Accurate culture device for MANF gene modified umbilical cord mesenchymal stem cells

Abstract

The invention belongs to the field of culture equipment, and particularly relates to an accurate culture device for MANF gene modified umbilical cord mesenchymal stem cells, which comprises a culture box, a box cover is arranged at the top of the culture box, an observation window is arranged at the top of the box cover, and a plurality of support rods are fixedly mounted on the inner wall of the bottom of the culture box. The top ends of the multiple supporting rods are fixedly provided with the same placing table, a culture dish is placed at the top of the placing table, and a clamping mechanism is arranged between the placing table and the culture dish. The device is reasonable in design, the culture dish is stably clamped through the push rod motor, the temperature and oxygen concentration sensor is matched with the heating piece, the refrigeration piece and the electromagnetic valve to precisely control temperature and adjust oxygen, and the driving motor drives the microscopic camera to rotate to collect data to achieve real-time monitoring; the microscopic camera is moved by virtue of cooperation of the vertical plate, the lifting plate and the spiral plate, so that stem cell images are comprehensively acquired, stable growth of stem cells is guaranteed, and a suitable environment is provided for each growth stage.

Description

Technical Field

[0001] The present invention relates to the technical field of culture equipment, and in particular to a precise culture device for MANF gene-modified umbilical cord mesenchymal stem cells. Background Art

[0002] Umbilical cord mesenchymal stem cells (UMBS) possess the potential for self-renewal and multidirectional differentiation, demonstrating enormous application potential in regenerative medicine and tissue engineering. Cultivating MANF-modified UMBS requires strict control of parameters such as temperature and oxygen concentration, while also enabling real-time monitoring and analysis of cell growth.

[0003] Currently, existing stem cell culture devices lack the precision to control environmental parameters and the comprehensiveness to monitor cell growth, making them difficult to meet the requirements for the precise culture of MANF gene-modified umbilical cord mesenchymal stem cells. For example, some culture devices are unable to fully capture images of stem cells within the culture dish, resulting in an inability to accurately and timely monitor cell growth. Furthermore, untimely and inaccurate adjustments to the culture environment can affect the quality and efficiency of stem cell culture. Therefore, we have proposed a precise culture device for MANF gene-modified umbilical cord mesenchymal stem cells to address these issues. Summary of the Invention

[0004] The purpose of the present invention is to address the shortcomings of the prior art and to propose a precise culture device for MANF gene-modified umbilical cord mesenchymal stem cells.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] A precise culture device for MANF gene-modified umbilical cord mesenchymal stem cells comprises an incubator, the incubator having a lid on top, an observation window on top, a plurality of support rods fixedly mounted on the bottom inner wall of the incubator, a common placement table fixedly mounted on the top ends of the plurality of support rods, a culture dish placed on top of the placement table, and a clamping mechanism provided between the placement table and the culture dish; the incubator having a chamber, a heating plate mounted on the inner wall of the chamber, a cooling plate mounted on the bottom inner wall of the chamber, a temperature sensor and an oxygen concentration sensor mounted on one inner wall of the incubator, a gas cylinder mounted on one side of the incubator, an air outlet pipe mounted on the bottom of the gas cylinder, a mixing box mounted on the bottom of the air outlet pipe, an air inlet pipe connected to the incubator mounted on one side of the mixing box, and solenoid valves provided on both the air outlet pipe and the air inlet pipe;

[0007] The clamping mechanism includes a movable groove, a clamping seat and a fixed spring. The top of the placement table is provided with a plurality of movable grooves in a circular shape with equal intervals. A clamping seat for fixing the culture dish is slidably installed in the movable groove. A fixed spring is fixedly installed on one side of the clamping seat. One end of the fixed spring is fixedly installed on the inner wall of the movable groove. An annular plate is fixedly installed on the bottom of the placement table. An annular rotating plate rotatably connected to the placement table is rotatably installed on the top of the annular plate. A vertical plate is fixedly installed on the top of the annular rotating plate. A cross bar is fixedly installed on the top of the vertical plate. A sliding seat is slidably sleeved on the cross bar, and a microscope camera for image acquisition is fixedly installed on the sliding seat.

[0008] Preferably, a push rod motor is fixedly mounted on the bottom of the placement table, a driving plate is fixedly mounted on the output shaft of the push rod motor, and a pulling mechanism is provided between the driving plate and the clamping seat.

[0009] Preferably, the pulling mechanism includes a guide groove, a wire rod and a connecting rope. A guide groove is opened on the inner wall of one side of the movable groove, and wire rods are fixedly installed on the inner walls on both sides of the guide groove. A connecting rope is fixedly installed on one side of the clamping seat, and one end of the connecting rope is wrapped around the wire rod and fixedly installed on the driving plate.

[0010] Preferably, limiting grooves are provided on both inner walls of the movable groove, limiting seats are fixedly installed on both sides of the clamping seat, and the limiting seats are slidably connected to the corresponding limiting grooves.

[0011] Preferably, an annular rack is installed on the outer side of the annular rotating plate, a mounting bracket is installed on the bottom inner wall of the incubator, a driving motor is fixedly installed on the top of the mounting bracket, a driving gear is fixedly installed on the output shaft of the driving motor, and the driving gear is meshed with the annular rack.

[0012] Preferably, an annular slide groove is provided on the outer side of the placement table, an annular slide seat is fixedly mounted on the inner wall of the annular rotating plate, and the annular slide seat is rotatably connected to the annular slide groove.

[0013] Preferably, an annular fixing plate is fixedly installed on the top of the placement table, a spiral plate is fixedly installed on the outside of the annular fixing plate, a lifting groove is opened on one side of the vertical plate, a lifting plate is slidably installed in the lifting groove, and the lifting plate is slidably installed between the spiral plates.

[0014] Preferably, two connecting seats are fixedly installed on one side of the vertical plate, a rotating gear is rotatably installed between the two connecting seats, and a transmission mechanism is provided between the lifting plate and the microscope camera.

[0015] Preferably, the transmission mechanism includes a driving seat, rack 1, a pushing seat and rack 2, the driving seat is fixedly mounted on the top of the lifting plate, rack 1 is fixedly mounted on one side of the driving seat, the pushing seat is fixedly mounted on one side of the microscope camera, rack 2 is fixedly mounted on the top of the pushing seat, and rack 1 and rack 2 are both engaged with the rotating gear.

[0016] Preferably, a controller is installed on the top of the box cover, and the temperature sensor, oxygen concentration sensor, push rod motor, drive motor and solenoid valve are all electrically connected to the controller.

[0017] Beneficial effects of the present invention:

[0018] 1. To ensure the firm fixation of the culture dish, start the push rod motor, and the driving plate pulls the clamping seat through the connecting rope to firmly clamp the culture dish, preventing it from shaking and displacement during the culture process, thus ensuring the stable growth of stem cells.

[0019] 2. Specific and precise temperature and oxygen control performance: the temperature sensor monitors the temperature inside the box and cooperates with the heating and cooling plates to accurately control the temperature; the oxygen concentration sensor detects in real time, controls the oxygen supply of the gas cylinder through the solenoid valve, and adjusts the oxygen concentration to provide a suitable environment for each growth stage of stem cells.

[0020] 3. Specific real-time monitoring performance: the drive motor drives the microscope camera to rotate, collecting stem cell images and nutrient concentration data, so that researchers can timely understand the cell growth status and adjust the culture strategy.

[0021] 4. Specific comprehensive acquisition performance: when the vertical plate rotates, the lifting plate drives the microscope camera to move under the action of the spiral plate, realizing the full-dimensional acquisition of stem cell images in the culture dish, avoiding monitoring blind spots and improving data accuracy. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is a schematic diagram of the three-dimensional structure of a precise culture device for MANF gene-modified umbilical cord mesenchymal stem cells proposed in the present invention;

[0023] Figure 2 This is a schematic diagram of the cross-sectional three-dimensional structure of a precise culture device for MANF gene-modified umbilical cord mesenchymal stem cells proposed in the present invention;

[0024] Figure 3 for Figure 2 A schematic diagram of a three-dimensional structure viewed from above;

[0025] Figure 4 This is a schematic diagram of the partial three-dimensional structure of a precise culture device for MANF gene-modified umbilical cord mesenchymal stem cells proposed in the present invention;

[0026] Figure 5 for Figure 4A schematic diagram of a partially cutaway three-dimensional structure;

[0027] Figure 6 for Figure 4 Schematic diagram of the local three-dimensional structure;

[0028] Figure 7 for Figure 5 Schematic diagram of the structure of part A;

[0029] Figure 8 for Figure 4 Schematic diagram of the local three-dimensional structure;

[0030] Figure 9 for Figure 8 A schematic diagram of a three-dimensional structure viewed from above;

[0031] Figure 10 for Figure 9 Schematic diagram of the structure of part B;

[0032] Figure 11 This is a schematic diagram of the three-dimensional structure of the annular fixed plate and spiral plate of the precise culture device for MANF gene-modified umbilical cord mesenchymal stem cells proposed in the present invention.

[0033] In the figure: 101, incubator; 102, cover; 103, observation window; 104, controller; 105, support rod; 106, placement table; 107, culture dish; 108, ring plate; 201, heating plate; 202, cooling plate; 203, temperature sensor; 204, oxygen concentration sensor; 205, chamber; 301, gas cylinder; 302, outlet pipe; 303, mixing box; 304, inlet pipe; 401, moving groove; 402, clamping seat; 403, fixing spring; 404, guide plate; 405, guide groove; 406, wire rod; 407, connecting rod Connecting rope; 408, driving plate; 409, push rod motor; 501, annular rotating plate; 502, annular rack; 503, mounting bracket; 504, driving motor; 505, driving gear; 506, annular slide; 507, annular slide; 601, vertical plate; 602, horizontal bar; 603, sliding seat; 604, microscope camera; 701, annular fixing plate; 702, spiral plate; 703, lifting slot; 704, lifting plate; 801, driving seat; 802, rack 1; 803, pushing seat; 804, rack 2; 805, connecting seat; 806, rotating gear. DETAILED DESCRIPTION

[0034] The technical solutions of the present invention will be described clearly and completely below with reference to specific embodiments. Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. All other embodiments derived by persons of ordinary skill in the art based on the embodiments of the present invention without inventive effort are also within the scope of protection of the present invention.

[0035] When a component is referred to as being "disposed on" another component, it can be directly on the other component or there can be an intervening component. "Disposed" indicates a way of existence, which can be a connection method such as connection, installation, fixed connection, active connection, etc. When a component is considered to be "connected" to another component, it can be directly connected to the other component or there can be an intervening component at the same time.

[0036] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this invention pertains. The terms used herein in the specification of the present invention are for the purpose of describing specific embodiments only and are not intended to limit the present invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0037] Reference Figure 1-11 A precise culture device for MANF gene-modified umbilical cord mesenchymal stem cells comprises an incubator 101, a lid 102 is provided on the top of the incubator 101, an observation window 103 is provided on the top of the lid 102, a plurality of support rods 105 are fixedly installed on the bottom inner wall of the incubator 101, a same placement platform 106 is fixedly installed on the top of the plurality of support rods 105, a culture dish 107 is placed on the top of the placement platform 106, and a clamping mechanism is provided between the placement platform 106 and the culture dish 107; a chamber 205 is provided on the incubator 101, and the chamber 205 is provided on the bottom inner wall of the incubator 101. A heating plate 201 is installed on the inner wall of the chamber 205, a cooling plate 202 is installed on the bottom inner wall of the chamber 205, a temperature sensor 203 and an oxygen concentration sensor 204 are installed on the inner wall of one side of the incubator 101, a gas cylinder 301 is installed on one side of the incubator 101, an outlet pipe 302 is installed at the bottom of the gas cylinder 301, a mixing box 303 is installed at the bottom of the outlet pipe 302, and an inlet pipe 304 connected to the incubator 101 is installed on one side of the mixing box 303. Solenoid valves are installed on both the outlet pipe 302 and the inlet pipe 304.

[0038] The clamping mechanism includes a movable groove 401, a clamping seat 402 and a fixed spring 403. The top of the placement table 106 is provided with a plurality of movable grooves 401 in a circular shape at equal intervals. A clamping seat 402 for fixing the culture dish 107 is slidably installed in the movable groove 401, and a fixed spring 403 is fixedly installed on one side of the clamping seat 402. One end of the fixed spring 403 is fixedly installed on the inner wall of the movable groove 401. An annular plate 108 is fixedly installed on the bottom of the placement table 106, and an annular rotating plate 501 rotatably connected to the placement table 106 is rotatably installed on the top of the annular plate 108. A vertical plate 601 is fixedly installed on the top of the vertical plate 601, and a cross bar 602 is fixedly installed on the top of the vertical plate 601. A sliding seat 603 is slidably sleeved on the cross bar 602, and a microscope camera 604 for image acquisition is fixedly installed on the sliding seat 603.

[0039] In this embodiment, a push rod motor 409 is fixedly mounted on the bottom of the placement table 106. A drive plate 408 is fixedly mounted on the output shaft of the push rod motor 409. A pulling mechanism is provided between the drive plate 408 and the clamping seat 402. The pulling mechanism includes a guide slot 405, a wire rod 406, and a connecting rope 407. A guide slot 405 is defined on one inner wall of the movable slot 401. Wire rods 406 are fixedly mounted on both inner walls of the guide slot 405. A connecting rope 407 is fixedly mounted on one side of the clamping seat 402. One end of the connecting rope 407 passes through the wire rod 406 and is fixedly mounted on the drive plate 408. This design utilizes the power of the push rod motor 409 to precisely control the clamping seat 402 via the pulling mechanism, enabling rapid and stable clamping and release of the culture dish 107. This ensures that the culture dish 107 is securely held during the culture process and prevents the stability of the stem cell culture environment from being affected by shaking of the culture dish 107.

[0040] In this embodiment, limit slots are defined on both inner walls of the movable groove 401. Limit seats are fixedly mounted on both sides of the clamping seat 402, and the limit seats are slidably connected to the corresponding limit slots. This limit structure effectively restricts the movement direction of the clamping seat 402 within the movable groove 401, preventing the clamping seat 402 from shifting or shaking during movement. This further improves the reliability and stability of the clamping seat 402's grip on the culture dish 107, thereby ensuring the accuracy of stem cell culture.

[0041] In this embodiment, an annular rack 502 is mounted on the outer side of the annular rotating plate 501, a mounting bracket 503 is mounted on the bottom inner wall of the incubator 101, a drive motor 504 is fixedly mounted on the top of the mounting bracket 503, a drive gear 505 is fixedly mounted on the output shaft of the drive motor 504, and the drive gear 505 meshes with the annular rack 502. An annular chute 506 is defined on the outer side of the placement table 106, and an annular slide 507 is fixedly mounted on the inner wall of the annular rotating plate 501, and the annular slide 507 is rotatably connected to the annular chute 506. This combination of transmission and guide structures enables the drive motor 504 to stably and efficiently drive the annular rotating plate 501 to rotate, thereby driving the microscope camera 604 to rotate around the culture dish 107, providing a stable motion foundation for comprehensively capturing stem cell images and monitoring nutrient concentrations.

[0042] In this embodiment, an annular fixing plate 701 is fixedly mounted on the top of the placement platform 106, and a spiral plate 702 is fixedly mounted on the outer side of the annular fixing plate 701. A lifting groove 703 is defined on one side of the vertical plate 601, and a lifting plate 704 is slidably mounted within the lifting groove 703. The lifting plate 704 is slidably mounted between the spiral plates 702. This structure utilizes the characteristics of the spiral plates 702, allowing the vertical plate 601 to rotate and drive the lifting plate 704 to rise in a spiral direction. This allows the micro camera 604 to simultaneously rotate horizontally and move vertically, greatly expanding the acquisition range of the micro camera 604 and enabling more comprehensive image capture of the stem cells in the culture dish 107.

[0043] In this embodiment, two connecting blocks 805 are fixedly mounted on one side of the vertical plate 601. A rotating gear 806 is rotatably mounted between the two connecting blocks 805. A transmission mechanism is provided between the lifting plate 704 and the microscopic camera 604. The transmission mechanism includes a drive block 801, a rack 1 802, a pusher block 803, and a rack 2 804. The drive block 801 is fixedly mounted on the top of the lifting plate 704, the rack 1 802 is fixedly mounted on one side of the drive block 801, the pusher block 803 is fixedly mounted on one side of the microscopic camera 604, and the rack 2 804 is fixedly mounted on the top of the pusher block 803. Both racks 1 802 and 804 mesh with the rotating gear. This transmission mechanism, through the coordination of the gears and racks, converts the vertical motion of the lifting plate 704 into horizontal motion of the microscopic camera 604, enabling precise adjustment of the microscopic camera 604 to different positions, ensuring clear acquisition of stem cell images from all areas within the culture dish 107.

[0044] In this embodiment, a controller 104 is mounted on top of the chamber lid 102, and the temperature sensor 203, oxygen concentration sensor 204, push rod motor 409, drive motor 504, and solenoid valve are all electrically connected to the controller 104. Through the unified control of each component by the controller 104, it is possible to receive sensor monitoring data in real time and accurately, and automatically control the operation of related equipment according to a preset program, achieving precise adjustment of culture environment parameters and intelligent control of operations such as the fixation of the culture dish 107 and image acquisition, thereby improving the automation level and culture efficiency of the culture device.

[0045] In the present invention, when the MANF gene modified umbilical cord mesenchymal stem cells are precisely cultured, the culture dish 107 is placed on the top of the placement table 106, and the push rod motor 409 is started. The push rod motor 409 can drive the driving plate 408 to move downward through the output shaft. The driving plate 408 can pull the clamping seat 402 through the connecting rope 407. The clamping seat 402 can move toward the direction of the culture dish 107 and clamp the culture dish 107. The temperature sensor 203 can be set to the incubator. The temperature inside the incubator 101 is monitored. When the temperature is different from the set value, the heating plate 201 can heat the incubator 101 to increase the temperature of the incubator 101, and the cooling plate 202 can cool the incubator 101, thereby achieving the purpose of temperature control of the incubator 101. When the oxygen concentration is different, the solenoid valve can be opened to allow the oxygen in the gas cylinder 301 to enter the mixing box 303 for mixing and then enter the incubator 101 through the connecting pipe, thereby achieving the purpose of adjusting the oxygen concentration.

[0046] By starting the driving motor 504, the driving motor 504 can drive the driving gear 505 to rotate, and the driving gear 505 can drive the annular rotating plate 501 to rotate through the annular rack 502. The annular rotating plate 501 can drive the horizontal bar 602 and the microscopic camera 604 to rotate through the vertical plate 601. The image and nutrient concentration of the stem cells can be collected through the microscopic camera 604, and the growth status of the cells and the changes in the culture environment can be understood in a timely manner. When the vertical plate 601 rotates, the lifting plate 704 can follow The vertical plate 601 rotates, and under the action of the spiral plate 702, the lifting plate 704 can move upward, and the lifting plate 704 can lead the driving seat 801 and rack 1 802 to move upward, and the rack 1 802 can lead the rotating gear 806 to rotate counterclockwise, and the counterclockwise rotating rotating gear 806 can lead the rack 2 804 and the pushing seat 803 to move, and the pushing seat 803 can lead the microscope camera 604 to move, so as to achieve comprehensive and sufficient collection of images of the stem cells in the culture dish 107.

[0047] The above describes in detail the precise culture device for MANF gene-modified umbilical cord mesenchymal stem cells provided by the present invention. Specific examples are used herein to illustrate the principles and implementation methods of the present invention. The description of the above examples is intended only to facilitate understanding of the methods and core concepts of the present invention. It should be noted that those skilled in the art will be able to make various improvements and modifications to the present invention without departing from the principles of the present invention, and such improvements and modifications are also within the scope of protection of the claims of the present invention.

Claims

1. A precise culture device for MANF gene-modified umbilical cord mesenchymal stem cells, characterized in that: The incubator (101) comprises an incubator (101), wherein a cover (102) is provided on the top of the incubator (101), an observation window (103) is provided on the top of the cover (102), a plurality of support rods (105) are fixedly mounted on the inner wall of the bottom of the incubator (101), a same placement platform (106) is fixedly mounted on the tops of the plurality of support rods (105), a culture dish (107) is placed on the top of the placement platform (106), and a clamping mechanism is provided between the placement platform (106) and the culture dish (107); The incubator (101) is provided with a chamber (205), a heating plate (201) is installed on the inner wall of the chamber (205), a cooling plate (202) is installed on the inner wall of the bottom of the chamber (205), a temperature sensor (203) and an oxygen concentration sensor (204) are installed on the inner wall of one side of the incubator (101), a gas cylinder (301) is installed on one side of the incubator (101), an air outlet pipe (302) is installed at the bottom of the gas cylinder (301), a mixing box (303) is installed at the bottom of the air outlet pipe (302), an air inlet pipe (304) connected to the incubator (101) is installed on one side of the mixing box (303), and both the air outlet pipe (302) and the air inlet pipe (304) are provided with solenoid valves; The clamping mechanism comprises a movable groove (401), a clamping seat (402) and a fixed spring (403); the top of the placement platform (106) is provided with a plurality of movable grooves (401) at equal intervals in a circular shape; a clamping seat (402) for fixing the culture dish (107) is slidably installed in the movable groove (401); a fixed spring (403) is fixedly installed on one side of the clamping seat (402); and one end of the fixed spring (403) is fixedly installed on the inner wall of the movable groove (401); An annular plate (108) is fixedly installed at the bottom of the placement platform (106), an annular rotating plate (501) rotatably connected to the placement platform (106) is rotatably installed at the top of the annular rotating plate (501), a vertical plate (601) is fixedly installed at the top of the vertical plate (601), a cross bar (602) is fixedly installed at the top of the vertical plate (601), a sliding seat (603) is slidably sleeved on the cross bar (602), and a microscopic camera (604) for image acquisition is fixedly installed on the sliding seat (603).

2. A precise culture device for MANF gene-modified umbilical cord mesenchymal stem cells according to claim 1, characterized in that: A push rod motor (409) is fixedly mounted on the bottom of the placement platform (106), a driving plate (408) is fixedly mounted on the output shaft of the push rod motor (409), a pulling mechanism is provided between the driving plate (408) and the clamping seat (402), a guide plate (404) is fixedly mounted on the bottom of the placement platform (106), and the driving plate (408) is slidably sleeved on (404).

3. A precise culture device for MANF gene-modified umbilical cord mesenchymal stem cells according to claim 2, characterized in that: The pulling mechanism comprises a guide groove (405), a conductor rod (406) and a connecting rope (407); a guide groove (405) is provided on an inner wall of one side of the movable groove (401); conductor rods (406) are fixedly mounted on inner walls on both sides of the guide groove (405); a connecting rope (407) is fixedly mounted on one side of the clamping seat (402); one end of the connecting rope (407) is passed around the conductor rod (406) and fixedly mounted on the driving plate (408).

4. A precise culture device for MANF gene-modified umbilical cord mesenchymal stem cells according to claim 1, characterized in that: Limiting grooves are provided on both inner walls of the movable groove (401), and limiting seats are fixedly installed on both sides of the clamping seat (402), and the limiting seats are slidably connected to the corresponding limiting grooves.

5. A precise culture device for MANF gene-modified umbilical cord mesenchymal stem cells according to claim 1, characterized in that: An annular rack (502) is installed on the outer side of the annular rotating plate (501), a mounting frame (503) is installed on the bottom inner wall of the incubator (101), a driving motor (504) is fixedly installed on the top of the mounting frame (503), a driving gear (505) is fixedly installed on the output shaft of the driving motor (504), and the driving gear (505) is meshed with the annular rack (502).

6. A precise culture device for MANF gene-modified umbilical cord mesenchymal stem cells according to claim 1, characterized in that: An annular chute (506) is provided on the outer side of the placement platform (106), and an annular slide seat (507) is fixedly installed on the inner wall of the annular rotating plate (501), and the annular slide seat (507) is rotatably connected to the annular chute (506).

7. The precise culture device for MANF gene-modified umbilical cord mesenchymal stem cells according to claim 1, characterized in that: An annular fixing plate (701) is fixedly installed on the top of the placement platform (106), a spiral plate (702) is fixedly installed on the outer side of the annular fixing plate (701), a lifting groove (703) is opened on one side of the vertical plate (601), a lifting plate (704) is slidably installed in the lifting groove (703), and the lifting plate (704) is slidably installed between the spiral plates (702).

8. The precise culture device for MANF gene-modified umbilical cord mesenchymal stem cells according to claim 1, characterized in that: Two connecting seats (805) are fixedly installed on one side of the vertical plate (601), a rotating gear (806) is rotatably installed between the two connecting seats (805), and a transmission mechanism is provided between the lifting plate (704) and the microscopic camera (604).

9. A precise culture device for MANF gene-modified umbilical cord mesenchymal stem cells according to claim 8, characterized in that: The transmission mechanism includes a driving seat (801), a rack 1 (802), a pushing seat (803) and a rack 2 (804), wherein the driving seat (801) is fixedly mounted on the top of the lifting plate (704), the rack 1 (802) is fixedly mounted on one side of the driving seat (801), the pushing seat (803) is fixedly mounted on one side of the micro camera (604), and the rack 2 (804) is fixedly mounted on the top of the pushing seat (803), and both the rack 1 (802) and the rack 2 (804) are engaged with the rotating gear.

10. The precise culture device for MANF gene-modified umbilical cord mesenchymal stem cells according to claim 1, characterized in that: A controller (104) is installed on the top of the box cover (102), and the temperature sensor (203), the oxygen concentration sensor (204), the push rod motor (409), the drive motor (504) and the solenoid valve are all electrically connected to the controller (104).

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