Liquid culture medium for culturing high-efficiency compound bacterial system for compost and application of liquid culture medium

Abstract

The invention provides a liquid culture medium for culturing an efficient compound bacterium system for compost and application of the liquid culture medium, and belongs to the technical field of microorganism culture. The liquid culture medium comprises a basic culture medium for microbial culture and the following components: organic solid waste, an autoinducer 2 (AI-2), N-acyl homoserine lactone (AHLs), vitamin B1, biotin and L-cysteine. The liquid culture medium takes an organic solid waste as a target degradation product as a carbon source; aI-2 and AHLs are used as signal molecules; vitamin B1, biotin and L-cysteine are used as growth factors; through the synergistic effect of the characteristic carbon source, the signal molecules and the growth factors in the microorganism culture medium, the culture of the high-efficiency compound microbial system for composting containing non-culturable microorganisms in an inoculation source is realized, the number of available functional strains in an organic solid waste composting system is greatly increased and increased, and the interaction effect and the degradation efficiency of the strains are enhanced.

Claims

1. A liquid culture medium of a high-efficiency composite bacterial system for composting organic solid waste, characterized in that: The invention comprises a basic culture medium for microbial culture and components with the following concentrations: 5-10 g / L of organic solid waste, 10-50 μg / L of autoinducer-2, 1-5 μg / L of N-acylhomoserine lactone, 0.3-1 mg / L of vitamin B1, 0.1-0.4 mg / L of biotin and 20-40 mg / L of L-cysteine; the pH value of the liquid culture medium is 6.5-7.

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2. The liquid culture medium according to claim 1, characterized in that The liquid culture medium also includes by-products of organic solid waste composting; the by-products of composting include compost leachate.

3. The liquid culture medium according to claim 2, characterized in that The liquid culture medium comprises components with the following concentrations: 7.5-10 g / L of organic solid waste, 30-50 μg / L of AI-2, 3-5 μg / L of N-acyl homoserine lactone, 0.5-1 mg / L of vitamin B1, 0.2-0.4 mg / L of biotin, and 20-40 mg / L of L-cysteine; and the concentration of the compost leachate in the liquid culture medium is 50-100 mL / L.

4. The liquid culture medium according to any one of claims 1 to 3, characterized in that The organic solid waste includes one or more of kitchen waste, straw, landscaping waste and livestock and poultry manure.

5. Use of the liquid culture medium according to any one of claims 1 to 4 in at least one of the following: screening, enrichment, cultivation and utilization of high-efficiency composite bacterial strains for composting.

6. The use according to claim 5, characterized in that The high-efficiency composite bacterial system for composting includes composite functional microorganisms; the composite functional microorganisms include one or more species at the genus levels of Bacillus, Parageobacillus, Sulfurovum, Gordonia, Georgiania, Pseudomonas and Thiobacillus.

7. The use according to claim 5 or 6, characterized in that The composite functional microorganisms include microbial flora related to organic matter degradation and transformation and / or microbial flora related to greenhouse and malodorous gas regulation; the microbial flora related to organic matter degradation and transformation includes one or more of the flora at the genus levels of Bacillus, Pseudomonas and Parageobacillus; the microbial flora related to greenhouse and malodorous gas regulation includes one or more of the flora at the genus levels of Sulfurovum, Gordonia and Lactobacillus.

8. A high-efficiency composite bacterial system for composting, characterized in that: Obtained by culturing in the liquid culture medium according to any one of claims 1 to 4.

9. A method for screening, enriching and / or cultivating a high-efficiency composite bacterial system for composting, characterized in that: include: Inoculating the mature compost product into the liquid culture medium according to any one of claims 1 to 4 for culturing to obtain a culture containing a highly efficient composite bacterial system for composting; The compost raw materials of the compost mature product and the target degradation products of the high-efficiency composite bacterial system for composting are consistent in type.

10. Use of the high-efficiency composite bacterial system for composting according to claim 8 or the culture obtained by the method according to claim 9 in composting organic solid waste.

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