Sampling and spotting method

Abstract

The invention discloses a sampling and spotting method, which uses an inner needle (1) and an outer needle (2) which are sleeved with each other for sampling, and comprises the following steps: S1, the outer needle (2) descends to puncture, and then the inner needle (1) descends to suck a sample; s2, the inner needle (1) ascends and then the outer needle (2) ascends, or the inner needle (1) and the outer needle (2) ascend together; s3, descending the inner needle (1), and spotting the carrier; and S4, cleaning the inner needle (1). According to the sampling and spotting method, the sequence and operation actions of all the steps are limited, so that it is guaranteed that sampling is smooth, cleaning is sufficient, cross contamination is avoided, the working efficiency is high, and it can be guaranteed that the inner needle and the outer needle are not prone to damage and more durable.

Description

Technical Field

[0001] The present application relates to the technical field of detection equipment, and in particular to a sampling method. Background Art

[0002] Microscopic examination of blood smears is a basic method of blood cytology examination, especially for the diagnosis of various blood diseases. It is widely used in clinical examinations.

[0003] The blood sample sent for testing is sealed and stored in a blood collection tube. The analytical instrument needs to use a sampling needle to pierce the sealing rubber stopper of the blood collection tube, insert it into the tube to absorb the blood sample, and then smear it on a glass slide to form a blood smear, which is then examined under a microscope.

[0004] To prevent damage to the sampling needle, a sheathed inner and outer needle configuration is now commonly used, with the outer needle performing puncture and the inner needle taking the sample. After each sampling session, the sampling needle must be cleaned to prevent cross-contamination. The efficiency of the sampling needle, particularly its sampling efficiency, and the thoroughness of its cleaning significantly impact the analyzer's operating efficiency and the accuracy of its results. Summary of the Invention

[0005] To solve the above technical problems, the first object of the present invention is to provide a sampling method. The sampling method provided in this application defines the sequence of each step and the operation actions to ensure smooth sampling, sufficient cleaning, no cross contamination, high work efficiency, and can ensure that the inner and outer needles are not easily damaged and are more durable.

[0006] The technical solutions provided by the present invention are as follows:

[0007] A method for sampling and spotting, using an inner needle and an outer needle arranged one above the other for sampling, comprises the following steps:

[0008] S1: The outer needle descends to puncture, and then the inner needle descends to absorb the sample;

[0009] S2, the inner needle rises and then the outer needle rises, or the inner needle and the outer needle rise together;

[0010] S3, the inner needle descends and spots the sample on the carrier;

[0011] S4. Clean the inner needle.

[0012] Preferably, the method further includes step S0, the inner needle and the outer needle start from the origin and move to the sampling position, and then execute step S1;

[0013] After completing step S2, the inner needle and the outer needle are moved from the sampling position to the sample spotting position, and then step S3 is performed;

[0014] After completing step S3, the inner needle and the outer needle move to the origin and step S4 is executed.

[0015] Preferably, a cleaning pool is provided at the origin.

[0016] Preferably, an inner needle swab is sheathed outside the inner needle, and the top of the inner needle is also connected to a top cleaning tube;

[0017] Step S4 includes:

[0018] S4-1, the cleaning liquid enters the inner needle swab, and the inner needle rises and falls relative to the inner needle swab to clean the outer wall of the inner needle;

[0019] S4-2, the cleaning liquid enters the top cleaning tube to clean the inside of the inner needle;

[0020] Steps S4-1 and S4-2 can be performed simultaneously, or optionally performed one after another.

[0021] Preferably, after completing step S1 and before executing step S3, the method further includes the step of using an inner needle swab to absorb a sample from the outer wall of the inner needle; or

[0022] An outer needle swab is sheathed outside the outer needle. After completing step S1 and before executing step S3, the step of using the outer needle swab to absorb a sample from the outer wall of the inner needle is also included.

[0023] Preferably, step S4 further includes:

[0024] S4-3, the cleaning liquid enters the inner needle swab, cleans the inner wall of the outer needle, and then falls into the cleaning pool;

[0025] S4-4, the cleaning liquid enters the outer needle swab or is sucked outward from the outer needle swab, and the outer needle rises and falls relative to the outer needle swab to clean the outer wall of the outer needle;

[0026] Steps S4-3 and S4-4 can be performed simultaneously, or optionally performed one after the other, or performed one at a time.

[0027] Preferably, the outer needle is fixed to the inner needle swab.

[0028] Preferably, step S3 specifically includes: after the inner needle descends, it moves relative to the carrier to perform sample spotting.

[0029] Preferably, the amount of sample drawn by the inner needle in step S1 is greater than the amount required for two spotting operations;

[0030] After executing step S3, wait for the detection result of the carrier; if the carrier is qualified, execute step S4; if the carrier is unqualified, repeat step S3 and spot a new carrier.

[0031] Preferably, the outer needle is provided with a ventilation groove penetrating the needle wall, and the ventilation groove extends along the axis direction of the outer needle; or,

[0032] The outer needle is connected to the outside atmosphere.

[0033] To address the problems of the existing technology, the present application provides a sampling and spotting method using an inner and outer needle nested within each other for sampling, comprising the following steps: S1: The outer needle descends to puncture, then the inner needle descends to absorb the sample; S2: The inner needle ascends, then the outer needle ascends, or both the inner and outer needles ascend together; S3: The inner needle descends to spot the sample on the carrier; S4: Cleaning the inner needle. The sampling and spotting method provided in this application defines the sequence and operation of each step to ensure smooth and thorough sampling, prevent cross-contamination, and achieve high efficiency. It also ensures that the inner and outer needles are not easily damaged, making them more durable. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments recorded in this application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0035] Figure 1 Schematic diagram of the structure of a device that can use the above-mentioned sampling method in an embodiment of the present invention;

[0036] Figure 2 for Figure 1 A partial enlarged view of

[0037] Figure 3 This is a schematic structural diagram of an inner needle swab as an implementation structure in an embodiment of the present invention;

[0038] Figure 4 A schematic cross-sectional view of an inner needle swab as an implementation structure in an embodiment of the present invention;

[0039] Figure 5 A schematic cross-sectional view of an inner needle swab as an implementation structure in an embodiment of the present invention (the inner needle is not shown);

[0040] Figure 6 This is a cross-sectional schematic diagram from another angle of an inner needle swab as an implementation structure in an embodiment of the present invention (the inner needle is not shown);

[0041] Figure markings: 1-inner needle; 2-outer needle; 21-ventilation groove; 3-cleaning pool; 4-inner needle swab; 41-inner needle body; 42-inner needle cleaning cavity; 43-inner needle cleaning inlet; 44-inner needle cleaning outlet; 45-inner needle channel; 5-outer needle swab. DETAILED DESCRIPTION

[0042] In order to help those skilled in the art better understand the technical solutions in this application, the technical solutions in the embodiments of this application will be clearly and completely described below. Obviously, the embodiments described are only part of the embodiments of this application, not all of the embodiments. Based on the embodiments in this application, all other embodiments obtained by those skilled in the art without making any creative efforts shall fall within the scope of protection of this application.

[0043] It should be noted that when an element is referred to as being “fixed on” or “set on” another element, it can be directly on the other element or indirectly set on the other element; when an element is referred to as being “connected to” another element, it can be directly connected to the other element or indirectly connected to the other element.

[0044] It should be understood that the terms "length", "width", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the accompanying drawings, and are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on this application.

[0045] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be understood to indicate or imply relative importance or implicitly specify the number of technical features indicated. Thus, a feature specified as "first" or "second" may explicitly or implicitly include one or more of such features. Throughout the description of this application, "plurality" or "several" means two or more, unless otherwise specifically defined.

[0046] It should be noted that the structures, proportions, sizes, etc. illustrated in the drawings of this specification are only used to match the contents disclosed in the specification for people familiar with this technology to understand and read, and are not used to limit the conditions under which this application can be implemented. Therefore, they have no substantive technical significance. Any structural modification, change in proportional relationship or adjustment of size should still fall within the scope of the technical content disclosed in this application without affecting the efficacy and purpose that can be achieved by this application.

[0047] As shown in the figure, an embodiment of the present invention provides a sampling method, which uses an inner needle 1 and an outer needle 2 arranged in a nested manner to perform sampling, including the following steps:

[0048] S1, outer needle 2 descends to puncture, then inner needle 1 descends to absorb the sample;

[0049] S2, inner needle 1 rises and then outer needle 2 rises, or inner needle 1 and outer needle 2 rise together;

[0050] S3, inner needle 1 descends to spot the carrier;

[0051] S4. Clean the inner needle 1.

[0052] To address the problems of the prior art, the present application provides a sampling and spotting method using an inner needle 1 and an outer needle 2 nested within each other for sampling, comprising the following steps: S1: Outer needle 2 descends to puncture, then inner needle 1 descends to absorb the sample; S2: Inner needle 1 ascends, then outer needle 2 ascends, or both inner needle 1 and outer needle 2 ascend together; S3: Inner needle 1 descends to spot the sample on the carrier; S4: Cleaning inner needle 1. The sampling and spotting method provided in this application defines the sequence and operational actions of each step to ensure smooth sampling, adequate cleaning, and no cross-contamination, while also ensuring high efficiency. It also ensures that inner needle 1 and outer needle 2 are less susceptible to damage and more durable.

[0053] Specifically, in step S1, outer needle 2 descends to puncture the sealing stopper of the blood collection tube. Then, inner needle 1, located within outer needle 2, descends again, passing through the punctured sealing stopper and extending into the tube to aspirate the sample. Because outer needle 2 has a larger diameter than inner needle 1, it is less susceptible to damage. Furthermore, the outer needle 2 can be thicker, further enhancing its durability.

[0054] After the inner needle 1 completes step S1, a sufficient amount of sample has been drawn into the inner needle 1, and step S2 is then executed. The inner needle 1 can be initially raised while the outer needle 2 remains stationary, maintaining its position within the stopper to prevent the stopper from obstructing the inner needle 1. After the inner needle 1 is raised, the outer needle 2 is then raised, allowing both needles 1 and 2 to be released from the blood collection tube. Alternatively, the inner needle 1 and outer needle 2 can be raised together, allowing both needles 1 and 2 to be released from the tube, preventing collision with the tube that could damage the needles or cause sample spillage. In step S2, the height to which the inner and outer needles 1 and 2 are raised can be determined as needed; at a minimum, both needles 1 and 2 should be elevated above the top of the tube, allowing them to be released from the tube.

[0055] After step S2 is completed, step S3 is executed, in which the inner needle 1 descends and spots the sucked sample onto the carrier, and the sample carrier is prepared and sent for inspection.

[0056] After step S3 is completed, step S4 is executed to clean the inner needle 1 to prevent the residue of the previous sample sucked by the inner needle 1 from mixing into the next sample and affecting the detection accuracy of the next sample.

[0057] In the present application, the movement of the inner needle 1 and the outer needle 2 can be achieved by respectively providing a driving power source and a transmission structure (such as a gear rack, a timing belt, etc.). The inner needle 1 can absorb the sample by using a negative pressure generating device to provide negative pressure. At the same time, after the inner needle 1 absorbs the sample and moves, the negative pressure can also be used to maintain the sample in the inner needle tube of the inner needle 1 and prevent it from falling out.

[0058] Preferably, the method further includes step S0, the inner needle 1 and the outer needle 2 start from the origin and move to the sampling position, and then execute step S1;

[0059] After completing step S2, the inner needle 1 and the outer needle 2 are moved from the sampling position to the sample application position, and then step S3 is performed;

[0060] After completing step S3, the inner needle 1 and the outer needle 2 move to the origin and step S4 is executed.

[0061] The inner needle 1 and outer needle 2 can be raised and lowered at the same horizontal position to sequentially perform puncture, sample aspiration, sample spotting, and cleaning operations. This can be accomplished by using other structures in conjunction with moving the blood collection tube, carrier, etc. However, it is more preferred that each step be performed at different horizontal positions, with the inner needle 1 and outer needle 2 being translated to the origin, sampling position, or sample spotting position, while the blood collection tube and carrier, etc., can be continuously transported and delivered to the corresponding positions for sampling or sample spotting, thereby improving work efficiency.

[0062] Specifically, before executing step S1, the process also includes step S0, where the inner needle 1 and outer needle 2 start from the origin and move to the sampling position. After step S0 is completed, the inner needle 1 and outer needle 2 are located at the sampling position, and the blood collection tube to be sampled is also delivered to the sampling position by other structures (the blood collection tube can arrive at the sampling position before or after the needle arrives, and the order is not limited). It is located below the inner needle 1 and outer needle 2, and then steps S1 and S2 are executed. After step S2 is completed, the inner needle 1 and outer needle 2 move from the sampling position to the sample application position, and the carrier to be sampled is also moved to the sample application position by the corresponding structure (again, the order is not limited), and is located below the inner needle 1 and outer needle 2. Then step S3 is executed.

[0063] After completing step S3, the inner needle 1 and the outer needle 2 are moved to the origin, and step S4 is performed so that the cleaning work does not interfere with the movement of the carrier. Moreover, when using a cleaning liquid for cleaning, cleaning at the origin can also prevent the cleaning liquid from contaminating the carrier.

[0064] The origin can be located between the sampling and spotting positions, on the side of the sampling position away from the spotting position, or on the side of the spotting position away from the sampling position. The only difference is the movement path, but all three can achieve the sampling, spotting, and cleaning operations. It is preferred that the origin, sampling position, and spotting position be located in a straight line and move in a straight line rather than within a plane.

[0065] The translation of the inner needle 1 and the outer needle 2 can be achieved by a horizontally movable power source and a transmission mechanism. By arranging the device for driving the inner needle 1 and the outer needle 2 to move upward and downward on the mounting plate, and then using the horizontally movable drive mechanism to drive the mounting plate to move horizontally, interference can be avoided.

[0066] Preferably, a cleaning pool 3 is provided at the origin.

[0067] Preferably, a cleaning pool 3 is provided at the origin to receive the mixed solution of the dropped cleaning liquid and the residual sample, etc., so as to facilitate the cleaning of the sample suction component and prevent the sample liquid from dripping onto the instrument table.

[0068] Preferably, an inner needle swab 4 is sheathed outside the inner needle 1, and the top of the inner needle 1 is also connected to a top cleaning tube;

[0069] Step S4 includes:

[0070] S4-1, the cleaning liquid enters the inner needle swab 4, and the inner needle 1 rises and falls relative to the inner needle swab 4 to clean the outer wall of the inner needle 1;

[0071] S4-2, the cleaning liquid enters the top cleaning tube to clean the inside of the inner needle 1;

[0072] Steps S4-1 and S4-2 can be performed simultaneously, or optionally performed one after another.

[0073] To facilitate cleaning, the inner needle 1 is preferably equipped with a corresponding inner needle swab 4, and a top cleaning tube is also connected to the top of the inner needle 1. When using this structure for cleaning, step S4 includes cleaning the inner and outer walls of the inner needle 1. Specifically, the outer wall of the inner needle 1 is cleaned by injecting liquid through the inner needle swab 4, while the inner wall of the inner needle 1 is cleaned by injecting liquid through the top cleaning tube. These two steps can be performed simultaneously or sequentially.

[0074] Because the inner needle 1 contacts the sample in each blood collection tube for sampling, it needs to be thoroughly cleaned to avoid cross contamination. Therefore, steps S4-1 and S4-2 are both aimed at cleaning the inner needle 1, cleaning the inner wall (including the contents) and outer wall of the inner needle 1. Step S4-2 may also clean the inner wall of the outer needle 2 and the space between the inner needle 1 and the outer needle 2, but this is not the main purpose of step S4-2. Depending on the suction force of the inner needle swab 4, the cleaning liquid sprayed from the liquid inlet of the inner needle swab 4 can be completely absorbed by the liquid outlet provided by the swab. At this time, the cleaning liquid will not flow down along the inner wall of the outer needle 2 and will not clean the inner wall of the outer needle 2. However, during cleaning, the inner needle 1 is raised and lowered relative to the inner needle swab 4, which can ensure that the outer wall of the inner needle 1 is fully cleaned. When the cleaning liquid is not completely absorbed by the inner needle swab 4, the cleaning liquid flowing down between the inner and outer needles can play a certain role in cleaning the inner wall of the outer needle 2.

[0075] The cleaning liquid can be delivered to the corresponding location through the pipeline by the power of the pump.

[0076] Preferably, after completing step S1 and before executing step S3, the method further includes the step of using the inner needle swab 4 to absorb a sample from the outer wall of the inner needle 1; or

[0077] An outer needle swab 5 is sheathed on the outer surface of the outer needle 2 . After completing step S1 and before executing step S3 , the step of using the outer needle swab 5 to absorb a sample from the outer wall of the inner needle 1 is also included.

[0078] After the inner needle 1 absorbs the sample, it is very likely that some sample will remain on the outer wall, and it is difficult to control whether this part of the sample will drip. After completing step S1 and before performing step S3, the sample remaining on the outer wall may contaminate the instrument once it drips, which will have an adverse effect on the detection. Therefore, it is preferred that after completing step S1 and before performing step S3, the step of using the inner needle swab 4 to absorb the sample from the outer wall of the inner needle 1 is also included; or, after completing step S1 and before performing step S3, the outer needle swab 5 is set outside the outer needle 2, and after completing step S1 and before performing step S3, the step of using the outer needle swab 5 to absorb the sample from the outer wall of the inner needle 1 is also included. Whether to use the inner needle swab 4 or the outer needle swab 5 to absorb the residual sample on the outer wall of the inner needle 1 can be selected according to actual needs. When using the inner needle swab 4 for absorption, only the relative position of the inner needle swab 4 and the inner needle 1 needs to be adjusted, while when using the outer needle swab 5 for absorption, the outer needle swab 5 needs to be placed at the bottom end of the outer needle 2 or lower, and then the relative position of the outer needle swab 5 and the inner needle 1 needs to be adjusted for absorption.

[0079] Preferably, step S4 further includes:

[0080] S4-3, the cleaning liquid enters the inner needle swab 4, cleans the inner wall of the outer needle 2, and then falls into the cleaning pool 3;

[0081] S4-4, the cleaning liquid enters the outer needle swab 5 or is sucked outward by the outer needle swab 5, and the outer needle 2 rises and falls relative to the outer needle swab 5 to clean the outer wall of the outer needle 2;

[0082] Steps S4-3 and S4-4 can be performed simultaneously, or optionally performed one after the other, or performed one at a time.

[0083] Preferably, the outer needle 2 is also cleaned in step S4. Since the outer needle 2 is used to puncture the stopper, contact with the sample can be avoided by controlling its puncture depth. The main thing to avoid is that if the outer needle 2 accidentally touches the sample during the previous puncture process, the residual sample will contaminate the next sample. Therefore, cleaning the outer needle 2 is more effective.

[0084] Cleaning of the outer needle 2 is divided into cleaning of the inner and outer walls of the outer needle 2. Cleaning of the inner wall of the outer needle 2 can be performed simultaneously with cleaning of the outer wall of the inner needle 1, or separately. When performed separately, step S4-3 is performed separately, and the cleaning liquid falls into the cleaning pool 3 and is collected. Cleaning of the outer wall of the outer needle 2 can be performed by suction to remove residual sample, which also meets the requirements. Alternatively, cleaning liquid can be delivered to the outer needle swab 5, and the outer needle 2 can be raised and lowered relative to the outer needle swab 5 to clean the outer wall of the outer needle 2 for a more thorough cleaning.

[0085] As a specific structure that can be adopted to implement the method of the present application, the inner needle swab 4 may include an inner needle body 41, and the inner needle body 41 is provided with an inner needle cleaning cavity 42, an inner needle cleaning inlet 43, an inner needle cleaning outlet 44, and an inner needle channel 45. The inner needle cleaning inlet 43 and the inner needle cleaning outlet 44 are respectively connected to the inner needle cleaning cavity 42, and the inner needle channel 45 vertically penetrates the inner needle body 41 and is connected to the inner needle cleaning cavity 42; in step S4-1, the cleaning liquid enters the inner needle cleaning cavity 42 from the inner needle cleaning inlet 43 to clean the outer wall of the inner needle 1, and then is discharged from the inner needle cleaning outlet 44 or falls into the cleaning pool 3 from the inner needle channel 45.

[0086] The outer needle swab 5 can adopt a swab structure known in the art, including a suction outlet and an outer needle channel, and whether to set an inlet for the cleaning liquid is determined according to whether suction or input of the cleaning liquid is used in step S4-4.

[0087] Preferably, the outer needle 2 is fixed to the inner needle swab 4 .

[0088] To simplify the structure, the outer needle 2 can be fixed to the inner needle swab 4. While maintaining the structure of the outer needle 2 sheathed within the inner needle 1, the outer needle 2 can be fixed to the bottom of the inner needle swab 4 and aligned with the inner needle passage 45. Alternatively, the outer needle 2 can be directly inserted into the inner needle passage 45 for fixation, as long as the outer needle 2 does not block the inner needle cleaning inlet 43 and the inner needle cleaning outlet 44.

[0089] Preferably, step S3 specifically includes: after the inner needle 1 descends, it moves relative to the carrier to perform sample spotting.

[0090] The preferred step S3 is as follows: after the inner needle 1 descends, it moves relative to the carrier to spot the sample, thereby forming a blood line on the carrier surface. This facilitates subsequent flattening of the sample and dispersion of blood cells, resulting in more accurate observation and detection results. The movement can be performed once or repeatedly, depending on the detection needs.

[0091] The lateral movement of the inner needle 1 is achieved by a power source and a transmission mechanism that drives the inner needle 1 and the outer needle 2 to move lateral between the origin, the sampling position, and the sample point position.

[0092] Preferably, the amount of sample drawn by the inner needle 1 in step S1 is greater than the amount required for two spotting operations;

[0093] After executing step S3, wait for the detection result of the carrier; if the carrier is qualified, execute step S4; if the carrier is unqualified, repeat step S3 and spot a new carrier.

[0094] After the sample is spotted on the carrier, it must be flattened (stripped) before it can be used for testing. Errors in spotting or rolling can lead to uneven sample distribution on the carrier, making accurate testing impossible. Therefore, testing after rolling can save time in reworking if unqualified samples are discovered.

[0095] More preferably, the amount of sample drawn by inner needle 1 in step S1 exceeds the amount required for two spotting operations; and after step S3, there is still enough sample in inner needle 1 for at least one more spotting operation. In this case, step S4 is not performed, and the test results of the carrier after the push-through are awaited. If the carrier passes the test, inner needle 1 and outer needle 2 proceed to step S4, and the remaining sample is fully discharged during the cleaning process. If the carrier fails the test, step S3 is repeated and spotting is performed on a new carrier.

[0096] Depending on the required redundancy, the amount of sample drawn by the inner needle 1 in step S1 can be set to 2-4 times the amount required for one spotting, and the sample can be spotted 2-4 times. If a qualified carrier cannot be obtained after exceeding this number, an alarm program can be introduced to require manual intervention and debugging (a repeat counting program can be set at the detection position), or a sample can be drawn from the blood collection tube again for spotting after exceeding this number.

[0097] Preferably, the outer needle 2 is provided with a ventilation groove 21 penetrating the needle wall, and the ventilation groove 21 extends along the axis direction of the outer needle 2; or,

[0098] The outer needle 2 is connected to the outside atmosphere.

[0099] When drawing the sample, there is a relatively narrow space between the inner needle 1, the outer needle 2 and the blood collection tube. The suction force may cause a large pressure difference between the inside and outside, resulting in fluctuations in the amount of sample drawn by the inner needle 1. Ventilation can effectively avoid this problem. Ventilation can be achieved by providing a ventilation groove 21 that penetrates the needle wall of the outer needle 2, and the ventilation groove 21 extends along the axial direction of the outer needle 2, connecting the inside of the blood collection tube and the outside of the rubber plug. It can also be achieved by connecting the outer needle 2 to the outside atmosphere. The position where the outer needle 2 is connected to the outside atmosphere can be at the top or side wall of the outer needle 2. More preferably, the outer needle 2 is connected to the outside atmosphere through a pipeline, and a valve is provided in the pipeline. The valve is opened when needed to connect to the atmosphere, and the valve is closed when not needed.

[0100] The above description of the disclosed embodiments is intended to enable one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be readily apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not limited to the embodiments shown herein but is intended to conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A method for sampling using an inner needle (1) and an outer needle (2) arranged one above the other for sampling, characterized in that: The following steps are involved: S1, the outer needle (2) descends to puncture, and then the inner needle (1) descends to absorb the sample; S2, the inner needle (1) rises and then the outer needle (2) rises, or the inner needle (1) and the outer needle (2) rise together; S3, the inner needle (1) descends and spots the carrier; S4. Clean the inner needle (1).

2. The sampling method according to claim 1, characterized in that: The method further includes step S0, the inner needle (1) and the outer needle (2) start from the origin and move to the sampling position, and then execute step S1; After completing step S2, the inner needle (1) and the outer needle (2) are moved from the sampling position to the sample spotting position, and then step S3 is performed; After completing step S3, the inner needle (1) and the outer needle (2) move to the origin and step S4 is executed.

3. The sampling method according to claim 2, characterized in that: A cleaning pool (3) is provided at the origin.

4. The sampling method according to claim 3, characterized in that: An inner needle swab (4) is sheathed outside the inner needle (1), and a top cleaning tube is also connected to the top of the inner needle (1); Step S4 includes: S4-1, the cleaning liquid enters the inner needle swab (4), and the inner needle (1) rises and falls relative to the inner needle swab (4) to clean the outer wall of the inner needle (1); S4-2, the cleaning liquid enters the top cleaning tube to clean the inside of the inner needle (1); Steps S4-1 and S4-2 can be performed simultaneously, or optionally performed one after another.

5. The sampling method according to claim 4, characterized in that: After completing step S1 and before executing step S3, the method further includes the step of using an inner needle swab (4) to absorb a sample from the outer wall of the inner needle (1); or, An outer needle swab (5) is sheathed outside the outer needle (2). After completing step S1 and before executing step S3, the step of using the outer needle swab (5) to absorb a sample from the outer wall of the inner needle (1) is also included.

6. The sampling method according to claim 5, characterized in that: Step S4 further includes: S4-3, the cleaning liquid enters the inner needle swab (4), cleans the inner wall of the outer needle (2), and then falls into the cleaning pool (3); S4-4, the cleaning liquid enters the outer needle swab (5) or is sucked outward by the outer needle swab (5), and the outer needle (2) rises and falls relative to the outer needle swab (5) to clean the outer wall of the outer needle (2); Steps S4-3 and S4-4 can be performed simultaneously, or optionally performed one after the other, or performed one at a time.

7. The sampling method according to claim 4, characterized in that: The outer needle (2) is fixed to the inner needle swab (4).

8. The sampling method according to claim 1, characterized in that: Step S3 specifically includes: after the inner needle (1) descends, it moves relative to the carrier to perform sample spotting.

9. The sampling method according to claim 1, characterized in that: In step S1, the amount of sample drawn by the inner needle (1) is greater than the amount required for two spottings; After executing step S3, wait for the detection result of the carrier; if the carrier is qualified, execute step S4; if the carrier is unqualified, repeat step S3 and spot a new carrier.

10. The sampling method according to claim 1, characterized in that: The outer needle (2) is provided with a ventilation groove (21) penetrating the needle wall, and the ventilation groove (21) extends along the axis direction of the outer needle (2); or, The outer needle (2) is connected to the outside atmosphere.

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