A bioengineered kidney construction method based on organoids and acellular scaffolds
By combining a fluid circulation perfusion culture system with decellularized kidney scaffold materials and pro-angiogenic stem cells, the problem of insufficient vascularization in the in vitro construction of kidney organoids was solved, and the complex structure and function of the kidney were reproduced, thus constructing a bioengineered kidney with glomerular filtration function.
CN120574765BActive Publication Date: 2026-06-26WENZHOU MEDICAL UNIV
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- WENZHOU MEDICAL UNIV
- Filing Date
- 2025-06-06
- Publication Date
- 2026-06-26
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Abstract
The present disclosure provides a bioengineered kidney construction method based on organoids and acellular scaffolds, which comprises first injecting pro-vascular stem cells into a kidney acellular scaffold material, and then culturing for several days through fluid circulation perfusion, and then injecting kidney organoids (KIO) into the scaffold material for perfusion culture after the kidney acellular scaffold material is recellularized. The bioengineered kidney obtained by the method of the present disclosure can be used as a graft for replacing kidney function. The present disclosure also provides a kidney organoid culture and expansion method, and the organoids obtained by the method contain not only kidney parenchymal cells such as glomerular cells and renal tubular cells, but also blood vessels and immune cells, which provides a good source of kidney parenchymal cells for the preparation of tissue-engineered kidneys.
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