A device for the sterile engineered production of mesenchymal stem cells

Abstract

The present application relates to the technical fields of cell bioengineering and regenerative medicine, and particularly relates to a mesenchymal stem cell aseptic engineering production device, which comprises a biological reaction tank, the sidewall of the biological reaction tank is provided with a liquid supplementing hole, the inner top wall of the biological reaction tank is fixedly connected with a fixing seat, the bottom of the fixing seat is provided with a driving assembly for outputting rotary power, the driving assembly comprises a gear ring, the bottom of the fixing seat is fixedly connected with a driving part, the output end of the driving part is coaxially fixedly connected with a first gear, the first gear is engaged with a plurality of second gears, the second gears are all engaged with the gear ring, and the second gears are all rotationally matched with fixing shafts, and the top ends of the fixing shafts are fixedly connected with the fixing seat. The present application drives the filter cage to rotate through the gear ring, drives the guide vanes to rotate through the rotating shaft, reduces the retention and accumulation of the cell carriers at the filter holes through the rotation of the filter cage, so that stable separation and drainage are realized in the long-period perfusion culture process, and the risk of filter unit blockage is reduced.

Description

Technical Field

[0001] This invention relates to the fields of cell bioengineering and regenerative medicine, specifically to a sterile engineered production device for mesenchymal stem cells. Background Technology

[0002] Mesenchymal stem cells (MSCs) have become a core raw material in the field of cell therapy due to their multi-lineage differentiation potential and immunomodulatory properties. To meet the massive demand for cell quantities in clinical applications, stirred bioreactors based on microcarriers combined with perfusion culture are currently the standard industrial production method. This process requires the continuous replenishment of fresh culture medium while using a cell retention device to retain cell-carrying microcarriers within the reactor and filter out metabolic waste.

[0003] According to the paper "An online method for the reduction of fouling of spin-filters for animal cell perfusion cultures" (Valdés, I. et al., 2007; PubMed PMID:17543407), "In perfusion culture systems, filter clogging is the main obstacle limiting the long-term operation of rotary filters. As the culture cycle extends, cell debris and extracellular matrix inevitably accumulate on the filter surface to form a dense biofilm, leading to increased transmembrane pressure and perfusion failure." The clogging problem identified in this paper raises two irreconcilable technological contradictions in existing engineering techniques: one is the speed matching contradiction between "high-throughput filtration" and "low-shear cell protection," meaning that high rotation speeds (>150 rpm) are usually required to generate centrifugal repulsion in order to delay biofilm formation, while the fragile suspension of MSC cells strictly requires low rotation speeds (<60 rpm). The first issue is the lack of speed (rpm), as a single motor direct connection cannot simultaneously meet the requirements; the second issue is the structural contradiction between "online cleaning" and "aseptic operation and maintenance," namely, the introduction of unclogging components such as electric scrapers will increase the dynamic sealing points on the top of the equipment, which greatly increases the risk of bacterial contamination and mechanical failure rate during long-term closed culture.

[0004] Therefore, the fundamental flaw in existing cell production devices lies in their inability to resolve the conflicting rotational speeds between filtration and suspension within a single power system, and the lack of an online anti-clogging mechanism with low sealing risk. This leads to the easy termination of long-cycle culture processes due to filter clogging. Therefore, it is necessary to propose a sterile, engineered production device for mesenchymal stem cells. Summary of the Invention

[0005] To address the aforementioned issues, this invention provides a sterile engineered production device for mesenchymal stem cells. This device uses a toothed ring to drive a filter cage to rotate, which in turn drives a guide vane to rotate. This creates a pressure difference inside and outside the filter cage, accelerating the passage of the culture medium through the filter cage and its discharge through a drain pipe. Simultaneously, the rotation of the filter cage reduces the retention and accumulation of cell carriers at the filter pores, thereby achieving stable separation and drainage during long-term perfusion culture and reducing the risk of filter unit blockage.

[0006] To achieve the above objectives, the technical solution of the present invention is as follows: A sterile engineered production device for mesenchymal stem cells includes a bioreactor, a liquid replenishment hole on the side wall of the bioreactor, a fixed base fixedly connected to the top wall of the bioreactor, a drive assembly for outputting rotational power at the bottom of the fixed base, the drive assembly including a gear ring, a drive component fixedly connected to the bottom of the fixed base, a first gear coaxially fixedly connected to the output end of the drive component, the first gear meshing with a plurality of second gears, all of the second gears meshing with the gear ring, all of the second gears rotatably engaging with a fixed shaft, and the top end of the fixed shaft being fixedly connected to the fixed base.

[0007] The bottom of the gear ring is equipped with a filter assembly for separating cell carriers and culture medium during perfusion. The filter assembly includes a filter cage coaxially fixedly connected to the bottom of the gear ring. A drain pipe is rotatably fitted to the bottom of the filter cage. The drain pipe is connected to the inside of the filter cage, and the other end of the drain pipe passes through the bottom of the bioreactor. The bottom of the first gear is equipped with a differential pressure assembly for forming a pressure difference between the inside and outside of the filter cage. The differential pressure assembly includes a rotating shaft coaxially fixedly connected to the bottom of the first gear. A guide vane is fixedly connected to the side wall of the rotating shaft.

[0008] The technical principles of the above solution are as follows: The output end of the drive unit drives the first gear to rotate. The first gear transmits power through meshing with several second gears, which in turn mesh with a gear ring to make the gear ring rotate. Culture medium is added to the bioreactor through the replenishment hole. The rotation of the gear ring drives the filter cage, which is fixedly connected to it on the same axis, to rotate. The filter cage rotates slowly during the perfusion process. At the same time, the rotation of the first gear drives the rotating shaft, which is fixedly connected to it on the same axis, to rotate. The rotating shaft drives the guide vanes to rotate, which creates directional flow inside the filter cage and a pressure difference between the inside and outside of the filter cage. Under the action of the pressure difference, the culture medium enters the filter cage through the filter holes and flows into the drain pipe. The drain pipe discharges the filtered culture medium to the outside of the bioreactor, thus completing the separation and drainage of the cell carrier and culture medium during the perfusion process.

[0009] The above approach has the following beneficial effects: 1. This invention uses a rotating shaft to drive the guide vanes to rotate, creating a pressure difference between the inside and outside of the filter cage. This makes it easier for the culture medium to pass through the filter cage and be discharged in a timely manner, reducing retention and accumulation at the filter holes. At the same time, the continuous rotation of the filter cage makes it less likely for cell carriers to adhere to the vicinity of the filter holes for a long time, further reducing adhesion and blockage, and ensuring smoother long-term operation.

[0010] 2. This invention integrates the rotation of the filter cage and the rotation of the differential pressure assembly into the same power chain through a first gear, a second gear, and a gear ring. A single drive component can simultaneously drive the filter cage and the shaft, resulting in a simpler structure, fewer drive points, and easier maintenance and assembly adjustments. Under the drive of the first gear, the gear ring drives the filter cage to rotate in the opposite direction to the shaft, creating a more significant relative motion between the inner wall of the filter cage and the guide vanes, thus enhancing the stability of the internal pressure differential of the filter cage. The second gear drives the gear ring to rotate, allowing the filter cage to achieve a lower rotation speed than the shaft, resulting in gentler rotation of the filter cage and reducing mechanical disturbance to cells and cell carriers, making it suitable for stable operation in long-term culture processes.

[0011] 3. By adjusting the rotation direction of the driving component, when the driving component reverses, it drives the rotating shaft to rotate in the opposite direction, so that the guide vanes generate positive pressure inside the filter cage and push the liquid from inside the filter cage through the filter holes to the outside, thereby backwashing the filter holes, reducing the accumulation of blockage at the filter holes and helping to restore the smooth flow of the filter channel.

[0012] Furthermore, the bottom of the second gear is provided with a cleaning component for scraping and cleaning the filter cage. The cleaning component includes a baffle, and the bottom end of the fixed shaft is fixedly connected to the baffle. Several scraping rods are fixedly connected to the bottom of the baffle, and the side wall of the scraping rods abuts against the inner side wall of the filter cage.

[0013] Beneficial effects: The baffle remains stationary with the fixed axis, and the inner wall continuously sweeps against the scraper bar when the filter cage rotates. The relative friction scrapes off the deposits adhering to the inner wall and near the filter holes in a timely manner, preventing the biofilm from accumulating and causing a decrease in flux. It is less prone to clogging during long-cycle perfusion, and the frequency of shutdown and cleaning is reduced. At the same time, the baffle is located at the top of the filter cage, which structurally isolates the internal space of the filter cage from the top gear transmission components, reducing the possibility of culture medium splashing into the gear area. This helps to reduce the risk of cross-contamination and improve the reliability of aseptic operation.

[0014] Furthermore, several bristles are fixedly connected to the side wall of the scraper bar.

[0015] Beneficial effects: By forming a flexible surface contact between the bristles and the inner wall of the filter cage, it can conform to the tiny undulations of the inner wall of the filter cage and enter the filter pores, resulting in more thorough cleaning, reducing the gradual blockage caused by repeated accumulation of local residues, and being gentler on delicate cellular systems.

[0016] Furthermore, guide ribs are fixedly connected to the outer wall of the filter cage.

[0017] Beneficial effects: The rotation of the filter cage drives the guide ribs to promote the liquid in the tank to form a stable circulation flow, making the microcarrier less prone to sedimentation and reducing local accumulation. The culture medium is renewed more evenly, the solid-liquid distribution is more stable during the perfusion process, and the load fluctuation on the filter side caused by local concentration is reduced, resulting in more stable operation.

[0018] Furthermore, the top of the bioreactor has an opening, and a cover is hinged to the opening.

[0019] Beneficial effects: The opening and cover facilitate operations such as feeding, sampling, installing or replacing filter components without disassembling the entire structure, shortening the exposure time when the cover is open, reducing the risk of contamination caused by human operation, and improving assembly and maintenance efficiency. It is suitable for on-site use in long-term continuous cultivation.

[0020] Furthermore, a sealing ring is fixedly connected at the rotatable joint between the drain pipe and the filter cage.

[0021] Beneficial effects: The sealing ring can maintain the sealing of the drainage passage, reduce leakage and backflow, and at the same time make the drainage more stable and the pressure fluctuation smaller, which is conducive to continuous drainage and cleaning maintenance during long-term irrigation.

[0022] Furthermore, the pore size of the filter cage is 20μm to 200μm.

[0023] Beneficial effects: By limiting the pore size of the filter to 20μm to 200μm, microcarriers and cell clumps can be effectively retained during perfusion and drainage, reducing the risk of carrier perforation and loss, while ensuring that the culture medium can pass through smoothly to form a usable flux; with a clear pore size window, it is easier to select and control consistency when scaling up the process, and the batch-to-batch stability is better.

[0024] Furthermore, the guide ribs are spiral ribs that extend spirally along the filter cage axis.

[0025] Beneficial effects: By setting the guide ribs as spiral ribs, the filter cage generates more continuous axial traction on the liquid when it rotates, making the circulating flow more stable and less prone to intermittent pulsation, and the microcarrier suspension state more uniform; at the same time, the spiral structure reduces local eddy dead angles, reduces local deposition, and further reduces the burden on the filter side.

[0026] Furthermore, the scraping bar is a streamlined bar with a cross-section that is circular, elliptical, or teardrop-shaped.

[0027] Beneficial effects: The scraper bar adopts a streamlined cross-section such as round, elliptical or teardrop shape, which can reduce the obstruction of the internal flow field while maintaining sufficient scraping contact, reducing the chance of material sticking and liquid accumulation; under the long-term rotation of the filter cage, the scraping is smoother and the vibration is smaller, and it is not easy to generate debris, making it suitable for long-term sterile operation.

[0028] Furthermore, the surface of the filter cage is coated with an inert coating, which is one of polytetrafluoroethylene coating, parylene coating, polyetheretherketone coating, or diamond-like carbon coating.

[0029] Beneficial effects: Coating the filter cage surface with inert coatings such as polytetrafluoroethylene, parylene, polyetheretherketone, or diamond-like carbon can reduce surface energy and decrease the tendency of protein / cell debris to adhere, making it less likely for a firm deposit layer to form around the filter pores; combined with rotation and scraping cleaning, the flux decay is slower and the continuous operation window is longer. Attached Figure Description

[0030] Figure 1 This is a schematic diagram of the filter assembly in an embodiment of the aseptic engineered production device for mesenchymal stem cells of the present invention.

[0031] Figure 2 This is a schematic diagram of the cleaning components in an embodiment of the aseptic engineered production device for mesenchymal stem cells of the present invention.

[0032] Figure 3 This is a cross-sectional view of an embodiment of the aseptic engineered production device for mesenchymal stem cells of the present invention.

[0033] Figure 4 This is a schematic diagram of the appearance of an embodiment of the aseptic engineered production device for mesenchymal stem cells of the present invention.

[0034] The reference numerals in the accompanying drawings of the instruction manual include: 1. Fixing base; 2. Driving component; 3. Rotating shaft; 4. First gear; 5. Second gear; 6. Gear ring; 7. Filter cage; 8. Baffle; 9. Drain pipe; 10. Guide vane; 11. Scraper rod; 12. Guide rib; 13. Bioreactor; 14. Cover; 15. Liquid replenishment hole; 16. Fixing shaft. Detailed Implementation

[0035] The technical solution of the present invention will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0036] In the description of this invention, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing the invention and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0037] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0038] The following detailed description illustrates the specific implementation method: Example 1:

[0039] As attached Figure 2 , Figure 3 and Figure 4 As shown: A sterile engineered production device for mesenchymal stem cells includes a bioreactor 13. The bioreactor 13 has a liquid replenishment hole 15 on its side wall. A fixed base 1 is bolted to the top wall of the bioreactor 13. The bottom of the fixed base 1 is provided with a drive assembly for outputting rotational power. The drive assembly includes a gear ring 6. A drive component 2 is bolted to the bottom of the fixed base 1. A first gear 4 is coaxially bolted to the output end of the drive component 2. The first gear 4 meshes with several second gears 5. All second gears 5 mesh with the gear ring 6. All second gears 5 are rotatably engaged with a fixed shaft 16. The top end of the fixed shaft 16 is bolted to the fixed base 1.

[0040] Specifically, after the drive unit 2 is started, it outputs rotational power and drives the first gear 4 to rotate. During the rotation, the first gear 4 continuously meshes with several second gears 5. The several second gears 5 rotate around their respective fixed shafts 16 and mesh with the gear ring 6, thereby driving the gear ring 6 to rotate. The top of the fixed shaft 16 is bolted to the fixed seat 1, so that the fixed shaft 16 maintains a stable position during operation, providing reliable rotational support for the several second gears 5, so that the gear ring 6 obtains continuous and stable rotational drive, and the rotational speed of the gear ring 6 is less than that of the first gear 4, thereby providing the appropriate power foundation for the subsequent filter components and differential pressure components.

[0041] like Figure 1 and Figure 2As shown, the bottom of the gear ring 6 is equipped with a filter assembly for separating the cell carrier from the culture medium during perfusion. The filter assembly includes a filter cage 7 coaxially bolted to the bottom of the gear ring 6. A drain pipe 9 is rotatably fitted to the bottom of the filter cage 7, and the drain pipe 9 communicates with the inside of the filter cage 7. The other end of the drain pipe 9 passes through the bottom of the bioreactor 13. The bottom of the first gear 4 is equipped with a pressure differential assembly for creating a pressure difference between the inside and outside of the filter cage 7. The pressure differential assembly includes a rotating shaft 3 coaxially bolted to the bottom of the first gear 4. A guide vane 10 is welded to the side wall of the rotating shaft 3. A guide rib 12 is welded to the outer side wall of the filter cage 7.

[0042] Specifically, as the first gear 4 rotates, it continuously meshes with several second gears 5, causing the second gears 5 to rotate around the fixed shaft 16. The second gears 5 then mesh with the gear ring 6, driving the gear ring 6 to rotate. The direction of rotation of the gear ring 6 is opposite to the direction of rotation of the first gear 4. The reverse rotation of the gear ring 6 causes the filter cage 7 to rotate in the opposite direction. At the same time, the first gear 4 drives the rotating shaft 3 to rotate in the forward direction and drives the guide vane 10 to rotate, causing the liquid inside the filter cage 7 to form a directional flow and a pressure difference between the inside and outside of the filter cage 7. Under the action of the pressure difference, the culture medium enters the filter cage 7 through the filter holes and flows into the drain pipe 9 for discharge. Because the filter cage 7 and the rotating shaft 3 rotate in opposite directions, the inner wall of the filter cage 7 forms a more obvious relative movement with the guide vane 10. The flow inside the filter cage 7 is less likely to rotate in the same direction as the rotating shaft 3, thus making the pressure difference inside the filter cage 7 more stable and the perfusion and drainage process more continuous. During the rotation of the filter cage 7, the guide ribs 12 welded to the outer wall of the filter cage 7 rotate synchronously with the filter cage 7, which exerts a continuous axial pushing effect on the culture medium in the bioreactor 13, so that the cell carrier is in a state of circulation in the bioreactor 13, thereby achieving a relatively stable solid-liquid distribution in conjunction with the perfusion and drainage process.

[0043] like Figure 2 and Figure 3 As shown, the bottom of the second gear 5 is provided with a cleaning component for scraping and cleaning the filter cage 7. The cleaning component includes a baffle 8, and the bottom end of the fixed shaft 16 is bolted to the baffle 8. Several scraping rods 11 are welded to the bottom of the baffle 8. The side wall of the scraping rod 11 abuts against the inner side wall of the filter cage 7, and several bristles are glued to the side wall of the scraping rod 11.

[0044] Specifically, the baffle 8 is bolted to the bottom of the fixed shaft 16 and remains stationary with the fixed shaft 16. The baffle 8 forms a partition structure at the upper end of the filter cage 7, creating a certain degree of isolation between the internal space of the filter cage 7 and the top gear transmission component. When the filter cage 7 rotates, the inner wall of the filter cage 7 continuously sweeps past the stationary scraping rod 11. The side wall of the scraping rod 11 abuts against the inner wall of the filter cage 7 to scrape the deposits attached to the inner wall. The bristles attached to the side wall of the scraping rod 11 further brush the inner wall of the filter cage 7 and the area around the filter holes during the scraping process, so that the deposits attached to the inner wall of the filter cage 7 and the area near the filter holes can be removed in time and carried away with the fluid, thereby maintaining the unobstructed flow of the filtration channel during long-cycle irrigation operation.

[0045] In this embodiment, the rotation of the first gear 4 and the gear ring 6 drives the filter cage 7 and the rotating shaft 3 to rotate. When the filter cage 7 rotates, it reduces the adhesion of cell carriers or cell clusters. The pressure difference component creates a pressure difference between the inside and outside of the filter cage 7 and works with the drainage pipe 9 to complete the perfusion drainage, thereby achieving the separation and export of cell carriers from culture medium. The cleaning component enables the scraper 11 and brush bristles to continuously clean the inner wall of the filter cage 7 during its rotation, thereby reducing the accumulation of deposits near the filter holes and improving operational stability during long-term perfusion culture.

[0046] Example 2:

[0047] As attached Figure 4 As shown, the difference from Embodiment 1 is that the top of the bioreactor 13 has an opening, and a cover 14 is hinged to the opening. When feeding, sampling, repairing or replacing the filter components, the operation can be completed by opening the cover 14.

[0048] A sealing ring is bonded to the rotating joint between the drain pipe 9 and the filter cage 7. This maintains a sealed joint during the rotation of the filter cage 7 and the continuous discharge of liquid, reducing the risk of leakage and making the discharge process more stable.

[0049] The filter pores on filter cage 7 have a diameter of 20μm to 200μm. In this embodiment, 80μm is selected to ensure that filter cage 7 has a stable retention capacity for cell carriers during perfusion and drainage and to ensure that culture medium passes through smoothly.

[0050] The guide rib 12 is a spiral rib that extends spirally along the axial direction of the filter cage 7, so that the guide rib 12 rotates with the filter cage 7 to continuously push the culture medium axially.

[0051] The scraping rod 11 is a streamlined rod body. The cross-section of the streamlined rod body is one of a circle, an ellipse or a teardrop shape. In this embodiment, an ellipse shape is selected so that the scraping rod 11 moves more smoothly when it comes into contact with the inner wall of the filter cage 7.

[0052] The surface of the filter cage 7 is coated with an inert coating, which is one of polytetrafluoroethylene coating, parylene coating, polyetheretherketone coating or diamond-like carbon coating. In this embodiment, parylene coating is selected to make the surface of the filter cage 7 easier to clean and to cooperate with the scraping and brushing process of the cleaning component, thereby further adapting to the requirements of long-cycle irrigation operation.

[0053] Obviously, the above embodiments are merely illustrative examples for clear explanation and are not intended to limit the implementation. Those skilled in the art will recognize that other variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations here. However, obvious variations or modifications derived therefrom are still within the scope of protection of this invention.

Claims

1. A sterile engineered production device for mesenchymal stem cells, characterized in that, The device includes a bioreactor (13), which has a liquid replenishment hole (15) on its side wall. A fixed seat (1) is fixedly connected to the top wall of the bioreactor (13). The bottom of the fixed seat (1) is provided with a drive assembly for outputting rotational power. The drive assembly includes a gear ring (6). A drive component (2) is fixedly connected to the bottom of the fixed seat (1). A first gear (4) is fixedly connected to the output end of the drive component (2) on the same axis. The first gear (4) meshes with several second gears (5). All second gears (5) mesh with the gear ring (6). All second gears (5) are rotatably engaged with a fixed shaft (16). The top of the fixed shaft (16) is fixedly connected to the fixed seat (1). The bottom of the gear ring (6) is provided with a filter assembly for separating cell carriers and culture medium during perfusion. The filter assembly includes a filter cage (7) coaxially fixedly connected to the bottom of the gear ring (6). The bottom of the filter cage (7) is rotatably fitted with a drain pipe (9). The drain pipe (9) is connected to the inside of the filter cage (7). The other end of the drain pipe (9) passes through the bottom of the bioreactor (13). The bottom of the first gear (4) is provided with a pressure differential assembly for forming a pressure difference between the inside and outside of the filter cage (7). The pressure differential assembly includes a rotating shaft (3) coaxially fixedly connected to the bottom of the first gear (4). The side wall of the rotating shaft (3) is fixedly connected with a guide vane (10).

2. The aseptic engineered production apparatus for mesenchymal stem cells according to claim 1, characterized in that, The bottom of the second gear (5) is provided with a cleaning component for scraping and cleaning the filter cage (7). The cleaning component includes a baffle (8), and the bottom end of the fixed shaft (16) is fixedly connected to the baffle (8). Several scraping rods (11) are fixedly connected to the bottom of the baffle (8), and the side wall of the scraping rod (11) abuts against the inner side wall of the filter cage (7).

3. The aseptic engineered production apparatus for mesenchymal stem cells according to claim 2, characterized in that, The scraper bar (11) has several bristles fixedly connected to its side wall.

4. The aseptic engineered production apparatus for mesenchymal stem cells according to claim 3, characterized in that, The outer wall of the filter cage (7) is fixedly connected with a flow guide rib (12).

5. The aseptic engineered production apparatus for mesenchymal stem cells according to claim 4, characterized in that, The top of the bioreactor (13) has an opening, and a cover (14) is hinged to the opening.

6. The aseptic engineered production apparatus for mesenchymal stem cells according to claim 5, characterized in that, A sealing ring is fixedly connected at the rotatable joint of the drain pipe (9) and the filter cage (7).

7. The aseptic engineered production apparatus for mesenchymal stem cells according to claim 6, characterized in that, The filter holes on the filter cage (7) have a diameter of 20μm to 200μm.

8. The aseptic engineered production apparatus for mesenchymal stem cells according to claim 7, characterized in that, The guide rib (12) is a spiral rib that extends spirally along the filter cage (7) axially.

9. The aseptic engineered production apparatus for mesenchymal stem cells according to claim 8, characterized in that, The scraping rod (11) is a streamlined rod body, and the cross-section of the streamlined rod body is one of the following: circular, elliptical or teardrop-shaped.

10. The aseptic engineered production apparatus for mesenchymal stem cells according to claim 9, characterized in that, The filter cage (7) is coated with an inert coating, which is one of polytetrafluoroethylene coating, parylene coating, polyetheretherketone coating or diamond-like carbon coating.

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