A clover three-antigen composition and its use in cosmetics

Abstract

The application provides a plum blossom three-antibody composition. The plum blossom three-antibody composition comprises a plant extract, nucleic acid and sodium mannose phosphate, and the weight ratio of the plant extract, nucleic acid and sodium mannose phosphate is 45-50:45-50:1-5. The composition described in the application realizes lightening through the antioxidant + anti-inflammatory / anti-glycation double channels. The plum blossom extract and ribonucleic acid mainly attack pigment regulation and repair, and sodium mannose phosphate microecological management prevents dullness, and is suitable for the problems of night-owl skin, post-inflammatory hyperpigmentation and uneven skin tone of the primary aging type.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, specifically to a plum blossom three-anti-inflammatory composition and its application in cosmetics. Background Technology

[0002] As consumers' demands for skincare product efficacy continue to rise, multi-functional composite products have become the mainstream in the market. Among these, brightening skin tone, anti-oxidation, and anti-aging are core consumer concerns. While traditional whitening or brightening ingredients (such as vitamin C and niacinamide) have some effect, they generally suffer from poor stability, low transdermal absorption, or single-target effects, resulting in limited or slow-acting brightening effects. Furthermore, dull skin is often related to multiple mechanisms such as oxidative stress, inflammatory responses, and collagen degradation, making it difficult for a single ingredient to achieve comprehensive improvement. Therefore, developing a multi-target synergistic brightening system is of great significance.

[0003] In recent years, bispecific antibody (Biantibody) technology has shown significant advantages in the pharmaceutical field due to its ability to act on two different targets simultaneously, but its application in cosmetics is still in the exploratory stage. Combining Biantibody technology with skincare ingredients is expected to enhance brightening effects through synergistic mechanisms, such as simultaneously inhibiting melanin production and promoting keratin metabolism, or possessing both antioxidant and anti-glycation functions. However, existing Biantibody active ingredients face many challenges in cosmetic formulations, such as difficulty in preserving activity, poor compatibility with the matrix, and low transdermal delivery efficiency, which limits their practical application.

[0004] Furthermore, mainstream serum products on the market (such as essence waters and essence serums) are usually water-based, but ordinary formulas are difficult to stably carry highly active ingredients and lack penetration-enhancing designs, making it difficult for the active ingredients to effectively act on target cells. Therefore, developing a highly effective brightening composition that incorporates dual-anti-inflammatory technology and optimizing its formulation process to suit the texture of essence waters has become a pressing technical challenge in the cosmetics industry. Summary of the Invention

[0005] To address the aforementioned problems, this application provides a plum blossom three-antibiotic composition. The plum blossom three-antibiotic composition comprises plant extracts, nucleic acids, and sodium mannose phosphate, wherein the weight ratio of the plant extracts, nucleic acids, and sodium mannose phosphate is 45-50:45-50:1-5. Preferably, the weight ratio of the plant extracts, nucleic acids, and sodium mannose phosphate is 47.5-49.5:47.5-49.5:1-5.

[0006] The plant extracts include at least one of Centella asiatica extract, Calendula officinalis extract, Vitis vinifera extract, Hippophae rhamnoides extract, and Prunus mume extract.

[0007] The active ingredients in the plum blossom extract include rutin, quercetin, flavonoids, organic acids such as malic acid, citric acid, and succinic acid, phenylethyl glycosides such as forsythoside, volatile oils, and polysaccharides. These components can scavenge free radicals and delay cell aging.

[0008] The nucleic acid is deoxyribonucleic acid and / or ribonucleic acid. The nucleic acid is ribonucleic acid, and the purchased product is Ronghai Biotechnology's Yeast Extract N80.

[0009] The ribonucleic acid (RNA) is derived from yeast cytoplasm and is a small molecule active ingredient refined through biological enzyme treatment. It is more easily absorbed and utilized by the skin, promotes fibroblast proliferation, effectively promotes collagen synthesis, improves skin elasticity, and reduces wrinkles.

[0010] The deoxyribonucleic acid (DNA) and its derivatives (such as oligonucleotides, sodium DNA, DNA repair enzymes, etc.) have antioxidant, damage repair, anti-aging and moisturizing effects.

[0011] The sodium mannose phosphate (CAS: 70442-25-0) can promote the proliferation of fibroblasts and epidermal keratinocytes, scavenge DPPH free radicals, and promote mitochondrial activity, thereby achieving a significant skin-smoothing and firming effect, improving the initial signs of visible skin aging, and helping to improve fine lines and wrinkles.

[0012] The plum blossom three-antibody composition can be used in cosmetics, such as essence water, lotion, face cream, face mask, shower gel, etc.

[0013] A second aspect of this application provides purified water comprising the aforementioned three-antibiotic composition, wherein the three-antibiotic composition accounts for 1.8-2.5% of the purified water by weight; preferably, the three-antibiotic composition accounts for 2.04% of the purified water by weight.

[0014] The refined water comprises: component A, component B, component C, component D, component E, and water;

[0015] Component A includes: glycerin, EDTA-2Na, propylene glycol, xanthan gum, hyaluronic acid, butylene glycol; Component A accounts for 3-5% of the total weight of the essence water; the weight ratio of glycerin, EDTA-2Na, propylene glycol, xanthan gum, hyaluronic acid, butylene glycol is: 1: 0.05-0.15: 2: 0.05: 0.01-0.05: 1. Preferably, the weight ratio of glycerin, EDTA-2Na, propylene glycol, xanthan gum, hyaluronic acid, butylene glycol is: 1: 0.1: 2: 0.05: 0.03: 1

[0016] Component B includes: butylene glycol, p-hydroxyacetophenone; Component B accounts for 1-2% of the total weight of the essence water; the weight ratio of the butylene glycol and p-hydroxyacetophenone is 1: 0.1-1. Preferably, the weight ratio of the butylene glycol and p-hydroxyacetophenone is 1: 0.5.

[0017] Component C includes: triglyceride caprylate / caprate; Component C accounts for 0.1-5% of the total weight of the essence water; the triglyceride caprylate / caprate can promote the dissolution of rose essential oil, lemongrass essential oil, tetraisopalmitate ascorbate, etc., and at the same time improve the stability of the essence water and the permeability of niacinamide and the plum blossom triple anti-composition.

[0018] Component D includes: tocopheryl acetate, cyclomethicone, stearyl ether, arnebia oil, isododecane, tetraisopalmitate ascorbate, rose essential oil, lemongrass essential oil, grapefruit essence; Component D accounts for 4.5-5.2% of the total weight of the essence water; the weight ratio of tocopheryl acetate, cyclomethicone, stearyl ether, arnebia oil, isododecane, tetraisopalmitate ascorbate, rose essential oil, lemongrass essential oil, grapefruit essence is 0.025: 1: 0.025: 0.3: 3.46: 0.01: 0.0075: 0.0075: 0.015.

[0019] The cyclomethicone is Dow Corning XIAMETER PMX-0345Z Fluid.

[0020] The stearyl ether is Croda ARLAMOL TM PS15E-LQ-(SG)PPG-15 stearyl ether.

[0021] The INCI name of the arnebia oil: Lithospermum Erythrorhizon Root Oil. Chinese name: Purple Root Oil, Arnebia Oil. Source: Extracted from the roots of Lithospermum erythrorhizon. Main active ingredients: Shikonin, Acetylshikonin, β-Sitosterol, Polysaccharides.

[0022] The ascorbate tetraisopalmitate is a vitamin C derivative that can inhibit the production of intracellular tyrosinase and melanin, and promote collagen production. In terms of molecular weight, it is 6.4 times that of the original vitamin C.

[0023] The rose essential oil contains abundant volatile aromatic components, such as citronellol and eucalyptol, providing multiple nourishing benefits to the skin. Its primary function is deep moisturizing, significantly enhancing the skin's ability to retain moisture.

[0024] The lemongrass essential oil contains rich antioxidants, which can help remove dead skin cells from the skin's surface, making the skin brighter and slowing down the rate of skin oxidation.

[0025] Component E includes: panthenol, Somei Yoshino cherry leaf extract, plant root extract, ascorbyl glucoside, nicotinamide, plum blossom three-antibiotic composition, immortelle flower water, preservative, and salt. Component E accounts for 5-6% of the total weight of the purified water. The weight ratio of panthenol, Somei Yoshino cherry leaf extract (SAKURA Extract B), plant root extract, ascorbyl glucoside, nicotinamide, plum blossom three-antibiotic composition, immortelle flower water, preservative, and salt is 0.2:0.1:1:0.02:0.2:2.04:0.11:0.8:1.

[0026] The Somei Yoshino cherry leaf extract (SAKURA Extract B) can improve rough skin, inhibit mediators (inhibit arachidonic acid ear edema, inhibit histamine free), and inhibit melanin synthesis (inhibit the generation of B16 melanoma cells).

[0027] The herb water is HELICHRYSUM ITALICUM WATER ORGANIC, which contains triterpenoids that enhance capillary resistance and inhibit capillary permeability; it also eliminates free radicals and slows down aging; and it has a certain inhibitory effect on melanocytes.

[0028] The plant root extract includes at least one of ginseng root extract, lotus root extract, platycodon root extract, and goat's milk (codonopsis lanCEOLA TA) root extract. Preferably, the plant root extract includes ginseng root extract, lotus root extract, platycodon root extract, and goat's milk root extract; the weight ratio of ginseng root extract, lotus root extract, platycodon root extract, and goat's milk root extract is 68:25:2.5-3.5:1-2.

[0029] In this application, the plant root extract is marketed under the name 5ROOT AQUA.

[0030] The preservative includes Spectrastat.TM PHL and NEOLONE TM PH 100, Spectrastat TM PHL and NEOLONE TM The weight ratio of PH 100 is 5:3. The Spectrastat... TM PHL is composed of 1,2-hexanediol, octanoyl hydroxamic acid, and 1,3-propanediol; NEOLONE TM The INCI of PH 100 is Phenoxyethanol.

[0031] A third aspect of the present invention provides a method for preparing the purified water, specifically comprising the following:

[0032] S1. Add water and phase A to the emulsification pot (homogenize at 3000 rpm for 10 minutes), stir (10-20 rpm) and heat to 80-85 degrees Celsius, stirring until completely dissolved.

[0033] S2. After defoaming, cool down to 45°C, add the B-phase raw material that has been pre-dissolved by stirring and heated to 60°C, and stir evenly.

[0034] S3 is cooled to 40 degrees Celsius, and then phases C, D, and E are added to the emulsifying pot and stirred evenly.

[0035] Beneficial effects

[0036] The composition described in this application achieves brightening through three pathways: anti-wrinkle, anti-oxidation, and anti-photodamage. Plum blossom extract and ribonucleic acid mainly target pigment regulation and repair, while sodium mannose phosphate manages the microecological environment to prevent dullness. It is suitable for skin that has been exposed to late nights, post-inflammatory hyperpigmentation, and uneven skin tone in the early signs of aging.

[0037] Meanwhile, the prepared essence water has anti-wrinkle and anti-aging effects, and is gentle and non-irritating. The tocopheryl acetate and ascorbate tetraisopalmitate interact to enhance the antioxidant effect. This ensures that the essence water is refreshing and easy to absorb, while maintaining basic moisturizing power. Isohexadecane can enhance the permeability of some oil-soluble active ingredients (such as vitamin E and plant essential oils), while the spreadability of cyclosiloxane helps the active ingredients to evenly cover the skin. Implementation

[0038] In this example, the ribonucleic acid was purchased from Ronghai Biotechnology's yeast extract N80.

[0039] The trade name for propylene glycol is Propylene Glycol USP / EP.

[0040] The trade name for caprylic / capric triglyceride is RADIA 7104.

[0041] The trade name for the cyclosiloxane is XIAMETERPMX-0345Z Fluid.

[0042] PPG-15 stearyl ether is marketed as ARLAMOL. TM PS15E-LQ-(SG).

[0043] The product name for the Yoshino cherry leaf extract is SAKURA Extract B.

[0044] Example 1

[0045] A plum blossom three-antibiotic composition, by weight, comprises: 1 part plum blossom extract; 1 part ribonucleic acid; and 0.04 parts sodium mannose phosphate.

[0046] Example 2

[0047] A plum blossom three-antibiotic composition, by weight, comprises: 0.969 parts of plum blossom extract; 0.969 parts of ribonucleic acid; and 0.10 parts of sodium mannose phosphate.

[0048] Example 3

[0049] A plum blossom three-antibiotic composition, by weight, comprises: 1.01 parts plum blossom extract; 1.01 parts ribonucleic acid; and 0.03 parts sodium mannose phosphate.

[0050] Example 4

[0051] A plum blossom three-antibiotic composition, by weight, comprises: 0.64 parts plum blossom extract; 1.4 parts ribonucleic acid; and 0.01 parts sodium mannose phosphate.

[0052] Example 5

[0053] A plum blossom three-antibiotic composition, by weight, comprises: 1.14 parts plum blossom extract; 0.8 parts ribonucleic acid; and 0.1 parts sodium mannose phosphate.

[0054] Example 6

[0055] A concentrated water containing a plum blossom three-antibiotic composition, the formula is as follows:

[0056]

[0057]

[0058] The plant root extracts include ginseng root extract, lotus root extract, platycodon root extract, and goat's milk root extract; the weight ratio of ginseng root extract, lotus root extract, platycodon root extract, and goat's milk root extract is 68:25:3.1:1.9. The plant root extracts also include the solvent 1,2-hexanediol. The weight ratio of 1,2-hexanediol to goat's milk root extract is 2:1.9.

[0059] The method for preparing the purified water specifically includes the following:

[0060] S1. Add water and phase A to the emulsification pot (homogenize at 3000 rpm for 10 minutes), stir (10-20 rpm) and heat to 80-85 degrees Celsius, stirring until completely dissolved.

[0061] S2. After defoaming, cool down to 45°C, add the B-phase raw material that has been pre-dissolved by stirring and heated to 60°C, and stir evenly.

[0062] S3 is cooled to 40 degrees Celsius, and then phases C, D, and E are added to the emulsifying pot and stirred evenly.

[0063] Example 7

[0064] A concentrated water containing a plum blossom three-antibiotic composition, the formula is as follows:

[0065]

[0066]

[0067] The specific implementation method of the plant root extract is the same as in Example 6.

[0068] Example 8

[0069] A concentrated water containing a plum blossom three-antibiotic composition, the formula is as follows:

[0070]

[0071] The specific implementation method of the plant root extract is the same as in Example 6.

[0072] Example 9

[0073] A concentrated water containing a plum blossom three-antibiotic composition, the formula is as follows:

[0074]

[0075] The specific implementation method of the plant root extract is the same as in Example 6.

[0076] Performance testing and data

[0077] (1) Anti-aging research

[0078] The compositions of Examples 1-5 were diluted with deionized water and added to mouse embryonic fibroblast (NIH3T3) culture medium, with deionized water as a blank control, and the final concentration was tested to be 0.1 mg / mL.

[0079] After 48 hours of culture, the cells were stained with MTT assay and the absorbance at 550 nm was measured using an enzyme-linked immunosorbent assay (ELISA) reader. The proliferation rate of the blank control was 100%. The effect on the proliferation of mouse embryonic fibroblasts was evaluated by comparing the blank control with the blank control.

[0080] Collagen production was measured using a type I collagen assay kit. The production rate of the blank control was 100%, and the effect of promoting type I collagen synthesis was evaluated by comparing it with the blank control. The results are shown in the table below.

[0081]

[0082]

[0083] (2) DPPH free radical scavenging rate

[0084] The blank was prepared with and without DPPH solution; the sample was prepared with and without DPPH solution; the sample refers to the compositions of Examples 1-5. The sample with DPPH solution consisted of 0.2 mL of sample and 4 mL of 0.1 mmol / L DPPH solution.

[0085] The absorbance OD value was read at 517 nm after reacting at room temperature in the dark for 20 min.

[0086] The formula for calculating DPPH free radical scavenging rate is as follows:

[0087] Clearance rate (%) = [(OD of blank with DPPH solution - OD of blank without DPPH solution) - (OD of sample with DPPH solution - OD of sample without DPPH solution)] / (OD of blank with DPPH solution - OD of blank without DPPH solution).

[0088] Composition Free radical scavenging rate (%) Example 1 96 Example 2 92 Example 3 98 Example 4 76 Example 5 89

[0089] (3) Evaluation test of anti-wrinkle efficacy in humans

[0090] Take an appropriate amount of the essence water from Example 6 and apply it evenly to the skin around the eyes. Gently massage along the skin texture around the eyes until absorbed.

[0091] Frequency of use: Once in the morning and once in the evening.

[0092] The number of participants was 30 (6 males and 24 females); age range: 30-60 years.

[0093] Test area: corner of the eye.

[0094] Test period: 28 days.

[0095] Photos were taken before and after the sample was used, and the results were tested using a skin elasticity tester.

[0096] Average depth test of crow's feet wrinkles: Average improvement rate = (mean before use - mean after use) / (mean before use) * 100%.

[0097] Crow's feet wrinkle count test: Average improvement rate = (mean before use - mean after use) / (mean before use) * 100%.

[0098] Crow's feet volume test: Average improvement rate = (mean value before use - mean value after use) / (mean value before use) * 100%.

[0099] Crow's feet length test: Average improvement rate = (mean before use - mean after use) / (mean before use) * 100%.

[0100] Test Results Table

[0101]

[0102] (4) Comprehensive evaluation experiment on human trial: The subjects selected for this test are the same as those selected for the human anti-wrinkle efficacy evaluation experiment.

[0103] User Experience Rating Scale

[0104] Serial Number question Score 1 This sample is gentle and non-irritating. □5□4□3□2□1 2 The sample has a refreshing, non-sticky texture. □5□4□3□2□1 3 No tightness felt on the skin after using the sample. □5□4□3□2□1 4 Overall, I am satisfied with the sample. □5□4□3□2□1

[0105] 5 points indicates complete agreement, 4 points indicates partial agreement, 3 points indicates both agreement and disagreement, 2 points indicates partial disagreement, and 1 point indicates complete disagreement. There are 30 people in total. The total scores are recorded in the table below.

[0106] Example Question 1 Question 2 Question 3 Question 4 Example 6 150 150 150 150 Example 7 125 150 130 131 Example 8 149 128 150 138 Example 9 128 129 147 129

[0107] (5) Stability test

[0108] The skincare products prepared in Examples 6-9 were placed at 0℃, 25℃, and 40℃ for 90 days, and the product properties and color were observed. The test results are shown in the table below.

[0109] Example 0℃ 25℃ 40℃ Example 6 No change No change No change Example 7 Layering Layering Layering Example 8 Layering Layering Layering Example 9 Layering Layering Layering

[0110] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A refined water containing a plum blossom three-antibiotic composition, characterized in that, Calculated by weight, including: Component A: 1,000 parts glycerin EDTA-2Na 0.100 parts 2,000 parts of propylene glycol 0.050 parts of xanthan gum Hyaluronic acid 0.030 parts 1,000 parts of butylene glycol Component B: 1,000 parts of butylene glycol 0.500 parts of p-hydroxyacetophenone Component C: Caprylic / capric triglyceride 0.250 parts Component D: 0.025 parts of tocopherol acetate 1,000 parts of cyclosiloxane 0.025 parts of PPG-15 stearyl ether Comfrey oil 0.300 parts 3.460 parts of isohexadecane 0.010 parts of ascorbic acid tetraisopalmitate 0.0075 parts rose essential oil Lemongrass essential oil 0.0075 parts Grapefruit flavoring 0.015 parts Component E: Panthenol 0.200 parts 0.100 parts of Yoshino cherry leaf extract 1,000 parts of plant root extract Ascorbate glucoside 0.020 parts Nicotinamide 0.200 parts 1,000 parts of plum blossom extract 1,000 ribonucleic acid samples Sodium mannose phosphate 0.040 parts 0.110 parts of helichrysanthemum flower water Spectrastat™ PHL 0.500 copies NEOLONE™ PH 100 0.300 servings 1,000 parts salt Add water to bring the total to 100 servings.

2. The purified water containing the plum blossom three-antibiotic composition according to claim 1, characterized in that, The plant root extracts include ginseng root extract, lotus root extract, platycodon root extract and goat milk root extract; the weight ratio of ginseng root extract, lotus root extract, platycodon root extract and goat milk root extract is 68:25:3.1:1.

9.

3. The method for preparing purified water according to claim 1 or 2, characterized in that, Including the following: S1. Add water and phase A to the emulsifying pot, turn on the homogenizer to 3000 rpm, homogenize for 10 minutes, stir at 10-20 rpm and heat to 80-85 degrees, stirring until completely dissolved; S2. After defoaming, cool down to 45°C, add the B-phase raw material that has been pre-dissolved by stirring and heated to 60°C, and stir evenly. S3 is cooled to 40 degrees Celsius, and then phases C, D, and E are added to the emulsifying pot and stirred evenly.

Images