LAMP primer combination for detecting influenza A virus H3N2, kit and application

By designing a LAMP primer combination based on the conserved region of the HN gene and optimizing the reaction system, the problems of insufficient sensitivity and specificity in the detection of influenza A virus H3N2 in existing technologies were solved, achieving rapid and accurate detection results, and meeting the needs of clinical diagnosis and epidemiological monitoring.

CN120666118APending Publication Date: 2025-09-19SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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Patent Information

Application Number
CN202510895762.0
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-06-30
Publication Date
2025-09-19

AI Technical Summary

Technical Problem

The existing LAMP technology for detecting influenza A virus H3N2 has problems such as low sensitivity, insufficient specificity and high nonspecific amplification rate, which makes it difficult to meet the needs of rapid and accurate clinical diagnosis and epidemiological monitoring.

Method used

A LAMP primer combination based on the conserved region of 200-850 bp in the HN gene of influenza A virus H3N2 was designed, including outer primer F3, outer primer B3, inner primer FIP, inner primer BIP, loop primer LF, and loop primer LP. The concentration of the reaction system was optimized, and a loop-mediated isothermal amplification reaction was used in combination with a fluorescent dye and Bst DNA polymerase to achieve high sensitivity and specificity detection.

Benefits of technology

It achieves high-sensitivity (6.25 Copies/Reaction) and high-specificity (95% coincidence rate) detection in a short period of time, can quickly identify influenza A virus H3N2, and provides an efficient clinical diagnosis and epidemiological monitoring tool.

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Abstract

The invention relates to the technical field of biology, in particular to an LAMP primer combination for detecting influenza A virus H3N2, a kit and application. The invention provides an LAMP (Loop-Mediated Isothermal Amplification) primer combination for detecting an influenza A virus H3N2. The LAMP primer combination is designed on the basis of 200-850bp of an HN gene of the influenza A virus H3N2. The research and development of the invention find that multiple sequence alignment is carried out on 30 global H3N2 strains through Clustal Omega, and a highly conserved region, namely a nucleotide site 200-850bp (corresponding to an HA structural domain), is screened out. The LAMP primer combination designed on the basis of the highly conserved region can detect the influenza A virus H3N2 with high sensitivity and high specificity, and can rapidly detect the influenza A virus H3N2 in a short time.
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