + sunflower pollen (30%) + buckwheat pollen (20%) + corn pollen (10%) + rapeseed pollen (10%). The rest are the same.

[0085] Test Example 1

[0086] The total polysaccharide and total flavonoid contents were tested, and the antioxidant capacity was evaluated. A comprehensive score was performed using the maximum normalization method to determine the optimal combination. The results are shown in the table below:

[0087]

[0088] The results showed that the optimal combination was Example 3 (pine pollen (30%) + sunflower pollen (20%) + buckwheat pollen (20%) + corn pollen (15%) + rapeseed pollen (15%)).

[0089] Test Example 2: Analysis of ingredients in Ganoderma lucidum pollen before and after bidirectional fermentation

[0090] The content of all pollen active ingredients in Examples 1-4 was tested, and the content of active ingredients in the composite pollen substrate was calculated according to Example 3. After fermentation, the content of active ingredients in the fermented product was tested and compared. The results are shown in the following table:

[0091]

[0092]

[0093] The results show that after dual fermentation of Ganoderma lucidum and various pollen types, the polysaccharide and saponin contents increased significantly, by 8.4 times and 2.1 times, respectively. This indicates that Ganoderma lucidum and pollen can promote the production of polysaccharides and saponins in a dual manner, which is related to the composition characteristics of Ganoderma lucidum. The polyphenol content also increased by 4.4 times.

[0094] Test Example 3: Anti-wrinkle ability test of fermentation products

[0095] Sample selection Example 3, Comparative Example 1, Comparative Example 2, using HFF-1 human fibroblasts, to establish a photoaging model, and to investigate the content of elastin, glycosaminoglycans, type I / III collagen, and transforming growth factor TGF-β in the cells after the action of baking powder. The index detection was performed using an enzyme-linked immunosorbent assay kit (ELISA). The results are shown in Figure 1 .

[0096] Test Example 4: Safety Verification of Fermentation Products

[0097] Sample Selection Example 3.

[0098] Sixty experimental mice were randomly divided into 6 groups, with 10 mice in each group, half of them were male and half were female.

[0099] Blank group: subcutaneous injection of normal saline + 0.9NaCl solution and gavage.

[0100] Model group: subcutaneous injection of 120 mg / kg D-galactose + 0.9 NaCl solution and oral gavage.

[0101] Control group: subcutaneous injection of 120 mg / kg D-galactose + Vc gavage.

[0102] Low-dose group: subcutaneous injection of 120 mg / kg D-galactose + oral gavage of low-dose product.

[0103] Medium-dose group: subcutaneous injection of 120 mg / kg D-galactose + oral gavage of medium-dose product.

[0104] High-dose group: subcutaneous injection of 120 mg / kg D-galactose + oral gavage of high-dose product.

[0105] The mice were reared for 30 days, and their behavior, coat color, weight, and growth were observed and recorded daily to determine the safety impact of the fermentation products.

[0106] The results are shown in the table below:

[0107]

[0108]

[0109] The results show that the product prepared by the present invention is safe.

[0110] Test Example 5: Mouse Immunity Enhancement Experiment (Immune Organ Index)

[0111] The samples selected were Example 3, Comparative Example 1, and Comparative Example 2, and the animal experiment scheme was the same as that of Test Example 4.

[0112] At the end of the experiment, mice in each group were fasted for 12 h, weighed, and slaughtered. The spleen was separated and weighed, and the immune organ index was calculated. Figure 2 .

[0113]

[0114] Test Example 6: Antioxidant Capacity Test

[0115] The animal experiment protocol was the same as in Example 5.

[0116] Blood was collected from the mouse orbital cavity and serum GSH-Px and MDA levels were measured using a kit. Liver tissue was removed and a 10% tissue homogenate was prepared by adding normal saline at a mass-to-volume ratio of 1:9 (mL). The homogenate was centrifuged at 4°C and 3000 rpm for 10 minutes, and the supernatant was collected for analysis.

[0117] The GSH-Px and total superoxide dismutase (SOD) levels of mouse liver homogenate samples were collected using a kit. Figure 3 .

[0118] Test Example 7: Anti-aging protein expression experiment

[0119] The samples selected were Example 3, Comparative Example 1, and Comparative Example 2, and the animal experiment scheme was the same as that of Test Example 4.

[0120] Liver tissue protein was extracted using RIPA lysis buffer, and 80 μg of protein sample was loaded for electrophoresis and transfer. The membrane was incubated with SIRT6 antibody (abcam) at a ratio of 1:1000 and β-actin antibody at a ratio of 1:800 at 4°C overnight. Antibody II (1:2500) was hybridized with the membrane at room temperature for 1 hour. ECL was used for color development and images were captured. A grayscale scanner was used to analyze the grayscale ratio of SIRT6 and the corresponding β-actin / erk bands to indicate their relative expression levels. The results are shown in Figure 2. Figure 4 .

[0121] Test Example 8: Effect of Ganoderma Lucidum Pollen Fermentation Powder on Nematode Lifespan

[0122] Appropriate amounts of samples from Example 3, Comparative Example 1, and Comparative Example 2 were weighed, dissolved in purified water, sterilized by a membrane, and mixed with the OP50 bacterial solution to obtain a fermentation powder bacterial solution.

[0123] Nematode grouping:

[0124] Blank group: 30 nematodes, cultured on NGM medium coated with OP50 bacterial solution;

[0125] Sample group: 30 nematodes, cultured on NGM medium coated with yeast powder solution.

[0126] The survival status of nematodes was tracked and observed, the number of nematode deaths was recorded, and statistical analysis and drawing analysis were performed using Prism software.

[0127] See the results Figure 5 .

[0128] Test Example 9: Anti-inflammatory ability test of fermentation products

[0129] The samples selected were Example 3, Comparative Example 1, and Comparative Example 2.

[0130] RAW264.7 cells were used to establish a validation model using LPS (lipopolysaccharide) to investigate the changes in NO content in cells after the action of yeast powder. The indicator was detected using a nitric oxide kit (Biyuntian Biotechnology). Figure 6 .

[0131] Figure 1-6 The results show that the anti-wrinkle ability, immunity-enhancing ability, antioxidant ability, anti-aging ability, nematode lifespan-enhancing ability and anti-inflammatory ability of Example 3 are significantly better than those of Comparative Examples 1 and 2, indicating that a specific formula ratio and a specific pH environment have a significant effect on the activity of the fermentation product, and that, at a specific ratio and pH, a fermentation product with significantly improved comprehensive effects can be obtained.

[0132] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A pollen composition, characterized in that The invention comprises pine pollen, sunflower pollen, buckwheat pollen, corn pollen and rapeseed pollen, wherein the mass ratio of the pine pollen, sunflower pollen, buckwheat pollen, corn pollen and rapeseed pollen is 2-5:1.5-2:1-2:1-2:1-2.

2. The pollen composition according to claim 1, characterized in that The mass ratio of the pine pollen, sunflower pollen, buckwheat pollen, corn pollen and rapeseed pollen is 2-3:2:2:1.5-2:1.5-2.

3. The pollen composition according to claim 2, characterized in that The mass ratio of the pine pollen, sunflower pollen, buckwheat pollen, corn pollen and rapeseed pollen is 3:2:2:1.5:1.

5.

4. A fermentation product, characterized in that Obtained by fermenting the pollen composition according to any one of claims 1 to 3; Preferably, the fermentation product is obtained by fermenting the above-mentioned pollen composition with Ganoderma lucidum; Further preferably, the fermentation product is the supernatant obtained by fermenting the above-mentioned pollen composition with Ganoderma lucidum; More preferably, the fermentation comprises the steps of: Under aseptic operation, the seed culture liquid of Ganoderma lucidum is taken, added into the fermentation medium containing pollen, mixed evenly, cultured, separated, and the supernatant is taken as the fermentation liquid; Preferably, the fermentation medium of the pollen comprises acacia honey, KH2PO4 and MgSO4·7H2O; Further preferably, each 200 mL comprises 1-20 g of acacia honey, 0.1-1 g of KH2PO4, and 0.05-0.5 g of MgSO4·7H2O; More preferably, each 200 mL comprises 10 g of acacia honey, 0.6 g of KH2PO4 and 0.3 g of MgSO4·7H2O.

5. The fermentation product according to claim 4, characterized in that The pollen-containing fermentation medium is prepared according to the following steps: acacia honey, KH2PO4 and MgSO4·7H2O are taken and the volume is adjusted with water.

6. The fermentation product according to claim 5, characterized in that In the fermentation medium containing pollen, the added amount of pollen is 1-100 g / L, more preferably 5-50 g / L, and most preferably 40 g / L.

7. The fermentation product according to claim 4, characterized in that After uniform mixing, the step of adjusting the pH is further included, wherein the pH is adjusted to 3-7, preferably to 5.

8. The fermentation product according to claim 4, characterized in that The method for preparing the seed culture solution of the Ganoderma lucidum species comprises the following steps: Under sterile operation, the activated bacterial strain is inoculated into the basic liquid culture medium, and cultured to obtain the seed culture solution; Preferably, the culture conditions are: 22-28°C, 80-250 r / min shaking culture for 5-15 days; The most preferred condition is: 25°C, 120-180 rpm shaking culture for 5-10 days.

9. Baking powder obtained by drying the fermentation product according to any one of claims 4 to 8.

10. Use of the pollen composition according to any one of claims 1 to 3, the fermentation product according to any one of claims 4 to 8, or the baking powder according to claim 9 in the preparation of a food, health product, or medicine having at least one of antioxidant, anti-inflammatory, anti-aging, and immunity-enhancing effects.

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