Method for detecting miRNA and analogues thereof and use thereof
CN120665995BActive Publication Date: 2026-06-26SHANGHAI RUNDARONGJIA BIOLOGICAL TECH CO LTD
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- SHANGHAI RUNDARONGJIA BIOLOGICAL TECH CO LTD
- Filing Date
- 2025-06-30
- Publication Date
- 2026-06-26
AI Technical Summary
Technical Problem
Existing miRNA detection technologies exhibit poor specificity and a high proportion of non-specific signals when dealing with target miRNAs and miRNA analogs that differ by only one base, thus affecting the accuracy of the detection results.
Method used
Two DNA probes were designed: probe 1 was a perfect match for the miRNA analog and probe 2 was a perfect match for the target miRNA. By optimizing the hybridization temperature, probe 2 was prevented from hybridizing with the miRNA analog and was used to capture only the target miRNA, thus generating a fluorescent signal.
Benefits of technology
It improves the specificity of miRNA detection, reduces non-specific signals, enhances the ability to distinguish single bases, and improves the accuracy of detection results.
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Figure CN120665995B_ABST
Abstract
The application provides a detection method and application of miRNA and analogs thereof, and relates to the field of nucleic acid detection. The application designs probe 1 and probe 2 aiming at target miRNA and miRNA analogs; the probes combine with a part of miRNA respectively, probe 2 can be combined to a solid phase carrier such as a chip or a microsphere, and probe 1 is modified with signal labeling materials such as a fluorescent dye or biotin; after the target miRNA or miRNA analog is mixed with probe 1 and probe 2 and incubated, the probe 2 can not be hybridized with the miRNA analog by optimizing the hybridization temperature, only the target miRNA is hybridized and captured, and the detection specificity is high.
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