Taxodium Zhongshanha EIN3 protein, coding gene thereof and application of Taxodium Zhongshanha EIN3 protein in regulation of plant root development

Abstract

The invention provides a taxodium Zhongshanha EIN3 protein, a coding gene thereof and application of the taxodium Zhongshanha EIN3 protein in regulation and control of plant root development, and belongs to the technical field of plant genetic engineering. The invention provides a taxodium Zhongshanha EIN3 protein. The amino acid sequence is shown as SEQ ID NO: 1. The taxodium Zhongshanha EIN3 protein has a negative regulation effect in regeneration of adventitious roots of woody plants. The embodiment shows that along with the development of adventitious roots of the taxodium Zhongshanha, the expression level of the ThEIN3 is gradually reduced, and the expression quantity of the ThEIN3 in the adult taxodium Zhongshanha cutting slips is obviously higher than that of the rejuvenation cutting slips; compared with WT, the development of adventitious roots of ThEIN3 overexpression plants is inhibited, the content of gibberellin is remarkably increased, the content of auxin and trans-zeatin nucleoside is reduced, and it is speculated that Taxodium Zhongshanha EIN3 protein can inhibit the development of the adventitious roots by regulating the hormone level. The invention provides high-quality molecular resources for taxodium Zhongshanha molecular breeding, and has important application value in the field of forest tree genetic engineering.

Claims

1. A Zhongshan fir EIN3 protein, characterized in that The amino acid sequence is shown in SEQ ID NO:

1.

2. A gene encoding the EIN3 protein of Cunninghamia lanceolata, characterized in that: The nucleotide sequence is shown in SEQ ID NO:

2.

3. A gene derivative comprising the gene according to claim 2, characterized in that: Includes at least one of the following products: expression cassette, recombinant vector and recombinant bacteria.

4. Use of the Zhongshan Fir EIN3 protein of claim 1, the gene of claim 2, or the gene derivative of claim 3 for at least one of the following: regulating plant root development, regulating plant hormone levels, and cultivating transgenic plant varieties; The plants include woody plants and / or herbaceous plants.

5. The application according to claim 4, characterized in that: The method for regulating plant root development is that the Zhongshan Fir EIN3 protein, the gene or the gene derivative negatively regulates plant root development; The root system includes adventitious roots.

6. The application according to claim 4, characterized in that: The plant hormone comprises at least one of auxin, trans-zeatin riboside and gibberellin; The method for regulating plant hormone levels comprises at least one of the following: The Zhongshan Fir EIN3 protein, the gene or the gene derivative positively regulates the gibberellin level of the plant; The C. zhongshanense EIN3 protein, the gene or the gene derivative negatively regulates the auxin level and / or trans-zeatin riboside level of the plant.

7. A method for regulating plant adventitious root development, characterized in that: Regulating the expression level of the Zhongshan Fir EIN3 protein described in claim 1 or the gene described in claim 2 in a plant.

8. A method for evaluating the adventitious root development ability of a plant, characterized in that: The relative expression level of the Zhongshan Fir EIN3 protein according to claim 1 or the gene according to claim 2 in the plant is detected, and the relative expression level is inversely proportional to the adventitious root regeneration ability of the plant.

9. A method for plant cuttings, characterized in that: The relative expression level of the Zhongshan fir EIN3 protein according to claim 1 or the gene according to claim 2 in the plant is detected, and cuttings with inferior relative expression levels are selected for cutting.

10. The method according to claim 9, characterized in that: The relative expression level detection reagent includes a forward primer having a nucleotide sequence as shown in SEQ ID NO: 3 and a reverse primer having a nucleotide sequence as shown in SEQ ID NO: 4.

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