Campylobacter jejuni detector and method

Abstract

The invention relates to the field of bacterial detection, and discloses a campylobacter jejuni detector and method, the detector comprises an automatic horizontal adjusting mechanism, an automatic clamping assembly, and a buffer cushion elasticity adjusting unit based on sample weight induction; the automatic horizontal adjusting mechanism ensures that the bearing platform is always horizontal through a horizontal sensor, a control unit and a telescopic automatic supporting column, the accuracy of a detected image is guaranteed, and the detection efficiency and stability are improved; the automatic clamping assembly accurately clamps the sample in the center of the bearing platform through the driving assembly, and the integrity of the sample is protected through cooperation with an elastic piece and a buffer pad. The induction correction assembly and the elastic adjusting unit automatically adjust the elasticity modulus of the buffer pad according to the sample weight, the adaptability to samples with different weights is improved, and the service life of an instrument is prolonged. The detector integrally improves the accuracy, comprehensiveness and reliability of detection, and has a wide application prospect and a remarkable practical value.

Description

Technical Field

[0001] The present invention relates to the field of bacteria detection, and in particular to a Campylobacter jejuni detection instrument and method. Background Art

[0002] Campylobacter jejuni is a common foodborne pathogen that is comma-shaped, S-shaped, or spiral-shaped. It is microaerophilic and thrives at 42°C. It is widely distributed in nature and often enters the human body through contaminated food and water, causing intestinal illnesses such as diarrhea, abdominal pain, and fever. In severe cases, it can even lead to complications, posing a significant threat to public health.

[0003] Detecting Campylobacter jejuni is extremely important because the foodborne diseases it causes not only affect individual health but may also spread among the population. Especially in scenarios such as the catering industry, food processing companies, and water source monitoring, timely and accurate detection of Campylobacter jejuni can effectively prevent the outbreak and spread of related diseases and ensure food safety and public health.

[0004] Most existing C. jejuni detection instruments utilize high-pixel CCD cameras to capture images of plates containing C. jejuni. The captured images are then transmitted to a computer system for processing and analysis via specialized software, enabling accurate identification and counting of C. jejuni colonies. However, several drawbacks remain. For example, when testers place plates or petri dishes containing C. jejuni, they often tilt the placement angle, causing perspective distortion in the images captured by the CCD camera and interfering with the analysis software's ability to accurately identify and count the colonies. Imprecise placement can easily place some colonies in blind spots, leading to missed detection. Repeated adjustments to the placement position waste time, increase the risk of plate contamination, and significantly extend the testing cycle, severely impacting efficiency. This makes it impossible to meet the timeliness and accuracy requirements of industries like catering and food processing. Summary of the Invention

[0005] The object of the present invention is to provide a Campylobacter jejuni detector and method to solve the technical problem caused by improper placement of plates or culture dishes cultured with Campylobacter jejuni by detection personnel.

[0006] The purpose of the present invention can be achieved through the following technical solutions:

[0007] A Campylobacter jejuni detector includes a detection box, a detection camera is provided on the top of the detection box, a carrying platform for placing a flat plate or a culture dish is provided in the bottom area of ​​the detection box opposite the detection camera, an automatic leveling mechanism is provided below the carrying platform, the automatic leveling mechanism is used to keep the carrying platform in a horizontal state at all times; an automatic clamping component is also provided on the carrying platform, the automatic clamping component is used to keep the flat plate or culture dish in the center position of the carrying platform.

[0008] As a further technical solution, the automatic level adjustment mechanism includes an automatic support column; at least three independently retractable automatic support columns are connected to the bottom of the carrying platform, and the automatic support columns are controlled by a control unit; a level sensor is provided on the carrying platform, and the level sensor is electrically connected to the control unit. The level sensor detects the horizontal state of the carrying platform in real time and transmits the signal to the control unit. The control unit controls the action of the No. 1 drive motor according to the received signal, and adjusts the retractable length of the automatic support column to keep the carrying platform always in a horizontal state.

[0009] As a further technical solution, the automatic telescopic column is an electric telescopic rod, which includes an outer sleeve, an inner telescopic rod, a No. 1 drive motor, and a transmission screw arranged between the outer sleeve and the inner telescopic rod. The output shaft of the No. 1 drive motor is connected to the transmission screw, and the No. 1 drive motor drives the transmission screw to rotate through forward and reverse rotation to realize the extension and retraction of the inner telescopic rod in the outer sleeve; the connection part between the load-bearing platform and the support column is provided with a universal ball joint to adapt to the angle change of the load-bearing platform during the adjustment process.

[0010] As a further technical solution, the automatic clamping assembly includes:

[0011] A limiting groove is provided on the carrying platform;

[0012] There are two limit sliders, which are symmetrically distributed and slidably connected in the limit slot;

[0013] The clamping member is provided in a one-to-one correspondence with the limit slider and is provided in a concave structure;

[0014] The elastic member is arranged between the limiting slider and the side wall of the limiting groove.

[0015] As a further technical solution, the automatic clamping assembly further includes a driving assembly, which is arranged on the carrying platform and includes:

[0016] A gear is rotatably arranged at the middle position of the bottom surface of the carrying platform;

[0017] Two racks are provided, symmetrically distributed on both sides of the limiting slide and meshing with the gear, and each rack is fixed with a corresponding limiting slider;

[0018] The second driving motor is arranged on the bearing platform and is connected to the gear transmission.

[0019] As a further technical solution, a buffer pad is installed on the side of the clamping member that contacts the flat plate or culture dish, and the elastic adjustment of the buffer pad based on the weight sensing of the flat plate or culture dish is achieved through an induction correction component.

[0020] As a further technical solution, the induction correction component includes a weight sensing chamber; a weight sensing chamber is provided inside the carrying platform, and a pressure sensing plate is provided in the weight sensing chamber. When a flat plate or culture dish is placed on the carrying platform and contacted by the clamping member, the weight is transferred to the pressure sensing plate. A lever is connected below the pressure sensing plate, and the fulcrum of the lever is located at a fixed position inside the carrying platform. The pressure sensing plate is connected to one end of the lever, and the other end is connected to an elastic adjustment connecting rod, which passes through the carrying platform and is connected to the elastic adjustment unit inside the buffer pad.

[0021] When the pressure sensing plate is subjected to pressure generated by plates or culture dishes of different weights, the lever rotates around the fulcrum, driving the elastic adjustment link to move, thereby changing the state of the elastic adjustment unit inside the buffer pad and adjusting the elastic modulus of the buffer pad.

[0022] As a further technical solution, the buffer pad includes an outer rubber layer and an elasticity adjustment unit, the elasticity adjustment unit is located inside the outer rubber layer, and the elasticity adjustment unit includes:

[0023] An adjusting spring, one end of which is fixed on the base layer and the other end is fixed on the movable base plate;

[0024] a base layer fixedly bonded to the inner side wall of the outer rubber layer away from the clamping member;

[0025] A movable base plate is movably arranged in the outer rubber layer and contacts the moving member with a wedge-shaped tail, and the bottom of the moving member is connected to the elastic adjustment connecting rod;

[0026] When the elastic adjustment link is moved by force, it drives the moving part to press the movable base plate upward to change the effective working length of each adjustment spring.

[0027] As a further technical solution, an auxiliary vibration component is also provided at the bottom of the supporting platform, and the auxiliary vibration component includes a plurality of electromagnetic vibrators, each of which is arranged between the outer sleeve and the inner telescopic rod; each of the electromagnetic vibrators is electrically connected to a push-type switch, and the push-type switch is arranged on one of the outer sleeves, and a fixed shaft is also provided on the outer sleeve, and a transmission lever is connected to the fixed shaft through a torsion spring, and a trigger protrusion is provided on the side of one of the racks, and one end of the transmission lever is located on the moving path of the trigger protrusion, and the other end is against the push-type switch.

[0028] A method for detecting Campylobacter jejuni, comprising the following steps:

[0029] Step 1: Preparation;

[0030] Place the plate or culture dish containing Campylobacter jejuni to be tested on the load platform of the detector, ensuring that the plate or culture dish is placed stably;

[0031] Step 2: Horizontal adjustment;

[0032] The level sensor on the load-bearing platform detects the load-bearing platform's horizontal state in real time and transmits the detection signal to the control unit. Based on the received signal, the control unit calculates the telescopic length of the support column that needs to be adjusted and sends a control instruction to the corresponding drive motor No. 1. Drive motor No. 1 drives the transmission screw to rotate, causing the inner telescopic rod to extend and retract within the outer sleeve, thereby adjusting the level state of the load-bearing platform. During the adjustment process, the level sensor continuously monitors and provides feedback until the load-bearing platform reaches a horizontal state.

[0033] Step 3: Image acquisition;

[0034] When the carrying platform is adjusted to a horizontal and stable state, the control unit sends an image acquisition signal to the detection camera; the detection camera takes a picture of the plate or culture dish and captures an image containing Campylobacter jejuni colonies;

[0035] Step 4: Colony identification and counting;

[0036] The collected images are transmitted to a computer system, and the pre-processed images are analyzed using image analysis software. By identifying the shape, color, and size characteristics of the colonies, Campylobacter jejuni colonies are accurately distinguished from other impurities or bacteria. The identified Campylobacter jejuni colonies are then counted and the relevant data is recorded.

[0037] Step 5: Output the results;

[0038] The colony count results, test time and sample information data are displayed on the display screen of the detector;

[0039] Step 6: Cleaning and maintenance;

[0040] After the test is completed, remove the plate or culture dish and clean and disinfect the carrying platform to ensure the accuracy of the next test.

[0041] Beneficial effects of the present invention:

[0042] (1) The automatic leveling mechanism monitors the horizontal state of the carrying platform in real time through the level sensor, and the control unit accurately controls the No. 1 drive motor based on the monitoring data to adjust the telescopic length of the automatic support column, ensuring that the carrying platform always remains horizontal, so that the image collected by the detection camera will not be distorted due to the tilt of the platform. For the detection of Campylobacter jejuni colonies, accurate images are the basis for the analysis software to identify and count, thereby greatly improving the accuracy of the test results. For example, in the detection of micro-colonies, a horizontal carrying platform can avoid the distortion of the colony morphology in the image and prevent the analysis software from misjudging.

[0043] (2) The inductive correction component can accurately convert the weight change of the plate or culture dish into a control signal for the elastic adjustment unit of the buffer pad through the pressure sensing plate, lever and elastic adjustment link in the weight sensing chamber; the adjustment spring in the elastic adjustment unit changes the effective working length through the movement of the movable base plate, thereby achieving precise adjustment of the elastic modulus of the buffer pad; for heavier samples, the elastic modulus is increased to provide sufficient support; for lighter samples, the elastic modulus is reduced to achieve gentle clamping, thereby improving the adaptability to samples of different weights and ensuring the stability of the sample during the detection process. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] The present invention will be further described below with reference to the accompanying drawings.

[0045] Figure 1 is a three-dimensional schematic diagram of the present invention;

[0046] Figure 2 for Figure 1 A three-dimensional diagram from another angle;

[0047] Figure 3 for Figure 1 Schematic diagram of the structure of the automatic support column;

[0048] Figure 4 It is a schematic diagram of the structure of the cushion and the induction correction component;

[0049] Figure 5 It is a structural diagram of the drive component;

[0050] Figure 6 This is a schematic diagram of the structure of the auxiliary vibration component of the present invention

[0051] Figure 7 A diagram showing the steps of the method of the present invention.

[0052] Description of the accompanying drawings: 1. Detection box; 2. Load-bearing platform; 3. Automatic level adjustment mechanism; 31. Automatic support column; 311. Outer sleeve; 312. Inner telescopic rod; 313. Drive motor No. 1; 314. Transmission screw; 32. Level sensor; 4. Automatic clamping assembly; 41. Limiting groove; 42. Limiting slider; 43. Clamping member; 44. Elastic member; 45. Drive assembly; 451. Gear; 452. Rack; 453. Drive motor No. 2; 46. Buffer pad; 461. Outer rubber layer; 462. Adjustment spring; 463. Base layer; 464. Movable base plate; 465. Moving part; 47. Induction correction assembly; 471. Pressure sensing plate; 472. Lever; 473. Elastic adjustment link; 5. Auxiliary vibration assembly; 51. Trigger protrusion; 52. Transmission lever; 53. Push-type switch. DETAILED DESCRIPTION

[0053] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts shall fall within the scope of protection of the present invention.

[0054] See also Figure 1-Figure 7 As shown, the present invention is a Campylobacter jejuni detector, comprising a detection box 1, a detection camera is provided on the top of the detection box 1, a carrying platform 2 for placing a flat plate or a culture dish is provided in the bottom area of ​​the detection box 1 opposite to the detection camera, and an automatic leveling mechanism 3 is provided below the carrying platform 2, which is used to keep the carrying platform 2 in a horizontal state at all times.

[0055] The automatic level adjustment mechanism 3 includes an automatic support column 31; at least three independently retractable automatic support columns 31 are connected to the bottom of the carrying platform 2, and the automatic support columns 31 are controlled by a control unit; a level sensor 32 is provided on the carrying platform 2, and the level sensor 32 is electrically connected to the control unit. The level sensor 32 detects the horizontal state of the carrying platform 2 in real time and transmits the signal to the control unit. The control unit controls the operation of the No. 1 drive motor 313 according to the received signal, and adjusts the retractable length of the automatic support column 31 so that the carrying platform 2 always remains in a horizontal state.

[0056] In the present invention, the control unit pre-stores standard data for determining horizontality. When the level sensor 32 detects that the level of the support platform 2 exceeds the standard data range, the control unit activates the corresponding automatic support column 31 for adjustment. The control unit is also connected to the image acquisition and analysis system of the detector. After the support platform 2 is adjusted to a stable horizontal state, it sends a signal to the image acquisition and analysis system that allows detection. The specific process is as follows:

[0057] S1: Ensure that the detection box 1, detection camera, carrying platform 2, automatic leveling mechanism 3 (including automatic support column 31, level sensor 32, control unit, first drive motor 313, etc.) and image acquisition and analysis system of the Campylobacter jejuni detector are installed and connected properly; the control unit has pre-stored leveling standard data, such as the maximum allowable tilt angles θ(X_max) and θ(Y_max);

[0058] The acquisition process is:

[0059] The level sensor 32 outputs the tilt data of the carrying platform 2 in the X-axis and Y-axis directions, which are recorded as ΔX and ΔY (unit: mm) respectively; the lengths of the carrying platform 2 in the X-axis and Y-axis directions are LX and LY (unit: mm) respectively;

[0060] The calculation formula for the inclination angle θ(X) in the X-axis direction (unit: radians) is: θ(X) = arctan(ΔX / LX);

[0061] The calculation formula for the inclination angle θ(Y) in the Y-axis direction (unit: radian) is: θ(Y) = arctan((ΔY / LY);

[0062] S2, the tester places the plate or culture dish containing Campylobacter jejuni on the carrying platform 2, at which time the carrying platform 2 may be in a non-horizontal state;

[0063] S3, the horizontal detection is started, and the horizontal sensor 32 on the carrying platform 2 starts working, collecting the tilt data ΔX and ΔY of the carrying platform 2 in the X-axis and Y-axis directions in real time; the horizontal sensor 32 transmits the collected data to the control unit in the form of electrical signals.

[0064] S4, tilt determination: after receiving the data from the level sensor 32, the control unit calculates the tilt angles θ(X) and θ(Y) of the carrying platform 2 in the X-axis and Y-axis directions according to the above-mentioned tilt angle calculation formula;

[0065] The control unit compares the calculated θ(X) and θ(Y) with pre-stored horizontal judgment standard data θ(X_max) and θ(Y_max);

[0066] If θ(X)>θ(X_max) or θ(Y)>θ(Y_max), it is determined that the carrying platform 2 needs to be horizontally adjusted;

[0067] S5, adjustment calculation and instruction sending. If the carrying platform 2 needs to be adjusted, the control unit calculates the height h(i) that each automatic support column 31 needs to be adjusted according to the support column adjustment height calculation formula; the specific acquisition method is:

[0068] The distance from a certain support column on the carrying platform 2 to the rotation center in the X-axis direction is d(X_i), and the distance in the Y-axis direction is d(Y_i), where i represents the i-th automatic support column 31;

[0069] The height h(X_i) of the i-th support column that needs to be adjusted in the X-axis direction is: h(X_i) = d(X_i) × θ(X);

[0070] The height h(Y_i) of the i-th support column that needs to be adjusted in the Y-axis direction is: h(Y_i) = d(Y_i) × θ(Y);

[0071] The total adjustment height h(i) of the i-th support column is: h(i)=√〔h(X_i) 2 +h(Y_i) 2 〕;

[0072] The control unit sends a control instruction to the corresponding first drive motor 313 according to the height that each support column needs to be adjusted. The control instruction includes the forward and reverse rotation direction of the motor and the rotation angle. The extension length of the support column is controlled by controlling the rotation angle.

[0073] S6, support column adjustment. After the automatic support column 31 receives the command from the control unit, the first drive motor 313 starts to work. The output shaft of the first drive motor 313 drives the transmission screw 314 to rotate. According to the forward and reverse rotation of the motor, the inner telescopic rod 312 extends or shortens in the outer sleeve 311, thereby adjusting the height of the support column.

[0074] Since the connection between the load-bearing platform 2 and the support column is provided with a universal ball joint, the load-bearing platform 2 can flexibly change its angle as the height of the support column is adjusted, gradually tending to a horizontal state;

[0075] S7, real-time monitoring and feedback. During the support column adjustment process, the level sensor 32 continuously monitors the tilt data of the support platform 2 in real time and continuously transmits the data to the control unit;

[0076] The control unit continuously adjusts the control instructions to the first drive motor 313 according to the newly received data to achieve precise horizontal adjustment;

[0077] S8, horizontal stability judgment and detection is started. When the control unit receives the data of the horizontal sensor 32 and calculates that θ(X)≤θ(X_max) or θ(Y)≤θ(Y_max), the control unit determines that the carrying platform 2 has been adjusted to a horizontal and stable state;

[0078] The control unit sends a signal that can be detected to the image acquisition and analysis system of the detector;

[0079] S9, detection execution, after the image acquisition and analysis system receives the signal, the detection camera on the top of the detection box 1 takes a picture of the plate or culture dish on the carrying platform 2;

[0080] The collected images are transmitted to the computer system and processed and analyzed by specialized software to achieve accurate identification and counting of Campylobacter jejuni colonies, completing the entire detection process.

[0081] The automatic telescopic column is an electric telescopic rod, which includes an outer sleeve 311, an inner telescopic rod 312, a No. 1 drive motor 313, and a transmission screw 314 arranged between the outer sleeve 311 and the inner telescopic rod 312. The output shaft of the No. 1 drive motor 313 is connected to the transmission screw 314, and the No. 1 drive motor 313 drives the transmission screw 314 to rotate through forward and reverse rotation to achieve the extension and retraction of the inner telescopic rod 312 in the outer sleeve 311; the connection between the bearing platform 2 and the support column is provided with a universal ball joint to adapt to the angle change of the bearing platform 2 during the adjustment process.

[0082] In the present invention, when the tester installs the carrying platform 2 with the Campylobacter jejuni plate or culture dish placed on it into the test box 1, the level sensor 32 starts to monitor the horizontal state of the carrying platform 2 in real time and continuously transmits the detection signal to the control unit;

[0083] The control unit analyzes the signal from the level sensor 32 and sends a control command to the first drive motor 313 once it determines that the carrying platform 2 is in a non-level state;

[0084] After receiving the command, the output shaft of the No. 1 drive motor 313 starts to rotate, driving the transmission screw 314 connected to the output shaft to rotate; since the transmission screw 314 and the inner telescopic rod 312 are threaded together, the rotation of the screw causes the inner telescopic rod 312 to perform telescopic movement in the outer sleeve 311.

[0085] Because the bottom of the carrying platform 2 is connected to at least three such independently retractable automatic support columns 31, through the telescopic combination of different support columns, the carrying platform 2 changes its angle with the assistance of the universal ball joint and gradually tends to a horizontal state; during the adjustment process, the horizontal sensor 32 continuously monitors and feeds back new data to the control unit, and the control unit adjusts the telescopic amount of each support column in real time according to these data until the carrying platform 2 reaches a horizontal state.

[0086] On the one hand, the automatic leveling mechanism 3 precisely adjusts the horizontal position of the support platform 2, ensuring that the images captured by the detection camera are not distorted by the perspective of the support platform 2 due to its tilt. This improves the accuracy of the analysis software in identifying and counting Campylobacter jejuni colonies, providing a strong guarantee for the reliability of the detection results. For example, when detecting small colonies, if the support platform 2 is tilted, the colony morphology may be distorted in the image, causing the analysis software to misjudge. A horizontal support platform 2 can avoid this situation.

[0087] On the other hand, the existence of this mechanism reduces the need for inspectors to manually adjust the level of the carrying platform 2, which not only saves time but also reduces the risk of contamination that may be introduced by manual operation. The automatic level adjustment process is precisely controlled by the control unit according to the sensor data, which is more efficient and accurate than manual adjustment. It not only improves the inspection efficiency, but also ensures the accuracy and stability of the inspection, while reducing labor costs and operational risks.

[0088] The automatic clamping assembly 4 includes:

[0089] A limiting groove 41 is provided on the carrying platform 2;

[0090] There are two limiting sliders 42, which are symmetrically distributed and slidably connected to the limiting groove 41;

[0091] The clamping member 43 is provided in a one-to-one correspondence with the limiting slider 42 and is provided in a concave structure;

[0092] The elastic member 44 is disposed between the limiting slider 42 and the side wall of the limiting groove 41 .

[0093] The automatic clamping assembly 4 further includes a driving assembly 45, which is disposed on the carrying platform 2 and includes:

[0094] Gear 451 is rotatably arranged at the middle position of the carrying platform 2;

[0095] Two racks 452 are provided, symmetrically distributed on both sides of the limiting slide and meshing with the gear 451, and each rack 452 is fixed with a corresponding limiting slider 42;

[0096] The second driving motor 453 is disposed on the carrying platform 2 and is in driving connection with the gear 451 .

[0097] In the present invention, the inspector places the plate or culture dish on the carrying platform 2, and the automatic clamping component 4 can be started at this time, and the second driving motor 453 starts to work, and its output shaft drives the gear 451 connected to it to rotate; because the two racks 452 are symmetrically distributed on both sides of the limiting slide and mesh with the gear 451, the rotation of the gear 451 will drive the two racks 452 to make linear motion in opposite directions; each rack 452 is fixed with a corresponding limiting slider 42, so the movement of the rack 452 drives the limiting slider 42 to slide in the limiting slot 41, The limiting slider 42 is connected to the concave-shaped clamping member 43. As the limiting slider 42 slides, the two clamping members 43 gradually approach the plate or culture dish. When the clamping member 43 contacts the plate or culture dish, continued movement will cause the elastic member 44 to be stretched. The elastic member 44 acts as a buffer to prevent excessive clamping force from damaging the sample, while ensuring that the plate or culture dish is stably clamped in the center of the supporting platform 2. During the clamping process, if the clamping position needs to be adjusted, it can be achieved by controlling the forward and reverse rotation of the second drive motor 453. The elastic member 44 is a tension spring.

[0098] On the one hand, the automatic clamping assembly 4 can quickly and accurately clamp the plate or culture dish to the center of the support platform 2, effectively avoiding the problem of missing colonies due to inaccurate placement, which may cause some colonies to be in the imaging blind spot. Through the transmission of the second drive motor 453, gear 451, and rack 452, the clamping member 43 is precisely moved, improving the comprehensiveness of the detection. For example, the assembly can accurately adjust the position of plates or culture dishes of different sizes to ensure that all colonies can be captured by the detection camera.

[0099] On the other hand, the setting of the elastic member 44 plays a good buffering role during the clamping process; it can not only ensure that the plate or culture dish is firmly clamped, but also prevent damage to the sample due to excessive clamping force, thereby protecting the integrity of the sample; moreover, the automatic clamping component 4 reduces the operation of the test personnel to repeatedly adjust the sample placement, saves a lot of time, and reduces the probability of sample contamination; it not only improves the detection efficiency, but also ensures the detection quality, while protecting the sample, thereby improving the practicality and reliability of the entire Campylobacter jejuni detector.

[0100] As a further technical solution, a buffer pad 46 is installed on the side of the clamping member 43 that contacts the plate or culture dish, and the elastic adjustment of the buffer pad 46 based on the weight sensing of the plate or culture dish is achieved through the induction correction component 47.

[0101] The induction correction assembly 47 includes a weight sensing chamber; a weight sensing chamber is provided inside the carrying platform 2, and a pressure sensing plate 471 is provided in the weight sensing chamber. When a plate or culture dish is placed on the carrying platform 2 and contacted by the clamping member 43, the weight is transferred to the pressure sensing plate 471. A lever 472 is connected below the pressure sensing plate 471. The fulcrum of the lever 472 is located at a fixed position inside the carrying platform 2. The pressure sensing plate 471 is connected to one end of the lever 472, and the other end is connected to an elastic adjustment link 473. The elastic adjustment link 473 passes through the carrying platform 2 and is connected to the elastic adjustment unit inside the buffer pad 46.

[0102] When the pressure sensing plate 471 is subjected to pressure generated by plates or culture dishes of different weights, the lever 472 rotates around the fulcrum, driving the elastic adjustment link 473 to move, thereby changing the state of the elastic adjustment unit inside the buffer pad 46, thereby adjusting the elastic modulus of the buffer pad 46.

[0103] The cushion 46 includes an outer rubber layer 461 and an elasticity adjustment unit. The elasticity adjustment unit is located inside the outer rubber layer 461 and includes:

[0104] An adjusting spring 462, one end of which is fixed to the base 463 and the other end of which is fixed to the movable base 464;

[0105] The base layer 463 is fixedly bonded to the inner wall of the outer rubber layer 461 away from the clamping member 43;

[0106] The movable base plate 464 is movably disposed in the outer rubber layer 461 and contacts the wedge-shaped moving member 465 at the tail end. The bottom of the moving member 465 is connected to the elastic adjustment link 473.

[0107] When the elastic adjustment link 473 is moved by force, it drives the moving member 465 to press the movable base plate 464 upward to change the effective working length of each adjustment spring 462 .

[0108] In the present invention, when the inspector places a plate or culture dish cultured with Campylobacter jejuni on the carrying platform 2, the weight of the plate or culture dish is transmitted to the pressure sensing plate 471 in the weight sensing chamber inside the carrying platform 2. The pressure sensing plate 471 initially extends out of the carrying platform 2. After sensing the pressure, the pressure sensing plate 471 is pressed down, causing the lever 472 connected to it to rotate around the fulcrum located at a fixed position inside the carrying platform 2; the rotation of the lever 472 drives the elastic adjustment link 473 connected to the other end to move, and the elastic adjustment link 473 passes through the carrying platform 2 and is connected to the bottom of the moving part 465 with a wedge-shaped tail in the elastic adjustment unit inside the buffer pad 46. The movement of the elastic adjustment link 473 drives the moving part 465 to move upward Since the movable member 465 conflicts with the movable base plate 464, and the movable base plate 464 is connected to the adjusting spring 462 fixed on the base 463, the movable member 465 presses the movable base plate 464 upward, and the movable base plate 464 slides or tilts along the axial direction of the adjusting spring 462. As the movable base plate 464 slides, the effective working length of the entire or lower middle adjusting spring 462 changes, thereby achieving the adjustment of the elastic modulus of the buffer pad 46. When the elastic adjusting link 473 pushes the movable base plate 464 to shorten the effective working length of the adjusting spring 462, the stiffness of the spring increases, and the overall elastic modulus of the buffer pad 46 increases; conversely, when the effective working length increases, the spring stiffness decreases, and the elastic modulus decreases;

[0109] On the one hand, the elastic modulus of the buffer pad 46 can be accurately adjusted according to the weight of the plate or culture dish; for heavier samples, the greater pressure causes the pressure sensing plate 471 to trigger the lever 472, driving the elastic adjustment link 473 to move more significantly, and the moving part 465 squeezes the movable base plate 464 more forcefully, greatly shortening the effective working length of the adjustment spring 462, increasing the elastic modulus of the buffer pad 46, and providing sufficient supporting force to prevent the sample from being damaged due to excessive deformation of the buffer pad 46; for lighter samples, the smaller pressure causes the effective working length of the adjustment spring 462 to change less, and the buffer pad 46 maintains a relatively soft elasticity, gently clamping the sample to avoid damage to the sample.

[0110] On the other hand, by utilizing simple mechanical linkage, the interaction between the lever 472, the elastic adjustment link 473, the moving part 465 and the movable base plate 464 realizes the elastic adjustment function. The entire structure is simple and reliable, which reduces the manufacturing and maintenance costs of the instrument. At the same time, the outer rubber layer 461 cooperates with the internal elastic adjustment unit. The outer rubber layer 461 provides basic buffering and protection, and the internal elastic adjustment unit dynamically adjusts the elasticity according to the sample weight. The two work together to meet the diverse clamping requirements of samples of different weights, and improve the stability and safety of the sample detection process through precise elastic adjustment, thereby ensuring the accuracy of the test results and optimizing the overall performance of the instrument, achieving multiple goals at one stroke.

[0111] An auxiliary vibration component 5 is also provided at the bottom of the supporting platform 2, and the auxiliary vibration component 5 includes a plurality of electromagnetic vibrators, each of which is arranged between the outer sleeve 311 and the inner telescopic rod 312; each of the electromagnetic vibrators is electrically connected to the push-type switch 53, and the push-type switch 53 is arranged on one of the outer sleeves 311, and the outer sleeve 311 is also provided with a fixed shaft, and a transmission lever 52 is rotatably connected to the fixed shaft through a torsion spring, and a trigger protrusion 51 is provided on the side of one of the racks 452, and one end of the transmission lever 52 is located on the moving path of the trigger protrusion 51, and the other end is against the push-type switch 53.

[0112] In the present invention, the electromagnetic vibrator is mainly integrated at the bottom of the transmission screw 314, and the vibration frequency is 20-50Hz. When the detection is completed and the clamping member 43 is released, the control unit drives the support column to quickly and slightly extend and retract (±0.1mm, 5 times), and the electromagnetic vibrator works synchronously to generate vertical micro-vibration; the vibration amplitude is monitored in real time by the horizontal sensor 32 to ensure that the horizontal state of the platform is not destroyed, that is, the vibration frequency is higher than the response frequency of the horizontal adjustment system, forming a high-frequency filter; on the one hand, the existing driving structure of the support column is used to achieve vibration without the need for an additional power source; on the other hand, the vibration parameters are dynamically decoupled from the stability of the platform to avoid the influence of traditional vibration devices on imaging accuracy; finally, after a long period of detection, the culture dish is prevented from adhering to the carrier platform 2 due to condensed water or adhesive, and is separated by slight vibration.

[0113] Specifically, when the second drive motor 453 is started, it drives the gear 451 to rotate, thereby causing the rack 452 to move in the limiting slide groove. As the rack 452 moves, the trigger protrusion 51 on the side of the rack 452 gradually approaches and pushes one end of the transmission lever 52. The transmission lever 52 rotates around the fixed axis, and its other end releases the push switch 53 on the electromagnetic vibrator, turning off the switch and stopping the electromagnetic vibrator. In this way, when the automatic clamping assembly 4 performs the clamping operation of the plate or culture dish, the auxiliary vibration assembly 5 remains stationary and does not interfere with the clamping process.

[0114] When the rack 452 returns to its initial position, the trigger protrusion 51 moves accordingly. At this time, since there is no push from the trigger protrusion 51, the transmission lever 52 returns to its initial position under the action of the torsion spring. The other end of the transmission lever 52 presses the push switch 53 on the electromagnetic vibrator again, closing the push switch 53. The electromagnetic vibrator starts working, generating micro-vibrations in the vertical direction, and assisting in separating the culture dish from the carrying platform 2.

[0115] A method for detecting Campylobacter jejuni, comprising the following steps:

[0116] Step 1: Preparation;

[0117] Place the plate or culture dish containing Campylobacter jejuni to be tested on the carrying platform 2 of the detector, ensuring that the plate or culture dish is placed stably;

[0118] Step 2: Horizontal adjustment;

[0119] The level sensor 32 on the carrying platform 2 detects the horizontal state of the carrying platform 2 in real time and transmits the detection signal to the control unit. Based on the received signal, the control unit calculates the telescopic length of the support column that needs to be adjusted and sends a control instruction to the corresponding drive motor 313. The drive motor 313 drives the transmission screw 314 to rotate, causing the inner telescopic rod 312 to extend and retract within the outer sleeve 311, thereby adjusting the horizontal state of the carrying platform 2. During the adjustment process, the level sensor 32 continuously monitors and provides feedback until the carrying platform 2 reaches a horizontal state.

[0120] Step 3: Image acquisition;

[0121] When the carrying platform 2 is adjusted to a horizontal and stable state, the control unit sends an image acquisition signal to the detection camera; the detection camera takes a picture of the plate or culture dish and captures an image containing Campylobacter jejuni colonies;

[0122] Step 4: Colony identification and counting;

[0123] The collected images are transmitted to a computer system, and the pre-processed images are analyzed using image analysis software. By identifying the shape, color, and size characteristics of the colonies, Campylobacter jejuni colonies are accurately distinguished from other impurities or bacteria. The identified Campylobacter jejuni colonies are then counted and the relevant data is recorded.

[0124] Step 5: Output the results;

[0125] The colony count results, test time and sample information data are displayed on the display screen of the detector;

[0126] Step 6: Cleaning and maintenance;

[0127] After the test is completed, the plate or culture dish is taken out and the carrying platform 2 is cleaned and disinfected to ensure the accuracy of the next test.

[0128] The above is a detailed description of an embodiment of the present invention. However, the content described is only a preferred embodiment of the present invention and should not be considered to limit the scope of the present invention. All equivalent changes and improvements made within the scope of the present invention should still fall within the scope of the patent coverage of the present invention.

Claims

1. A Campylobacter jejuni detector, comprising a detection box, a detection camera being provided on the top of the detection box, characterized in that: A carrying platform for placing a flat plate or culture dish is provided in the bottom area of ​​the detection box directly facing the detection camera, and an automatic leveling adjustment mechanism is provided under the carrying platform, which is used to keep the carrying platform in a horizontal state at all times; an automatic clamping component is also provided on the carrying platform, which is used to keep the flat plate or culture dish in the center position of the carrying platform.

2. The Campylobacter jejuni detector according to claim 1, characterized in that The automatic level adjustment mechanism includes an automatic support column; at least three independently retractable automatic support columns are connected to the bottom of the carrying platform, and the automatic support columns are controlled by a control unit; a level sensor is provided on the carrying platform, and the level sensor is electrically connected to the control unit. The level sensor detects the horizontal state of the carrying platform in real time and transmits the signal to the control unit. The control unit controls the operation of the No. 1 drive motor according to the received signal, and adjusts the retractable length of the automatic support column to keep the carrying platform always in a horizontal state.

3. The Campylobacter jejuni detector according to claim 2, characterized in that: The automatic telescopic column is an electric telescopic rod, which includes an outer sleeve, an inner telescopic rod, a No. 1 drive motor, and a transmission screw arranged between the outer sleeve and the inner telescopic rod. The output shaft of the No. 1 drive motor is connected to the transmission screw, and the No. 1 drive motor drives the transmission screw to rotate in the forward and reverse directions to achieve the extension and retraction of the inner telescopic rod in the outer sleeve; the connection between the load-bearing platform and the support column is provided with a universal ball joint to adapt to the angle change of the load-bearing platform during the adjustment process.

4. The Campylobacter jejuni detector according to claim 2, characterized in that: The automatic clamping assembly comprises: A limiting groove is provided on the carrying platform; There are two limit sliders, which are symmetrically distributed and slidably connected in the limit slot; The clamping member is provided in a one-to-one correspondence with the limit slider and is provided in a concave structure; The elastic member is arranged between the limiting slider and the side wall of the limiting groove.

5. The Campylobacter jejuni detector according to claim 4, characterized in that: The automatic clamping assembly further includes a driving assembly, which is arranged on the carrying platform and includes: A gear is rotatably arranged at the middle position of the bottom surface of the carrying platform; Two racks are provided, symmetrically distributed on both sides of the limiting slide and meshing with the gear, and each rack is fixed with a corresponding limiting slider; The second driving motor is arranged on the bearing platform and is connected to the gear transmission.

6. The Campylobacter jejuni detector according to claim 4 or 5, characterized in that: A buffer pad is installed on the side of the clamping member that contacts the flat plate or the culture dish, and the elastic adjustment of the buffer pad is achieved based on the weight sensing of the flat plate or the culture dish through the induction correction component.

7. The Campylobacter jejuni detector according to claim 6, characterized in that The induction correction assembly includes a weight sensing chamber; a weight sensing chamber is provided inside the carrying platform, and a pressure sensing plate is provided in the weight sensing chamber. When a plate or a culture dish is placed on the carrying platform and contacted by the clamping member, the weight is transferred to the pressure sensing plate. A lever is connected below the pressure sensing plate, and the fulcrum of the lever is located at a fixed position inside the carrying platform. The pressure sensing plate is connected to one end of the lever, and the other end is connected to an elastic adjustment connecting rod, which passes through the carrying platform and is connected to the elastic adjustment unit inside the buffer pad. When the pressure sensing plate is subjected to pressure generated by plates or culture dishes of different weights, the lever rotates around the fulcrum, driving the elastic adjustment link to move, thereby changing the state of the elastic adjustment unit inside the buffer pad and adjusting the elastic modulus of the buffer pad.

8. The Campylobacter jejuni detector according to claim 7, characterized in that: The buffer pad includes an outer rubber layer and an elasticity adjustment unit, the elasticity adjustment unit is located inside the outer rubber layer, and the elasticity adjustment unit includes: An adjusting spring, one end of which is fixed on the base layer and the other end is fixed on the movable base plate; a base layer fixedly bonded to the inner side wall of the outer rubber layer away from the clamping member; A movable base plate is movably arranged in the outer rubber layer and contacts the moving member with a wedge-shaped tail, and the bottom of the moving member is connected to the elastic adjustment connecting rod; When the elastic adjustment link is moved by force, it drives the moving part to press the movable base plate upward to change the effective working length of each adjustment spring.

9. The Campylobacter jejuni detector according to claim 3, characterized in that: An auxiliary vibration component is also provided at the bottom of the supporting platform, and the auxiliary vibration component includes a plurality of electromagnetic vibrators, each of which is provided between the outer sleeve and the inner telescopic rod; each of the electromagnetic vibrators is electrically connected to a push-type switch, and the push-type switch is provided on one of the outer sleeves, and a fixed shaft is also provided on the outer sleeve, and a transmission lever is connected to the fixed shaft through a torsion spring, and a trigger protrusion is provided on the side of one of the racks, and one end of the transmission lever is located on the moving path of the trigger protrusion, and the other end is against the push-type switch.

10. A method for detecting Campylobacter jejuni, characterized in that: The method is applicable to the Campylobacter jejuni detector according to any one of claims 1 to 8, and comprises the following steps: Step 1: Preparation; Place the plate or culture dish containing Campylobacter jejuni to be tested on the load platform of the detector, ensuring that the plate or culture dish is placed stably; Step 2: Horizontal adjustment; The level sensor on the load-bearing platform detects the level of the load-bearing platform in real time and transmits the detection signal to the control unit. Based on the received signal, the control unit calculates the telescopic length of the support column that needs to be adjusted and sends a control instruction to the corresponding drive motor No.

1. The drive motor No. 1 drives the transmission screw to rotate, causing the inner telescopic rod to extend and retract within the outer sleeve, thereby adjusting the level of the load-bearing platform. During the adjustment process, the level sensor continuously monitors and provides feedback until the carrying platform reaches a horizontal state; Step 3: Image acquisition; When the carrying platform is adjusted to a horizontal and stable state, the control unit sends an image acquisition signal to the detection camera; The detection camera takes a picture of the plate or culture dish and captures an image containing Campylobacter jejuni colonies; Step 4: Colony identification and counting; The collected images are transmitted to a computer system, and the pre-processed images are analyzed using image analysis software. By identifying the shape, color, and size characteristics of the colonies, Campylobacter jejuni colonies are accurately distinguished from other impurities or bacteria. The identified Campylobacter jejuni colonies are then counted and the relevant data is recorded. Step 5: Result output; The colony count results, test time and sample information data are displayed on the display screen of the detector; Step 6: Cleaning and maintenance; After the test is completed, remove the plate or culture dish and clean and disinfect the carrying platform to ensure the accuracy of the next test.

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