Nutritional composition for bidirectionally regulating and controlling people with constipation and diarrhea based on biological three-dimensional enzymolysis technology and processing technology of nutritional composition

Abstract

The invention discloses a nutritional composition for bidirectionally regulating and controlling people with constipation and diarrhea based on a biological three-dimensional enzymolysis technology. The nutritional composition comprises the following components in parts by mass: 30-40 parts of oat, 20-30 parts of millet, 20-25 parts of highland barley and 10-15 parts of oat fibers. Oat, millet, highland barley and oat fiber are used as enzymolysis base materials, and the oat-millet-highland barley-oat-fiber-containing health-care food is prepared after segmented biological enzymolysis, inactivation and drying. The invention further discloses a processing technology of the nutritional composition for bidirectionally regulating and controlling people suffering from constipation and diarrhea based on the biological three-dimensional enzymolysis technology. The processing technology sequentially comprises the following technological steps of raw material pretreatment, segmented enzymolysis, inactivation and drying. Through raw material compounding and process innovation, bidirectional regulation and control of a single product on constipation / diarrhea are achieved for the first time, drug dependence is avoided, and the traditional Chinese medicine composition is suitable for special crowds for postoperative rehabilitation, IBS and the like.

Description

Technical Field

[0001] The present invention relates to the technical field of functional foods and special medical purpose formula foods, and specifically to a nutritional composition prepared based on biological stereoenzymatic hydrolysis technology and capable of bidirectionally regulating the intestinal function of people suffering from constipation and diarrhea, and a processing technology thereof. Background Art

[0002] 1. Clinical needs for intestinal dysfunction Constipation and diarrhea are common intestinal disorders. Traditional treatments often rely on medications (such as laxatives or antidiarrheals), but long-term use can easily lead to intestinal flora imbalance and drug dependence. Dietary fiber intake can regulate intestinal function in two ways: soluble fiber (such as β-glucan) relieves diarrhea, while insoluble fiber promotes intestinal motility and improves constipation.

[0003] 2. Shortcomings of existing technologies Single dietary fiber products (such as oat bran) are effective for constipation but may worsen diarrhea. Traditional enzymatic hydrolysis processes often target a single raw material (such as highland barley or oats), failing to synergistically optimize the combined functions of multiple cereal fibers.

[0004] Patent publication number CN118696987A: A nutritional composition, preparation method, and application thereof, comprising the following components, by weight: 0.2-5 parts of postbiotics, 0.02-0.5 parts of lactoferrin, and 3-15 parts of inulin; the postbiotics are composed of one or more bacterial cells and metabolites of yeast, lactobacillus, and bifidobacterium; the inulin has a degree of polymerization of 2-60, with an average degree of polymerization of 8-13. By improving the balance of the intestinal microbiome, this composition has a positive impact on intestinal discomfort symptoms such as constipation and diarrhea, but there is still much room for improvement in achieving true bidirectional regulation. Summary of the Invention

[0005] The purpose of the present invention is to overcome the defects in the prior art and provide a nutritional composition for bidirectionally regulating constipation and diarrhea in people based on biological stereoenzymatic hydrolysis technology. The composition uses oats, millet, barley and oat fiber as enzymatic hydrolysis base materials, and achieves high dietary fiber, low GI value and prebiotic function through composite enzymatic hydrolysis process optimization, and is suitable for nutritional intervention of people with intestinal dysfunction.

[0006] To achieve the above objectives, the technical solution of the present invention is to design a nutritional composition for bidirectionally regulating constipation and diarrhea in people based on biological stereoenzymatic hydrolysis technology, comprising 30-40 parts by mass of oats, 20-30 parts by mass of millet, 20-25 parts by mass of highland barley, and 10-15 parts by mass of oat fiber; oats, millet, highland barley and oat fiber are used as enzymatic hydrolysis base materials, and the composition is prepared after segmented biological enzymatic hydrolysis, inactivation and drying.

[0007] A further technical solution is that the GI value of the product after enzymatic hydrolysis is ≤40.

[0008] A further technical solution is that the nutritional composition has a β-glucan content of ≥8%, a slowly digestible starch content of ≥15%, and a total dietary fiber content of ≥25%.

[0009] The present invention also provides a technical solution, which is a processing technology for bidirectionally regulating a nutritional composition for people with constipation and diarrhea based on biological stereoenzymatic hydrolysis technology. The processing of the nutritional composition includes the following process steps in sequence: raw material pretreatment, segmented enzymatic hydrolysis, inactivation and drying.

[0010] A further technical solution is that the raw material pretreatment process is: ultrafine grinding of oats and barley, and puffing of millet.

[0011] A further technical solution is that the particle size of the ultrafinely ground oats and barley is ≤50 μm.

[0012] A further technical solution is that the segmented enzymatic hydrolysis process is a gradient enzymatic hydrolysis process: In the first stage, cellulase and xylanase were used to degrade the cell wall to release β-glucan. The enzymatic hydrolysis temperature was 50°C, and the pH of the hydrolyzate was maintained at 5.5. The second stage: a stereoenzymatic process using pullulanase and α-amylase is used to hydrolyze fast-digestible starch to produce slow-digestible starch; the enzymatic hydrolysis temperature is 60°C, and the pH of the hydrolyzate is maintained at 6.5 during the enzymatic hydrolysis process.

[0013] A further technical solution is that the amount of cellulase and xylanase added is 0.1%, and the ratio of cellulase to xylanase is (0-100):(100-0).

[0014] A further technical solution is that the addition amount of pullulanase and α-amylase is 0.2%, and the ratio of pullulanase to α-amylase is (0-100):(100-0).

[0015] A further technical solution is that the inactivation and drying process is as follows: inactivate the enzyme at 90°C for 10 minutes, and spray dry to obtain powder; wherein, the spray drying parameters are: inlet air temperature 180°C, outlet air 80°C; the moisture content of the powder obtained by drying is ≤5%.

[0016] The advantages and beneficial effects of the present invention are: through raw material compounding and process innovation, the present invention realizes for the first time the bidirectional regulation of constipation / diarrhea with a single product, and has no drug dependence, and is suitable for special populations such as postoperative rehabilitation and IBS. DETAILED DESCRIPTION

[0017] The following examples are only used to illustrate the technical solution of the present invention more clearly, and are not intended to limit the scope of protection of the present invention.

[0018] The present invention is a nutritional composition for bidirectionally regulating constipation and diarrhea based on biological stereoenzymatic hydrolysis technology. 1. Formula components and scientific basis. Formula advantages: - Two-way adjustment mechanism: - People with constipation: Insoluble fiber (oat fiber) increases stool bulk and stimulates peristalsis; slowly digestible barley starch delays gastric emptying.

[0019] - People with diarrhea: The β-glucan in oats and barley forms a gel barrier that absorbs moisture and inhibits pathogens.

[0020] - Nutritional synergy: The zinc and iron in millet and the calcium in barley synergistically enhance the repair of intestinal mucosa.

[0021] 2. Unique processing technology Process flow: 2.1. Raw material pretreatment: - Ultrafine grinding of oats and highland barley (particle size ≤ 50μm), and puffing of millet (to improve starch accessibility).

[0022] 2.2. Segmented enzymatic hydrolysis: - Stage 1 (50°C, pH 5.5): Cellulase + xylanase (0.1% addition) degrade the cell wall and release β-glucan.

[0023] - Second stage (60℃, pH 6.5): Pullulanase + α-amylase (added at 0.2%) hydrolyze rapidly digestible starch (RDS↓20%) to produce slowly digestible starch (SDS).

[0024] 2.3. Inactivation and drying: - Inactivate the enzyme at 90°C for 10 min, and spray dry (inlet air temperature 180°C, outlet air 80°C) to obtain powder (moisture ≤ 5%).

[0025] Process innovation: - Stereoenzymatic hydrolysis: Through temperature / pH gradient regulation, fiber dissolution and starch modification are achieved simultaneously (patented core).

[0026] - Low GI design: The GI value of the enzymatic hydrolysis product is ≤40 (measured value), which is better than that of a single raw material (oat GI = 50-60).

[0027] Nutritional and functional characteristics 5. Clinical value: Functional Verification - Subjects: 100 healthy adults (25-45 years old), 7-day travel test - How to use: Add 30g of this product to your breakfast every day - result: - Intestinal comfort score: 4.5 / 5 points (control group 3.2 points) - Bowel movement regularity: 89% of the subjects maintained 1-2 normal bowel movements per day - 71% reduction in bloating - Constipation model test: Defecation frequency increased by 50% (vs control group), and fecal water content was optimized to 70-75%.

[0028] - Diarrhea model test: Intestinal transit time was prolonged by 40% and pathogens (such as Escherichia coli) were reduced by 60%. In vitro simulated digestion experiment Objective: To verify the stability of the composition and the release pattern of active ingredients in the gastrointestinal environment. Methods: The INFOGEST in vitro digestion model (oral-gastrointestinal three-phase) was used.

[0029] result: in conclusion: - The composition remains stable in the acidic environment of the stomach (β-glucan retention >90%); - Dietary fiber is fully released during the colon phase to support microbial fermentation. Intestinal flora modulation experimental methods: - In vitro fermentation: Healthy human fecal flora was co-cultured with the composition for 24 hours, and SCFAs and flora changes were measured.

[0030] - Key data: (#p<0.05 vs control group) Conclusion: It significantly promotes the proliferation of beneficial bacteria and inhibits potential pathogenic bacteria. Process parameter optimization and verification Methods: The effects of single enzymatic hydrolysis and stereoenzymatic hydrolysis on product function were compared.

[0031] result: Conclusion: Stereoenzymatic hydrolysis significantly increased the yield of functional components (p<0.01). Accelerated stability test Conditions: 40℃ / 75% RH, storage for 6 months.

[0032] result: Conclusion: The product has good stability and meets commercial storage requirements. The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the technical principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. A nutritional composition for bidirectionally regulating constipation and diarrhea based on biological stereoenzymatic hydrolysis technology, characterized in that: The invention comprises 30-40 parts by mass of oats, 20-30 parts by mass of millet, 20-25 parts by mass of highland barley and 10-15 parts by mass of oat fiber. The oats, millet, highland barley and oat fiber are used as enzymatic hydrolysis base materials, and the oats are prepared by segmented bio-enzymatic hydrolysis, inactivation and drying.

2. The nutritional composition for bidirectionally regulating constipation and diarrhea based on biological stereoenzymatic hydrolysis technology according to claim 1, characterized in that: The GI value of the enzymatic hydrolysis product is ≤40.

3. The nutritional composition for bidirectionally regulating constipation and diarrhea based on biological stereoenzymatic hydrolysis technology according to claim 2, characterized in that: The nutritional composition has a β-glucan content of ≥8%, a slowly digestible starch content of ≥15%, and a total dietary fiber content of ≥25%.

4. A process for bidirectionally regulating the nutritional composition for constipation and diarrhea based on biological stereoenzymatic hydrolysis technology, wherein the nutritional composition according to claims 1 to 3 is processed, characterized in that: The method comprises the following process steps in sequence: raw material pretreatment, segmented enzymatic hydrolysis, inactivation and drying.

5. The processing technology for bidirectionally regulating the nutritional composition for constipation and diarrhea patients based on biological stereoenzymatic hydrolysis technology according to claim 4, characterized in that: The raw material pretreatment process comprises the following steps: ultrafine grinding of oats and highland barley, and puffing of millet.

6. The processing technology for bidirectionally regulating the nutritional composition for constipation and diarrhea patients based on biological stereoenzymatic hydrolysis technology according to claim 5, characterized in that: The particle size of ultrafinely ground oats and barley is ≤50μm.

7. The processing technology for bidirectionally regulating the nutritional composition for constipation and diarrhea patients based on biological stereoenzymatic hydrolysis technology according to claim 6, characterized in that: The segmented enzymatic hydrolysis process is a gradient enzymatic hydrolysis process: In the first stage, cellulase and xylanase were used to degrade the cell wall to release β-glucan. The enzymatic hydrolysis temperature was 50°C, and the pH of the hydrolyzate was maintained at 5.

5. The second stage: a stereoenzymatic process using pullulanase and α-amylase is used to hydrolyze fast-digestible starch to produce slow-digestible starch; the enzymatic hydrolysis temperature is 60°C, and the pH of the hydrolyzate is maintained at 6.5 during the enzymatic hydrolysis process.

8. The processing technology for bidirectionally regulating the nutritional composition for constipation and diarrhea patients based on biological stereoenzymatic hydrolysis technology according to claim 7, characterized in that: The addition amount of cellulase and xylanase was 0.1%.

9. The processing technology for bidirectionally regulating the nutritional composition for constipation and diarrhea patients based on biological stereoenzymatic hydrolysis technology according to claim 8, characterized in that: The addition amount of pullulanase and α-amylase was 0.2%.

10. The processing technology for bidirectionally regulating the nutritional composition for constipation and diarrhea patients based on biological stereoenzymatic hydrolysis technology according to claim 9, characterized in that: The inactivation and drying process comprises: inactivating the enzyme at 90° C. for 10 minutes, and spray drying to obtain a powder; wherein the spray drying parameters are: inlet air temperature of 180° C., outlet air temperature of 80° C.; and the moisture content of the powder obtained by drying is ≤5%.