Efficient breeding method of cremastra appendiculata

Abstract

The invention provides an efficient breeding method of cremastra appendiculata, and relates to the technical field of cremastra appendiculata breeding. The efficient breeding method comprises the steps of soil preparation and land selection, strain laying, wood laying, gap filling, seed dressing, sowing, shading and management. According to the method, the specific method is adjusted, humus soil containing a compound microbial agent is adopted to fill gaps among wood, then sowing is conducted, the germination rate of seeds is increased, sawdust, perlite, coco coir, decomposed organic fertilizer, bamboo charcoal powder and pyroligneous liquor are used for seed dressing before sowing, the air permeability of a matrix is improved, seeds are prevented from being rotten due to accumulated water, and breeding of pathogenic bacteria is inhibited; the water-retaining property of the substrate is increased; and symbiotic bacteria planting and seed metabolism are promoted. The foliar fertilizer containing the garlic extracting solution, the onion leaching solution, the monopotassium phosphate, the boric acid and the urea is adopted, elements needed by growth of the rhododendron simsii are provided, the strong insecticidal and bactericidal effects are achieved, and therefore the incidence rate of diseases and pests is reduced, and the seedling survival rate and the breeding efficiency of the rhododendron simsii are improved.

Description

Technical Field

[0001] The invention relates to the technical field of azalea orchid breeding, and in particular to an efficient azalea orchid breeding method. Background Art

[0002] Rhododendron orchids are perennial terrestrial herbs of the genus Rhododendron in the Orchidaceae family. Their pseudobulbs contain a variety of chemical substances with significant medicinal value, including anticancer and antibacterial properties. They are primarily used in medicine to treat carbuncle, furuncle, scrofula, lymphadenopathy, and snake and insect bites. To date, compounds with structures such as phenanthrenes, dihydrophenanthrenes, bibenzyls, dimeric phenanthrenes, and phenanthrenquinones have been extracted from rhododendron orchids. Rhododendron orchids are primarily used as medicinal plants. Furthermore, they possess the high ornamental value of orchids, resulting in high comprehensive application value and widespread popularity. Rhododendron orchids also exhibit biological characteristics common to orchids. In natural environments, their inflorescences produce very low fruit and pods, their seeds are prone to abortion, and seed germination is difficult. Consequently, naturally grown rhododendron orchids have an extremely low reproductive coefficient, relying primarily on pseudobulb division for reproduction, resulting in a small number of wild rhododendron orchids.

[0003] Wild azalea orchids have a very low self-propagation coefficient, with a fruiting rate of only 1.3% to 2.0% under natural conditions, and seeds have difficulty germinating naturally. To protect wild azalea orchid resources and maintain their medicinal value, artificial cultivation is currently the preferred method. Summary of the Invention

[0004] In view of this, the purpose of the present invention is to reduce the incidence of diseases and pests of azalea orchids, and to increase the survival rate after transplanting, so as to achieve efficient breeding.

[0005] In order to achieve the above effects, the present invention provides the following technical solutions:

[0006] An efficient breeding method for azalea orchids comprises the following steps:

[0007] (1) Site preparation and selection: In February of the lunar calendar each year, select a site with sandy loam under the forest at an altitude of 800-2000 m, a canopy density of 0.55-0.75, slightly acidic soil, and rich humus. Dig ridges with a height of 30-40 cm and a width of 1 m. At the same time, dig drainage ditches to prevent water accumulation.

[0008] (2) Spreading the fungus: Sprinkle a first layer of high-temperature sterilized shredded wood leaves or dead branches and leaves on the box surface, then spread the azalea orchid germination fungus culture spawn on the above shredded wood leaves or dead branches and leaves, and then spread a second layer of high-temperature sterilized shredded wood leaves or dead branches and leaves to cover the fungus;

[0009] (3) Laying wood: Place fresh hardwood closely on the spawn of shredded wood leaves or dead branches and leaves;

[0010] (4) Filling the gaps: Spread the humus soil containing the composite microbial agent into the gaps between the wood;

[0011] (5) Seed mixing: Mix sawdust, perlite, coconut bran, decomposed organic fertilizer, bamboo charcoal powder, wood vinegar, water and seeds evenly;

[0012] (6) Sowing: Sprinkle the mixed seeds evenly on the filled wood, and then lay humus soil and pine needles in turn;

[0013] (7) Shading: After sowing, put up a shade net with a density of 70% to 80% 2m above the ground.

[0014] (8) Management: Regularly weed and apply fertilizer, and maintain the soil humidity at 55% to 65% in the later stage.

[0015] Preferably, the pH value of the soil in step (1) is 5-7.

[0016] Preferably, the method for high-temperature disinfection of the chopped wood leaves or dead branches and leaves in step (2) is: boiling the chopped wood leaves or dead branches and leaves in boiling water for 30 to 40 minutes, cooling them, removing them, drying them, and setting them aside.

[0017] Preferably, in step (2), the thickness of the first and second layers of shredded wood leaves or dead branches and leaves is 2 to 3 cm; the particle size of the azalea orchid germination fungus cultivation strain is 2 to 3 cm, and the spacing is 2 to 3 cm.

[0018] Preferably, the broadleaf wood in step (3) has a diameter of 3 to 6 cm and a length of 15 to 30 cm.

[0019] Preferably, the composite microbial agent in step (4) comprises the following raw materials in parts by weight:

[0020] 1-3 parts of Bacillus subtilis, 1-3 parts of Bacillus licheniformis, and 3-5 parts of Paenibacillus gelatinosa.

[0021] Preferably, the addition amount of the composite microbial agent in step (4) is 5% to 10% of the mass of the humus soil.

[0022] Preferably, the mass ratio of the sawdust, perlite, coconut bran, decomposed organic fertilizer, bamboo charcoal powder, wood vinegar, water and seeds in step (5) is 20-40:10-20:10-20:5-10:5-8:0.05-0.1:10-20:0.06-0.2;

[0023] Preferably, the thickness of the humus soil in step (6) is 3 to 4 cm; the thickness of the pine needles is 3 to 5 cm.

[0024] Preferably, the topdressing in step (8) is to apply foliar fertilizer once every 20 days after 60% to 70% of the seedlings have emerged, for three consecutive times;

[0025] The foliage fertilizer is composed of the following raw materials in parts by weight: 2-3 parts of garlic extract, 2-5 parts of onion extract, 3-5 parts of potassium dihydrogen phosphate, 0.1-0.2 parts of boric acid, 1-3 parts of urea and 80-100 parts of water.

[0026] Compared with the prior art, the present invention has the following beneficial effects:

[0027] The present invention provides an efficient breeding method for azalea orchids, comprising the steps of land preparation and selection, spreading fungus seeds, laying wood, filling gaps, seed mixing, sowing, shading, and management. The present invention adjusts the specific method, using humus soil containing a composite microbial agent to fill the gaps between the wood, and then sowing, thereby improving the germination rate of the seeds. The present invention uses sawdust, perlite, coconut husks, decomposed organic fertilizers, bamboo charcoal powder, and wood vinegar to mix the seeds before sowing. The perlite can improve the air permeability of the substrate, prevent water accumulation and seed rot, and buffer the evaporation rate of water. The bamboo charcoal powder can absorb toxins, adjust pH, and inhibit the reproduction of pathogens. The coconut husks can increase the water retention of the substrate, balance the loose structure of the sawdust, and provide a small amount of potassium. The decomposed organic fertilizer provides humic acid, organic nitrogen, and trace elements to promote the colonization of symbiotic bacteria. The wood vinegar is a natural antibacterial agent (containing phenols and organic acids) that can inhibit mold and soil-borne diseases and promote seed metabolism. The present invention applies foliar fertilizer containing garlic extract, onion extract, potassium dihydrogen phosphate, boric acid and urea after 60% to 70% of the seedlings emerge, which not only provides elements required for the growth of azalea orchids, but also has strong insecticide and fungicide effects, thereby improving the disease and pest resistance rate of azalea orchids. DETAILED DESCRIPTION

[0028] The present invention provides an efficient breeding method for azalea orchid, comprising the following steps:

[0029] (1) Site preparation and selection: In February of the lunar calendar each year, select a forest with sandy loam at an altitude of 800-2000 m, a canopy density of 0.55-0.75, slightly acidic soil (pH 5-7), and rich humus. Dig ridges with a height of 30-40 cm and a width of 1 m. At the same time, dig drainage ditches to prevent water accumulation.

[0030] (2) Spreading the fungus: Boil the chopped wood leaves or dead branches and leaves in boiling water for 30 to 40 minutes, cool them down, remove them and dry them for later use. Sprinkle a first layer of high-temperature sterilized chopped wood leaves or dead branches and leaves (thickness of 2 to 3 cm) on the floor, then spread the azalea orchid germination fungus culture fungus (the particle size of the fungus is 2 to 3 cm, and the interval is 2 to 3 cm) on the chopped wood leaves or dead branches and leaves, and then spread a second layer of high-temperature sterilized chopped wood leaves or dead branches and leaves (thickness of 2 to 3 cm) to cover the fungus.

[0031] (3) Wood laying: Cut fresh broadleaf wood (birch) into wood segments with a diameter of 3 to 6 cm and a length of 15 to 30 cm, and then arrange them closely on the spawn covered with chopped wood leaves or dead branches and leaves;

[0032] (4) Filling the gaps between the wood pieces with humus soil containing a composite microbial agent; the composite microbial agent is composed of the following raw materials in parts by weight: 1 to 3 parts of Bacillus subtilis, 1 to 3 parts of Bacillus licheniformis, and 3 to 5 parts of Paenibacillus jelly;

[0033] The addition amount of the composite microbial agent is 5% to 10% of the mass of the humus soil.

[0034] (5) Seed dressing: Mix sawdust, perlite, coconut bran, decomposed organic fertilizer, bamboo charcoal powder, wood vinegar, water and seeds in a mass ratio of 20-40:10-20:10-20:5-10:5-8:0.05-0.1:10-20:0.06-0.2 and mix evenly;

[0035] (6) Sowing: Sprinkle the mixed seeds evenly on the filled wood, and then lay humus soil with a thickness of 3 to 4 cm and pine needles with a thickness of 3 to 5 cm in sequence;

[0036] (7) Shading: After sowing, put up a shade net with a density of 70% to 80% 2m above the ground.

[0037] (8) Management: Regular weeding and topdressing, and maintaining soil moisture at 55% to 65% in the later stages;

[0038] After 60% to 70% of the seedlings emerge, foliar fertilizer is applied once every 20 days for three consecutive times; the foliar fertilizer is composed of the following raw materials in parts by weight: 2 to 3 parts of garlic extract, 2 to 5 parts of onion extract, 3 to 5 parts of potassium dihydrogen phosphate, 0.1 to 0.2 parts of boric acid, 1 to 3 parts of urea, and 80 to 100 parts of water.

[0039] In a specific embodiment of the present invention, the preparation method of decomposed organic fertilizer is as follows: 6kg of cow dung; 3kg of rice straw; 0.1kg of composite fermentation agent; 0.25kg of humic acid; and 0.25kg of rice bran are weighed respectively; then the cow dung and rice straw are mixed to obtain mixture A; the composite fermentation agent and rice bran are mixed, kept warm at 30°C for 3h, then kept warm at 40°C for 4h, humic acid is added after the insulation is completed, and the mixture is stirred to obtain mixture B; finally, mixture A is mixed with mixture B, the water content thereof is adjusted to 50%, the compost is fermented for 7 days, and then the compost is turned once every 7 days, repeated 3 times, and then the compost is turned once every 10 days, repeated 3 times, and then placed in a cool, dry and ventilated environment for 2 months to obtain the decomposed organic fertilizer.

[0040] The azalea orchid germination fungus cultivation species of the present invention is a conventional product purchased on the market. In a specific embodiment of the present invention, the azalea orchid germination fungus cultivation species is a fungus associated with Radix Cibotii, provided by the Scientific Research Institute of Baili Rhododendron Management District, Guizhou Province, with a deposit number of CCTCCNO: M20221028.

[0041] The Bacillus subtilis, Bacillus licheniformis, and Paenibacillus jelly are all commercially available products, wherein the effective viable count of Bacillus subtilis is ≥150 million / g; the effective viable count of Bacillus licheniformis is ≥200 million / g; and the effective viable count of Paenibacillus jelly is ≥200 million / g. In a specific embodiment of the present invention, the Bacillus subtilis was purchased from Xi'an Tianhe Pharmaceutical Co., Ltd. under the product model TH-0497; Bacillus licheniformis was purchased from Xi'an Tianhe Pharmaceutical Co., Ltd. under the product model TH-0496; and Paenibacillus jelly is Jinyuyuan Bacillus jelly purchased from Shandong Xinzhuoyuan Biotechnology Co., Ltd. under the product number 20180820.

[0042] In a specific embodiment of the present invention, the preparation method of the garlic extract is as follows: 100g of fresh peeled garlic is taken, mashed into a paste, allowed to stand for 30 minutes, and then immersed in 500mL of water for 12 hours to obtain the garlic extract.

[0043] The onion extract is prepared by: taking a number of onions and putting them into a juicer to extract juice, filtering the obtained juice through a 200-mesh filter to obtain a filtrate and a filter residue; adding twice the amount of water to the filter residue and soaking and extracting for 3 hours, continuing to filter to obtain a filtrate and a filter residue, and combining the two filtrates to obtain the onion extract.

[0044] The present invention will be further described below with reference to the examples. In the specific examples of the present invention, the raw materials are all conventionally purchased raw materials unless otherwise specified, and the methods are all conventional methods in the art unless otherwise specified.

[0045] Example 1

[0046] An efficient breeding method for azalea orchid comprises the following steps:

[0047] (1) Site preparation and selection: In February of the lunar calendar each year, select a site with sandy loam under the forest at an altitude of 1000 m, a canopy density of 0.55, a slightly acidic soil of 5.3, and rich humus. Dig ridges with a height of 35 cm and a width of 1 m. At the same time, dig drainage ditches to prevent water accumulation.

[0048] (2) Spreading the fungus: Boil the chopped wood leaves or dead branches and leaves in boiling water for 40 minutes, cool them down, remove them and dry them for later use. Sprinkle a first layer of high-temperature sterilized chopped wood leaves (3 cm thick) on the floor, then spread the azalea orchid germination fungus culture spawn (the particle size of the spawn is 2-3 cm, and the spacing is 2-3 cm) on the chopped wood leaves, and then spread a second layer of high-temperature sterilized chopped wood leaves (2-3 cm thick) to cover the spawn;

[0049] (3) Wood laying: Cut fresh broadleaf wood into wood segments with a diameter of 3 to 6 cm and a length of 15 to 30 cm, and then arrange them closely on the spawn of shredded wood leaves or dead branches and leaves;

[0050] (4) Filling the gaps between the wood pieces with humus soil containing a composite microbial agent; the composite microbial agent is composed of the following raw materials in parts by weight: 2 parts of Bacillus subtilis, 3 parts of Bacillus licheniformis, and 4 parts of Paenibacillus jelly;

[0051] The addition amount of the composite microbial agent is 5% of the mass of the humus soil;

[0052] (5) Seed dressing: Mix sawdust, perlite, coconut husk, decomposed organic fertilizer, bamboo charcoal powder, wood vinegar, water and seeds in a mass ratio of 35:15:17:8:6:0.1:15:0.15 and mix evenly;

[0053] (6) Sowing: Sprinkle the mixed seeds evenly on the filled wood, and then lay 3.5 cm thick humus soil and 3.5 cm thick pine needles in turn;

[0054] (7) Shading: After sowing, put up a shade net with a density of 75% 2m above the ground.

[0055] (8) Management: Regular weeding and topdressing, and maintaining soil moisture at 55% to 65% in the later stages;

[0056] After 65% of the seedlings emerge, foliar fertilizer is applied once every 20 days for three consecutive times; the foliar fertilizer is composed of the following raw materials in parts by weight: 2 parts of garlic extract, 4 parts of onion extract, 3 parts of potassium dihydrogen phosphate, 0.15 parts of boric acid, 2 parts of urea, and 100 parts of water.

[0057] This method starts to germinate about one month after sowing, and seedlings begin to emerge in 60 to 75 days. After one year of growth, the underground bulbs can reach 30 to 50g and the plant height is 10 to 15cm.

[0058] Example 2

[0059] An efficient breeding method for azalea orchid comprises the following steps:

[0060] Steps (1) to (3) are the same as in Example 1;

[0061] (4) Filling the gaps between the wood pieces with humus soil containing a composite microbial agent; the composite microbial agent is composed of the following raw materials in parts by weight: 2 parts of Bacillus subtilis, 3 parts of Bacillus licheniformis, and 4 parts of Paenibacillus jelly;

[0062] The addition amount of the composite microbial agent is 10% of the mass of the humus soil;

[0063] (5) Seed dressing: Mix sawdust, perlite, coconut bran, decomposed organic fertilizer, bamboo charcoal powder, wood vinegar, water and seeds in a mass ratio of 30:15:15:10:5:0.05:15:0.1 and mix evenly;

[0064] (6) Sowing: Sprinkle the mixed seeds evenly on the filled wood, and then lay 3.5 cm thick humus soil and 3.5 cm thick pine needles in turn;

[0065] (7) Shading: After sowing, put up a shade net with a density of 80% 2m above the ground.

[0066] (8) Management: Regular weeding and topdressing, and maintaining soil moisture at 55% to 65% in the later stages;

[0067] After 65% of the seedlings emerge, foliar fertilizer is applied once every 20 days for three consecutive times; the foliar fertilizer is composed of the following raw materials in parts by weight: 4 parts of garlic extract, 5 parts of onion extract, 3 parts of potassium dihydrogen phosphate, 0.15 parts of boric acid, 3 parts of urea, and 100 parts of water.

[0068] Example 3

[0069] An efficient breeding method for azalea orchid comprises the following steps:

[0070] Steps (1) to (3) are the same as in Example 1;

[0071] (4) Filling the gaps between the wood pieces with humus soil containing a composite microbial agent; the composite microbial agent is composed of the following raw materials in parts by weight: 2 parts of Bacillus subtilis, 3 parts of Bacillus licheniformis, and 4 parts of Paenibacillus jelly;

[0072] The addition amount of the composite microbial agent is 7% of the mass of the humus soil;

[0073] (5) Seed dressing: Mix sawdust, perlite, coconut husk, decomposed organic fertilizer, bamboo charcoal powder, wood vinegar, water and seeds in a mass ratio of 40:15:20:10:5:0.1:10:0.2 and mix evenly;

[0074] (6) Sowing: Sprinkle the mixed seeds evenly on the filled wood, and then lay 3 cm thick humus soil and 4 cm thick pine needles in turn;

[0075] (7) Shading: After sowing, put up a shade net with a density of 70% 2m above the ground.

[0076] (8) Management: Regular weeding and topdressing, and maintaining soil moisture at 55% to 65% in the later stages;

[0077] After 65% of the seedlings emerge, foliar fertilizer is applied once every 20 days for three consecutive times; the foliar fertilizer is composed of the following raw materials in parts by weight: 3 parts of garlic extract, 2 parts of onion extract, 5 parts of potassium dihydrogen phosphate, 0.14 parts of boric acid, 3 parts of urea, and 70 parts of water.

[0078] Comparative Example 1

[0079] An efficient breeding method for azalea orchids, the steps are the same as those in Example 1, except that the addition amount of the composite microbial agent in step (4) of Comparative Example 1 is 12% of the mass of the humus soil.

[0080] The addition amount of the composite microbial agent was changed to explore its effect on seed germination rate. During the experiment, other parameters were the same as in Example 1, with the only difference being the ratio of the addition amount of the composite microbial agent to the humus soil. The results are shown in Table 1.

[0081] Table 1

[0082]

[0083]

[0084] As can be seen from Table 1, adding too much composite microbial agent will inhibit the germination rate.

[0085] Comparative Example 2

[0086] An efficient breeding method for azalea orchids, comprising the same steps as in Example 1, except that no perlite is added during the seed mixing process in step (5) of Comparative Example 2.

[0087] Comparative Example 3

[0088] An efficient breeding method for azalea orchids comprises the same steps as in Example 1, except that no decomposed organic fertilizer is added during the seed mixing process in step (5) of Comparative Example 2.

[0089] Comparative Example 4

[0090] An efficient breeding method for azalea orchids, comprising the same steps as in Example 1, except that bamboo charcoal powder is not added during the seed mixing process in step (5) of Comparative Example 2.

[0091] Comparative Example 5

[0092] An efficient breeding method for azalea orchids, comprising the same steps as in Example 1, except that wood vinegar is not added during the seed dressing process in step (5) of Comparative Example 2.

[0093] The addition amount of wood vinegar was changed to explore its effect on seed germination rate. During the experiment, other parameters were the same as in Example 1, except that the addition amount of wood vinegar was different. The results are shown in Table 2.

[0094] Table 2

[0095] Amount of wood vinegar added (parts) Seed germination rate (%) 0 61 0.05 79 0.08 84 0.1 87 0.12 63 0.15 59 0.2 52

[0096] As can be seen from Table 2, adding too much wood vinegar will inhibit the germination rate.

[0097] Comparative Example 6

[0098] An efficient breeding method for azalea orchids, comprising the same steps as in Example 1, except that coconut husks are not added during the seed mixing process in step (5) of Comparative Example 2.

[0099] Comparative Example 7

[0100] An efficient breeding method for azalea orchids, comprising the same steps as in Example 1, except that garlic extract is not added to the foliage fertilizer in step (8) of Comparative Example 2.

[0101] Comparative Example 8

[0102] An efficient breeding method for azalea orchids, comprising the same steps as in Example 1, except that onion extract is not added to the foliage fertilizer in step (8) of Comparative Example 2.

[0103] Comparative Example 9

[0104] An efficient breeding method for azalea orchids, comprising the same steps as in Example 1, except that boric acid is not added to the foliage fertilizer in step (8) of Comparative Example 2.

[0105] The results of seed germination rate, seedling survival rate and pest and disease incidence of Rhododendron in Examples 1 to 3 and Comparative Examples 1 to 9 are shown in Table 3.

[0106] Table 3

[0107] Seed germination rate (%) Seedling survival rate (%) Incidence rate of pests and diseases (%) Example 1 87 99.2 5.7 Example 2 84 98.1 3.2 Example 3 91 99.4 4.4 Comparative Example 1 71 91.7 9.8 Comparative Example 2 43 94.4 7.4 Comparative Example 3 57 93.1 9.6 Comparative Example 4 55 94.3 9.9 Comparative Example 5 61 92.2 10.3 Comparative Example 6 58 91.3 8.7 Comparative Example 7 88 88.3 15.7 Comparative Example 8 85 84.5 16.9 Comparative Example 9 89 86.7 13.2

[0108] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. An efficient breeding method for azalea orchid, characterized in that: The following steps are involved: (1) Site preparation and selection: In February of the lunar calendar each year, select a site with sandy loam under the forest at an altitude of 800-2000 m, a canopy density of 0.55-0.75, slightly acidic soil, and rich humus. Dig ridges with a height of 30-40 cm and a width of 1 m. At the same time, dig drainage ditches to prevent water accumulation. (2) Spreading the fungus: Sprinkle a first layer of high-temperature sterilized shredded wood leaves or dead branches and leaves on the box surface, then spread the azalea orchid germination fungus culture spawn on the above shredded wood leaves or dead branches and leaves, and then spread a second layer of high-temperature sterilized shredded wood leaves or dead branches and leaves to cover the fungus; (3) Laying wood: Place fresh hardwood closely on the spawn of shredded wood leaves or dead branches and leaves; (4) Filling the gaps: Spread the humus soil containing the composite microbial agent into the gaps between the wood; (5) Seed mixing: Mix sawdust, perlite, coconut bran, decomposed organic fertilizer, bamboo charcoal powder, wood vinegar, water and seeds evenly; (6) Sowing: Sprinkle the mixed seeds evenly on the filled wood, and then lay humus soil and pine needles in turn; (7) Shading: After sowing, a shade net with a density of 70% to 80% is set up 2m above the ground; (8) Management: Regularly weed and apply fertilizer, and maintain the soil humidity at 55% to 65% in the later stage.

2. The efficient breeding method of azalea orchid according to claim 1, characterized in that: The pH value of the soil in step (1) is 5-7.

3. The efficient breeding method of azalea orchid according to claim 1, characterized in that: The method for high-temperature disinfection of the chopped wood leaves or dead branches and leaves in step (2) is as follows: boil the chopped wood leaves or dead branches and leaves in boiling water for 30 to 40 minutes, cool them, remove them, dry them, and set them aside.

4. The efficient breeding method of azalea orchid according to claim 1, characterized in that: In step (2), the thickness of the first and second layers of chopped wood leaves or dead branches and fallen leaves is 2 to 3 cm; the particle size of the azalea orchid germination fungus cultivation strain is 2 to 3 cm, and the interval is 2 to 3 cm.

5. The efficient breeding method of azalea orchid according to claim 1, characterized in that: The broadleaf wood in step (3) has a diameter of 3 to 6 cm and a length of 15 to 30 cm.

6. The efficient breeding method of azalea orchid according to claim 1, characterized in that: The composite microbial agent in step (4) comprises the following raw materials in parts by weight: 1-3 parts of Bacillus subtilis, 1-3 parts of Bacillus licheniformis, and 3-5 parts of Paenibacillus gelatinosa.

7. The efficient breeding method of azalea orchid according to claim 1, characterized in that: The addition amount of the composite microbial agent in step (4) is 5% to 10% of the mass of the humus soil.

8. The efficient breeding method of azalea orchid according to claim 1, characterized in that: The mass ratio of the sawdust, perlite, coconut chaff, decomposed organic fertilizer, bamboo charcoal powder, wood vinegar, water and seeds in step (5) is 20-40:10-20:10-20:5-10:5-8:0.05-0.1:10-20:0.06-0.

2.

9. The efficient breeding method of azalea orchid according to claim 1, characterized in that: The thickness of the humus soil in step (6) is 3 to 4 cm; the thickness of the pine needles is 3 to 5 cm.

10. The efficient breeding method of azalea orchid according to claim 1, characterized in that: The topdressing in step (8) is to apply foliar fertilizer once every 20 days after 60% to 70% of the seedlings have emerged, for three consecutive times; The foliage fertilizer is composed of the following raw materials in parts by weight: 2-3 parts of garlic extract, 2-5 parts of onion extract, 3-5 parts of potassium dihydrogen phosphate, 0.1-0.2 parts of boric acid, 1-3 parts of urea and 80-100 parts of water.

Images