Lung-clearing, phlegm-dissolving and cough-relieving composition containing high fru-aspartic pear extract and preparation method and application thereof

Abstract

The present application relates to the technical field of reducing phlegm and relieving cough, and particularly relates to a lung-clearing, phlegm-reducing and cough-relieving composition containing high-Fru-Asp pear essence lotion as well as a preparation method and application thereof. The lung-clearing, phlegm-reducing and cough-relieving composition containing high-Fru-Asp pear essence lotion is prepared from the following ingredients by weight: high-Fru-Asp pear essence lotion 5-50 parts, white kidney bean 10-30 parts and chrysanthemum 25-50 parts. The obtained composition containing high-Fru-Asp pear essence lotion can synergistically improve the lung-clearing, phlegm-reducing and cough-relieving effect, and meanwhile, all the ingredients are raw materials of medicinal and edible origin, thereby improving the use safety.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of relieving cough, and particularly relates to a lung-clearing, phlegm-resolving and cough-relieving composition containing high-Fru-Asp pyridine extract and a preparation method and application thereof. BACKGROUND

[0002] Cough is a protective mechanism of the respiratory tract, which helps to clear irritants and prevent damage. Common types of cough are exogenous cough, which is mainly caused by invasion of exogenous pathogens into the lung system, with wind-cold, wind-heat and wind-dry cough being the most common. The lung prefers wetness and hates dryness, so dryness evil is most likely to harm the lung in autumn. Therefore, it is necessary to first nourish and moisten the lung. The lung is an organ that exchanges gas with the outside world, and lung inflammation easily exposes too many cough receptors in the airway, providing favorable conditions for cough occurrence. At the same time, it also makes sputum viscous and not easy to cough out, leading to frequent cough. Treatment is usually focused on relieving wind and clearing heat, and promoting the lung to stop coughing.

[0003] At present, common cough-relieving products are mainly divided into drugs that reduce stimulation of pharyngeal cough receptors, antibiotics, anti-inflammatory drugs and central antitussive drugs. Sugar syrup drugs such as pear paste can reduce stimulation of pharyngeal cough receptors and have no side effects, which has great advantages.

[0004] Pear paste is a traditional Chinese medicinal diet, which is made by boiling juice of pear or pear and other edible raw materials together, and has the functions of generating body fluid, resolving phlegm, reducing fire, nourishing yin and relieving cough.

[0005] Amadori compound is a relatively stable product in the early stage of Maillard reaction, and Fru-Asp is one of the Amadori compounds and a characteristic Amadori compound in pear paste. The paper "Detection, regulation and function research of Amadori compound in pear paste" of Jiangnan University specifically explores the characteristic Amadori compound Fru-Asp in pear paste, and further explores the functionality of Fru-Asp, including antioxidant, ACE enzyme activity inhibition, cough-relieving ability and application in relieving lung inflammation.

[0006] Chinese patent CN115590911A discloses a high Fru-Asp content cough and expectorant pear syrup and its preparation and component detection method. The preparation method of the invention comprises the following steps: step 1: selection, washing, crushing, pressing, filtering, and obtaining pear juice; step 2: concentrating to a solid content of 55-65%; step 3: maintaining in a closed system at a temperature of 80-90℃ for 3-5h; step 4: rapidly evaporating water to a total solid content of 70-75%; step 5: weighing, filling, sealing, packaging, and obtaining pear syrup. The preparation process of the invention increases the Fru-Asp content in the pear syrup by more than 150%, and the 5-HMF content is only 4.37% of the average value of ordinary pear syrup products, and the cough and expectorant effect is obvious.

[0007] Although there have been many studies on the optimization of the preparation process of pear syrup, the single component of pear syrup has limited lung clearing, phlegm reducing and cough relieving effect, therefore, based on pear syrup, more lung clearing, phlegm reducing and cough relieving products need to be researched, which can achieve better lung clearing, phlegm reducing and cough relieving effect while being natural and healthy. SUMMARY

[0008] The purpose of the present application is to provide a lung clearing, phlegm reducing and cough relieving composition containing high Fru-Asp pear extract and its preparation method and application. The synergistic effect of natural raw materials is utilized to screen out lablab seed, chrysanthemum and high Fru-Asp pear extract for compounding, and it is found that the composition can synergistically improve the lung clearing, cough relieving and phlegm reducing effect, and has significant anti-inflammatory activity, which perfects the therapeutic effect of pear syrup products. At the same time, all the ingredients are medicinal and edible raw materials, which improves the safety of use.

[0009] To achieve the above-mentioned purpose of the application, the technical scheme of the present application is as follows:

[0010] On the one hand, the present application provides a lung clearing, phlegm reducing and cough relieving composition containing high Fru-Asp pear extract, which is made of the following ingredients according to weight parts: high Fru-Asp pear extract 5-50 parts, lablab seed 10-30 parts and chrysanthemum 25-50 parts.

[0011] Chrysanthemum has the effect of clearing heat and resolving toxicity, and lablab seed is a good adjuvant for cough relieving and phlegm reducing products. The combination of the two and high Fru-Asp pear extract for preparing the composition significantly improves the lung clearing, phlegm reducing and cough relieving effect, and achieves good synergistic effect.

[0012] In the present application, high Fru-Asp pear extract means pear syrup with Fru-Asp mass content ≥1.0%.

[0013] Fru-Asp is one of the Amadori compounds, and is also a characteristic Amadori compound in pear syrup. The description of Fru-Asp can be referred to the literature such as the paper "Detection, regulation and function research of Amadori compounds in pear syrup" of Jiangnan University.

[0014] Preferably, in the high Fru-Asp pear essence extract, the mass content of Fru-Asp is ≥1.2%.

[0015] Preferably, the lung-clearing, phlegm-dissolving and cough-relieving composition containing the high Fru-Asp pear essence extract is prepared from the following ingredients by weight: 10-40 parts of high Fru-Asp pear essence extract, 12-20 parts of white kidney bean, and 30-45 parts of chrysanthemum.

[0016] Further preferably, the lung-clearing, phlegm-dissolving and cough-relieving composition containing the high Fru-Asp pear essence extract is prepared from the following ingredients by weight: 20 parts of high Fru-Asp pear essence extract, 15 parts of white kidney bean, and 40 parts of chrysanthemum.

[0017] In another aspect, the application provides a preparation method of the lung-clearing, phlegm-dissolving and cough-relieving composition containing the high Fru-Asp pear essence extract, comprising the following steps:

[0018] (1) The formula amount of chrysanthemum and white kidney bean is crushed, water decoction extraction is performed, filtration is performed, and a filtrate is obtained;

[0019] (2) The filtrate obtained in step (1) is placed at -5°C-5°C for 2h-15h, filtration is performed, the supernatant is taken, and the relative density of the concentrated solution is 1.15-1.2, and a concentrated solution is obtained;

[0020] (3) The concentrated solution obtained in step (2) and the formula amount of high Fru-Asp pear essence extract are mixed to obtain the product.

[0021] Preferably, in step (1), the water decoction extraction is specifically: water decoction extraction is performed 1-3 times, 10-20 times the weight of water is added each time, and decoction extraction is performed for 0.5h-2h; further preferably: water decoction extraction is performed 2 times, 15 times the weight of water is added each time, and decoction extraction is performed for 1h.

[0022] In step (1), the filtration can be a conventional filtration method in the art, and the purpose is to achieve the effect of solid-liquid separation, including but not limited to normal pressure filtration, reduced pressure filtration, etc.

[0023] In step (2), the concentration can be a conventional concentration method in the art, including but not limited to evaporation concentration, membrane separation concentration, reduced pressure concentration, etc.

[0024] Preferably, step (2) is specifically: placing the filtrate obtained in step (1) at 0℃ for 10h, filtering, taking the supernatant, and concentrating under reduced pressure to a relative density of 1.15-1.2 to obtain a concentrated solution.

[0025] For the technical solution of the present application, placing under suitable low temperature conditions to precipitate unnecessary components, and optimizing the composition of the extract, are necessary preparation steps, and the optimal conditions for precipitation are: placing at 0℃ for 10h.

[0026] As a most preferred example of the present application, the following steps are included:

[0027] (1) Pulverizing a formula amount of chrysanthemum and white kidney beans, and adding water to decoct and extract 2 times, each time adding 15 times the weight of water to decoct and extract for 1h, filtering, and combining the filtrates;

[0028] (2) placing the filtrate obtained in step (1) at 0℃ for 10h, filtering, taking the supernatant, and concentrating under reduced pressure to a relative density of 1.15-1.2 to obtain a concentrated solution;

[0029] (3) mixing the concentrated solution obtained in step (2) with a formula amount of high Fru-Asp pear extract, and thoroughly stirring and mixing to obtain a product.

[0030] Preferably, the preparation method of the lung-clearing, phlegm-dissolving, and cough-relieving composition containing high Fru-Asp pear extract can further include the step of freeze-drying the product obtained in step (3) to prepare a powder preparation, according to the needs of the preparation form.

[0031] Preferably, the lung-clearing, phlegm-dissolving, and cough-relieving composition containing high Fru-Asp pear extract can further select suitable raw and auxiliary materials according to the needs of the product type or functional direction, and the raw and auxiliary materials are exemplified by stabilizers, natural pigments, sour taste adjusting agents, sweet taste adjusting agents, thickening agents, emulsifiers, and natural antioxidants, etc.

[0032] Among them, the stabilizers are exemplified by pectin, mannitol, xanthan gum, etc.; the natural pigments are exemplified by cochineal, β-carotene, gardenia powder, etc.; the sour taste adjusting agents are exemplified by DL-malic acid, citric acid, etc.; the sweet taste adjusting agents are exemplified by maltitol, sorbitol, erythritol, steviol glycoside, mogroside, etc.; the thickening agents are exemplified by soybean polysaccharide, sodium caseinate, etc.; the emulsifiers are exemplified by phospholipids, glycerol, etc.; and the natural antioxidants are exemplified by ascorbic acid, tea polyphenols, vitamin E, etc.

[0033] In still another aspect, the present application provides the use of the lung-clearing, phlegm-dissolving, and cough-relieving composition containing high Fru-Asp pear extract in the preparation of a lung-clearing and phlegm-dissolving product.

[0034] Finally, the application provides the use of the lung-clearing, phlegm-dissolving and cough-relieving composition containing the high-Fru-Asp pear essence extract in the preparation of anti-inflammatory and cough-relieving products.

[0035] In the application, the prevention and treatment include the prevention and treatment of cough and excessive sputum. The method for preventing cough from occurring aims to prevent, slow down (reduce) the unwanted physiological changes or pathological changes in the treatment subject, and the beneficial or desired clinical outcomes include but are not limited to avoiding the occurrence of cough and excessive sputum, reducing the symptoms of cough and excessive sputum, reducing the degree of cough and excessive sputum, stabilizing (not worsening) the state of cough and excessive sputum, delaying or slowing down the progression of cough, improving or alleviating the state of cough and excessive sputum, and relieving.

[0036] The subject in need of treatment includes a subject who has suffered from a disease or a subject who is susceptible to a disease or a subject who intends to prevent a disease.

[0037] In the application, the subject of the prevention and treatment is a human or a mammal, and the mammal includes bovine, equine, ovine, porcine, canine, feline, rodent and primate. The rodent is a mouse and / or a rat.

[0038] The application has the following beneficial effects:

[0039] (1) The white hyacinth bean, chrysanthemum and high-Fru-Asp pear essence extract are combined in a reasonable proportion, which shows a synergistic effect on the treatment of lung-clearing, phlegm-dissolving, anti-inflammatory and cough-relieving, and significantly improves the treatment activity.

[0040] (2) The raw materials of the application are all natural ingredients with the same medicinal and edible properties, and are safe to use.

[0041] (3) The application uses the extraction method of water extraction and low-temperature impurity removal to obtain a concentrated solution, and can obtain a synergistic composition. BRIEF DESCRIPTION OF DRAWINGS

[0042] Figure 1 The detection spectrum of the high-Fru-Asp pear essence extract used in the application.

[0043] Figure 2 The detection results of the phenol red excretion of the control group, the positive drug group and the example group are shown in the following figure:

[0044] In the figure, represents p<0.05 compared with the control group, represents p<0.01 compared with the control group.

[0045] Figure 3Figure of the results of the phenol red excretion test for the Example 1 group and the comparative example group;

[0046] In the figure, represents p<0.05 compared with the Example 1 group, represents p<0.01 compared with the Example 1 group.

[0047] Figure 4 Figure of the results of the swelling rate test for the control group, the positive drug group, the Example group and the comparative example group;

[0048] In the figure, represents p<0.05 compared with the control group, represents p<0.01 compared with the control group;

[0049] represents p<0.05 compared with the Example 1 group, and ## represents p<0.01 compared with the Example 1 group. DETAILED DESCRIPTION

[0050] The following non-limiting examples can enable those of ordinary skill in the art to more fully understand the present application, but in no way limit the present application. The following content is only an exemplary description of the scope of the present application, and those skilled in the art can make various changes and modifications to the present application according to the disclosed content, and it should also belong to the scope of the present application.

[0051] The present application will be further described in the following specific examples. The various chemical reagents used in the examples of the present application are obtained by conventional commercial routes unless otherwise specified. The contents described below are mass contents unless otherwise specified. It is understood to be carried out at room temperature unless otherwise specified.

[0052] In the following examples, the relative density of the concentrated solution is measured at 60°C.

[0053] The high Fru-Asp pear essence extract is prepared according to Example 2 of Chinese patent CN115590911A. The Fru-Asp content is 1.2%, the mass spectrometry conditions for detection are shown in Table 1, and the detection spectrum is shown in Figure 1 .

[0054] Table 1

[0055]

[0056] After animal experiment test (the test method is the same as the test method described in the following examples), the results of cough relief, expectorant and anti-inflammatory of the high Fru-Asp pear essence extract are shown in Table 2.

[0057] Table 2

[0058]

[0059] Note: in the table, Represent p < 0.01 compared with the control group.

[0060] The embodiment of the present application is based on the high Fru-Asp pear essence juice prepared in Chinese patent CN115590911A, and the purpose of improving the effect of relieving cough and reducing the dosage of medication while achieving the effect of anti-inflammatory is achieved through the screening and improvement of the formula and process, so as to obtain a compound product with better and more comprehensive activity.

[0061] Example 1

[0062] According to the weight parts, the raw material formula is: high Fru-Asp pear essence juice 20 parts, white kidney bean 15 parts and chrysanthemum 40 parts.

[0063] Preparation process:

[0064] (1) The formula amount of chrysanthemum and white kidney bean is crushed, and water is decocted and extracted twice, each time 15 times the weight of water is added and decocted and extracted for 1 h, filtered, and the filtrate is combined;

[0065] (2) The filtrate obtained in step (1) is placed at 0℃ for 10 h, filtered, and the supernatant is taken, and concentrated under reduced pressure to a relative density of 1.2 to obtain a concentrated solution;

[0066] (3) The concentrated solution obtained in step (2) and the formula amount of high Fru-Asp pear essence juice are mixed, and fully stirred and mixed to obtain the product.

[0067] Example 2

[0068] The difference between example 2 and example 1 is that the raw material formula of example 2 is:

[0069] High Fru-Asp pear essence juice 10 parts, white kidney bean 20 parts and chrysanthemum 45 parts.

[0070] The preparation process is the same as example 1.

[0071] Example 3

[0072] The difference between example 3 and example 1 is that the raw material formula of example 3 is:

[0073] High Fru-Asp pear essence juice 40 parts, white kidney bean 12 parts and chrysanthemum 30 parts.

[0074] The preparation process is the same as example 1.

[0075] Example 4

[0076] The raw material formula of Example 4 is as follows in terms of parts by weight, different from Example 1:

[0077] High Fru-Asp pear essence extract 5 parts, white lentil 30 parts and chrysanthemum 50 parts.

[0078] The preparation process is the same as Example 1.

[0079] Example 5

[0080] The raw material formula of Example 5 is as follows in terms of parts by weight, different from Example 1:

[0081] High Fru-Asp pear essence extract 50 parts, white lentil 10 parts and chrysanthemum 25 parts.

[0082] The preparation process is the same as Example 1.

[0083] Example 6

[0084] Step (2) of Example 6 is as follows, different from Example 1: the filtrate obtained in step (1) is placed at -5℃ for 2h, and after being taken out, it is placed at room temperature for a while, filtered, the supernatant is taken, and concentrated under reduced pressure to a relative density of 1.2 to obtain a concentrated solution.

[0085] Example 7

[0086] Step (2) of Example 7 is as follows, different from Example 1: the filtrate obtained in step (1) is placed at 5℃ for 15h, filtered, the supernatant is taken, and concentrated under reduced pressure to a relative density of 1.2 to obtain a concentrated solution.

[0087] Comparative Example 1

[0088] The raw material formula of Comparative Example 1 is as follows in terms of parts by weight, different from Example 1:

[0089] High Fru-Asp pear essence extract 20 parts, white lentil 15 parts.

[0090] The preparation process is the same as Example 1.

[0091] Comparative Example 2

[0092] The raw material formula of Comparative Example 2 is as follows in terms of parts by weight, different from Example 1:

[0093] High Fru-Asp pear essence extract 20 parts, chrysanthemum 40 parts.

[0094] The preparation process is the same as Example 1.

[0095] Comparative Example 3

[0096] The raw material formula of Comparative Example 3 is as follows in terms of parts by weight, different from Example 1:

[0097] High Fru-Asp pear essence extract 20 parts, white beans 15 parts and silver ear 40 parts.

[0098] The preparation process is the same as Example 1.

[0099] Comparative Example 4

[0100] The raw material formula of Comparative Example 4 is different from Example 1, and is as follows by weight:

[0101] High Fru-Asp pear essence extract 20 parts, monk fruit 15 parts and orange peel 40 parts.

[0102] The preparation process is the same as Example 1.

[0103] Comparative Example 5

[0104] The raw material formula is the same as Example 1, and the preparation process is as follows:

[0105] (1) The formula amount of chrysanthemum and white beans is crushed, and water is decocted and extracted twice, each time 15 times the weight of water is added and decocted and extracted for 1 h, filtered, and the filtrates are combined;

[0106] (2) The filtrate obtained in step (1) is filtered, and the supernatant is taken and concentrated under reduced pressure to a relative density of 1.2 to obtain a concentrated solution;

[0107] (3) The concentrated solution obtained in step (2) and the formula amount of high Fru-Asp pear essence extract are mixed, and fully stirred and mixed to obtain the product.

[0108] Results of detection:

[0109] 1. Cough effect detection

[0110] Experimental animals: healthy Kunming mice (18-22 g), half male and half female.

[0111] Pre-experimental screening: the mice were placed in an inverted 500 mL glass beaker, and the cotton ball method was used to expose the mice to 0.2 mL of 25% concentrated ammonia water for 45 s. The cough latency period was less than 1 min and the number of coughs within 1 min was > 3 times, which was considered qualified, otherwise it was eliminated. The cough standard is: abdominal muscle contraction, mouth opening, sometimes accompanied by coughing. The qualified mice were randomly divided into groups, 10 mice per group, and the mice were given medicine every other day.

[0112] Experimental grouping and drug administration:

[0113] Positive drug group: intragastrically administered with dextromethorphan hydrobromide, once a day, with a dosage of 30 mg / kg, an intragastric volume of 20 mL / kg, and continuous administration for 5 days;

[0114] Experimental group: high Fru-Asp pear essence lotion, products prepared by example 1-7, and comparative examples 1-5 were administered respectively, once a day, the administration dose was 2g / kg, the intragastric volume was 20mL / kg, and the continuous administration lasted for 5 days;

[0115] Control group: the same volume of normal saline was administered intragastrically.

[0116] Experimental method:

[0117] One hour after the last administration, the mice were placed in an inverted 500mL glass beaker, and the cotton ball method was used to expose the mice to 0.2mL of 25% concentrated ammonia water for 45s. The cough latency (the time from stimulation to the first cough) was recorded in seconds (s); the number of coughs within 2min was recorded.

[0118] The results are shown in Table 3.

[0119] Table 3

[0120]

[0121] Note: in the table, p<0.05 compared with the control group, p<0.01 compared with the control group.

[0122] The results show that the products prepared by examples 1-7 of the present application can significantly prolong the cough latency and reduce the number of coughs compared with the control group. Compared with the high Fru-Asp pear essence lotion administration group, the cough-relieving level is also improved to a certain extent. Comparative examples 1-7 and the control group also show significant differences, but from the specific data level, there are still significant differences compared with example 1.

[0123] 2. Effect of expectorant detection

[0124] Experimental animals: healthy Kunming mice (18g-22g), half male and half female, and the mice were not allowed to eat but allowed to drink water for 16h before the experiment.

[0125] Experimental grouping and administration:

[0126] Positive drug group: hydrochloric acid ambroxol oral solution (Hualvansanjiuye, State Drug Standard Code H20103009) was administered intragastrically, once a day, the administration dose was 6.15mL / kg, the intragastric volume was 20mL / kg, and the continuous administration lasted for 5 days;

[0127] Experimental group: high Fru-Asp pear essence lotion, products prepared by example 1-7, and comparative examples 1-5 were administered respectively, once a day, the administration dose was 2g / kg, the intragastric volume was 20mL / kg, and the continuous administration lasted for 5 days;

[0128] Control group: intragastric administration of the same volume of normal saline.

[0129] Experimental method:

[0130] 1h after the last drug, intraperitoneal injection of 0.5% phenol red solution 0.5 mL, 30 min after killing mice, separation of the trachea between the thyroid cartilage to the main bronchus, put into test tube, add 1 mL 5% NaHCO3 normal saline solution, ultrasonic treatment for 10 min, using multifunctional microplate reader to determine the absorbance OD value at 546 nm.

[0131] Preparation of 10 μg / mL, 5 μg / mL, 2.5 μg / mL, 1 μg / mL, 0.5 μg / mL, 0.25 μg / mL, 0.1 μg / mL phenol red 5% NaHCO3 normal saline solution, determine its absorbance OD value at 546 nm, draw phenol red standard curve, calculate the phenol red excretion (μg / mL) and the increase rate of secretion (%).

[0132] The increase rate of secretion = (experimental group phenol red secretion - control group phenol red secretion) / control group phenol red secretion x 100%.

[0133] The results are shown in Figure 2 , Figure 3 .

[0134] From Figure 2 The results can be seen, the product prepared by the embodiment of the application can significantly increase the phenol red secretion, indicating that the composition can better promote the secretion of pharyngeal and bronchial mucus, and achieve good expectorant effect. Compared with the high Fru-Asp pear essence lotion administration group (table 2), the expectorant effect is significantly improved, and the disadvantage of limited treatment activity of high Fru-Asp pear essence lotion is solved.

[0135] Figure 3 The results show that the phenol red secretion of the comparative examples 1-5 has a significant difference compared with example 1, and the increase rate of secretion is also very different. Among them, the formula is screened in comparative examples 1-4, and the results show that the combination of high Fru-Asp pear essence lotion, white kidney bean and chrysanthemum can further improve the expectorant effect of Fru-Asp pear essence lotion and reduce the dosage compared with other conventional combinations. The preparation process of comparative example 5 does not have a low temperature treatment step, and compared with example 1, the results show that appropriate low temperature treatment can significantly improve the impurity removal effect, optimize the composition of the composition, and thus significantly improve the expectorant activity of the obtained product.

[0136] 3. Anti-inflammatory effect detection

[0137] Experimental animals: healthy Kunming mice (18g-22g), half male and half female.

[0138] Experimental grouping and administration:

[0139] Positive drug group: intragastrically administered prednisone 5 mg / kg, once a day, intragastric volume 20 mL / kg, continuous administration for 3 days;

[0140] Experimental group: respectively administered the products prepared by example 1 to example 7, comparative example 1 to comparative example 5, once a day, administration dose 2 g / kg, intragastric volume 20 mL / kg, continuous administration for 3 days;

[0141] Control group: intragastrically administered the same volume of normal saline.

[0142] Experimental method:

[0143] 1 hour after the last administration, the experimental mice were respectively smeared with 0.05 mL of xylene to cause inflammation on the right ear inner and outer sides, 1 hour later, the mice were executed by cervical dislocation, and the left and right ear pieces were respectively taken from the same part with an 8mm puncher, weighed, and the swelling rate was calculated.

[0144] Swelling rate = (right ear weight - left ear weight) / left ear weight x 100%.

[0145] The results are shown in Figure 4

[0146] Figure 4 The results show that the combination of high Fru-Asp pear essence extract, white lentil and chrysanthemum achieves good anti-inflammatory activity. The results of comparative example 1 to comparative example 4 show that the combination of high Fru-Asp pear essence extract, white lentil and chrysanthemum achieves better anti-inflammatory activity. Specifically, the anti-inflammatory activity of example 1 and comparative example shows significant difference. And the comparison results of comparative example 5 and example 1 show that appropriate low temperature treatment also improves the anti-inflammatory activity of the obtained product.

[0147] The above only describes the preferred embodiments of the present application and is not intended to limit the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.​

Claims

1. A lung-clearing, phlegm-reducing, and cough-relieving composition containing high Fru-Asp pear extract, characterized in that, According to the weight, it is made from the following ingredients: 5-50 parts high-Fru-Asp pear extract, 10-30 parts white lentils and 25-50 parts chrysanthemum; The high-Fru-Asp pear extract is a pear paste with a Fru-Asp content ≥1.0% by mass; The method for preparing the composition includes the following steps: (1) The prescribed amount of chrysanthemum and white hyacinth bean are crushed, boiled with water to extract, and filtered to obtain the filtrate; (2) Place the filtrate obtained in step (1) at -5℃~5℃ for 2-15h, filter, take the supernatant, and concentrate it to a relative density of 1.15-1.2 to obtain the concentrate; (3) Mix the concentrate obtained in step (2) with the formula amount of high Fru-Asp pear extract to obtain the product.

2. The lung-clearing, phlegm-reducing, and cough-relieving composition containing high Fru-Asp pear extract according to claim 1, characterized in that, The high-Fru-Asp pear extract contains ≥1.2% Fru-Asp.

3. The lung-clearing, phlegm-reducing, and cough-relieving composition containing high Fru-Asp pear extract according to claim 2, characterized in that, It is made from the following ingredients by weight: 10-40 parts high-Fru-Asp pear extract, 12-20 parts white lentils and 30-45 parts chrysanthemum.

4. The lung-clearing, phlegm-reducing, and cough-relieving composition containing high Fru-Asp pear extract according to claim 3, characterized in that, It is made from the following ingredients by weight: 20 parts high-Fru-Asp pear extract, 15 parts white lentils and 40 parts chrysanthemum.

5. A method for preparing the lung-clearing, phlegm-reducing, and cough-relieving composition containing high Fru-Asp pear extract as described in any one of claims 1-4, characterized in that, Includes the following steps: (1) The prescribed amount of chrysanthemum and white hyacinth bean are crushed, boiled with water to extract, and filtered to obtain the filtrate; (2) Place the filtrate obtained in step (1) at -5℃~5℃ for 2-15h, filter, take the supernatant, and concentrate it to a relative density of 1.15-1.2 to obtain the concentrate; (3) Mix the concentrate obtained in step (2) with the formula amount of high Fru-Asp pear extract to obtain the product.

6. The preparation method according to claim 5, characterized in that, In step (1), the extraction by boiling water specifically involves boiling water 1-3 times, adding 10-20 times the weight of water each time and boiling for 0.5-2 hours.

7. The preparation method according to claim 5, characterized in that, Step (2) specifically involves: placing the filtrate obtained in step (1) at 0°C for 10 hours, filtering, taking the supernatant, and concentrating it under reduced pressure to a relative density of 1.15-1.2 to obtain the concentrate.

8. The preparation method according to claim 5, characterized in that, Includes the following steps: (1) The amount of chrysanthemum and white hyacinth bean in the formula is crushed, and water is added to decoct and extract twice. Each time, 15 times the weight of water is added and the decoction is decocted and extracted for 1 hour. The mixture is filtered and the filtrates are combined. (2) Place the filtrate obtained in step (1) at 0°C for 10 hours, filter, take the supernatant, and concentrate under reduced pressure to a relative density of 1.15-1.2 to obtain the concentrate; (3) Mix the concentrated liquid obtained in step (2) with the formula amount of high Fru-Asp pear extract, stir thoroughly to obtain the product.

9. The preparation method according to any one of claims 5-8, characterized in that, It also includes the step of freeze-drying the product obtained in step (3) to prepare a powder formulation. ‌ 10. The use of the lung-clearing, phlegm-reducing, and cough-relieving composition containing high Fru-Asp pear extract as described in any one of claims 1-4 in the preparation of lung-clearing and phlegm-reducing products.

11. The use of the lung-clearing, phlegm-reducing, and cough-relieving composition containing high Fru-Asp pear extract as described in any one of claims 1-4 in the preparation of anti-inflammatory and cough-relieving products.

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