Application of composition containing probiotics in preparation of products for improving sleep

Abstract

The invention provides application of a composition containing probiotics in preparation of a product for improving sleep, and relates to the field of probiotics. The invention provides an application of a composition containing probiotics. The application is to prepare a product for improving sleep. The step of improving the sleep comprises the step of improving the sleep time; the composition comprises compound probiotic powder and lycium ruthenicum powder, the weight ratio of the compound probiotics to the lycium ruthenicum powder in the composition is (1: 6)-(1: 12). The composition can obviously prolong the sleep duration and can be used for preparing products for improving sleep.

Description

Technical Field

[0001] The present invention belongs to the field of probiotics, relates to a composition of probiotics and black wolfberry powder, and particularly relates to an application of the composition containing probiotics in the preparation of a product for improving sleep. Background Art

[0002] Probiotics can enhance immunity by influencing the body's specific and nonspecific immune responses. On the one hand, they can promote the proliferation and differentiation of lymphocytes (T cells) and lymphocytes (B cells) into plasma cells, producing cytokines such as IL-2 and IL-10, as well as antibodies such as IgA and IgG, participating in the regulation of the body's humoral and cellular immunity. On the other hand, they can also enhance the phagocytic activity of mononuclear phagocytes and macrophages, inducing the secretion of reactive oxygen species, lysosomal enzymes, and monokines, preventing the invasion of harmful substances such as pathogenic microorganisms and participating in nonspecific immune regulation. In addition to improving immunity, probiotics also have the potential to regulate and maintain the balance of intestinal flora, lower cholesterol, provide antioxidants, and reduce blood sugar.

[0003] Black wolfberry ( Lycium ruthenicum Murry (Lycium barbarum) is a perennial, deciduous, thorny shrub belonging to the genus Lycium barbarum in the Solanaceae family. It is rich in proanthocyanidins, anthocyanidins, Lycium barbarum polysaccharides, amino acids, B vitamins, and minerals. It boasts the highest anthocyanin content ever discovered in any plant, earning it the nickname "King of Anthocyanidins." Black wolfberry (Lycium barbarum) is a medicinal and edible plant endemic to western my country. Its representative nutrient, anthocyanidin, is a purple-red, water-soluble pigment extracted from the ripe fruit of the black wolfberry. It is a natural flavonoid polyphenol compound with numerous physiological benefits, including antioxidant activity, cardiovascular disease prevention, nonspecific immune enhancement, and memory improvement. Anthocyanidins in black wolfberry regulate the nervous system, alleviating nervous tension and anxiety, thereby improving sleep quality. Lycium barbarum polysaccharides in black wolfberry alleviate depression by reducing brain inflammation and reducing abnormal activity in the lateral habenula. Furthermore, anthocyanidins and Lycium barbarum polysaccharides can modulate levels of monoamine neurotransmitters (such as serotonin and dopamine), exerting antidepressant effects. Black wolfberry also boasts antioxidant, immune-boosting, vision-protecting, and anti-aging benefits. These combined health benefits provide additional support for improving sleep and combating depression. Therefore, it has broad application in the pharmaceutical, functional food, health food, and cosmetics industries.

[0004] Sleep is essential for proper brain function. Poor sleep quality can lead to a sallow complexion, slowed reaction times, and memory loss, not only impacting personal well-being and work but also contributing to chronic illnesses such as diabetes and cardiovascular and cerebrovascular diseases. Relying on sleeping pills and melatonin is like drinking poison to quench thirst, with significant side effects. They can also lead to dependence and directly inhibit the body's normal synthesis and secretion of melatonin, leading to sleep disorders. While many traditional Chinese medicine extracts have been discovered to improve sleep quality, due to various issues, including toxic side effects, there is currently no recognized dietary or medicinal supplement to improve sleep quality.

[0005] Prior art CN112970993A discloses a black wolfberry probiotic beverage and its preparation method. It comprises the following components by weight: 30-50% black wolfberry powder, 15-25% black wolfberry extract, 5-10% skim milk powder, 3-15% probiotic powder, and 10-30% excipients. The probiotic powder comprises the following components by weight: 1-3% Bifidobacterium lactis, 0.5-2% Bifidobacterium longum, 1-3% Bifidobacterium bifidum, 1-3% Lactobacillus plantarum, and 1-3% Lactobacillus rhamnosus. The excipients comprise the following components by weight: 1-2% polydextrose, 1-2% fructooligosaccharide, and 8-15% galacto-oligosaccharide. The probiotics used in this invention have excellent acid and bile resistance and intestinal colonization ability, ensuring their effective probiotic properties. The resulting probiotic solid beverage has no side effects, tastes good, and has the effect of enhancing immunity. The milk beverage provided by this technology has many ingredients, a complicated preparation process and high cost.

[0006] Prior art CN115299609A discloses a composition with sleep-improving properties, comprising the following components (1) and (2) in parts by weight: (1) 2-5 parts of black wolfberry extract and / or 2-5 parts of jujube seed extract and / or 2-5 parts of Chinese yam extract and / or 1-5 parts of rose petal extract; and (2) 0.05-0.5 parts of Bifidobacterium infantis dry powder. This technology has the disadvantage of having a poor sleep-improving effect. Summary of the Invention

[0007] The present invention addresses the problem of poor sleep improvement effect in the prior art and provides an application of a probiotic-containing composition in the preparation of a sleep-improving product. The present invention provides an application of a probiotic-containing composition, the application being for the preparation of a sleep-improving product; the sleep improvement includes improving sleep time; the composition includes composite probiotic powder and black wolfberry powder; the weight ratio of the composite probiotic to the black wolfberry powder in the composition is 1:6-1:12; the composite probiotic includes 2-5 types of probiotics, and the probiotics include at least lactobacilli and bifidobacteria; the preparation method of the black wolfberry powder includes: selecting dried black wolfberry fruit, soaking and beating, standing, removing floating matter, adding an antioxidant, adjusting the pH, mixing, filtering, freeze-drying, and crushing to obtain black wolfberry powder. The composition can significantly increase sleep duration and can be used to prepare a sleep-improving product.

[0008] To achieve the above object, the technical solution adopted by the present invention is as follows: In one aspect, the present invention provides an application of a composition containing probiotics, wherein the application is for preparing a product for improving sleep; the improving sleep includes improving sleep time; the composition includes composite probiotic powder and black wolfberry powder; The weight ratio of the composite probiotics to the black wolfberry powder in the composition is 1:6-1:12; The composite probiotics include 2-5 kinds of probiotics, and the probiotics include at least lactobacilli and bifidobacteria; The preparation method of the black wolfberry powder comprises the following steps: selecting dried black wolfberry fruits, soaking and beating the fruits, allowing the fruits to stand, removing floating matter, adding an antioxidant, adjusting the pH, mixing the fruits, filtering the fruits, freeze-drying the fruits, and crushing the fruits to obtain the black wolfberry powder.

[0009] Preferably, the improving sleep time comprises prolonging sleep duration and / or shortening sleep latency.

[0010] Preferably, the application also includes preparing products for improving metabolic imbalance, loss of appetite, excessive appetite, weight imbalance, endocrine disorders or emotional anxiety caused by sleep.

[0011] Preferably, the lactobacillus includes Lactobacillus plantarum and Lactobacillus rhamnosus.

[0012] Preferably, the content of live bacteria in the composite probiotic powder is ≥1.0×10 11 CFU / g, preferably 1.6×10 11 CFU / g.

[0013] Preferably, the preparation method of the composite probiotics comprises: culturing each bacterium individually or in a mixed culture, collecting the bacterial bodies, mixing them with dextrin, and drying them.

[0014] Preferably, the bifidobacterium in the composite probiotics is Bifidobacterium animalis lactis subspecies, and the lactobacilli in the composite probiotics are composed of Lactobacillus plantarum and Lactobacillus rhamnosus; the mass ratio of the bacterial powders of Lactobacillus plantarum, Lactobacillus rhamnosus and Bifidobacterium animalis lactis subspecies in the bacterial bodies collected during the preparation process of the composite probiotics is 5-10:2-4:1-3; preferably 8:3:2.

[0015] Preferably, the dextrin includes any one or more of maltodextrin, cyclodextrin or corn dextrin.

[0016] Preferably, the composition further comprises oligofructose; the oligofructose accounts for 20%-40% of the total mass of the composition, preferably 30%.

[0017] Preferably, the product further comprises excipients, and the excipients are selected from any one of food excipients, health care product excipients or pharmaceutical excipients.

[0018] Compared with the prior art, the present invention has the following beneficial effects: The probiotic-containing composition provided by the present invention has a clean formula, a simple and reasonable preparation process, strong operability and applicability, can significantly prolong sleep time, improve sleep quality, and can be used to prepare products for improving sleep quality. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 The changes in sleep duration before and after modeling in mice, * indicates significant differences (P<0.05).

[0020] Figure 2 The body weight changes of mice in different groups.

[0021] Figure 3 Statistical graph of sleep duration of mice in different groups.

[0022] Figure 4 Statistical chart of sleep latency of mice in different groups. DETAILED DESCRIPTION

[0023] Unless otherwise specified, the raw materials used in the present invention are all common commercially available products, and their sources are not specifically limited.

[0024] The sources of the probiotics used in the present invention are shown in Table 1.

[0025] Table 1 Description of probiotic sources

[0026] Example 1: Preparation of a probiotic composition (1) Preparation of black wolfberry powder: First, remove the bad fruits and impurities, select dry wolfberries with bright color and no physical damage, add 15 times the weight of deionized water, soak for 45 minutes and beat for pulping. After the beating is completed, let it stand for 3 hours, remove the floating matter on the liquid surface and add 0.06% ascorbic acid. Use 1 mol / L citric acid solution to adjust the pH of the black wolfberry juice to 4.0, stir evenly and filter, vacuum freeze-dry at -40℃, crush and pass through a 50-mesh sieve to obtain black wolfberry powder.

[0027] (2) Preparation of compound probiotic powder: Lactobacillus plantarum powder, Lactobacillus rhamnosus powder, Bifidobacterium animalis subsp. lactis powder and maltodextrin were mixed in a mass ratio of 8:3:2:1, and the total viable bacterial count in the composite powder was ≥1.6×10 11 CFU / g.

[0028] (3) Preparation of probiotic composition Fully mix 45 parts of black wolfberry powder and 5 parts of compound probiotic powder according to mass proportions, and obtain a probiotic composition after passing the metal detection test.

[0029] Example 2: Preparation of a probiotic composition Black wolfberry powder and composite probiotic powder were prepared according to the method of Example 1. 45 parts of black wolfberry powder, 15 parts of oligofructose and 5 parts of composite probiotic powder were fully mixed according to the weight ratio. After the metal detection passed, the probiotic composition was obtained.

[0030] Effect Example 1: Experiment on improving sleep of mice with a probiotic composition S1. Animal grouping For this experiment, 90 healthy, 6-week-old male KM mice (weighing 32.0 g to 45.0 g) were purchased from Hangzhou Qizhen Laboratory Animal Technology Co., Ltd. Mice were housed in the animal room of the Experimental Animal Center of East China University of Science and Technology at an ambient temperature of 25 ± 2°C, a humidity of 50 ± 5%, with a 12-hour light-dark cycle, and free access to food and water. After one week of adaptive feeding, the mice were randomly divided according to body weight into a normal group, a model group, a diazepam group (positive drug control), a low-dose black wolfberry group (black wolfberry powder prepared in Example 1), a high-dose black wolfberry group (black wolfberry powder prepared in Example 1), a low-dose probiotic group (composite probiotic powder prepared in Example 1), a high-dose probiotic group (composite probiotic powder prepared in Example 1), a low-dose composition group (probiotic composition prepared in Example 1), and a high-dose composition group (probiotic composition prepared in Example 1), with 10 mice per group.

[0031] S2. Insomnia mouse model establishment and drug treatment Three days before the formal experiment, a sodium pentobarbital suprathreshold dose reversal test was performed. Except for the normal group, mice in all groups received intraperitoneal injections of 300 mg / kg parachlorophenylalanine (PCPA) starting on day 1 for two consecutive days. The sodium pentobarbital suprathreshold dose reversal test was repeated on day 3. The sleep duration of the same mice was compared with that before model establishment to assess model efficacy. On day 10, PCPA was administered again intraperitoneally at a concentration of 200 mg / kg to consolidate the model. The drug-treated groups were gavaged once daily from day 1 to day 14. Ten minutes after gavage on day 14, a sodium pentobarbital reversal test was performed. The treatment methods for each group are shown in Table 2. For groups 3–9, the corresponding mass of solid powder was weighed and dissolved in 0.2 mL of sterile deionized water before gavage.

[0032] Table 2 Mouse grouping and treatment methods

[0033] S3. Data Analysis All numerical values ​​in the experiment are expressed as (mean ± standard deviation). The results were analyzed using the statistical software GraphPadPrism 10. One-way analysis of variance (ANOVA) was used to compare the differences between different groups, and the significance level was set at p <0.05.

[0034] S4. Experimental Results (1) Determination of the threshold dose of pentobarbital sodium Three days prior to the official dosing, a study was conducted on the suprathreshold and subthreshold doses of pentobarbital sodium in mice for the sleep-onset test. Ten mice were intraperitoneally injected with varying concentrations of pentobarbital sodium. Loss of the righting reflex within 30 seconds was used as the criterion for sleep onset. The suprathreshold and subthreshold doses of pentobarbital sodium were determined. As shown in Table 3, the final suprathreshold dose was 52 mg / kg, and the subthreshold dose was 37 mg / kg.

[0035] Table 3 Threshold dose of pentobarbital sodium

[0036] (2) The mouse insomnia model was established.

[0037] The mice were intraperitoneally injected with 300 mg / kg PCPA starting from the first day for two consecutive days, and a sodium pentobarbital suprathreshold dose-induced reversal experiment was performed on the third day. The sleep duration of the same mice was compared with that before modeling. Figure 1 It can be seen that the sleep duration of mice after modeling was significantly different from that of mice before modeling ( p <0.05), thus confirming that the insomnia mouse model was successfully established.

[0038] (3) Effect of the composition on body weight changes in mice The experimental results are as follows Figure 2 As shown, over the 14-day experimental period, weight changes in all groups of mice were generally similar. However, mice in the model and diazepam groups experienced significant weight loss at the beginning of the experiment. In contrast, mice in the low- and high-dose groups receiving the composition experienced less weight loss, which gradually recovered to near-normal levels in the later stages of the experiment. To validate the effectiveness of the insomnia model, mice were intraperitoneally injected with 200 mg / kg PCPA on day 10. Results showed that mice in the model group experienced significant weight loss on day 11, likely due to the negative effects of PCPA on metabolism and appetite, while weight changes in the other groups were not significant, suggesting that the composition may help maintain stable weight in mice and mitigate weight loss caused by insomnia. The results demonstrate that the composition has potential to improve weight stability in mice, particularly in the composition-treated groups, where weight loss in the model group was significantly less pronounced. This suggests that the black wolfberry probiotic composition has potential positive effects on metabolism and appetite, providing support for its potential health benefits in improving sleep and combating depression.

[0039] (4) Effect of the composition on sleep quality of mice Figure 3 Table 4 and Table 5 show the sleep duration of mice in different groups. Compared with the normal group, the model group showed significantly reduced sleep duration, indicating insomnia symptoms. The diazepam group showed significantly higher sleep duration than the model group, demonstrating its sleep-improving effect. The high-dose black wolfberry probiotic composition group also showed significantly higher sleep duration than the model group. Furthermore, the high-dose black wolfberry and probiotic composition groups also showed significantly higher sleep duration, indicating a synergistic effect of the composition. Figure 4 Table 4 shows the sleep latency of mice in different groups. The sleep latency of mice in the model group was the longest, while that in the diazepam group was the shortest. The high-dose and low-dose black wolfberry probiotic composition groups were between the two groups, but the difference was not statistically significant ( p >0.05). The results showed that the black wolfberry probiotic composition had significant potential in improving the sleep quality of mice and could prolong sleep duration, but had no significant effect on shortening sleep latency.

[0040] Table 4 Effects of different treatments on sleep duration and sleep latency in mice

[0041] Note: Data in the same column marked with the same letter indicate that there is no significant difference between the data ( P >0.05, different letters indicate significant differences between the data ( P <0.05).

[0042] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions of the technical solution of the present invention by ordinary technicians in this field do not deviate from the essence and scope of the technical solution of the present invention.

Claims

1. An application of a composition containing probiotics, characterized in that, The application is to prepare a product for improving sleep; the improvement of sleep includes improving sleep time; the composition includes compound probiotic powder and black wolfberry powder; The weight ratio of the composite probiotics to the black wolfberry powder in the composition is 1:6-1:12; The composite probiotics include 2-5 kinds of probiotics, and the probiotics include at least lactobacilli and bifidobacteria; The preparation method of the black wolfberry powder comprises the following steps: selecting dried black wolfberry fruits, soaking and beating the fruits, allowing the fruits to stand, removing floating matter, adding an antioxidant, adjusting the pH, mixing the fruits, filtering the fruits, freeze-drying the fruits, and crushing the fruits to obtain the black wolfberry powder.

2. The use according to claim 1, characterized in that The improving of sleep time includes prolonging sleep duration and / or shortening sleep latency.

3. The use according to claim 1, characterized in that The application also includes preparing products for improving metabolic imbalance, loss of appetite, excessive appetite, weight imbalance, endocrine disorder or emotional anxiety caused by sleep.

4. The use according to claim 1, characterized in that The lactobacillus includes Lactobacillus plantarum and Lactobacillus rhamnosus.

5. The use according to claim 4, characterized in that The content of live bacteria in the composite probiotic powder is ≥1.0×10 11 CFU / g, preferably 1.6×10 11 CFU / g.

6. The use according to claim 5, characterized in that The preparation method of the composite probiotics comprises: culturing each bacterium individually or in a mixed culture, collecting the bacterial bodies, mixing them with dextrin, and drying them.

7. The use according to claim 6, characterized in that The bifidobacterium in the composite probiotics is Bifidobacterium animalis lactis subspecies, and the lactobacilli in the composite probiotics are composed of Lactobacillus plantarum and Lactobacillus rhamnosus. The mass ratio of bacterial powder of Lactobacillus plantarum, Lactobacillus rhamnosus and Bifidobacterium animalis lactis subspecies in the bacterial bodies collected during the preparation process of the composite probiotics is 5-10:2-4:1-3, preferably 8:3:

2.

8. The use according to claim 7, characterized in that The dextrin includes any one or more of maltodextrin, cyclodextrin or corn dextrin.

9. The use according to claim 1, characterized in that The composition further comprises oligofructose; the oligofructose accounts for 20%-40% of the total mass of the composition, preferably 30%.

10. The use according to claim 1, characterized in that The product also includes auxiliary materials, which are selected from any one of food auxiliary materials, health care product auxiliary materials or pharmaceutical auxiliary materials.

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