Bifidobacterium longum subsp. Infantis capable of improving scalp environment, preventing hair loss and protecting hair and metagen thereof

Abstract

The invention discloses a bifidobacterium longum subsp. Infantis for improving scalp environment, preventing hair loss and protecting hair and a metagen thereof, and belongs to the technical field of washing and caring products. The bifidobacterium longum subsp. Infantis is obtained through screening, thalli and metabiotics thereof are obtained through culture, the metabiotics and the purple-leaf plum extract form a compound, and in the further prepared scalp nourishing element, the compound shows a synergistic interaction effect, specifically, the skin moisture content of a subject is remarkably improved, and the skin moisture content of the subject is remarkably reduced. The average number of hair loss of a subject is remarkably reduced, and the scalp environment is improved. The invention not only has practical value for comprehensive utilization of bifidobacterium longum subsp.infantis and metabiotics thereof, but also has important value for improving scalp environment, preventing hair loss and protecting hair.

Description

Technical Field

[0001] The present invention relates to the technical field of shampoo and conditioner products, and in particular to a strain of Bifidobacterium longum subspecies infantis for improving scalp environment, preventing hair loss and protecting hair, and a postbiotic thereof. Background Art

[0002] Maintaining healthy and lush hair is an important way to enhance one's personal temperament. In recent years, people have increasingly realized that the health of hair is closely related to the health of the scalp. Many scalp and hair loss problems can be attributed to poor scalp condition. In addition to androgenic alopecia, neurotic alopecia, and infectious alopecia, a very important cause of hair loss is the imbalance of water and oil in the scalp: As everyone generally knows, excessive sebum secretion on the head may cause seborrheic dermatitis and lead to seborrheic alopecia. However, washing the hair frequently or even every day to remove oil will have the opposite effect, causing the scalp to become too dry. This phenomenon is particularly prominent in drier areas. An overly dry scalp may not only cause the keratinocytes of the scalp to fall off and form dandruff, but also damage the hair follicles and cause hair loss.

[0003] Bifidobacterium longum subsp. infantis ( Streptococcus salivarius subsp. thermophilic ) is a gut microbe that plays a vital role in the human gut and is closely linked to intestinal health. It efficiently utilizes human milk oligosaccharides and, through the production of short-chain fatty acids (such as acetic acid), promotes the maturation of intestinal barrier function, inhibits inflammation, and enhances immune function. Because it utilizes carbohydrates from both the diet and the host's intestines, promoting metabolism, it has become an important probiotic supplement.

[0004] Purple-leaf plum ( Prunus cerasifera 'Atropurpurea' Purple-leaf plum (Prunus cerasifera) is a species of Prunus cerasifera in the Rosaceae family. Also known as red-leaf plum (Prunus cerasifera) and true red-leaf plum (Prunus serrulata), it is a deciduous shrub or small tree native to southwestern Asia. It is found in East, Central, North, Northwest, and Southwest China, with extensive cultivation in North China and its southern regions. The fruit of this sweet, fresh- or processed-fruit is rich in vitamin C, dietary fiber, and anthocyanins, offering antioxidant and digestive benefits.

[0005] Although people in various circles have a certain understanding of the efficacy of Bifidobacterium longum subsp. infantis and purple-leaf plum fruit, for a long time, the efficacy of adding Bifidobacterium longum subsp. infantis and its postbiotics in improving the scalp environment, preventing hair loss and protecting hair has remained unknown. Summary of the Invention

[0006] In view of the above-mentioned prior art, the purpose of the present invention is to provide a strain of Bifidobacterium longum subsp. infantis and its postbiotics for improving scalp environment, preventing hair loss and protecting hair.

[0007] To achieve the above object, the present invention adopts the following technical solutions: The first aspect of the present invention provides a strain of Bifidobacterium longum subsp. infantis for improving scalp environment and preventing hair loss. The Bifidobacterium longum subsp. infantis is Bifidobacterium longum subsp. infantis WZA-BI09 ( Bifidobacterium long subsp. Infant WZA-BI09), deposited in China Center for Type Culture Collection with the deposit number CCTCCNO: M 20241766.

[0008] The second aspect of the present invention provides the use of the Bifidobacterium longum subspecies infantis in the preparation of a product for improving scalp environment and preventing hair loss, wherein the amount of Bifidobacterium longum subspecies infantis added to the product is greater than 1×10 6 cfu / mL.

[0009] A third aspect of the present invention provides a postbiotic of Bifidobacterium longum subsp. infantis, wherein the postbiotic of Bifidobacterium longum subsp. infantis is prepared by the following method: The activated Bifidobacterium longum subsp. infantis is inoculated into a fermentation medium for fermentation at a temperature of 20-50° C. for 5-50 hours. The fermented product is centrifuged and the supernatant is freeze-dried to obtain the postbiotics of Bifidobacterium longum subsp. infantis.

[0010] Furthermore, the fermentation medium is composed of: 5-20 parts of beef extract powder, 5-20 parts of peptone, 10-40 parts of glucose, 2-10 parts of yeast powder, 0.1-0.5 parts of magnesium sulfate, 2-10 parts of sodium acetate, 1-5 parts of triammonium citrate, 1-5 parts of dipotassium hydrogen phosphate, 0.01-0.1 parts of manganese sulfate, 0.5-2 parts of Tween 80, 1-5 parts of L-cysteine, 800-1200 parts of distilled water, and a pH of 5-7.

[0011] The fourth aspect of the present invention provides the use of the postbiotics of Bifidobacterium longum infantis subspecies in the preparation of products for improving the scalp environment and preventing hair loss. The product includes a basic nutrient and a nutritional supplement. The basic nutrient contains a moisturizer, oil and emulsifier, and the nutritional supplement is composed of a postbiotic of Bifidobacterium longum infantis subspecies and a purple-leaf plum extract in a mass ratio of (0.5-5): (0.5-5).

[0012] Furthermore, the purple-leaf plum extract is prepared by the following method: Fresh, mature purple-leaf plums were washed, deseeded, cut into pieces, and dried in a 60°C oven. The pieces were crushed to 400 mesh and sieved to obtain purple-leaf plum powder. The purple-leaf plum powder was extracted in 75% v / v ethanol at a mass volume ratio of 1 g:3 ml at room temperature for 9 h each time, for a total of 3 extractions. The extracts obtained each time were combined, concentrated under reduced pressure at 60°C, and then dried in a 75°C oven to obtain purple-leaf plum extract.

[0013] Furthermore, the mass ratio of the basic nutrient and the nutritional supplement is (1-3) g: (97-99) g.

[0014] Furthermore, in the basic nourishing agent, the moisturizing agent, oil and emulsifier account for 5-20%, 3-12% and 0.5-2% of the total mass of the basic nourishing agent respectively, and the balance is deionized water.

[0015] Furthermore, the moisturizing agent is glycerin, the oil is squalane, and the emulsifier is glyceryl oleate.

[0016] Beneficial effects of the present invention: (1) The present invention obtains Bifidobacterium longum infantis subspecies through screening, which can improve the scalp environment and prevent hair loss.

[0017] (2) The present invention ferments Bifidobacterium longum subsp. infantis, freeze-dries the supernatant to obtain postbiotics of Bifidobacterium longum subsp. infantis, and forms a complex with a purple-leaf plum extract, namely, a Bifidobacterium longum subsp. infantis postbiotic complex.

[0018] (3) The present invention adds the Bifidobacterium longum infantis subsp. postbiotic complex to a basic nourishing agent containing a moisturizer, oil, and emulsifier to obtain a scalp nourishing agent, which is used in a subject's head skin experiment. The experimental results show that the complex group performs best. In the skin moisture test, the subject's skin moisture content increased to 48.33 after eight weeks, and the eight-week change rate of skin moisture content was 37.6%; in the average number of hair loss test, the average number of hair loss of the subject decreased to 65 after eight weeks, and the eight-week change rate of the average number of hair loss was 48.4%. This result is not only significantly superior to the control group, but also has a greater improvement compared to the postbiotic group and the purple-leaf plum group used alone, reflecting a synergistic effect. Specifically, the subject's skin moisture content increased significantly, the subject's average number of hair loss decreased significantly, and the scalp environment was improved. The present invention is not only of practical value for the comprehensive utilization of Bifidobacterium longum infantis subsp. and its postbiotics, but also of important value for improving the scalp environment and preventing hair loss. DETAILED DESCRIPTION

[0019] It should be noted that the following detailed descriptions are illustrative and intended to provide further explanation of the present application. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which the present application belongs.

[0020] In order to enable those skilled in the art to more clearly understand the technical solution of the present application, the technical solution of the present application will be described in detail below with reference to specific embodiments.

[0021] The experimental materials used in the examples of the present invention but not specifically described are all conventional experimental materials in the art and can be purchased through commercial channels.

[0022] The deposit information of Bifidobacterium longum subspecies infantis used in the present invention is as follows: Chinese name: Bifidobacterium longum subsp. infantis WZA-BI09; Latin name: Bifidobacterium longum subsp. Infant WZA-BI09; Deposit number: CCTCC NO:M 20241766; Deposit date: August 9, 2024; Depository: China Center for Type Culture Collection (CCTCC); Deposit: Wuhan University, Wuhan, China.

[0023] Example 1: Screening of Bifidobacterium longum subsp. infantis 1. Isolation and initial screening of strains: A 10-g fecal sample was collected from the infants. 1 g of each sample was placed in a 50-mL centrifuge tube containing 9 mL of sterile saline. The mixture was shaken for 15 minutes to thoroughly mix and allowed to rest for 25 seconds. 2 mL of the suspension was aspirated from each sample and placed in an 80°C water bath for 10 minutes. 1 mL of each suspension was then diluted 10-fold. The appropriate suspension was then spread on MRS agar medium (prepared by hand) containing 0.3% CaCO₃ and incubated in a 37°C incubator for 48 hours. Single colonies exhibiting bifidobacterium colony morphology were selected and examined microscopically using Gram staining. Gram-positive bacteria were selected. Among these Gram-positive bacteria, strains that retained bifidobacterium colony morphology after multiple streaking on solid screening medium were designated as target strains and stored with serial numbers.

[0024] The solid screening medium used was prepared as follows: 50 mL of filtered horse serum, 0.05% L-cysteine ​​hydrochloride, and 0.05 mg / mL mupirocin were added per liter to MRS medium, wherein L-cysteine ​​hydrochloride was sterilized by filtration through a sterile filter membrane. After high-pressure sterilization, mupirocin was added when the medium was cooled to 40°C-50°C.

[0025] 2. Strain identification: The strain was sent to Henan Industrial Microbial Engineering Technology Research Center for 16S rDNA gene sequence identification, the sequence is shown in SEQ ID NO: 1, and the identification results showed that the strain belonged to Bifidobacterium longum subsp. infantis ( Bifidobacterium longum subsp. Infant ). The strain WZA-BI09 was biologically deposited.

[0026] Example 2: Preparation of Postbiotics of Bifidobacterium longum subsp. infantis The preparation method is as follows: (1) Activate the Bifidobacterium longum subsp. infantis preserved in Example 1 to obtain an activated bacterial solution of Bifidobacterium longum subsp. infantis. The activation temperature is 37° C. and the activation time is 24 h.

[0027] (2) The activated bacterial liquid of Bifidobacterium longum subsp. infantis obtained in step (1) is inoculated into a fermentation medium at an inoculum amount of 3 wt% (g / g) of the fermentation medium to obtain a fermentation liquid of Bifidobacterium longum subsp. infantis. The fermentation temperature is 37° C., the fermentation time is 48 h, and the anaerobic fermentation medium is composed of the following: beef extract powder: 10.0 g, peptone: 10.0 g, glucose: 20.0 g, yeast powder: 4.0 g, magnesium sulfate: 0.2 g, sodium acetate: 5 g, triammonium citrate: 2.0 g, dipotassium hydrogen phosphate: 2.0 g, manganese sulfate: 0.05 g, Tween 80: 1.0 g, L-cysteine: 2 g, and distilled water is added to 1 L, with a pH of 6.2.

[0028] (3) taking the fermentation liquid of Bifidobacterium longum subsp. infantis obtained in step (2), centrifuging it at 30° C., and freeze-drying the supernatant to obtain the postbiotics of Bifidobacterium longum subsp. infantis.

[0029] The content of Bifidobacterium longum subspecies infantis in the sediment was determined by spectrophotometry. The viable bacterial count was 5×10 8 The solution was aliquoted and freeze-dried at a concentration of cfu / ml, and then reconstituted with water to the concentration to obtain a solution of Bifidobacterium longum subsp. infantis, which was then stored in a -18°C refrigerator.

[0030] Example 3: Preparation of Bifidobacterium longum subsp. infantis postbiotic complex Preparation of Purple Leaf Plum Extract Fresh, ripe purple-leaf plum fruits were peeled, stemmed, and pitted, cut into pieces, and dried in a 60°C oven. The pieces were crushed to 400 mesh and sieved to obtain purple-leaf plum powder. The purple-leaf plum powder was extracted in 75% v / v ethanol at a mass volume ratio of 1 g:3 ml at room temperature for 9 h each time, for a total of 3 extractions. The extracts obtained each time were combined, concentrated under reduced pressure at 60°C, and then dried in a 75°C oven to obtain the purple-leaf plum extract.

[0031] Preparation of complexes The postbiotics of Bifidobacterium longum subsp. infantis prepared in Example 2 and the purple-leaf plum extract prepared in Example 3 were mixed in a mass ratio of 1:1 to obtain a Bifidobacterium longum subsp. infantis postbiotic complex.

[0032] Example 4: Preparation of scalp nourishing agent Basic Nourishing Factor The basic nourishing agent contains 10% moisturizer, 6% oil, 1% emulsifier, and the balance is deionized water, accounting for the total mass percentage (g / g). Among them, the moisturizer is glycerin, the oil is squalane, and the emulsifier is glycerol oleate.

[0033] Scalp Nourishing Serum The nutritional supplement was added to the basic nourishing agent at a mass ratio of 2 g:98 g to obtain a scalp nourishing agent. The nutritional supplement used was the Bifidobacterium longum subsp. infantis postbiotic complex prepared in Example 4.

[0034] Example 5: Preparation of scalp nourishing agent Basic Nourishing Factor The basic nourishing agent contains 10% moisturizer, 6% oil, 1% emulsifier, and the balance is deionized water, accounting for the total mass percentage (g / g). Among them, the moisturizer is glycerin, the oil is squalane, and the emulsifier is glycerol oleate.

[0035] Scalp Nourishing Serum The nutritional supplement was added to the basic nourishing agent at a mass ratio of 1 g:99 g to obtain a scalp nourishing agent. The nutritional supplement used was the Bifidobacterium longum subsp. infantis postbiotic complex prepared in Example 4.

[0036] Example 6: Preparation of scalp nourishing agent Basic Nourishing Factor The basic nourishing agent contains 10% moisturizer, 6% oil, 1% emulsifier, and the balance is deionized water, accounting for the total mass percentage (g / g). Among them, the moisturizer is glycerin, the oil is squalane, and the emulsifier is glycerol oleate.

[0037] Scalp Nourishing Serum The nutritional supplement was added to the basic nourishing agent at a mass ratio of 3 g:97 g to obtain a scalp nourishing agent. The nutritional supplement used was the Bifidobacterium longum subsp. infantis postbiotic complex prepared in Example 4.

[0038] Comparative Example 1: Preparation of Scalp Nourishing Agent The difference between Comparative Example 1 and Example 4 is that the nutritional supplement used is the postbiotic of Bifidobacterium longum subsp. infantis prepared in Example 2.

[0039] Basic Nourishing Factor The basic nourishing agent contains 10% moisturizer, 6% oil, 1% emulsifier, and the balance is deionized water, accounting for the total mass percentage (g / g). Among them, the moisturizer is glycerin, the oil is squalane, and the emulsifier is glycerol oleate.

[0040] Scalp Nourishing Serum Add nutritional supplements to the basic nourisher in a mass ratio of 2g:98g to obtain scalp nourisher.

[0041] Comparative Example 2: Preparation of Scalp Nourishing Agent The difference between Comparative Example 2 and Example 4 is that the nutritional supplement used is the purple-leaf plum extract prepared in Example 3.

[0042] Basic Nourishing Factor The basic nourishing agent contains 10% moisturizer, 6% oil, 1% emulsifier, and the balance is deionized water, accounting for the total mass percentage (g / g). Among them, the moisturizer is glycerin, the oil is squalane, and the emulsifier is glycerol oleate.

[0043] Scalp Nourishing Serum Add nutritional supplements to the basic nourisher in a mass ratio of 2g:98g to obtain scalp nourisher.

[0044] Comparative Example 3: Preparation of Scalp Nourishing Agent The difference between Comparative Example 3 and Example 4 is that the nutritional supplement used is the Bifidobacterium longum subsp. infantis bacterial solution prepared in Example 2.

[0045] Basic Nourishing Factor The basic nourishing agent contains 10% moisturizer, 6% oil, 1% emulsifier, and the balance is deionized water, accounting for the total mass percentage (g / g). Among them, the moisturizer is glycerin, the oil is squalane, and the emulsifier is glycerol oleate.

[0046] Scalp Nourishing Serum Add nutritional supplements to the basic nourisher in a mass ratio of 2g:98g to obtain scalp nourisher.

[0047] Test example A total of 40 volunteers aged 25-55 years were selected as subjects and randomly divided into 5 groups, with 8 people in each group, including 4 males and 4 females.

[0048] Experimental Grouping: The groups were named postbiotic group, purple-leaf plum group, microbial group, complex group, and control group. The test samples used in each group were as follows: the postbiotic group used the scalp nourishing agent prepared in Comparative Example 1; the purple-leaf plum group used the scalp nourishing agent prepared in Comparative Example 2; the microbial group used the scalp nourishing agent prepared in Comparative Example 3; the complex group used the scalp nourishing agent prepared in Example 4; and the control group used the basal nourishing agent.

[0049] Instructions for use: Apply 5 mL of the designated scalp conditioner to the scalp once nightly and gently massage for 3-5 minutes until absorbed. Note: During the trial, participants were to shampoo as per their daily routine, at least three times per week, using only the provided shampoo (which contained no active ingredients). During the experiment, participants were not to use any other hair products, medications, or supplements that could affect the results, or engage in any other activities that could interfere with the results. Participants were not to shampoo within 48 hours before each statistical test and were not to comb their hair on the day of the test.

[0050] The specific statistical test methods are as follows: Before each test, the subjects sat quietly for 30 minutes in a room with a temperature of 20°C and a relative humidity of 50%.

[0051] To test the average number of hairs shed, technicians used a comb of the same specifications (moderate tooth density, 1mm tooth spacing, 2.0-3.0 cm tooth length, and 10 cm comb length) and combed the hairs from front left to back left and then from front right to back right 60 times at a constant speed (30 times each). The shed hairs were collected and counted.

[0052] For skin hydration testing, the Corneometer CM825 skin moisture meter was used to measure the scalp stratum corneum moisture content in a fixed test area on the top of the head before and after application (30 minutes and 1 hour). Three measurements were taken and the average was used to calculate the moisture content. The same area was tested each subsequent time. Subjects continued using the product for eight weeks, with skin tests performed at the fourth and eighth weeks. The skin hydration test results are shown in Table 1.

[0053] Table 1 Skin moisture content As shown in Table 1, compared with the control group, the skin moisture content of the subjects in each experimental group increased significantly after eight weeks. Among them, the complex group performed best. In the complex group, the skin moisture content of the subjects was 48.33 at eight weeks, and the eight-week change rate of skin moisture content was 37.6%. It can be seen that by adding the Bifidobacterium longum subsp. infantis postbiotic complex prepared in Example 4 to the basic nourishment, the hydrating and moisturizing effect of the scalp nourishment was improved, and it was more helpful to improve the moisturizing performance of the scalp. After treatment with the complex group, the skin moisture content of the subjects increased significantly, and the scalp environment was improved.

[0054] The results of the hair loss average test are shown in Table 2.

[0055] Table 2 Average number of hair loss (roots) As shown in Table 2, after eight weeks, the average number of hair loss in all experimental groups decreased significantly compared to the control group. The complex group performed best, with an average of 65 hairs lost at eight weeks, representing an eight-week change rate of 48.4%. This indicates that the addition of the Bifidobacterium longum subsp. infantis postbiotic complex prepared in Example 4 to the base nutrient enhances the anti-hair loss and care effects of the scalp nutrient. Treatment with the complex group improved the scalp environment.

[0056] In summary, the complex group performed the best. In the skin hydration test, the subjects' skin hydration increased to 48.33 after eight weeks, with a 37.6% change rate. In the average number of hair loss test, the average number of hair loss decreased to 65 after eight weeks, with a 48.4% change rate. This result not only significantly outperformed the control group but also significantly improved compared to the postbiotic and purple-leaf plum groups used alone, demonstrating a synergistic effect. Specifically, the subjects' skin hydration increased significantly, the average number of hair loss decreased significantly, and the scalp environment improved.

[0057] The above description is merely a preferred embodiment of the present application and is not intended to limit the present application. Various modifications and variations are possible for those skilled in the art. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present application shall be included within the scope of protection of the present application.

Claims

1. A strain of Bifidobacterium longum subspecies infantis for improving scalp environment, preventing hair loss and protecting hair, characterized by: The Bifidobacterium longum subspecies infantis is Bifidobacterium longum subspecies infantis WZA-BI09 ( Bifidobacterium longum subsp. lnfantis WZA-BI09), deposited in China Center for Type Culture Collection with the deposit number CCTCC NO: M 20241766.

2. The use of Bifidobacterium longum subspecies infantis according to claim 1 in the preparation of products for improving scalp environment and preventing hair loss, characterized in that: The amount of Bifidobacterium longum subspecies infantis added to the product is greater than 1×10 6 cfu / mL.

3. A postbiotic of Bifidobacterium longum subsp. infantis, characterized in that The postbiotics of Bifidobacterium longum subspecies infantis are prepared by the following method: The Bifidobacterium longum subsp. infantis described in claim 1 is activated and inoculated into a fermentation medium for fermentation at a temperature of 20-50° C. for 5-50 hours. The fermented product is centrifuged and the supernatant is freeze-dried to obtain the postbiotics of Bifidobacterium longum subsp. infantis.

4. The postbiotic of Bifidobacterium longum subsp. infantis according to claim 3, characterized in that The fermentation medium is composed of: 5-20 parts of beef extract powder, 5-20 parts of peptone, 10-40 parts of glucose, 2-10 parts of yeast powder, 0.1-0.5 parts of magnesium sulfate, 2-10 parts of sodium acetate, 1-5 parts of triammonium citrate, 1-5 parts of dipotassium hydrogen phosphate, 0.01-0.1 parts of manganese sulfate, 0.5-2 parts of Tween 80, 1-5 parts of L-cysteine, 800-1200 parts of distilled water, and a pH of 5-7.

5. Use of the postbiotics of Bifidobacterium longum subspecies infantis according to claim 3 or 4 in the preparation of products for improving scalp environment and preventing hair loss, characterized in that: The product includes a basic nutrient and a nutritional supplement. The basic nutrient contains a moisturizer, oil and an emulsifier. The nutritional supplement is prepared by mixing the postbiotics of Bifidobacterium longum infantis subspecies and purple-leaf plum extract in a mass ratio of (0.5-5):(0.5-5).

6. The use of the postbiotic of Bifidobacterium longum subspecies infantis according to claim 5 in the preparation of a product for improving scalp environment and preventing hair loss, characterized in that: The purple-leaf plum extract is prepared by the following method: Fresh, mature purple-leaf plums were washed, deseeded, cut into pieces, and dried in a 60°C oven. The pieces were crushed to 400 mesh and sieved to obtain purple-leaf plum powder. The purple-leaf plum powder was extracted in 75% v / v ethanol at a mass volume ratio of 1 g:3 ml at room temperature for 9 h each time, for a total of 3 extractions. The extracts obtained each time were combined, concentrated under reduced pressure at 60°C, and then dried in a 75°C oven to obtain purple-leaf plum extract.

7. The use of the postbiotic of Bifidobacterium longum subspecies infantis according to claim 5 in the preparation of a product for improving scalp environment and preventing hair loss, wherein: The mass ratio of basic nutrients and nutritional supplements is (1-3) g: (97-99) g.

8. The use of the postbiotics of Bifidobacterium longum subspecies infantis according to claim 7 in the preparation of products for improving scalp environment and preventing hair loss, characterized in that: In the basic nourishing agent, the moisturizing agent, the oil and the emulsifier account for 5-20%, 3-12% and 0.5-2% of the total mass of the basic nourishing agent respectively, and the balance is deionized water.

9. The use of the postbiotic of Bifidobacterium longum subspecies infantis according to claim 8 in the preparation of a product for improving scalp environment and preventing hair loss, wherein: The moisturizer is glycerin, the oil is squalane, and the emulsifier is glyceryl oleate.

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