Plum blossom three-resistance composition and application thereof in cosmetics

Abstract

The invention provides a plum blossom three-resistance composition. The plum blossom three-antibody composition comprises a plant extract, nucleic acid and sodium mannose phosphate, wherein the weight ratio of the plant extract to the nucleic acid to the sodium mannose phosphate is (45-50): (45-50): (1-5). According to the composition, brightening is achieved through anti-oxidation and anti-inflammatory / anti-saccharification double channels, the plum blossom extract and ribonucleic acid mainly control and repair pigments, dullness is prevented through micro-ecological management of sodium mannose phosphate, and the composition is suitable for the problems of staying up late muscles, color sinking after inflammation and initial-aging type skin color unevenness.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a plum blossom triple-antibiotic composition and application thereof in cosmetics. Background Art

[0002] As consumers' demand for effective skincare products continues to rise, multi-functional, complex products have become mainstream in the market. Among these, brightening skin tone, anti-oxidation, and anti-aging are core consumer concerns. While traditional whitening or brightening ingredients (such as vitamin C and niacinamide) have some effectiveness, they generally suffer from poor stability, low transdermal absorption, or a single target, resulting in limited or slow brightening effects. Furthermore, dull skin is often associated with multiple mechanisms, including oxidative stress, inflammatory responses, and collagen degradation. Comprehensive improvement cannot be achieved with a single ingredient, thus making the development of a multi-target, synergistic, and complex brightening system crucial.

[0003] In recent years, bispecific antibody (bi-antibody) technology has shown significant advantages in the pharmaceutical field because it can act on two different targets simultaneously, but its application in cosmetics is still in the exploratory stage. Combining bi-antibody technology with skin care ingredients is expected to enhance the brightening effect through a synergistic mechanism, such as simultaneously inhibiting melanin production and promoting keratin metabolism, or combining antioxidant and anti-glycation functions. However, existing bi-antibody active ingredients face many challenges in cosmetic formulations, such as difficulty in preserving their activity, poor compatibility with the matrix, and low transdermal delivery efficiency, which limit their practical application.

[0004] Furthermore, mainstream essence products on the market (such as essence water and essence water) are typically based on aqueous solutions. However, these common formulas struggle to stably carry highly active ingredients and lack penetration-enhancing features, making it difficult for the active ingredients to effectively reach target cells. Therefore, developing a highly effective brightening composition incorporating dual-antibody technology and optimizing its formulation process to match the texture of essence water have become pressing technical challenges in the cosmetics industry. Summary of the Invention

[0005] To address the above issues, the present application provides a plum blossom triple antibody composition. The plum blossom triple antibody composition comprises a plant extract, a nucleic acid, and sodium mannose phosphate, wherein the weight ratio of the plant extract, nucleic acid, and sodium mannose phosphate is 45-50:45-50:1-5. Preferably, the weight ratio of the plant extract, nucleic acid, and sodium mannose phosphate is 47.5-49.5:47.5-49.5:1-5.

[0006] The plant extract comprises at least one of Centella asiatica extract, Calendula officinalis extract, Vitis vinifera extract, Hippophae rhamnoides extract, and Prunus mume extract.

[0007] The active ingredients of the plum blossom extract include flavonoids such as rutin and quercetin, organic acids such as malic acid, citric acid, and succinic acid, phenylethanoid glycosides such as forsythoside, volatile oils, and polysaccharides, which can scavenge free radicals and delay cell aging.

[0008] The nucleic acid is deoxyribonucleic acid and / or ribonucleic acid. The nucleic acid is ribonucleic acid, and the yeast essence N80 is purchased from Ronghai Biological.

[0009] The ribonucleic acid (RNA) originates from yeast cytoplasm and is a small molecule active ingredient obtained by purification after biological enzyme treatment. It is more easily absorbed and utilized by the skin, promotes fibroblast proliferation, effectively promotes collagen synthesis, improves skin elasticity, and reduces wrinkles.

[0010] The deoxyribonucleic acid (DNA) and its derivatives (such as oligonucleotides, DNA sodium, DNA repair enzymes, etc.) have the effects of anti-oxidation, damage repair, anti-aging and moisturizing.

[0011] The sodium mannose phosphate (SODIUM MANNOSE PHOSPHATE, CAS: 70442-25-0) can promote the proliferation of fibroblasts and epidermal keratinocytes, scavenge DPPH free radicals, and promote mitochondrial activity, thereby achieving a significant smoothing and firming effect on the skin, improving the initial signs of visible skin aging, and helping to improve fine lines and wrinkles.

[0012] The plum blossom triple-antibiotic composition can be used in cosmetics, such as essence water, lotion, cream, facial mask, shower gel, etc.

[0013] The second aspect of the present application provides a refined water comprising the plum blossom triple antibody composition, wherein the weight ratio of the plum blossom triple antibody composition to the refined water is 1.8-2.5%; preferably, the weight ratio of the plum blossom triple antibody composition to the refined water is 2.04%.

[0014] The refined water comprises: component A, component B, component C, component D, component E and water;

[0015] Component A includes: glycerin, EDTA-2Na, propylene glycol, xanthan gum, hyaluronic acid, butylene glycol; Component A accounts for 3-5% of the total weight of the essence water; the weight ratio of glycerin, EDTA-2Na, propylene glycol, xanthan gum, hyaluronic acid, butylene glycol is: 1:0.05-0.15:2:0.05:0.01-0.05:1. Preferably, the weight ratio of glycerin, EDTA-2Na, propylene glycol, xanthan gum, hyaluronic acid, butylene glycol is: 1:0.1:2:0.05:0.03:1

[0016] Component B includes butanediol and p-hydroxyacetophenone; component B accounts for 1-2% of the total weight of the refined water; the weight ratio of butanediol to p-hydroxyacetophenone is 1:0.1-1. Preferably, the weight ratio of butanediol to p-hydroxyacetophenone is 1:0.5.

[0017] Component C includes caprylic / capric triglyceride; component C accounts for 0.1-5% of the total weight of the essence water; the caprylic / capric triglyceride can promote the dissolution of rose essential oil, lemongrass essential oil, ascorbyl tetraisopalmitate, etc., while improving the stability of the essence water and the permeability of the niacinamide and plum blossom triple anti-inflammatory composition.

[0018] Component D includes: tocopheryl acetate, cyclosiloxane, stearyl ether, comfrey oil, isohexadecane, ascorbyl tetraisopalmitate, rose essential oil, lemongrass essential oil, and grapefruit essence; component D accounts for 4.5-5.2% of the total weight of the refined water; the weight ratio of the tocopheryl acetate, cyclosiloxane, stearyl ether, comfrey oil, isohexadecane, ascorbyl tetraisopalmitate, rose essential oil, lemongrass essential oil, and grapefruit essence is 0.025:1:0.025:0.3:3.46:0.01:0.0075:0.0075:0.015.

[0019] The cyclosiloxane is Dow's XIAMETER PMX-0345Z Fluid.

[0020] The stearyl ether is ARLAMOL TM PS15E-LQ-(SG)PPG-15 Stearyl Ether.

[0021] The INCI name for this lithospermum oil is Lithospermum Erythrorhizon Root Oil. Its Chinese name is Lithospermum erythrorhizon root oil. It is sourced from the root of Lithospermum erythrorhizon. Its main active ingredients are shikonin, acetylshikonin, β-sitosterol, and polysaccharides.

[0022] Ascorbyl tetraisopalmitate is a vitamin C derivative that can inhibit the production of tyrosinase and melanin in cells and promote collagen production. In terms of molecular weight, it is 6.4 times that of the original vitamin C.

[0023] Rose essential oil is rich in volatile aromatic components, such as citronellol and eucalyptol, which provide multiple skin-care benefits. Its primary effect is deep moisturizing, significantly enhancing the skin's ability to retain moisture.

[0024] The lemongrass essential oil is rich in antioxidant ingredients, which can help remove dead cells on the skin surface, make the skin brighter, and slow down the rate of skin oxidation.

[0025] Component E includes panthenol, Sakura Extract B, plant root extract, ascorbyl glucoside, niacinamide, a plum blossom triple-antibiotic combination, Helichrysum flower water, a preservative, and salt. Component E accounts for 5-6% of the total weight of the refined water. The weight ratio of panthenol, Sakura Extract B, plant root extract, ascorbyl glucoside, niacinamide, a plum blossom triple-antibiotic combination, Helichrysum flower water, a preservative, and salt is 0.2:0.1:1:0.02:0.2:2.04:0.11:0.8:1.

[0026] The Yoshino cherry leaf extract (SAKURA Extract B) can improve skin roughness, inhibit mediators (inhibit arachidonic acid ear edema, inhibit histamine release), and inhibit melanin synthesis (inhibit B16 melanoma cell generation).

[0027] The helichrysanthemum flower water is HELICHRYSUM ITALICUM WATER ORGANIC, which contains triterpenoid compounds, which can enhance the resistance of capillaries and inhibit capillary permeability; at the same time, it can eliminate free radicals and alleviate aging; and it also has a certain inhibitory effect on melanocytes.

[0028] The plant root extract comprises at least one of ginseng root extract, lotus root extract, platycodon root extract, and Codonopsis lantechola root extract. Preferably, the plant root extract comprises ginseng root extract, lotus root extract, platycodon root extract, and Codonopsis lantechola root extract; and the weight ratio of the ginseng root extract, lotus root extract, platycodon root extract, and Codonopsis lantechola root extract is 68:25:2.5-3.5:1-2.

[0029] In the present application, the trade name of the plant root extract is 5ROOT AQUA.

[0030] The preservatives include SpectrastatTM PHL and NEOLONE TM PH 100, Spectrastat TM PHL and NEOLONE TM The weight ratio of PH 100 is 5:3. TM PHL is composed of 1,2-hexanediol, octanoylhydroxamic acid and 1,3-propylene glycol; the NEOLONE TM The INCI code for PH 100 is Phenoxyethanol.

[0031] A third aspect of the present invention provides a method for preparing the purified water, which specifically comprises the following steps:

[0032] S1. Add water and phase A to an emulsifier (homogenize at 3000 rpm for 10 minutes), stir (speed 10-20 rpm) and heat to 80-85 degrees, stirring until completely dissolved.

[0033] S2. Cool down after defoaming. When the temperature drops to 45°C, add the pre-stirred and heated to 60°C dissolved phase B raw materials and stir evenly;

[0034] S3 cools down to 40 degrees, then adds phases C, D, and E into the emulsifying pot and stirs evenly.

[0035] Beneficial effects

[0036] The composition described in this application achieves brightening through three pathways: anti-wrinkle, anti-oxidation, and anti-photodamage. Plum blossom extract and ribonucleic acid focus on pigment regulation and repair, while sodium mannose phosphate microecological management prevents dullness. It is suitable for skin that stays up late, post-inflammatory pigmentation, and uneven skin tone caused by early aging.

[0037] The essence water prepared at the same time has anti-wrinkle and anti-aging effects, and is gentle and non-irritating. The tocopheryl acetate and ascorbyl tetraisopalmitate interact with each other to enhance the antioxidant effect, ensuring that the essence water is refreshing and easy to absorb while maintaining basic moisturizing power. The isohexadecane can enhance the permeability of some oil-soluble active ingredients (such as vitamin E and plant essential oils), and the spreadability of cyclosiloxane helps the active ingredients evenly cover the skin. Implementation Method

[0038] The RNA in the examples was purchased from Ronghai Biological's yeast essence N80.

[0039] The trade name for propylene glycol is Propylene Glycol USP / EP.

[0040] Caprylic / capric triglyceride is sold under the trade name RADIA 7104.

[0041] The trade name of cyclosiloxane is XIAMETER PMX-0345Z Fluid.

[0042] The trade name of PPG-15 stearyl ether is ARLAMOL TM PS15E-LQ-(SG).

[0043] The trade name of Somei Yoshino cherry leaf extract is SAKURA Extract B.

[0044] Example 1

[0045] A plum blossom triple-antibody composition comprises, by weight, 1 part of plum blossom extract, 1 part of ribonucleic acid, and 0.04 part of sodium mannose phosphate.

[0046] Example 2

[0047] A plum blossom triple-antibody composition comprises, by weight, 0.969 parts of plum blossom extract, 0.969 parts of ribonucleic acid, and 0.10 parts of sodium mannose phosphate.

[0048] Example 3

[0049] A plum blossom triple-antibody composition comprises, by weight, 1.01 parts of plum blossom extract, 1.01 parts of ribonucleic acid, and 0.03 parts of sodium mannose phosphate.

[0050] Example 4

[0051] A plum blossom triple-antibody composition comprises, by weight, 0.64 parts of plum blossom extract, 1.4 parts of ribonucleic acid, and 0.01 parts of sodium mannose phosphate.

[0052] Example 5

[0053] A plum blossom triple-antibody composition comprises, by weight, 1.14 parts of plum blossom extract, 0.8 parts of ribonucleic acid, and 0.1 parts of sodium mannose phosphate.

[0054] Example 6

[0055] A kind of refined water containing plum blossom three-antibiotic composition, the formula is as follows

[0056]

[0057]

[0058] The plant root extract includes ginseng root extract, lotus root extract, platycodon root extract, and goat milk root extract; the weight ratio of ginseng root extract, lotus root extract, platycodon root extract, and goat milk root extract is 68:25:3.1:1.9. The plant root extract also includes 1,2-hexanediol as a solvent. The weight ratio of 1,2-hexanediol to goat milk root extract is 2:1.9.

[0059] The preparation method of the refined water specifically comprises the following steps:

[0060] S1. Add water and phase A to an emulsifier (homogenize at 3000 rpm for 10 minutes), stir (speed 10-20 rpm) and heat to 80-85 degrees, stirring until completely dissolved.

[0061] S2. Cool down after defoaming. When the temperature drops to 45°C, add the pre-stirred and heated to 60°C dissolved phase B raw materials and stir evenly;

[0062] S3 cools down to 40 degrees, then adds phases C, D, and E into the emulsifying pot and stirs evenly.

[0063] Example 7

[0064] A kind of refined water containing plum blossom three-antibiotic composition, the formula is as follows

[0065]

[0066]

[0067] The specific implementation of the plant root extract is the same as that of Example 6.

[0068] Example 8

[0069] A kind of refined water containing plum blossom three-antibiotic composition, the formula is as follows

[0070]

[0071] The specific implementation of the plant root extract is the same as that of Example 6.

[0072] Example 9

[0073] A kind of refined water containing plum blossom three-antibiotic composition, the formula is as follows

[0074]

[0075] The specific implementation of the plant root extract is the same as that of Example 6.

[0076] Performance testing and data

[0077] (1) Anti-aging research

[0078] The composition of Examples 1-5 was diluted with deionized water and added to a culture medium of mouse embryonic fibroblasts (NIH3T3), with deionized water as a blank control. The final test concentration was 0.1 mg / mL.

[0079] After culturing for 48 hours, the cells were stained with the MTT method and the absorbance at 550 nm was measured using a microplate reader. The proliferation rate of the blank control was 100%, and the blank control was used as a reference to evaluate the proliferation effect on mouse embryonic fibroblasts.

[0080] The collagen production was measured using a type I collagen assay kit. The blank control had a production rate of 100%. The blank control was used as a reference to evaluate the effect of promoting type I collagen synthesis. The results are shown in the following table.

[0081]

[0082]

[0083] (2) DPPH free radical scavenging rate

[0084] Prepare blank with DPPH solution and blank without DPPH solution; sample with DPPH solution and sample without DPPH solution; the sample refers to the composition of Examples 1-5. The sample with DPPH solution is 0.2 mL of sample and 4 mL of 0.1 mmol / L DPPH solution.

[0085] The reaction was carried out in the dark at room temperature for 20 min, and the absorbance OD value was read at 517 nm.

[0086] The calculation formula of DPPH free radical scavenging rate is as follows:

[0087] Clearance rate (%) = [(blank with DPPH solution OD - blank without DPPH solution OD) - (sample with DPPH solution OD - sample without DPPH solution OD)] / (blank with DPPH solution OD - blank without DPPH solution OD).

[0088] Composition Free radical scavenging rate (%) Example 1 96 Example 2 92 Example 3 98 Example 4 76 Example 5 89

[0089] (3) Human anti-wrinkle efficacy evaluation test

[0090] Take an appropriate amount of the essence water in Example 6, apply it evenly to the skin around the eyes, and gently massage along the skin texture around the eyes until it is absorbed.

[0091] Frequency of use: once a day in the morning and evening.

[0092] The number of subjects was 30 (6 males and 24 females); age range: 30-60 years old.

[0093] Test area: corner of eye.

[0094] Testing period: 28 days.

[0095] Photos were taken before and after using the samples, and the skin elasticity tester was used for testing.

[0096] Average depth test of crow's feet: average improvement rate = (average value before use - average value after use) / (average value before use) * 100%.

[0097] Crow's feet wrinkle number test: average improvement rate = (average value before use - average value after use) / (average value before use) * 100%.

[0098] Crow's feet volume test: average improvement rate = (average value before use - average value after use) / (average value before use) * 100%.

[0099] Crow's feet length test: average improvement rate = (average value before use - average value after use) / (average value before use) * 100%.

[0100] Test results table

[0101]

[0102] (4) Comprehensive evaluation experiment on human trials: The test subjects are the same as those in the human anti-wrinkle efficacy evaluation test.

[0103] User experience rating table

[0104] Serial number question Score 1 The sample is gentle and non-irritating when used; □5□4□3□2□1 2 The sample has a refreshing texture and no sticky feeling; □5□4□3□2□1 3 No skin tightness after using this sample □5□4□3□2□1 4 Overall, I am satisfied with this sample. □5□4□3□2□1

[0105] 5 points means completely agree, 4 points means relatively agree, 3 points means both agree and disagree, 2 points means relatively disagree, and 1 point means completely disagree. There are 30 people in total, and the total scores are recorded in the table below.

[0106] Example Question 1 Question 2 Question 3 Question 4 Example 6 150 150 150 150 Example 7 125 150 130 131 Example 8 149 128 150 138 Example 9 128 129 147 129

[0107] (5) Stability test

[0108] The skin care products prepared in Examples 6-9 were placed at 0°C, 25°C, and 40°C for 90 days, and the product properties and color were checked. The test results are shown in the table below.

[0109] Example 0℃ 25℃ 40℃ Example 6 No change No change No change Example 7 Layering Layering Layering Example 8 Layering Layering Layering Example 9 Layering Layering Layering

[0110] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the above embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A plum blossom triple-antibody composition, characterized in that: The plum blossom triple antibody composition comprises plant extract, nucleic acid and sodium mannose phosphate, and the weight ratio of the plant extract, nucleic acid and sodium mannose phosphate is: 45-50:45-50:1-5.

2. The plum blossom triple-antibody composition according to claim 1, characterized in that: The plant extract comprises at least one of Centella asiatica extract, Calendula officinalis extract, Vitis vinifera extract, Hippophae rhamnoides extract, and Prunus mume extract.

3. The plum blossom triple-antibody composition according to claim 1, wherein: The nucleic acid is deoxyribonucleic acid and / or ribonucleic acid.

4. The plum blossom triple-antibody composition according to any one of claims 1 to 3, characterized in that: The weight ratio of the plant extract, nucleic acid and sodium mannose phosphate is: 47.5-49.5:47.5-49.5:1-5.

5. A cosmetic comprising the plum blossom triple-antibiotic composition according to any one of claims 1 to 4, characterized in that: The weight ratio of the plum blossom triple-antibiotic composition to the cosmetics is 1.8-2.5%.

6. The cosmetic according to claim 5, wherein: The cosmetics include essence water, lotion, cream, facial mask, and shower gel.

7. The cosmetic according to claim 5, wherein: The cosmetic comprises: component A, component B, component C, component D, component E and water; Component A includes: glycerin, EDTA-2Na, propylene glycol, xanthan gum, hyaluronic acid, butylene glycol; Component B includes: butanediol, p-hydroxyacetophenone; Component C includes: caprylic / capric triglyceride; Component D includes: tocopheryl acetate, cyclosiloxane, stearyl ether, comfrey oil, isohexadecane, ascorbyl tetraisopalmitate, rose essential oil, lemongrass essential oil, grapefruit essence.

8. The cosmetic according to claim 6, wherein: Also includes Yoshino cherry leaf extract, ascorbyl glucoside, niacinamide, Helichrysum flower water, preservatives, and salt.

9. The cosmetic according to claim 5, wherein: Component A accounts for 3-5% of the total weight of the cosmetics; component B accounts for 1-2% of the total weight of the essence water; component C accounts for 0.1-5% of the total weight of the essence water; and component D accounts for 4.5-5.2% of the total weight of the essence water.

10. The cosmetic according to claim 5, wherein: The preservatives include Spectrastat TM PHL and NEOLONE TM PH 100.

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