A propagation device and production process for Rhodopseudomonas swampus

Abstract

This application discloses a propagation device and its production process for *Rhodopseudomonas palustris*, relating to the technical field of propagation equipment. The device includes a container body, a light-emitting element, and a switching mechanism. The container body is entirely sealed and hollow internally. Inside the container body are independent propagation and sampling boxes. The propagation box is used for bacterial propagation, and the sampling box is used to collect the propagated strains. The light-emitting element is placed inside the container body, with one end fitted with and covered by a growth hood. A gap is left between the inner wall of the growth hood and the outer wall of the light-emitting element. The side wall of the growth hood is made of a transparent material to allow light to pass through. The switching mechanism drives the light-emitting element and the growth hood to switch positions between the propagation box and the sampling box. A sampling component is positioned above the sampling box on the container body to scrape and collect the bacterial biofilm from the outer wall of the growth hood. This application has the effect of improving the efficiency of strain propagation.

Description

Technical Field

[0001] This application relates to the technical field of propagation equipment, and in particular to a propagation device and production process for a *Rhodopseudomonas swampus* agent. Background Technology

[0002] Rhodopseudomonas palustris is a photosynthetic bacterium belonging to the family Rhodospirilluceae and the genus Rhodopseudomonas. Rhodopseudomonas palustris has various uses, including water purification, wastewater treatment, as a feed additive, and as a highly effective bioactive fertilizer in agricultural production. Rhodopseudomonas palustris agents are biological preparations made primarily from this bacterium, used in all of these applications.

[0003] In the production process of Rhodopseudomonas palustris agent, the Rhodopseudomonas palustris strain needs to be propagated first. In addition to artificially cultivating the bacteria in large quantities to ensure sufficient bacterial volume for preparing the agent, the strain also needs to be quality controlled through the propagation process to ensure that the produced Rhodopseudomonas palustris has good activity and stability. At the same time, propagation under controlled environment can effectively avoid contamination by other microorganisms and ensure that the target strain is obtained.

[0004] Because *Rhodopseudomonas palustris* is a photosynthetic bacterium, it requires certain light conditions for propagation. Conventional propagation equipment for this type of bacteria typically includes an external light source and a transparent outer shell to allow light to pass through (such as glass or plastic) into the culture medium. However, due to the phototaxis of *Rhodopseudomonas palustris*, it tends to grow intensively on the inner wall of the outer shell near the light source, forming a biofilm. This not only reduces the effective culture volume of the bacteria but may also lead to decreased mass transfer efficiency, affecting the final propagation result. Summary of the Invention

[0005] To address the issue of bacteria easily concentrating and forming biofilms on the inner wall of the equipment near the light source, thus affecting the final propagation effect, this application provides a propagation device and production process for Rhodopseudomonas palustris agent.

[0006] On the one hand, this application provides a propagation device for Rhodopseudomonas palustris, which adopts the following technical solution:

[0007] A propagation device for Rhodopseudomonas swampus, comprising:

[0008] The container body is completely sealed and hollow inside. Inside the container body are two independent propagation boxes and sampling boxes. The propagation box is used for bacterial propagation, and the sampling box is used to collect the propagated strains.

[0009] The light-emitting element is placed inside the container body. One end of the light-emitting element is fitted with and covered by a growth hood. A gap is left between the inner wall of the growth hood and the outer wall of the light-emitting element. The side wall of the growth hood is made of transparent material to allow light to pass through.

[0010] The switching mechanism is used to drive the light-emitting element and the growth cover to switch positions between the propagation box and the sampling box. The container body is provided with a sampling component above the sampling box to scrape and collect the bacterial biofilm on the outer wall of the growth cover.

[0011] Optionally, the conversion mechanism includes a conversion turntable, which is rotatably connected to the container body with a horizontal axis of rotation. The conversion turntable is sealed to the container body. The light-emitting element is eccentrically placed on the inner wall of the conversion turntable and perpendicular to the side wall of the conversion turntable. The propagation box and the sampling box are arranged side by side at intervals and are at the same height. The light-emitting element switches positions between the propagation box and the sampling box as the conversion turntable rotates. When the light-emitting element rotates to the highest position, it is located above the propagation box and the sampling box.

[0012] Optionally, a circulating turntable is rotatably and sealed to the conversion turntable. The circulating turntable is eccentrically positioned on the conversion turntable, and its rotation axis is parallel to the axis of the conversion turntable. Multiple light-emitting elements are provided, all located on the inner sidewall of the circulating turntable. The multiple light-emitting elements are parallel to each other and concentrically spaced. A driving component is also provided on the inner sidewall of the circulating turntable to drive all light-emitting elements and their corresponding growth covers to rotate on their own axes and revolve around the axis of the conversion turntable.

[0013] Optionally, the light-emitting element is configured as a self-emitting element with its own power supply.

[0014] Optionally, a detection box is provided inside the container body between the propagation box and the sampling box, and a detection camera is provided above the corresponding detection box on the container body, with the detection camera facing the axis of the conversion turntable. A control unit is also provided inside the container body, and the detection camera is electrically connected to the control unit.

[0015] Optionally, the sampling component includes a scraper and a collection box. The scraper is inclined and located above the collection box. The collection box is horizontally slidably connected to the container body and is positioned higher than the sampling box. When the scraper slides with the collection box above the sampling box, the end of the scraper contacts the outer wall of the growth hood and scrapes the bacterial biofilm on the outer wall of the growth hood into the collection box.

[0016] Optionally, the growth hood is coaxially fixed with positioning rings at both ends along its own axis. The outer diameter of the positioning rings is larger than the outer diameter of the side wall of the growth hood, so as to form a recessed positioning groove on the side wall of the growth hood. The shape of the scraper near the end of the growth hood matches the positioning groove.

[0017] Optionally, the two positioning rings are provided with magnetic rings on opposite sides. The magnetic rings are coaxially embedded in the positioning rings and rotatably connected to the positioning rings. Magnetic blocks are provided on both sides of the scraper near the growth cover. After the magnetic blocks and magnetic rings attract each other and are magnetically connected, the end of the scraper contacts the outer wall of the growth cover.

[0018] Optionally, the scraper includes a contact plate, a flexible connector, and a fixed plate. The fixed plate is connected to the collection box. The contact plate is located at one end of the fixed plate near the growth hood and is connected to the fixed plate through the flexible connector. The magnetic blocks are disposed on both sides of the contact plate. The shape of the end of the contact plate matches the positioning groove. The flexible connector itself can deform to allow the contact plate to move with the growth hood to its own limit position.

[0019] On the other hand, this application also provides a production process for Rhodopseudomonas palustris agent, which uses the Rhodopseudomonas palustris agent propagation equipment as described above to prepare the Rhodopseudomonas palustris agent, including the following steps:

[0020] S1: First, add sufficient liquid culture medium to the propagation chamber and add appropriate enzyme solution to the sampling chamber. After adjusting the pH and temperature of the culture medium, inoculate the *Rhodopseudomonas palustris* to be propagated into the propagation chamber, seal the container body for propagation, and then move the growth hood out of the propagation chamber through the conversion mechanism at fixed preset time intervals. Observe the bacterial biofilm on the outer wall of the growth hood. If the bacterial biofilm does not meet the propagation requirements, continue propagation.

[0021] S2: If the bacterial biofilm on the outer wall of the growth hood reaches the propagation requirements, the growth hood will continue to be moved to the sampling box through the conversion mechanism. After the adhesion between the bacteria and the outer wall of the growth hood is weakened by enzymatic hydrolysis, the biofilm on the outer wall of the growth hood will be scraped off and collected by the sampling component. Then the growth hood will be returned to the propagation box through the conversion mechanism.

[0022] S3: Once a certain number of bacteria have been collected in the sampling component, the container body is removed, the collected bacteria are concentrated, and then dried. After drying, the bacteria are tested for viable count and miscellaneous bacteria, and finally sealed and packaged.

[0023] In summary, this application includes at least one of the following beneficial effects:

[0024] 1. By incorporating multiple light-emitting elements within the container body, which are directly placed in the liquid culture medium, and by covering the end of each light-emitting element with a transparent growth cover, the specific wavelength light emitted by the light-emitting elements can uniformly pass through the transparent sidewall of the growth cover and shine into the liquid culture medium. Since *Rhodopseudomonas palustris* is a photosynthetic bacterium, it exhibits phototaxis and is attracted by the specific wavelength light emitted by the light-emitting elements, thus concentrating its growth on the transparent sidewall of the growth cover. Ultimately, a bacterial biofilm is formed on the surface of the growth cover. The bacteria will not grow, or will grow very little, on the inner wall of the container body, but will concentrate on growing on the growth cover based on its phototaxis. Therefore, subsequent collection of the biofilm on the growth cover is sufficient, facilitating the centralized collection of the bacterial strain in the sampling box area, greatly improving the efficiency of bacterial collection and propagation.

[0025] 2. By setting up a detection box between the propagation box and the sampling box, after the bacteria have propagated in the propagation box for a preset time, the growth hood is transferred to the detection box area by the rotation of the turntable. The detection camera in the detection box area takes pictures and uploads the data to the control unit for analysis. Since the side wall of the growth hood with bacterial biofilm attached is located between the light source and the detection camera, the light emitted by the light source passes through the side wall of the growth hood and shines on the detection camera, making it easier for the detection camera to take pictures of the biofilm growth on the outer wall of the growth hood with the help of the light source of the light source. If the propagation is complete, it enters the sampling box area for bacterial collection. If the propagation is not complete, it returns to the propagation box to continue propagation. The entire monitoring process is automatically monitored by the detection camera, without the need for manual inspection, reducing workload.

[0026] 3. By setting a circulating turntable on the conversion turntable, the light-emitting element and the growth hood rotate together with the circulating turntable. The light-emitting element and the growth hood can slowly stir the culture medium in the propagation chamber, so that the bacteria can have more full contact with the culture medium. When the propagation status is detected, the growth hood rotates continuously, so that the detection camera can sample and photograph the biofilm propagation status at different positions on the same growth hood or on different growth hoods, improving the accuracy of the detection. Finally, the growth hood, which can rotate on its own axis and revolve around the sun, makes it easy for the scraper to completely scrape off the biofilm on its surface. Attached Figure Description

[0027] Figure 1 This is a schematic diagram of the overall structure of the propagation device shown in Embodiment 1 of this application;

[0028] Figure 2 This is a schematic diagram of the internal structure of the propagation device shown in Embodiment 1 of this application;

[0029] Figure 3 This is a partial structural schematic diagram of the circulating turntable shown in Embodiment 1 of this application;

[0030] Figure 4This is a partial schematic diagram illustrating the working principle of the scraper in Embodiment 1 of this application;

[0031] Figure 5 This is a partial schematic diagram illustrating the scraper structure in Embodiment 1 of this application;

[0032] Figure 6 yes Figure 5 An enlarged view at point A.

[0033] Explanation of reference numerals in the attached drawings: 1. Container body; 11. Support; 12. Inspection cover; 13. Control unit; 14. Conversion turntable; 141. First driven gear; 15. First drive gear; 16. Circulation turntable; 161. Second drive gear; 162. Second driven gear; 163. Internal gear ring; 2. Propagation box; 3. Detection box; 31. Detection camera; 4. Sampling box; 41. Scraper; 411. Contact plate; 412. Flexible connector; 413. Fixing plate; 414. Magnetic block; 42. Collection box; 421. Rack; 422. Third drive gear; 5. Light-emitting element; 51. Growth cover; 52. Positioning ring; 521. Magnetic ring; 53. Positioning groove. Detailed Implementation

[0034] The following is in conjunction with the appendix Figure 1-6 This application will be described in further detail. Example 1

[0035] Example 1 of this application discloses a propagation device for Rhodopseudomonas palustris, referring to... Figure 1 and Figure 2 The propagation equipment for *Rhodopseudomonas palustris* agent includes a container body 1. The container body 1 is hollow inside, capable of holding the liquid culture medium required for bacterial growth. The container body 1 is entirely sealed, providing a good enclosed environment for bacterial growth and preventing the influence of other microorganisms. A support 11 is fixed to the bottom of the container body 1 for stable placement. An inspection cover 12 is hinged to the top of the container body 1. In operation, the inspection cover 12 is sealed to the container body 1. When it is necessary to inspect or replace internal components of the container body 1, personnel can open the inspection cover 12 for inspection.

[0036] The container body 1 contains a propagation chamber 2, a detection chamber 3, and a sampling chamber 4, all fixed side-by-side at the same height. The internal cavities of the three chambers are independent, with the detection chamber 3 located between the propagation chamber 2 and the sampling chamber 4. The propagation chamber 2 contains liquid culture medium for propagating *Rhodopseudomonas palustris*. The detection chamber 3 is initially empty. The sampling chamber 4 contains a specific enzyme solution, such as protease or cellulase. This specific enzyme degrades the matrix components of the *Rhodopseudomonas palustris* biofilm, thereby weakening the adhesion between the bacteria and the growth medium and facilitating biofilm detachment. The type of enzyme can be determined in preliminary experiments; it can be a single enzyme solution or a solution combining multiple enzymes, depending on the results of previous experiments. The bacteria are first propagated in the propagation chamber 2, then transferred to the corresponding space above the detection chamber 3 for observation. Based on the propagation results, a decision is made whether to continue propagation or transfer the bacteria to the sampling chamber 4 for sampling.

[0037] Furthermore, to facilitate bacterial growth and sampling, a light-emitting element 5 is provided inside the container body 1. Since *Rhodopseudomonas palustris* is a photosynthetic bacterium, it exhibits phototaxis during propagation, growing towards areas with concentrated light sources. In this embodiment, the light-emitting element 5 is configured as a self-powered, self-illuminating element, such as an adjustable-spectrum LED lamp with a built-in battery, allowing it to be placed independently inside the container body 1 without a power cord. The light-emitting element 5 has a cylindrical shape. The light-emitting element 5 can adjust the wavelength of its emitted light, such as red light and near-infrared light, promoting efficient propagation of *Rhodopseudomonas palustris*. A control unit 13 is fixed to the bottom of the container body 1 and the three chambers, including the propagation chamber 2. The control unit 13 can be a PLC control unit with a built-in processing chip. The control unit 13 is electrically connected to the light-emitting element 5, enabling it to automatically adjust the spectrum of the light-emitting element 5.

[0038] To provide an attachment medium for bacterial growth, a growth cover 51 is coaxially fitted and enclosed at one end of the light-emitting element 5. A gap is left between the inner wall of the growth cover 51 and the outer wall of the light-emitting element 5 to facilitate the passage of light emitted by the light-emitting element 5 through the growth cover 51, and also to facilitate the assembly and disassembly of the growth cover 51 from the outside of the light-emitting element 5. The growth cover 51 also has a cylindrical shape, and the part of the light-emitting element 5 that emits light is located inside the growth cover 51. The two ends of the growth cover 51 along its own axis are made of opaque material, such as opaque plastic or metal, while the side walls of the growth cover 51 other than the two ends are made of transparent material, such as transparent glass, allowing light emitted by the light-emitting element 5 to pass through.

[0039] During propagation, the light-emitting element 5, together with the growth cover 51, is placed directly in the liquid culture medium. The specific wavelength light emitted by the light-emitting element 5 can evenly pass through the transparent side wall of the growth cover 51 and shine into the liquid culture medium. Since Rhodopseudomonas palustris is a photosynthetic bacterium, it has a certain phototaxis and will be attracted by the specific wavelength light emitted by the light-emitting element 5, thus concentrating its growth on the transparent side wall of the growth cover 51. Finally, a bacterial biofilm is formed on the surface of the growth cover 51. The bacteria will not grow or will grow very little on the inner wall of the container body 1, but will concentrate on growing on the growth cover 51 based on its own phototaxis. Therefore, it is only necessary to collect the biofilm on the growth cover 51 in the future, which facilitates the concentrated collection of the bacterial strain in the sampling box 4 area, greatly improving the collection and propagation efficiency of bacteria.

[0040] Furthermore, to enable the transfer of strains within various areas for propagation, detection, and sampling, a conversion turntable 14 is rotatably connected to one side wall of the container body 1. The axis of rotation of the conversion turntable 14 is horizontal and corresponds to the position of the detection box 3. The conversion turntable 14 is also sealed to the container body 1. A first driven gear 141 is fixed to the outer wall of the conversion turntable 14, and a first driving gear 15 is rotatably connected to the side wall of the container body 1 corresponding to the conversion turntable 14. The first driving gear 15 meshes with the first driven gear 141. A drive motor is also fixed to the outer wall of the container body 1. The output shaft of the drive motor is coaxially fixed to the first driving gear 15, enabling the first driving gear 15 to rotate.

[0041] A circulating turntable 16 is rotatably and sealed to the conversion turntable 14. The circulating turntable 16 is eccentrically positioned on the conversion turntable 14, and its rotation axis is parallel to the axis of the conversion turntable 14. The circulating turntable 16 can rotate with the conversion turntable 14. The light-emitting element 5 and the growth cover 51 are both set on the inner side wall of the circulating turntable 16, thereby realizing the switching of the position of the growth cover 51 between the propagation box 2, the detection box 3, and the sampling box 4. The specific eccentric position of the circulating turntable 16 can be determined according to the actual size of the conversion turntable 14 and the three boxes, as long as the circulating turntable 16 can just fall into the propagation box 2 or the sampling box 4 under the drive of the conversion turntable 14.

[0042] Furthermore, refer to Figures 1 to 3To ensure that the light-emitting element 5, together with the growth cover 51, can be agitated within the culture medium, a plurality of light-emitting elements 5 are correspondingly arranged on the circulating turntable 16. In Embodiment 1 of this application, three light-emitting elements 5 are preferably arranged, with their axes parallel and all parallel to the rotation axis of the conversion turntable 14. A second driving gear 161 and three second driven gears 162 rotate on the inner sidewall of the circulating turntable 16, respectively. The second driving gear 161 is coaxially arranged with the circulating turntable 16, and the three second driven gears 162 are evenly distributed around the outer periphery of the second driving gear 161 and mesh with the second driving gear 161, forming a planetary gear system. That is, while the three second driven gears rotate on their own axes, they also revolve around the second driving gear 161. An internal gear ring 163 is provided on the outer periphery of the second driven gear 162. The internal gear ring 163 meshes with three second driven gears 162 simultaneously. The internal gear ring 163 provides stable support and guidance for the rotation of the second driven gears 162. The internal gear ring 163 can be directly fixed on the inner wall of the conversion turntable 14. The drive motor provided on the conversion turntable 14 directly drives the second drive gear 161 to rotate. The rotating second drive gear 161 drives the three meshing second driven gears 162 to rotate. Since the second driven gears 162 mesh with the internal gear ring 163 simultaneously, the three second driven gears 162 revolve around the second drive gear 161.

[0043] The light-emitting element 5 corresponds one-to-one with the second driven gear 162. One end of the light-emitting element 5 is fixedly connected to its corresponding second driven gear 162 through a connecting cylinder, while the other end is suspended, allowing the light-emitting element 5 to rotate and revolve with the second driven gear 162. When the light-emitting element 5 and the growth cover 51 are in the propagation chamber 2 for propagation, if left to stand for a long time, the nutrients in the liquid culture medium will slowly settle down. After the nutrients settle down, the bacteria will concentrate on the lower half of the outer wall of the growth cover 51. Therefore, the slowly revolving light-emitting element 5 and the growth cover 51 can slowly and fully agitate the culture medium, making the distribution of nutrients in the liquid culture medium more uniform. At the same time, the revolving light-emitting element 5 and the growth cover 51 can also make the bacteria on the surface of the growth cover 51 more active during the agitation of the liquid culture medium, and can also make the bacteria more fully contact the culture medium. The rotation of the light-emitting element 5 and the growth cover 51 is beneficial for the subsequent scraping and collection of bacteria on the surface of the growth cover 51.

[0044] Furthermore, refer to Figures 2 to 4Inside the container body 1, above the corresponding detection box 3, a detection camera 31 is fixed. The detection camera 31 faces the axis of the conversion turntable 14. When the light-emitting element 5 and the growth cover 51 rotate to the highest position with the circulation turntable 16, the light-emitting element 5 is positioned above the detection box 3. At this time, the detection box 3 can receive the culture medium dripping from the surface of the growth cover 51, and the detection camera 31 can take pictures of the bacterial growth on the surface of the growth cover 51. The detection camera 31 is electrically connected to the control unit 13. The detection camera 31 takes pictures of the surface of the growth cover 51 at specified time intervals, generating raw image data (such as RAW or BMP format). The images are transmitted to the control unit 13 via USB / Ethernet / CameraLink, etc. The control unit 13 performs image preprocessing, including grayscale conversion, noise removal, brightness or contrast adjustment, and image registration. Then, image enhancement and feature extraction are performed to make the traces of bacterial growth more obvious, such as edge enhancement (Canny edge detection, highlighting colony edges) and texture analysis (using GLCM, L...). Methods such as backpropagation (BP) are used to determine surface texture changes, or brightness analysis is performed (detecting bright spots, color patches, grayscale changes, etc. in the image). Then, image segmentation is performed to separate the "colony region" from the "background." Data statistics and calculations are then performed to convert the "features" in the image into "numerical data," such as calculating the area (number of pixels), location (center coordinates), and shape (roundness, aspect ratio) of each colony. Finally, the current image data is compared with the initial image to analyze the bacterial growth trend. If a certain indicator exceeds a set threshold (e.g., colony count > 100), an alarm is triggered or a corresponding control signal is output. Alternatively, the percentage of biofilm coverage per unit area can be analyzed to determine the colony's growth. The detection method is not unique; any method that can identify and assess the biofilm's proliferation is acceptable.

[0045] After bacteria have multiplied in the propagation chamber 2 for a preset time, the growth hood 51 is transferred to the detection chamber 3 area by the rotation of the transfer turntable 14. The detection camera 31 in the detection chamber 3 takes pictures and uploads the data to the control unit 13 for analysis. Since the sidewall of the growth hood 51 with the bacterial biofilm is located between the light-emitting element 5 and the detection camera 31, the light emitted by the light-emitting element 5 passes through the sidewall of the growth hood 51 and is directed to the detection camera 31. This makes it easier for the detection camera 31 to photograph the biofilm growth on the outer wall of the growth hood 51 using the light source of the light-emitting element 5. If propagation is complete, the bacteria enter the sampling chamber 4 area for bacterial collection; if propagation is incomplete, the bacteria return to the propagation chamber 2 to continue propagation. The entire monitoring process is automatically monitored by the detection camera 31, eliminating the need for manual inspection and effectively reducing workload. Simultaneously, during propagation detection, each growth hood 51 rotates on its own axis and revolves around a central point, allowing the detection camera 31 to sample and photograph different locations on the same growth hood 51 or on different growth hoods 51, improving detection accuracy.

[0046] Furthermore, to facilitate the centralized collection of bacterial biofilm on the surface of the growth cover 51, the sampling box 4 contains a specific enzyme solution, which weakens the adhesion between bacteria and the growth cover 51, facilitating biofilm detachment. This has been specifically described above and will not be repeated here. Simultaneously, the container body 1 is equipped with a sampling component above the corresponding sampling box 4 for sampling and collecting the biofilm on the surface of the growth cover 51. Specifically, the sampling component includes a scraper 41 and a collection box 42. The collection box 42 is placed horizontally and slidably connected within the container body 1, while the scraper 41 is tilted above the collection box 42, with the scraper 41 positioned higher near the end of the growth cover 51. A rack 421 is fixed to the bottom of the collection box 42. At the same time, a drive motor is fixed to the container body 1 below the collection box 42. A third drive gear 422 is coaxially fixed on the output shaft of the drive motor. The rotation axis of the third drive gear 422 is horizontal, and the third drive gear 422 meshes with the rack 421. This allows the drive motor to drive the collection box 42 to reciprocate in the horizontal direction towards or away from the growth cover 51 through the meshing third drive gear 422 and rack 421. During this process, the scraper 41 moves synchronously with the collection box 42. The scraper 41 can scrape the biofilm on the surface of the growth cover 51 and drop the biofilm into the collection box 42.

[0047] Furthermore, refer to Figures 4 to 6 Since the growth hood 51 rotates on its own axis while also revolving around a central point, it will revolve at a certain angle when the biofilm is scraped. This angle is determined by the gear ratio between the second drive gear 161 and the second driven gear 162. To ensure that the scraper 41 always maintains contact with the sidewall of the growth hood 51 when scraping the biofilm, positioning rings 52 are coaxially fixed at both ends of the growth hood 51 along its own axis. The outer diameter of the positioning rings 52 is larger than the outer diameter of the sidewall of the growth hood 51, so that the positioning rings 52 at both ends protrude from the growth hood 51, thereby forming recessed positioning grooves 53 with the sidewall of the growth hood 51. Magnetic suction rings 521 are embedded on the two inner surfaces of the two positioning rings 52 respectively. The magnetic suction rings 521 are coaxially arranged with the positioning rings 52 and rotatably connected within the positioning rings 52. In other embodiments of this application, the gear ratio between the second drive gear 161 and the second driven gear 162 can be changed so that when the growth cover 51 completes one rotation, the synchronous revolution angle of the growth cover 51 is as small as possible, so that the scraper 41 can scrape the biofilm on the surface of the growth cover 51.

[0048] The scraper 41 includes a contact plate 411, a flexible connector 412, and a fixing plate 413. The fixing plate 413 is fixedly connected to the collection box 42 via a connecting frame. The contact plate 411 is located at the end of the fixing plate 413 near the growth hood 51 and is connected to the fixing plate 413 via the flexible connector 412. The end of the contact plate 411 can be configured as a wedge shape to better scrape the biofilm on the surface of the growth hood 51. The shape of the end of the contact plate 411 away from the fixing plate 413 matches the positioning groove 53, and a magnetic block 414 is embedded on each side of the contact plate 411. When the end of the contact plate 411 enters the positioning groove 53 and contacts the side wall of the growth hood 51, the magnetic block 414 and the magnetic ring 521 attract each other and are magnetically connected. At this time, the growth hood 51 can continue to rotate, and the magnetic ring 521 and the contact plate 411 remain relatively fixed. The contact plate 411 can scrape the biofilm on the surface of the growth hood 51.

[0049] Since the growth cover 51 rotates on its own axis while also revolving around the sun, in order to further keep the magnetic ring 521 and the contact plate 411 relatively fixed, the flexible connector 412 can be a flexible spring. When the contact plate 411 moves with the growth cover 51, the flexible connector 412 can bend itself to move a certain distance with the contact plate 411, while also maintaining the flexible connection between the contact plate 411 and the fixed plate 413.

[0050] When the contact plate 411 moves with the growth cover 51 to the limit position of the flexible connector 412 through the magnetic connection between the magnetic block 414 and the magnetic ring 521, the flexible connector 412 stops deforming. At this point, the contact plate 411 no longer moves with the growth cover 51 until the magnetic block 414 disengages from the magnetic ring 521. The flexible connector 412 then drives the contact block to return to its initial position, allowing the contact plate 411 to return to its initial position after scraping the biofilm from the surface of one growth cover 51, and continue sampling the next growth cover 51. After the contact plate 411 has scraped the biofilm from the surfaces of all growth covers 51, the scraper 41, together with the collection box 42, returns to its initial position, and the growth cover 51 returns to the propagation box 2 to continue propagation.

[0051] When propagating *Rhodopseudomonas palustris*, appropriate amounts of liquid culture medium and enzyme solution are first added to the propagation chamber 2 and sampling chamber 4, respectively. Then, the rotating turntable 14 rotates, causing the circulating turntable 16 to move towards the propagation chamber 2 until all the corresponding growth hoods 51 inside the propagation chamber 2 are immersed in the liquid culture medium for propagation. At this time, the circulating turntable 16 rotates slowly, causing all the growth hoods 51 to revolve within the culture medium, thoroughly agitating it and ensuring that the bacteria on the outer wall of the growth hoods 51 are in full contact with the culture medium. At preset time intervals, the rotating turntable 14 moves all the growth hoods 51 towards the area of ​​the detection chamber 3. The detection camera 31 monitors the bacterial growth on the surface of all the growth hoods 51. If the bacterial growth density reaches the required level, the bacteria are collected in the area of ​​the sampling chamber 4. During bacterial collection, all growth hoods 51 are first immersed in the enzyme solution in the sampling box 4 to degrade the matrix components in the Rhodopseudomonas palustris biofilm. Then, the turntable 14 drives all growth hoods 51 to detach from the sampling box 4. The collection box 42 extends out and is received below all growth hoods 51. The magnetic block 414 on the contact plate 411 and the magnetic ring 521 on the growth hood 51 are attracted and fixed to each other by magnetic force. At this time, as the growth hood 51 rotates, the bacteria on the outer wall of the growth hood 51 are continuously scraped off by the contact plate. The scraped bacteria fall into the collection box 42 for collection and storage. After all the bacteria on the growth hoods 51 have been scraped off, all the growth hoods 51 continue to enter the propagation chamber 2 under the drive of the conversion turntable 14 for repeated propagation operations. The entire propagation and collection process does not require human monitoring or operation, which greatly reduces the workload of manual operation. At the same time, the bacteria will not grow or will grow very little on the inner wall of the container body 1, but will concentrate on the growth hoods 51 based on their phototaxis. Therefore, it is only necessary to collect the biofilm on the growth hoods 51, which greatly improves the collection and propagation efficiency of bacteria.

[0052] The implementation principle of the propagation device for Rhodopseudomonas palustris agent in Embodiment 1 of this application is as follows: the growth hood 51, together with the light-emitting element 5, is driven by the conversion turntable 14 to switch back and forth between the corresponding areas of the propagation box 2, the detection box 3, and the sampling box 4, so that the growth hood 51 carries out bacterial propagation in the propagation box 2, the propagation status is monitored in the detection box 3 area, and finally the concentrated collection of the strains on the surface of the growth hood 51 is completed in the sampling box 4 area. The light of a specific wavelength emitted by the light-emitting element 5 shines into the culture medium through the transparent side wall of the growth hood 51. Due to phototaxis, Rhodopseudomonas palustris will concentrate on growing on the surface of the growth hood 51. In the subsequent sampling process, the biofilm on the surface of the growth hood 51 is scraped by the contact plate 411 on the scraper 41, and the finally collected strains are concentrated in the collection box 42. Example 2

[0053] Example 2 of this application discloses a production process for Rhodopseudomonas palustris agent, which uses the Rhodopseudomonas palustris agent propagation equipment as described above to prepare the Rhodopseudomonas palustris agent, including the following steps:

[0054] S1: First, add sufficient liquid culture medium to the propagation chamber 2, and at the same time add appropriate enzyme solution to the sampling chamber 4. After adjusting the pH value and temperature of the culture medium, inoculate the *Rhodopseudomonas palustris* to be propagated into the propagation chamber 2, seal the container body 1 for propagation, and then move the growth hood 51 away from the propagation chamber 2 through the conversion turntable 14 at fixed preset time intervals, and observe the bacterial biofilm on the outer wall of the growth hood 51. If the bacterial biofilm does not meet the propagation requirements, continue propagation.

[0055] S2: If the bacterial biofilm on the outer wall of the growth hood 51 reaches the propagation requirements, the growth hood 51 is moved to the sampling box 4 via the transfer turntable 14. After the adhesion between the bacteria and the outer wall of the growth hood 51 is weakened by enzymatic hydrolysis, the biofilm on the outer wall of the growth hood 51 is scraped off and collected by the contact plate 411 on the scraper 41. Then the growth hood 51 is returned to the propagation box 2 via the transfer turntable 14 to continue the propagation operation.

[0056] S3: When a certain number of bacteria are collected in the collection box 42, the container body 1 is removed, the collected bacteria are concentrated, and then the bacteria are dried. After drying, the number of live bacteria and miscellaneous bacteria are tested, and finally the bacteria are sealed and packaged.

[0057] The above are all preferred embodiments of this application, and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. A device for propagating a Rhodopseudomonas palustris agent, characterized by: The utility model relates to a container body (1) is sealed and hollow inside, the container body (1) inside includes the independent expansion tank (2) and sampling tank (4) of each other, the expansion tank (2) is used for bacterial expansion, and the sampling tank (4) is used for collecting bacterial strain after expansion, The utility model relates to a container body (1) is sealed and hollow inside, the container body (1) inside includes the independent expansion tank (2) and sampling tank (4) of each other, the expansion tank (2) is used for bacterial expansion, and the sampling tank (4) is used for collecting bacterial strain after expansion, The utility model relates to a container body (1) is sealed and hollow inside, the container body (1) inside includes the independent expansion tank (2) and sampling tank (4) of each other, the expansion tank (2) is used for bacterial expansion, and the sampling tank (4) is used for collecting bacterial strain after expansion, The utility model relates to a container body (1) is sealed and hollow inside, the container body (1) inside includes the independent expansion tank (2) and sampling tank (4) of each other, the expansion tank (2) is used for bacterial expansion, and the sampling tank (4) is used for collecting bacterial strain after expansion, The utility model relates to a container body (1) is sealed and hollow inside, the container body (1) inside includes the independent expansion tank (2) and sampling tank (4) of each other, the expansion tank (2) is used for bacterial expansion, and the sampling tank (4) is used for collecting bacterial strain after expansion, The utility model relates to a container body (1) is sealed and hollow inside, the container body (1) inside includes the independent expansion tank (2) and sampling tank (4) of each other, the expansion tank (2) is used for bacterial expansion, and the sampling tank (4) is used for collecting bacterial strain after expansion, The utility model relates to a container body (1) is sealed and hollow inside, the container body (1) inside includes the independent expansion tank (2) and sampling tank (4) of each other, the expansion tank (2) is used for bacterial expansion, and the sampling tank (4) is used for collecting bacterial strain after expansion, The utility model relates to a container body (1) is sealed and hollow inside, the container body (1) inside includes the independent expansion tank (2) and sampling tank (4) of each other, the expansion tank (2) is used for bacterial expansion, and the sampling tank (4) is used for collecting bacterial strain after expansion, The utility model relates to a container body (1) is sealed and hollow inside, the container body (1) inside includes the independent expansion tank (2) and sampling tank (4) of each other, the expansion tank (2) is used for bacterial expansion, and the sampling tank (4) is used for collecting bacterial strain after expansion, The utility model relates to a container body (1) is sealed and hollow inside, the container body (1) inside includes the independent expansion tank (2) and sampling tank (4) of each other, the expansion tank (2) is used for bacterial expansion, and the sampling tank (4) is used for collecting bacterial strain after expansion, The utility model relates to a container body (1) is sealed and hollow inside, the container body (1) inside includes the independent expansion tank (2) and sampling tank (4) of each other, the expansion tank (2) is used for bacterial expansion, and the sampling tank (4) is used for collecting bacterial strain after expansion, The utility model relates to a container body (1) is sealed and hollow inside, the container body (1) inside includes the independent expansion tank (2) and sampling tank (4) of each other, the expansion tank (2) is used for bacterial expansion, and the sampling tank (4) is used for collecting bacterial strain after expansion, The utility model relates to a container body (1) is sealed and hollow inside, the container body (1) inside includes the independent expansion tank (2) and sampling tank (4) of each other, the expansion tank (2) is used for bacterial expansion, and the sampling tank (4) is used for collecting bacterial strain after expansion, The utility model relates to a container body (1) is sealed and hollow inside, the container body (1) inside includes the independent expansion tank (2) and sampling tank (4) of each other, the expansion tank (2) is used for bacterial expansion, and the sampling tank (4) is used for collecting bacterial strain after expansion, The utility model relates to a container body (1) is sealed and hollow inside, the container body (1) inside includes the independent expansion tank (2) and sampling tank (4) of each other, the expansion tank (2) is used for bacterial expansion, and the sampling tank (4) is used for collecting bacterial strain after expansion, The utility model relates to a container body (1) is sealed and hollow inside, the container body (1) inside includes the independent expansion tank (2) and sampling tank (4) of each other, the expansion tank (2) is used for bacterial expansion, and the sampling tank (4) is used for collecting bacterial strain after expansion, The utility model relates to a container body (1) is sealed and hollow inside, the container body (1) inside includes the independent expansion tank (2) and sampling tank (4) of each other, the expansion tank (2) is used for bacterial expansion, and the sampling tank (4) is used for collecting bacterial strain after expansion, The utility model relates to a container body (1) is sealed and hollow inside, the container body (1) inside includes the independent expansion tank (2) and sampling tank (4) of each other, the expansion tank (2) is used for bacterial expansion, and the sampling tank (4) is used for collecting bacterial strain after expansion, The utility model relates to a container body (1) is sealed and hollow inside, the container body (1) inside includes the independent expansion tank (2) and sampling tank (4) of each other, the expansion tank (2) is used for bacterial expansion, and the sampling tank (4) is used for collecting bacterial strain after expansion, The utility model relates to a container body (1) is sealed and hollow inside, the container body (1) inside includes the independent expansion tank (2) and sampling tank (4) of each other, the expansion tank (2) is used for bacterial expansion, and the sampling tank (4) is used for collecting bacterial strain after expansion, The utility model relates to a container body (1) is sealed and hollow inside, the container body (1) inside includes the independent expansion tank (2) and sampling tank (4) of each other, the expansion tank (2) is used for bacterial expansion, and the sampling tank (4) is used for collecting bacterial strain after expansion, The utility model relates to a container body (1) is sealed and hollow inside, the container body (1) inside includes the independent expansion tank (2) and sampling tank (4) of each other, the expansion tank (2) is used for bacterial expansion, and the sampling tank (4) is used for collecting bacterial strain after expansion, The utility model relates to a container body (1) is sealed and hollow inside, the container body (1) inside includes the independent expansion tank (2) and sampling tank (4) of each other, the expansion tank (2) is used for bacterial expansion, and the sampling tank (4) is used for collecting bacterial strain after expansion, The utility model relates to a container body (1) is sealed and hollow inside, the container body (1) inside includes the independent expansion tank (2) and sampling tank (4) of each other, the expansion tank (2) is used for bacterial expansion, and the sampling tank (4) is used for collecting bacterial strain after expansion, The utility model relates to a container body (1) is sealed and hollow inside, the container body (1) inside includes the independent expansion tank (2) and sampling tank (4) of each other, the expansion tank (2) is used for bacterial expansion, and the sampling tank (4) is used for collecting bacterial strain after expansion, The utility model relates to a container body (1) is sealed and hollow inside, the container body (1) inside includes the independent expansion tank (2) and sampling tank (4) of each other, the expansion tank (2) is used for bacterial expansion, and the sampling tank (4) is used for collecting bacterial strain after expansion, The utility model relates to a container body (1) is sealed and hollow inside, the container body (1) inside includes the independent expansion tank (2) and sampling tank (4) of each other, the expansion tank (2) is used for bacterial expansion, and the sampling tank (4) is used for collecting bacterial strain after expansion, The utility model relates to a container body (1) is sealed and hollow inside, the container body (1) inside includes the independent expansion tank (2) and sampling tank (4) of each other, the expansion tank (2) is used for bacterial expansion, and the sampling tank (4) is used for collecting bacterial strain after expansion, The utility model relates to a container body (1) is sealed and hollow inside, the container body (1) inside includes the independent expansion tank (2) and sampling tank (4) of each other, the expansion tank (2) is used for bacterial expansion, and the sampling tank (4) is used for collecting bacterial strain after expansion, The utility model relates to a container body (1) is sealed and hollow inside, the Two said positioning rings (52) are respectively provided with magnetic attraction rings (521) on two opposite surfaces, the magnetic attraction rings (521) are coaxially embedded in the positioning rings (52) and are rotationally connected to the positioning rings (52), the two side walls of the scraper (41) near one end of the growth cover (51) are provided with magnetic attraction blocks (414), the magnetic attraction blocks (414) and the magnetic attraction rings (521) are attracted to each other and magnetically connected, and the end of the scraper (41) is in contact with the outer wall of the growth cover (51); The scraper (41) comprises a contact plate (411), a flexible connecting piece (412) and a fixed plate (413), the fixed plate (413) is connected to the collection box (42), the contact plate (411) is located at one end of the fixed plate (413) near the growth cover (51) and is connected to the fixed plate (413) through the flexible connecting piece (412), the magnetic attraction blocks (414) are arranged on the two sides of the contact plate (411), the end of the contact plate (411) is matched with the positioning groove (53) in shape, and the flexible connecting piece (412) can be deformed to allow the contact plate (411) to move to a limit position of the contact plate (411) following the growth cover (51).

2. The equipment for mass propagation of the agent of P. luteola according to claim 1, characterized in that: The conversion turntable (14) is rotationally and sealingly connected with a circulating turntable (16), the circulating turntable (16) is eccentrically arranged in the conversion turntable (14) and has a rotation axis parallel to the axis of the conversion turntable (14), the light emitting members (5) are arranged in multiple and located on the inner side wall of the circulating turntable (16), the multiple light emitting members (5) are parallel to each other and concentrically and spacedly distributed, and the inner side wall of the circulating turntable (16) is further provided with a driving assembly for driving all the light emitting members (5) and the respective corresponding growth covers (51) to simultaneously rotate around the axis of the conversion turntable (14).

3. The equipment for mass propagation of the agent of P. luteola according to claim 1, characterized in that: The light emitting member (5) is a self-luminous member (5) with a power supply.

4. A process for the production of a Rhodopseudomonas palustris agent, characterized by, Preparation of a Rhodopseudomonas palustris agent using the expansion device of the Rhodopseudomonas palustris agent according to any one of claims 1-3, comprising the following steps: S1: first, sufficient liquid medium is added into the expansion box (2), and appropriate enzyme solution is added into the sampling box (4), the pH value and temperature of the medium are adjusted, and then the Rhodopseudomonas palustris to be expanded is inoculated into the expansion box (2), the container body (1) is closed for expansion, and then the growth cover (51) is moved away from the expansion box (2) by the conversion mechanism at a fixed preset time interval, and the strain biofilm on the outer wall of the growth cover (51) is observed, and if the strain biofilm does not meet the expansion requirement, the expansion is continued; S2: if the strain biofilm on the outer wall of the growth cover (51) meets the expansion requirement, the growth cover (51) is moved to the sampling box (4) by the conversion mechanism, the adhesion between the bacteria and the outer wall of the growth cover (51) is weakened by the enzymatic method, and then the biofilm on the outer wall of the growth cover (51) is scraped by the sampling assembly and collected, and then the growth cover (51) is returned to the expansion box (2) by the conversion mechanism. S3: When the bacteria in the sampling assembly are collected to a certain amount, the container body (1) is taken out, the collected bacteria are concentrated, and then dried, and the dried bacteria are detected for viable bacteria and miscellaneous bacteria, and finally sealed and packaged.

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