Fertilizer, preparation method, use method and application

Abstract

The invention belongs to the technical field of agricultural chemistry, and particularly relates to a fertilizer, a preparation method, a use method and application. The invention provides the application of the ammonium nitrogen fertilizer in improving the salt and alkali resistance of inositol, the inositol can participate in the formation of ascorbic acid, remove excess free oxygen and relieve plant salt damage stress, and the inositol derivative PI4P improves the activity of plasma membrane ATP enzyme, strengthens an SOS1 signal channel and improves proton potential energy for excretion of sodium ions. Ammonium ions of the ammonium nitrogen fertilizer can strengthen the activity of SOS2, so that SOS1 salt elimination is regulated and controlled, plant rhizosphere acidification is promoted, higher proton potential energy is improved for sodium ion excretion, salt damage is relieved, and ammonium nitrogen can strengthen the salt-resistant effect of inositol. The invention also provides a fertilizer which takes inositol and ammonium nitrogen as core active components and is suitable for stress-resistant growth-promoting cultivation of crops in a salinized soil environment.

Description

Technical Field

[0001] The present invention belongs to the technical field of agricultural chemistry, and in particular relates to a fertilizer, a preparation method, a use method and an application thereof. Background Art

[0002] In the existing technology, conventional water-soluble fertilizers are prone to low nutrient utilization due to ion antagonism in salinized soils, and lack targeted regulatory functions for plant physiological disorders under salt stress. Traditional salt-resistant agents mostly focus on osmotic regulating substances (such as betaine and proline), but they are expensive and have insufficient long-term effectiveness. The development and product creation of functional water-soluble fertilizers are limited to the production of already developed amino acids, humic acid and seaweed extracts, and there is less development of other synergistic substances. The cost of humic acid and alginic acid is high, and how to improve the salt and alkali resistance of low-cost inositol is urgently needed. Summary of the Invention

[0003] The invention provides a fertilizer, a preparation method, a use method and an application thereof. The application of ammonium nitrogen fertilizer can improve the salt-alkali resistance of inositol.

[0004] In order to solve the above technical problems, the present invention proposes the following technical solutions:

[0005] The invention provides an application of an ammonium nitrogen fertilizer in improving the salt-alkali resistance of inositol.

[0006] Preferably, the ammonium nitrogen fertilizer comprises monoammonium phosphate.

[0007] The invention provides a base fertilizer comprising ammonium nitrogen fertilizer and inositol; the base fertilizer contains 1.2-1.6 g / L of ammonium nitrogen fertilizer and 0.4-0.8 g / L of inositol; and the pH value of the base fertilizer is 4-4.5.

[0008] The present invention provides a fertilizer, comprising independently packaged base fertilizer according to the above technical solution and independently packaged topdressing fertilizer;

[0009] The topdressing fertilizer comprises a first topdressing fertilizer and a second topdressing fertilizer which are separately packaged, wherein the first topdressing fertilizer contains 0.8-1.0 g / L of potassium fertilizer, 1.2-1.6 g / L of ammonium nitrogen fertilizer, 0.4-0.8 g / L of inositol and 5.5-6.5 g / L of amide nitrogen fertilizer;

[0010] The second topdressing fertilizer contains 0.8-1.0 g / L of potassium fertilizer, 1.2-1.6 g / L of ammonium nitrogen fertilizer, and 5.5-6.5 g / L of amide nitrogen fertilizer;

[0011] The amide nitrogen fertilizer includes urea.

[0012] Preferably, the topdressing fertilizer also contains macroelement fertilizer and trace element fertilizer; the macroelement fertilizer includes 0.0195-0.078 g / L magnesium fertilizer; the trace element fertilizer includes one or more of 0.004-0.016 g / L copper fertilizer, 0.0035-0.014 g / L manganese fertilizer, 0.00034-0.00136 g / L boron fertilizer and 0.00275-0.011 g / L iron fertilizer.

[0013] Preferably, the magnesium fertilizer includes magnesium sulfate, the copper fertilizer includes copper sulfate pentahydrate, the manganese fertilizer includes manganese sulfate monohydrate, the boron fertilizer includes boric acid, and the iron fertilizer includes EDTA-sodium iron.

[0014] The present invention provides the base fertilizer described in the above technical solution or the application of the fertilizer described in the above technical solution in one or more of the following 1) to 3),

[0015] 1) Plant growth promotion in saline-alkali environment;

[0016] 2) Promote plant nutrient absorption in saline-alkali environments;

[0017] 3) Improve the salt resistance of plants in saline-alkali environments.

[0018] The present invention provides a method for using the base fertilizer or the fertilizer according to the above technical solution, comprising: applying the base fertilizer or the topdressing fertilizer to plants grown in a saline-alkali environment.

[0019] Preferably, the plant includes corn; the application period of the base fertilizer includes the sowing period; and the application period of the topdressing fertilizer includes the seedling stage.

[0020] Preferably, the field application amount of the base fertilizer in the fertilizer is 200-400 mL per 2-4 plants; the field application amount of the topdressing fertilizer in the fertilizer is 200-400 mL per 2-4 plants.

[0021] Beneficial effects of the present invention: The present invention provides the use of ammonium nitrogen fertilizer in improving the salt-alkali resistance of inositol. Under saline-alkali conditions, the sodium ion content in plants increases, and inositol and ammonium nitrogen fertilizer can reduce the accumulation of sodium ions in plants, alleviate salt damage, and reduce salt stress. The results of the examples show that the combined use of inositol and ammonium nitrogen fertilizer significantly promoted the biomass of the aboveground and underground parts of corn under saline-alkali stress, achieving the effect of alleviating salt damage; the combined use of inositol and ammonium nitrogen fertilizer promoted the absorption of nitrogen, phosphorus, calcium, and zinc nutrients by corn in saline-alkali soil; the combined use of inositol and ammonium nitrogen fertilizer can alleviate the damage caused by salt and alkali to corn, reduce the content of malondialdehyde and hydrogen peroxide, protect cell membranes, and protect the cell osmotic balance by accumulating osmotic regulating substances such as soluble protein and soluble sugar.

[0022] The present invention also provides a base fertilizer comprising ammonium nitrogen fertilizer and inositol; the base fertilizer contains 1.2-1.6 g / L ammonium nitrogen fertilizer and 0.4-0.8 g / L inositol. The present invention also provides a fertilizer comprising an independently packaged base fertilizer and an independently packaged topdressing fertilizer; the topdressing fertilizer comprises potash fertilizer, ammonium nitrogen fertilizer, and amide nitrogen; the topdressing fertilizer contains 0.8-1.0 g / L potash fertilizer, 1.2-1.6 g / L ammonium nitrogen fertilizer, and 5.5-6.5 g / L amide nitrogen fertilizer; the amide nitrogen fertilizer comprises urea.

[0023] The base fertilizer and topdressing fertilizer of the present invention are functional water-soluble fertilizer products containing inositol for salt resistance and growth promotion. They have inositol and ammonium nitrogen as their core active ingredients. Ammonium nitrogen fertilizer can improve the salt-alkali resistance of inositol and is suitable for adversity-resistant and growth-promoting cultivation of crops in salinized soil environments. The topdressing fertilizer of the present invention provides nutrients for plant growth. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 The plant height measurement results of the corn seedlings in Example 1 are as follows;

[0025] Figure 2 The results of measuring the stem diameter of the corn seedlings in Example 1 are as follows;

[0026] Figure 3 The SPAD value of the corn seedlings in Example 1 is measured;

[0027] Figure 4 The leaf area measurement results of the corn seedlings in Example 1 are as follows;

[0028] Figure 5 The aboveground dry weight of the corn seedlings in Example 1 is measured;

[0029] Figure 6 The results of the appearance phenotypic measurement of the corn seedlings in Example 1; from left to right are CK, inositol 0.1 g / L, inositol 0.2 g / L, inositol 0.4 g / L, inositol 0.8 g / L, inositol 1.0 g / L, inositol 1.2 g / L;

[0030] Figure 7 These are the measurement results of the aboveground fresh weight, underground fresh weight and aboveground dry weight of the corn seedlings of Comparative Example 1; the aboveground fresh weight in the figure represents the aboveground fresh weight of each plant, and the underground fresh weight represents the underground fresh weight of each plant, the same below.

[0031] Figure 8 The plant height, stem diameter and SPAD value of the corn seedlings in Comparative Example 1 are measured;

[0032] Figure 9The underground dry weight of the corn seedlings in Comparative Example 1 is measured; the underground dry weight represents the underground dry weight of each plant; the same below;

[0033] Figure 10 The results of the plant height, stem diameter and SPAD value of corn seedlings in Example 2 are as follows;

[0034] Figure 11 The aboveground dry weight and aboveground dry weight of the corn seedlings in Example 2 are measured; the aboveground dry weight represents the aboveground dry weight of each plant;

[0035] Figure 12 The results of the phenotypic determination of corn seedlings in Example 3 are shown on the left. The results for medium and low saline-alkali soils are shown. CK, T1, T2, and T3 for medium and low saline-alkali soils represent low-CK, low-T1, low-T2, and low-T3, respectively. The results for high saline-alkali soils are shown on the right. CK, T1, T2, and T3 for high saline-alkali soils represent high-CK, high-T1, high-T2, and high-T3, respectively.

[0036] Figure 13 The results of plant height, stem diameter and SPAD value measurement of corn seedlings in Example 3 are as follows;

[0037] Figure 14 The leaf area, underground dry weight and aboveground dry weight of corn seedlings were measured in Example 3.

[0038] Figure 15 The results of the aboveground nitrogen, phosphorus, and potassium content measurements of corn seedlings in Example 3 are shown in the figure on the far left, the aboveground nitrogen content results in the middle, and the aboveground potassium content results on the right.

[0039] Figure 16 The results of the aboveground calcium, magnesium, and zinc content measurements of corn seedlings in Example 3 are shown in the figure on the far left, the aboveground calcium content results in the middle, and the aboveground zinc content results on the right.

[0040] Figure 17 The results of rhizosphere pH, relative conductivity, and hydrogen peroxide content measurement of corn seedlings in Example 3 are shown, with the leftmost figure showing the rhizosphere pH result, the middle figure showing the hydrogen peroxide content result, and the right figure showing the relative conductivity result.

[0041] Figure 18 The figures are the malondialdehyde, soluble protein, and soluble sugar content determination results of corn seedlings in Example 3, wherein the leftmost figure is the malondialdehyde content result, the middle figure is the soluble protein content result, and the right figure is the soluble sugar content result;

[0042] Figure 19 The plant height, SPAD value, stem diameter, leaf area, aboveground dry weight, and underground dry weight of corn seedlings in Example 4 are measured.

[0043] Figure 20 These are the results of measuring the malondialdehyde, hydrogen peroxide, soluble protein, and soluble sugar contents in the aerial parts of corn seedlings in Example 4;

[0044] Figure 21 The results of the determination of nitrogen, phosphorus, potassium, zinc, calcium, and magnesium contents in the aboveground parts of corn seedlings in Example 4 are as follows;

[0045] Figure 22 This is the growth condition of the corn seedling potted in Example 4;

[0046] Figure 23 The figure shows the dissolution results of the water-soluble trace element fertilizer and inositol of Example 5; Figures 7-11 、 Figures 13-21 Different letters indicate significant differences at the P < 0.05 level. DETAILED DESCRIPTION

[0047] The invention provides application of ammonium nitrogen fertilizer in improving the salt-alkali resistance of inositol.

[0048] As an optional embodiment, the ammonium nitrogen fertilizer of the present invention includes monoammonium phosphate. The inositol of the present invention can effectively improve the salt-alkali resistance of plants and has low cost.

[0049] Under saline-alkali stress, sodium ions enter the plant body and inhibit plant growth. Inositol promotes the synthesis of plant ascorbic acid to remove reactive oxygen species (ROS) in the plant body, reduce plant cell loss, and alleviate the effects of salt damage. Inositol can also generate inositol derivatives phosphatidylinositol 4-phosphate (PI4P), which can promote the activity of ATPase to promote the release of hydrogen ions in the plant body, leading to the excretion of sodium ions in the plant body. When plant roots absorb ammonium nitrogen, acidification occurs, releasing hydrogen ions, and the high proton gradient is the plasma membrane Na + / H + The sodium ion efflux of the reverse transport protein SOS1 provides proton potential energy, promoting sodium ion efflux. Ammonium nitrogen acts as a synergist, where ammonium strengthens the serine / threonine protein kinase SOS2 signaling pathway, promoting the SOS1 signaling pathway to efflux sodium ions from the plant body, alleviating salt damage. At the same time, ammonium nitrogen can also promote plant rhizosphere acidification, thereby increasing the hydrogen ion concentration outside the root surface cells, forming a higher proton potential energy difference to directly regulate the activity of the transport protein SOS1 and promote sodium ion efflux. It can be seen that ammonium nitrogen and inositol synergistically enhance the salt resistance. The results of the examples show that inositol and monoammonium phosphate can alleviate the damage caused by salt and alkali to corn, reduce the content of malondialdehyde and hydrogen peroxide, and protect the cell membrane; at the same time, they maintain cell osmotic balance by accumulating the content of osmotic regulating substances such as soluble protein and soluble sugar; and promote the absorption of nitrogen, phosphorus, calcium and zinc nutrients by corn in saline-alkali soil.

[0050] The present invention provides a base fertilizer comprising ammonium nitrogen fertilizer and inositol, wherein the base fertilizer contains 1.2 to 1.6 g / L of ammonium nitrogen fertilizer and 0.4 to 0.8 g / L of inositol. The technical features of the ammonium nitrogen fertilizer of the present invention have been discussed above and will not be repeated here.

[0051] The base fertilizer provided by the present invention contains 1.2-1.6 g / L ammonium nitrogen fertilizer, or 1.3-1.5 g / L, more preferably 1.376 g / L. Ammonium nitrogen fertilizer supplements the nitrogen and phosphorus elements required for plant growth and alleviates plant salt damage.

[0052] The base fertilizer provided by the present invention contains 0.4 to 0.8 g / L inositol, or 0.4 to 0.6 g / L. The ammonium nitrogen and inositol in the fertilizer of the present invention improve the salt-alkali resistance of plants, and are suitable for stress-resistant and growth-promoting cultivation of crops in salinized soil environments. The inositol of the present invention can be extracted from discarded corn pulp, and the cost is 60 yuan / kg, which is low. The salt-resistant fertilizer of the present invention can significantly reduce costs compared to adding salt-resistant substances such as humic acid and alginic acid. Inositol is an effective carbon source for soil microorganisms, and its application can help plant microorganisms to stabilize and increase the number of beneficial microorganisms, thereby achieving the goal of improving the environmental level of regional soil microorganisms.

[0053] As an optional implementation manner, the base fertilizer uses deionized water as a solvent, and the water is deionized water or tap water.

[0054] The method for preparing a base fertilizer according to the present invention comprises: mixing an ammonium nitrogen fertilizer and inositol to obtain the base fertilizer. As an optional embodiment, the mixing temperature is 40°C to 60°C, and the solvent used in the mixing comprises deionized water. The mixing method comprises stirring. The present invention does not particularly limit the stirring parameters; conventional methods can be used. The temperature of 40°C to 60°C is selected to promote the dissolution of the ammonium nitrogen fertilizer and inositol.

[0055] The base fertilizer of the present invention has a pH of 4 to 4.5. Adjusting the pH to 4 to 4.5 can locally acidify the rhizosphere soil, converting insoluble phosphates into soluble H2PO4-, and achieving controllable neutralization with saline-alkali soil buffer systems (such as CaCO3). The fertilizer components of the present invention are all water-soluble and have excellent stability.

[0056] The present invention provides a fertilizer, comprising the independently packaged base fertilizer according to the above technical solution and the independently packaged topdressing fertilizer.

[0057] The technical features of the base fertilizer of the present invention have been discussed above and will not be repeated here.

[0058] As an optional embodiment, the topdressing fertilizer uses deionized water as a solvent, and the water is deionized water or tap water. The pH of the topdressing fertilizer is 4 to 4.5. Adjusting the pH to 4 to 4.5 can locally acidify the rhizosphere soil, convert insoluble phosphates into soluble H2PO4-, and form a controllable neutralization with the saline-alkali soil buffer system (such as CaCO3). The various components of the fertilizer of the present invention are water-soluble substances with good stability. The topdressing fertilizer of the present invention includes a first topdressing fertilizer and a second topdressing fertilizer that are packaged separately.

[0059] The first topdressing fertilizer of the present invention contains 1.2-1.6 g / L of ammonium nitrogen fertilizer, 0.4-0.8 g / L of inositol, 0.8-1.0 g / L of potash fertilizer, and 5.5-6.5 g / L of amide nitrogen fertilizer. The first topdressing fertilizer provided by the present invention contains 1.2-1.6 g / L of ammonium nitrogen fertilizer, and may also contain 1.3-1.5 g / L, more preferably 1.376 g / L.

[0060] The first topdressing fertilizer provided by the present invention contains 0.8-1.0 g / L of potash fertilizer, or 0.9-0.95 g / L, more preferably 0.92 g / L. The potash fertilizer of the present invention includes potassium sulfate, which meets the potash fertilizer requirements of plants.

[0061] The first topdressing fertilizer provided by the present invention contains 5.5-6.5 g / L of amide nitrogen fertilizer, or 5.8-6.3 g / L, more preferably 6.16 g / L. The amide nitrogen fertilizer of the present invention includes urea, which meets the nitrogen fertilizer requirements of plants.

[0062] The first topdressing fertilizer provided by the present invention contains 0.4 to 0.8 g / L inositol, or 0.4 to 0.6 g / L. The role of inositol has been discussed above and will not be repeated here. The first topdressing fertilizer is added with inositol and is only applied during the first topdressing after emergence. The inositol of the present invention is divided into two applications. The first application is base fertilizer, which is applied during the sowing period to help seed germination and resist salt stress; the second application is the first topdressing in the seedling stage after emergence. The application of inositol after emergence can help the seedlings alleviate salt damage, improve resistance, and establish a good plant structure in the early stage of the plant so that they can develop better.

[0063] The second topdressing fertilizer of the present invention contains 1.2-1.6 g / L of ammonium nitrogen fertilizer, 0.8-1.0 g / L of potash fertilizer, and 5.5-6.5 g / L of amide nitrogen fertilizer. The second topdressing fertilizer provided by the present invention includes 1.2-1.6 g / L of ammonium nitrogen fertilizer, and can also be 1.3-1.5 g / L, more preferably 1.376 g / L. The role of ammonium nitrogen fertilizer has been discussed above and will not be repeated here.

[0064] The second topdressing fertilizer provided by the present invention contains 0.8-1.0 g / L of potash fertilizer, or 0.9-0.95 g / L, more preferably 0.92 g / L. The potash fertilizer of the present invention includes potassium sulfate, which meets the potash fertilizer requirements of plants.

[0065] The second topdressing fertilizer provided by the present invention contains 5.5 to 6.5 g / L of amide nitrogen fertilizer, and can also contain 5.8 to 6.3 g / L, more preferably 6.16 g / L. The amide nitrogen fertilizer of the present invention includes urea, which meets the nitrogen fertilizer requirements of plants. The second topdressing fertilizer does not contain inositol and is only applied during the second topdressing after seedling emergence and subsequent topdressing.

[0066] As an optional embodiment, the first and second topdressing fertilizers of the present invention further comprise a macronutrient fertilizer and a trace element fertilizer, respectively. As an optional embodiment, the macronutrient fertilizer of the present invention comprises 0.0195 to 0.078 g / L of magnesium fertilizer; and the magnesium fertilizer of the present invention comprises magnesium sulfate.

[0067] As an optional embodiment, the trace element fertilizer of the present invention includes one or more of 0.004-0.016 g / L copper fertilizer, 0.0035-0.014 g / L manganese fertilizer, 0.00034-0.00136 g / L boron fertilizer, and 0.00275-0.011 g / L iron fertilizer. The copper fertilizer of the present invention includes copper sulfate pentahydrate. The manganese fertilizer of the present invention includes manganese sulfate monohydrate. The boron fertilizer of the present invention includes boric acid. The iron fertilizer of the present invention includes EDTA-sodium iron.

[0068] The topdressing fertilizer of the present invention supplements the nutrients required for plant growth and promotes plant growth. Inositol can further promote the absorption of macroelements and trace elements by plants.

[0069] The preparation method of the first topdressing fertilizer of the present invention comprises: mixing the ammonium nitrogen fertilizer, inositol, potassium fertilizer, amide nitrogen fertilizer, macronutrient fertilizer and trace element fertilizer to obtain topdressing fertilizer. The preparation method of the second topdressing fertilizer of the present invention comprises: mixing the ammonium nitrogen fertilizer, potassium fertilizer, amide nitrogen fertilizer, macronutrient fertilizer and trace element fertilizer to obtain topdressing fertilizer. As an optional embodiment, the mixing temperature of the present invention is 40°C to 60°C, the solvent used in the mixing comprises deionized water; and the mixing method comprises stirring. The present invention does not specifically limit the parameters of the stirring, and conventional methods can be used. The temperature of 40 to 60°C is selected to promote the dissolution of ammonium nitrogen fertilizer, potassium fertilizer, amide nitrogen fertilizer, macronutrient fertilizer and trace element fertilizer.

[0070] The present invention has no special limitation on the sources of the components of the base fertilizer and topdressing fertilizer, and conventional products can be used.

[0071] The present invention provides the use of the base fertilizer described in the above technical solution or the fertilizer described in the above technical solution in promoting plant growth in a saline-alkali environment. As an optional embodiment, the plant growth promotion indicators of the present invention include one or more of plant height, stem diameter, SPAD, leaf area, aboveground dry weight and underground dry weight. The results of the examples show that fertilizers containing inositol and monoammonium phosphate significantly improve the absorption of nitrogen, phosphorus, potassium, calcium and zinc nutrients in saline-alkali soil by corn. The technical features of the saline-alkali environment described in the present invention have been discussed above and will not be repeated here.

[0072] The present invention provides the use of the base fertilizer described in the above technical solution or the fertilizer described in the above technical solution in promoting plant nutrient absorption in a saline-alkali environment. The technical features of the saline-alkali environment described in the present invention have been discussed above and will not be repeated here. As an optional embodiment, the nutrients described in the present invention include one or more of nitrogen, phosphorus, calcium and zinc. The results of the examples show that the fertilizer containing inositol and monoammonium phosphate significantly promotes the absorption of nitrogen, phosphorus, potassium, calcium and zinc nutrients in saline-alkali soil by corn.

[0073] The present invention provides the use of the base fertilizer described in the above technical solution or the fertilizer described in the above technical solution in improving the salt resistance of plants in a saline-alkali environment. The technical features of the saline-alkali environment described in the present invention have been discussed above and will not be repeated here. The method of improving the salt resistance of plants described in the present invention includes the use of one or more of the following: reducing the content of malondialdehyde in plants, reducing the content of hydrogen peroxide in plants, increasing the content of soluble protein in plants, increasing the content of soluble sugar in plants, and reducing the electrolyte exudation rate in plants. Fertilizers containing inositol and monoammonium phosphate can alleviate the damage caused by salt and alkali to corn, significantly reduce the content of malondialdehyde and hydrogen peroxide, and protect cell membranes; reduce the electrolyte exudation rate, and at the same time protect the cell osmotic balance by accumulating the content of osmotic regulating substances such as soluble protein and soluble sugar.

[0074] The present invention provides a method for using the base fertilizer or the fertilizer according to the above technical solution, comprising: applying the base fertilizer or the fertilizer to plants planted in a saline-alkali environment. As an optional embodiment, the plants according to the present invention include corn. The base fertilizer and the topdressing fertilizer in the fertilizer according to the present invention are applied separately. The application period of the base fertilizer according to the present invention includes the sowing period; the number of applications of the base fertilizer is preferably 1. The application period of the topdressing fertilizer according to the present invention includes the seedling stage, and the number of applications of the topdressing fertilizer is preferably 4 to 6 times, more preferably 4 times. The first topdressing fertilizer is applied for the first topdressing in the seedling stage, and the second topdressing fertilizer is applied for the remaining topdressing. The time interval between the two applications of the second topdressing fertilizer can be 6 to 8 days, more preferably 7 days. As an optional embodiment, the salt concentration of the saline-alkali environment is ≥0.16% and the pH is ≥10; in a specific embodiment of the present invention, the effect is verified with a salt concentration of 0.16%-0.25% and a pH value of 10.08-10.56 in saline-alkali soil. As an optional embodiment, the saline-alkali environment of the present invention includes saline-alkali soil and / or saline-alkali water. As an optional embodiment, the field application amount of the base fertilizer in the fertilizer of the present invention is 200-400 mL per 2-4 plants; the field application amount of the topdressing fertilizer in the fertilizer is 200-400 mL per 2-4 plants. In a specific embodiment of the present invention, the application amount for laboratory potted plants is 250 mL per 2-4 plants.

[0075] In order to further illustrate the present invention, the technical solution provided by the present invention is described in detail below with reference to the accompanying drawings and embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0076] Measurement indicators:

[0077] Morphological indicators: plant height (ruler measurement method), stem diameter (vernier caliper measurement method), dry weight (oven drying weighing method), fresh weight (in vitro weighing method), leaf area (coefficient method), and main root length, root area, and root volume were measured using a root scanner (root scanner method).

[0078] Nutrient indicators: The nitrogen content of plant nutrients was determined by semi-micro distillation method, and the phosphorus, potassium, calcium, magnesium, zinc and nitrogen contents of plant nutrients were determined by inductively coupled plasma emission spectrometry, referring to "Soil Agrochemical Analysis" by Bao Shidan, 2000, China Agricultural University Press.

[0079] Photosynthetic index: Determine the chlorophyll content, which is measured using a SPAD chlorophyll meter.

[0080] Cell membrane system stability: Soluble sugar, soluble protein, malondialdehyde, and hydrogen peroxide levels were measured using Solebo kits. Soluble sugar and soluble protein can prevent water loss in the body and maintain the stability of the cell membrane structure.

[0081] Damage to the cell membrane: The conductivity meter method measures the relative conductivity, which reflects the damage to the cell membrane.

[0082] The parameters of the fertilizer are as follows:

[0083] Inositol: Molecular formula: C6H 12 O6, white powder, odorless, easily soluble in water, purchased from Shandong Gushuo Biotechnology Co., Ltd., purity ≥98%.

[0084] Urea: total nitrogen mass fraction ≥ 46%, biuret mass fraction ≤ 0.9%, water mass fraction ≤ 0.5% and methylene diurea mass fraction ≤ 0.6%; purchased from Yunnan Yuntianhua Co., Ltd.

[0085] Ammonium nitrogen fertilizer (monoammonium phosphate): the mass fraction of total nutrients (N+P2O5) is ≥58%, the mass fraction of total nitrogen is ≥8%, the mass fraction of available phosphorus is ≥48%, and the percentage of water-soluble phosphorus in available phosphorus is ≥80%; purchased from Yunnan Yuntianhua Co., Ltd.

[0086] Potassium sulfate: K2SO4 ≥ 99%, chloride ≤ 0.03%, acidity or alkalinity soluble in water ≤ 0.1%, heavy metal ≤ 0.002%, mass fraction of water ≤ 1%; purchased from Yunnan Yuntianhua Co., Ltd.

[0087] Magnesium sulfate, copper sulfate pentahydrate, manganese sulfate monohydrate, boric acid and EDTA-sodium iron are all analytically pure chemical reagents, and the brand is Aladdin; magnesium sulfate pentahydrate, copper sulfate monohydrate, manganese sulfate, boric acid and EDTA-sodium iron are dissolved in water to prepare fertilizer; among them,

[0088] Magnesium sulfate heptahydrate is colorless or white crystals; water-soluble effective magnesium oxide (MgO) content: ≥12.5%;

[0089] Copper sulfate pentahydrate is a blue crystalline powder, CuSO4·5H2O≥99.0%.

[0090] The (MnSO4·H2O) content of manganese sulfate monohydrate is 99.0-101.0 (w / %);

[0091] Boric acid is white powdery crystals or triclinic flaky crystals (H3BO3) with a content of ≥99.5%

[0092] Sodium iron EDTA is a light yellowish crystalline powder, and the weight of the dry product accounts for ≥95.0% of the total weight.

[0093] Example 1: Effect of inositol concentration on salt tolerance of corn

[0094] Test materials: inositol, corn (variety Zhengdan 958).

[0095] Treatment 1: The corn seeds were disinfected with a hydrogen peroxide solution having a mass concentration of 0.1% for 10 minutes, rinsed, and soaked in water for 24 hours after rinsing. The seeds were germinated in an incubator at 25° C. to obtain germinated seeds.

[0096] Vermiculite and coconut coir were mixed in a volume ratio of 3:7 to create a mixed matrix. This matrix was then placed in pots, and uniformly germinating seeds were selected and sown in the mixed matrix. The plants were then placed in a greenhouse at 25°C for cultivation. After sowing, 250 mL of neutral Hoagland nutrient solution were irrigated every three days. 200 mL of a 0.1 g / L inositol solution was irrigated at the roots 5, 10, 15, 20, and 25 days after sowing. When the third leaf was fully expanded, healthy plants with uniform growth were selected for salt stress treatment with 250 mL of a 150 mmol / L sodium chloride solution.

[0097] Treatment 2: Same as treatment 1, except that 250 mL of 0.2 g / L inositol solution was irrigated at the roots 5, 10, 15, 20, and 25 days after seed sowing.

[0098] Treatment 3: Same as treatment 1, except that 250 mL of 0.4 g / L inositol solution was irrigated at the roots 5, 10, 15, 20, and 25 days after seed sowing.

[0099] Treatment 4: Same as treatment 1, except that 250 mL of 0.8 g / L inositol solution was irrigated at the roots 5, 10, 15, 20, and 25 days after seed sowing.

[0100] Treatment 5: Same as treatment 1, except that 250 mL of 1.0 g / L inositol solution was irrigated at the roots 5, 10, 15, 20, and 25 days after seed sowing.

[0101] Treatment 6: Same as treatment 1, except that 250 mL of 1.2 g / L inositol solution was irrigated at the roots 5, 10, 15, 20, and 25 days after seed sowing.

[0102] Treatment 7 (CK): Same as treatment 1, except that 250 mL of clean water was irrigated at the roots 5, 10, 15, 20, and 25 days after seed sowing.

[0103] Each treatment was repeated four times. From Treatment 1 to Treatment 7, corn seedlings were harvested 30 days after sowing and tested.

[0104] The measured parameters were plant height, stem diameter, aboveground dry weight, aboveground fresh weight, chlorophyll (SPAD), leaf area, total root length, root surface area, and root volume. The results are shown in Tables 1 and Figures 1 to 5 , Figure 6 This is the phenotype diagram of corn seedlings.

[0105] Table 1 Results of index determination in different treatment groups

[0106] deal with Fresh root weight (g) Root dry mass (g) Total root length (cm) <![CDATA[Root surface area (cm 2 )]]> <![CDATA[Root volume (cm 3 )]]> CK (processing 7) 1.15±0.002b 0.13±0.003c 307.52±4.82d 76.75±2.03c 2.80±0.03a 0.1g / L (treatment 1) 1.16±0.004b 0.13±0.003c 329.54±6.03c 71.93±1.53d 2.71±0.05a 0.2g / L (treatment 2) 1.24±0.004a 0.19±0.005ab 373.26±5.73a 80.13±1.29ab 2.68±0.06a 0.4g / L (treatment 3) 1.23±0.004a 0.17±0.002b 359.55±5.16b 83.50±0.93a 2.59±0.01a 0.8g / L (treatment 4) 1.23±0.004a 0.20±0.004a 375.35±4.28a 85.64±1.56a 2.98±0.07a 1.0g / L (treatment 5) 1.04±0.004bc 0.14±0.001c 332.09±5.34c 73.27±2.53d 2.62±0.02a 1.2 g / L (treatment 6) 0.94±0.004c 0.16±0.002b 315.94±5.78d 72.70±1.62d 2.44±0.03a

[0107] It can be seen from the appearance phenotype and growth indicators that the inositol concentration of 0.4g / L to 0.8g / L has the best effect on alleviating salt damage to corn.

[0108] Example 2 Investigating the Effect of Inositol Dosage and Application Period on Salt Tolerance of Corn

[0109] Test materials: inositol, corn (variety: Suyuzhengdan 958).

[0110] Treatment 1 (T1): The corn seeds were sterilized with a 0.1% mass concentration hydrogen peroxide solution for 10 minutes, rinsed, and then soaked in water for 24 hours. The seeds were germinated in an incubator at 25° C. to obtain germinated seeds.

[0111] Vermiculite and coconut coir were mixed in a volume ratio of 3:7 to obtain a mixed matrix, which was placed in a flower pot. Seeds with consistent growth after germination were selected and sown in the mixed matrix of the flower pot for pot culture. The seeds were then placed in a greenhouse at 25°C for culture. 250 mL of a 0.4 g / L inositol solution was irrigated at the roots 5 days after sowing. 250 mL of a neutral Hoagland nutrient solution was irrigated every 3 days after sowing. When the third leaf was fully expanded, healthy plants with consistent growth were selected for salt stress treatment of corn by irrigating with 250 mL of a 150 mmol / L sodium chloride solution.

[0112] Treatment 2 (T2): Same as treatment 1, except that 500 mL of 0.4 g / L inositol solution was irrigated at the roots 5 days after seed sowing.

[0113] Treatment 3 (T3): Same as treatment 1, except that 750 mL of 0.4 g / L inositol solution was irrigated at the roots 5 days after seed sowing.

[0114] Treatment 4 (T4): Same as treatment 1, except that 250 mL of 0.4 g / L inositol solution was irrigated to the roots 5 days after seed sowing and 250 mL of 0.4 g / L inositol solution was irrigated to the roots 20 days after seed sowing.

[0115] Treatment 5 (T5): Same as treatment 4, except that 500 mL of 0.4 g / L inositol solution was irrigated to the roots 5 days after sowing and 500 mL of 0.4 g / L inositol solution was irrigated to the roots 20 days after sowing.

[0116] Treatment 6 (T6): Same as treatment 4, except that 750 mL of 0.4 g / L inositol solution was irrigated to the roots 5 days after seed sowing, and 750 mL of 0.4 g / L inositol solution was irrigated to the roots 20 days after seed sowing.

[0117] Treatment 7 (T7): Same as treatment 1, except that 250 mL of 0.4 g / L inositol solution was irrigated to the roots 5 days after seed sowing, 250 mL of 0.4 g / L inositol solution was irrigated to the roots 20 days after seed sowing, and 250 mL of 0.4 g / L inositol solution was irrigated to the roots 25 days after seed sowing.

[0118] Treatment 8 (T8): Same as treatment 7, except that 500 mL of 0.4 g / L inositol solution was irrigated to the roots 5 days after seed sowing, 500 mL of 0.4 g / L inositol solution was irrigated to the roots 20 days after seed sowing, and 500 mL of 0.4 g / L inositol solution was irrigated to the roots 25 days after seed sowing.

[0119] Treatment 9 (T9): Same as treatment 7, except that 750 mL of 0.4 g / L inositol solution was irrigated to the roots 5 days after seed sowing, 750 mL of 0.4 g / L inositol solution was irrigated to the roots 20 days after seed sowing, and 750 mL of 0.4 g / L inositol solution was irrigated to the roots 25 days after seed sowing.

[0120] Treatments 1 to 9 were set up with 3 replicates for each treatment. After 30 days, corn seedlings were harvested and the following measurements were made: plant height, stem diameter, aboveground dry weight, underground dry weight, and chlorophyll (SPAD). See Tables 2 to 3 and Figures 10-11 .

[0121] It can be seen that the appropriate dosage of inositol can alleviate corn salt damage, and excessive application will also cause harm to corn. Through experiments and cost considerations, the application effect of inositol at 250mL in the seedling stage (5 days after sowing) is the best.

[0122] Table 2 Test results of different indicators of corn in treatments 1 to 9

[0123]

[0124]

[0125] Note: T1-1, T1-2 and T1-3 are three parallel experiments of T1, T2-1, T2-2 and T2-3 are three parallel experiments of T2, and the rest T3-1, T3-2, T3-3, T4-1, T4-2, T4-3, T5-1, T5-2, T5-3, T6-1, T6-2, T6-3, T7-1, T7-2, T7-3, T8-1, T8-2, T8-3, T9-1, T9-2, T9-3 are also parallel experiments of the corresponding treatments.

[0126] Table 3 Test results of different indicators of corn in treatments 1 to 9

[0127]

[0128] Comparative Example 1: Exploring the effects of inositol on corn under non-salt-alkali stress

[0129] Test materials: inositol, corn (variety Zhengdan 958).

[0130] Treatment 1: The corn seeds were disinfected with a hydrogen peroxide solution having a mass concentration of 0.1% for 10 minutes, rinsed, and soaked in water for 24 hours after rinsing. The seeds were germinated in an incubator at 25° C. to obtain germinated seeds.

[0131] Vermiculite and coconut coir were mixed in a volume ratio of 3:7 to obtain a mixed matrix, which was placed in flower pots. Seeds with consistent growth after germination were selected and sown in the mixed matrix in the flower pots. The seeds were placed in a greenhouse at 25°C for potted culture. 200 mL of 0.1 g / L inositol solution was irrigated at the roots 5, 10, 15, 20, and 25 days after sowing. When the seeds reached the two-leaf and one-heart stage, Hoagland nutrient solution was used for culture. When the third leaf was fully expanded, healthy plants with consistent growth were selected for testing.

[0132] Treatment 2: Same as treatment 1, except that 200 mL of 0.2 g / L inositol solution was irrigated at the roots 5, 10, 15, 20, and 25 days after seed sowing.

[0133] Treatment 3: Same as treatment 1, except that 200 mL of 0.4 g / L inositol solution was irrigated at the roots 5, 10, 15, 20, and 25 days after seed sowing.

[0134] Treatment 4: Same as treatment 1, except that 200 mL of 0.8 g / L inositol solution was irrigated at the roots 5, 10, 15, 20, and 25 days after seed sowing.

[0135] Treatment 5: Same as treatment 1, except that 200 mL of 1.0 g / L inositol solution was irrigated at the roots 5, 10, 15, 20, and 25 days after seed sowing.

[0136] Treatment 6: Same as treatment 1, except that 200 mL of 1.2 g / L inositol solution was irrigated at the roots 5, 10, 15, 20, and 25 days after seed sowing.

[0137] Treatment 7 (CK): Same as treatment 1, except that 200 mL of clean water was irrigated at the roots 5, 10, 15, 20, and 25 days after seed sowing.

[0138] There are 7 treatments in total, 1 to 7, and 4 replicates for each treatment. The corn seedlings were harvested 30 days after sowing. The plant height, stem diameter, aboveground fresh weight, underground fresh weight, chlorophyll (SPAD), aboveground dry weight, and underground dry weight were measured. Figures 7 to 9 It can be seen that inositol does not promote the growth of corn plants under non-salt stress conditions, but instead has a negative regulatory effect. Therefore, the application of inositol should only be effective under salt stress.

[0139] Example 3

[0140] Objective: To compare the salt tolerance effects of inositol and methyl jasmonate and to explore whether there is a synergistic effect between the two

[0141] Experimental materials: Zhengdan 958, water-soluble fertilizer, inositol, methyl jasmonate, moderate saline-alkali soil in Inner Mongolia, and high saline-alkali soil in Inner Mongolia. The saline-alkali soil parameters are shown in Table 4.

[0142] Table 4 Parameters of different types of saline-alkali soil

[0143]

[0144] Test content:

[0145] Formula of water-soluble fertilizer:

[0146] Each 250 mL of water-soluble fertilizer contains 0.344 g of monoammonium phosphate, 1.54 g of urea, 0.23 g of potassium sulfate, 0.0195 g of magnesium sulfate, 0.00275 g of sodium ferric EDTA, 0.0035 g of manganese sulfate monohydrate, 0.00034 g of boric acid, and 0.001 g of copper sulfate pentahydrate. The water-soluble fertilizer is prepared by dissolving the aforementioned fertilizers in deionized water as the solvent. This water-soluble fertilizer is designated Solution 1. The pH of Solution 1 is approximately 4 to 4.5.

[0147] Each 250 mL of solution 2 contains 0.344 g of monoammonium phosphate and 0.1 g of inositol. The pH value of solution 2 is approximately 4.5.

[0148] Each 250 mL of solution 3 contained 0.344 g of monoammonium phosphate and 0.025 μmol of methyl jasmonate. The pH value of solution 3 was approximately 4.5.

[0149] Each 250 mL of solution 4 contained 0.344 g of monoammonium phosphate, 0.025 μmol of methyl jasmonate, and 0.1 g of inositol. The pH value of solution 4 was approximately 4.5.

[0150] Each 250 mL of water-soluble fertilizer contains 0.344 g of monoammonium phosphate, 0.1 g of inositol, 1.54 g of urea, 0.23 g of potassium sulfate, 0.0195 g of magnesium sulfate, 0.00275 g of sodium ferric EDTA, 0.0035 g of manganese sulfate monohydrate, 0.00034 g of boric acid, and 0.001 g of copper sulfate pentahydrate. The water-soluble fertilizer is prepared by dissolving the aforementioned fertilizers in deionized water as the solvent. This water-soluble fertilizer is designated Solution 5. The pH of Solution 5 is approximately 4 to 4.5.

[0151] Each 250 mL of water-soluble fertilizer contains 0.344 g of monoammonium phosphate, 0.025 μmol of methyl jasmonate, 1.54 g of urea, 0.23 g of potassium sulfate, 0.0195 g of magnesium sulfate, 0.00275 g of sodium ferric EDTA, 0.0035 g of manganese sulfate monohydrate, 0.00034 g of boric acid, and 0.001 g of copper sulfate pentahydrate. The water-soluble fertilizer is prepared by dissolving the aforementioned fertilizers in deionized water using deionized water as the solvent. This water-soluble fertilizer is designated Solution 6. The pH of Solution 6 is approximately 4 to 4.5.

[0152] Each 250 mL of the water-soluble fertilizer contains 0.344 g of monoammonium phosphate, 0.025 μmol of methyl jasmonate, 0.1 g of inositol, 1.54 g of urea, 0.23 g of potassium sulfate, 0.0195 g of magnesium sulfate, 0.00275 g of sodium ferric EDTA, 0.0035 g of manganese sulfate monohydrate, 0.00034 g of boric acid, and 0.001 g of copper sulfate pentahydrate. The water-soluble fertilizer is prepared using deionized water as the solvent. The aforementioned fertilizers are dissolved in deionized water to obtain the water-soluble fertilizer. This water-soluble fertilizer is designated as Solution 7. The pH of Solution 7 is approximately 4 to 4.5.

[0153] Treatment 1 (Low-T1): Corn seeds were sterilized with 0.1% hydrogen peroxide for 10 minutes, rinsed, and then soaked in water for 24 hours. Germination was then accelerated in a 25°C incubator. Seeds with consistent growth were selected for sowing in the medium-to-low saline-alkali soil of Inner Mongolia. Three to four seeds were sown per pot. After sowing, 250 mL of Solution 2 was drip-applied at the sowing stage. After seedling emergence, the first topdressing was performed with 250 mL of Solution 5. Thereafter, topdressing was performed every 7 days with 250 mL of Solution 1 each time. Harvest the corn seedlings 30 days after sowing.

[0154] Treatment 2 (Low-T2): Same as Treatment 1, except that 250 mL of Solution 3 was drip-applied after sowing. The first topdressing was performed after seedling emergence, using 250 mL of Solution 6. Thereafter, topdressing was performed every 7 days, using 250 mL of Solution 1. Methyl jasmonate was applied in the first topdressing after seedling emergence at a concentration of 0.1 μmol / L in a dosage of 250 mL. Corn seedlings were harvested 30 days after sowing.

[0155] Treatment 3 (Low-T3): Same as Treatment 1, except that 250 mL of Solution 4 was drip-applied after sowing. The first topdressing was performed after seedling emergence, using 250 mL of Solution 7. Thereafter, topdressing was performed every 7 days, using 250 mL of Solution 1 each time. Corn seedlings were harvested 30 days after sowing.

[0156] Control (Low-CK): Same as Treatment 1, except that 250 mL of monoammonium phosphate solution (containing 0.344 g of monoammonium phosphate) was drip-applied after sowing. 250 mL of Solution 1 was applied every 7 days after seedling emergence. Harvest corn seedlings 30 days after sowing.

[0157] Treatment 4 (High-T1): Same as treatment 1, except that the sowing soil was high saline-alkali soil in Inner Mongolia.

[0158] Treatment 5 (High-T2): Same as treatment 2, except that the sowing soil was high saline-alkali soil in Inner Mongolia.

[0159] Treatment 6 (High-T3): Same as treatment 3, the only difference being that the sowing soil was the high saline-alkali soil of Inner Mongolia.

[0160] Control group (high-CK): Same as the control group (low-CK), the only difference being that the sowing soil was the high saline-alkali soil of Inner Mongolia.

[0161] Treatments 1 to 6, CK1, and CK2 were all conducted in the modern greenhouse of Yuntianhua. Four replicates were set up for each treatment. 3 to 6 plants were measured in each replicate. The results are shown in Tables 5 to 11 and Figures 12-18 .

[0162] Table 5 Growth index measurement results of corn in treatments 1 to 6, CK1, and CK2

[0163]

[0164]

[0165] Note: CK, T1, T2 and T3 of moderate saline-alkali soil represent low-CK, low-T1, low-T2 and low-T3 respectively; CK, T1, T2 and T3 of high saline-alkali soil represent high-CK, high-T1, high-T2 and high-T3 respectively; the same below.

[0166] Table 6 Growth index measurement results of corn in treatments 1 to 6, CK1, and CK2

[0167]

[0168] Table 7 Nutrient index measurement results of corn in treatments 1 to 6, CK1, and CK2

[0169]

[0170] Table 8 Nutrient index measurement results of corn in treatments 1 to 6, CK1, and CK2

[0171]

[0172] Table 9 Physiological index measurement results of corn in treatments 1 to 6, CK1, and CK2

[0173]

[0174] Table 10 Physiological index measurement results of corn in treatments 1 to 6, CK1, and CK2

[0175]

[0176] It can be seen that the growth indicators: plant height and stem diameter of the inositol-treated group were better than those of the control group, and there was little difference in the effects of inositol and methyl jasmonate on the phenotypes and biomass of the aboveground and underground parts.

[0177] Table 11 Root growth of corn seedlings in different types of saline-alkali soil

[0178]

[0179] Nutrient indicators: The inositol treatment group improved the corn seedlings' absorption of nitrogen, phosphorus, calcium, and zinc. Physiological indicators: The inositol treatment group protected the corn seedlings' cell membranes, increased the production of osmotic regulating substances, and improved the corn seedlings' stress resistance.

[0180] Example 4 Verification of the synergistic effect of inositol and ammonium nitrogen fertilizer

[0181] The pH of saline-alkali soil is 9.34-9.56, and the water-soluble salt content is between 0.15%. It is a coastal saline-alkali soil and belongs to moderate saline-alkali soil.

[0182] Test content:

[0183] T1, Inositol + Monoammonium Phosphate: Disinfect corn seeds, soak them in water, and place them in a 28°C incubator to accelerate germination. Select uniformly germinated seeds for sowing. Fill each pot with 6 kg of saline-alkali soil and sow 3-4 germinated corn seeds per pot. Thin the seedlings to 2 seeds after emergence. After sowing, drip-feed 250 mL of Fertilizer Solution 1. 250 mL of Fertilizer Solution 1 contains 0.344 g of monoammonium phosphate and 0.1 g of Inositol. The pH of Solution 1 is approximately 4.5.

[0184] Start dripping 250 mL of Fertilizer Solution 2 once after seedlings emerge. Then drip 250 mL of Fertilizer Solution 3 every 7 days for a total of 4 times. Harvest corn seedlings around the 35th day after sowing.

[0185] Each 250 mL of fertilizer solution 2 contains 0.344 g of monoammonium phosphate, 0.1 g of inositol, 1.54 g of urea, 0.23 g of potassium sulfate, 0.0195 g of magnesium sulfate, 0.00275 g of sodium ferric EDTA, 0.0035 g of manganese sulfate monohydrate, 0.00034 g of boric acid, and 0.001 g of copper sulfate pentahydrate. The pH of solution 2 is approximately 4 to 4.5.

[0186] Each 250 mL of fertilizer solution 3 contains 0.344 g of monoammonium phosphate, 1.54 g of urea, 0.23 g of potassium sulfate, 0.0195 g of magnesium sulfate, 0.00275 g of sodium ferric EDTA, 0.0035 g of manganese sulfate monohydrate, 0.00034 g of boric acid, and 0.001 g of copper sulfate pentahydrate. The pH of solution 3 is approximately 4 to 4.5.

[0187] T2: Same as T1, the only difference is that 250 mL of fertilizer solution 4 is drip-applied after sowing, and 250 mL of fertilizer solution 5 is drip-applied from the beginning of seedling emergence, with solution 5 drip-applied once; thereafter, fertilizer solution 6 is drip-applied every 7 days, for a total of 4 times, with each application amount of 250 mL.

[0188] The formula of fertilizer solution 4 is as follows: each 250 mL of fertilizer solution 4 contains 1.73 g of potassium dihydrogen phosphate, 0.077 g of urea, and 0.1 g of inositol. The pH value of solution 4 is 4.7.

[0189] The formula of Fertilizer Solution 5 is as follows: per 250 mL, Fertilizer Solution 5 contains 0.335 g of potassium dihydrogen phosphate, 0.1 g of inositol, 0.193 g of potassium sulfate, 1.34 g of urea, 0.0195 g of magnesium sulfate, 0.00275 g of sodium ferric EDTA, 0.0035 g of manganese sulfate monohydrate, 0.00034 g of boric acid, and 0.001 g of copper sulfate pentahydrate. The pH of Solution 5 is approximately 4.5 to 4.8.

[0190] The formula of Fertilizer Solution 6 is as follows: per 250 mL of Fertilizer Solution 6, it contains 0.335 g of potassium dihydrogen phosphate, 0.193 g of potassium sulfate, 1.34 g of urea, 0.0195 g of magnesium sulfate, 0.00275 g of sodium ferric EDTA, 0.0035 g of manganese sulfate monohydrate, 0.00034 g of boric acid, and 0.001 g of copper sulfate pentahydrate. The pH of Solution 6 is approximately 4.5 to 4.8.

[0191] T3: Same as T1, except that 250 mL of monoammonium phosphate solution (MAP) containing 0.344 g MAP was drip-applied after sowing. Fertilizer Solution 3 was drip-applied starting after seedling emergence and every 7 days thereafter. The composition of Fertilizer Solution 3 was the same as T1. Fertilizer Solution 3 was drip-applied four times, with 250 mL applied each time.

[0192] CK: Same as T1, except that 250 mL of Fertilizer Solution 7 was drip-applied after sowing. The composition of Fertilizer Solution 7 was 1.73 g potassium dihydrogen phosphate and 0.077 g urea per 250 mL of Fertilizer Solution 7 to ensure consistent initial phosphorus and nitrogen nutrient levels with those in the above treatments. Fertilizer Solution 6 was drip-applied starting after seedling emergence. The composition of Fertilizer Solution 6 was the same as in T2. ​​The first drip application of Fertilizer Solution 6 was after emergence, followed by a 250 mL application every 7 days for a total of four applications. The pH of Solution 7 was approximately 4.7.

[0193] Experimental treatment: The experiment was conducted in Yuntianhua Modern Greenhouse. There were 4 treatment groups and 4 replicates for each treatment. Figures 19-21 and Tables 12-13.

[0194] Growth indicators (plant height, stem diameter, leaf area, chlorophyll (SPAD), aboveground dry weight, underground dry weight, see Figure 19): Inositol + monoammonium phosphate significantly promoted the biomass of corn plant height, stem diameter, leaf area, chlorophyll (SPAD), aboveground dry weight, and underground dry weight under saline-alkali stress, achieving the effect of alleviating salt damage. The growth of corn seedlings potted on the 35th day under different treatments in Example 4 is shown in FIG. Figure 22 .

[0195] Table 12 Growth index and nutrient content of corn under different treatments

[0196]

[0197]

[0198] Table 13 Nutrient content determination results of corn with different treatments

[0199]

[0200] Physiological indicators (malondialdehyde content, hydrogen peroxide content, soluble protein content, soluble sugar content see Figure 20 ): The combined application of inositol and monoammonium phosphate can alleviate the damage caused by salt and alkali to corn, reduce the content of malondialdehyde and hydrogen peroxide, and protect the cell membrane; at the same time, it maintains the cell osmotic balance by accumulating the content of osmotic regulating substances such as soluble protein and soluble sugar. Nutrient indicators (nitrogen, phosphorus, potassium, zinc, calcium, and magnesium content in the aboveground part are shown in Figure 2). Figure 21 ): The combined application of inositol and monoammonium phosphate promoted the absorption of nitrogen, phosphorus, calcium and zinc nutrients in saline-alkali soil corn, while the absorption and utilization of potassium and magnesium showed little difference.

[0201] Example 5: Exploring the Effect of Inositol on the Physicochemical Properties of Water-Soluble Fertilizers

[0202] The dissolution of 0.1 g to 0.5 g of inositol was added to the water-soluble fertilizer prepared in Example 3 in 5 mL. The test tubes from left to right contained no inositol, 0.1 g inositol, 0.2 g inositol, 0.3 g inositol, 0.4 g inositol and 0.5 g inositol. The dissolution was observed after 2 h of addition. The results are shown in FIG. Figure 23 It can be seen that inositol and water-soluble fertilizers will not precipitate.

[0203] In summary, under saline-alkali stress, sodium ions enter the plant body and inhibit plant growth. Ammonium nitrogen fertilizer can improve the salt-alkali resistance of inositol. The fertilizer of the present invention is a functional water-soluble fertilizer product containing inositol that is salt-resistant and growth-promoting. It can promote plant growth in saline-alkali soil, promote plant nutrient absorption, and improve plant salt-alkali resistance.

[0204] Although the above embodiment provides a detailed description of the present invention, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the scope of protection of the present invention.

Claims

1. Application of ammonium nitrogen fertilizer in improving the salt-alkali resistance of inositol.

2. The application according to claim 1, characterized in that The ammonium nitrogen fertilizer includes monoammonium phosphate.

3. A base fertilizer, characterized in that: The base fertilizer comprises ammonium nitrogen fertilizer and inositol; the base fertilizer contains 1.2-1.6 g / L of ammonium nitrogen fertilizer and 0.4-0.8 g / L of inositol; and the pH value of the base fertilizer is 4-4.

5.

4. A fertilizer, characterized in that Comprising the independently packaged base fertilizer according to claim 3 and independently packaged topdressing fertilizer; The topdressing fertilizer comprises a first topdressing fertilizer and a second topdressing fertilizer which are separately packaged; the first topdressing fertilizer contains 0.8-1.0 g / L of potassium fertilizer, 1.2-1.6 g / L of ammonium nitrogen fertilizer, 0.4-0.8 g / L of inositol and 5.5-6.5 g / L of amide nitrogen fertilizer; The second topdressing fertilizer contains 0.8-1.0 g / L of potassium fertilizer, 1.2-1.6 g / L of ammonium nitrogen fertilizer and 5.5-6.5 g / L of amide nitrogen fertilizer; The amide nitrogen fertilizer includes urea.

5. The fertilizer according to claim 4, characterized in that The topdressing fertilizer also contains macroelement fertilizer and trace element fertilizer; the macroelement fertilizer includes 0.0195-0.078 g / L magnesium fertilizer; the trace element fertilizer includes one or more of 0.004-0.016 g / L copper fertilizer, 0.0035-0.014 g / L manganese fertilizer, 0.00034-0.00136 g / L boron fertilizer and 0.00275-0.011 g / L iron fertilizer.

6. The fertilizer according to claim 5, characterized in that The magnesium fertilizer includes magnesium sulfate, the copper fertilizer includes copper sulfate pentahydrate, the manganese fertilizer includes manganese sulfate monohydrate, the boron fertilizer includes boric acid, and the iron fertilizer includes EDTA-sodium iron.

7. Use of the base fertilizer according to claim 3 or the fertilizer according to any one of claims 4 to 6 in one or more of the following 1) to 3), 1) Plant growth promotion in saline-alkali environment; 2) Promote plant nutrient absorption in saline-alkali environments; 3) Improve the salt resistance of plants in saline-alkali environments.

8. A method for using the base fertilizer according to claim 3 or the fertilizer according to any one of claims 4 to 6, characterized in that: include: The base fertilizer or the fertilizer is applied to plants grown in a saline-alkali environment.

9. The method of use according to claim 8, characterized in that: The plant includes corn; the application period of the base fertilizer in the fertilizer includes the sowing period; and the application period of the topdressing fertilizer in the fertilizer includes the seedling stage.

10. The method of use according to claim 8, characterized in that: The field application amount of the base fertilizer in the fertilizer is 200-400 mL per 2-4 plants; the field application amount of the topdressing fertilizer in the fertilizer is 200-400 mL per 2-4 plants.

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