Nerve-stabilizing composition, preparation method, preparation and quality control and content determination method thereof

Abstract

The invention belongs to the field of traditional Chinese medicine, and provides a mind-stabilizing composition, a preparation method, a preparation and a quality control and content determination method thereof, the mind-stabilizing composition is prepared from the following raw materials in parts by weight: 5-10 parts of gastrodia elata, 5-10 parts of stiff silkworm, 3-6 parts of uncaria, 2-4 parts of ephedra, 5-10 parts of fried spina date seed, 2-4 parts of tabasheer, 10-20 parts of poria cocos and 3-6 parts of rhizoma acori graminei. The tranquilizing composition is prepared from eight traditional Chinese medicines, namely gastrodia elata, stiff silkworm, uncaria, ephedra, fried spina date seed, tabasheer, poria cocos and rhizoma acori graminei, and has the effects of calming liver wind, tonifying spleen, clearing heat, reducing phlegm, soothing nerves and stopping movement. The traditional Chinese medicine is used for treating infantile touretic disorder (touretic disorder), liver-wind internal movement of hyperactivity and phlegm-heat disturbing spirit.

Description

Technical Field

[0001] The present invention belongs to the field of traditional Chinese medicine and relates to a stabilizing composition, a preparation method, a preparation and a quality control and content determination method thereof. Background Art

[0002] The information disclosed in this background technology section is only intended to enhance understanding of the overall background of the invention and should not necessarily be regarded as an admission or any form of suggestion that the information constitutes the prior art already known to those skilled in the art.

[0003] Pediatric tic disorder is a neurodevelopmental disorder characterized by involuntary, repetitive movements or vocalizations, commonly occurring in children aged 5 to 12 years. Its core manifestations are motor tics (such as blinking and shrugging) and vocal tics (such as throat clearing and screaming). Symptoms can be transient or persistent and are often ameliorated through comprehensive interventions.

[0004] ADHD, the full name of which is Attention Deficit Hyperactivity Disorder (ADHD), is a common neurodevelopmental disorder characterized by inattention, hyperactivity, impulsive behavior, etc. It is common in children and may persist into adulthood, affecting learning, social interaction and daily life.

[0005] Currently, Chinese medicine often uses Liuwei Dihuang Pills, Zuogui Pills, Anshenbunao Liquid or Suanzaoren Decoction for conditioning. These medicines can calm the mind, harmonize qi and blood, and help improve tic disorders and hyperactivity symptoms in children.

[0006] However, for children's tic disorders (tics), ADHD caused by internal liver wind, phlegm-heat disturbing the mind and other symptoms, more targeted drugs still need to be developed. Summary of the Invention

[0007] To address the above-mentioned issues, the present invention provides a tranquilizing composition, preparation method, formulation, and quality control and content determination methods. This tranquilizing composition comprises eight Chinese herbs: Gastrodia elata, Bombyx batryticatus, Uncaria rhynchophylla, Ephedra sinica, stir-fried Ziziphus jujuba seeds, Radix dahliae, Poria cocos, and Acorus calamus. It has the effects of calming the liver and extinguishing wind, strengthening the spleen, clearing heat and resolving phlegm, and calming the mind. It is used to treat tic disorders (tics) and ADHD in children characterized by internalized liver wind and disturbed mind due to phlegm-heat.

[0008] In order to achieve the above object, the present invention adopts the following technical solutions: The first aspect of the present invention provides a stabilizing composition, which is composed of the following raw materials in parts by weight: 5-10 parts of Gastrodia elata, 5-10 parts of Bombyx batryticatus, 3-6 parts of Uncaria rhynchophylla, 2-4 parts of Ephedra, 5-10 parts of stir-fried Ziziphus jujuba seeds, 2-4 parts of Radix dahliae, 10-20 parts of Poria cocos, and 3-6 parts of Acorus calamus.

[0009] The second aspect of the present invention provides a method for preparing a stabilizing composition, comprising: Mix Gastrodia elata, Bombyx batryticatus, Uncaria rhynchophylla, Ephedra sinica, stir-fried Ziziphus jujuba seeds, Rhizoma jasminoides, Poria cocos and Acorus calamus evenly, add water and boil to obtain the medicine.

[0010] The third aspect of the present invention provides a traditional Chinese medicine preparation for stabilizing the mind, comprising: the above-mentioned stabilizing composition.

[0011] A fourth aspect of the present invention provides a method for controlling the quality of a stabilizing composition, comprising: Uncaria rhynchophylla, Bombyx batryticatus, Semen ziziphi spinulosae, Ephedra sinica and Acorus tatarinowii were used as control herbs respectively, and corresponding test sample solutions were prepared at the same time, and thin layer chromatography was used for identification to obtain the product.

[0012] A fifth aspect of the present invention provides a method for determining the content of a Wenshen composition, using octadecylsilane bonded silica gel as a filler, acetonitrile as mobile phase A, and 0.05% phosphoric acid solution as mobile phase B, for gradient elution; The conditions for gradient elution are: 0-15 min, mobile phase A 2%; 15-17 min, mobile phase A 2%-15%; 17-25 min, mobile phase A 15%-70%; 25 min-25.01 min, mobile phase A 70%-2%; 25.01-35 min, mobile phase A 2%.

[0013] Beneficial effects of the present invention (1) The present invention has the effects of calming the liver and extinguishing wind, strengthening the spleen, clearing heat and resolving phlegm, and calming the mind and stopping movement. It is used to treat tic disorders (tics) and ADHD caused by internal liver wind and phlegm-heat disturbing the mind. Among them, Gastrodia elata is sweet in taste, neutral in nature, and enters the liver meridian. It has the effects of calming the liver and extinguishing wind, massaging the liver to stop spasms, suppressing liver yang, and dispelling wind and unblocking the meridians. Uncaria rhynchophylla is sweet in taste, neutral in nature and slightly cool in nature, and enters the liver and heart meridians. It has the effects of clearing heat and calming the liver, extinguishing wind and calming convulsions, and can treat epilepsy and spasms in children, and dizziness and vertigo in adults. Bombyx batryticatus is pungent and salty in taste, neutral in nature, and enters the liver, lung, and stomach meridians. It has the effects of dispelling wind and relieving spasms, resolving phlegm and dispersing stagnation, and can be used to treat epilepsy and palpitations in children. The three medicines are combined into one, which has the effects of clearing heat and calming the liver and extinguishing wind, massaging the liver to stop movement. All three are monarch drugs. Bombyx batryticatus, a popular animal medicine for calming wind and relieving spasms, is primarily composed of proteins. It also contains peptides, amino acids, polysaccharides, and trace elements. Uncaria rhynchophylla's main chemical components include alkaloids, triterpenes, saponins, and flavonoids. Modern pharmacological experiments have shown that the chemical components of Uncaria rhynchophylla plants possess a variety of effects, including antihypertensive, analgesic, anti-inflammatory, anti-tumor, sedative, and antioxidant properties. Gastrodia elata's main active ingredient is gastrodin, which is present in high concentrations. p-Hydroxybenzyl alcohol is a gastrodin aglycone. Gastrodin and its aglycone (p-Hydroxybenzyl alcohol) are both quality-controlled components of Gastrodia elata in the Chinese Pharmacopoeia and form the basis for its efficacy. They exhibit significant physiological activity in treating conditions such as hypnosis and sedation, anticonvulsant effects, anti-vertigo effects, analgesia, depression, anxiety, cognitive impairment, myocardial cell protection, blood pressure reduction, and antithrombotic and antiplatelet effects.

[0014] (2) The preparation method of the present invention is simple, practical, and easy to promote. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] The accompanying drawings, which constitute a part of the present invention, are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their description are used to explain the present invention and do not constitute improper limitations on the present invention.

[0016] Figure 1 Gastrodin-specific profile; Figure 2 Uncaria rhynchophylla-specific thin-layer chromatography; 1. Wenshen Granule 01 of Example 1, 20 μl; 2. Wenshen Granule 02 of Example 1, 20 μl; 3. Wenshen Granule 03 of Example 1, 20 μl; 4. Uncaria rhynchophylla control medicinal material, 10 μl; 5. Negative sample, 20 μl; 6. Blank solvent, 20 μl; Note: 01, 02, and 03 represent different batches of products; Figure 3 Bombyx batryticatus-specific thin-layer chromatography; 1. Wenshen Granule 01 of Example 1 5 μl; 2. Wenshen Granule 02 of Example 1 5 μl; 3. Wenshen Granule 03 of Example 1 5 μl; 4. Bombyx batryticatus control medicinal material 15 μl; 5. Negative sample 5 μl; 6. Blank solvent 5 μl; Figure 4Specific thin-layer chromatography of stir-fried Chinese jujube seeds; 1. Wenshen Granule 01 of Example 1, 5 μl; 2. Wenshen Granule 02 of Example 1, 5 μl; 3. Wenshen Granule 03 of Example 1, 5 μl; 4. Chinese jujube seed control medicinal material, 10 μl; 5. Spinosin reference substance, 10 μl; 6. Negative sample, 5 μl; Figure 5 Ephedra-specific thin-layer chromatography; 1. Wenshen Granule 01 of Example 1, 20 μl; 2. Wenshen Granule 02 of Example 1, 20 μl; 3. Wenshen Granule 03 of Example 1, 20 μl; 4. Ephedra control medicinal material, 5 μl; 5. Ephedrine hydrochloride reference substance, 5 μl; 6. Negative sample, 25 μl; Figure 6 Specific thin-layer chromatography of Acorus tatarinowii; 1. Wenshen Granule 01 of Example 1 30 μl; 2. Wenshen Granule 02 of Example 1 30 μl; 3. Wenshen Granule 03 of Example 1 30 μl; 4. Acorus tatarinowii control medicinal material 5 μl; 5. β-asarone reference substance 5 μl; 6. Negative sample 30 μl. DETAILED DESCRIPTION

[0017] It should be noted that the following detailed descriptions are exemplary and intended to provide further explanation of the present invention. Unless otherwise specified, all technical and scientific terms used in the present invention have the same meanings as those commonly understood by those skilled in the art to which the present invention belongs.

[0018] Unless otherwise defined, all professional and scientific terms used herein have the same meaning as those familiar to those skilled in the art. The reagents or raw materials used in the present invention can be purchased through conventional channels. Unless otherwise specified, the reagents or raw materials used in the present invention are used in accordance with conventional methods in the art or according to product specifications. Similarly, unless otherwise specified, the test methods of the present invention are also tested in accordance with conventional methods in the art or the common methods or standards in the industry. In addition, any methods and materials similar to or equivalent to the described contents can be applied to the inventive method. The preferred embodiments and materials described herein are for demonstration purposes only.

[0019] The invention provides a stabilizing composition, which is composed of the following raw materials in parts by weight: 5-10 parts of Gastrodia elata, 5-10 parts of Bombyx batryticatus, 3-6 parts of Uncaria rhynchophylla, 2-4 parts of Ephedra, 5-10 parts of stir-fried Ziziphus jujuba seeds, 2-4 parts of Radix dahliae, 10-20 parts of Poria cocos, and 3-6 parts of Acorus calamus.

[0020] The dosage of traditional Chinese medicine directly affects its efficacy and may even change its medicinal properties. Therefore, the present invention studies the dosage of each component. In some embodiments, the present invention is composed of the following raw materials in parts by weight: 5-7.5 parts of Gastrodia elata, 5-8 parts of Bombyx batryticatus, 3-4.5 parts of Uncaria rhynchophylla, 2-3 parts of Ephedra, 5-7.5 parts of stir-fried Ziziphus jujuba seeds, 2-3 parts of Radix dahliae, 10-15 parts of Poria cocos, and 3-4.5 parts of Acorus calamus to obtain better therapeutic effects.

[0021] The dosage of Chinese medicine needs to be adjusted comprehensively according to the constitution, condition, compatibility and drug properties. Therefore, in some embodiments, it is composed of the following raw materials in parts by weight: 7.5-10 parts of Gastrodia elata, 7.5-10 parts of Bombyx batryticatus, 4.5-6 parts of Uncaria rhynchophylla, 3-4 parts of Ephedra, 7.5-10 parts of stir-fried Ziziphus jujuba seeds, 3-4 parts of Radix dahliae, 15-20 parts of Poria cocos, and 4.5-6 parts of Acorus calamus, so as to achieve better therapeutic effects for different conditions.

[0022] The present invention provides a traditional Chinese medicine preparation for stabilizing the mind, comprising: the above-mentioned stabilizing the mind composition.

[0023] The present application does not impose any particular limitation on the dosage form of the Chinese medicine preparation. In some embodiments, the dosage form is selected from at least one of decoctions, pills, powders, ointments, lotions, pills, wines, and tablets.

[0024] Quality control affects the final effect and safety of Chinese medicinal materials. To this end, the present invention provides a quality control method for a Shen-sense composition, comprising: The corresponding test sample solutions were prepared with Uncaria rhynchophylla, Bombyx batryticatus, Semen Ziziphi spinulosae, Ephedra sinica and Acorus tatarinowii as control medicinal materials respectively, and the products were identified by thin layer chromatography.

[0025] In some embodiments, when using Suanzaoren semen as a reference medicinal material, spinosin is used to prepare a reference solution; spinosin (molecular formula C 28 H 32 O 15 ) is a national pharmaceutical standard substance certified by the China Food and Drug Inspection Institute. As a chemical reference substance in the 2020 edition of the "Chinese Pharmacopoeia", it is mainly used for the identification and content determination of medicinal materials such as Chinese jujube seeds.

[0026] In some embodiments, when ephedra is used as a reference medicinal material, ephedrine hydrochloride is used to prepare a reference solution; ephedrine hydrochloride is an alkaloid with a chemical formula of C 10 H 15 NO·HCl can be used to identify ephedra.

[0027] In some embodiments, when Acorus calamus is used as a reference medicinal material, β-asarone is used to prepare a reference solution. Acorus calamus rhizomes and leaves contain volatile oils, the main components of which are β-asarone and asarone. Therefore, β-asarone is used to prepare a reference solution.

[0028] The present invention provides a method for determining the content of a Wenshen composition, using octadecylsilane bonded silica gel as a filler, acetonitrile as mobile phase A, and 0.05% phosphoric acid solution as mobile phase B, for gradient elution; The conditions for gradient elution are: 0-15 min, mobile phase A 2%; 15-17 min, mobile phase A 2%-15%; 17-25 min, mobile phase A 15%-70%; 25 min-25.01 min, mobile phase A 70%-2%; 25.01-35 min, mobile phase A 2%.

[0029] The flow rate affects the separation degree, peak shape, analysis time and column efficiency of chromatographic peak separation. Therefore, the present invention studies the flow rate. In some embodiments, the flow rate is 1.0 ml per minute to obtain better detection effect.

[0030] Column temperature mainly affects the separation efficiency, retention time and peak shape of chromatographic peak separation. Therefore, the present invention studies the column temperature. In some embodiments, the column temperature is 30° C. to improve the separation efficiency.

[0031] The detection wavelength directly affects the signal response intensity and separation. Therefore, the present invention studies the detection wavelength. In some embodiments, the detection wavelength is 220 nm to obtain better signal response intensity and separation.

[0032] The injection volume directly affects the accuracy of the analysis results. Therefore, the present invention studies the injection volume. In some embodiments, the injection volume is 10 μl to obtain better accuracy.

[0033] The present invention will be further described in detail below with reference to specific embodiments. It should be noted that the specific embodiments are intended to explain the present invention rather than to limit it.

[0034] Example 1 Prescription: Gastrodia elata 5g, Bombyx batryticatus 5g, Uncaria rhynchophylla 3g, Ephedra sinica 2g, stir-fried Ziziphus jujuba seeds 5g, Radix jasminoides 2g, Poria cocos 10g, Acorus gramineus 3g.

[0035] Preparation: Decoction the above eight herbs twice. First, add 6 times the amount of water, soak for 30 minutes, and decoct for 30 minutes. Second, add 6 times the amount of water and decoct for 20 minutes. Combine the two filtrates, filter, and allow to stand. Concentrate the supernatant to a clear paste with a relative density of 1.10-1.15 (at 50°C). Add sucrose powder and dextrin to the paste, granulate, dry, and make 1500g of granules. Mix thoroughly and package to obtain Wenshen Granules.

[0036] Example 2 Prescription: Gastrodia elata 10g, Bombyx batryticatus 10g, Uncaria rhynchophylla 6g, Ephedra sinica 4g, stir-fried Ziziphus jujuba seeds 10g, Radix jasminoides 4g, Poria cocos 20g, Acorus gramineus 6g.

[0037] Preparation: Decoction the above eight herbs twice. First, add 6 times the amount of water, soak for 30 minutes, and decoct for 30 minutes. Second, add 6 times the amount of water and decoct for 20 minutes. Combine the two filtrates, filter, and allow to stand. Concentrate the supernatant to a clear paste with a relative density of 1.10-1.15 (at 50°C). Add sucrose powder and dextrin to the paste, granulate, dry, and make 1500g of granules. Mix thoroughly and package to obtain Wenshen Granules.

[0038] Example 3 Prescription: Gastrodia elata 7.5g, Bombyx batryticatus 7.5g, Uncaria rhynchophylla 4.5g, Ephedra sinica 3g, stir-fried Ziziphus jujuba seeds 7.5g, Radix jasminoides 3g, Poria cocos 15g, Acorus gramineus 4.5g.

[0039] Preparation: Decoction the above eight herbs twice. First, add 6 times the amount of water, soak for 30 minutes, and decoct for 30 minutes. Second, add 6 times the amount of water and decoct for 20 minutes. Combine the two filtrates, filter, and allow to stand. Concentrate the supernatant to a clear paste with a relative density of 1.10-1.15 (at 50°C). Add sucrose powder and dextrin to the paste, granulate, dry, and make 1500g of granules. Mix thoroughly and package to obtain Wenshen Granules.

[0040] Example 4 Identification (1) Take 6g of Wenshen Granules from Example 1, grind them into powder, add 5ml of ammonia water, soak for 30 minutes, add 30ml of chloroform, heat and reflux for 1 hour, filter, extract the filtrate twice with 5% hydrochloric acid, 20ml each time, combine the acid solution, adjust the pH to 9-12 with ammonia water, extract it again with chloroform twice, 20ml each time, combine the chloroform solution and evaporate it, dissolve the residue in 1ml of anhydrous ethanol to prepare the test solution. Take 1g of Uncaria rhynchophylla as the control medicinal material, and prepare the control medicinal material solution in the same way. According to the thin layer chromatography method (General Rule 0502), take 20μl of the test solution and 5μl of the control medicinal material solution, and spot them on the same silica gel G thin layer plate, use chloroform: ethyl acetate: methanol (10:4:1) as the developing solvent, develop, remove, dry, and examine under ultraviolet light (365nm). In the chromatogram of the test sample, fluorescent spots of the same color appear at the corresponding positions in the chromatogram of the control medicinal material.

[0041] (2) Take 6g of Wenshen Granules from Example 1, grind them into powder, add 20ml of 70% methanol, ultrasonically treat for 30 minutes, filter, evaporate the filtrate to dryness, dissolve the residue in 20ml of water, extract twice with ethyl acetate, combine the extracts, evaporate to dryness, dissolve the residue in 2ml of methanol, and use it as the test solution. Take 2g of Bombyx batryticatus control medicinal material and prepare the control medicinal material solution in the same way. According to the thin layer chromatography method (General Rule 0502), take 5μl of the test solution and 15μl of the control medicinal material solution, and spot them on the same silica gel G thin layer plate. Use ethyl acetate:formic acid:water:toluene (8:1:1:3) as the upper layer solution as the developing solvent, develop, remove, dry, spray aluminum chloride test solution, blow dry with hot air, and examine under ultraviolet light (365nm). In the chromatogram of the test sample, fluorescent spots of the same color appear at the corresponding positions in the chromatogram of the control medicinal material.

[0042] (3) Take 5g of Wenshen Granules from Example 1, grind them into powder, add 30ml of methanol, ultrasonically treat for 30 minutes, filter, evaporate the filtrate to dryness, dissolve the residue in 30ml of water, extract twice with 15ml of water-saturated n-butanol, combine the extracts, evaporate to dryness, dissolve the residue in 2ml of methanol, and use it as the test solution. Separately, take the reference substance of Spinosin, add methanol to make a solution containing 0.01mg per 1ml, and use it as the reference solution. Take 1g of the reference medicinal material of Ziziphus jujuba seeds, and make the reference medicinal material solution in the same way. According to the thin layer chromatography method (General Rule 0502), take 5μl of the test solution, 10μl of the reference medicinal material solution, and 10μl of the reference solution, and spot them on the same silica gel G thin layer plate. Use water-saturated n-butanol:methanol (10:0.5) as the developing solvent, develop the plate, and develop the plate with a developing distance of about 15cm. Take the plate out, dry it, and examine it under ultraviolet light (365nm). In the chromatogram of the test sample, fluorescent spots of the same color appear at the corresponding positions in the chromatograms of the control medicinal materials and the reference sample.

[0043] (4) Take 5g of Wenshen Granules from Example 1, grind them into powder, soak them in 5ml of ammonia water for 5 minutes, then add 25ml of chloroform, reflux them for 1 hour, filter, evaporate the filtrate to dryness, and dissolve the residue in 2ml of methanol to prepare the test solution. Separately, take an appropriate amount of ephedrine reference substance, add methanol to prepare a solution containing 1mg of ephedrine hydrochloride per 1ml, and use this as the reference solution. Take 1g of ephedra reference medicinal material, add 50ml of water and boil for 30 minutes. Evaporate the filtrate to dryness, soak the residue in 5ml of ammonia water, and prepare the reference medicinal material solution in the same manner starting from the "soaking for 5 minutes". According to the thin-layer chromatography method (General Method 0502), 20 μl of the test sample solution, 5 μl of the control herbal solution, and 5 μl of the reference substance solution are spotted separately on the same silica gel G thin-layer plate. Develop with chloroform:methanol:ammonia (20:5:0.5) as the developing solvent. Remove the plate, allow it to air dry, spray with ninhydrin test solution, heat until the spots are clear, and examine under fluorescent light. The test sample chromatogram should show spots of the same color at the corresponding positions on the control herbal and reference substance chromatograms.

[0044] (5) Take 5g of Wenshen Granules from Example 1, grind them into powder, add 30ml of water, ultrasonicate for 30 minutes, centrifuge, take the supernatant, extract twice with ethyl acetate, 20ml each time, combine the extracts, evaporate to dryness, dissolve the residue in 1.5ml of methanol, and use it as the test solution. Take an appropriate amount of β-asarone reference substance, add methanol to make a solution containing 0.2mg of β-asarone per 1ml. Take 2g of Acorus tatarinowii reference medicinal material and prepare the reference medicinal material solution in the same way. According to the thin layer chromatography method (General Rule 0502), take 30μl of the test solution, 5μl of the reference medicinal material solution, and 5μl of the reference solution, and spot them on the same silica gel G thin layer plate. Use toluene:ethyl acetate (3:2) as the developing solvent, develop, remove, dry, spray with 10% sulfuric acid ethanol solution, heat until dry, and examine under ultraviolet light (365nm). In the chromatogram of the test sample, spots of the same color appear at the corresponding positions in the chromatograms of the control medicinal material and the reference sample.

[0045] Example 5 Content Determination: The chromatographic conditions and system suitability test used octadecylsilane bonded silica gel as the filler, acetonitrile as the mobile phase A, and 0.05% phosphoric acid solution as the mobile phase B, and performed gradient elution as specified in the following table; the flow rate was 1.0 ml per minute, the column temperature was 30°C, the detection wavelength was 220 nm, the injection volume was 10 μl, and the theoretical plate number calculated based on the gastrodin peak should be no less than 3000.

[0046] Table 1 Elution gradient

[0047] Preparation of reference solution: Take an appropriate amount of gastrodin reference solution, weigh accurately, and add acetonitrile-water (3:97) mixed solution to prepare a reference solution containing 50 μg of gastrodin per 1 ml.

[0048] Preparation of test solution: Take the Wenshen granules of Example 1, grind them into powder, take about 2 g, accurately weigh, place in a stoppered conical flask, accurately add 50 ml of dilute ethanol, weigh the weight, ultrasonically treat (power 800 W, frequency 40 kHz) for 30 min, let cool, weigh again, make up the lost weight with dilute ethanol, centrifuge at 3000 r / min, centrifuge time 5 min, take the supernatant, concentrate it all to near dryness, dissolve the residue in acetonitrile-water (3:97) mixed solution, transfer to a 25 ml volumetric flask, dilute to the scale with acetonitrile-water (3:97) mixed solution, shake well, filter, and take the filtrate to obtain.

[0049] Determination method: Accurately aspirate 10μl of reference solution and 10μl of test solution, inject into liquid chromatograph, and determine.

[0050] Experimental case: Case record 1, patient Feng Mouhong, male, 10 years old, first visit.

[0051] Chief complaint: tic disorder.

[0052] Medical history: The child had a 2-year history of tics. Current symptoms: blinking, throat clearing, occasional agitation, morning sneezing, good appetite, frequent, unformed bowel movements, and difficulty sleeping.

[0053] Past medical history: tics for more than two years.

[0054] Physical examination: dark red tongue, little coating, red throat, deep pulse.

[0055] Prescription: 1. 10 packs of the anti-spasm granules of Example 1. 2 bags each time, 2 times a day.

[0056] Follow-up visit: Symptoms have been alleviated, but the patient is easily nervous. No obvious symptoms are seen now, and treatment is being continued.

[0057] 2. The patient is Gao, a 10-year-old girl, who is on her first visit.

[0058] Chief complaint: throat clearing and blinking for two months.

[0059] Medical history: Child has had throat clearing and blinking for two months. Bowel movements are now bi-daily, dry, with mild rhinitis and runny nose. Sleep is good. Diet is moderate.

[0060] Past history: conjunctivitis three months ago; elevated myocardial enzymes in September 2021.

[0061] Physical examination: dark red tongue, little coating, red throat, deep pulse.

[0062] Prescription: 10 pairs of the anti-spasm granules of Example 1. 2 bags each time, 2 times a day.

[0063] Follow-up visit: Current symptoms: Blinking has improved, but nodding and shaking symptoms persist, and worsen after using electronic devices. Recently, there has been nasal congestion and shortness of breath. Occasional urticaria. Bowel movements are normal, appetite is poor, and sleep is normal.

[0064] 3. The patient is Li Mouyang, male, 17 years old, first visit.

[0065] Chief complaint: blinking and leg shaking for two months.

[0066] Current medical history: Blinking, leg shaking, frequent small movements, poor concentration, and irritability. Bowel movements and bowel movements are normal, and appetite and sleep are good. Currently taking 7 boxes of atomoxetine per week.

[0067] Physical examination: red tongue with scanty coating, red throat, deep pulse. EEG normal.

[0068] Western medicine diagnosis: tic disorder.

[0069] Prescription: 10 packets of the anti-spasm granules of Example 1. 2 bags each time, twice a day.

[0070] Follow-up visit: Symptoms have been alleviated. Concentration remains difficult. Bowel movements are normal, and appetite and sleep are good.

[0071] 4. The patient, Li Mouxi, is a 10-year-old boy and is on the first visit.

[0072] Chief complaint: nodding, throat clearing, blinking, and staring for two months.

[0073] Current medical history: Two months ago, nodding, throat clearing, blinking, and glaring developed without apparent cause. Improvement was seen after self-administration of calcium and zinc. Symptoms worsened 10 days ago. Improvement was seen after self-administration of Tianma Gou Teng Granules. Susceptibility to colds and poor concentration are common. Appetite is slightly poor and the patient is a picky eater. Sleep is acceptable. Excessive sweating. Slightly dry bowel movements occur every two to three days, and urination is normal.

[0074] Physical examination: red tongue, little coating, red throat, deep pulse.

[0075] Prescription: 14 packets of the anti-spasmodic granules of Example 1. Take one packet each morning and evening.

[0076] Follow-up visit: Throat clearing symptoms have eased, but shoulder shrugging is still present. Sweating has improved. Appetite and sleep are good, and bowel movements are normal. Easily startled.

[0077] Physical examination: red tongue, little coating, red throat, deep pulse.

[0078] 5. The patient is Li Mouao, male, 7 years old, first visit.

[0079] Chief complaint: Tics for more than three months.

[0080] Current medical history: Diagnosed with "tic disorder" at Shengjing Hospital one month ago. Current symptoms: blinking, teeth grinding, shoulder shrugging, and twitching of the corners of the mouth, which persists during sleep at night. Also, the patient bites clothing and fingers. Eczema on the thighs. Appetite is good. Sleep is slightly worse. Bowel movements are normal.

[0081] Physical examination: dark red tongue, scanty coating, deep pulse; normal EEG; normal levels of various trace elements.

[0082] Diagnosis: Tic disorder.

[0083] Prescription: 10 packets of the anti-spasm granules of Example 1. 2 bags each time, twice a day.

[0084] Follow-up visit: Tic symptoms improved, but eczema persists. Nasturtium and sleepiness are normal, bowel movements are normal.

[0085] 6. The patient is Liu Mouyi, male, 13 years old, first visit.

[0086] Chief complaint: Allergic rhinitis. Throat clearing and nasal sniffing for over 2 months.

[0087] Present medical history: occasional sneezing, normal appetite and sleep, and normal bowel movements.

[0088] Past medical history: adenoidectomy 2 years ago; hospitalized for pneumonia half a year ago.

[0089] Physical examination: dark red tongue, little coating, deep pulse.

[0090] Prescription: Decoction of Example 1, 7 doses. Decoction in water, one dose per day, divided into two doses to be taken warm.

[0091] Follow-up visit: Clearing the throat, blowing the nose and other small movements occur occasionally, especially in the morning, before going to bed and when exposed to cold.

[0092] 7. The patient is Liu Mouxi, female, 11 years old, first visit.

[0093] Chief complaint: Involuntary shaking of the head and blinking for 3 months.

[0094] Current medical history: Involuntary shaking and blinking of the head for 3 months. Appetite and sleep are normal, bowel movements and bowel movements are regular.

[0095] Physical examination: pale red tongue, white fur, deep pulse.

[0096] Prescription: 10 packets of the anti-spasm granules of Example 1. 2 bags each time, twice a day.

[0097] Follow-up visit: Shaking head and blinking. Symptoms gradually relieved, once every 2 minutes. Namei Ke, bowel movements normal.

[0098] 8. The patient is Liu Mouen, male, 13 years old, first visit.

[0099] Chief complaint: tic disorder.

[0100] Current medical history: Two febrile seizures last year. On February 20th of this year, a seizure occurred without cause. Diagnosed with "tic disorder" at a medical university. Occasional facial twitching. A large appetite. Bad breath. Nose. Appetite and sleep are normal. Sleeps on the stomach. Bowel movements are regular.

[0101] Physical examination: red tongue, scanty coating, deep pulse.

[0102] Prescription: 10 packets of the anti-spasm granules of Example 1. 2 bags each time, twice a day.

[0103] Follow-up visit: Blinking and sniffling have improved. Emotional agitation and excessive sweating.

[0104] 9. The patient is Liu Mouhao, male, 6 years old, first visit.

[0105] Chief complaint: Tic disorder for more than one month.

[0106] Current medical history: blinking, slight twitching of the corners of the mouth and eyes when lowering the head, frequent nodding and clearing of the throat.

[0107] Physical examination: pale tongue, scanty coating, deep pulse.

[0108] Prescription: 10 packets of the anti-spasm granules of Example 1. 2 bags each time, twice a day.

[0109] Follow-up visit: Blinking and shrugging gradually improved. Poor appetite, craving meat. Frequent dreams. Dry stool. Urination is regular every 2-3 days.

[0110] 10. The patient is Ma Mouyi, female, 6 years old, first visit.

[0111] Chief complaint: facial twitching, blinking, and throat clearing.

[0112] Present medical history: facial twitching, blinking, throat clearing. Appetite and sleep are good, bowel movements are normal.

[0113] Physical examination: pale tongue, scanty coating, deep pulse.

[0114] Prescription: 10 packets of the anti-spasm granules of Example 1. 2 bags each time, twice a day.

[0115] Follow-up visit: facial twitching, normal appetite and sleep, regular bowel movements.

[0116] 11. The patient is Mi Mouxi, female, 6 years old, first visit.

[0117] Chief complaint: facial twitching.

[0118] Present medical history: Occasional facial twitching, blinking, sniffling, and twitching of the corners of the mouth. Abdominal distension, good appetite, and loose or dry stools, occurring once every 1-2 days.

[0119] Physical examination: red tongue, scanty coating, deep pulse.

[0120] Prescription: 10 packets of the anti-spasm granules of Example 1. 2 bags each time, twice a day.

[0121] 12. The patient is Miao Mourong, male, 11 years old, first visit.

[0122] Chief complaint: jaw twitching for 1 month.

[0123] Current medical history: blinking, prone to oral ulcers, poor appetite, good sleep, and normal bowel movements.

[0124] Past medical history: allergic rhinitis.

[0125] Physical examination: pale tongue, scanty coating, deep pulse.

[0126] Prescription: 10 packets of the anti-spasm granules of Example 1. 2 bags each time, twice a day.

[0127] 13. The patient is Qin Mouhan, male, 9 years old, first visit.

[0128] Chief complaint: Facial tics for 2 years, aggravated for 2 months.

[0129] Current medical history: Facial tics for 2 years, worsening for 2 months. Symptoms worsen with stress. Two weeks ago, I developed a cold, cough, and runny nose. My appetite is poor, but I sleep well. My bowel movements are normal, with bowel movements every 3 days.

[0130] Past medical history: allergic rhinitis.

[0131] Physical examination: red tongue, little coating, red throat, deep pulse.

[0132] Prescription: 1. 10 packets of the Zhichu Granules of Example 1. 2 bags each time, twice a day. 2. 4 boxes of Kaihoujian.

[0133] Follow-up visit on November 9, 2022: Symptoms remain the same as before.

[0134] 14. The patient is Ren Mouze, a 10-year-old boy, first visit.

[0135] Chief complaint: tics.

[0136] Current medical history: Occasional blinking, lasting 2 months. Self-administered Chinese medicine for 6 weeks. Involuntary hand movements, foot stamping, and teeth clenching. Sleeping is normal, with frequent dreams, and bowel movements are normal.

[0137] Physical examination: red tongue, little coating, red throat, deep pulse.

[0138] Prescription: 1. Take 10 packets of the anti-spasm granules of Example 1, 2 bags each time, twice a day.

[0139] 15. The patient is Shi Mourui, a 5-year-old boy, first visit.

[0140] Chief complaint: Frequent neck twitching for 2 months.

[0141] Current medical history: Symptoms worsen when turning the head or looking at electronic devices. Occasional blinking and timidity. Appetite and sleep are normal, and bowel movements are regular.

[0142] Physical examination: red tongue with scanty coating, deep pulse, normal EEG.

[0143] Prescription: 10 packets of the anti-spasm granules of Example 1. 2 bags each time, twice a day.

[0144] Follow-up visit: The condition has improved in recent days. Appetite and sleep are good, bowel movements are normal.

[0145] 16. The patient, Song Mouzhe, male, 8 years old, first visit.

[0146] Chief complaint: Tic disorder for more than one month.

[0147] Current medical history: The child developed nodding symptoms a month ago. Later, he developed blinking and nasal sniffling. He was referred to a medical institution, where his symptoms improved. He is now asymptomatic. He has a poor appetite, dry, coarse stools, and frequent bowel movements every three to four days. He frequently sweats after sleeping.

[0148] Physical examination: pale red tongue with scanty coating and deep pulse. External hospital EEG: borderline pediatric EEG.

[0149] Diagnosis: Tic disorder.

[0150] Prescription: 1. 10 packets of the anti-spasmodic granules from Example 1. Take 2 packets each time, twice daily. 2. 7g of Scrophulariae, 7g of Amomum villosum, and 15g of Honeysuckle. Take 14 doses of the granules with water, one packet each time, twice daily.

[0151] 17. The patient, Sun Mouxin, is an 11-year-old boy and is at the first visit.

[0152] Chief complaint: abdominal suction and involuntary sounds in the throat for more than 2 months.

[0153] Current medical history: The child developed blinking and involuntary vocalizations in the throat at 1 year old. He was subsequently referred to the Second Hospital of Medical University and diagnosed with tic disorder. Symptoms have recurred over the past year. Two months ago, he developed abdominal sucking and involuntary vocalizations, which were more severe at bedtime. His appetite, bowel movements, and sleep were normal, with occasional dizziness.

[0154] Physical examination: pale red tongue, scanty coating, deep pulse.

[0155] Prescription: 1. Take 10 packets of the "Zhi Chuan Granules" from Example 1. Take 2 packets each time, twice daily. 2. Take 7g of Acorus calamus, 7g of Amomum villosum, 20g of Honeysuckle, 10g of White Peony, 10g of Corydalis, 6g of Magnolia officinalis. Take 14 doses of the granules with water, one packet each time, twice daily.

[0156] Follow-up visit: Symptoms improved, with occasional facial twitching, involuntary vocalizations in the throat, and abdominal aspiration. Appetite and sleep are normal. Bowel movements are normal.

[0157] 18. The patient, Mr. Sun, is a 10-year-old boy and is at the first visit.

[0158] Chief complaint: Involuntary shaking of head for more than half a month.

[0159] Current medical history: The child has been shaking his head involuntarily for over half a month. Symptoms have improved with relaxation. Nasal congestion and runny nose are present. Appetite and sleep are normal, and bowel movements are normal.

[0160] Physical examination: red tongue, little coating, deep pulse.

[0161] Prescription: 10 packets of the anti-spasm granules of Example 1. 2 bags each time, twice a day.

[0162] 19. The patient is Sun Moufu, female, 6 years old, first visit.

[0163] Chief complaint: Shrugging shoulders for more than half a month.

[0164] Current medical history: The child's parents noticed frequent shrugging of the shoulders two weeks ago, with a history of fright. Symptoms were absent when the child was stressed, but recurred when the child relaxed. Currently, the child blinks, clears the throat, and sniffles. The child was diagnosed with rhinitis at a local hospital. His appetite and sleep have improved, and his bowel movements have been normal.

[0165] Physical examination: red tongue, little coating, deep pulse.

[0166] Past medical history: He was diagnosed with rhinitis at a local hospital.

[0167] Prescription: 10 pairs of the anti-spasm granules of Example 1. 2 bags each time, 2 times a day.

[0168] Follow-up visit: Symptoms have improved recently, with occasional stomach twitching, sniffling, and involuntary vocalizations. Appetite is good, bowel movements are normal, and sleep is slightly better.

[0169] 20. The patient is Wang Han, a 13-year-old boy, first visit.

[0170] Chief complaint: Tic disorder for more than 10 years.

[0171] Current medical history: The child had previously taken medication at our clinic but has stopped for six months. Symptoms recurred three days ago following a vaccination. Currently, the child blows his nose and trembles as if in a state of panic. He also has unusual movements. He has a strong appetite, dry stools, only one per day, and frequent urination.

[0172] Physical examination: pale red tongue, scanty coating, deep pulse.

[0173] Prescription: 10 packets of the anti-spasm granules of Example 1. 2 bags each time, twice a day.

[0174] Follow-up visit: Nasal blowing has improved recently, but hand movements persist. Appetite is good, sleep is slightly poor, and falling asleep is difficult. Stools are loose, three times a day.

[0175] 21. The patient is Wang Mouran, a 13-year-old boy, first visit.

[0176] Chief complaint: tic disorder.

[0177] Current medical history: The patient previously took medication at our clinic, with some relief. She now requests continued treatment. Current symptoms: Appetite is good, but she is restless at night.

[0178] Physical examination: pale red tongue, scanty coating, deep pulse.

[0179] Prescription: 10 packets of the anti-spasm granules of Example 1. 2 bags each time, twice a day.

[0180] 22. The patient is Wang Moutao, male, 6 years old, first visit.

[0181] Chief complaint: Hyperactivity for more than 2 months.

[0182] Present medical history: The child has an irritable temper and difficulty concentrating. Concentration tests indicate poor attention span. He sweats profusely, is prone to allergies, and suffers from prickly heat. His appetite and sleep are normal, and his bowel movements are regular.

[0183] Past medical history: urticaria Physical examination: pale red tongue, scanty coating, deep pulse.

[0184] Prescription: 10 packets of the Zhichu Granules from Example 1. Take 2 bags each time, twice daily. Follow-up: Symptoms have improved recently, and the patient's temper has gradually improved. Current symptoms: Hyperactivity, poor concentration, good sleep, and regular bowel movements.

[0185] 23. The patient is Wang Su, female, 7 years old, first visit.

[0186] Chief complaint: shaking head and blinking for more than 8 months, aggravated for 1 month.

[0187] Current medical history: After the Spring Festival this year, the child was found shaking his head. He was taken to Shengjing Hospital and diagnosed with Tourette syndrome. While treatment has not yet begun, the symptoms have resolved. One month ago, the shaking worsened, accompanied by blinking. He has been receiving pediatric massage for one month. His appetite and sleep are normal, and his bowel movements are normal.

[0188] Past medical history: egg allergy.

[0189] Physical examination: pale red tongue with scant coating, deep pulse. Normal pediatric EEG topography. Normal intracranial arterial blood flow velocity.

[0190] Diagnosis: Tic disorder.

[0191] Prescription: 10 packets of the anti-spasm granules of Example 1. 2 bags each time, twice a day.

[0192] Follow-up visit: Symptoms were less pronounced recently, but now: Head symptoms have eased, but blinking, shrugging, and shaking of the head persist, especially during periods of emotional excitement. Sleep is good, and bowel movements are normal.

[0193] 24. The patient is Wang Mouhang, male, 9 years old, first visit.

[0194] Chief complaint: Facial twitching and frequent hiccups for more than a month.

[0195] Present medical history: The child has had facial twitching and frequent hiccups for over a month. He also experiences discomfort in his throat, as if a foreign object is present. He is easily startled and palpitates. Self-administration of calcium, iron, and zinc has not alleviated his symptoms. His appetite and sleep are normal, and his bowel movements are regular.

[0196] Past medical history: urticaria Physical examination: pale red tongue with scanty coating and deep pulse. EEG topography of a normal child.

[0197] Diagnosis: Tic disorder.

[0198] Prescription: 10 packets of the anti-spasm granules of Example 1. 2 bags each time, twice a day.

[0199] 25. The patient is Wang Moufan, male, 9 years old, first visit.

[0200] Chief complaint: Tic disorder diagnosed more than 1 month ago.

[0201] Present medical history: The child's parents reported neck twitching and involuntary vocalizations for over two years, which worsened over the past month. The child was taken to the Second Hospital of Medical University and diagnosed with Tourette Syndrome. His appetite and sleep were normal, and his bowel movements were normal.

[0202] Physical examination: pale red tongue, scanty coating, deep pulse.

[0203] Prescription: 1. 10 packets of the anti-spasm granules from Example 1. Take 2 packets each time, twice daily. 2. 20g of honeysuckle and 7g of peppermint. Take 14 doses of granules with water, one packet each time, twice daily.

[0204] Follow-up visit: Symptoms improved after taking the medicine, but no significant improvement has been observed in the past week. Sleep is good, and bowel movements are normal.

[0205] Physical examination: red tongue, little coating, deep pulse.

[0206] 26. The patient is Wang Moujun, male, 11 years old, first visit.

[0207] Chief complaint: Tic for more than half a year.

[0208] Current medical history: Six months ago, the child developed groaning and throat clearing. He has had involuntary hip twitching for the past week. His appetite and sleep are normal, and his bowel movements are regular.

[0209] Physical examination: pale red tongue, scanty coating, deep pulse; normal pediatric EEG topography; normal trace elements.

[0210] Diagnosis: Tic disorder.

[0211] Prescription: 10 packets of the anti-spasm granules of Example 1. 2 bags each time, twice a day.

[0212] 27. The patient is Wang Mouxiao, male, 9 years old, first visit.

[0213] Chief complaint: Neck twitching for more than 3 months.

[0214] Current medical history: The patient developed tics three years ago, first in the head and then in the hands. He also had poor sleep and frequently tossed and turned during sleep. He was a picky eater. His bowel movements were normal.

[0215] Physical examination: Pale red tongue with scant coating, deep pulse. Normal pediatric EEG topography. Total 25-hydroxyvitamin D: 19.080 ng / ml.

[0216] Diagnosis: Tic disorder.

[0217] Prescription: 1. Take 10 packets of the Zhichu Granules of Example 1. Take 2 bags each time, twice a day. 28. Patient Wen Mouxuan, female, 3 years old, first visit.

[0218] Chief complaint: blinking and blowing nose for more than half a year.

[0219] Current medical history: The child had been blinking and blowing his nose for over six months, with occasional limb and neck twitching. He was picky about food, tossed and turned during sleep, and had irregular bowel movements with dry stools.

[0220] Past medical history: adenoid hypertrophy.

[0221] Physical examination: pale red tongue, scanty coating, deep pulse.

[0222] Prescription: 10 packets of the anti-spasm granules of Example 1. 2 bags each time, twice a day.

[0223] Follow-up visit: The child's symptoms have improved. He now has nasal blowing, which is more pronounced when concentrating or when emotionally agitated. He also has nasal congestion with white discharge and nail biting. His appetite and sleep are good, he prefers to sleep on his stomach, and his bowel movements are normal.

[0224] 30. The patient is Wu Mouchen, male, 4 years old, first visit.

[0225] Chief complaint: blinking and twitching of mouth corners for more than half a month.

[0226] Present medical history: The child has been blinking, twitching at the corners of his mouth, and clearing his throat for half a month. He visited a children's hospital three days ago and took Changma Xifeng tablets for three days, but there was no significant improvement. The child is prone to colds. His appetite is good, his sleep is normal, and his bowel movements are regular.

[0227] Past medical history: adenoidectomy. Allergic constitution.

[0228] Physical examination: red tongue, scanty coating, deep pulse.

[0229] Prescription: 10 packets of the anti-spasm granules of Example 1. 2 bags each time, twice a day.

[0230] Follow-up visit: Current findings: Nasal blowing has eased, blinking has improved. The child is usually irritable, likes to pull at clothes, has poor sleep, and frequently tosses and turns during sleep. He is a picky eater, but his bowel movements are normal.

[0231] 31. The patient, Xu Mouchen, male, 9 years old, first visit.

[0232] Chief complaint: Tics for more than 2 months.

[0233] Present medical history: The child has been blinking, blowing his nose, and clearing his throat for two months. Symptoms are more pronounced after activities, with head twisting. He usually has normal attention span and excellent grades, but today he has become irritable. He is moderately obese. His appetite and sleep are normal, and his bowel movements are regular.

[0234] Physical examination: pale red tongue, thin white fur, deep pulse.

[0235] Prescription: 10 packets of the anti-spasm granules of Example 1. 2 bags each time, twice a day.

[0236] Follow-up visit: Current findings: Blinking symptoms persist, but other symptoms have eased, with blinking symptoms gradually improving. Mood is gradually stabilizing. Appetite and sleep are good, and bowel movements are normal.

[0237] 32. The patient is Xu, a 9-year-old boy, first visit.

[0238] Chief complaint: Tics for more than 3 years.

[0239] Current medical history: The child's blinking symptoms worsened during the Spring Festival due to dietary restrictions. Western medical treatment with brain protein and Jingling oral liquids for 10 months has not shown significant improvement. His appetite and sleep are normal, and his bowel movements are normal.

[0240] Past medical history: tonsillectomy and adenoidectomy 3 years ago. Allergic rhinitis.

[0241] Physical examination: red tongue, white fur, deep pulse.

[0242] Prescription: 10 packets of the anti-spasm granules of Example 1. 2 bags each time, twice a day.

[0243] 33. The patient, Yin Chang, is a 4-year-old boy and is on the first visit.

[0244] Chief complaint: blinking for more than 2 months.

[0245] Current medical history: The child has been blinking for over 2 months. He has profuse, sticky mucus. His appetite and sleep are normal, and his bowel movements are regular.

[0246] Physical examination: red tongue, white fur, deep pulse.

[0247] Prescription: 10 packets of the anti-spasm granules of Example 1. 2 bags each time, twice a day.

[0248] 34. The patient is Zhang Rui, a 7-year-old boy, first visit.

[0249] Chief complaint: Throat discomfort for more than half a month.

[0250] Current medical history: The child developed abnormal laryngeal vocalizations in May of this year, with high-pitched sounds, particularly after activity. His appetite and sleep were normal, and his bowel movements were regular.

[0251] Physical examination: red tongue, little coating, deep pulse.

[0252] Past medical history: pharyngitis.

[0253] Prescription: 10 packets of the anti-spasm granules of Example 1. 2 bags each time, twice a day.

[0254] Follow-up visit: The child's symptoms have slightly improved. Two days ago, the child developed a cold and fever. He self-treated with Motrin twice and 1.5 bags of Xiaoer Chaigui Granules. Throat discomfort and a high-pitched voice have worsened. Appetite and sleep are good, and bowel movements are normal.

[0255] 35. The patient is Zhang Ming, a 10-year-old boy, first visit.

[0256] Chief complaint: tic disorder.

[0257] Current medical history: The child has a tic disorder and is often irritable and irritable. He has dry eyes. His appetite and sleep are normal, and his bowel movements are regular, but his stools are dry.

[0258] Physical examination: red tongue, little coating, deep pulse.

[0259] Prescription: 10 packets of the anti-spasm granules of Example 1. 2 bags each time, twice a day.

[0260] 36. The patient is Zhang Mouxuan, female, 7 years old, first visit.

[0261] Chief complaint: intermittent deep inspiration for 2 weeks.

[0262] Current medical history: The patient had intermittent deep breathing for two weeks without apparent cause, and abdominal cramps for the past three days. Sleep was poor, with open-mouth breathing and frequent teeth grinding. Occasional coughing was noted. Appetite was good, and bowel movements were regular, but stools were dry.

[0263] Physical examination: red tongue, scanty coating, deep pulse.

[0264] Prescription: 10 packets of the anti-spasm granules of Example 1. 2 bags each time, twice a day.

[0265] 37. The patient, Zhang Mouze, is an 11-year-old boy and is on the first visit.

[0266] Chief complaint: blinking for more than 2 months.

[0267] Current medical history: The child has blinked and pursed his lips for over two months. His concentration and academic performance have declined. He occasionally experiences throat itching and throat clearing. He frequently uses electronic devices. He has taken Namib and has loose stools, with one stool every three to four days.

[0268] Physical examination: pale red tongue, scanty coating, deep pulse.

[0269] Prescription: 10 packets of the anti-spasm granules of Example 1. 2 bags each time, twice a day.

[0270] Follow-up visit: The patient still has facial tics and blinks occasionally. His appetite is good and his stools are loose, occurring once every four days.

[0271] 38. Patient Zhao Huabo, female, 14 years old, first visit.

[0272] Chief complaint: throat clearing for more than 5 years.

[0273] Present medical history: The child clears his throat, blinks, pouts, and rubs his nose. He sleeps well, breathing through his mouth while asleep. He is a picky eater. His bowel movements are regular, but his stools are dry.

[0274] Physical examination: red tongue, scanty coating, deep pulse.

[0275] Prescription: 10 packets of the anti-spasm granules of Example 1. 2 bags each time, twice a day.

[0276] 39. The patient, Zhao Mouyang, male, 8 years old, first visit.

[0277] Chief complaint: blinking, blowing nose, and clearing throat for more than 4 months, aggravated for one month.

[0278] Current medical history: The child has been blinking, blowing his nose, and clearing his throat for over four months, with symptoms worsening over the past month. He has a poor appetite and is a picky eater. He is often irritable. His stools are dry and his urine is regular.

[0279] Past medical history: allergic conjunctivitis and rhinitis for 3 years.

[0280] Physical examination: red tongue, scanty coating, deep pulse, and pharyngeal congestion (+).

[0281] Prescription: 10 packets of the anti-spasm granules of Example 1. 2 bags each time, twice a day.

[0282] Follow-up visit: The child's blinking symptoms have not improved significantly. Namei is good, and the stool is formed, with one bowel movement every 1-2 days.

[0283] 40. Patient Zhao Zhixuan, female, 9 years old, first visit.

[0284] Chief complaint: Twitching at the corners of mouth for 1 year.

[0285] Current medical history: The patient has had twitching at the corners of his mouth for a year and frequent enuresis. Six months ago, abdominal twitching developed after pediatric massage therapy. His appetite and sleep are normal, and his bowel movements are regular.

[0286] Physical examination: pale red tongue, scanty coating, deep pulse.

[0287] Prescription: 10 packets of the anti-spasm granules of Example 1. 2 bags each time, twice a day.

[0288] Follow-up visit: The patient's facial tics were significantly improved. His appetite and sleep were good, his nocturia was frequent, and his bowel movements were regular.

[0289] 41. The patient, Zhu Moulong, is an 11-year-old boy and is at the first visit.

[0290] Chief complaint: throat clearing and blinking for more than 4 years.

[0291] Present medical history: The patient has been experiencing throat clearing and blinking for over 4 years. He also has occasional twitching of his limbs and shrugging of his shoulders. His appetite and sleep are normal, and his bowel movements are regular.

[0292] Past medical history: adenoidectomy.

[0293] Physical examination: red tongue, white fur, deep pulse.

[0294] Prescription: 10 packets of the anti-spasm granules of Example 1. 2 bags each time, twice a day.

[0295] Follow-up visit: The child frequently cleared his throat and shrugged his shoulders. His nasal blowing symptoms had improved. His appetite and sleep were good, and his bowel movements were normal.

[0296] The foregoing description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Those skilled in the art will readily appreciate that various modifications and variations of the present invention are possible. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present invention are intended to be within the scope of protection of the present invention.

Claims

1. A stabilizing composition, characterized in that: The invention is composed of the following raw materials in parts by weight: 5-10 parts of Gastrodia elata, 5-10 parts of Bombyx batryticatus, 3-6 parts of Uncaria rhynchophylla, 2-4 parts of Ephedra, 5-10 parts of stir-fried Ziziphus jujuba seeds, 2-4 parts of Radix dahliae, 10-20 parts of Poria cocos and 3-6 parts of Acorus calamus.

2. The sedative composition according to claim 1, wherein The invention is composed of the following raw materials in parts by weight: 5-7.5 parts of Gastrodia elata, 5-8 parts of Bombyx batryticatus, 3-4.5 parts of Uncaria rhynchophylla, 2-3 parts of Ephedra sinica, 5-7.5 parts of stir-fried Ziziphus jujuba seeds, 2-3 parts of Radix dahliae, 10-15 parts of Poria cocos and 3-4.5 parts of Acorus calamus.

3. The sedative composition according to claim 1, wherein The invention is composed of the following raw materials in parts by weight: 7.5-10 parts of Gastrodia elata, 7.5-10 parts of Bombyx batryticatus, 4.5-6 parts of Uncaria rhynchophylla, 3-4 parts of Ephedra, 7.5-10 parts of stir-fried Semen Ziziphi spinulosae, 3-4 parts of Radix Glehniae, 15-20 parts of Poria cocos and 4.5-6 parts of Acorus calamus.

4. A method for preparing a stabilizing composition, characterized in that: include: Mix Gastrodia elata, Bombyx batryticatus, Uncaria rhynchophylla, Ephedra sinica, stir-fried Ziziphus jujuba seeds, Rhizoma jasminoides, Poria cocos and Acorus calamus evenly, add water and boil to obtain the medicine.

5. A traditional Chinese medicine preparation for stabilizing the mind, characterized in that: include: The sedative composition according to any one of claims 1 to 3.

6. The traditional Chinese medicine preparation for calming the mind as claimed in claim 5, characterized in that The dosage form is selected from at least one of decoction, pill, powder, ointment, lotion, pill, wine, and tablet.

7. A quality control method for a stabilizing composition, characterized in that: include: The corresponding test sample solutions were prepared with Uncaria rhynchophylla, Bombyx batryticatus, Semen Ziziphi spinulosae, Ephedra sinica and Acorus tatarinowii as control medicinal materials respectively, and the products were identified by thin layer chromatography.

8. The quality control method of the Shen-stable composition according to claim 7, characterized in that: When using jujube seeds as the reference medicinal material, spinosin was used to prepare the reference solution; Alternatively, when using ephedra as the reference herb, use ephedrine hydrochloride to prepare the reference solution; When Acorus tatarinowii was used as the reference medicinal material, β-asarone was used to prepare the reference solution.

9. A method for determining the content of a stabilizing composition, characterized in that: Octadecylsilane bonded silica gel was used as the filler, acetonitrile was used as the mobile phase A, and 0.05% phosphoric acid solution was used as the mobile phase B for gradient elution. The conditions for gradient elution are: 0-15 min, mobile phase A 2%; 15-17 min, mobile phase A 2%-15%; 17-25 min, mobile phase A 15%-70%; 25-25.01 min, mobile phase A 70%-2%; 25.01-35 min, mobile phase A 2%.

10. The method for determining the content of the Shen-sense composition according to claim 9, wherein: The flow rate is 1.0 ml per minute; Alternatively, the column temperature was 30°C; Alternatively, the detection wavelength is 220 nm; Alternatively, the injection volume is 10 μl.

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