A tomato spotted wilt virus rapid detection primer, detection method, kit and application
CN120574991BActive Publication Date: 2026-08-28成都海关技术中心
Patent Information
- Application Number
- CN202510810897.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2025-06-17
- Publication Date
- 2026-08-28
- Estimated Expiration
- 2045-06-17
AI Technical Summary
Technical Problem
[0004]针对现有技术中如何实现番茄斑萎病毒快速、灵敏、准确检测的问题，本发明提供了一种番茄斑萎病毒快检引物、检测方法、试剂盒及应用
Benefits of technology
[0022]本发明所提供的番茄斑萎病毒快检引物可实现对番茄斑萎病毒快速、灵敏、准确的检测，20min即可完成检测，在直接以植物RNA为底物的前提下最低检出限可以达到1.984×10-4ng/μL，且在多病毒的情况下也具有良好的特异性。
✦ Generated by Eureka AI based on patent content.
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Figure CN120574991B_ABST
Abstract
The application discloses a tomato spotted wilt virus rapid detection primer, a detection method, a kit and application, and belongs to the technical field of virus detection. The application solves the problem of how to realize rapid, sensitive and accurate detection of the tomato spotted wilt virus. The application provides a tomato spotted wilt virus rapid detection primer, which comprises an upstream primer: SEQ ID No. 1, and a downstream primer: SEQ ID No. 2. The application also provides a detection method, a kit and application comprising the primer. The minimum detection limit of the application can reach 1.984*10 ‑4 ng / μL under the premise of directly taking plant RNA as a substrate, and the application also has good specificity in the case of multiple viruses.
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Citation Information
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