Preparation method and application of prepared rhizoma polygonati extract

Abstract

The invention provides a preparation method and application of a prepared rhizoma polygonati extract, and the preparation method comprises the following steps: mixing 40-100 parts by weight of prepared rhizoma polygonati which is steamed and prepared for nine times and 1-60 parts by weight of peanut shell powder, adding water which is 3-10 times of the mass of the mixture to submerge the mixture, and then heating to carry out reflux extraction; refluxing for 1-10 hours, cooling to 30-50 DEG C, adding 0.001-0.1 part of amylase, and keeping the temperature for 10-50 hours; and heating to 100 DEG C, keeping the temperature for 10-60 minutes, filtering, and taking the filtrate as the prepared rhizoma polygonati extract. By adding the peanut shell powder and improving the existing enzymolysis process, the rhizoma polygonati extracting solution is easier to filter, the solid yield is increased by 10% or above, and the obtained rhizoma polygonati extract is high in effective component content.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicine extraction, and particularly relates to a preparation method and application of a prepared polygonatum sibiricum extract. Background Art

[0002] Polygonatum, also known as tiger ginger and chicken claw ginseng, tonifies qi and nourishes yin, strengthens the spleen, moistens the lungs, and benefits the kidneys. It is used to treat spleen and stomach qi deficiency, fatigue, stomach yin deficiency, dry mouth and poor appetite, lung deficiency and dry cough, hemoptysis caused by overwork, deficiency of essence and blood, soreness of the waist and knees, premature graying of hair, and internal heat and thirst (Chinese Pharmacopoeia). Traditional processing techniques for Polygonatum mainly involve "long steaming and long drying" (Leigong Paozhi Lun) or "nine steaming and nine drying" (Dietary Therapy Materia Medica).

[0003] Raw Polygonatum sibiricum extract has a numbing taste that irritates the throat, can easily cause numbness in the mouth and tongue, and trigger allergic reactions. The nine-steaming and nine-processing process helps increase the effective active ingredients in Polygonatum sibiricum, such as saponins, flavonoids, and polyphenols. The degradation of the polysaccharides in Polygonatum sibiricum significantly increases the reducing sugar content, particularly fructose.

[0004] Polygonatum sibiricum extract is a plant component extracted from the traditional Chinese medicine Polygonatum sibiricum. Currently, the commonly used extraction methods are water decoction or alcohol precipitation. The texture of the processed Polygonatum sibiricum is compact. Direct extraction using traditional methods results in low dissolution efficiency and high viscosity of the extract, making filtration and drying processes difficult and resulting in a poor taste for the Polygonatum sibiricum extract. Enzymatic extraction is commonly used in the prior art to address the problem of low extraction yield. Enzymatic extraction utilizes the high specificity of enzymes to decompose plant cell walls and other tissues to increase the dissolution rate of active ingredients. However, the enzymatic extraction methods in the prior art still cannot solve the viscosity problem caused by glycoproteins and other factors in the extract. In addition, the enzymatic process parameters are demanding, which can easily lead to excessive enzymatic hydrolysis, affecting taste and quality. Summary of the Invention

[0005] In order to solve the problems in the prior art, the present invention provides a preparation method and application of a processed polygonatum extract. By adding peanut shell powder and improving the existing enzymatic hydrolysis process, the polygonatum extract is easier to filter, the solid yield is increased by more than 10%, and the obtained processed polygonatum extract has a high content of effective ingredients.

[0006] The present invention solves the technical problem by adopting the following technical solutions:

[0007] The first object of the present invention is to provide a method for preparing a Polygonatum sibiricum extract, comprising the following steps:

[0008] S1, select nine steaming nine processing parts of polygonatum by weight 40-100 parts, peanut shell powder 1-60 parts, add 3 to 10 times the weight of the mixture of water to cover, and then heat and reflux extraction;

[0009] S2, after refluxing for 1 to 10 hours, cool to 30 to 50°C, add 0.001 to 0.1 parts of amylase, and keep warm for 10 to 50 hours;

[0010] S3. After heating to 100° C. and keeping the temperature for 10 to 60 minutes, filter and take the filtrate as the prepared Polygonatum sibiricum extract.

[0011] Furthermore, 0.02 to 0.2 parts of vitamin C need to be added to the mixture in step S1.

[0012] Furthermore, the vitamin C comes from roxburghii, jujube, sea buckthorn, guava, kiwi, citrus or vitamin C tablets.

[0013] Furthermore, the vitamin C comes from roxburghii.

[0014] Furthermore, the added amount of the roxburghii is 1-10 parts.

[0015] Furthermore, the processed polygonatum is obtained by steaming and processing polygonatum multiflorum or polygonatum yunnanensis nine times.

[0016] Furthermore, 50-80 parts of processed polygonatum sibiricum that has been steamed and processed nine times and 15-45 parts of peanut shell powder are selected, and the mass of water added is 5 to 8 times the mass of the mixture.

[0017] Furthermore, 50-80 parts of processed polygonatum sibiricum that has been steamed and processed nine times, 15-45 parts of peanut shell powder, and 3-5 parts of roxburghii are selected, and the mass of water added is 5 to 8 times the mass of the mixture.

[0018] Preferably, 65-80 parts of processed polygonatum sibiricum, 20-40 parts of peanut shell powder and 3-5 parts of roxburghii are selected.

[0019] Furthermore, the reflux temperature is 95-105° C., and the reflux time is 3-8 h.

[0020] Preferably, the reflux time is 3 to 5 hours.

[0021] Preferably, the temperature during reflux is controlled at 100°C.

[0022] Furthermore, 0.01 to 0.08 parts by weight of amylase is added.

[0023] Furthermore, the amylase is selected from α-amylase, β-amylase, saccharifying enzyme or complex amylase.

[0024] Furthermore, the holding time during enzymatic conversion is 24 to 36 hours.

[0025] Furthermore, during enzymatic hydrolysis, the pH value of the solution is adjusted to 5.0-6.5.

[0026] Furthermore, the peanut shells are crushed to 80-100 meshes and then sieved before mixing.

[0027] Furthermore, the prepared Polygonatum sibiricum is cut into small pieces before reflux extraction.

[0028] Furthermore, the polygonatum extract is concentrated under reduced pressure to prepare polygonatum paste.

[0029] Furthermore, during the reduced pressure concentration, the temperature is 60 to 70° C. and the vacuum degree is -0.08 to -0.10 MPa.

[0030] The second object of the present invention is to provide a prepared polygonatum extract obtained by the above preparation method, wherein the content of polygonatum polysaccharide in the extract is greater than 20%.

[0031] The third object of the present invention is to provide a use of a processed polygonatum extract in the preparation of drugs for prolonging life, beautifying the skin, tonifying the kidneys, improving sleep, resisting fatigue, lowering blood sugar or supplementing calcium.

[0032] Furthermore, the medicine is an oral medicine.

[0033] Furthermore, the medicine is in the form of tablets, capsules, pills, granules or liquid preparations.

[0034] Compared with the prior art, the beneficial technical effects of the present invention are:

[0035] The present invention adds peanut shell powder during preparation. The cellulose in the peanut shell can absorb free starch (dextrin) molecules in the polygonatum, reducing the viscosity of the system, providing a good filter-aiding effect and making the extract clearer. The flavonoids in the peanut shell combine with the polygonatum polysaccharide to form a complex that easily cross-links with the keratin in the stratum corneum, improving the moisturizing effect. The microporous structure of the peanut shell provides attachment sites for amylase, increasing the contact area between the amylase and the raw material, improving the enzymatic hydrolysis efficiency, and increasing the solid yield. In addition, the water-soluble vitamins B6 and B2 in the peanut shell dissolve in the extract, having a good enhancement effect on beauty and anti-aging. The addition of vitamin C gives the processed polygonatum extract a strong antioxidant effect, protecting the components in the peanut shell and polygonatum from oxidation and deterioration. At the same time, the antioxidant component is increased, further improving the cosmetic effect. Rosa roxburghii is the king of vitamin C and is rich in vitamin C. Rosa roxburghii is preferably used as a source of vitamin C. Not only does Rosa roxburghii have a high vitamin C content and good antioxidant properties, but the extract is also rich in dietary fiber, resulting in a good taste of the processed polygonatum extract after extraction.

[0036] The use of amylase in the enzymatic hydrolysis stage significantly impacts clarity and solid yield. Amylase hydrolyzes starch and dextrin, which are composed of glucose, into smaller molecules such as glucose and maltose. This increases starch conversion and clarity, resulting in a higher content of Polygonatum sibiricum polysaccharide than 20%, while significantly boosting solid yield. Furthermore, the oligosaccharides generated by amylase enhance transdermal absorption, significantly improving the extract's moisturizing properties.

[0037] The product obtained by preparing the polygonatum extract has good effects of prolonging life, beautifying, tonifying the kidney, improving sleep, resisting fatigue, lowering blood sugar, supplementing calcium, etc. The preparation method of the invention is simple, the raw materials are easily available, and the product is suitable for industrial production.

[0038] The above description is only an overview of the technical solution of the present invention. In order to more clearly understand the technical means of the present invention, it can be implemented in accordance with the contents of the specification. In order to make the above and other purposes, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are specifically listed below. DETAILED DESCRIPTION

[0039] The technical solutions of the present invention are further described in detail below with reference to specific embodiments. It should be understood that the following embodiments are merely exemplary illustrations and explanations of the present invention and should not be construed as limiting the scope of protection of the present invention. All technologies implemented based on the above content of the present invention are encompassed within the scope of protection that the present invention is intended to protect.

[0040] In addition, unless otherwise specified, various raw materials, reagents, instruments and equipment used in the present invention can be purchased from the market or prepared by existing methods.

[0041] Example 1: Preparation of Polygonatum sibiricum Extract

[0042] The specific steps are as follows:

[0043] 1000 g of processed polygonatum sibiricum that has been steamed and processed nine times, 600 g of peanut shell powder, and 10 g of roxburghii roxburghii are selected, submerged in 16 kg of water, heated to 100° C. and subjected to reflux extraction for 1 hour; cooled to 30° C., 0.01 g of amylase is added, and the mixture is kept warm for 50 hours; then, the mixture is heated to 100° C. and kept warm for 10 minutes, filtered, and an extract is obtained; and the extract is concentrated under reduced pressure to obtain polygonatum sibiricum paste.

[0044] Example 2: Preparation of Polygonatum sibiricum Extract

[0045] The specific steps are as follows:

[0046] 1000 g of processed polygonatum sibiricum, 10 g of peanut shell powder, and 100 g of roxburghii roxburghii were selected, covered with 3.33 kg of water, heated to 100° C. and refluxed for 10 hours; cooled to 50° C., 1 g of amylase was added, and the temperature was kept warm for 50 hours; then, the temperature was raised to 100° C. and kept warm for 60 minutes, filtered, and an extract was obtained; and the extract was concentrated under reduced pressure to obtain polygonatum sibiricum paste.

[0047] Example 3: Preparation of Polygonatum sibiricum Extract

[0048] 1000 g of processed polygonatum sibiricum that has been steamed and processed nine times, 200 g of peanut shell powder, and 30 g of roxburghii roxburghii are selected, submerged with 7.3 kg of water, heated to 100° C. and subjected to reflux extraction for 3 hours; cooled to 40° C., 0.1 g of amylase is added, and the mixture is kept warm for 20 hours; then, the mixture is heated to 100° C. and kept warm for 30 minutes, filtered, and an extract is obtained; the extract is concentrated under reduced pressure to obtain polygonatum sibiricum paste.

[0049] Example 4 Preparation of Polygonatum sibiricum Extract

[0050] Compared with Example 3, this embodiment does not add roxburghii, and the rest of the preparation method is the same as Example 3.

[0051] Experimental Example 1: Comparison of extraction liquid operating parameters

[0052] Comparative Example 1: The operation is exactly the same as in Example 3, without adding peanut shell powder and roxburghii.

[0053] Comparative Example 2: The operation is exactly the same as in Example 3, without adding peanut shell powder.

[0054] Comparative Example 3: The operation is exactly the same as in Example 3, except that the peanut shell powder is replaced with rice hull powder.

[0055] Comparative Example 4: The operation is exactly the same as in Example 3, except that the peanut shell powder is replaced with wheat bran powder.

[0056] Comparative Example 5: The operation was exactly the same as in Example 3, except that amylase was replaced by cellulase.

[0057] Comparative Example 6: The operation was exactly the same as in Example 3, except that amylase was replaced by pectinase.

[0058] Comparative Example 7: The operation is exactly the same as in Example 3, except that amylase is replaced by protease.

[0059] The operating parameters of the samples are shown in Table 1. The experiments were repeated three times.

[0060] The contents of vitamin C, B6 and B2 in the prepared polygonatum sibiricum extracts obtained in the comparative examples and examples were detected by HPLC. The results are shown in Table 2.

[0061] Table 1. Effects of each case on the clarity, yield and filtration speed of the extract

[0062]

[0063]

[0064] Note: Compared with Example 3, Δ P<0.05, ΔΔ P < 0.01, ΔΔΔ P<0.001.

[0065] The results in Table 1 show that: the clarity is highest when treated with amylase and peanut shell powder (Examples 1-4); when treated with amylase but without peanut shell powder, the clarity is significantly reduced and the solid yield is slightly increased (Comparative Examples 1 and 2). It can be seen that the clarity is greatly improved after adding peanut shell powder, and the filtration speed is significantly increased. This is mainly because the cellulose in the peanut shell can absorb the free starch molecules in the polygonatum, reducing the viscosity of the system and having a good filtration-aiding effect. When the peanut shell is replaced with rice husks or wheat husks, the clarity decreases, but not to a significant level (Comparative Examples 3-4); without amylase treatment, the clarity decreases significantly (Comparative Examples 5-6, ΔΔ P < 0.01). When treated with amylase and peanut shell powder, the solid yield was high (Examples 1-4); when treated with amylase and rice husk or wheat bran at the same time, the solid yield remained basically unchanged (Comparative Examples 3-4); when not treated with amylase, the solid yield decreased significantly, reaching a significant level (Comparative Examples 5-7, Δ P < 0.05). When the filtrate was treated with amylase and peanut shell powder, the filtration speed was very fast (Examples 1-4); when the filtrate was treated with amylase without peanut shells or with rice husks or wheat husks, the filtration speed decreased significantly (Comparative Examples 1-4, ΔΔ P < 0.01 and ΔΔΔ P < 0.001); without amylase treatment, the filtration rate was significantly reduced by other enzyme treatments, even when peanut shell powder was used, reaching a significant level (Comparative Examples 5-7, Δ P<0.05).

[0066] Therefore, it can be seen that peanut shell powder has a significant effect on improving filtration speed and clarity, mainly because the cellulose in the peanut shell can absorb the free starch molecules in the polygonatum, reducing the viscosity of the system, having a good filter-aiding effect, and making the extract clearer. Amylase has a significant impact on clarity and solid yield, mainly because amylase hydrolyzes starch and dextrin composed of glucose, hydrolyzing the large molecules "starch" and "dextrin" into small molecules "glucose" and "maltose", etc., improving clarity and significantly increasing the solid yield. However, pectinase and protease, which are commonly used in current enzymatic hydrolysis processes, have poor enzymatic hydrolysis effects on starch in polygonatum and have little effect on filtration efficiency and clarity.

[0067] Table 2. Changes in polysaccharide and vitamin B content in each extract

[0068]

[0069]

[0070] Note: Compared with Example 3, Δ P<0.05, ΔΔ P < 0.01, ΔΔΔ P<0.001.

[0071] The results in Table 2 show that the polysaccharide content of the Polygonatum sibiricum extract in each embodiment and Comparative Examples 1-4 does not change much. Instead of using amylase to replace the sample that other proteins are not processed, the polysaccharide content is obviously on the low side. It can be seen that peanut shell powder has less decomposition effect on starch and dextrin, and amylase plays a major role in the decomposition of starch and dextrin. The change of vitamin C is mainly related to the addition of roxburghii roxburghii. The addition of roxburghii roxburghii can significantly increase the content of vitamin C in the extract. Without adding the extract of roxburghii roxburghii, the content of vitamin C is extremely low (Example 4, Comparative Example 1). The variation trend of vitamin B2 and vitamin B6 is exactly the same: the more peanut shells are added, the more vitamin B2 and B6 are increased (Example 1-3). In Comparative Example 1, the same peanut shell is added, but without adding roxburghii roxburghii, vitamin B2 and B6 content are significantly reduced. This is mainly due to the fact that adding vitamin C in roxburghii roxburghii can protect the loss of vitamin B2 and B6 in the extraction process. Not adding vitamin C causes the vitamin B2 and B6 in the peanut shell powder to be lost in the extraction process, affecting nutritional ingredients in the extract (Example 3 and 4, Comparative Example 1). When peanut shells and rice husks were not added, vitamin C and vitamin B were significantly reduced (Comparative Example 1). Without peanut shells, the vitamin B content decreased regardless of whether roxburghii was added (Example 4, Comparative Examples 1-3). When peanut shells were replaced with rice husks, the vitamin B2 and B6 contents decreased rapidly (Comparative Example 3); when wheat bran was replaced, the vitamin B2 and B6 contents increased significantly (Comparative Example 4); using protease instead of amylase had essentially no effect on the vitamins in the extract (Comparative Examples 5-7).

[0072] Experimental Example 2: Comparison of the skin hydration effects of extracts

[0073] 1. Experimental Materials

[0074] Comparative Example 1: The operation is exactly the same as in Example 3, without adding peanut shell powder and roxburghii.

[0075] Comparative Example 2: The operation is exactly the same as in Example 3, without adding peanut shell powder.

[0076] Comparative Example 3: The operation is exactly the same as in Example 3, except that the peanut shell powder is replaced with rice hull powder.

[0077] Comparative Example 4: The operation is exactly the same as in Example 3, except that the peanut shell powder is replaced with wheat bran powder.

[0078] Comparative Example 5: The operation was exactly the same as in Example 3, except that amylase was replaced by cellulase.

[0079] Comparative Example 6: The operation was exactly the same as in Example 3, except that amylase was replaced by pectinase.

[0080] Comparative Example 7: The operation is exactly the same as in Example 3, except that amylase is replaced by protease.

[0081] 2. Experimental methods

[0082] Experimental animals: 130 male 6-week-old SPF-grade KM mice, weighing 27-29 g, were purchased from Hunan Slake Jingda Experimental Animal Co., Ltd. All mice were housed in an environment with a temperature of (22±1)°C, a relative humidity of 40%-60%, and a light intensity of 12h / 12h alternating light and dark.

[0083] The drug was stirred thoroughly until completely dissolved and stored in a refrigerator at 4°C. KM mice were given free access to water and food every day. After one week of adaptive feeding, they were randomly divided into 12 groups, with 10 mice in each group. One group was a blank group, and the rest were model groups and drug groups. The mice in the model and drug groups were subcutaneously injected with galacturonic acid (500 mg / kg), the model group was orally administered with distilled water, and the drug group was given the drug: 1700 mg / kg. After 60 consecutive days of drug administration, the mice were killed, the back skin was removed, and the skin moisture (Zhong Biluan et al. Study on the anti-aging effect of red croaker fish glue collagen on the skin of D-galactose-induced aging mice. Daily Chemical Industry, 2024, 54(05): 550-557) and subcutaneous Hyp (Zeng Zhiyun et al. Study on the anti-aging effect of golden thread ethanol extract on naturally aging mice. Chinese Medicinal Materials, 2020, 43(05): 1200-1204). The relevant results are shown in Table 3 below.

[0084] Table 3. Results of drug cosmetic experiments

[0085]

[0086] Note: Compared with the blank group, Δ P<0.05, ΔΔ P<0.01; compared with the model group, * P<0.05, ** P < 0.01, *** P<0.001.

[0087] The experimental results in Table 3 show that the water content in the model group decreased significantly compared with the blank group ( ΔP<0.01), Hyp (hydroxyproline, representing subcutaneous collagen) decreased significantly ( ΔΔ P < 0.01), indicating that the model was successful. Compared with the model group, each drug group had the effect of increasing the moisture content and collagen content of the skin. The extracts treated with amylase, peanut shell powder and roxburghii simultaneously had extremely significant effects on delaying skin aging and beautifying (Examples 1-3, ***P < 0.001); the extracts without roxburghii treatment had a reduced beautifying effect (Example 4); the extracts treated with amylase without peanut shells or roxburghii had further reduced anti-aging and beautifying effects (Comparative Example 1); the extracts of Polygonatum sibiricum treated without peanut shells or with rice husks or wheat peels had a beautifying effect, but were lower than the extracts treated with peanut shells (Comparative Examples 2-4, **P < 0.01); the extracts treated with peanut shell powder and roxburghii had a very obvious beautifying effect regardless of which enzyme was used (Comparative Examples 5-7, ***P < 0.001). It can be seen that after treatment with peanut shell powder, the flavonoids in the peanut shell and the polygonatum polysaccharide form a complex that is easily cross-linked with the keratin in the stratum corneum, thereby improving the moisturizing effect. At the same time, the oligosaccharides hydrolyzed under the action of amylase enhance the transdermal absorption rate and significantly improve the skin moisturizing effect of the extract. The vitamin C in the roxburghii roxburghii protects the beauty ingredients (vitamin B6 and B2) from being lost during the extraction process, and the anti-aging and beauty effects are further improved. In Example 4, no roxburghii was added, and the moisture content in the skin decreased to a certain extent compared with the other examples, mainly because the vitamins B6 and B2 in the peanut shell were lost to a certain extent during the extraction process.

[0088] Experimental Example 3: Comparison of the Anti-Aging Effects of Extracts

[0089] 1. Test materials

[0090] Comparative Example 1: The operation is exactly the same as in Example 3, without adding peanut shell powder and roxburghii.

[0091] Comparative Example 2: The operation is exactly the same as in Example 3, without adding peanut shell powder.

[0092] Comparative Example 3: The operation is exactly the same as in Example 3, except that the peanut shell powder is replaced with rice hull powder.

[0093] Comparative Example 4: The operation is exactly the same as in Example 3, except that the peanut shell powder is replaced with wheat bran powder.

[0094] Comparative Example 5: The operation was exactly the same as in Example 3, except that amylase was replaced by cellulase.

[0095] Comparative Example 6: The operation was exactly the same as in Example 3, except that amylase was replaced by pectinase.

[0096] Comparative Example 7: The operation is exactly the same as in Example 3, except that amylase is replaced by protease.

[0097] 2. Experimental methods

[0098] (1) A549 cell culture and MTT assay: 1 ml of frozen cells were taken out of the liquid nitrogen tank and rapidly thawed in a 37°C water bath. The recovered cell suspension was then transferred to a 10 ml centrifuge tube on a clean bench. 4 ml of cell culture medium was added and the tube was centrifuged at 3000 rpm for 10 minutes. The supernatant was discarded, and 3 ml of cell culture medium was added again. The tube was pipetted and mixed thoroughly, and then transferred to a cell culture flask. The tube was cultured in an incubator containing 5% CO2 at 37°C. The basal culture medium for A549 cells was DMEM (containing 10% FBS and 1% penicillin-streptomycin).

[0099] (2) ROS scavenging experiment in cells: First, cells were seeded onto 48-well plates, and the monolayer cells were allowed to adhere for 24 h. They were treated with AAPH at concentrations of 0, 1, 10, 50, and 100 μg / ml for 2, 4, 6, and 8 h, respectively. Three replicate wells were set for each concentration. After treatment, fresh serum-free culture medium was added with 5 mmol / L DCFH-DA 2 μl for 1 h (37°C, 5% CO2). Then, residual DCFH-DA was washed with PBS, and 50 μL cell lysis buffer was added for 30 seconds. The cells were resuspended in 800 μL of PBS buffer, and detected and photographed on a fluorescence spectrophotometer with an excitation wavelength of 485 nm and an emission wavelength of 530 nm.

[0100] After determining the optimal modeling concentration and time of AAPH, the ability of drugs to scavenge ROS was compared based on this model. The specific operation was: after the cells adhered, 150 μL of serum-free culture and 1, 5, and 25 μg / ml (concentrations screened by MTT) of each embodiment and comparative example drug were added for co-culture for 1 hour, and then AAPH was added for co-treatment. The culture medium containing AAPH was removed and replaced with fresh serum-free culture medium and continued to be cultured for 16 hours. Each concentration was repeated 3 times and paralleled 2 times. Finally, 2 μl of fluorescent probe 5mmol / LDCFH-DA was added and incubated for 1 hour (37°C, 5% CO2). The residual DCFH-DA was washed with PBS, and 50 μL of cell lysis buffer was added for lysis and the fluorescence value was measured. This experiment was divided into a blank group, a model group, and a drug group. The blank group did not add drugs and AAPH, and the model group did not add drugs. Other operations were the same.

[0101] 3. Experimental results

[0102] Table 4 Experimental results of drugs scavenging intracellular peroxides

[0103]

[0104] Note: Compared with the blank group, #P<0.01; compared with the model group, *P<0.05, **P<0.01.

[0105] Increased peroxides in the body are a major cause of aging, and removing peroxides from the body has an anti-aging effect. The experimental results in Table 4 show that the peroxides (ROS) in the model group were significantly higher than those in the blank group (##P<0.01), indicating successful modeling. Compared with the model group, the ROS values ​​in each drug group decreased. Samples using amylase and adding peanut shell powder significantly enhanced their ability to scavenge free radicals, reaching a significant level at 5 μg / ml (*P<0.05) and a very significant level at 25 μg / ml (**P<0.01) (Examples 1-3, and Comparative Examples 5-7). Samples without peanut shell powder only reached a significant level at 25 μg / ml (*P<0.05) (Comparative Examples 1-4). In the present invention, the flavonoids in peanut shell powder combine with polygonatum polysaccharides to activate the antioxidant potential of polygonatum polysaccharides, improve the antioxidant properties of the processed polygonatum extract, and enhance its DPPH free radical scavenging ability, thereby significantly increasing its anti-aging effect. The addition of vitamin C had little effect on the antioxidant properties of Polygonatum sibiricum extract.

[0106] The above experimental results show that using amylase and peanut shell powder to treat Polygonatum sibiricum extract can not only significantly improve the clarity of the solution and significantly increase the solid yield, but also greatly increase the filtration speed of the solution, significantly increasing the anti-aging and beauty effects of the extract.

[0107] The serial numbers of the above embodiments of the present invention are for description only and do not represent the advantages or disadvantages of the embodiments.

[0108] The above describes the embodiments of the present invention, but the present invention is not limited to the above specific implementation methods. The above specific implementation methods are merely illustrative and not restrictive. Under the guidance of the present invention, ordinary technicians in this field can also make many forms without departing from the scope of protection of the purpose of the present invention and the claims, which are all protected by the present invention.

Claims

1. A method for preparing a Polygonatum sibiricum extract, characterized in that: The following steps are involved: S1, select nine steaming nine processing parts of polygonatum by weight 40-100 parts, peanut shell powder 1-60 parts, add 3 to 10 times the weight of the mixture of water to cover, and then heat and reflux extraction; S2, after refluxing for 1 to 10 hours, cool to 30 to 50°C, add 0.001 to 0.1 parts of amylase, and keep warm for 10 to 50 hours; S3. After heating to 100° C. and keeping the temperature for 10 to 60 minutes, filter and take the filtrate as the prepared Polygonatum sibiricum extract.

2. The method for preparing a processed polygonatum extract according to claim 1, wherein: 0.02 to 0.2 parts of vitamin C need to be added to the mixture in step S1.

3. The method for preparing a processed polygonatum extract according to claim 2, wherein: The vitamin C comes from roxburghii, jujube, sea buckthorn, guava, kiwi fruit, citrus or vitamin C tablets.

4. The method for preparing a processed polygonatum extract according to claim 1, wherein: The processed polygonatum is obtained by steaming and processing polygonatum multiflorum or polygonatum yunnanensis nine times.

5. The method for preparing a processed polygonatum extract according to claim 1, wherein: 50-80 parts of processed polygonatum sibiricum that has been steamed and processed nine times and 15-45 parts of peanut shell powder are selected, and the mass of water added is 5-8 times the mass of the mixture.

6. The method for preparing a processed polygonatum extract according to claim 3, wherein: 50-80 parts of steamed and processed polygonatum, 15-45 parts of peanut shell powder and 3-5 parts of roxburghii are selected, and the mass of water added is 5-8 times of the mass of the mixture.

7. The method for preparing a processed polygonatum extract according to claim 1, wherein: The amylase is selected from α-amylase, β-amylase, saccharifying enzyme or complex amylase.

8. A processed polygonatum extract obtained by the preparation method according to any one of claims 1 to 7, characterized in that: The content of polygonatum polysaccharide in the extract is greater than 20%.

9. Use of the processed polygonatum extract according to claim 8 in the preparation of drugs for prolonging life, beautifying the skin, tonifying the kidney, improving sleep, resisting fatigue, lowering blood sugar or supplementing calcium.

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