Bio-enzyme preparation cleaning method and system

Abstract

The invention discloses a biological enzyme preparation cleaning method and system.The method comprises the following steps that 1, glucose is added into a compound enzyme premixing tank, a mixed solution is prepared, the mixed solution is sprayed into a granulation drying machine, glucose oxidase is contained in the compound enzyme premixing tank, and the granulation drying machine is started; 2, spraying the catalase solution in the catalase tank body into the granulation drying machine so as to carry out secondary spray washing on the granulation drying machine, carrying out secondary spray washing on the granulation drying machine, and carrying out secondary spray washing on the granulation drying machine, the catalase effectively decomposes hydrogen peroxide; step 3, spraying the alkaline protease solution in the alkaline protease tank body into the granulation drying machine so as to carry out third spray washing on the granulation drying machine; and step 4, spraying the reverse osmosis water in the reverse osmosis water tank into the granulation drying machine so as to carry out fourth spray washing on the granulation drying machine. The invention aims to realize efficient cleaning of the granulating dryer.

Description

Technical Field

[0001] The present invention relates to the technical field of equipment cleaning, and in particular to a method and system for cleaning a biological enzyme preparation. Background Art

[0002] Currently, granulation dryers are widely used in the pharmaceutical and food industries. Their complex internal structures are prone to residual contaminants such as sugars, oils, and proteins. Traditional cleaning methods rely on manual operation and use a single alkaline protease or chemical agent for cleaning. Existing liquid mixed enzymes often contain protease, but protease will decompose other biological enzymes (such as lipase and amylase), resulting in the inactivation of the complex enzyme cleaning system and low enzyme cleaning efficiency. Existing enzyme cleaning can only decompose organic matter and cannot kill microorganisms such as bacteria and spores, which can easily lead to excessive total colony counts in products and lack of sterilization function. The internal pipes of the granulation dryer are tortuous and the nozzles are densely distributed. Traditional low-pressure, fixed-angle nozzles have difficulty covering dead corners such as the capture chamber and the base, resulting in residual cleaning blind spots. The cleaning process requires manual mixing of reagents, switching of pipes, and monitoring time, which is time-consuming and prone to omissions. The cleaning consistency is poor and it is highly dependent on manual operation.

[0003] The existing invention patent with the publication number CN109234045A discloses a medical multi-enzyme cleaning solution and its preparation method. The cleaning solution allows protease and other enzymes such as lipase, catalase, ligninase and glucose oxidase to coexist through component matching, while reducing the overall enzyme usage and reducing costs. Although the decomposition of other enzymes by protease is inhibited by surfactants (such as polyoxypropylene glycerol ether, glycerol polyoxypropylene ether) and stabilizers (pullulan), the enzyme activity may gradually decrease due to oxidation, temperature changes or microbial contamination during long-term storage. And this solution is mainly aimed at medical pollutants such as blood and human secretions, but the cleaning effect on other common industrial pollutants such as oils, sugars, and proteins has not been clearly verified, and there may be limitations on the scope of application. Summary of the Invention

[0004] The main purpose of the present invention is to provide a bio-enzyme preparation cleaning method and system, aiming to solve the technical problem that the existing cleaning method cannot efficiently clean the granulation dryer.

[0005] To achieve the above-mentioned object, the present invention provides a bio-enzyme preparation cleaning method, which is applied to a bio-enzyme preparation cleaning system, wherein the system includes a granulation dryer and a bio-enzyme preparation tank body connected to the granulation dryer, wherein the granulation dryer is connected to the bio-enzyme preparation tank body through a main pipeline, and the bio-enzyme preparation tank body includes a complex enzyme premix tank body, a catalase tank body, an alkaline protease tank body, and a reverse osmosis water tank body, wherein the complex enzyme premix tank body, the catalase tank body, the alkaline protease tank body, and the reverse osmosis water tank body are respectively connected to the main pipeline, and the method comprises the following steps:

[0006] Step 1: Glucose is added to a complex enzyme premix tank to prepare a mixed solution, and the mixed solution is sprayed into various areas inside a granulation dryer through a main pipe to perform a first spray-wash of the granulation dryer, and the glucose and glucose oxidase inside the granulation dryer react to produce hydrogen peroxide, so that the hydrogen peroxide can disinfect and sterilize the inside of the granulation dryer, wherein the activity ratio of liquid amylase, cellulase, lipase, and glucose oxidase in the complex enzyme premix tank is (1-2):(1-2):(3-5):1, and the ratio of the total mass of the mixed solution of amylase, cellulase, lipase, and glucose oxidase to the mass of glucose is (8-10):1;

[0007] Step 2: spraying the catalase solution in the catalase tank into various areas inside the granulation dryer through the main pipe to perform a second spray wash of the granulation dryer, so that the catalase can effectively decompose hydrogen peroxide, wherein the activity ratio of the catalase solution in the catalase tank to the glucose oxidase is (20-40):3;

[0008] Step 3, spraying the alkaline protease solution in the alkaline protease tank into various areas inside the granulation dryer through the main pipe to perform a third spray wash of the granulation dryer, wherein the activity ratio of the alkaline protease solution to the catalase solution is 3:(2-4);

[0009] Step 4: The reverse osmosis water in the reverse osmosis water tank is sprayed into various areas inside the granulating dryer through the main pipe to perform the fourth spray washing of the granulating dryer.

[0010] Optionally, the granulation dryer comprises a capturing chamber, a granulation chamber and a base from top to bottom, and the capturing chamber, the granulation chamber and the base are respectively connected to the bio-enzyme preparation tank through a main pipeline.

[0011] Optionally, a first branch pipe, a second branch pipe, and a third branch pipe are respectively arranged between the capture chamber, the granulation chamber, and the base and the main pipe, and a rotating nozzle is respectively installed at the end of the first branch pipe, the second branch pipe, and the third branch pipe;

[0012] Optionally, corresponding first switch valves, second switch valves, third switch valves, and fourth switch valves are installed between the complex enzyme premix tank, catalase tank, alkaline protease tank, and reverse osmosis water tank and the main line, and each first switch valve, second switch valve, third switch valve, and fourth switch valve is respectively connected to the PLC system electrical signal.

[0013] Optionally, a booster pump is further installed in the main line, and the booster pump is electrically connected to the PLC system.

[0014] Optionally, corresponding first regulating valves, second regulating valves, and third regulating valves are respectively installed in the first branch pipeline, the second branch pipeline, and the third branch pipeline, and the first regulating valves, the second regulating valves, and the third regulating valves are electrically connected to the PLC system.

[0015] Optionally, in step 1, the liquid amylase activity is 600-1200 U / ml, the cellulase activity is 600-1200 U / ml, the lipase activity is 1800-3000 U / ml, and the glucose oxidase activity is 600 U / ml. The four are prepared in proportion to form a 20 kg mixed solution, and 2 kg of glucose is added.

[0016] Optionally, spray for 10 minutes in steps 1-3 respectively, then pause, soak and react for 15 minutes, and then wash for 5 minutes in step 4.

[0017] In addition, in order to achieve the above-mentioned purpose, the present invention also provides a bio-enzyme preparation cleaning system, which includes a granulation dryer and a bio-enzyme preparation tank connected to the granulation dryer, the granulation dryer includes a capture chamber, a granulation chamber and a base from top to bottom, the capture chamber, granulation chamber and base are respectively connected to the bio-enzyme preparation tank through a main pipeline, a booster pump is installed in the main pipeline, the bio-enzyme preparation tank includes a complex enzyme premix tank, a catalase tank, an alkaline protease tank and a reverse osmosis water tank, the complex enzyme premix tank, the catalase tank, the alkaline protease tank and the reverse osmosis water tank are respectively connected to the main pipeline through the corresponding first switch valve, second switch valve, third switch valve and fourth switch valve.

[0018] Optionally, the boost pump, the first switch valve, the second switch valve, the third switch valve, and the fourth switch valve are respectively connected to the PLC system electrical signal.

[0019] Beneficial effects:

[0020] The bioenzyme preparation cleaning method of the present invention uses liquid amylase, cellulase, lipase, glucose oxidase, and glucose to spray-clean the capture chamber, material preparation chamber, and base of the granulation dryer, then spray-cleans with catalase alone, and finally spray-clean with alkaline protease alone. Cleaning in a specific order and at a specific time can prevent alkaline protease from decomposing other enzymes, thereby completely decomposing dirt such as sugars, oils, and proteins. In addition, during the first enzyme washing step, because glucose oxidase and glucose are sprayed in all directions, glucose and glucose oxidase react under aerobic conditions to produce low-concentration hydrogen peroxide. By generating hydrogen peroxide in situ, the interior of the granulation dryer can be disinfected and sterilized in an all-round manner. At the same time, polysaccharide hydrolases such as amylase and cellulase are added in the first enzyme washing step. The small molecule sugars generated after these amylases and cellulases hydrolyze the sugar dirt can also serve as substrates for the subsequent action of glucose oxidase. By increasing the substrate concentration, hydrogen peroxide generation is promoted, thereby enhancing the disinfection effect. After sterilization, a catalase spray is used to decompose any residual hydrogen peroxide, preventing it from entering the product. Finally, the interior of the device is rinsed with reverse osmosis water for a thorough clean. The entire cleaning process is precisely controlled, requiring minimal water and electricity consumption, and is environmentally friendly in a short time.

[0021] And a bio-enzyme preparation cleaning system, which is achieved by connecting the granulation dryer with the bio-enzyme preparation tank, the granulation dryer includes a capture chamber, a granulation chamber and a base from top to bottom, the capture chamber, the granulation chamber and the base are respectively connected to the bio-enzyme preparation tank through a main pipeline, the main pipeline is equipped with a booster pump, the bio-enzyme preparation tank includes a complex enzyme premix tank, a catalase tank, an alkaline protease tank and a reverse osmosis water tank, so that the complex enzyme premix tank, the catalase tank, the alkaline protease tank and the reverse osmosis water tank in the bio-enzyme preparation tank can clean the capture chamber, the granulation chamber and the base respectively, avoiding the decomposition of other enzymes by protease, and the cost is low. In addition, a pneumatic diaphragm valve is installed on each tank and nozzle, and all are connected to the PLC system of the granulation dryer, so that the spraying time of each tank can be set separately, and automatic cleaning can be achieved as required, achieving one-button cleaning and realizing intelligent cleaning. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is a flow chart of an embodiment of a method for cleaning a biological enzyme preparation according to the present invention;

[0023] Figure 2 This is a structural schematic diagram of an embodiment of a biological enzyme preparation cleaning system of the present invention.

[0024] Explanation of Figure Numbers

[0025] 1. Complex enzyme premix tank, 2. Catalase tank, 3. Alkaline protease tank, 4. Reverse osmosis water tank, 5. Main pipeline, 6. First branch pipeline, 7. Second branch pipeline, 8. Third branch pipeline, 101. First on / off valve, 201. Second on / off valve, 301. Third on / off valve, 401. Fourth on / off valve, 501. Variable frequency booster pump, 601. First regulating valve, 701. Second regulating valve, 801. Third regulating valve, a. Base, c. Capture chamber, b. Granulation chamber

[0026] The purpose, features and advantages of the present invention will be further described with reference to the accompanying drawings and in conjunction with the embodiments. DETAILED DESCRIPTION

[0027] It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0028] See also Figure 1-2 The present invention provides a bio-enzyme preparation cleaning method, which is applied to a bio-enzyme preparation cleaning system, such as Figure 2 As shown, the system includes a granulation dryer and a bio-enzyme preparation tank connected to the granulation dryer, the granulation dryer is connected to the bio-enzyme preparation tank via a main line 5, and the dryer body includes a capture chamber c, a granulation chamber b, and a base a distributed from top to bottom, the capture chamber c, the granulation chamber b, and the base a are respectively connected to the main line 5 via corresponding branch lines, and a variable frequency booster pump 501 is installed in the main line 5. The bio-enzyme preparation tank includes a complex enzyme premix tank 1, a catalase tank 2, an alkaline protease tank 3, and a reverse osmosis water tank 4, and the complex enzyme premix tank 1, the catalase tank 2, the alkaline protease tank 3, and the reverse osmosis water tank 4 are respectively connected to the main line 501. The complex enzyme premix tank 1 is loaded with liquid amylase, cellulase, lipase, and glucose oxidase.

[0029] Furthermore, the complex enzyme premix tank 1, catalase tank 2, alkaline protease tank 3, and reverse osmosis water tank 4 are connected to the main pipeline 501 via the corresponding first switch valve 101, second switch valve 201, third switch valve 301, and fourth switch valve 401, respectively. Preferably, the variable frequency booster pump 501, the first switch valve 101, the second switch valve 201, the third switch valve 301, and the fourth switch valve 401 are each electrically connected to the PLC system, thereby realizing intelligent control of each tank through the PLC system. Preferably, the PLC system is configured with a timing module and a sequence control module, which are electrically connected to each switch valve to trigger the opening and closing operations of each tank in sequence according to preset time nodes.

[0030] Furthermore, corresponding liquid level sensors are arranged inside the complex enzyme premix tank 1, the catalase tank 2, the alkaline protease tank 3 and the reverse osmosis water tank 4. Each liquid level sensor is connected to the PLC system electrical signal to provide real-time feedback on the liquid level inside each tank, so as to further adjust the opening of the corresponding switch valve.

[0031] The PLC system also includes a feedback control module, which receives signals from the liquid level and pressure sensors of each receiving tank in real time and dynamically adjusts the speed of the variable-frequency booster pump 501 and the opening of the corresponding regulating valve. For example, if a nozzle clog causes a sudden pressure surge and the pressure sensor 51 detects that the pressure exceeds the maximum value, the PLC triggers an emergency response, immediately reducing the speed of the variable-frequency booster pump 501 to its minimum speed and simultaneously opening the corresponding regulating valve to 100%, thereby reducing the main line pressure by diverting the flow. If the pressure still exceeds the standard, the PLC closes the switch valve and triggers an alarm. Alternatively, if the tank liquid level is too low and the corresponding liquid level sensor detects that the level is below 20%, the PLC executes protection logic, reducing the speed of the variable-frequency booster pump 501 to the minimum speed required to maintain pressure and closing the corresponding switch valve. PLC timing control and pressure feedback control reduce manual intervention and operational errors. Furthermore, variable-frequency speed regulation and valve fine-tuning reduce energy consumption by 30% and minimize mechanical wear and tear on high-pressure flushing equipment.

[0032] Furthermore, a first branch pipe 6, a second branch pipe 7, and a third branch pipe 8 are respectively arranged between the capture chamber, the granulation chamber, and the base and the main pipe, and a rotary nozzle is installed at the end of each of the first branch pipe 6, the second branch pipe 7, and the third branch pipe 8. Preferably, each rotary nozzle is a multi-degree-of-freedom oscillating nozzle with a coverage angle of 120-360° and a nozzle diameter of 0.5-1.2 mm. More preferably, each rotary nozzle is also linked to the PLC system electrical signal, that is, the PLC system drives the motor and angle adjustment structure of the rotary nozzle through the output module, and indirectly controls the start and stop, speed or angle of the rotary nozzle. And each rotary nozzle is also provided with an anti-corrosion, anti-static and heat-dissipating shell on the outside.

[0033] Furthermore, the first branch pipe 6, the second branch pipe 7, and the third branch pipe 8 are respectively equipped with a corresponding first regulating valve 601, a second regulating valve 701, and a third regulating valve 801. The first regulating valve 601, the second regulating valve 701, and the third regulating valve 801 are electric regulating valves, and their opening and closing states are independently controlled by the PLC system through the electromagnetic valve group according to the preset timing. Furthermore, by setting the spraying time of the solution in each tank separately, it can be automatically cleaned as required to achieve one-button cleaning. That is, when the granulating dryer needs to be cleaned, enter the PLC control panel, click the one-button cleaning button, and various materials, valves, nozzles, booster pumps, etc. will be cleaned in coordination with the set parameters.

[0034] Furthermore, the opening of the electric regulating valve is dynamically adjusted by the PLC according to the pressure (P) and flow (Q) of the main line 5, which satisfies:

[0035] (k is the pipeline characteristic coefficient).

[0036] Furthermore, the first on-off valve 101 , the second on-off valve 201 , the third on-off valve 301 , and the fourth on-off valve 401 are all pneumatic diaphragm valves, and their opening and closing states are controlled by a PLC system.

[0037] Furthermore, the method comprises the following steps:

[0038] In step 1, glucose is added to the complex enzyme premix tank 1 to form a mixed solution. The mixed solution is then sprayed into various areas within the granulation dryer via the main pipe 5 to perform the first spray-cleaning of the granulation dryer. The glucose and glucose oxidase react in situ on the surface of the stains on the inner wall of the granulation dryer to produce hydrogen peroxide, which disinfects and sterilizes the interior of the granulation dryer. The concentration of hydrogen peroxide produced in this step is 0.2-0.6%. This step uses a bioenzymatic method to produce hydrogen peroxide in situ, ensuring its stability at low concentrations and maintaining its disinfection and sterilization effectiveness. This addresses the inconveniences associated with conventional high-concentration hydrogen peroxide disinfection solutions. Hydrogen peroxide stability is affected by several factors: 1. Temperature: Temperature is a key factor affecting hydrogen peroxide decomposition. As temperature increases, the rate of hydrogen peroxide decomposition accelerates. At room temperature, the decomposition rate of hydrogen peroxide is slow, but at elevated temperatures, the decomposition reaction proceeds rapidly. 2. Light: Light also promotes the decomposition of hydrogen peroxide. Exposure to strong light, such as ultraviolet light, breaks the chemical bonds in hydrogen peroxide molecules, triggering a decomposition reaction. ③ Catalysts: Certain substances can accelerate the decomposition of hydrogen peroxide. These substances, such as metal ions and enzymes, are called catalysts. Under the action of catalysts, the decomposition rate of hydrogen peroxide is significantly increased. ④ Pressure: Pressure also has a certain impact on the decomposition of hydrogen peroxide. Under high pressure, the decomposition rate of hydrogen peroxide may decrease. Conversely, under reduced pressure or vacuum conditions, the decomposition of hydrogen peroxide may be accelerated. ⑤ Impurities: Impurities in hydrogen peroxide may also trigger its decomposition. Certain impurities can act as catalysts, promoting the decomposition of hydrogen peroxide. Therefore, these factors must be carefully controlled during storage and use. Furthermore, the low-concentration hydrogen peroxide produced in the above steps can also reduce the subsequent use of catalase after disinfection, reducing cleaning costs. The bioenzymatic method for preparing hydrogen peroxide is characterized by high efficiency, specificity, and safety.

[0039] Preferably, the activity ratio of the liquid amylase, cellulase, lipase, and glucose oxidase in the complex enzyme premix tank 1 is (1-2): (1-2): (3-5): 1, and the ratio of the total mass of the mixed solution of amylase, cellulase, lipase, and glucose oxidase to the mass of glucose is (8-10): 1;

[0040] Step 2: spraying the catalase solution in the catalase tank 2 into various areas inside the granulation dryer through the main line 501 to perform a second spray wash of the granulation dryer, so that the catalase can effectively decompose hydrogen peroxide, wherein the activity ratio of the catalase solution in the catalase tank 2 to the glucose oxidase is (20-40):3;

[0041] Step 3: spraying the alkaline protease solution in the alkaline protease tank 3 into various areas inside the granulation dryer through the main pipe 501 to perform a third spray wash of the granulation dryer, wherein the activity ratio of the alkaline protease solution to the catalase solution is 3:(2-4);

[0042] Step 4: The reverse osmosis water in the reverse osmosis water tank 4 is sprayed into various areas inside the granulating dryer through the main line 501 to perform the fourth spray washing of the granulating dryer.

[0043] Furthermore, in step 1, the liquid amylase activity is 1200 U / ml, the cellulase activity is 1200 U / ml, the lipase activity is 3000 U / ml, and the glucose oxidase activity is 600 U / ml. The four are proportionally prepared into a 20 kg mixed solution, and 2 kg of glucose is added. In step 2, the catalase solution activity is 8000 U / ml and the mass is 12 kg. In step 3, the alkaline protease solution activity is 6000 U / ml and the mass is 15 kg.

[0044] Furthermore, in actual use, the spraying time in step 1 is controlled to be 10 minutes, and in steps 2-4, the spraying time is controlled to be 5 minutes respectively, and there is a 15-minute pause between each step to allow for soaking reaction. The entire cleaning process is precisely controlled, uses less water and electricity, takes less time, and is environmentally friendly.

[0045] Granulating dryers are specialized stainless steel equipment used in continuous, long-term industrial enzyme production. Due to their numerous internal components, cleaning is time-consuming, labor-intensive, and often incomplete. Their interior walls are heavily fouled, with a pale yellow layer averaging 0.2-1 mm thick. The primary fouling components are dextrin, starch, cellulose, fat, and protein, biomolecules commonly used as bio-enzyme adjuvants. To address this heavily fouled interior, the one-touch enzymatic automatic descaling and cleaning method described in the following examples and comparative examples was employed.

[0046] Example 1

[0047] Step 1. Ingredients: First, mix 1200U / ml liquid amylase, 1200U / ml cellulase, 3000U / ml liquid lipase, and 600U / ml glucose oxidase in an activity ratio of 2:2:5:1 to prepare 20kg of a mixed product. Clean the compound enzyme premix tank #1 and add it to the mixture. Set aside. Add 2kg of glucose and dissolve it before spraying. Prepare 12kg of liquid catalase with an activity of 8000U / ml. Clean the catalase tank #2 and add it to the mixture. Set aside. Prepare 15kg of liquid alkaline protease with an activity of 6000U / ml. Clean the alkaline protease tank #3 and add it to the mixture. Set aside. Clean the reverse osmosis water tank #4 and add 180kg of reverse osmosis water to the mixture. Set aside.

[0048] Step 2, parameter setting: on the PLC control panel of the granulating dryer, set the time to 10 minutes to spray the materials on the 1# feed car, pause for 15 minutes, set the time to 5 minutes to spray the materials on the 2# feed car, set the time to 5 minutes to spray the materials on the 3# feed car, pause for 15 minutes, set the time to 5 minutes to spray the materials on the 4# feed car. The above time should be set once before cleaning.

[0049] Step 3: When the pelletizing dryer needs to be cleaned, access the PLC control panel and click the one-touch cleaning button. Various materials, valves, nozzles, and booster pumps will coordinately clean the interior of the equipment, spraying the material from the first feed car within 10 minutes, pausing for 15 minutes, spraying the material from the second feed car within 5 minutes, spraying the material from the third feed car within 5 minutes, pausing for 15 minutes, and spraying the material from the fourth feed car within 5 minutes. The internal dirt removal and microbial data of the pelletizing dryer will be tested after cleaning.

[0050] Example 2

[0051] Step 1. Ingredients: First, mix 1200U / ml liquid amylase, 1200U / ml cellulase, 3000U / ml liquid lipase, and 600U / ml glucose oxidase in an activity ratio of 2:2:3:1 to prepare 20kg of a mixed product. Clean the compound enzyme premix tank #1 and add it to the mixture. Set aside. Add 2kg of glucose and dissolve it before spraying. Prepare 12kg of liquid catalase with an activity of 8000U / ml. Clean the catalase tank #2 and add it to the mixture. Set aside. Prepare 15kg of liquid alkaline protease with an activity of 6000U / ml. Clean the alkaline protease tank #3 and add it to the mixture. Set aside. Clean the reverse osmosis water tank #4 and add 180kg of reverse osmosis water to the mixture. Set aside.

[0052] Step 2, parameter setting: on the PLC control panel of the granulating dryer, set the time to 10 minutes to spray the materials on the 1# feed car, pause for 15 minutes, set the time to 5 minutes to spray the materials on the 2# feed car, set the time to 5 minutes to spray the materials on the 3# feed car, pause for 15 minutes, set the time to 5 minutes to spray the materials on the 4# feed car. The above time should be set once before cleaning.

[0053] Step 3: When the pelletizing dryer needs to be cleaned, access the PLC control panel and click the one-touch cleaning button. Various materials, valves, nozzles, and booster pumps will coordinately clean the interior of the equipment, spraying the material from the first feed car within 10 minutes, pausing for 15 minutes, spraying the material from the second feed car within 5 minutes, spraying the material from the third feed car within 5 minutes, pausing for 15 minutes, and spraying the material from the fourth feed car within 5 minutes. The internal dirt removal and microbial data of the pelletizing dryer will be tested after cleaning.

[0054] Example 3

[0055] Step 1. Ingredients: First, mix 1200U / ml liquid amylase, 1200U / ml cellulase, 3000U / ml liquid lipase, and 600U / ml glucose oxidase in an activity ratio of 1:2:3:1 to prepare 20kg of a mixed product. Clean the compound enzyme premix tank #1 and add it to the mixture. Set aside. Add 2kg of glucose and dissolve it before spraying. Prepare 12kg of liquid catalase with an activity of 8000U / ml. Clean the catalase tank #2 and add it to the mixture. Set aside. Prepare 15kg of liquid alkaline protease with an activity of 6000U / ml. Clean the alkaline protease tank #3 and add it to the mixture. Set aside. Clean the reverse osmosis water tank #4 and add 180kg of reverse osmosis water to the mixture. Set aside.

[0056] Step 2, parameter setting: on the PLC control panel of the granulating dryer, set the time to 10 minutes to spray the materials on the 1# feed car, pause for 15 minutes, set the time to 5 minutes to spray the materials on the 2# feed car, set the time to 5 minutes to spray the materials on the 3# feed car, pause for 15 minutes, set the time to 5 minutes to spray the materials on the 4# feed car. The above time should be set once before cleaning.

[0057] Step 3: When the pelletizing dryer needs to be cleaned, access the PLC control panel and click the one-touch cleaning button. Various materials, valves, nozzles, and booster pumps will coordinately clean the interior of the equipment, spraying the material from the first feed car within 10 minutes, pausing for 15 minutes, spraying the material from the second feed car within 5 minutes, spraying the material from the third feed car within 5 minutes, pausing for 15 minutes, and spraying the material from the fourth feed car within 5 minutes. The internal dirt removal and microbial data of the pelletizing dryer will be tested after cleaning.

[0058] Furthermore, in order to better demonstrate the cleaning effect of the present invention, the following control test was conducted.

[0059] Comparative Example 1

[0060] Similar to steps 1 and 2 of Example 1, in step 3, the materials from carts 1-3 were simultaneously fed into the granulation dryer using the PLC control panel. The spraying time was controlled to 10 minutes, followed by a 15-minute pause, and the material from cart 4 was sprayed for a final 5 minutes. The dirt removal and microbial data inside the granulation dryer were then tested after cleaning.

[0061] Comparative Example 2

[0062] The process is substantially the same as steps 2 and 3 of Example 1, except that glucose oxidase is not added to the composite enzyme premix tank 1# feed car in step 1. After cleaning, the dirt removal and microbial data inside the granulating dryer are tested.

[0063] Comparative Example 3

[0064] The process was essentially the same as steps 2 and 3 of Example 1, except that in step 1, the amount of amylase used in the composite enzyme premix tank (cart 1) was reduced. 600 U / ml liquid amylase, 1200 U / ml cellulase, 3000 U / ml liquid lipase, and 1200 U / ml glucose oxidase were mixed at an activity ratio of 0.5:2:5:1. After cleaning, the internal dirt removal and microbial data of the granulating dryer were tested.

[0065] Comparative Example 4

[0066] The process was essentially the same as steps 2 and 3 of Example 1, except that in step 1, the amount of cellulase used in the composite enzyme premix tank (cart 1) was reduced. 1200 U / ml liquid amylase, 600 U / ml cellulase, 3000 U / ml liquid lipase, and 1200 U / ml glucose oxidase were mixed at an activity ratio of 2:0.5:5:1. After cleaning, the internal dirt removal and microbial data of the granulator dryer were tested.

[0067] Comparative Example 5

[0068] The process was essentially the same as steps 2 and 3 of Example 1, except that in step 1, the amount of lipase used in the composite enzyme premix tank (cart 1) was reduced. 1200 U / ml liquid amylase, 1200 U / ml cellulase, 1800 U / ml liquid lipase, and 1200 U / ml glucose oxidase were mixed at an activity ratio of 2:2:3:1. After cleaning, the internal dirt removal and microbial data of the granulating dryer were tested.

[0069] Comparative Example 6

[0070] The process was essentially the same as steps 2 and 3 of Example 1, except that in step 1, a mixture of liquid amylase, cellulase, lipase, and 3% by mass hydrogen peroxide solution was added directly to the first feed car of the complex enzyme premix tank. After cleaning, the dirt removal and microbial data inside the granulation dryer were tested.

[0071] Comparative Example 7

[0072] The process was substantially the same as steps 2 and 3 of Example 1, except that no catalase was added to the catalase tank 2# feed car in step 1. After cleaning, the dirt removal and microbial data inside the granulating dryer were tested.

[0073] Comparative Example 8

[0074] The steps 2 and 3 are substantially the same as those in Example 1, except that no alkaline protease is added to the alkaline protease tank 3# feed car in step 1. After the cleaning is completed, the dirt removal and microbial data inside the granulating dryer are tested.

[0075] For each example and comparative example, the final reverse osmosis water wash wastewater was collected and isolated and counted using a gradient dilution method, in accordance with the standard "Test Method for Lactic Acid Bacteria in Food for Import and Export" (SN / T 1941.1-2017). The removal of dirt from the inner wall of the granulator was evaluated by observation. The cleaning and disinfection results for each example and comparative example are shown in Table 1.

[0076] Table 1

[0077]

[0078]

[0079] From the above, it can be seen that Examples 1-3 adopt step-by-step cleaning, while in Comparative Example 1, all enzymes are added into the granulation dryer at the same time. After the final cleaning effect, the scale layer does not change significantly, dirt adheres to the inner wall, and the average number of microorganisms is 11899 cfu / ml. It can be seen that the composite enzyme step-by-step cleaning method of the present invention avoids protease degradation of other enzymes and can effectively remove dirt from the stainless steel granulator. In Comparative Example 2, glucose oxidase is not added, and thus glucose oxidase cannot react with glucose to generate hydrogen peroxide. After cleaning, although the scale layer disappears, the inner wall returns to the original metallic color of the stainless steel. However, a certain number of microorganisms still remained inside. This indicates that the glucose oxidase of the present invention reacts with glucose, effectively enhancing the effectiveness of low-concentration hydrogen peroxide in-situ disinfection and sterilization. Furthermore, in Comparative Examples 3-5, the dosages of amylase, cellulase, and lipase were reduced accordingly, resulting in sticky sugar or grease dirt on the inner wall, which easily bred a certain number of microorganisms. Furthermore, in Comparative Example 6, a mixture of a 3% hydrogen peroxide solution was directly used for cleaning. Despite the fact that cleaning with high-concentration hydrogen peroxide alone did not significantly change the scale layer, dirt adhered to the inner wall and also contained a certain number of microorganisms. In Comparative Example 7, no catalase was added. The hydrogen peroxide remaining in the tank denatured the subsequently added protease, reducing its activity and resulting in incomplete degradation of protein adherents and enzyme proteins introduced during the previous cleaning step, leaving a certain number of microorganisms still inside. Furthermore, in Comparative Example 8, no alkaline protease was added, and the protein dirt could not be thoroughly cleaned during the cleaning process, resulting in dirt adhered to the inner wall and ultimately containing a certain number of microorganisms.

[0080] It should be noted that, in this document, the terms "comprises," "includes," or any other variations thereof are intended to encompass non-exclusive inclusion, such that a process, method, article, or system comprising a series of elements includes not only those elements but also other elements not explicitly listed, or elements inherent to such process, method, article, or system. In the absence of further limitations, an element defined by the phrase "comprising a ..." does not exclude the presence of other identical elements in the process, method, article, or system comprising the element.

[0081] The serial numbers of the above embodiments of the present invention are for description only and do not represent the advantages or disadvantages of the embodiments.

[0082] The above are only preferred embodiments of the present invention and are not intended to limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made using the contents of the present invention description and drawings, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A method for cleaning a biological enzyme preparation, characterized in that: The method is applied to a bio-enzyme preparation cleaning system, which includes a granulation dryer and a bio-enzyme preparation tank connected to the granulation dryer, wherein the granulation dryer is connected to the bio-enzyme preparation tank via a main pipeline, and the bio-enzyme preparation tank includes a complex enzyme premix tank, a catalase tank, an alkaline protease tank, and a reverse osmosis water tank, wherein the complex enzyme premix tank, the catalase tank, the alkaline protease tank, and the reverse osmosis water tank are respectively connected to the main pipeline. The method comprises the following steps: Step 1: Glucose is added to a complex enzyme premix tank to prepare a mixed solution, and the mixed solution is sprayed into various areas inside a granulating dryer through a main pipe to perform a first spray-washing of the granulating dryer, wherein the complex enzyme premix tank is filled with liquid amylase, cellulase, lipase, and glucose oxidase with an activity ratio of (1-2):(1-2):(3-5):1, so that the glucose and glucose oxidase inside the granulating dryer react to produce hydrogen peroxide, and the hydrogen peroxide is used to disinfect and sterilize the inside of the granulating dryer, and the ratio of the total mass of the mixed solution of amylase, cellulase, lipase, and glucose oxidase to the mass of glucose is (8-10):1; Step 2: spraying the catalase solution in the catalase tank into various areas inside the granulation dryer through the main pipe to perform a second spray wash of the granulation dryer, so that the catalase can effectively decompose hydrogen peroxide, wherein the activity ratio of the catalase solution in the catalase tank to the glucose oxidase is (20-40):3; Step 3, spraying the alkaline protease solution in the alkaline protease tank into various areas inside the granulation dryer through the main pipe to perform a third spray wash of the granulation dryer, wherein the activity ratio of the alkaline protease solution to the catalase solution is 3:(2-4); Step 4: The reverse osmosis water in the reverse osmosis water tank is sprayed into various areas inside the granulating dryer through the main pipe to perform the fourth spray washing of the granulating dryer.

2. The bio-enzyme preparation cleaning method according to claim 1, wherein The granulation dryer comprises a collecting chamber, a granulation chamber and a base from top to bottom. The collecting chamber, the granulation chamber and the base are respectively connected with the bio-enzyme preparation tank through a main pipeline.

3. The bio-enzyme preparation cleaning method according to claim 2, wherein: A first branch pipe, a second branch pipe and a third branch pipe are respectively arranged between the capture chamber, the granulation chamber and the base and the main pipe, and rotating nozzles are respectively installed at the ends of the first branch pipe, the second branch pipe and the third branch pipe.

4. The bio-enzyme preparation cleaning method according to claim 1, wherein Corresponding first switch valves, second switch valves, third switch valves, and fourth switch valves are installed between the complex enzyme premix tank, catalase tank, alkaline protease tank, and reverse osmosis water tank and the main line, and each first switch valve, second switch valve, third switch valve, and fourth switch valve is respectively connected to an external PLC system electrical signal.

5. The bio-enzyme preparation cleaning method according to claim 1, wherein: A booster pump is also installed in the main pipe, and the booster pump is connected to an external PLC system electrical signal.

6. The bio-enzyme preparation cleaning method according to claim 3, wherein: The first branch pipeline, the second branch pipeline and the third branch pipeline are also respectively installed with corresponding first regulating valves, second regulating valves and third regulating valves, and the first regulating valves, the second regulating valves and the third regulating valves are connected to the external PLC system electrical signal.

7. The bio-enzyme preparation cleaning method according to any one of claims 1 to 6, characterized in that: In step 1, the liquid amylase activity is 600-1200 U / ml, the cellulase activity is 600-1200 U / ml, the lipase activity is 1800-3000 U / ml, and the glucose oxidase activity is 600 U / ml. The four are prepared into a 20 kg mixed liquid according to the proportion, and 2 kg of glucose is added.

8. The bio-enzyme preparation cleaning method according to claim 7, wherein: The spraying was performed for 10 minutes in step 1, and for 5 minutes in steps 2, 3, and 4, respectively. There was a 15-minute pause between adjacent steps to allow for soaking reaction.

9. A bio-enzyme preparation cleaning system used in the method according to any one of claims 1 to 8, characterized in that: The system includes a granulation dryer and a bio-enzyme preparation tank connected to the granulation dryer. The granulation dryer includes a capture chamber, a granulation chamber and a base distributed from top to bottom. The capture chamber, the granulation chamber and the base are respectively connected to the bio-enzyme preparation tank through a main pipeline. A booster pump is installed in the main pipeline. The bio-enzyme preparation tank includes a complex enzyme premix tank, a catalase tank, an alkaline protease tank and a reverse osmosis water tank. The complex enzyme premix tank, the catalase tank, the alkaline protease tank and the reverse osmosis water tank are respectively connected to the main pipeline through corresponding first, second, third and fourth switch valves.

10. The bio-enzyme cleaning system according to claim 9, characterized in that: The booster pump, the first switch valve, the second switch valve, the third switch valve, and the fourth switch valve are respectively connected to the PLC system electrical signal.

Images