Traditional Chinese medicine sachet for preventing influenza and preparation method thereof

Abstract

The invention belongs to the technical field of traditional Chinese medicine application, and particularly relates to a traditional Chinese medicine sachet for preventing influenza and a preparation method thereof.The preparation method comprises the following steps that rhizoma atractylodis, pogostemon cablin, fortune eupatorium herb, rhizoma acori graminei and folium artemisiae argyi which are equal in mass are weighed, volatile oil is extracted, and traditional Chinese medicine compound volatile oil is obtained; modifying the taste to obtain modified traditional Chinese medicine compound volatile oil; dissolving the flavoring traditional Chinese medicine compound volatile oil in absolute ethyl alcohol to obtain a traditional Chinese medicine compound volatile oil ethanol solution; a traditional Chinese medicine compound volatile oil ethanol solution is added into a beta-cyclodextrin saturated aqueous solution for an inclusion reaction, the traditional Chinese medicine compound volatile oil ethanol solution serves as a core material, and the beta-cyclodextrin saturated aqueous solution serves as a wall material. According to the prepared traditional Chinese medicine sachet, volatile oil in traditional Chinese medicine materials is extracted, the volatile oil is microencapsulated through beta-cyclodextrin, and the mass and the size are reduced. The weight of the traditional Chinese medicine sachet for preventing influenza is 0.02-0.1 g, the volume is about 0.04 cm < 3 >, and the traditional Chinese medicine sachet is easier to carry and use.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicine application, and particularly relates to a traditional Chinese medicine sachet for preventing influenza and a preparation method thereof. Background Art

[0002] Influenza, commonly known as the flu, is caused by the influenza virus. Traditional Chinese medicine (TCM) has a long history of understanding and preventing and treating epidemic diseases, accumulating rich and valuable clinical experience. Topical Chinese medicine for preventing epidemic diseases is widely accepted due to its simplicity, low cost, and lack of significant side effects, making it feasible and practical. Yiguan therapy, also known as yiguan therapy, is a topical treatment that uses the aromatic volatiles of the herbs, their inherent therapeutic effects, and the stimulation of local meridian points to prevent and treat diseases. Modern research has shown that incense therapy has certain effects in killing bacteria and viruses, promoting metabolism, and boosting immunity. Numerous clinical studies on Chinese herbal sachets have demonstrated significant efficacy in preventing epidemic diseases such as seasonal influenza in adults, influenza A (H1N1), childhood colds, and hand, foot and mouth disease, reducing the prevalence of these diseases. They are also effective in alleviating upper respiratory symptoms, including nasal congestion, runny nose, and headaches. They can also improve appetite, mood, sleep, fatigue, and cold symptoms.

[0003] In the prior art "Zhu Feifei, Hu Di, Qin Yuge. A comparative study on the effectiveness of Chinese herbal sachets in preventing colds in primary school students [J]. Bulletin of Traditional Chinese Medicine, 2023, 22(4): 61-6268", Chinese herbal medicines for preventing influenza are ground into powder and then directly made into Chinese herbal sachets. Since the content of volatile oil in Chinese herbal medicines is usually low, traditional sachets require a large amount of medicine to achieve a certain drug concentration. Therefore, the prepared Chinese herbal sachets are often large in size and inconvenient to carry around. Therefore, there is an urgent need to provide a new method for preparing Chinese herbal sachets. Summary of the Invention

[0004] The purpose of the present invention is to provide a method for preparing a Chinese medicine sachet for preventing influenza, which solves the defect that the Chinese medicine sachet for preventing influenza in the prior art is large in size and inconvenient to carry around.

[0005] The technical solution adopted in the present invention is: The present invention provides a method for preparing a Chinese medicine sachet for preventing influenza, comprising the following steps: Equal masses of atractylodes lancea, patchouli, perilla frutescens, calamus tatarinowii, and artemisia argyi are weighed, mixed, and then crushed to extract volatile oil to obtain a traditional Chinese medicine compound volatile oil; the traditional Chinese medicine compound volatile oil is flavored to obtain a flavored traditional Chinese medicine compound volatile oil; the flavored traditional Chinese medicine compound volatile oil is dissolved in anhydrous ethanol to obtain an ethanol solution of the traditional Chinese medicine compound volatile oil; a saturated aqueous solution of β-cyclodextrin is prepared; the ethanol solution of the traditional Chinese medicine compound volatile oil is added to the saturated aqueous solution of β-cyclodextrin at a core-to-wall ratio of 1:2 to 1:6 to carry out an inclusion reaction, wherein the ethanol solution of the traditional Chinese medicine compound volatile oil serves as a core material and the saturated aqueous solution of β-cyclodextrin serves as a wall material. After the inclusion reaction is completed, a traditional Chinese medicine sachet for preventing influenza is obtained.

[0006] Preferably, the core-to-wall ratio is 1:3 to 1:5.

[0007] Preferably, the core-to-wall ratio is 1:4.

[0008] Preferably, the inclusion reaction is carried out under ultrasonication at 40° C. for 60 min.

[0009] Preferably, when the Chinese herbal compound volatile oil is flavored, the essential oil used for flavoring includes any one or more of lavender essential oil, lemon essential oil, citrus essential oil, osmanthus essential oil, jasmine essential oil, white jasmine essential oil, rose essential oil and sandalwood essential oil; the volume ratio of the flavoring essential oil to the Chinese herbal compound volatile oil is 3:1.

[0010] Preferably, the volume ratio of the flavor-correcting Chinese herbal compound volatile oil to anhydrous ethanol is 1:2.

[0011] Preferably, after the synthesis reaction is completed, refrigeration, filtration, washing and freeze-drying are further performed.

[0012] Preferably, the refrigeration time is 24 hours.

[0013] Preferably, the method further comprises solidifying the influenza-preventing Chinese medicine sachet; the solidification process is: mixing jojoba oil, coconut oil and beeswax, and then mixing the mixture with the influenza-preventing Chinese medicine sachet.

[0014] The present invention also provides a traditional Chinese medicine sachet for preventing influenza prepared by the preparation method.

[0015] The beneficial effects of the present invention are: The present invention provides a method for preparing a traditional Chinese medicine sachet for preventing influenza, comprising the following steps: weighing equal masses of atractylodes lancea, patchouli, perilla, calamus tatarinowii, and wormwood, mixing and crushing the mixture, extracting volatile oil, and obtaining a traditional Chinese medicine compound volatile oil; correcting the flavor of the traditional Chinese medicine compound volatile oil to obtain a corrected traditional Chinese medicine compound volatile oil; dissolving the corrected traditional Chinese medicine compound volatile oil in anhydrous ethanol to obtain a traditional Chinese medicine compound volatile oil ethanol solution; preparing a saturated aqueous solution of β-cyclodextrin; adding the traditional Chinese medicine compound volatile oil ethanol solution to the saturated aqueous solution of β-cyclodextrin at a core-to-wall ratio of 1:2 to 1:6 for inclusion reaction, wherein the traditional Chinese medicine compound volatile oil ethanol solution serves as the core material and the saturated aqueous solution of β-cyclodextrin serves as the wall material; completing the inclusion reaction, and obtaining a traditional Chinese medicine sachet for preventing influenza. The traditional Chinese medicine sachet for preventing influenza prepared by the present invention extracts volatile oil from traditional Chinese medicine materials by distillation, and uses β-cyclodextrin to microencapsulate the volatile oil, thereby reducing mass and volume. The weight of traditional sachets is generally between 20g and 50g, and the volume is 50cm 3 ~200cm 3 The weight of the Chinese medicine sachet for preventing influenza prepared by the present invention is 0.02g~0.1g and the volume is about 0.04cm 3 , easier to carry and use.

[0016] The traditional Chinese medicine sachet for preventing influenza prepared by the present invention is prepared by extracting volatile oils from the traditional Chinese medicinal materials Atractylodes lancea, Patchouli, Eupatorium fortunei, Acorus calamus, and Artemisia argyi, thereby improving the utilization efficiency of the volatile oils. The volatile oils are microencapsulated using β-cyclodextrin. The constructed microcapsules have a sustained-release effect, and the release time of the volatile oils can be sustained for more than 60 days, avoiding the problem of frequent drug changes and making it more convenient to use. At the same time, due to the sustained-release effect of the microcapsules, the drug ingredients are slowly and continuously released, avoiding the problem of high initial concentration and strong irritation of traditional sachets or products containing only essential oils, thereby improving the adaptability of the product.

[0017] The main active ingredient of the traditional Chinese medicine sachet for preventing influenza prepared by the present invention is the volatile oil in the compound preparation. Volatile oil is also one of the main active ingredients of most Chinese medicinal materials and is an important material basis for the clinical efficacy of traditional Chinese medicine. However, due to the unstable physical and chemical properties of volatile oil, the quality control difficulty of related preparations in the prior art is increased, which limits the further development and promotion of the preparation. The microencapsulation of volatile oil in the present invention can improve the stability of volatile oil and achieve a slow and controlled release of volatile oil. There is no research on the improvement of modern preparations of traditional Chinese medicine sachets in the prior art. Therefore, the present invention explores the research of microencapsulated preparations of traditional Chinese medicine sachets, providing diversified options for the prevention and treatment of influenza.

[0018] In the present invention, β-cyclodextrin is very suitable for the microencapsulation of essential oils. The microcapsules constructed by it can improve the light resistance, thermal stability and moisture stability of essential oils, make them have a sustained release effect, and are easy to store and transport, and have low cost. Therefore, the present invention chooses to use β-cyclodextrin as a microencapsulation carrier, in order to microencapsulate traditional Chinese medicine sachets, achieve miniaturization of the sachets, and achieve controllable concentration and long-lasting release of the sachets, so that it can better meet the epidemic prevention needs of modern people.

[0019] The present invention also innovatively explores flavor correction research for volatile oils in sachets, using natural plant essential oils such as lavender oil and lemon oil to correct the flavor of the volatile oils. Traditional Chinese medicines have a strong odor and a limited audience, making them unsuitable for use in workplaces, schools, and public places. Therefore, the present invention utilizes natural plant essential oils such as lavender oil and lemon oil, which are commonly used in daily chemical products, to conduct appropriate flavor correction research on the volatile oils. This makes the odor of the volatile oils more acceptable and usable, which is conducive to the further promotion of flu-prevention Chinese medicine sachets.

[0020] The influenza prevention Chinese medicine sachet prepared by the present invention is very small in size and can be worn on a mask through a magnetic aromatherapy buckle. On the one hand, it is beneficial to the efficacy of the medicine, and at the same time, it can also reduce the impact on the surrounding environment. It is not restricted by clothing and has a wider range of application scenarios. In addition, it can also be applied to clothing by coating it on an adhesive sticker, which is more adaptable to children and the elderly, and is more conducive to the promotion and use of influenza prevention sachets. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 The compound essential oil β-cyclodextrin microspheres prepared in Example 1, A is the compound essential oil β-cyclodextrin microspheres placed in a bottle; B is the compound essential oil β-cyclodextrin microspheres placed in a culture dish.

[0022] Figure 2 Different usage forms of the traditional Chinese medicine sachets for preventing influenza prepared in Example 1: A is a solid sachet prepared by adding compound essential oil β-cyclodextrin microspheres to a matrix such as beeswax; B is a solid sachet prepared by adding spherical compound essential oil β-cyclodextrin microspheres to a matrix such as beeswax; C is a compound essential oil β-cyclodextrin microsphere fixed in an aromatherapy buckle; D is a compound essential oil β-cyclodextrin microsphere not fixed in the aromatherapy buckle; E is a compound essential oil β-cyclodextrin microsphere worn on a mask.

[0023] Figure 3 The Chinese medicine sachet for preventing influenza is applied to the adhesive patch. A and C are blank groups; B is coated with the Chinese medicine sachet for preventing influenza prepared in Comparative Example 2; and D is coated with the Chinese medicine sachet for preventing influenza prepared in Example 1.

[0024] Figure 4This is a graph showing the particle size determination of the compound essential oil β-cyclodextrin microspheres prepared in Example 1.

[0025] Figure 5 UV scanning spectrum.

[0026] Figure 6 This is a test of the embedding efficiency of the compound essential oil β-cyclodextrin microspheres prepared in Examples 1 to 5. DETAILED DESCRIPTION

[0027] In order to enable those skilled in the art to better understand and implement the technical solution of the present invention, the present invention is further described below with reference to specific embodiments.

[0028] The inventive concept of the present invention is as follows: Modern research suggests that Chinese herbal sachets can directly kill pathogens or eliminate them by regulating human functions, protecting the body from pathogenic attack. The sachets emit a persistent aromatic scent, creating a high-concentration microenvironment around the mouth and nose. Their active ingredients can repel various bacteria and viruses, stimulate the nervous system, and stimulate the respiratory mucosa, leading to the continuous production of secretory immunoglobulins, strengthening the immune barrier, and inactivating viruses, bacteria, and other microorganisms in the respiratory mucosa, thereby preventing the spread of disease.

[0029] The existing technology usually directly fills or grinds traditional Chinese medicine herbs for preventing influenza into powder and then makes them into traditional Chinese medicine sachets. The efficacy of traditional Chinese medicine sachets comes from the diffusion of volatile components in the herbs or powders. Since the contact surface between the herbs or powders and the air is limited, the duration of the efficacy is usually short. The shelf life of traditional Chinese medicine sachets is generally only about one week, and there is no obvious medicinal odor afterwards. Therefore, they need to be replaced frequently, which is troublesome to use. The efficiency of the use of traditional Chinese medicine is low and it is easy to cause waste. In addition, the herbs or powders are directly exposed to the air and are easily affected by the environment and become damp and moldy. Improper storage and transportation of traditional Chinese medicine sachets will affect the efficacy of the sachets.

[0030] The existing Chinese medicine sachets are made by hanging ropes, etc. Most of the styles of Chinese medicine sachets are relatively traditional and difficult to match with modern people's clothing. For children who need to exercise more, there are certain safety hazards because they need to hang ropes, etc., which is not conducive to the promotion of wearing incense.

[0031] Based on this, the present invention provides a preparation method of a traditional Chinese medicine sachet for preventing influenza, comprising the following steps: weighing equal masses of atractylodes lancea, patchouli, perilla, calamus tatarinowii and wormwood, mixing and then crushing, extracting volatile oil to obtain a traditional Chinese medicine compound volatile oil; correcting the flavor of the traditional Chinese medicine compound volatile oil to obtain a flavor-corrected traditional Chinese medicine compound volatile oil; dissolving the flavor-corrected traditional Chinese medicine compound volatile oil in anhydrous ethanol to obtain a traditional Chinese medicine compound volatile oil ethanol solution; preparing a saturated aqueous solution of beta-cyclodextrin; adding the traditional Chinese medicine compound volatile oil ethanol solution to the saturated aqueous solution of beta-cyclodextrin at a core-to-wall ratio of 1:2 to 1:6 to carry out an inclusion reaction, wherein the traditional Chinese medicine compound volatile oil ethanol solution serves as a core material and the saturated aqueous solution of beta-cyclodextrin serves as a wall material, and after the inclusion reaction is completed, the traditional Chinese medicine sachet for preventing influenza is obtained.

[0032] The present invention selects a combination of Atractylodes lancea, Patchouli, Elephanthus chinensis, Acorus calamus, and Artemisia argyi. The combination of these herbs can dispel wind pathogens, dry dampness and eliminate turbidity, and awaken the mind. Among them, the monarch herbs are Atractylodes lancea and Artemisia argyi; the ministerial herbs are Patchouli and Elephanthus chinensis; and the adjuvant is Acorus calamus. The ingredients of the present invention are relatively simple, the Chinese medicinal materials cooperate with each other, there is no obvious conflict between the herbs, and no need for the herbs to be used as adjuvants. The effects of the various Chinese medicinal ingredients are as follows: Atractylodes: Atractylodes has a fragrant taste and can eliminate dampness and turbidity inside the body and disperse rheumatism outside. Ancient medical books record that it "is the best at expelling foul and evil air, so it is often used to treat epidemic diseases." It is an important traditional Chinese medicine for epidemic prevention.

[0033] Patchouli: Patchouli has a fragrant, slightly warm, and pungent flavor. It enters the spleen, stomach, and lung meridians, and has the effects of aromatically removing dampness and relieving summer heat. Patchouli primarily contains volatile oils, which have anti-pathogenic effects.

[0034] Perilla: Perilla has a fragrant smell, spicy taste, and a neutral nature. It enters the spleen and stomach meridians. It has the effects of aromatic dehumidification, invigorating the spleen and appetite, and relieving heat. It has a preventive effect on respiratory diseases and a refreshing effect.

[0035] Acorus calamus: Acorus calamus is pungent and fragrant, can clear away phlegm, refresh the mind and improve intelligence. It has the effects of detoxifying and expelling evil spirits, and is a commonly used medicine for preventing diseases in ancient times.

[0036] Mugwort: Mugwort is pungent and warm, and has the effects of warming the meridians to stop bleeding, dispelling cold and relieving pain, and having a fragrant aroma to dispel odor. Mugwort is rich in volatile essential oils, which have a certain inhibitory effect on influenza viruses.

[0037] In the description of the present invention, unless otherwise specified, all reagents used are commercially available and all methods used are conventional techniques in the art. Example

[0038] A method for preparing a Chinese medicine sachet for preventing influenza, comprising the following steps: S1. Extract the volatile oil of the traditional Chinese medicine compound as follows: 5g each of Atractylodes lancea, Patchouli, Cyperus rotundus, Acorus calamus and Artemisia argyi were selected, mixed, crushed and passed through a 60-mesh sieve to collect the fine powder to obtain a compound preparation. The volatile oil of the compound preparation was extracted by steam distillation to obtain a traditional Chinese medicine compound volatile oil.

[0039] S2. Correcting the flavor of the Chinese herbal compound volatile oil to obtain the corrected Chinese herbal compound volatile oil, as follows: Considering that the traditional Chinese medicine compound volatile oil extracted by the above method has a strong traditional Chinese medicine smell, which is not well accepted by some people in terms of sensory experience, it is necessary to correct the flavor of the traditional Chinese medicine compound volatile oil to improve sensory comfort. In this embodiment, lavender essential oil is selected for flavor correction. The traditional Chinese medicine compound volatile oil and lavender essential oil are mixed in a volume ratio of 3:1 for flavor correction to obtain a flavored traditional Chinese medicine compound volatile oil.

[0040] S3. Prepare a Chinese medicine sachet for preventing influenza as follows: The volatile oil of the flavor-correcting Chinese herbal compound is measured and dissolved in anhydrous ethanol in an amount twice its volume to obtain an ethanol solution of the volatile oil of the Chinese herbal compound.

[0041] Weigh β-cyclodextrin and dissolve it in distilled water at a ratio of 1 g:15 mL. Ultrasonicate at 40° C. for 30 min to obtain a saturated aqueous solution of β-cyclodextrin.

[0042] According to the core-wall ratio of 1:4, the ethanol solution of the Chinese herbal compound volatile oil is slowly dripped into the saturated aqueous solution of β-cyclodextrin, and ultrasonic treatment is carried out. The ultrasonic treatment is carried out at 40℃ for 60 minutes to fully include the ethanol solution of the Chinese herbal compound volatile oil as the core material and the saturated aqueous solution of β-cyclodextrin as the wall material; after being taken out, it is placed in the refrigerator for 24 hours, and then filtered with qualitative filter paper and a vacuum pump, washed with a small amount of ethyl acetate three times to wash away the unincluded essential oil, and freeze-dried to obtain white powder microspheres. The white powder microspheres are compound essential oil β-cyclodextrin microspheres, that is, Chinese herbal sachets for preventing influenza, such as Figure 1 shown.

[0043] For easy portability, the compound essential oil β-cyclodextrin microspheres are solidified by the following method: (1) Jojoba oil, coconut oil and beeswax were mixed at a volume ratio of 5:5:2 at 60°C to obtain a base essential oil. The base essential oil and compound essential oil β-cyclodextrin microspheres were added to the base essential oil at a mass ratio of 1:1, and the mixture was evenly mixed. The mixture was cooled to room temperature to obtain a solid Chinese medicine sachet for preventing influenza.

[0044] In actual application, the sachet can be cut according to the container of the sachet. For example, the solid Chinese medicine sachet for preventing influenza can be cut into round pieces with a diameter of 1 cm and a thickness of 3 mm, and placed in a regular mask aromatherapy buckle for wearing. Alternatively, the solid Chinese medicine sachet for preventing influenza can be coated on the adhesive sticker and worn on the body.

[0045] (2) Take 200 mg of the prepared compound essential oil β-cyclodextrin microspheres and directly compress them into tablets. Take a 7 mm tableting mold and apply a pressure of 0.2 tons for 5 seconds to obtain a solid Chinese medicine sachet for preventing influenza. The sachet can be placed in a conventional mask aromatherapy buckle for wearing. Figure 2 shown.

[0046] (3) Take 100 mg of the prepared compound essential oil β-cyclodextrin microspheres and apply them on the adhesive backing and wear them on your body. Figure 3 shown.

[0047] It should be noted that in S1 of Example 1, the Chinese herbal compound volatile oil can also be obtained by mixing the corresponding Chinese medicine essential oils extracted by supercritical carbon dioxide in equal proportions, and the ultimate goal is to obtain the Chinese herbal compound volatile oil of the corresponding Chinese medicine; the essential oil used for flavor correction in S2 can also be any one or more of lemon essential oil, citrus essential oil, osmanthus essential oil, jasmine essential oil, white jasmine essential oil, rose essential oil and sandalwood essential oil, and the purpose of their addition is to enhance sensory comfort, which is similar to the effect produced by lavender essential oil.

[0048] Example 2 to Example 5 A method for preparing a Chinese medicine sachet for preventing influenza is as follows: In Examples 2 to 5, except for the different core-to-wall ratios, other experimental processes are exactly the same as in Example 1. Traditional Chinese medicine sachets for preventing influenza are obtained through this preparation method.

[0049] The core-to-wall ratios of Examples 1 to 5 are shown in Table 1.

[0050] Table 1 Core-to-wall ratio of Examples 1 to 5 Example Core-to-wall ratio Example 1 1：4 Example 2 1：2 Example 3 1：3 Example 4 1：5 Example 5 1：6 In order to demonstrate the effect of the present invention, the inventors set up a comparative example as follows: Comparative Example 1 A method for preparing a Chinese medicine sachet for preventing influenza is as follows: 5 g each of Atractylodes lancea, Patchouli, Cymbidium orbiculare cyperus, Acorus calamus and Artemisia argyi were selected and directly put into a 8 cm × 10 cm linen sachet to obtain a traditional Chinese medicine sachet for preventing influenza.

[0051] Comparative Example 2 A method for preparing a Chinese medicine sachet for preventing influenza is as follows: 5 g each of Atractylodes lancea, Patchouli, Cymbidium orbiculare cyperus, Acorus calamus and Artemisia argyi were selected, mixed and then pulverized, and the mixture was evenly mixed through a 60-mesh sieve. The fine powder was collected and first added into a non-woven filter bag. The non-woven filter bag containing the fine powder was placed into an 8 cm × 10 cm linen sachet to obtain a traditional Chinese medicine sachet for preventing influenza.

[0052] The efficacy of the traditional Chinese medicine sachets for preventing influenza prepared in Examples 1 to 5, Comparative Example 1 and Comparative Example 2 was evaluated as follows: Existing data mining techniques have analyzed the clinical use of traditional Chinese medicine sachets in preventing and treating influenza, compiling and identifying 59 influenza sachet recipes. Studies have shown that volatile oils in traditional Chinese medicine sachets possess antibacterial, antiviral, antipyretic, analgesic, and anti-inflammatory properties. Patchouli alcohol, in particular, may block viral attachment to cells by modulating the type I helicase signaling pathway or directly binding to viral proteins. Reference "Zhu Feifei, Hu Di, Qin Yuge. A controlled study on the effect of Chinese herbal sachets in preventing colds in primary school students [J]. Bulletin of Traditional Chinese Medicine, 2023, 22(04): 61-62+68. DOI: 10.14046 / j.cnki.zyytb2002.2023.04.012." shows that Patchouli, Cyperus rotundus, Acorus gramineus, Atractylodes macrocephala, and Artemisia argyi were ground into coarse powder in a mass ratio of 1:1:1:1:1 to prepare Chinese herbal sachets for the prevention of influenza. 342 first-grade students in a primary school were selected for the observation period of wearing Chinese herbal sachets for 3 months and removing them for 2 months. It was found that the incidence of colds in primary school students who wore Chinese herbal sachets was significantly lower than that in the control group, and the clinical symptoms were also milder. The clinical effect shows that aromatic and dehumidifying Chinese herbal sachets are effective in preventing colds, have a significant effect on alleviating upper respiratory tract symptoms, and have a good effect on alleviating symptoms such as nasal congestion, runny nose, and headache. Therefore, the appearance, sensory and efficacy of the traditional Chinese medicine sachets for preventing influenza prepared by the present invention were evaluated, and the results are shown in Table 2.

[0053] Table 2 Comparison of appearance, sensory and efficacy evaluation of Chinese herbal sachets for preventing influenza Serial number Sachet weight Sachet size Portability Duration of effect Smell comfort Example 1 0.1g <![CDATA[0.04cm 3 ]]> excellent About 60 days Suitable fragrance Example 2 0.1g <![CDATA[0.04cm 3 ]]> excellent About 30 days Suitable fragrance Example 3 0.1g <![CDATA[0.04cm 3 ]]> excellent About 50 days Suitable fragrance Example 4 0.1g <![CDATA[0.04cm 3 ]]> excellent About 50 days Suitable fragrance Example 5 0.1g <![CDATA[0.04cm 3 ]]> excellent About 30 days Suitable fragrance Comparative Example 1 25g <![CDATA[100cm 3 ]]> Difference About 1 week Strong smell of Chinese medicine Comparative Example 2 25g <![CDATA[50cm 3 ]]> Difference About 1 week Strong smell of Chinese medicine As shown in Table 2, the duration of the efficacy of the traditional Chinese medicine sachet for preventing influenza prepared in Example 1 is the longest. Therefore, the physical and chemical properties of the traditional Chinese medicine sachet for preventing influenza prepared in Example 1 were characterized as follows: (1) Particle size determination.

[0054] Particle size determination: The particle size of the compound essential oil β-cyclodextrin microspheres was determined using a laser particle size analyzer. Deionized water was placed in the dispersion device of the instrument, and the compound essential oil β-cyclodextrin microspheres were added until the concentration reached the shading range. The measurement was performed at 25°C. The particle size determination results showed that the average particle size of the compound essential oil β-cyclodextrin microspheres was 709nm. Figure 4 shown.

[0055] (2) Verification of inclusion of compound essential oil β-cyclodextrin microspheres.

[0056] UV spectral scanning: Dissolve 20 mg each of the flavor-correcting compound volatile oil and β-cyclodextrin in 10 mL of anhydrous ethanol and mix to obtain solution 1. Dissolve 20 mg of the flavor-correcting compound volatile oil in 10 mL of anhydrous ethanol to obtain solution 2. Dissolve 50 mg of β-cyclodextrin in 10 mL of water to obtain solution 3. Dissolve 20 mg of the compound essential oil β-cyclodextrin microspheres in 10 mL of water to obtain solution 4. Filter all four solutions through a 0.45 μm microporous membrane, and take 200 μL of the filtrate for spectral scanning using a microplate reader within the wavelength range of 190 nm to 500 nm.

[0057] Depend on Figure 5 The scanning spectra show that the characteristic absorption peaks of the volatile oil appear at 310nm-320nm in Solutions 1 and 2, indicating that β-cyclodextrin has no absorption interference with the volatile oil in the TCM compound. The absorption peaks of Solutions 3 and 4 overlap, indicating that the volatile oil in the TCM compound has completely entered the cavity of β-cyclodextrin, obscuring the characteristic absorption peaks and proving that the volatile oil in the TCM compound has been included in the β-cyclodextrin molecules. Because the absorbance difference between water and anhydrous ethanol at 200nm-400nm is generally small, the characteristic absorption peaks overlap.

[0058] The influenza prevention Chinese medicine sachets prepared in Examples 1 to 5 were evaluated using the following test methods: The embedding efficiency of the compound essential oil β-cyclodextrin microspheres prepared in Examples 1 to 5 was compared and determined. A large embedding efficiency indicates a high utilization rate of the traditional Chinese medicine compound volatile oil.

[0059] The formula for calculating the embedding rate is: .

[0060] In the formula, E is the embedding rate, %; M 1 is the content of volatile oil of the traditional Chinese medicine compound initially added into the compound essential oil β-cyclodextrin microspheres; M 2 It is the content of volatile oil of the traditional Chinese medicine compound finally encapsulated in the compound essential oil β-cyclodextrin microspheres.

[0061] like Figure 6 As shown in the figure, when other conditions remain constant, the utilization rate of the TCM compound volatile oil shows a trend of first increasing and then decreasing with decreasing core-to-wall ratio. When the core-to-wall ratio is less than 1:4, the wall material is insufficient to completely encapsulate the TCM compound volatile oil, resulting in low utilization rate. When the core-to-wall ratio is greater than 1:4, the wall material is excessive, and the TCM compound volatile oil is insufficient to fill the β-cyclodextrin cavity, resulting in a decrease in utilization rate. When the core-to-wall ratio is 1:4, the entrapment efficiency reaches the optimal utilization rate for the TCM compound volatile oil.

[0062] The technical features of the above-mentioned embodiments can be combined arbitrarily. In order to make the description concise, not all possible combinations of the technical features in the above-mentioned embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0063] The above-described embodiments merely illustrate several implementations of the present invention. While the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that a person skilled in the art would be able to make numerous variations and improvements without departing from the spirit of the present invention, and all such variations and improvements fall within the scope of protection of the present invention.

Claims

1. A method for preparing a Chinese medicine sachet for preventing influenza, characterized in that: The following steps are involved: Weigh equal amounts of Atractylodes lancea, Patchouli, Cyperus rotundus, Acorus calamus and Artemisia argyi, mix and grind them, extract volatile oil and obtain a traditional Chinese medicine compound volatile oil; Correcting the flavor of a traditional Chinese medicine compound volatile oil to obtain the corrected flavor traditional Chinese medicine compound volatile oil; dissolving the flavor-correcting Chinese herbal compound volatile oil in anhydrous ethanol to obtain an ethanol solution of the Chinese herbal compound volatile oil; preparing a saturated aqueous solution of β-cyclodextrin; The ethanol solution of the Chinese herbal compound volatile oil was added to the saturated aqueous solution of β-cyclodextrin at a core-wall ratio of 1:2 to 1:6 for inclusion reaction, wherein the ethanol solution of the Chinese herbal compound volatile oil was the core material and the saturated aqueous solution of β-cyclodextrin was the wall material. After the inclusion reaction was completed, a Chinese herbal sachet for preventing influenza was obtained.

2. The preparation method according to claim 1, characterized in that The core-to-wall ratio is 1:3 to 1:

5.

3. The preparation method according to claim 2, characterized in that The core-to-wall ratio is 1:

4.

4. The preparation method according to claim 1, characterized in that The inclusion reaction was carried out under ultrasonication at 40° C. for 60 min.

5. The preparation method according to claim 1, characterized in that When the traditional Chinese medicine compound volatile oil is flavored, the essential oil used for flavoring includes any one or more of lavender essential oil, lemon essential oil, citrus essential oil, osmanthus essential oil, jasmine essential oil, white orchid essential oil, rose essential oil and sandalwood essential oil; The volume ratio of the flavor-correcting essential oil to the volatile oil of the traditional Chinese medicine compound is 3:

1.

6. The preparation method according to claim 1, characterized in that The volume ratio of the flavor-correcting Chinese herbal compound volatile oil to anhydrous ethanol is 1:

2.

7. The preparation method according to claim 1, characterized in that After the reaction is completed, the steps of refrigeration, filtration, washing and freeze drying are required.

8. The preparation method according to claim 7, characterized in that The refrigeration time is 24 hours.

9. The preparation method according to claim 1, characterized in that The method further comprises solidifying the influenza-preventing Chinese medicine sachet; The solidification process is as follows: jojoba oil, coconut oil and beeswax are mixed, and then mixed with the influenza-preventing traditional Chinese medicine sachet.

10. A Chinese medicinal sachet for preventing influenza prepared according to the preparation method according to any one of claims 1 to 9.

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