Acaricidal composition and application thereof

Abstract

The invention belongs to the technical field of pesticide mite killing, and discloses a mite killing composition and application thereof, the mite killing composition comprises an active component A and an active component B, the active ingredient A is a compound shown in a formula I: # imgabs0 #, and the active ingredient B is selected from any one of a mitochondrial respiration inhibitor acaricide, a growth inhibitor acaricide, a neurotoxin acaricide and other acaricides. The acaricidal composition disclosed by the invention is obvious in synergism, has a relatively good prevention and treatment effect on common tetranychidae pest mites of crops, and can effectively control the harm of mite pests, reduce the use dosage of pesticides and reduce the agricultural production cost.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide acaricide and discloses an acaricide composition and application thereof. Background Art

[0002] Agricultural pest mites are significant arthropods that harm crops, fruit trees, vegetables, trees, and flowers. They are characterized by their small size, wide range, rapid reproduction, short generation cycle, strong adaptability, and strong pesticide resistance. Due to the extensive use of single chemical pesticides, these pests have risen from minor pests to major pests, becoming a widespread pest group in my country. The harm caused by these pests has driven the development of acaricides, and their widespread use has led to the development of pesticide resistance in these mites, which in turn has led to new demands for pesticides.

[0003] Given the increasing severity of mite infestations, the use of pesticides to control mite infestations remains an important prevention and control measure. However, the development of pesticide resistance in mites, the limited availability of new acaricides, and the lagging technological advancements in their use have led to the overuse of these pesticides, exacerbated pollution, and the increasing difficulty of controlling mite infestations. Therefore, there is an urgent need for highly effective, safe, and environmentally friendly acaricides in agricultural production. The inventors of this application have developed an environmentally friendly acaricide with significant synergistic effects by rationally blending the compound of Formula I with other acaricides, effectively slowing the development of resistance in mites. Summary of the Invention

[0004] Based on the above, the present invention aims to provide a mite-killing composition and its use. The mite-killing composition contains a structure of formula I and another mite-killing agent, and can be used to control pest mites on crops, fruit trees, vegetables, trees, flowers, etc. The mite-killing agent has a significant synergistic effect on pest mites, is safe for the environment, can effectively slow down the development and development of drug resistance in mites, reduce the amount of pesticides used, and thus reduce agricultural production costs.

[0005] In order to achieve the above object, the present invention adopts the following technical solution: a mite-killing composition, the mite-killing composition comprises active ingredient A and active ingredient B, the active ingredient A is a compound represented by formula I: The active ingredient B is selected from any one of the following acaricides: mitochondrial respiration inhibitor acaricides, growth inhibitor acaricides, neurotoxin acaricides and other acaricides.

[0006] Furthermore, the active ingredient A is a compound represented by formula I: The active ingredient B is selected from the group consisting of mitochondrial respiration inhibitors: pyridabenz, acetofennili, vaniliprole, fipronil, fenazaquin, acetoquinol, chlorfenapyr, brofenapyr, diafenthiuron, fenthiocarb, pyraclostrobin, pyrimidifen, tebufenamide, tolfenpyrad, pyflubumide, ethoxafennili, nicofluprole, flometoquin, fenazaquin, pyrimidifen, fluazifop, chlorfenapyr, brofenapyr, pyrimidifen, fluazifop, and pyridabenz;

[0007] Growth inhibitor acaricides: clofentezine, flufenoxazine, spirotetramat, spirodiclofen, spiromesifen, spirodiclofen, tetracycline, tetracycline, etoxazole, fluxametamide, isocycloseram, cypermethrin, lufenuron, flufenoxuron, flufenoxuron, hexathiazox;

[0008] Neurotoxin acaricides: avermectin, avermectin benzoate, liuyangmycin, huaguangmycin, mibemycin, bifenazate, high-efficiency flucythrin, deltamethrin, cypermethrin, bifenthrin, flutothrin, bromofluthrin, triazotin, fenbutatin, acynonapyr;

[0009] Other acaricides: any of propargyl, flupentiofenox, amidoflumet, matrine, veratridine, azadirachtin, pyrethrin, rotenone, pine mite shark, olive shark, mivorilaner, modoflaner, tigolaner, umifoxolaner, and trifluoxetine.

[0010] Furthermore, the active ingredient B is selected from any one of fenpyroximate, spiromesifen, bifenazate, ethoxifen, diafenthiuron, abamectin, emamectin benzoate, propargyl, pyridaben, lambda-cyhalothrin, etoxazole, cyfluthrin, cypermethrin, lufenuron, fluazinam, spirocyclon, clofentezine, hexythiazox, spirotetramat or cypermethrin;

[0011] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:50 to 60:1, or any value within the above numerical range;

[0012] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:50 to 50:1, or any value within the above numerical range.

[0013] Furthermore, the active ingredient B is fenpyroximate, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 25:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:14 to 25:1, or any value within the above numerical range;

[0014] Or, the active ingredient B is spiromesifen, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 25:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:8 to 16:1, or any value within the above numerical range;

[0015] Or, the active ingredient B is bifenazate, and the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 30:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 16:1, or any value within the above numerical range;

[0016] Or, the active ingredient B is ethoxyfenociclib, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 40:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:10 to 20:1, or any value within the above numerical range;

[0017] Or, the active ingredient B is diafenthiuron, and the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 30:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:15 to 30:1, or any value within the above numerical range;

[0018] Or, the active ingredient B is avermectin, and the mass ratio of the active ingredient A to the active ingredient B is 1:15 to 50:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:8 to 40:1, or any value within the above numerical range;

[0019] Or, the active ingredient B is emamectin benzoate, and the mass ratio of the active ingredient A to the active ingredient B is 1:12 to 45:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:5 to 35:1, or any value within the above numerical range;

[0020] Alternatively, the active ingredient B is propargite, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 20:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 10:1, or any value within the above numerical range;

[0021] Or, the active ingredient B is pyridaben, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 35:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:10 to 35:1, or any value within the above numerical range;

[0022] Or, the active ingredient B is lambda-cyhalothrin, and the mass ratio of the active ingredient A to the active ingredient B is 1:32 to 38:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:12 to 24:1, or any value within the above numerical range;

[0023] Or, the active ingredient B is etoxazole, and the mass ratio of the active ingredient A to the active ingredient B is 1:24 to 44:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:24 to 30:1, or any value within the above numerical range;

[0024] Or, the active ingredient B is cyflumetofen, and the mass ratio of the active ingredient A to the active ingredient B is 1:28 to 42:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:14 to 42:1, or any value within the above numerical range;

[0025] Or, the active ingredient B is cypermethrin, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 40:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:10 to 20:1, or any value within the above numerical range;

[0026] Or, the active ingredient B is lufenuron, and the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 40:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:15 to 30:1, or any value within the above numerical range;

[0027] Or, the active ingredient B is fluazinam, and the mass ratio of the active ingredient A to the active ingredient B is 1:48 to 32:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:48 to 12:1, or any value within the above numerical range;

[0028] Or, the active ingredient B is spirodiclofen, and the mass ratio of the active ingredient A to the active ingredient B is 1:40 to 35:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 35:1, or any value within the above numerical range;

[0029] Alternatively, the active ingredient B is clofentezine, and the mass ratio of the active ingredient A to the active ingredient B is 1:24 to 24:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:24 to 16:1, or any value within the above numerical range;

[0030] Or, the active ingredient B is hexythiazox, and the mass ratio of the active ingredient A to the active ingredient B is 1:35 to 30:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:26 to 30:1, or any value within the above numerical range;

[0031] Or, the active ingredient B is spirotetramat, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 35:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 10:1, or any value within the above numerical range;

[0032] Or, the active ingredient B is cypermethrin, and the mass ratio of the active ingredient A to the active ingredient B is 1:35 to 30:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 30:1, or any value within the above numerical range;

[0033] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 30:1, or any value within the above numerical range;

[0034] Furthermore, the total weight of the acaricidal composition is 100 wt%, and the total weight of the active ingredient A and the active ingredient B accounts for 0.5% to 90% of the total weight of the acaricidal composition;

[0035] Furthermore, the total weight of the acaricidal composition is 100 wt%, and the total weight of the active ingredient A and the active ingredient B accounts for 2% to 80% of the total weight of the acaricidal composition;

[0036] Furthermore, the acaricidal composition can be prepared into an agriculturally acceptable formulation, wherein the formulation is a liquid formulation or a solid formulation, wherein the liquid formulation is selected from a soluble solution, a soluble gel, an oil, a film-spreading oil, an emulsifiable concentrate, a latex, a dispersible liquid, an ointment, an aqueous emulsion, an oil emulsion, a microemulsion, a lipid suspension, a microcapsule suspension, an oil suspension, a dispersible oil suspension, a suspoemulsion, a microcapsule suspension-suspension, a microcapsule suspension-water emulsion or a microcapsule suspension-suspoemulsion; and the solid formulation is selected from a powder, a granule, a pellet, a tablet, a strip, a wettable powder, an oil-dispersible powder, an emulsion powder, a water-dispersible granule, an emulsion granule, a water-dispersible tablet, a soluble powder, a soluble tablet or a soluble granule.

[0037] Furthermore, the liquid preparation is selected from soluble solutions, emulsifiable concentrates, aqueous emulsions, microemulsions, dispersible oil suspensions or suspension concentrates, and the solid preparation is selected from powders, granules, wettable powders or water-dispersible granules;

[0038] The present invention also discloses use of the acaricidal composition for preventing and controlling plant pests.

[0039] Furthermore, the plants are crops, fruit trees, vegetables, trees and flowers, and the harmful mites are Tetranychus cinnabarinus, Tetranychus urticae, Panonychus citri, Tetranychus truncatus and Tetranychus kanzawa;

[0040] Furthermore, the harmful mite of the Tetranychus family is Tetranychus cinnabarinus, Tetranychus urticae or Tetranychus truncatus.

[0041] Beneficial effects of the present invention:

[0042] 1) The acaricidal composition of the present invention has a significant synergistic effect on plant pests and can effectively control the damage caused by pests to plants. It can be used to control pests on crops, fruit trees, vegetables, trees, and flowers.

[0043] 2) The acaricidal composition of the present invention can preferentially delay the generation and development of drug resistance in harmful mites, is safe to their natural enemies and crops, and has little environmental residue. DETAILED DESCRIPTION

[0044] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0045] Preparation example and preparation method:

[0046] Preparation method of water-dispersible granules: According to the formula ratio, the active ingredient is added to the carrier, and the surfactant and other functional additives are added thereto, mixed, and after air flow grinding, 10-25% water is added, and then the water-dispersible granules are obtained by kneading, granulating, drying, and screening. Alternatively, the pulverized powder is sprayed with water in a boiling granulator, granulated, dried, and then screened to obtain the water-dispersible granules of the present invention.

[0047] Preparation method of water emulsion: According to the formula ratio of the preparation example, the active ingredient is dissolved in the solvent and an emulsifier is added to dissolve it into a uniform oil phase. Deionized water and antifreeze are mixed together to form a uniform water phase; under high-speed shear, the water phase is added to the oil phase to form a well-dispersed water emulsion product.

[0048] Preparation method of emulsifiable concentrate: according to the formula ratio, the measured active ingredients, solvent and cosolvent are added into a mixing kettle and stirred to dissolve them, then the emulsifier is added and the balance is supplemented with the remaining solvent, and the mixture is stirred evenly in a stirring kettle and filtered to obtain the emulsifiable concentrate of the present invention.

[0049] Preparation method of microemulsion: According to the formula ratio, the active ingredient, solvent, emulsifier, etc. are mixed to prepare the oil phase, the antifreeze solution and water are mixed to prepare the aqueous phase, the oil phase is added to the aqueous phase under stirring and stirred evenly, and shearing is continued for 10 minutes. Then, a silicone oil defoamer is added and stirred evenly to obtain small droplets of oil phase particles with a size of 0.01 to 0.1 microns, thereby preparing the microemulsion described in the present invention.

[0050] Preparation method of wettable powder: according to the formula ratio of the preparation example, the active ingredients, dispersant, wetting agent and filler are mixed, uniformly stirred in a stirring kettle, and pulverized and mixed uniformly multiple times in a jet mill to prepare the wettable powder of the composition of the present invention.

[0051] Preparation method of suspension concentrate: according to the formula ratio, the active ingredient, surfactant and other functional additives are placed in the reactor in sequence, water is added and mixed evenly, and the suspension concentrate product is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0052]

[0053]

[0054] Indoor activity test:

[0055] Example 1: Indoor toxicity determination and compound ratio screening The compound of formula I was reasonably compounded with other acaricides to determine its activity against Tetranychus truncatus

[0056] Test targets: Adults and nymphs of Tetranychus truncatus Ehara.

[0057] The test was conducted indoors according to NY / T 1154.13-2008 "Guidelines for Indoor Pesticide Bioassays - Insecticides Part 13: Leaf Disc Spray Method". Bean leaves with uniform growth were selected and suitable leaf discs were made using a hole puncher. A wet sponge was placed in a culture dish, filter paper was placed on the wet sponge, and leaf discs were placed on the filter paper, with two leaf discs per dish. Target pest mites were attached to the leaf discs, with 15 mites per disc.

[0058] The test drug was prepared into a stock solution with an organic solvent, and then 5 series of concentrations were prepared using a 0.1% Tween 80 aqueous solution in equal proportions.

[0059] The pressure of the Potter spray tower was adjusted to 1.47×10 5To determine the stable state of Pa, clean the spray head twice with acetone and then twice with distilled water. Place the petri dish in the spray tower base and spray with a spray volume of 1 mL. After the solution settles for 1 minute, remove the treated test insects and transfer them to suitable conditions for rearing and observation. Repeat each treatment four times, and set up a treatment without the agent (containing all organic solvents and emulsifiers) as a blank control. 48 hours after the agent treatment, check the test insects for mortality and record the total number of insects and the number of dead insects.

[0060] Data statistics and analysis:

[0061] Based on the survey data, calculate the mortality rate of each treatment. Calculate as follows:

[0062]

[0063] Where:

[0064] P——mortality rate, in percentage (%);

[0065] K——indicates the number of dead insects, the unit is head;

[0066] N——represents the total number of insects processed, in heads.

[0067]

[0068] Where:

[0069] P1——adjusted mortality rate, in percentage (%);

[0070] P t ——Treatment mortality rate, expressed in percentage (%);

[0071] P0 - blank control mortality rate, in percentage (%).

[0072] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to the correction mortality formula; if the control mortality rate is greater than 20%, the test needs to be repeated.

[0073] The experiment was analyzed using the DPS statistical analysis system to obtain the toxicity regression equation, correlation coefficient and LC 50 The activity of the test agent on the biological test material is evaluated by the value.

[0074] The co-toxicity coefficient (CTC value) of the mixture is calculated as follows:

[0075]

[0076] Where:

[0077] ATI - measured toxicity index of mixture;

[0078] S——LC of standard acaricide 50 , the unit is milligrams per liter (mg / L);

[0079] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0080] TTI=TI A ×P A +TI B ×P B

[0081] Where:

[0082] TTI – Theoretical Toxicity Index of Mixtures;

[0083] TI A ——Agent toxicity index;

[0084] P A ——The percentage of agent A in the mixture, in percentage (%);

[0085] TI B ——Toxicity index of agent B;

[0086] P B ——The percentage of agent B in the mixture, in percentage (%).

[0087]

[0088] Where:

[0089] CTC – Co-toxicity coefficient;

[0090] ATI - measured toxicity index of mixture;

[0091] TTI - Theoretical Toxicity Index of Mixture.

[0092] A co-toxicity coefficient (CTC) of 120 or higher indicates a synergistic effect; a co-toxicity coefficient (CTC) of 80 or lower indicates an antagonistic effect; and a co-toxicity coefficient (CTC) of 80 or lower indicates an additive effect.

[0093] Table 1 Results of indoor activity test on adult Tetranychus truncatus of mixed compound of formula I and fenpyroximate

[0094]

[0095] The test results (see Table 1) show that the mass ratio of the compound of formula I to fenpyroximate in the range of 1:20 to 25:1 exhibits a good synergistic effect on adult Tetranychus truncatus mites, among which the co-toxicity coefficient of the compound of formula I to fenpyroximate in the range of 1:14 to 25:1 is greater than 130, and the synergistic effect is significant.

[0096] Table 2 Results of indoor activity test on adult Tetranychus truncatus of mixed compound of formula I and spiromesifen

[0097]

[0098]

[0099] The test results (see Table 2) show that the compound of Formula I and spiromesifen exhibited a significant synergistic effect against adult Tetranychus truncatus mites when mixed in a ratio of 1:20 to 25:1. Specifically, when the mass ratio of the compound of Formula I to spiromesifen was within the range of 1:8 to 16:1, the co-toxicity coefficient against adult Tetranychus truncatus mites was greater than 130, indicating a significant synergistic effect.

[0100] Table 3 Indoor activity test results of the mixture of compound of formula I and bifenazate against adult Tetranychus truncatus

[0101]

[0102] The test results (see Table 3) show that the mass ratio of the compound of Formula I to bifenazate in the range of 1:25 to 30:1 exhibits a significant synergistic effect against adult Tetranychus truncatus mites. Specifically, the mass ratio of the compound of Formula I to bifenazate in the range of 1:25 to 16:1 exhibited a co-toxicity coefficient greater than 130, indicating a significant synergistic effect.

[0103] Table 4 Indoor activity test results of the mixture of compound of formula I and ethtoxafen against adult Tetranychus truncatus

[0104]

[0105]

[0106] The test results (see Table 4) show that the mass ratio of the compound of Formula I to ethtozolinone in the range of 1:20 to 40:1 exhibits a significant synergistic effect against adult Tetranychus truncatus mites. Specifically, the mass ratio of the compound of Formula I to ethtozolinone in the range of 1:10 to 20:1 exhibited a co-toxicity coefficient greater than 130, indicating a significant synergistic effect.

[0107] Table 5 Indoor activity test results of the mixture of compound of formula I and diafenthiuron against adult Tetranychus truncatus

[0108]

[0109] The test results (see Table 5) show that the mass ratio of the compound of Formula I to diafenthiuron in the range of 1:25 to 30:1 exhibits a significant synergistic effect against adult Tetranychus truncatus mites. Specifically, the mass ratio of the compound of Formula I to diafenthiuron in the range of 1:15 to 20:1 exhibited a co-toxicity coefficient greater than 130 against adult Tetranychus truncatus mites, indicating a significant synergistic effect.

[0110] Table 6 Indoor activity test results of the mixture of compound of formula I and avermectin against adult Tetranychus truncatus

[0111]

[0112] The test results (see Table 6) show that the compound of Formula I and avermectin exhibited a significant synergistic effect against adult Tetranychus truncatus mites in a mass ratio of 1:15 to 50:1. Specifically, when the mass ratio of the compound of Formula I to avermectin was within the range of 1:8 to 40:1, the co-toxicity coefficient was greater than 130, indicating a significant synergistic effect.

[0113] Table 7 Results of indoor activity test on adult Tetranychus truncatus of mixed compound of formula I and emamectin benzoate

[0114]

[0115] The test results (see Table 7) show that the compound of Formula I exhibited a significant synergistic effect against adult Tetranychus truncatus when mixed with emamectin benzoate in a mass ratio of 1:12 to 45:1. Specifically, when the mass ratio of the compound of Formula I to emamectin benzoate was within the range of 1:5 to 35:1, the co-toxicity coefficient was greater than 130, indicating a significant synergistic effect.

[0116] Table 8 Results of indoor activity test on adult Tetranychus truncatus by mixing compound of formula I with acephalitis mite

[0117]

[0118] The test results (see Table 8) show that the compound of Formula I exhibited a significant synergistic effect against adult Tetranychus truncatus when mixed with propargyl at a mass ratio of 1:30 to 20:1. Specifically, the compound of Formula I exhibited a significant synergistic effect against adult Tetranychus truncatus when mixed with propargyl at a mass ratio of 1:30 to 10:1, with a co-toxicity coefficient greater than 130.

[0119] Table 9 Indoor activity test results of the mixture of compound of formula I and pyridabenzyl against adult Tetranychus truncatus

[0120]

[0121] The test results (see Table 9) show that the compound of Formula I mixed with pyridabencil in a mass ratio of 1:20 to 35:1 exhibited a significant synergistic effect against adult Tetranychus truncatus mites. Specifically, when the mass ratio of the compound of Formula I to pyridabencil was within the range of 1:10 to 35:1, the co-toxicity coefficient was greater than 130, indicating a significant synergistic effect.

[0122] Table 10 Results of indoor activity test on adult Tetranychus truncatus of mixed compound of formula I and lambda-cyhalothrin

[0123] Test drug Virulence regression equation Correlation coefficient R <![CDATA[LC 50 (mg / L)]]> Co-toxicity coefficient Compounds of formula I y=2.7316+1.5221x 0.9954 30.9248 / Lambda-cyhalothrin y=3.9648+1.5698x 0.9998 4.5652 / 1:32 y=4.1140+1.5116x 0.9986 3.8558 121.538 1:12 y=4.2193+1.4449x 0.9990 3.4696 140.810 1:6 y=4.3274+1.3244x 0.9973 3.2201 161.429 5:1 y=3.6147+1.4532x 0.9962 8.9792 175.507 10:1 y=3.6340+1.2320x 0.9960 12.8446 157.885 24:1 y=3.2479+1.4211x 0.9994 17.0958 146.951 38:1 y=3.2961+1.2786x 0.9989 21.5074 125.244

[0124] The test results (see Table 10) show that the compound of Formula I exhibited a significant synergistic effect against adult Tetranychus truncatus mites when mixed with lambda-cyhalothrin in a mass ratio of 1:32 to 38:1. Specifically, the co-toxicity coefficient of the compound of Formula I to lambda-cyhalothrin in a mass ratio of 1:12 to 24:1 was greater than 130, indicating a significant synergistic effect.

[0125] Table 11 Results of indoor activity test on adult Tetranychus truncatus of mixed compound of formula I and etoxazole

[0126]

[0127]

[0128] The test results (see Table 11) show that the compound of Formula I mixed with etoxazole in a mass ratio of 1:24 to 44:1 exhibited a significant synergistic effect against adult Tetranychus truncatus mites. Specifically, when the mass ratio of the compound of Formula I to etoxazole was within the range of 1:24 to 30:1, the co-toxicity coefficient was greater than 130, indicating a significant synergistic effect.

[0129] Table 12 Results of indoor activity test on adult Tetranychus truncatus of mixed compound of formula I and cyflumetofen

[0130]

[0131] The test results (see Table 12) show that the compound of Formula I mixed with cyflumetofen in a mass ratio of 1:28 to 42:1 exhibited a significant synergistic effect against adult Tetranychus truncatus mites. Specifically, when the mass ratio of the compound of Formula I to cyflumetofen was within the range of 1:14 to 42:1, the co-toxicity coefficient was greater than 130, indicating a significant synergistic effect.

[0132] Table 13 Indoor activity test results of the mixture of compound of formula I and cypermethrin against adult Tetranychus truncatus

[0133]

[0134] The test results (see Table 13) show that the compound of Formula I exhibited a significant synergistic effect against adult Tetranychus truncatus when mixed with cypermethrin in a mass ratio of 1:30 to 40:1. Specifically, when the mass ratio of the compound of Formula I to cypermethrin was within the range of 1:10 to 20:1, the co-toxicity coefficient was greater than 130, indicating a significant synergistic effect.

[0135] Table 14 Results of indoor activity test on adult Tetranychus truncatus of mixed compound of formula I and lufenuron

[0136]

[0137] The test results (see Table 14) show that the compound of Formula I exhibited a significant synergistic effect against adult Tetranychus truncatus mites when mixed with lufenuron in a mass ratio of 1:25 to 40:1. Specifically, when the mass ratio of the compound of Formula I to lufenuron was within the range of 1:15 to 30:1, the co-toxicity coefficient was greater than 130, indicating a significant synergistic effect.

[0138] Table 15 Indoor activity test results of the mixture of compound of formula I and fluazinam against adult Tetranychus truncatus

[0139]

[0140] The test results (see Table 15) show that the compound of Formula I mixed with fluazinam in a mass ratio of 1:48 to 32:1 exhibited a significant synergistic effect against adult Tetranychus truncatus mites. Specifically, when the mass ratio of the compound of Formula I to fluazinam was within the range of 1:48 to 12:1, the co-toxicity coefficient was greater than 130, indicating a significant synergistic effect.

[0141] Table 16 Results of indoor activity test of the compound of formula I and spiroclofen against Tetranychus truncatus nymphs

[0142]

[0143] The test results (see Table 16) show that the compound of Formula I mixed with spirodiclofen in a mass ratio of 1:40 to 35:1 exhibited a significant synergistic effect against Tetranychus truncatus nymphs. Specifically, when the mass ratio of the compound of Formula I to spirodiclofen was within the range of 1:25 to 35:1, the co-toxicity coefficient was greater than 130, indicating a significant synergistic effect.

[0144] Table 17 Results of indoor activity test of a mixture of the compound of formula I and clofentezine against Tetranychus truncatus nymphs

[0145]

[0146] The test results (see Table 17) show that the compound of Formula I exhibited a significant synergistic effect against Tetranychus truncatus nymphs when mixed with clofentezine in a mass ratio of 1:24 to 24:1. Specifically, when the mass ratio of the compound of Formula I to clofentezine was within the range of 1:24 to 16:1, the co-toxicity coefficient was greater than 130, indicating a significant synergistic effect.

[0147] Table 18 Indoor activity test results of a mixture of the compound of formula I and hexythiazox against Tetranychus truncatus nymphs

[0148]

[0149]

[0150] The test results (see Table 18) show that the compound of Formula I exhibited a significant synergistic effect against Tetranychus truncatus nymphs when mixed with hexythiazox in a mass ratio of 1:35 to 30:1. Specifically, when the mass ratio of the compound of Formula I to hexythiazox was within the range of 1:26 to 30:1, the co-toxicity coefficient was greater than 130, indicating a significant synergistic effect.

[0151] Table 19 Results of indoor activity test on Tetranychus truncatus nymphs of a mixture of the compound of formula I and spirotetramat

[0152]

[0153] The test results (see Table 19) indicate that the compound of Formula I exhibited a significant synergistic effect against Tetranychus truncatus nymphs when mixed with spirotetramat in a mass ratio of 1:30 to 35:1. Specifically, the co-toxicity coefficient of the compound of Formula I to spirotetramat within a mass ratio of 1:26 to 30:1 was greater than 130, indicating a significant synergistic effect.

[0154] Table 20 Results of indoor activity test on Tetranychus truncatus nymphs of a mixture of the compound of formula I and cypermethrin

[0155]

[0156]

[0157] The test results (see Table 20) show that the compound of Formula I exhibited a significant synergistic effect against Tetranychus truncatus nymphs when mixed with fenpropathrin in a mass ratio of 1:35 to 30:1. Specifically, the co-toxicity coefficient of the compound of Formula I to fenpropathrin in a mass ratio of 1:26 to 30:1 was greater than 130, indicating a significant synergistic effect.

[0158] The present invention adopts the above research method, and reasonably compounding the compound of formula I with any one of profenofos, spirobudic acid, trifluanid, pyrimidine, fluazifop, butaflufen, fluazifop, sulfamethoxam, acetofen, pyrimidine, acetoquinone, chlorfenapyr (bromofenapyr), benzylpyraclostrobin, pyrimidine, pyrimidine, mitoxantrone, fipronil, tebufenpyrad, fenazaquin, bromocyclon, ethyl chlorfenapyr, fenazolidinone, triazotin, amitraz, fenbutatin, monoformamidine hydrochloride, flupentiofenox, acynonapyr, pyflubumide, and vaniliprole. The compound of formula I and the above acaricides show a synergistic effect on truncate spider mites in a mass ratio of 1:15 to 20:1.

[0159] Field efficacy test:

[0160] Example 2: Field control test of different acaricidal compositions against corn red spider mites

[0161] The experiment was conducted in corn fields in Jiaxiang County, Jining City, Shandong Province. The experimental fields were infested with a complex of spider mites, primarily Tetranychus truncatus and Tetranychus cinnabarinus. No other insecticides were used during the experiment, and all crops surrounding the experimental fields were corn. A total of 23 treatments were designed, each replicated four times, in a randomized block arrangement with plots of 30 m² each. 2 The test was conducted during the peak season of corn spider mite infestation. Mite population density was investigated before application and 3, 7, and 15 days after application. Five corn plants were surveyed in each plot, and one leaf was surveyed from each plant, from the top, middle, and bottom, for a total of 15 leaves. The corn leaves were examined with a handheld magnifying glass, and the number of active mites was recorded to calculate the control effect.

[0162] The control effect is calculated according to the following formula:

[0163]

[0164] Field control effects of each treatment on corn red spider mites:

[0165] Table 21 Field control test results of different acaricide compositions on corn red spider mites

[0166]

[0167]

[0168] During the field trial, irregular surveys were conducted and no effects of the various acaricide treatments on the corn plants themselves or their growth were found.

[0169] From the field test results of different test agents on corn red spider mites in Table 21, it can be seen that the compound of formula I mixed with any one of diafenthiuron, cypermethrin, bifenazate, emamectin benzoate, abamectin, spiromesifen, pyridabenz, ethoxaclonil, propargyl, clofentezine and fluazinam showed good control effect on corn red spider mites, and the control effect was significantly higher than that of the control single agent.

[0170] Example 3: Field control test of different acaricidal compositions on eggplant spider mites

[0171] The trial was conducted in Beihe Village, Yaozhuang Town, Jiaxing City, Zhejiang Province. The planting method was greenhouse cultivation. The trial site was flat and had medium soil fertility, which was consistent with local scientific agricultural practices.

[0172] Test crops and test targets: Eggplant, eggplant spider mites (mainly cinnabarinus and two-spotted spider mites).

[0173] Test drugs: Test drugs and control drugs are shown in Table 22.

[0174] Experimental plot arrangement: The experimental drug, control drug and blank control plots were randomly arranged, with an area of ​​10m 2 , each treatment was repeated 4 times.

[0175] Test method: The whole plant of eggplant was sprayed once using Dongfanghong DFH-16A manual sprayer at a rate of 500L / hm2 during the peak period of eggplant spider mite occurrence. 2 No other disease control agents were applied in the plots during the experiment.

[0176] Experimental investigation: The control effect was investigated on the 3rd and 14th day after the application. During the investigation, 50 leaves in each plot were surveyed to count the number of live and nymph mites.

[0177] Safety investigation: During the trial, visual inspection was used to observe the plant height, leaf color of eggplant and whether there were any adverse effects on other organisms.

[0178] The control effect is calculated according to the following formula:

[0179]

[0180]

[0181] The test results are shown in the table below:

[0182] Table 22 Field control test results of different acaricidal compositions on eggplant spider mites

[0183]

[0184] During the field trial, irregular surveys were conducted and no effects of the various acaricide treatments on the eggplant plants themselves or their growth were found.

[0185] From the field test results of different test agents on eggplant spider mites in Table 22, it can be seen that the compound of formula I mixed with any one of etoxazole, hexythiazox, cypermethrin, lufenuron, spirocyclon, cyhalothrin, cypermethrin, cyfluthrin, cyproconazole, cyfluthrin and spirotetramat showed a good control effect on eggplant spider mites.

[0186] Laboratory toxicity assays and field efficacy tests on crops such as corn and eggplant have demonstrated that the compound of Formula I described herein, when properly formulated, and the acaricidal composition described herein, exhibit excellent control efficacy against pest mites. The resulting acaricidal composition or formulation exhibits significant control efficacy, outperforming single agents in delaying the development of resistance and prolonging the duration of the effect. Furthermore, no crop damage was observed with the combined formulation during field efficacy tests, demonstrating that the resulting acaricidal composition or formulation, while enhancing synergistic acaricidal efficacy, can reduce production and use costs, and is safe for crops.

[0187] It should be understood that the above embodiments are only some of the embodiments of the present invention and are provided only for a better understanding of the embodiments of the present invention. They do not constitute all embodiments of the present invention. In actual applications, by adjusting the content of each component of the present invention and the composition of the components, various and countless embodiments can be obtained, all of which are within the scope of the present invention.

[0188] The above describes the implementation methods of the present invention. However, the present invention is not limited to the above implementation methods. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A mite-killing composition, characterized in that: The acaricidal composition comprises active ingredient A and active ingredient B, wherein the active ingredient A is a compound represented by formula I: The active ingredient B is selected from any one of the following acaricides: mitochondrial respiration inhibitor acaricides, growth inhibitor acaricides, neurotoxin acaricides and other acaricides.

2. The acaricidal composition according to claim 1, characterized in that The active ingredient A is a compound represented by formula I: The active ingredient B is selected from the group consisting of mitochondrial respiration inhibitors: pyridabenz, acetofennili, vaniliprole, fipronil, fenazaquin, acetoquinol, chlorfenapyr, brofenapyr, diafenthiuron, fenthiocarb, pyraclostrobin, pyrimidifen, tebufenamide, tolfenpyrad, pyflubumide, ethoxafennili, nicofluprole, flometoquin, fenazaquin, pyrimidifen, fluazifop, chlorfenapyr, brofenapyr, pyrimidifen, fluazifop, and pyridabenz; Growth inhibitor acaricides: clofentezine, flufenoxazine, spirotetramat, spirodiclofen, spiromesifen, spirodiclofen, tetracycline, tetracycline, etoxazole, fluxametamide, isocycloseram, cypermethrin, lufenuron, flufenoxuron, flufenoxuron, hexathiazox; Neurotoxin acaricides: avermectin, avermectin benzoate, liuyangmycin, huaguangmycin, mibemycin, bifenazate, high-efficiency cyfluthrin, high-efficiency cyhalothrin, deltamethrin, cypermethrin, bifenthrin, flutothrin, bromofluthrin, triazotin, fenbutatin, acynonapyr; Other acaricides: any of propargyl, flupentiofenox, amidoflumet, matrine, veratridine, azadirachtin, pyrethrin, rotenone, pine mite shark, olive shark, mivorilaner, modoflaner, tigolaner, umifoxolaner, and trifluoxetine.

3. The acaricidal composition according to claim 1, characterized in that The active ingredient B is selected from any one of fenpyroximate, spiromesifen, bifenazate, ethoxifen, diafenthiuron, abamectin, emamectin benzoate, propargyl, pyridaben, lambda-cyhalothrin, etoxazole, cyfluthrin, cypermethrin, lufenuron, fluazinam, spirocyclon, clofentezine, hexythiazox, spirotetramat or cypermethrin.

4. The acaricidal composition according to claim 1, characterized in that The mass ratio of the active ingredient A to the active ingredient B is 1:50 to 60:1; preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:50 to 50:

1.

5. The acaricidal composition according to claim 1, characterized in that The active ingredient B is fenpyroximate, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 25:

1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:14 to 25:1; Or, the active ingredient B is spiromesifen, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 25:

1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:8 to 16:1; Alternatively, the active ingredient B is bifenazate, and the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 30:

1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 16:1; Or, the active ingredient B is ethoxyfenociclib, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 40:

1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:10 to 20:1; Alternatively, the active ingredient B is diafenthiuron, and the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 30:

1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:15 to 30:

1. Or, the active ingredient B is avermectin, and the mass ratio of the active ingredient A to the active ingredient B is 1:15 to 50:

1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:8 to 40:1; Alternatively, the active ingredient B is emamectin benzoate, and the mass ratio of the active ingredient A to the active ingredient B is 1:12 to 45:

1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:5 to 35:

1. Alternatively, the active ingredient B is propargite, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 20:

1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 10:1; Alternatively, the active ingredient B is pyridaben, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 35:

1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:10 to 35:1; Alternatively, the active ingredient B is lambda-cyhalothrin, and the mass ratio of the active ingredient A to the active ingredient B is 1:32 to 38:

1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:12 to 24:

1. Or, the active ingredient B is etoxazole, and the mass ratio of the active ingredient A to the active ingredient B is 1:24 to 44:

1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:24 to 30:1; Or, the active ingredient B is cyflumetofen, and the mass ratio of the active ingredient A to the active ingredient B is 1:28 to 42:

1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:14 to 42:1; Or, the active ingredient B is cypermethrin, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 40:

1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:10 to 20:1; Or, the active ingredient B is lufenuron, and the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 40:

1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:15 to 30:1; Alternatively, the active ingredient B is fluazinam, and the mass ratio of the active ingredient A to the active ingredient B is 1:48 to 32:

1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:48 to 12:1; Or, the active ingredient B is spirodiclofen, and the mass ratio of the active ingredient A to the active ingredient B is 1:40 to 35:

1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 35:1; Alternatively, the active ingredient B is clofentezine, and the mass ratio of the active ingredient A to the active ingredient B is 1:24 to 24:

1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:24 to 16:

1. Alternatively, the active ingredient B is hexythiazox, and the mass ratio of the active ingredient A to the active ingredient B is 1:35 to 30:

1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:26 to 30:

1. Or, the active ingredient B is spirotetramat, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 35:

1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 10:1; Alternatively, the active ingredient B is cypermethrin, and the mass ratio of the active ingredient A to the active ingredient B is 1:35 to 30:

1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 30:

1.

6. The acaricidal composition according to claim 1, characterized in that The total weight of the acaricidal composition is 100 wt %, and the total weight of the active ingredient A and the active ingredient B accounts for 0.5% to 90%, preferably 2% to 80%, of the total weight of the acaricidal composition.

7. The acaricidal composition according to claim 1, characterized in that The acaricidal composition can be prepared into an agriculturally acceptable formulation, wherein the formulation is a liquid formulation or a solid formulation. The liquid formulation is selected from a soluble solution, a soluble gel, an oil, a film-spreading oil, an emulsifiable concentrate, a latex, a dispersible liquid, an ointment, an aqueous emulsion, an oil emulsion, a microemulsion, a lipid suspension, a microcapsule suspension, an oil suspension, a dispersible oil suspension, a suspoemulsion, a microcapsule suspension-suspension, a microcapsule suspension-water emulsion or a microcapsule suspension-suspoemulsion; the solid formulation is selected from a powder, a granule, a pellet, a tablet, a strip, a wettable powder, an oil-dispersible powder, an emulsion powder, a water-dispersible granule, an emulsion granule, a water-dispersible tablet, a soluble powder, a soluble tablet or a soluble granule.

8. The acaricidal composition according to claim 7, characterized in that The liquid preparation is selected from soluble agents, emulsifiable concentrates, aqueous emulsions, microemulsions, dispersible oil suspensions or suspensions, and the solid preparation is selected from powders, granules, wettable powders or water-dispersible granules.

9. Use of the acaricidal composition according to any one of claims 1 to 8 for controlling plant pests.

10. The use according to claim 9, characterized in that The plants are crops, fruit trees, vegetables, trees and flowers, and the pest mites are Tetranychus cinnabarinus, Tetranychus urticae, Tetranychus citri, Tetranychus truncatus and Tetranychus kanzawa; Preferably, the pest mite of the Tetranychidae family is Tetranychus cinnabarinus, Tetranychus urticae or Tetranychus truncatus.

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