Stenotrophomonas maltophilia for preventing and treating plant root-knot nematode and application of stenotrophomonas maltophilia

The bacterial agent prepared by the fermentation broth of Stenotrophomonas maltophilia Z5W solves the problem of plant root-knot nematode prevention and control, achieves the effect of highly effective killing of root-knot nematodes, and reduces the risk of plant diseases.

CN120648585APending Publication Date: 2025-09-16YUNNAN UNIV
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Patent Information

Application Number
CN202510569409.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-05-02
Publication Date
2025-09-16

AI Technical Summary

Technical Problem

Existing technologies are rarely used in the prevention and control of plant root-knot nematodes, and there is a lack of efficient prevention and control measures.

Method used

We provide Stenotrophomonas maltophilia Z5W and its fermentation liquid, which are used to prepare bacterial agents for controlling plant root-knot nematodes. The fermentation liquid has a 24-hour mortality rate of over 99% against root-knot nematodes, and even after being diluted 50 times, the mortality rate is still over 85%.

Benefits of technology

The fermentation liquid has a highly effective lethal effect on root-knot nematodes, can effectively prevent nematode larvae from entering plants, and reduce the incidence of plant diseases, and has good application prospects.

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Abstract

The invention discloses stenotrophomonas maltophilia for preventing and treating plant root-knot nematodes and application of the stenotrophomonas maltophilia. The stenotrophomonas maltophilia Z5W is preserved in the Guangdong Microbial Culture Collection Center, the number is GDMCC No: 65868, and the preservation address is No.100, Xianlie Middle Road, Xiaozhou District, Guangzhou City, Guangdong Province. The application of the stenotrophomonas maltophilia Z5W is the application of the stenotrophomonas maltophilia Z5W in prevention and treatment of the plant root-knot nematode, and particularly, the plant root-knot nematode is prevented and treated through a metabolite produced by culturing the stenotrophomonas maltophilia Z5W. The 24-hour fatality rate of the fermentation stock solution of stenotrophomonas maltophilia provided by the invention on root-knot nematode exceeds 99%, and the 12-hour fatality rate of the fermentation stock solution of stenotrophomonas maltophilia on root-knot nematode exceeds 78%; the mortality rate of the fermentation liquor diluted by 50 times to the root-knot nematodes exceeds 85%, the action time does not exceed 24 hours, the larvae of the root-knot nematodes can be effectively prevented from entering plants to form diseases, and the bacillus subtilis has a good application prospect in prevention and treatment of the plant root-knot nematodes.
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Claims

1. A strain of Stenotrophomonas maltophilia ( Stenotrophomonas maltophilia )Z5W ， It is deposited in Guangdong Provincial Microbiological Culture Collection with the number GDMCC No: 65868, and the deposit address is No. 100, Xianlie Middle Road, Yuexiu District, Guangzhou City, Guangdong Province.

2. Use of the Stenotrophomonas maltophilia Z5W according to claim 1 in preventing and controlling plant root-knot nematodes.

3. The application according to claim 2, characterized in that: The application is to control plant root-knot nematodes by using the metabolites produced by culturing Stenotrophomonas maltophilia Z5W.

4. A bacterial agent prepared based on the Stenotrophomonas maltophilia Z5W according to claim 1.

5. The bacterial agent prepared by Stenotrophomonas maltophilia Z5W according to claim 4, characterized in that The preparation method of the bacterial agent is as follows: 1) Stenotrophomonas maltophilia Z5W was transferred to a seed culture medium, and then cultured on a shaker at 35°C and 150-200 rpm for 20 h to obtain a fermentation seed solution; 2) The fermentation seed liquid is inoculated into the fermentation medium and cultured on a shaker at 33-35°C and 150-185 r / min for 48-72 hours. The resulting fermentation liquid is the target bacterial agent.

6. The bacterial agent prepared by Stenotrophomonas maltophilia Z5W according to claim 5, characterized in that In step 1), the seed culture medium is LB culture medium.

7. The bacterial agent prepared by Stenotrophomonas maltophilia Z5W according to claim 5, characterized in that In step 2), the fermentation medium is LB medium.

8. Use of the bacterial agent according to claim 4 in controlling root-knot nematodes in plants.

Citation Information

Patent Citations

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