Application of betaine in increasing rice yield

Abstract

The invention discloses an application of betaine in improving rice yield, according to the application, the betaine is sprayed from an external source to treat rice, the betaine cannot generate other adverse effects on the rice, the growth trend of the rice obviously becomes better, and the ear percentage, the ear length, the hundred-grain weight and the yield are all obviously improved. The method is used for increasing the rice yield, is safe, simple, convenient and efficient, and has a good application prospect.

Description

Technical Field

[0001] The present invention relates to the technical field of rice planting, and more particularly to the application of betaine in improving rice yield. Background Art

[0002] Glycine Betaine, also known as trimethylglycine, is an important quaternary ammonium compound that has been detected in bacteria, cyanobacteria, algae, higher plants and mammals.

[0003] Betaine, a highly effective osmoprotectant, accumulates within cells to combat water loss and ion disturbances caused by environmental stress. It helps maintain osmotic pressure balance inside and outside cells, preventing damage from excessive water loss. Therefore, betaine is considered a major and effective osmoprotectant in plants.

[0004] In addition to osmotic protection, betaine plays an important role in preventing oxidative stress caused by abiotic factors. It can enhance the antioxidant defense mechanism in plants to cope with stress damage. Under stress conditions, betaine can enhance the activity of some antioxidant and detoxification enzymes, thereby enhancing the plant's ability to detoxify ROS generated under stress and increasing its tolerance to stressful environments.

[0005] Therefore, in higher plants, betaine helps to improve tolerance to abiotic stresses (such as salt damage, drought, cold, etc.). For example, betaine seed soaking can increase the content of membrane-associated cold-responsive protein kinase in rapeseed to a certain extent, thereby improving cold resistance, but it has no contribution to yield increase; after spraying betaine under saline-alkali stress, the thousand-grain weight, single-plant grain weight, and yield quality of quinoa were significantly higher than those of the non-spraying treatment group, and the yield reduction caused by saline-alkali stress can be restored; glycine betaine has a mitigating effect on the growth inhibition of eggplant caused by cycloleucine.

[0006] There are also reports that foliar application of betaine can enhance photosynthesis in eggplant seedling leaves, promote plant growth, and improve fruit quality; and that betaine can promote growth and yield in maize varieties by upregulating the oxidative defense system and osmoprotectant accumulation. However, there are no reports on its application in rice. Summary of the Invention

[0007] The purpose of the present invention is to overcome the deficiencies of the prior art and provide an application of betaine in increasing rice yield.

[0008] The first object of the present invention is to provide an application of betaine in increasing rice yield.

[0009] The second object of the present invention is to provide an application of betaine in improving the height of rice plants.

[0010] The third object of the present invention is to provide the use of betaine in improving the ear formation rate of rice.

[0011] The fourth object of the present invention is to provide the use of betaine in increasing the panicle length of rice.

[0012] A fifth object of the present invention is to provide the use of betaine in increasing the 100-grain weight of rice.

[0013] A sixth object of the present invention is to provide a method for increasing rice yield.

[0014] In order to achieve the above object, the present invention is implemented through the following technical solutions:

[0015] The present invention claims protection for the following applications:

[0016] Application of betaine in increasing rice yield.

[0017] Application of betaine in improving the growth of rice plants.

[0018] Application of betaine in improving rice ear formation rate.

[0019] Application of betaine in increasing rice panicle length.

[0020] Application of betaine in increasing 100-grain weight of rice.

[0021] Preferably, the rice is non-stressed rice.

[0022] More preferably, the stress is abiotic stress or biotic stress.

[0023] The present invention also claims a method for increasing rice yield, which comprises exogenously applying betaine to rice.

[0024] Preferably, the method of application used is spraying.

[0025] More preferably, the spraying concentration is 150 mg / L to 200 mg / L, preferably 175 mg / L to 225 mg / L.

[0026] As a specific embodiment, the spraying concentration is 175 mg / L.

[0027] More preferably, the application is carried out during the tillering stage, jointing stage and heading stage.

[0028] As a specific example, the drug is applied once each at the tillering stage, the jointing stage and the heading stage.

[0029] Compared with the prior art, the present invention has the following beneficial effects:

[0030] The present invention utilizes exogenous spraying of betaine to treat rice. Betaine does not have any other adverse effects on the rice, significantly improves the growth trend of the rice, and significantly increases the ear formation rate, ear length, 100-grain weight, and yield. The present invention is safe, simple, efficient, and has great application prospects for increasing rice yield. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 The figure shows the effect of betaine on the panicle length of rice; CK is the control group; GB is the betaine treatment.

[0032] Figure 2 The figure shows the effect of betaine on the 100-grain weight of rice; CK is the control group; GB is the betaine treatment.

[0033] Figure 3 The figure shows the effect of betaine on the ear formation rate of rice; CK is the control group; GB is the betaine treatment. DETAILED DESCRIPTION

[0034] The present invention is further described in detail below with reference to the accompanying drawings and specific examples. The examples are intended only to illustrate the present invention and are not intended to limit the scope of the present invention. The experimental methods used in the following examples are conventional methods unless otherwise specified; the materials and reagents used are commercially available unless otherwise specified.

[0035] Example 1 Betaine treatment of Nipponbare rice

[0036] From May 12, 2024 to July 18, 2024, the Nipponbare variety of rice was planted in Wangzhong Village, Natong Town, Long'an County, Nanning City, Guangxi Zhuang Autonomous Region, and transplanted and cultivated on August 9, 2024.

[0037] The specific processing parties are as follows:

[0038] Rice seedling cultivation: Rice seeds were sterilized with a 3% hydrogen peroxide solution and placed in a petri dish lined with neutral filter paper. An appropriate amount of distilled water was added to accelerate germination. Rice seeds with roughly the same germination degree were evenly distributed on a mesh floating plate and placed in a modified 1 / 2-strength Hoagland rice nutrient solution. The day / night temperature was 30°C / 25°C, the photoperiod was 14 hours, and the light intensity was 200 μmol m -2 s -1 , culture in a constant temperature incubator under a relative humidity of 70% for about five days, select rice seedlings with similar growth trends, and move them to a water basin for root-protected culture.

[0039] Transplanting: The rice seedlings are cultivated for about two months and then transplanted to the field.

[0040] Betaine treatment: Betaine was sprayed once during the tillering, jointing and heading stages of rice. The specific spraying time was August 24, 2024, the second spraying was on September 18, 2024, and the third spraying was on September 27, 2024. Three spraying concentrations were set: 175 (Ⅰ) mg / L, 200 (Ⅱ) mg / L, and 225 (Ⅲ) mg / L. Nipponbare rice without betaine spraying was used as a control.

[0041] Example 2 Effect of betaine on the growth of Nipponbare rice

[0042] 1. Experimental Methods

[0043] The rice treated in Example 1 was photographed on October 25 to record its growth, and the ear formation rate, ear length, 100-grain weight, and yield of the rice were compared before and after the addition of betaine.

[0044] 2. Experimental Results

[0045] result Figures 1 to 3 As shown, exogenous addition of betaine significantly improved rice growth, without any other adverse effects. Spraying different concentrations of betaine improved rice ear formation rate, ear length, 100-grain weight, and yield to varying degrees compared to the blank control. The optimal effect was achieved at a betaine concentration of 175 mg / L.

[0046] Compared with the blank control, the ear formation rate of spraying 175mg / L betaine increased by 3.94%; the ear formation rate of spraying 200mg / L betaine increased by 3.41%; the ear formation rate of spraying 225mg / L betaine increased by 2.76%;

[0047] Compared with the blank control, the ear length increased by 2.74% when sprayed with 175 mg / L betaine, by 2.59% when sprayed with 200 mg / L betaine, and by 2.31% when sprayed with 225 mg / L betaine.

[0048] Compared with the blank control, the 100-grain weight increased by 6.72% after spraying 175 mg / L betaine, 6.14% after spraying 200 mg / L betaine, and 4.13% after spraying 225 mg / L betaine.

[0049] Compared with the blank control, the yield of spraying 175 mg / L betaine increased by 16.34%, the yield of spraying 200 mg / L betaine increased by 12.69%, and the yield of spraying 225 mg / L betaine increased by 9.27%.

Claims

1. Application of betaine in increasing rice yield.

2. Application of betaine in improving the growth rate of rice plants.

3. Application of betaine in improving rice ear formation rate.

4. Application of betaine in increasing rice panicle length.

5. Application of betaine in increasing 100-grain weight of rice.

6. The use according to any one of claims 1 to 5, characterized in that The rice is rice that is not under stress.

7. The use according to claim 6, characterized in that The stress is abiotic stress or biotic stress.

8. A method for increasing rice yield, characterized in that: Betaine was exogenously applied to rice.

9. The method according to claim 8, characterized in that The method of application used was spraying.

10. The method according to claim 9, characterized in that Apply during the tillering, jointing and heading stages.

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