High-efficiency CTC detection integrated equipment

Abstract

The invention discloses high-efficiency CTC detection integrated equipment, which comprises a base and a dyeing assembly arranged on the base, the dyeing assembly is used for dyeing target cells, and the high-efficiency CTC detection integrated equipment further comprises a fixing frame arranged on the base; the turnover mechanism is connected to the fixing frame in a reciprocating rotation mode; the liquid suction pipe is connected to the turnover mechanism, and the turnover mechanism drives the liquid suction pipe to be inserted into and pulled out of the dyeing assembly when rotating in a reciprocating mode; the waste liquid negative pressure pump is connected with the liquid suction assembly through a pipeline and used for providing negative pressure and sucking waste liquid in the dyeing assembly. According to the CTC detection device, the liquid suction pipe is driven by the turnover mechanism to be automatically inserted into or extracted from the dyeing assembly, and the waste liquid is automatically sucked by utilizing the negative pressure pump, so that the problems of low manual operation efficiency and high biological safety risk are effectively solved, and high efficiency and safety of a CTC detection process are realized.

Description

Technical Field

[0001] The present invention belongs to the field of biomedical devices, and in particular, relates to a high-efficiency CTC detection integrated device. Background Art

[0002] The pharmaceutical industry and the biomedical engineering industry are the two major pillars of the modern pharmaceutical industry. The biomedical industry is composed of the biotechnology industry and the pharmaceutical industry. Biomedical engineering is the comprehensive application of the principles and methods of life science and engineering science. It understands the structure, function and other life phenomena of the human body at multiple levels of molecules, cells, tissues, organs and even the entire human body system from an engineering perspective. It is a general term for studying artificial materials, products, devices and system technologies used for disease prevention, treatment, human function assistance and health care.

[0003] In recent years, some new tumor diagnostic detection technologies have emerged in the field of biomedical engineering, such as the circulating tumor cell (CTCs) detection method known as liquid biopsy. CTC detection is easy to obtain samples, which overcomes the shortcomings of histopathological examination, which is inconvenient to obtain samples and has certain damage to patients. According to research, CTCs can be found in peripheral blood before solid tumors form, so CTC detection is very suitable for early screening and early diagnosis of malignant tumors. CTC detection has a good effect on the prognosis of malignant tumors, disease progression monitoring, recurrence prediction, monitoring of small lesions after malignant tumor surgery, and the design of targeted drug therapy and monitoring of treatment effects. It is currently an advanced method for early screening and diagnosis of malignant tumors. Since the content of CTCs in peripheral blood is very low, CTC detection requires enrichment of CTCs before detection.

[0004] Currently, the staining step in the CTC detection process requires multiple processing of body fluid samples, and operators need to manually remove the waste liquid after staining. Especially when facing high-throughput samples, the detection efficiency will be significantly reduced. In addition, direct contact with waste liquid containing tumor cells will increase the operator's exposure risk.

[0005] Therefore, there is an urgent need to develop an integrated and automated waste liquid treatment component to improve the overall efficiency and reliability of CTC detection equipment. Summary of the Invention

[0006] In order to solve at least some of the above problems, the first object of the present invention is to provide a high-efficiency CTC detection integrated device that can improve detection efficiency.

[0007] In order to solve the above technical problems, the basic concept of the technical solution adopted by the present invention is:

[0008] A high-efficiency CTC detection integrated device includes a base and a staining component disposed on the base, the staining component being used to stain target cells, and further comprising:

[0009] A fixing frame is arranged on the base;

[0010] The turning mechanism is connected to the fixed frame in a reciprocating and rotatable manner;

[0011] A pipette is connected to the flip mechanism, which drives the pipette to be inserted into and removed from the dyeing assembly during reciprocating rotation;

[0012] The waste liquid negative pressure pump is connected to the liquid suction component through a pipeline, and is used to provide negative pressure and suck the waste liquid in the dyeing component.

[0013] In some embodiments, the flipping mechanism comprises:

[0014] a rotating frame, wherein the pipette is arranged on the rotating frame;

[0015] The driving motor is connected to the fixed frame and is used to drive the rotating frame to rotate back and forth along its own axis between a first position and a second position. During the process of the rotating frame rotating from the first position to the second position, the pipette is inserted into the dyeing component and gradually withdrawn from the dyeing component.

[0016] In some embodiments, the pipette extends radially along the rotating frame, and a plurality of pipettes are arranged on the rotating frame along the axial direction.

[0017] In some embodiments, the pipette is mounted above or diagonally above the staining component.

[0018] In some embodiments, the staining component comprises:

[0019] dyeing tank;

[0020] The collecting tank is arranged at one side of the dyeing tank and is communicated with the dyeing tank. Both the collecting tank and the dyeing tank have upward notches. The pipette is inserted into or withdrawn from the collecting tank through the notches of the collecting tank.

[0021] Furthermore, a U-shaped opening is formed at the connection between the collecting tank and the dyeing tank, and the U-shaped opening is used to avoid the pipette.

[0022] In some embodiments, the bottom wall of the collecting trough transitions obliquely to the dyeing trough, and the bottom wall of the collecting trough is lower than the bottom wall of the dyeing trough.

[0023] Furthermore, the bottom wall of the dyeing tank is provided with a tray protruding from the bottom wall and used to hold the filter membrane. The tray and the bottom wall and side walls of the dyeing tank form a diversion channel for the waste liquid to flow to the collection tank.

[0024] In some embodiments, a plurality of dyeing assemblies are arranged along the axial direction of the rotating frame, and the collecting tank and the dyeing tank of each dyeing assembly are arranged along the radial direction of the rotating frame.

[0025] Furthermore, it also includes:

[0026] An enrichment component is provided on the base and is used for enriching target cells;

[0027] The liquid adding assembly includes a first guide rail arranged on the base and a first liquid adding tube and a second liquid adding tube slidingly matched with the first guide rail. The first liquid adding tube is used to add a first reagent to the staining assembly, and the second liquid adding tube is used to add a second reagent to the enrichment assembly.

[0028] After adopting the above technical solution, the present invention has the following beneficial effects compared with the prior art.

[0029] The flipping mechanism of the present application can automatically complete the precise insertion and removal of the pipette into the staining component, and the waste liquid negative pressure pump can automatically perform the waste liquid suction action, replacing the multi-step operation of the operator manually removing the waste liquid in the traditional process. At the same time, this automated process can be repeated quickly and reliably, which is particularly suitable for high-throughput CTC detection scenarios that require processing a large number of samples, significantly shortening the sample processing cycle and effectively improving the overall detection efficiency.

[0030] Operators do not need to manually operate the pipette to touch or transfer the stained waste liquid containing tumor cells. The insertion, aspiration, and withdrawal of the pipette are all completed automatically by the equipment, avoiding direct contact, reducing the risk of operators being exposed to potential biological hazards, and significantly reducing operational risks.

[0031] The specific embodiments of the present invention are further described in detail below with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] The accompanying drawings are part of the present invention and are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention, but do not constitute an undue limitation of the present invention. Obviously, the drawings described below are only some embodiments. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without inventive effort. In the accompanying drawings:

[0033] Figure 1 This is a three-dimensional diagram of the rotating frame in the high-efficiency CTC detection integrated device provided by an embodiment of the present application when it is in a first orientation;

[0034] Figure 2 This is a three-dimensional diagram of the rotating frame in the high-efficiency CTC detection integrated device provided by an embodiment of the present application when it is in the second orientation;

[0035] Figure 3 Schematic diagram of the structure of a high-efficiency CTC detection integrated device provided in an embodiment of the present application;

[0036] Figure 4 yes Figure 3 Enlarged view of area A in the middle;

[0037] Figure 5 yes Figure 3 Left view of;

[0038] Figure 6 yes Figure 3 Top view of .

[0039] In the picture:

[0040] 1. Base;

[0041] 2. Fixed frame; 21. Pipette; 23. Rotating frame; 24. Driving motor;

[0042] 3. Dyeing tank; 31. Collection tank; 33. U-shaped opening; 34. Tray; 35. Diversion channel;

[0043] 4. Enrichment component; 41. Connecting seat; 42. Filter;

[0044] 5. Liquid adding assembly; 51. First guide rail; 52. First liquid adding pipe; 53. Second liquid adding pipe.

[0045] It should be noted that these drawings and textual descriptions are not intended to limit the conceptual scope of the present invention in any way, but rather to illustrate the concept of the present invention for those skilled in the art by referring to specific embodiments. DETAILED DESCRIPTION

[0046] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. The following embodiments are used to illustrate the present invention but are not used to limit the scope of the present invention.

[0047] In the description of the present invention, it should be noted that the terms "upper", "lower", "front", "back", "left", "right", "vertical", "inside", "outside", etc., indicating directions or positional relationships, are based on the directions or positional relationships shown in the accompanying drawings. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction. Therefore, they cannot be understood as limiting the present invention.

[0048] In the description of the present invention, it should be noted that, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "connected" should be understood broadly. For example, they may refer to fixed, detachable, or integral connections; mechanical or electrical connections; and direct or indirect connections through an intermediary. Those skilled in the art will understand the specific meanings of the above terms in the present invention based on the specific circumstances.

[0049] See Figures 1-6 The present application provides a high-efficiency CTC detection integrated device, including a base 1 and a staining component arranged on the base 1, the staining component is used to stain target cells, and also includes a fixing frame 2, a flipping mechanism, a pipette 21 and a waste liquid negative pressure pump (not shown in the figure).

[0050] Among them, the fixed frame 2 is set on the base 1, the flipping mechanism is connected to the fixed frame 2 in a reciprocating manner, the pipette 21 is connected to the flipping mechanism, and the flipping mechanism drives the pipette 21 to insert and withdraw from the dyeing component when reciprocating. The waste liquid negative pressure pump is connected to the pipette component through a pipeline, and is used to provide negative pressure and suck the waste liquid in the dyeing component.

[0051] The automatic waste liquid removal operation process of the high-efficiency CTC detection integrated device of this application is as follows:

[0052] Preparation stage: The body fluid sample containing target cells (such as CTCs) is combined with the filter membrane to complete the staining reaction in the staining component of the device.

[0053] Waste liquid suction start: When it is necessary to remove the waste liquid in the dyeing component, the operator starts the equipment.

[0054] Insertion of the pipette 21: The flipping mechanism starts to rotate under the drive. Since the pipette 21 is fixedly connected to the flipping mechanism, the rotational movement of the flipping mechanism drives the pipette 21 to rotate synchronously. During the rotation process, the end of the pipette 21 used for pipetting is inserted below the liquid surface of the waste liquid to be discharged in the dyeing component.

[0055] Negative pressure suction: The waste liquid negative pressure pump connected to the pipette 21 through the pipeline is started simultaneously or subsequently. The waste liquid negative pressure pump generates a stable negative pressure, which is transmitted to the pipette 21 through the pipeline. Under the action of negative pressure, the waste liquid in the dyeing component is continuously sucked out, discharged through the pipette 21 and the pipeline, and transported to the designated waste liquid collection container.

[0056] The pipette 21 is evacuated: After the waste liquid is sucked out, the waste liquid negative pressure pump stops working, and the flip mechanism rotates again to perform reverse movement. The rotation of the flip mechanism drives the pipette 21 to move and reset, so that its end is completely withdrawn from the inside of the dyeing component and returns to the initial or safe parking position.

[0057] The flipping mechanism of the present application can automatically complete the precise insertion and withdrawal of the pipette 21 into the staining component, and the waste liquid negative pressure pump can automatically perform the waste liquid suction action, replacing the multi-step operation of the operator manually removing the waste liquid in the traditional process. At the same time, the automated process can be repeated quickly and reliably, which is particularly suitable for high-throughput CTC detection scenarios that require processing a large number of samples. It significantly shortens the sample processing cycle and effectively improves the overall detection efficiency.

[0058] Furthermore, the operator does not need to manually operate the pipette to contact or remove the stained waste liquid containing tumor cells. The insertion, suction, and withdrawal of the pipette 21 are all automatically completed by the device, avoiding direct contact, reducing the risk of the operator being exposed to potential biological hazards, and significantly reducing operational risks.

[0059] In some embodiments, see Figures 1-6 The flipping mechanism includes a rotating frame 23 and a driving motor 24. The pipette 21 of the rotating frame 23 is set on the rotating frame 23, and its position and orientation change with the rotation of the rotating frame 23. The driving motor 24 is connected to the fixed frame 2 and is used to drive the rotating frame 23 to rotate back and forth along its own axis between the first orientation and the second orientation. During the process of the rotating frame 23 rotating from the first orientation to the second orientation, the pipette 21 is gradually withdrawn from the dyeing assembly by being inserted into the dyeing assembly.

[0060] See Figure 1 When the rotating frame 23 is in the first position, the end of the pipette 21 is below the liquid level of the waste liquid to be discharged in the dyeing component, that is, the pipette 21 is in an inserted state; when the rotating frame 23 rotates from the first position to the second position, the pipette 21 is synchronously driven to move upward, and its end is gradually withdrawn from the inside of the dyeing component. Figure 2 , and finally completely exit the dyeing component when reaching the second position, that is, the withdrawal state.

[0061] When it is necessary to suck out the waste liquid, the driving motor 24 first rotates the rotating frame 23 together with the pipette 21 to the first position, ensuring that the end of the pipette 21 is inserted below the liquid level of the waste liquid in the dyeing component. Subsequently or at the same time, the waste liquid negative pressure pump is started, and the waste liquid in the dyeing component is sucked out through the pipette 21 connected by the pipeline.

[0062] After the waste liquid is sucked out, the drive motor 24 drives the rotating frame 23 to rotate from the first position to the second position. During this rotation process, the pipette 21 is lifted smoothly and finally completely pulled out of the staining component. After the pipette 21 reaches the second position, it can be maintained in this position to avoid interfering with subsequent operations or sample replacement.

[0063] The present application realizes fully programmed automatic control of the flipping action through the setting of the drive motor 24, which cooperates with the start and stop of the waste liquid negative pressure pump to further reduce the manual intervention link. The drive motor 24 directly drives the rotating frame 23 for precise angle control. The structure is simple and compact and can be easily integrated into the equipment frame composed of the fixed frame 2 and the base 1.

[0064] In addition, the design of fixing the pipette 21 to the rotating frame 23 ensures that its trajectory during the flipping motion is stable and repeatable, and the position of each insertion and withdrawal is highly consistent, avoiding the error or collision risk that may be caused by manual operation or complex linear motion mechanisms. When the pipette 21 is in the second position, it is away from the working area of ​​the staining component, providing the operator with a safer and more spacious operating space for changing samples or performing other steps, further reducing the risk of accidental contact.

[0065] In some embodiments, see Figures 1-6 The pipette 21 extends along the radial direction of the rotating frame 23 , and a plurality of pipettes 21 are arranged on the rotating frame 23 along the axial direction.

[0066] Furthermore, each pipette 21 is installed extending along the radial direction of the rotating frame 23. This layout allows all pipettes 21 to move synchronously when the rotating frame 23 rotates. When the driving motor 24 drives the rotating frame 23 to rotate to the first position, multiple pipettes 21 are simultaneously inserted into their corresponding dyeing components. Similarly, when the rotating frame 23 rotates from the first position to the second position, multiple pipettes 21 are synchronously and gradually pulled out of their corresponding dyeing components.

[0067] Each of the multiple pipettes 21 is connected to a waste liquid negative pressure pump via its own pipeline. When the turret 23 is in the first position, the waste liquid negative pressure pump is activated, and the negative pressure provided by the waste liquid negative pressure pump simultaneously acts on each of the pipettes 21, thereby simultaneously aspirating waste liquid from multiple staining components. The pipettes 21 are controlled by a common turning mechanism and waste liquid negative pressure pump, ensuring highly consistent and repeatable waste liquid aspiration across all channels. This helps ensure uniform processing conditions across different samples and enhances the reliability of test results.

[0068] After starting the waste liquid negative pressure pump, the waste liquid in multiple staining components is sucked out in parallel, reducing the sequential operation time required for traditional manual or single-channel automatic processing methods, greatly improving the waste liquid removal efficiency and sample throughput, which is particularly suitable for large-scale, high-throughput CTC detection needs.

[0069] Multi-channel synchronous automated processing avoids fatigue errors or operational inconsistencies that may occur when operators handle each sample separately. The waste removal of all samples is completed synchronously under the control of the equipment, reducing the total time and frequency of operators' exposure to potential biohazard environments, and systematically improving the overall laboratory biosafety level.

[0070] In some embodiments, see Figures 1-6The pipette 21 is mounted above or obliquely above the dyeing component. When the driving motor 24 drives the rotating frame 23 to rotate from the second position to the first position, the pipette 21 moves downward from the oblique upper or directly above position along an arc trajectory and is inserted into the dyeing component. After the suction is completed, the rotating frame 23 rotates in the opposite direction, and the pipette 21 is lifted along the original trajectory and finally returns to the initial position above the dyeing component.

[0071] The installation method of the pipette 21 obliquely above the staining component can utilize the longitudinal space of the equipment and avoid occupying too much area horizontally. It is particularly suitable for space-constrained scenarios of integrated laboratory equipment.

[0072] The high initial positioning allows the pipette 21 to be completely out of the staining component area during non-working periods, eliminating the risk of accidental collision with samples or instruments. At the same time, when the operator replaces the sample or maintains the staining component, there is no instrument obstruction or interference, further ensuring operational safety and convenience.

[0073] Furthermore, the design of inserting from an oblique upper part allows the end of the pipette 21 to contact the liquid surface at an inclined angle. Combined with negative pressure suction, it can reduce the generation of liquid surface vortices or bubbles, thereby improving the efficiency of waste liquid suction. When the pipette 21 is pulled out, the pipette 21 is lifted smoothly along an arc path, avoiding waste liquid splashing caused by vertical pull-out, significantly reducing the risk of cross-contamination, and ensuring the cleanliness of the equipment.

[0074] In some embodiments, see Figures 1-6 The dyeing component includes a dyeing tank 3 and a collecting tank 31. The collecting tank 31 is arranged on one side of the dyeing tank 3 and is connected to the dyeing tank 3. Both the collecting tank 31 and the dyeing tank 3 have upward notches, and the pipette 21 is inserted into or pulled out of the collecting tank 31 through the notch of the collecting tank 31.

[0075] Furthermore, the staining tank 3 is used to accommodate body fluid samples and perform target cell (such as CTC) staining reactions, and the collection tank 31 is used to collect waste liquid to be removed after staining. Both the staining tank 3 and the collection tank 31 are provided with upward notches to facilitate operation and the entry and exit of the pipette 21.

[0076] After dyeing is completed, the waste liquid naturally flows into or is guided from the dyeing tank 3 to the collecting tank 31 for temporary storage. When the flipping mechanism drives the pipette 21 to move, the end of the pipette 21 is accurately inserted into or pulled out of the collecting tank 31 through the upward notch of the collecting tank 31. When the rotating frame 23 is in the first position, the pipette 21 is inserted below the liquid surface of the waste liquid in the collecting tank 31; when it is rotated to the second position, the pipette 21 is completely pulled out of the collecting tank 31. After the waste liquid negative pressure pump is started, the negative pressure is transmitted to the pipette 21 through the pipeline to directly draw out the waste liquid in the collecting tank.

[0077] The collection tank 31 serves as an independent waste liquid temporary storage area, which physically separates the waste liquid from the sample in the staining tank 3. The pipette 21 only contacts the waste liquid in the collection tank 31, avoiding the suction process from disturbing the rare CTC cells that may exist in the staining tank 3, thereby ensuring the integrity of the test sample.

[0078] In some embodiments, see Figure 4 The connection between the collecting tank 31 and the dyeing tank 3 forms a U-shaped opening 33, and the U-shaped opening 33 is used to avoid the pipette 21.

[0079] Furthermore, the U-shaped opening 33 is located at the upper edge of the connecting part of the two grooves, forming a downwardly concave arc-shaped avoidance space. When the flipping mechanism drives the pipette 21 to insert or withdraw from the collection groove 31, the tube body or end of the pipette 21 faces the U-shaped opening 33 area in the motion trajectory.

[0080] During the process of the rotating frame 23 rotating from the second orientation to the first orientation, the pipette 21 is inserted from the upper oblique direction of the collecting tank 31, and its tube body descends along an arc path. At this time, the U-shaped opening 33 provides a lateral avoidance space to ensure that the pipette 21 enters the collecting tank 31 without collision. During the process of the rotating frame 23 rotating from the first orientation to the second orientation, the U-shaped opening 33 also eliminates physical interference for the lifting trajectory of the pipette 21 to prevent the pipette 21 from scraping the tank wall at the connection.

[0081] In some embodiments, the bottom wall of the collecting tank 31 transitions obliquely to the dyeing tank 3, and the bottom wall of the collecting tank 31 is lower than the bottom wall of the dyeing tank 3. The bottom wall of the collecting tank 31 transitions obliquely downward from the end away from the dyeing tank 3 to the connection point, forming a slope structure. The lowest point of the bottom wall of the collecting tank 31 is always lower than the bottom wall of the dyeing tank 3, forming a stable liquid level difference.

[0082] After the target cells are stained, the waste liquid generated by the staining naturally flows to the lowest point along the inclined bottom wall of the collection tank 31 under the action of gravity, that is, the area close to the U-shaped opening 33. Since the bottom wall of the collection tank 31 is lower than the staining tank 3 as a whole, the waste liquid in the staining tank 3 is completely collected into the collection tank 31 through the connecting point, reducing the risk of residue.

[0083] Furthermore, when the pipette 21 is inserted into the collection tank 31, the end of the pipette 21 is precisely positioned at the lowest point of the inclined bottom wall. After the waste liquid negative pressure pump is started, the waste liquid collected at the lowest point can be efficiently sucked out.

[0084] In some embodiments, see Figure 4 The bottom wall of the dyeing tank 3 is provided with a tray 34 protruding from the bottom wall and used to hold the filter membrane. The tray 34 and the bottom wall and side walls of the dyeing tank 3 are surrounded to form a diversion channel 35 for the waste liquid to flow to the collection tank 31.

[0085] Furthermore, a filter membrane for capturing target CTC cells is provided on the tray 34 , and a gap is formed between the tray 34 and the bottom wall and side walls of the staining tank 3 , and the gap is surrounded to form an annular or U-shaped guide channel 35 surrounding the tray 34 .

[0086] After dyeing is completed, the waste liquid permeates downward through the filter membrane and enters the area below the tray 34. The waste liquid flows into the diversion channel 35 along the edge of the tray 34. Under the action of gravity, it flows through the diversion channel 35 to the side wall of the dyeing tank 3 close to the collecting tank 31. The waste liquid finally flows into the collecting tank 31 with an inclined bottom wall and a low position through the connection between the collecting tank 31 and the dyeing tank 3.

[0087] The tray 34 physically lifts the filter membrane to separate it from the bottom wall of the staining tank 3, preventing the filter membrane from being soaked by the residual waste liquid at the bottom of the tank, ensuring the stable attachment of the target CTC cells. The diversion channel 35 formed by the tray 34 and the tank wall constitutes a directional waste liquid high-speed passage. Combined with the inclined bottom wall and low-position design of the collection tank 31, the waste liquid can be drained unobstructed from the bottom of the filter membrane, the diversion channel 35, and the collection tank 31 in sequence.

[0088] The diversion channel 35 allows the waste liquid to bypass the bottom of the filter membrane and be discharged, eliminating the risk of scouring or tearing the captured CTC cells when the waste liquid flows through the filter membrane, significantly improving the recovery rate of rare cells, and physically isolating the filter membrane from the waste liquid path, eliminating waste liquid backflow contamination, and ensuring the purity of subsequent test samples.

[0089] In some embodiments, see Figures 1-6 Multiple dyeing assemblies are arranged along the axial direction of the rotating frame 23, and the collection tank 31 and dyeing tank 3 of each dyeing assembly are arranged along the radial direction of the rotating frame 23. Among them, multiple dyeing assemblies can be arranged as an integrated structure. It should be noted that the multiple dyeing assemblies can be independent structures or integrated into an integrated dyeing table, and multiple sets of dyeing tanks 3 and collection tanks 31 are arranged on the integrated dyeing table.

[0090] Multiple staining components are closely arranged along the axial direction of the rotating frame 23 to form a sample channel for parallel processing. Inside each staining component, the staining tank 3 and the collection tank 31 are arranged side by side along the radial direction of the rotating frame 23, wherein the collection tank 31 is located on the side of the staining tank 3 close to the central axis of the rotating frame 23.

[0091] A radially extending pipette 21 is provided on the turret 23 corresponding to each dyeing assembly, and all pipettes 21 move synchronously with the turret 23. When the turret 23 rotates to the first position, all pipettes 21 are simultaneously inserted into the corresponding notches of the collection tank 31, and the U-shaped openings 33 are precisely positioned to avoid the pipettes 21.

[0092] The axially parallel dyeing components combined with the radial slot arrangement form a compact operating array around the rotating frame 23. A single rotation action triggers all channels to synchronously complete waste liquid suction, greatly improving operating efficiency.

[0093] In some embodiments, see Figure 3 、 Figure 5-Figure 6 The high-efficiency CTC detection integrated device also includes an enrichment component 4 and a liquid adding component 5. The enrichment component 4 is arranged on the base 1 and is used to enrich target cells. The liquid adding component 5 includes a first guide rail 51 arranged on the base 1 and a first liquid adding tube 52 and a second liquid adding tube 53 slidingly matched with the first guide rail 51. The first liquid adding tube 52 is used to add a first reagent to the staining component, and the second liquid adding tube 53 is used to add a second reagent to the enrichment component 4.

[0094] Furthermore, the liquid adding component 5 includes a first guide rail 51 arranged on the base 1 and a first liquid adding tube 52 and a second liquid adding tube 53 slidingly engaged with the first guide rail 51, the first liquid adding tube 52 is used to add the first reagent to the enrichment component 4, and the second liquid adding tube 53 is used to add the second reagent to the staining component.

[0095] The workflow for enrichment and staining is as follows:

[0096] First, the body fluid sample is placed in the enrichment component 4, then the first liquid adding tube 52 slides along the first guide rail 51 to a predetermined position above the enrichment component 4, and the first reagent is added thereto to start and complete the enrichment process of the target cells; the enriched target cells are transferred to the staining component, and the second liquid adding tube 53 slides along the first guide rail 51 to a predetermined position above the staining component, and the second reagent is added thereto to start and complete the staining process of the target cells. Finally, the stained cell sample can be used for subsequent identification and analysis.

[0097] Furthermore, the liquid adding component 5 also includes a first reagent bottle (not shown in the figure) for storing the first reagent; a first liquid injection pump (not shown in the figure) connected between the first reagent bottle and the first liquid adding tube 52 through a pipeline, for pumping the first reagent into the first liquid adding tube 52; a second reagent bottle (not shown in the figure) for storing the second reagent; a second liquid injection pump (not shown in the figure) connected between the second reagent bottle and the second liquid adding tube 53 through a pipeline, for pumping the second reagent into the second liquid adding tube 53.

[0098] When the first liquid adding tube 52 moves to above the target enrichment component 4, the first liquid injection pump starts to inject the reagent in the first reagent bottle into the enrichment component 4 through the first liquid adding tube 52; when the second liquid adding tube 53 moves to above the target staining component, the second liquid injection pump starts to inject the reagent in the second reagent bottle into the staining component through the second liquid adding tube 53.

[0099] It can be understood that although the first reagent bottle, the first infusion pump, the second reagent bottle, and the second infusion pump are not shown in the accompanying drawings, it does not affect the specific implementation of this embodiment. Those skilled in the art can adjust and set the above technical features based on the recorded content and actual needs.

[0100] Furthermore, the enrichment assembly 4 includes a connecting seat 41, a filter 42 and a filtrate negative pressure pump (not shown in the figure).

[0101] Among them, the connecting seat 41 is set on the base 1, and the connecting seat 41 has a channel running through from top to bottom; the filter membrane is placed inside the filter 42, and the bottom of the filter 42 is detachably connected to the top of the connecting seat 41 and connected to the channel, and the first injection pipe is connected to the opening at the top of the filter 42 to add the first reagent to the filter 42; the filtrate negative pressure pump is connected to the bottom of the connecting seat 41 through a pipeline and connected to the channel, which is used to provide negative pressure and suck the waste liquid in the filter 42.

[0102] After the filter 42 is installed on the connecting seat 41, the first liquid adding tube 52 is used to add the first reagent to the filter 42 through the opening at the top of the filter 42. Of course, the first liquid adding tube 52 can also be used to add a sample containing cells. The filtrate negative pressure pump is started, and the waste liquid is filtered through the filter membrane and then sucked out from the channel. The target cells are retained on the filter membrane to complete the enrichment.

[0103] The detachable design of the filter 42 in this embodiment facilitates quick replacement or cleaning of the filter membrane, and combined with negative pressure suction, accelerates liquid filtration and significantly improves cell enrichment efficiency.

[0104] It should be noted that the structure of the filter 42 may refer to the filter assembly in publication number CN220590059U.

[0105] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention in any form. Although the present invention has been disclosed as above with preferred embodiments, they are not intended to limit the present invention. Any technician familiar with this patent can make some changes or modifications to equivalent embodiments using the above technical content without departing from the scope of the technical solution of the present invention. The implementation schemes in the above embodiments can also be further combined or replaced. However, any simple modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the solution of the present invention.

Claims

1. A high-efficiency CTC detection integrated device, comprising a base and a staining component disposed on the base, the staining component being used to stain target cells, characterized in that: Also includes: A fixing frame is arranged on the base; The turning mechanism is connected to the fixed frame in a reciprocating and rotatable manner; A pipette is connected to the flip mechanism, which drives the pipette to be inserted into and removed from the dyeing assembly during reciprocating rotation; The waste liquid negative pressure pump is connected to the liquid suction component through a pipeline, and is used to provide negative pressure and suck the waste liquid in the dyeing component.

2. The high-efficiency CTC detection integrated device according to claim 1, characterized in that: The turning mechanism comprises: a rotating frame, wherein the pipette is arranged on the rotating frame; The driving motor is connected to the fixed frame and is used to drive the rotating frame to rotate back and forth along its own axis between a first position and a second position. During the process of the rotating frame rotating from the first position to the second position, the pipette is inserted into the dyeing component and gradually withdrawn from the dyeing component.

3. The high-efficiency CTC detection integrated device according to claim 2, characterized in that: The pipette extends along the radial direction of the rotating frame, and a plurality of pipettes are arranged on the rotating frame along the axial direction.

4. The high-efficiency CTC detection integrated device according to claim 3, characterized in that: The pipette is arranged above or obliquely above the dyeing component.

5. The high-efficiency CTC detection integrated device according to any one of claims 1 to 4, characterized in that: The dyeing component includes: dyeing tank; The collecting tank is arranged at one side of the dyeing tank and is communicated with the dyeing tank. Both the collecting tank and the dyeing tank have upward notches. The pipette is inserted into or withdrawn from the collecting tank through the notches of the collecting tank.

6. The high-efficiency CTC detection integrated device according to claim 5, characterized in that: The connection between the collecting tank and the dyeing tank forms a U-shaped opening, and the U-shaped opening is used to avoid the pipette.

7. The high-efficiency CTC detection integrated device according to claim 5, characterized in that: The bottom wall of the collecting trough is inclined to transition to the dyeing trough, and the bottom wall of the collecting trough is lower than the bottom wall of the dyeing trough.

8. The high-efficiency CTC detection integrated device according to claim 5, characterized in that: The bottom wall of the dyeing tank is provided with a tray protruding from the bottom wall and used for holding the filter membrane. The tray, the bottom wall and the side wall of the dyeing tank are surrounded to form a diversion channel for the waste liquid to flow to the collection tank.

9. The high-efficiency CTC detection integrated device according to claim 5, characterized in that: A plurality of dyeing components are arranged along the axial direction of the rotating frame, and the collecting tank and the dyeing tank of each dyeing component are arranged along the radial direction of the rotating frame.

10. The high-efficiency CTC detection integrated device according to claim 5, characterized in that: Also includes: An enrichment component is provided on the base and is used for enriching target cells; The liquid adding assembly includes a first guide rail arranged on the base and a first liquid adding tube and a second liquid adding tube slidingly matched with the first guide rail. The first liquid adding tube is used to add a first reagent to the staining assembly, and the second liquid adding tube is used to add a second reagent to the enrichment assembly.

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