Variable control device for enzyme activity test

By designing a variable control device and using a delivery pump and connecting pipes to achieve flow heating of water between the test cylinders, the problem of uneven heating in enzyme activity testing was solved, ensuring uniform heating of enzyme samples at different temperatures and improving test accuracy.

CN120591084AInactive Publication Date: 2025-09-05PEOPLES HOSPITAL OF HENAN PROV
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Patent Information

Application Number
CN202510752409.7
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-06-06
Publication Date
2025-09-05
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

Existing enzyme activity testing devices are prone to uneven heating during the heating process, which affects the test results.

Method used

By designing a variable control device, the test cylinders are connected using a delivery pump and a connecting pipe to achieve water flow heating between the test cylinders, and the temperature difference is adjusted through the heat-conducting rod and the insulation cylinder to ensure uniform heating of the enzyme sample under different temperature conditions.

Benefits of technology

Uniform heating of enzyme samples at different temperatures is achieved, which improves the accuracy and reliability of enzyme activity testing.

✦ Generated by Eureka AI based on patent content.

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Abstract

The invention relates to the technical field of enzyme activity testing, in particular to a variable control device for enzyme activity testing, which comprises a testing module, a positioning module is arranged at the bottom of the testing module, the testing module comprises a testing frame, the testing frame is fixedly connected with a top plate, and a plurality of testing cylinders are fixedly connected in the testing frame. The testing barrel is communicated with a first connecting pipe and a second connecting pipe, two conveying boxes are arranged in the testing frame, a conveying pump is arranged in each conveying box, and the water inlet end of each conveying pump is communicated with one end of the adjacent connecting pipe. According to the test device, the test cylinders are communicated with the first connecting pipe and the second connecting pipe, in the test process, a sample can be heated through water, the water in one test cylinder of one group can be conveyed into one test cylinder of the other group through the conveying pump, and due to the fact that the test cylinders of one group are communicated with one another, the water can flow in the test cylinders; therefore, the sample can be uniformly heated.
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Description

Technical Field

[0001] The invention relates to the technical field of enzyme activity testing, in particular to a variable control device for enzyme activity testing. Background Art

[0002] Enzyme activity is affected by many factors, such as temperature and pH value. Enzyme activity usually changes with temperature. Too high a temperature may cause enzyme denaturation, while too low a temperature may reduce enzyme activity. Therefore, in order to determine the optimal temperature conditions for enzyme activity, it is sometimes necessary to test enzyme activity at different temperatures. By controlling different temperature conditions, the changes in enzyme activity at different temperatures can be compared. When using existing variable control devices for enzyme activity testing, a heating mechanism is usually used to directly heat the sample. However, the heating mechanism is mostly set at the bottom of the sample. In this way, uneven heating may occur during the heating process, affecting the test effect of enzyme activity. Summary of the Invention

[0003] The object of the present invention is to provide a variable control device for enzyme activity testing to solve the problems raised in the above background technology.

[0004] To achieve the above object, the present invention provides the following technical solutions: A variable control device for enzyme activity testing comprises a test module, a positioning module is provided at the bottom of the test module, the test module comprises a test frame, the test frame is fixedly connected to a top plate, a plurality of test cylinders are fixedly connected inside the test frame, the test cylinders are connected by connecting pipe 1 and connecting pipe 2, two delivery boxes are provided inside the test frame, a delivery pump is provided inside the delivery box, the water inlet end of the delivery pump is connected to one end of an adjacent connecting pipe, and the water outlet end of the delivery pump is connected to two ends of an adjacent connecting pipe, an adjustment mechanism and a plurality of fixed pipes are provided inside the test frame, the connecting pipe 1 and the connecting pipe 2 are both connected to adjacent fixed pipes, a heat-conducting rod is fixedly connected inside the fixed pipe, the fixed pipe is slidably connected to an insulating cylinder slidably connected to the heat-conducting rod, the plurality of test cylinders are divided into two groups, a connecting pipe 1 is connected to the bottom of one fixed pipe, and a connecting pipe 2 is connected to the top of the other fixed pipe, two adjacent test cylinders in one group are connected by the fixed pipe, and the two groups of test cylinders are connected end to end by the delivery pump, the heat-conducting rod is located at the top of the fixed pipe, the insulating cylinder is located at the bottom of the fixed pipe, and a cooler is provided inside the delivery box.

[0005] Furthermore, the top of the test cylinder is slidably connected to a limit ring, a plurality of fixing rods are fixedly connected inside the limit ring, and the plurality of fixing rods are fixedly connected to a placement plate.

[0006] Furthermore, two L-shaped rods are provided inside the test frame, and the L-shaped rods are fixedly connected to the top ends of several adjacent fixed tubes. A heat conducting plate is fixedly connected inside the L-shaped rods and is fixedly connected to the top ends of several adjacent heat conducting rods. The several fixed tubes are also divided into two groups corresponding to the two groups of test tubes. The two L-shaped rods are respectively fixed on the top ends of the two groups of test tubes. The heat conducting plate is L-shaped, and the top ends of the heat conducting rods extend into the inside of the adjacent L-shaped rods.

[0007] Furthermore, the adjustment mechanism includes a fixing frame, a supporting frame, two heat-conducting rings, two heat-conducting blocks, and a heating block; The fixing frame is arranged inside the test frame, the two L-shaped rods are fixedly connected to the fixing frame, and one end of the two heat conducting plates extends into the fixing frame; The support frame is fixedly connected to the test frame and the fixed frame, a heater and a cooler are provided inside the support frame, the bottom surface of the support frame is fixed to the bottom surface of the test frame, and the top surface of the support frame is fixed to the bottom surface of the fixed frame; The two heat-conducting rings are both slidably connected to the interior of the fixed frame, and the heat-conducting rings are slidably connected to the ends of the adjacent heat-conducting plates; The two heat-conducting blocks are fixedly connected to the two heat-conducting rings respectively, and the heat-conducting blocks are slidably connected inside the fixed frame; The heating block is fixedly connected to the bottom of the fixed frame, the two heat-conducting blocks are slidably connected to the top surface of the heating block, and the bottom surface of the heating block is fixedly connected to the heater.

[0008] Furthermore, a cooling block is fixedly connected to the interior of the fixing frame, and a bottom surface of the cooling block is fixedly connected to the refrigerator.

[0009] Preferably, the fixed frame is rotatably connected to an adjusting rod, the adjusting rod is fixedly connected to two fixed blocks fixedly connected to two heat-conducting rings, a driving motor is fixedly connected inside the support frame, and the output end of the driving motor is transmission-connected to the bottom end of the adjusting rod.

[0010] Furthermore, the heat conducting ring is fixedly connected to two pads fixedly connected to adjacent fixed blocks, the pads can be filled between the heat conducting block and the fixed block, and the fixed block and the pads are both made of heat insulating material.

[0011] Furthermore, a connecting block is fixedly connected to the bottom end of the heat-insulating cylinder; The positioning module includes a bottom frame, two lifting mechanisms, and two rotating rods; The bottom frame is fixedly connected to the bottom surface of the test frame; Both lifting mechanisms are arranged inside the bottom frame; The two rotating rods are respectively arranged on the two lifting mechanisms, and the rotating rods are fixedly connected to the connecting shaft. Sliding grooves are opened at both ends of the rotating rods, and the sliding grooves are slidably connected to the sliding shafts. The connecting shaft and the two sliding shafts are rotatably connected to the adjacent connecting blocks, and the connecting shaft is located in the middle of the rotating rod.

[0012] Furthermore, the lifting mechanism includes a positioning frame, a sliding seat, and a hydraulic rod; The positioning frame is fixed inside the bottom frame; The sliding seat is slidably connected inside the positioning frame, and the connecting shaft is rotatably connected to the sliding seat; The hydraulic rod is fixedly connected to the interior of the positioning frame, and the output end of the hydraulic rod is transmission-connected to the bottom of the sliding seat.

[0013] Furthermore, the sliding seat is fixedly connected to an adjustment box rotatably connected to the connecting shaft, the connecting shaft is fixedly sleeved with a worm gear, the inside of the adjustment box is fixedly connected to a power motor, the output end of the power motor is transmission-connected to a transmission shaft rotatably connected to the adjustment box, and the transmission shaft is fixedly sleeved with a worm meshing with the worm gear.

[0014] Compared with the prior art, the present invention has the following beneficial effects: The test cylinders are connected by connecting tubes 1 and 2. A container containing an enzyme and a sample can be placed inside the test cylinder, and water is filled between the test cylinder and the container so that the water level is higher than the connecting tube 2. Several test cylinders are interconnected through connecting tubes 1 and 2. During the test process, the sample can be heated by the water. The water in one test cylinder of one group can be transferred to a test cylinder of another group by a transfer pump. Since the test cylinders in a group are interconnected, the water can flow in the test cylinders, thereby heating the sample more evenly. When the water passes through the fixed tube, it can be heated by the heat conducting rod, so that the temperature of the water in several test cylinders in a group is different. In this way, the enzyme activity can be tested under different temperature environments.

[0015] A hydraulic rod is fixed inside the positioning frame, and the hydraulic rod can be moved upward or downward inside the positioning frame through a sliding seat. The sliding seat can drive the rotating rod to move, and the rotating rod can drive the insulation tube to move, so that the insulation tube moves along the heat-conducting rod, increasing or decreasing the shielding length of the heat-conducting rod by the insulation tube. This can reduce or increase the flow distance of water along the heat-conducting rod, adjust the heating effect of the heat-conducting rod on the water, and then adjust the temperature difference of the water in the two adjacent test tubes as needed, which is conducive to the testing of enzyme activity under different temperature conditions. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the overall structure of a variable control device for enzyme activity testing according to the present invention; Figure 2This is a schematic diagram of the internal structure of the test frame in the present invention; Figure 3 This is a schematic diagram of the internal structure of the test tube in the present invention; Figure 4 This is a schematic diagram of the test tube and fixed tube structure in the present invention; Figure 5 It is a schematic diagram of the structure of the regulating mechanism in the present invention; Figure 6 This is a schematic diagram of the internal top view of the fixed frame in the present invention; Figure 7 It is a schematic diagram of the internal structure of the fixed tube in the present invention; Figure 8 It is a schematic diagram of the internal structure of the positioning module in the present invention; Figure 9 It is a schematic diagram of the rotating rod structure of the present invention; Figure 10 It is a schematic diagram of the internal structure of the lifting mechanism in the present invention.

[0017] In the figure: 100, test module; 110, test frame; 111, top plate; 120, test tube; 121, connecting tube 1; 122, connecting tube 2; 123, limiting ring; 124, fixing rod; 125, placement plate; 130, fixing tube; 131, heat-conducting rod; 132, heat-insulating tube; 133, connecting block; 140, conveying box; 150, adjustment mechanism; 151, fixing frame; 152, supporting frame; 153, heat-conducting ring; 154, heat-conducting block; 155, heating block; 156. Cooling block; 157. Adjusting rod; 158. Fixing block; 159. Spacer; 160. L-shaped rod; 161. Heat conducting plate; 200. Positioning module; 210. Bottom frame; 220. Lifting mechanism; 221. Positioning frame; 222. Sliding seat; 223. Hydraulic rod; 230. Rotating rod; 231. Connecting shaft; 232. Sliding shaft; 233. Sliding groove; 234. Worm gear; 240. Adjusting box; 241. Power motor; 242. Transmission shaft; 243. Worm. DETAILED DESCRIPTION

[0018] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0019] See also Figure 1-7In an embodiment of the present invention, a variable control device for enzyme activity testing includes a test module 100, a positioning module 200 is provided at the bottom of the test module 100, and the test module 100 includes a test frame 110, the test frame 110 is fixedly connected to a top plate 111, a plurality of test cylinders 120 are fixedly connected inside the test frame 110, the test cylinders 120 are connected by a connecting pipe 121 and a connecting pipe 2 122, two delivery boxes 140 are provided inside the test frame 110, and a delivery pump is provided inside the delivery box 140, the water inlet end of the delivery pump is connected to the adjacent end of the connecting pipe 121, and the water outlet end of the delivery pump is connected to the adjacent end of the connecting pipe 2 122, the two delivery boxes 140 are respectively provided at the two short sides of the test frame 110, and the two test cylinders 120 located on the same short side of the test frame 110 are connected through the delivery box 140, and the inside of the test frame 110 An adjustment mechanism 150 and several fixed tubes 130 are provided. Connecting tube 121 and connecting tube 2 122 are both connected to adjacent fixed tubes 130. Several test cylinders 120 are divided into two groups. Several test cylinders 120 are arranged in two rows, and several test cylinders 120 in one row form a group. A connecting tube 121 is connected to the bottom of a fixed tube 130, and a connecting tube 2 122 is connected to the top of a fixed tube 130. Two adjacent test cylinders 120 in a group are connected through the fixed tube 130, and the two groups of test cylinders 120 are connected end to end through a delivery pump. A heat-conducting rod 131 is fixedly connected inside the fixed tube 130, and a heat-insulating tube 132 slidably connected to the heat-conducting rod 131 is slidably connected to the fixed tube 130. The heat-conducting rod 131 is located at the top of the fixed tube 130, and the heat-insulating tube 132 is located at the bottom of the fixed tube 130. A cooler is provided inside the delivery box 140.

[0020] Specifically, a container containing the enzyme and sample can be placed inside the test cylinder 120, and water can be filled between the test cylinder 120 and the container so that the water level is higher than the second connecting tube 122. During the test process, two delivery pumps can be started. The delivery pump can pump water from one test cylinder 120 in one group through the first connecting tube 121, and then the delivery pump can deliver the water to one test cylinder 120 in another group through the second connecting tube 122. One delivery pump can deliver water from one test cylinder 120 in one group to another test cylinder 120, and the other delivery pump can deliver water from another test cylinder 120 in another group to one test cylinder 120. Because adjacent test cylinders 120 in a group are interconnected via the connecting tube 121, the connecting tube 122, and the fixed tube 130, water in one test cylinder 120 can flow from the connecting tube 121 into the fixed tube 130, then from the fixed tube 130 into the connecting tube 122, and finally into the adjacent test cylinder 120. This maintains the same water level within the test cylinders 120 in the group. This allows water to flow within the test cylinders 120. As the water flows within the fixed tube 130, the heat conducting rod 131 heats the water. As the water flows within the test cylinder 120, it heats the sample, resulting in relatively uniform heating of the sample. The water is heated by the heat conducting rod 131, thereby gradually increasing the temperature of the water in one group of test cylinders 120. When the water passes through the delivery box 140, the connecting tube 121 and the connecting tube 2 122 in the delivery box 140 are cooled by the cooler, thereby cooling the water in the connecting tube 121 and the connecting tube 2 122. When the water enters another group of test cylinders 120, it maintains a suitable temperature. In this way, enzyme activity can be tested under different temperature environments. Example 1

[0021] like Figure 4-7 As shown, in this embodiment, two L-shaped rods 160 are provided inside the test frame 110. The L-shaped rods 160 are fixedly connected to the top ends of several adjacent fixed tubes 130. A heat conducting sheet 161 fixedly connected to the top ends of several adjacent heat conducting rods 131 is fixedly connected inside the L-shaped rods 160. The several fixed tubes 130 are also divided into two groups corresponding to the two groups of test cylinders 120. The two L-shaped rods 160 are respectively fixed to the top ends of the two groups of test cylinders 120. The heat conducting sheet 161 is L-shaped, and the top ends of the heat conducting rods 131 extend into the interiors of the adjacent L-shaped rods 160. The adjustment mechanism 150 includes a fixing frame 151, a supporting frame 152, two heat-conducting rings 153, two heat-conducting blocks 154, and a heating block 155; The fixing frame 151 is set inside the test frame 110, and the two L-shaped rods 160 are fixedly connected to the fixing frame 151. One end of the two heat conducting plates 161 extends into the inside of the fixing frame 151. The support frame 152 is fixedly connected to the test frame 110 and the fixing frame 151. A heater and a cooler are set inside the support frame 152. The bottom surface of the support frame 152 is fixed to the bottom surface of the test frame 110, and the top surface of the support frame 152 is fixed to the bottom surface of the fixing frame 151. The fixing frame 151 is positioned and adjusted by the support frame 152. Support, the two heat-conducting rings 153 are slidably connected to the inside of the fixed frame 151, the heat-conducting rings 153 are slidably connected to the ends of the adjacent heat-conducting plates 161, the two heat-conducting blocks 154 are respectively fixedly connected to the two heat-conducting rings 153, the heat-conducting blocks 154 are slidably connected to the inside of the fixed frame 151, the heating block 155 is fixedly connected to the bottom of the fixed frame 151, the two heat-conducting blocks 154 are slidably connected to the top surface of the heating block 155, and the bottom surface of the heating block 155 is fixedly connected to the heater, and the heater can heat the heating block 155.

[0022] During specific implementation, the heater can heat the heating block 155, the heating block 155 can transfer heat to the heat-conducting block 154, the heat-conducting block 154 can transfer heat to the heat-conducting ring 153, the heat-conducting ring 153 can transfer heat to the heat-conducting sheet 161, and the heat-conducting sheet 161 can transfer heat to the heat-conducting rod 131. When water flows through the surface of the heat-conducting rod 131, the water is heated by the heat-conducting rod 131. The heat-conducting sheet 161 can be shielded and protected by the L-shaped rod 160 to minimize heat loss.

[0023] The fixing tube 130 can be optionally set in the middle of the L-shaped rod 160, and the L-shaped rod 160 and the heat conducting sheet 161 can be set to T-shape, so that heat can be transferred to the end of the heat conducting sheet 161 more quickly. In addition, multiple L-shaped rods 160 can be set between two L-shaped rods 160 as needed, so that the L-shaped rod 160 is as close to the heat conducting rod 131 as possible, so that heat can be quickly transferred to the heat conducting rod 131 through the heat conducting sheet 161.

[0024] like Figure 7-10 As shown, in this embodiment, a connection block 133 is fixedly connected to the bottom end of the heat-insulating tube 132, and the heat-insulating tube 132 is made of heat-insulating material; The positioning module 200 includes a bottom frame 210, two lifting mechanisms 220, and two rotating rods 230; The bottom frame 210 is fixedly connected to the bottom surface of the test frame 110. The two lifting mechanisms 220 are both arranged inside the bottom frame 210. The two rotating rods 230 are respectively arranged on the two lifting mechanisms 220. The two rotating rods 230 correspond to two rows of test cylinders 120, and the two rotating rods 230 correspond to two groups of fixed tubes 130. The rotating rods 230 can be supported by the lifting mechanism 220. The rotating rods 230 are fixedly connected to a connecting shaft 231. Sliding grooves 233 are provided at both ends of the rotating rod 230. The sliding grooves 233 are slidably connected to the sliding shafts 232. The connecting shaft 231 and the two sliding shafts 232 are rotatably connected to the adjacent connecting blocks 133. The connecting shaft 231 is located in the middle of the rotating rod 230 and is located between the two sliding shafts 232.

[0025] During specific implementation, the height of the corresponding rotating rod 230 can be adjusted through the lifting mechanism 220, the rotating rod 230 can drive the connecting shaft 231 to move, and the rotating rod 230 can drive the corresponding sliding shaft 232 to move through the sliding groove 233, the connecting shaft 231 and the two sliding shafts 232 can drive the connecting block 133 on the top thereof to move, so that the insulating tube 132 slides up and down inside the fixed tube 130, and the insulating tube 132 can block the heat-conducting rod 131, which can reduce or increase the flow distance of water along the heat-conducting rod 131, adjust the heating effect of the heat-conducting rod 131 on the water, and then adjust the temperature difference of the water in the two adjacent test tubes 120 as needed, which is conducive to the testing of enzyme activity under different temperature conditions.

[0026] like Figure 3 As shown, in this embodiment, the top of the test cylinder 120 is slidably connected to a limit ring 123, and a plurality of fixing rods 124 are fixedly connected inside the limit ring 123. The plurality of fixing rods 124 are fixedly connected to a placement plate 125. The test cylinder 120 can support the limit ring 123, and the limit ring 123 can support the placement plate 125 through the fixing rods 124.

[0027] In specific implementation, the container containing the enzyme and the sample can be placed on the placement plate 125 to support the container. The placement plate 125 can be replaced with a mesh plate to facilitate contact between water and the bottom of the container. Example 2

[0028] Based on the first embodiment, Figure 6As shown, in this embodiment, a cooling block 156 is fixedly connected to the interior of the fixed frame 151, and the bottom surface of the cooling block 156 is fixedly connected to the refrigerator. The top of the heating block 155 and the top of the cooling block 156 are staggered with the bottom of the heat-conducting ring 153. The fixed frame 151 is rotatably connected to an adjusting rod 157, and the adjusting rod 157 is fixedly connected to two fixed blocks 158 fixedly connected to the two heat-conducting rings 153. A driving motor is fixedly connected to the interior of the support frame 152, and the output end of the driving motor is transmission-connected to the bottom end of the adjusting rod 157. The heat-conducting ring 153 is fixedly connected to two pads 159 fixedly connected to the adjacent fixed blocks 158. The pads 159 can be filled between the heat-conducting block 154 and the fixed block 158, and the fixed block 158 and the pads 159 are both made of heat-insulating material.

[0029] During specific implementation, the driving motor can drive the adjusting rod 157 to rotate, the adjusting rod 157 can drive the two fixing blocks 158 to rotate, and the two fixing blocks 158 can drive the two heat-conducting rings 153 and the four pads 159 to rotate, thereby rotating the heat-conducting block 154, so that one heat-conducting block 154 moves toward the middle of the heating block 155, and the other heat-conducting block 154 moves toward the top of the cooling block 156, so that one heat-conducting block 154 contacts the top of the heating block 155, and the other heat-conducting block 154 contacts the top of the cooling block 156. At this time, the low temperature of the cooling block 156 can be maintained by the refrigerator, and the water When the water flows through the corresponding heat-conducting rod 131, the heat from the heat-conducting rod 131 is cooled. The heat from the heat-conducting rod 131 is transferred to the corresponding heat-conducting ring 153 via the heat-conducting sheet 161. The heat-conducting ring 153 transfers the heat to the cooling block 156 via another heat-conducting block 154. The cooling block 156 is then cooled by a refrigerator. In this way, the heat-conducting rods 131 in one set of fixed tubes 130 heat the water, while the heat-conducting rods 131 in another set of fixed tubes 130 cool the water. The temperature of the water in multiple test tubes 120 can be adjusted as needed to test enzyme activity at different temperatures. The size of the pad 159 can be increased so that the pad 159 can completely cover the top of the heating block 155 and the cooling block 156. In this way, the two heat-conducting blocks 154 are separated by the two fixing blocks 158, and the two heat-conducting rings 153 are separated. The heating block 155 and the cooling block 156 are separated by the pad 159, so as to avoid mutual influence between the heating block 155 and the cooling block 156 as much as possible.

[0030] Only the heater can be activated as needed, so that the water temperature in one group of test cylinders 120 can be gradually increased and the water temperature in another group of test cylinders 120 can be kept roughly the same, thereby testing the effects of other factors on enzyme activity at the same temperature.

[0031] like Figure 10 As shown, in this embodiment, the lifting mechanism 220 includes a positioning frame 221, a sliding seat 222, and a hydraulic rod 223; The positioning frame 221 is fixed inside the bottom frame 210, the sliding seat 222 is slidably connected inside the positioning frame 221, the connecting shaft 231 is rotatably connected to the sliding seat 222, and the rotating rod 230 is rotatably set on the top of the sliding seat 222 through the connecting shaft 231. The hydraulic rod 223 is fixedly connected inside the positioning frame 221, and the output end of the hydraulic rod 223 is transmission-connected to the bottom of the sliding seat 222. The positioning frame 221 can support the sliding seat 222 through the hydraulic rod 223, and thus support the rotating rod 230; The sliding seat 222 is fixedly connected to an adjusting box 240 that is rotatably connected to the connecting shaft 231, and the connecting shaft 231 is fixedly sleeved with a worm gear 234. The inside of the adjusting box 240 is fixedly connected to a power motor 241, and the output end of the power motor 241 is transmission-connected to a transmission shaft 242 that is rotatably connected to the adjusting box 240. The transmission shaft 242 is fixedly sleeved with a worm 243 that meshes with the worm gear 234. The worm gear 243 and the worm gear 234 can limit the transmission shaft 242 from rotating at will, thereby limiting the angle of the rotating rod 230.

[0032] During specific implementation, the hydraulic rod 223 can be used to drive the sliding seat 222 to move along the inside of the positioning frame 221, and the sliding seat 222 can drive the rotating rod 230 to move upward or downward through the connecting shaft 231. The rotating rod 230 can push the sliding shaft 232 to move through the sliding groove 233. The connecting shaft 231 and the sliding shaft 232 can drive the adjacent connecting block 133 to move, thereby causing the heat insulating tube 132 to move along the inside of the fixed tube 130, thereby changing the shielding length of the heat insulating tube 132 on the heat conducting rod 131. The transmission shaft 242 can be driven to rotate by the power motor 241, and the transmission shaft 242 can drive the worm gear 234 to rotate through the worm 243. The worm gear 234 can drive the connecting shaft 231 to rotate, and the connecting shaft 231 can be connected. The connecting shaft 231 can drive the rotating rod 230 to rotate and adjust the angle of the rotating rod 230. When the rotating rod 230 rotates, the rotating rod 230 can drive the sliding shaft 232 to move through the sliding groove 233. Since the insulation tube 132 only slides up and down along the inside of the fixed tube 130, the sliding shaft 232 can move up or down, and the sliding shaft 232 can slide in the sliding groove 233. The sliding shaft 232 drives the adjacent insulation tubes 132 to move through the connecting block 133, thereby adjusting the height of the two adjacent insulation tubes 132, adjusting the heating effect of the heat-conducting rod 131 on the water, and then adjusting the temperature difference of the water in the two adjacent test tubes 120, which is beneficial to the testing of enzyme activity under different temperature conditions.

[0033] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the invention can be embodied in other specific forms without departing from the spirit or essential characteristics of the invention. Therefore, the embodiments should be considered in all respects as illustrative and non-restrictive, and the scope of the invention is defined by the appended claims, not the foregoing description, and all variations within the meaning and range of equivalents of the claims are intended to be included therein. Any reference sign in a claim should not be construed as limiting the claim to which it relates.

[0034] In addition, it should be understood that although this specification is described in terms of implementation methods, not every implementation method contains only one independent technical solution. This narrative method of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation methods that can be understood by those skilled in the art.

Claims

1. A variable control device for enzyme activity testing, comprising a test module (100), a positioning module (200) being provided at the bottom of the test module (100), the test module (100) comprising a test frame (110), the test frame (110) being fixedly connected to a top plate (111), a plurality of test cylinders (120) being fixedly connected inside the test frame (110), the test cylinders (120) being connected to a connecting pipe 1 (121) and a connecting pipe 2 (122), characterized in that: Two conveying boxes (140) are provided inside the test frame (110), and a cooler and a conveying pump are provided inside the conveying box (140). The water inlet end of the conveying pump is connected to the end of the adjacent connecting pipe (121), and the water outlet end of the conveying pump is connected to the end of the adjacent connecting pipe (122). An adjustment mechanism (150) and a plurality of fixed pipes (130) are provided inside the test frame (110). The connecting pipe (121) and the connecting pipe (122) are both connected to the adjacent fixed pipe (130). A heat conducting rod (131) is fixedly connected inside the fixed pipe (130), and the fixed pipe (130) is slidably connected to a heat insulating tube (132) slidably connected to the heat conducting rod (131).

2. The variable control device for enzyme activity testing according to claim 1, characterized in that: The top of the test cylinder (120) is slidably connected to a limit ring (123), a plurality of fixed rods (124) are fixedly connected inside the limit ring (123), and a placement plate (125) is fixedly connected to the plurality of fixed rods (124).

3. The variable control device for enzyme activity testing according to claim 1, characterized in that: Two L-shaped rods (160) are provided inside the test frame (110), the L-shaped rods (160) are fixedly connected to the top ends of the adjacent fixed tubes (130), and a heat conducting sheet (161) is fixedly connected inside the L-shaped rods (160) and is fixedly connected to the top ends of the adjacent heat conducting rods (131).

4. The variable control device for enzyme activity testing according to claim 3, characterized in that: The regulating mechanism (150) comprises: A fixed frame (151) is disposed inside the test frame (110), and the two L-shaped rods (160) are fixedly connected to the fixed frame (151); A support frame (152) is fixedly connected to the test frame (110) and the fixed frame (151), and a heater and a cooler are provided inside the support frame (152); Two heat-conducting rings (153) are both slidably connected inside the fixed frame (151), and the heat-conducting rings (153) are slidably connected to the ends of adjacent heat-conducting sheets (161); Two heat-conducting blocks (154) are respectively fixedly connected to the two heat-conducting rings (153); The heating block (155) is fixedly connected to the bottom of the fixed frame (151), and the two heat conducting blocks (154) are both slidably connected to the top surface of the heating block (155).

5. The variable control device for enzyme activity testing according to claim 4, characterized in that: A cooling block (156) is fixedly connected inside the fixing frame (151).

6. The variable control device for enzyme activity testing according to claim 4, characterized in that: The fixed frame (151) is rotatably connected to an adjusting rod (157), and the adjusting rod (157) is fixedly connected to two fixing blocks (158) fixedly connected to the two heat-conducting rings (153). A driving motor is fixedly connected inside the support frame (152), and an output end of the driving motor is transmission-connected to the bottom end of the adjusting rod (157).

7. The variable control device for enzyme activity testing according to claim 6, characterized in that: The heat conducting ring (153) is fixedly connected to two pads (159) which are fixedly connected to adjacent fixed blocks (158).

8. The variable control device for enzyme activity testing according to any one of claims 1 to 7, characterized in that: The bottom end of the heat-insulating cylinder (132) is fixedly connected with a connecting block (133); The positioning module (200) includes: A bottom frame (210) is fixedly connected to the bottom surface of the test frame (110); Two lifting mechanisms (220) are both arranged inside the bottom frame (210); Two rotating rods (230) are respectively arranged on the two lifting mechanisms (220). The rotating rods (230) are fixedly connected to the connecting shafts (231). Sliding grooves (233) are provided at both ends of the rotating rods (230). The sliding grooves (233) are slidably connected to the sliding shafts (232). The connecting shaft (231) and the two sliding shafts (232) are rotatably connected to the adjacent connecting blocks (133).

9. The variable control device for enzyme activity testing according to claim 8, characterized in that: The lifting mechanism (220) comprises: A positioning frame (221) is fixed inside the bottom frame (210); A sliding seat (222) is slidably connected inside the positioning frame (221), and a connecting shaft (231) is rotationally connected to the sliding seat (222); The hydraulic rod (223) is fixedly connected to the interior of the positioning frame (221), and the output end of the hydraulic rod (223) is transmission-connected to the bottom of the sliding seat (222).

10. The variable control device for enzyme activity testing according to claim 9, characterized in that: The sliding seat (222) is fixedly connected to an adjusting box (240) rotatably connected to the connecting shaft (231), the connecting shaft (231) is fixedly sleeved with a worm gear (234), the adjusting box (240) is fixedly connected to a power motor (241), the output end of the power motor (241) is transmission-connected to a transmission shaft (242) rotatably connected to the adjusting box (240), and the transmission shaft (242) is fixedly sleeved with a worm (243) meshing with the worm gear (234).