An oral liquid containing collagen peptides, its preparation method and application

Abstract

This invention belongs to the field of oral liquid technology, specifically relating to an oral liquid containing collagen peptides, its preparation method, and its application. The oral liquid comprises the following components by weight percentage: 10-30% collagen peptides, 0.5-1.2% astaxanthin-ascorbic acid complex, 0.05-0.3% sodium hyaluronate, 0.1-0.5% γ-aminobutyric acid, 3-8% erythritol, 0.05-1% stabilizer, 0.1-6.0% concentrated fruit juice or fruit powder, 0.05-2.0% food flavoring, and the balance being water; the structural formula of the astaxanthin-ascorbic acid complex is: [Insert structural formula here]. This invention chemically combines astaxanthin and ascorbic acid, which not only improves the water solubility of astaxanthin but also enhances the stability of ascorbic acid. Combined with collagen peptides, this results in an oral liquid with good stability and flavor, and long-term use can improve facial skin elasticity.

Description

Technical Field

[0001] This invention belongs to the field of oral liquid technology, specifically relating to an oral liquid containing collagen peptides, its preparation method, and its application. Background Technology

[0002] As we age, environmental pollution, and radiation from electronic devices exacerbate skin health issues, especially on the face. During facial aging, collagen and elastin fibers are gradually lost, leading to wrinkles, fine lines, and sagging skin. Furthermore, the accumulation of free radicals is a significant contributing factor to skin aging; therefore, antioxidants are crucial in combating skin aging.

[0003] Currently, collagen supplementation is a common method to improve skin condition. However, traditional collagen has a large molecular weight, making it difficult for the body to fully absorb it. Collagen peptides, as hydrolyzed products of collagen, have a small molecular weight and high bioactivity, allowing them to be absorbed more effectively by the body and participate in the synthesis and repair of skin collagen.

[0004] Antioxidants also play a crucial role in improving facial skin condition. Astaxanthin, as a natural antioxidant, possesses extremely strong antioxidant capabilities, effectively removing harmful substances such as nitrogen dioxide, sulfides, and disulfides, reducing lipid peroxidation, minimizing free radical damage to facial skin cells, delaying skin aging, and improving dryness and roughness. However, its water-insoluble nature limits its application in water-soluble systems, such as water-soluble facial care products and cosmetics. Ascorbic acid (vitamin C) is also an important antioxidant. As a water-soluble vitamin, it not only participates in various biochemical reactions in the body, protecting facial skin cells from oxidative damage, but also promotes collagen synthesis, maintaining skin elasticity and radiance. However, ascorbic acid aqueous solutions are sensitive to light, heat, and oxygen, and are easily decomposed, affecting its effectiveness in improving facial skin condition.

[0005] While some research has focused on improving the water solubility of astaxanthin or enhancing the stability of ascorbic acid, these methods often have limitations. For example, physical methods such as microencapsulation and nano-sizing can improve the water solubility of astaxanthin to some extent, but may affect its antioxidant activity. On the other hand, methods to improve the stability of ascorbic acid, such as adding stabilizers, may introduce other impurities or affect the product's taste and safety. Furthermore, single-ingredient products often fail to meet the increasingly diverse skincare needs of consumers. For instance, simple antioxidants may not be sufficient to address issues such as dry skin and lack of elasticity, requiring the combined action of other ingredients.

[0006] In view of the above problems, the present invention chemically combines astaxanthin and ascorbic acid and introduces collagen peptides to develop an oral liquid containing collagen peptides. Summary of the Invention

[0007] In order to overcome the shortcomings of the prior art, the primary objective of this invention is to provide an oral liquid containing collagen peptides, which has good stability, taste and flavor, and can improve facial skin elasticity and condition with long-term use.

[0008] Another objective of this invention is to provide a simple method for preparing the oral liquid containing collagen peptides described above.

[0009] A third objective of this invention is to provide an application of the above-mentioned oral liquid containing collagen peptides in the preparation of anti-aging products.

[0010] The above objectives of this invention are achieved through the following technical solutions:

[0011] An oral liquid containing collagen peptides comprises the following ingredients by weight percentage: 10-30% collagen peptides, 0.5-1.2% astaxanthin-ascorbic acid complex, 0.05-0.3% sodium hyaluronate, 0.1-0.3% γ-aminobutyric acid, 3-8% erythritol, 0.05-1.0% stabilizer, 0.1-6.0% concentrated fruit juice or fruit powder, 0.05-2.0% food flavoring, and the balance being water; the structural formula of the astaxanthin-ascorbic acid complex is as follows:

[0012] .

[0013] Furthermore, the preparation process of the astaxanthin-ascorbic acid complex is as follows:

[0014] (1) Astaxanthin and succinic anhydride were added to dioxane, and then triethylamine was added to react. After the reaction was completed, the reaction solution was purified to obtain intermediate 1. The structural formula of intermediate 1 is as follows:

[0015] ;

[0016] (2) Intermediate 1, 4-formylphenylboronic acid and triethylamine were added to acetic anhydride and reacted under heating conditions. The reaction solution was post-treated to obtain intermediate 2. The structural formula of intermediate 2 is:

[0017] ;

[0018] (3) Add intermediate 2 to a 0.1-0.2 mol / L sodium bicarbonate solution, then add ascorbic acid and stir to react. After the reaction is completed, the reaction solution is treated to obtain the astaxanthin-ascorbic acid complex.

[0019] Further, in step (1), the molar ratio of astaxanthin, succinic anhydride and triethylamine is 1:(5-10):(2-4); the reaction temperature is 50-60℃ and the time is 15-20 h.

[0020] Further, in step (2), the molar ratio of intermediate 1,4-formylphenylboronic acid and triethylamine is 1:(2-3):(2-3); the heating temperature is 85-95℃; and the reaction time is 5-7 h.

[0021] Further, in step (3), the ratio of intermediate 2, ascorbic acid and sodium bicarbonate solution is 1 mmol: (3-4) mmol: (10-12) mL; the reaction time is 18-24 h.

[0022] Furthermore, the collagen peptide is a fish collagen tripeptide; the molecular weight of the collagen peptide is 500-1000 Da; the molecular weight of the sodium hyaluronate is 8-40 wDa; and the stabilizer is selected from at least one of xanthan gum, sodium carboxymethyl cellulose, pectin, gum arabic, locust bean gum, and konjac gum.

[0023] Furthermore, the concentrated fruit juice or fruit powder can be any conventional product, determined after evaluation based on market preferences. More preferably, the concentrated fruit juice or fruit powder is selected from at least one of the following: concentrated pomegranate juice, concentrated blueberry juice, concentrated passion fruit juice, concentrated grape juice, concentrated apple juice, concentrated loquat juice, concentrated grapefruit juice, concentrated peach juice, concentrated pear juice, passion fruit powder, blueberry powder, pomegranate powder, grapefruit powder, lemon powder, blood orange powder, apple powder, and pineapple powder; the food flavoring is selected from at least one of the following: compound berry flavoring, passion fruit flavoring, pomegranate flavoring, lemon flavoring, grape flavoring, grapefruit flavoring, loquat flavoring, pineapple flavoring, blueberry flavoring, lychee flavoring, and peach flavoring.

[0024] The preparation method of the above-mentioned oral liquid containing collagen peptides includes the following steps:

[0025] (a) Weigh each raw material according to the stated mass percentage, add collagen peptides, erythritol and sodium hyaluronate to water, heat to dissolve, and obtain mixture 1;

[0026] (b) Cool the mixture 1 to room temperature, add astaxanthin-ascorbic acid complex, γ-aminobutyric acid and concentrated pomegranate juice in sequence, homogenize under high pressure and then add xanthan gum and sodium carboxymethyl cellulose. Stir well to obtain mixture 2.

[0027] (c) Add lemon flavoring to mixture 2 and stir well. Filter, bottle, sterilize and seal to obtain the oral liquid containing collagen peptides.

[0028] Further, the heating temperature in step (a) is 60-70℃; the pressure of the high-pressure homogenization in step (b) is 20-25 MPa, the time is 1-1.5 min, and the number of times is 2-3.

[0029] Furthermore, the sterilization temperature in step (c) is 80-90°C, and the time is 10-15 min.

[0030] The above-mentioned oral liquid containing collagen peptides is used to prepare anti-aging products.

[0031] The present invention has the following advantages over the prior art:

[0032] 1. This invention provides an oral liquid containing collagen peptides, including collagen peptides, astaxanthin-ascorbic acid complex, and other ingredients. This invention chemically combines astaxanthin and ascorbic acid, improving the water solubility of astaxanthin and enhancing the stability of ascorbic acid, thereby strengthening the antioxidant capacity of both. The oral liquid prepared by combining astaxanthin and collagen peptides exhibits good stability, taste, and flavor. Long-term use can improve facial elasticity and facial skin condition.

[0033] 2. The present invention also provides a method for preparing the above-mentioned oral liquid containing collagen peptides, which is simple in process and provides a new idea and method for improving facial skin condition. Attached Figure Description

[0034] Figure 1 This is a schematic diagram of the preparation process of the astaxanthin-ascorbic acid complex of the present invention. Detailed Implementation

[0035] The technical solution of the present invention will be further described below with reference to specific embodiments. However, those skilled in the art should understand that the following embodiments are only for illustrating the present invention and should not be regarded as limiting the present invention. Specific conditions not specified in the embodiments are performed according to conventional conditions or conditions recommended by the manufacturer. Unless otherwise specified, the reagents or instruments used are all conventional products obtained through commercial channels. Example 1:

[0036] Example 1:

[0037] An oral liquid containing collagen peptides comprises the following ingredients by weight percentage: 20% fish collagen tripeptide, 0.8% astaxanthin-ascorbic acid complex, 0.15% sodium hyaluronate, 0.25% γ-aminobutyric acid, 6% erythritol, 0.2% xanthan gum, 0.6% sodium carboxymethyl cellulose, 2% concentrated pomegranate juice, 1% lemon flavoring, and the balance being water; the structural formula of the astaxanthin-ascorbic acid complex is as follows:

[0038] ;

[0039] The preparation process of the above astaxanthin-ascorbic acid complex is as follows, and the reaction process is as follows: Figure 1 As shown:

[0040] (1) Astaxanthin (1 mmol) and succinic anhydride (8 mmol) were added to dioxane (50 mL), and then triethylamine (3 mmol) was added as a catalyst. The reaction was carried out at 55 °C for 18 h. After the reaction was completed, 0.1 mol / L hydrochloric acid solution was added to the reaction solution until precipitation was complete. The precipitate was collected and purified by silica gel column chromatography. The precipitate was eluted with a gradient of chloroform-methanol (100:0 / 90:10 / 0:100, v / v). The eluents were combined and the solvent was evaporated to obtain intermediate 1.

[0041] Intermediate 1 1 H NMR (C 48 H 60 O 10 400 MHz d 6 -DMSO) δ 12.16 (s, 2H), 6.51 (d,10H), 6.24 (d, 4H), 5.45 (t, 2H), 2.71 (t, 4H), 2.53 (t, 4H), 2.36 (s, 6H), 2.14 (s, 12H), 1.70-1.68 (m, 2H), 1.46-1.44 (m, 2H), 1.25 (s, 12H); HRMS (ESI + ): [M+H] + The calculated value is 797.42, and the obtained value is 797.40; the above results confirm that the obtained product is the target product.

[0042] (2) Intermediate 1 (10 mmol), 4-formylphenylboronic acid (25 mmol) and triethylamine (25 mmol) were added to acetic anhydride (55 mL) and reacted at 90 °C for 6 h. After the reaction was completed, the reaction solution was cooled to room temperature, and diethyl ether (25 mL) and water (20 mL) were added, resulting in the precipitation of a solid precipitate. The organic phase was collected after filtration and extracted with 12% NaOH aqueous solution (3 × 10 mL). The aqueous phases were combined and concentrated hydrochloric acid was added to pH=2. The precipitate was collected, washed with water, and dried to obtain intermediate 2.

[0043] Intermediate 2 1 H NMR (C 62 H 70 B2O 14 400 MHz d 6-DMSO) δ 12.20 (s, 2H), 7.71(d,4H), 7.35(d, 4H), 7.01(s, 2H), 6.51 (d, 10H), 6.24 (d, 4H), 5.45 (t, 2H),4.28 (s, 4H), 3.56 (s, 4H), 2.36 (s, 6H), 2.14 (s, 12H), 1.70-1.68 (m, 2H),1.46-1.44 (m, 2H), 1.25 (s, 12H); HRMS (ESI + ): [M+H] + The calculated value is 1061.50, and the obtained value is 1061.47; the above results confirm that the obtained product is the target product.

[0044] (3) Add intermediate 2 (10 mmol) to sodium bicarbonate solution (0.15 mol / L, 110 mL), then add ascorbic acid (35 mmol) and stir for 20 h. After the reaction is completed, the reaction solution is purified by column chromatography to obtain the astaxanthin-ascorbic acid complex.

[0045] Astaxanthin-ascorbic acid complex 1 H NMR (C 74 H 76 B2O 22 Na2, 400 MHz, d 6 -DMSO) δ 7.71(d, 4H), 7.35(d, 4H), 7.01(s, 2H), 6.51 (d, 10H), 6.24 (d, 4H), 5.78(s, 2H),5.45 (t, 2H), 4.81 (d, 2H), 4.24-4.22(m, 2H), 3.96(s, 2H), 3.59-3.53(m, 4H),3.06 (s, 4H), 2.36 (s, 6H), 2.14 (s, 12H), 1.70-1.68 (m, 2H), 1.46-1.44 (m,2H), 1.25 (s, 12H); HRMS (ESI + ): [M+H] + The calculated value is 1385.48, and the obtained value is 1385.52; the above results confirm that the obtained product is the target product.

[0046] This embodiment also provides a method for preparing the oral liquid containing collagen peptides, the specific process of which is as follows:

[0047] (a) Weigh each raw material according to the above mass percentage, add fish collagen tripeptide, erythritol and sodium hyaluronate to water, stir at 65°C until dissolved, to obtain mixture 1;

[0048] (b) Cool the mixture 1 to room temperature, add astaxanthin-ascorbic acid complex, γ-aminobutyric acid and concentrated pomegranate juice in sequence, homogenize at 22 MPa 3 times, 1 min each time, then add xanthan gum and sodium carboxymethyl cellulose, stir for 30 min until uniform, to obtain mixture 2.

[0049] (c) Add lemon flavoring to mixture 2 and stir well. After filtration, fill into oral liquid bottles, pasteurize at 85°C for 12 min, cool and seal to obtain the oral liquid containing collagen peptides.

[0050] Example 2:

[0051] An oral liquid containing collagen peptides is composed of the following ingredients in weight percentage: 10% fish collagen tripeptide, 0.5% astaxanthin-ascorbic acid complex, 0.05% sodium hyaluronate, 0.1% γ-aminobutyric acid, 3% erythritol, 0.02% gum arabic, 0.03% pectin, 6% concentrated blueberry juice, 0.05% passion fruit flavor, and the balance being water; the structural formula of the astaxanthin-ascorbic acid complex is the same as in Example 1;

[0052] The preparation process of the above astaxanthin-ascorbic acid complex is as follows, and the reaction process is as follows: Figure 1 As shown:

[0053] (1) Astaxanthin (1 mmol) and succinic anhydride (5 mmol) were added to dioxane (50 mL), and then triethylamine (2 mmol) was added as a catalyst. The reaction was carried out at 50 °C for 20 h. After the reaction was completed, 0.1 mol / L hydrochloric acid solution was added to the reaction solution until precipitation was complete. The precipitate was collected and purified by silica gel column chromatography. The elution was carried out by gradient elution with chloroform-methanol (100:0 / 90:10 / 0:100, v / v). The eluents were combined and the solvent was evaporated to obtain intermediate 1. The NMR and mass spectrometry results of intermediate 1 were the same as those in Example 1.

[0054] (2) Intermediate 1 (10 mmol), 4-formylphenylboronic acid (20 mmol) and triethylamine (20 mmol) were added to acetic anhydride (50 mL) and reacted at 85 °C for 7 h. After the reaction was completed, the reaction solution was cooled to room temperature, and diethyl ether (25 mL) and water (20 mL) were added, resulting in the precipitation of a solid precipitate. The organic phase was collected after filtration and extracted with 12% NaOH aqueous solution (3 × 10 mL). The aqueous phases were combined and concentrated hydrochloric acid was added to pH=2. The precipitate was collected, washed with water, and dried to obtain intermediate 2. The NMR and mass spectrometry results of intermediate 2 were the same as in Example 1.

[0055] (3) Add intermediate 2 (10 mmol) to sodium bicarbonate solution (0.1 mol / L, 120 mL), then add ascorbic acid (30 mmol) and stir for 18 h. After the reaction, the reaction solution is purified by column chromatography to obtain the astaxanthin-ascorbic acid complex. The NMR and mass spectrometry results of the astaxanthin-ascorbic acid complex are the same as in Example 1.

[0056] This embodiment also provides a method for preparing the oral liquid containing collagen peptides, the specific process of which is as follows:

[0057] (a) Weigh each raw material according to the above mass percentage, add fish collagen tripeptide, erythritol and sodium hyaluronate to water, stir at 60°C until dissolved, to obtain mixture 1;

[0058] (b) Cool the mixture 1 to room temperature, add astaxanthin-ascorbic acid complex, γ-aminobutyric acid and concentrated pomegranate juice in sequence, homogenize at 20 MPa 3 times, 1.5 min each time, then add xanthan gum and sodium carboxymethyl cellulose, stir for 30 min until uniform, to obtain mixture 2.

[0059] (c) Add lemon flavoring to mixture 2 and stir well. After filtration, fill into oral liquid bottles, pasteurize at 80°C for 15 min, cool and seal to obtain the oral liquid containing collagen peptides.

[0060] Example 3:

[0061] An oral liquid containing collagen peptides is composed of the following ingredients in weight percentage: 30% fish collagen tripeptide, 1.2% astaxanthin-ascorbic acid complex, 0.3% sodium hyaluronate, 0.5% γ-aminobutyric acid, 8% erythritol, 1.0% locust bean gum, 0.1% passion fruit powder, 2% grape flavoring, and the balance being water; the structural formula of the astaxanthin-ascorbic acid complex is the same as in Example 1;

[0062] The preparation process of the above astaxanthin-ascorbic acid complex is as follows, and the reaction process is as follows: Figure 1 As shown:

[0063] (1) Astaxanthin (1 mmol) and succinic anhydride (10 mmol) were added to dioxane (50 mL), and then triethylamine (4 mmol) was added as a catalyst. The reaction was carried out at 60 °C for 15 h. After the reaction was completed, 0.1 mol / L hydrochloric acid solution was added to the reaction solution until precipitation was complete. The precipitate was collected and purified by silica gel column chromatography. The elution was carried out by gradient elution with chloroform-methanol (100:0 / 90:10 / 0:100, v / v). The eluents were combined and the solvent was evaporated to obtain intermediate 1. The NMR and mass spectrometry results of intermediate 1 were the same as those in Example 1.

[0064] (2) Intermediate 1 (10 mmol), 4-formylphenylboronic acid (30 mmol) and triethylamine (30 mmol) were added to acetic anhydride (60 mL) and reacted at 95 °C for 5 h. After the reaction was completed, the reaction solution was cooled to room temperature, and diethyl ether (25 mL) and water (20 mL) were added, resulting in the precipitation of a solid precipitate. The organic phase was collected after filtration and extracted with 12% NaOH aqueous solution (3 × 10 mL). The aqueous phases were combined and concentrated hydrochloric acid was added to pH=2. The precipitate was collected, washed with water, and dried to obtain intermediate 2. The NMR and mass spectrometry results of intermediate 2 were the same as in Example 1.

[0065] (3) Add intermediate 2 (10 mmol) to sodium bicarbonate solution (0.2 mol / L, 100 mL), then add ascorbic acid (40 mmol) and stir for 24 h. After the reaction is completed, the reaction solution is purified by column chromatography to obtain the astaxanthin-ascorbic acid complex. The NMR and mass spectrometry results of the astaxanthin-ascorbic acid complex are the same as in Example 1.

[0066] This embodiment also provides a method for preparing the oral liquid containing collagen peptides, the specific process of which is as follows:

[0067] (a) Weigh each raw material according to the above mass percentage, add fish collagen tripeptide, erythritol and sodium hyaluronate to water, stir at 70°C until dissolved, to obtain mixture 1;

[0068] (b) Cool the mixture 1 to room temperature, add astaxanthin-ascorbic acid complex, γ-aminobutyric acid and concentrated pomegranate juice in sequence, homogenize at 25 MPa twice for 1 min each time, then add xanthan gum and sodium carboxymethyl cellulose, stir for 30 min until uniform, to obtain mixture 2.

[0069] (c) Add lemon flavoring to mixture 2 and stir well. After filtration, fill into oral liquid bottles, pasteurize at 90°C for 10 min, cool and seal to obtain the oral liquid containing collagen peptides.

[0070] Comparative Example 1:

[0071] Comparative Example 1 is basically the same as Example 1, except that the astaxanthin-ascorbic acid complex in Example 1 is replaced with astaxanthin.

[0072] Comparative Example 2:

[0073] Comparative Example 2 is basically the same as Example 1, except that the astaxanthin-ascorbic acid complex in Example 1 is replaced with ascorbic acid.

[0074] Comparative Example 3:

[0075] Comparative Example 3 is basically the same as Example 1, except that the astaxanthin-ascorbic acid complex in Example 1 is replaced with a mixture of astaxanthin and ascorbic acid, and the amounts of astaxanthin and ascorbic acid are the same as in Example 1.

[0076] Experimental Example 1: Stability Test of Oral Solution

[0077] After the oral liquids obtained in Examples 1-3 and Comparative Examples 1-3 were left at room temperature for two weeks, it was observed whether the oral liquids in the bottles showed any stratification or precipitation. Meanwhile, 10 volunteers were selected to evaluate the taste and aroma of the oral liquids obtained in Examples 1-3 and Comparative Examples 1-3. The results are shown in Table 1.

[0078] Table 1: ;

[0079] As shown in Table 1, the oral liquids obtained in Examples 1-3 of this invention exhibit good stability, showing no stratification or precipitation after being left at room temperature for two weeks, and possessing a good flavor and refreshing taste. Compared to Example 1, the stability, taste, and odor of Comparative Examples 1-3 are inferior. This indicates that the present invention, by chemically combining astaxanthin and ascorbic acid, not only improves the water solubility of astaxanthin but also enhances the stability of ascorbic acid, thus maximizing the antioxidant capacity of both.

[0080] Experimental Example 2: Antioxidant Activity Test

[0081] The oral liquids containing collagen peptides prepared in Examples 1-3 and Comparative Examples 1-3 were tested for antioxidant activity using the following methods:

[0082] Prepare a 0.1 mmol / L DPPH solution using anhydrous ethanol.

[0083] Add 100 μL of 0.1 mmol / L DPPH solution to a transparent tube, then add 100 μL of anhydrous ethanol solution and mix well. Incubate in the dark for 40 min, and measure the absorbance of the mixture at 517 nm, which is recorded as B0.

[0084] Add 100 μL of 0.1 mmol / L DPPH solution to a transparent tube, then add 100 μL of each group of oral solutions and mix well. Incubate in the dark for 40 min, and measure the absorbance of the mixture at 517 nm. Record this value as B1.

[0085] Add 100 μL of anhydrous ethanol to a transparent tube, then add 100 μL of each group of oral solutions and mix well. Incubate in the dark for 40 min, and measure the absorbance of the mixture at 517 nm. Record this value as B2.

[0086] DPPH free radical scavenging rate = {1 - [(B1 - B2) / B0]} × 100%, and the results of DPPH free radical scavenging rate are shown in Table 2.

[0087] Table 2:

[0088] ;

[0089] As shown in Table 2, the oral liquids of Examples 1-3 exhibit good antioxidant activity. Compared with Example 1, the antioxidant activity of the oral liquids obtained in Comparative Example 1 (where the astaxanthin-ascorbic acid complex was replaced with astaxanthin), Comparative Example 2 (where the astaxanthin-ascorbic acid complex was replaced with ascorbic acid), and Comparative Example 3 (where the astaxanthin-ascorbic acid complex was replaced with a mixture of astaxanthin and ascorbic acid) decreased. This indicates that the present invention, by chemically combining astaxanthin and ascorbic acid, can significantly improve the stability of ascorbic acid and the water solubility of astaxanthin, thereby enabling it to fully exert its antioxidant capacity.

[0090] Experimental Example 3: Skin Elasticity Test

[0091] Seventy female volunteers aged 18-50 years with healthy, undamaged skin were selected and randomly divided into seven groups of ten participants each. Six groups received the oral solutions corresponding to Examples 1-3 and Comparative Examples 1-3, respectively, while the seventh group served as a blank control group, receiving water. The dosage was 5g / bottle, taken once daily with warm water before breakfast or dinner. Follow-up was conducted before use and again after two months of use, at the same time each day. No other products of the same type were allowed during the trial. Before the trial, the skin elasticity parameters of the participants' cheeks were measured using a Cutometer MPA580. The probe was used to measure the same area three times, and the average value was taken. Two months after each group of participants took the samples, their cheeks were measured again using the Cutometer MPA580.

[0092] The skin elasticity parameter R2 is the ratio of the skin's rebound amount without negative pressure to its maximum stretch amount under negative pressure. The closer R2 is to 1, the better the skin's elasticity. Based on the test parameter R2, the average change rate of R2 is calculated as follows: Average change rate of R2 (%) = [(R2 value of skin after 2 months of product use / R2 value of skin before product use) / R2 value of skin before product use] × 100%. The test results are shown in Table 3.

[0093] Table 3:

[0094] ;

[0095] As can be seen from Table 3, after taking the oral liquid obtained in Examples 1-3, the average change rate of skin R2 was relatively high, and the skin elasticity parameter R2 value of the cheek area increased by more than 17.14%, indicating that the oral liquid of the present invention has the effect of improving the condition of facial skin.

[0096] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them. The basic principles and main features of the present invention have been described above with specific implementation schemes. Based on the present invention, some modifications or substitutions can be made, but these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of protection claimed by the present invention.

Claims

1. An oral liquid containing collagen peptides, characterized in that, The product comprises the following components by weight percentage: collagen peptides 10-30%, astaxanthin-ascorbic acid complex 0.5-1.2%, sodium hyaluronate 0.05-0.3%, γ-aminobutyric acid 0.1-0.5%, erythritol 3-8%, stabilizer 0.05-1.0%, concentrated fruit juice or fruit powder 0.1-6.0%, food flavoring 0.05-2.0%, and the balance being water; the structural formula of the astaxanthin-ascorbic acid complex is as follows: ； The preparation process of the astaxanthin-ascorbic acid complex is as follows: (1) Astaxanthin and succinic anhydride were added to dioxane, and then triethylamine was added to react. After the reaction was completed, the reaction solution was purified to obtain intermediate 1. The structural formula of intermediate 1 is as follows: ； (2) Intermediate 1, 4-formylphenylboronic acid and triethylamine were added to acetic anhydride and reacted under heating conditions. The reaction solution was post-treated to obtain intermediate 2. The structural formula of intermediate 2 is: ； (3) Add intermediate 2 to a 0.1-0.2 mol / L sodium bicarbonate solution, then add ascorbic acid and stir to react. After the reaction is completed, the reaction solution is treated to obtain the astaxanthin-ascorbic acid complex.

2. The oral liquid containing collagen peptides according to claim 1, characterized in that, The molar ratio of astaxanthin, succinic anhydride and triethylamine in step (1) is 1:(5-10):(2-4); the reaction temperature is 50-60℃ and the time is 15-20 h.

3. The oral liquid containing collagen peptides according to claim 1, characterized in that, In step (2), the molar ratio of intermediate 1,4-formylphenylboronic acid and triethylamine is 1:(2-3):(2-3); the heating temperature is 85-95℃; and the reaction time is 5-7 h.

4. The oral liquid containing collagen peptides according to claim 1, characterized in that, In step (3), the ratio of intermediate 2, ascorbic acid and sodium bicarbonate solution is 1 mmol: (3-4) mmol: (10-12) mL; the reaction time is 18-24 h.

5. The oral liquid containing collagen peptides according to claim 1, characterized in that, The collagen peptide is a fish collagen tripeptide; the molecular weight of the collagen peptide is 500-1000 Da; the molecular weight of the sodium hyaluronate is 8-40 wDa; the stabilizer is selected from at least one of xanthan gum, sodium carboxymethyl cellulose, pectin, gum arabic, locust bean gum, and konjac gum.

6. The method for preparing the oral liquid containing collagen peptides according to any one of claims 1-5, characterized in that, Includes the following steps: (a) Weigh each raw material according to the stated mass percentage, add collagen peptides, erythritol and sodium hyaluronate to water, heat to dissolve, and obtain mixture 1; (b) Cool the mixture 1 to room temperature, add astaxanthin-ascorbic acid complex, γ-aminobutyric acid and concentrated pomegranate juice in sequence, homogenize under high pressure and then add xanthan gum and sodium carboxymethyl cellulose. Stir well to obtain mixture 2. (c) Add lemon flavoring to mixture 2 and stir well. Filter, bottle, sterilize and seal to obtain the oral liquid containing collagen peptides.

7. The method for preparing an oral liquid containing collagen peptides according to claim 6, characterized in that, The heating temperature in step (a) is 60-70℃; the pressure of the high-pressure homogenization in step (b) is 20-25 MPa, the time is 1-1.5 min, and the number of times is 2-3.

8. The method for preparing an oral liquid containing collagen peptides according to claim 6, characterized in that, The sterilization temperature in step (c) is 80-90℃ and the time is 10-15 min.

9. The application of the oral liquid containing collagen peptides according to any one of claims 1-5, characterized in that, Used in the preparation of anti-aging products.

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