Automatic sample feeding device and method for industrial wastewater anaerobic biotoxicity evaluation sample

Abstract

The present invention relates to the technical field of anaerobic biotoxicity experiments, and discloses an industrial wastewater anaerobic biotoxicity evaluation sample automatic feeding apparatus, which comprises a conical flask for temporarily storing an anaerobic sludge sample and a nutrient solution, and further comprises: a storage box, which is internally provided with a storage space for storing the anaerobic sludge sample and the nutrient solution; the gas storage tank is arranged on the side surface of the storage box, and high-pressure nitrogen is injected into the gas storage tank; the nitrogen conveying assembly is arranged on the gas storage tank, penetrates through and extends to the inner side of the storage box, and is used for aerating the anaerobic sludge sample in the storage box and homogenizing the sludge through stirring; the sample introduction and supply assemblies are arranged on the storage box and are communicated with the storage box, and the two sample introduction and supply assemblies are used for supplying the anaerobic sludge sample and the nutrient solution into the conical flask respectively. The automatic sample injection device and method for the industrial wastewater anaerobic biotoxicity evaluation sample aim at simplifying the exhaust process, reducing contact between microorganisms and air and improving the experiment efficiency and the result accuracy.

Description

Technical Field

[0001] The invention relates to the technical field of anaerobic biological toxicity experiments, in particular to an automatic sampling device and method for evaluating the anaerobic biological toxicity of industrial wastewater. Background Art

[0002] Industrial wastewater is generally characterized by large volumes, high toxicity, high concentrations, and significant difficulty in treatment. Anaerobic biological processes, as a key technology in sewage treatment plants, have been widely adopted for industrial wastewater treatment. However, the reaction system is susceptible to the impact of high concentrations of toxic substances in the wastewater, resulting in unstable treatment results and even system failure. Therefore, accurately assessing the biological toxicity of wastewater before it enters the sewage treatment plant is key to ensuring efficient and effective wastewater treatment operations throughout the entire process.

[0003] Existing anaerobic toxicity assessments of industrial wastewater primarily rely on anaerobic methanogenesis experiments. These tests involve mixing the wastewater to be evaluated with anaerobic microorganisms and quantifying the anaerobic toxicity by measuring methane production. Existing evaluation systems are manually assembled using equipment such as Erlenmeyer flasks, water baths, simple plastic tubes, and pedestal fermentation tubes. This operating mode presents two significant drawbacks: first, the system relies entirely on manual maintenance, resulting in low efficiency and a high risk of human error. Second, the system is poorly sealed, allowing air to easily infiltrate. This results in residual oxygen in the delivery pipes and Erlenmeyer flasks, significantly inhibiting anaerobic microbial activity and seriously interfering with the accuracy of experimental results. Furthermore, the precise dosing of anaerobic sludge, wastewater, and nutrient solution plays a crucial role in determining the evaluation results. However, existing technologies still rely on manual weighing, which is not only cumbersome but also significantly increases the risk of anaerobic sludge inactivation due to frequent exposure to air. Furthermore, ensuring high-precision volumetric measurement is difficult, resulting in poor reliability and reproducibility of experimental data. Based on the above problems, there is an urgent need to invent an anaerobic biological toxicity assessment system that can accurately sample and reduce the contact between air and anaerobic sludge, so as to achieve efficient biological toxicity assessment of industrial wastewater. Summary of the Invention

[0004] The purpose of the present invention is to simplify the exhaust process, reduce the contact between microorganisms and air, and improve experimental efficiency and result accuracy, and to propose an automatic sampling device and method for industrial wastewater anaerobic biological toxicity evaluation samples.

[0005] The technical solution of the present invention to solve the above technical problems is as follows:

[0006] An automatic sampling device for anaerobic biological toxicity evaluation of industrial wastewater samples, comprising a conical flask for temporarily storing anaerobic sludge samples and nutrient solution, and further comprising:

[0007] a storage tank having a storage space therein for storing the anaerobic sludge sample and the nutrient solution;

[0008] A gas storage tank is provided on the side surface of the storage box and is filled with high-pressure nitrogen;

[0009] A nitrogen delivery assembly is provided on the gas storage tank and penetrates and extends to the inner side of the storage box, and is used to aerate the anaerobic sludge sample in the storage box and achieve sludge homogenization through stirring;

[0010] A sampling supply assembly is provided on the storage box and is in communication with the storage box, wherein two sampling supply assemblies are provided to respectively supply the anaerobic sludge sample and the nutrient solution into the conical flask;

[0011] A gas drive assembly is connected to the storage box and is interconnected with the supply end of the sample supply assembly, and is used to locate and seal the opening of the conical flask and drive the supply end of the sample supply assembly to move toward the opening of the conical flask;

[0012] The pipeline connecting component is connected to the gas driving component and is interconnected with the sample supply component. It is used to replace the air in the sample supply component and the conical flask with nitrogen, eliminating the interference of air on the anaerobic sludge sample and the experimental process.

[0013] On the basis of the above technical solution, the present invention can also be improved as follows.

[0014] Furthermore, a partition perpendicular to the horizontal plane is fixedly installed inside the storage box, and the partition divides the internal area of ​​the storage box into an anaerobic sludge sample storage area and a nutrient solution storage area. Two feeding pipes that penetrate and extend to the inside of the storage box are also fixedly installed on the outside of the storage box, and the two feeding pipes correspond to the anaerobic sludge sample storage area and the nutrient solution storage area respectively.

[0015] Furthermore, the nitrogen delivery assembly includes:

[0016] The chamber is fixedly mounted on the inner wall of the storage box, and is connected to a first air pipe having one end extending through and outside the storage box, the other end of the first air pipe being connected to the air outlet of the gas storage tank, and an output port is provided on the surface of the chamber and is opposite to the first air pipe;

[0017] A first solenoid valve is provided on the first air pipe and is used to control the opening and closing of the first air pipe;

[0018] The pneumatic drive component is arranged on the inner side of the chamber. The pneumatic drive component is also provided with a stirring rod. The high-pressure nitrogen in the gas storage tank is input into the chamber through the first air pipe to drive the pneumatic drive component and the stirring rod to operate, so as to stir the anaerobic sludge to ensure the sludge homogeneity, and output from the output port to aerate the anaerobic sludge sample in the storage box.

[0019] Furthermore, the pneumatic drive component includes:

[0020] a first gear rotatably connected to the inner side of the chamber;

[0021] A shaft is rotatably connected to the inner side of the chamber and one end of the shaft extends through the outer side of the chamber, and the end of the stirring rod is fixedly connected to the end of the shaft outside the chamber;

[0022] The second gear is fixedly mounted on the outer side of the shaft and meshes with the first gear.

[0023] Furthermore, the sample supply assembly includes:

[0024] A pump body is fixedly mounted on the outside of the storage tank, wherein the suction end of the pump body is connected to a suction pipe having one end passing through and extending into the interior of the storage tank. The number of the pump body and the number of the suction pipe are both two, and the two suction pipes are respectively arranged in the anaerobic sludge sample storage area and the nutrient solution storage area;

[0025] A communication seat is provided on the output end of the pump body and is communicated with the output end of the pump body;

[0026] A filling pipe, one end of which is connected to the connecting seat. The number of the filling pipes is not less than eight and is divided into two equal groups, respectively provided on the two connecting seats. The outer sides of two adjacent filling pipes are fixedly connected to the rubber stoppers. There are four rubber stoppers, and each rubber stopper is provided with two filling pipes respectively from the two connecting seats;

[0027] The flow valve is fixedly installed on the outside of the filling pipe, and its number and distribution position are matched one by one with the filling pipe.

[0028] Furthermore, the gas drive assembly includes:

[0029] A main air outlet pipe is fixedly installed on the outside of the storage box and is interconnected with the anaerobic sludge sample storage area;

[0030] A piston cylinder is fixedly mounted on the outside of the storage box. A piston rod is provided on the inside of the piston cylinder, one end of which passes through and extends to the outside of the piston cylinder. The piston rod can be telescopically displaced along the inside of the piston cylinder. There are two piston cylinders, and the two piston cylinders are connected to each other through a connecting pipe.

[0031] An elastic member, one end of which is fixedly mounted on the inner side of the piston cylinder and the other end of which is fixedly connected to the end of the piston rod;

[0032] a second air pipe, one end of which is connected to the main air outlet pipe and the other end of which is connected to the piston cylinder; a second solenoid valve is further provided on the outer side of the second air pipe;

[0033] A deflation solenoid valve is provided on the piston cylinder and is in communication with the piston cylinder;

[0034] The mounting plate is fixedly mounted on one end portion of the piston rod located outside the piston cylinder, and the rubber plug and the mounting plate are fixedly connected to each other.

[0035] Furthermore, the pipeline communication component includes:

[0036] a third air outlet pipe, one end of which is connected to the main air outlet pipe, and the other end of which passes through and extends into one of the connecting seats, wherein the third air outlet pipe is provided with a third solenoid valve;

[0037] The interconnecting pipe is connected between the two connecting seats, and a fourth solenoid valve is provided on the outside of the interconnecting pipe.

[0038] Furthermore, an output tube is provided through the rubber stopper, and the other end of the output tube is connected to a gas quantity measuring component.

[0039] Furthermore, the gas storage tank is also provided with a gas filling port.

[0040] An automatic sampling method for evaluating the anaerobic toxicity of industrial wastewater comprises the following steps:

[0041] S10. Adding the anaerobic sludge sample and the nutrient solution to the anaerobic sludge sample storage area and the nutrient solution storage area in the storage box through the feeding pipe, respectively. Opening the first solenoid valve, the high-pressure nitrogen in the gas storage tank is input into the chamber of the nitrogen delivery assembly through the first air pipe, driving the first gear and the second gear of the pneumatic drive member to engage with each other, driving the shaft to rotate axially, causing the stirring rod to stir the anaerobic sludge to ensure the sludge is homogenized. Simultaneously, nitrogen is output from the output port of the chamber to aerate the anaerobic sludge sample in the storage box.

[0042] S20. Open the second solenoid valve of the gas drive assembly. The nitrogen in the anaerobic sludge sample storage area is output to the inner side of the piston cylinder through the main gas outlet pipe and the second gas pipe. Under the action of gas pressure, the piston rod is pushed outward. The conical flask is placed directly under the rubber stopper. After the piston rod is fully extended, the second solenoid valve and the air release solenoid valve are closed.

[0043] S30, open the venting solenoid valve, the gas in the piston cylinder is discharged, and the rubber stopper is buckled downward at the opening of the conical flask under the action of the elastic member;

[0044] S40. Open the third and fourth solenoid valves of the pipeline connection assembly. The nitrogen in the anaerobic sludge sample storage area is output to the inside of one of the connecting seats through the main outlet pipe and the third outlet pipe. The other connecting seat is then filled with nitrogen through the interconnecting pipe. Nitrogen is then injected into the conical flask from the connecting seat and the filling pipe, replacing the air in the connecting seat, the filling pipe, and the conical flask with nitrogen. During this process, the gas volume measurement assembly connected to the output pipe on the rubber stopper measures whether the air has been completely replaced.

[0045] S50. The pump body and flow valve of the sampling supply assembly are turned on. The two pump bodies respectively draw in the anaerobic sludge sample and nutrient solution from the anaerobic sludge sample storage area and nutrient solution storage area of ​​the storage tank through the suction pipes, and inject them into the conical flask through the connecting seat and the filling pipe, thereby completing the automatic sampling of samples for anaerobic toxicity evaluation of industrial wastewater.

[0046] Compared with the prior art, the technical solution of this application has the following beneficial technical effects:

[0047] In the automatic sampling device for anaerobic biological toxicity evaluation of industrial wastewater of the present invention, a storage box can store anaerobic sludge samples and nutrient solution, and a gas storage tank cooperates with a nitrogen delivery component to use high-pressure nitrogen to aerate and stir the anaerobic sludge sample in the storage box to achieve sludge homogenization. On the one hand, the uniformity of the sludge sample can be ensured and the reliability of the experimental results can be improved. On the other hand, the aeration process can reduce the residual air in the sludge to a certain extent, reducing the influence of air on microbial activity. Two sampling supply components respectively supply anaerobic sludge samples and nutrient solution into the conical flask to ensure the consistency of the sample and nutrient solution amounts. The gas drive component carries the conical flask and drives the supply end of the supply component to move. Combined with the pipeline connection component, the air in the sampling supply component and the conical flask can be automatically replaced with nitrogen. The overall process not only simplifies the process and improves the experimental efficiency, but also ensures the consistency and thoroughness of the exhaust operation, effectively eliminates the interference of air on the anaerobic sludge sample and the experimental process, ensures the activity of anaerobic microorganisms, and thus improves the accuracy and reliability of the experimental results of anaerobic toxicity evaluation of industrial wastewater. BRIEF DESCRIPTION OF THE DRAWINGS

[0048] Figure 1 This is a schematic diagram of the overall connection structure of the present invention;

[0049] Figure 2 A schematic diagram of the connection structure of another perspective of the present invention;

[0050] Figure 3 This is a schematic diagram of the internal cross-sectional connection structure of the storage box of the present invention;

[0051] Figure 4 This is a schematic diagram of the connection structure between the storage box and the feeding pipe of the present invention;

[0052] Figure 5 This is a schematic diagram of the connection structure of some gas drive components of the present invention;

[0053] Figure 6 Schematic diagram of the internal structure of the chamber of the present invention.

[0054] Figure: 1, Erlenmeyer flask; 2, storage box; 3, gas storage tank; 4, nitrogen delivery assembly; 41, chamber; 42, first air pipe; 43, output port; 44, first solenoid valve; 45, pneumatic drive member; 451, first gear; 452, shaft; 453, second gear; 46, stirring rod; 5, sample supply assembly; 51, pump body; 52, suction pipe; 53, connecting seat; 54, filling pipe; 55, rubber stopper; 56, flow valve ; 6. Gas drive assembly; 61. Main air outlet pipe; 62. Piston cylinder; 63. Piston rod; 64. Elastic member; 65. Second air pipe; 66. Second solenoid valve; 67. Deflation solenoid valve; 68. Mounting plate; 7. Pipeline connection assembly; 71. Third air outlet pipe; 72. Third solenoid valve; 73. Intercommunication pipe; 74. Fourth solenoid valve; 8. Partition; 9. Feeding pipe; 10. Output pipe; 11. Gas quantity measurement assembly; 12. Gas filling port. DETAILED DESCRIPTION

[0055] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0056] Combine Figures 1-6 As shown, the automatic sampling device for anaerobic biological toxicity evaluation of industrial wastewater of the present invention comprises a conical flask 1 for temporarily storing anaerobic sludge samples and nutrient solution, and further comprises:

[0057] a storage tank 2, which is provided with a storage space for storing anaerobic sludge samples and nutrient solution;

[0058] The gas storage tank 3 is provided on the side surface of the storage box 2 and is filled with high-pressure nitrogen;

[0059] A nitrogen delivery assembly 4 is provided on the gas storage tank 3 and penetrates and extends to the inner side of the storage box 2. The nitrogen delivery assembly 4 is used to aerate the anaerobic sludge sample in the storage box 2 and achieve sludge homogenization by stirring;

[0060] A sample feeding assembly 5 is provided on the storage box 2 and is in communication with the storage box 2, wherein two sample feeding assemblies 5 are provided to respectively supply the anaerobic sludge sample and the nutrient solution into the conical flask 1;

[0061] The gas drive assembly 6 is connected to the storage box 2 and is interconnected with the supply end of the sample supply assembly 5. The gas drive assembly 6 is used to locate and seal the opening of the conical flask 1 and drive the supply end of the sample supply assembly 5 to move toward the opening of the conical flask 1.

[0062] The pipeline connecting component 7 is connected to the gas driving component 6 and is interconnected with the sample supply component 5. It is used to replace the air in the sample supply component 5 and the conical flask 1 with nitrogen, eliminating the interference of air on the anaerobic sludge sample and the experimental process.

[0063] When the automatic sampling device for anaerobic biological toxicity evaluation of industrial wastewater samples is working, the anaerobic sludge sample and the nutrient solution are first stored in the storage space of the storage box 2 respectively. The high-pressure nitrogen stored in the gas tank 3 penetrates into the inner side of the storage box 2 through the nitrogen delivery component 4, aerating the anaerobic sludge sample on the one hand and driving the internal stirring structure to operate to achieve sludge homogenization and ensure the uniformity of the sludge sample. Before sampling, the gas drive component 6 first carries the conical flask 1, and the pipeline connection component 7 is started. The nitrogen provided in the storage box 2 or the gas tank 3 is used to replace the air inside the sampling supply component 5 and the conical flask 1 with nitrogen, eliminating the interference of air on the anaerobic sludge sample and the subsequent experimental process, and creating a stable anaerobic environment. After the replacement is completed, the two sampling supply components 5 respectively draw the anaerobic sludge sample and nutrient solution from the storage box 2 and prepare to supply them into the conical flask 1, completing the automatic sampling operation and providing sample preparation for the subsequent accurate industrial wastewater anaerobic toxicity evaluation experiment.

[0064] In a preferred embodiment, the present invention can be further configured as follows: Figure 3 、 Figure 4 As shown, the interior of the storage box 2 is fixedly installed with a partition 8 perpendicular to the horizontal plane. The partition 8 divides the internal area of ​​the storage box 2 into an anaerobic sludge sample storage area and a nutrient solution storage area. Two feeding pipes 9 that penetrate and extend to the inside of the storage box 2 are also fixedly installed on the outside of the storage box 2. The two feeding pipes 9 correspond to the anaerobic sludge sample storage area and the nutrient solution storage area respectively. The partition 8 inside the storage box 2 is fixedly installed perpendicular to the horizontal plane, dividing the interior of the storage box 2 into two independent and equal-sized areas, respectively used to store anaerobic sludge samples and nutrient solution. During the experimental preparation stage, the operator can add corresponding substances to the anaerobic sludge sample storage area and the nutrient solution storage area respectively through the two corresponding feeding pipes 9 on the outside of the storage box 2. Due to the separation effect of the partition 8, the anaerobic sludge sample and the nutrient solution can be prevented from mixing with each other in the storage box 2, maintaining the independence of the two substances.

[0065] In a preferred embodiment, the present invention can be further configured as follows: Figure 3 、 Figure 6 As shown; the nitrogen delivery assembly 4 includes:

[0066] The chamber 41 is fixedly mounted on the inner wall of the storage box 2. A first air pipe 42 is connected to the chamber 41, one end of which passes through and extends to the outside of the storage box 2. The other end of the first air pipe 42 is connected to the air outlet of the air storage tank 3. An output port 43 is also provided on the surface of the chamber 41 and is opposite to the first air pipe 42.

[0067] A first solenoid valve 44 is provided on the first air pipe 42 to control the opening and closing of the first air pipe 42;

[0068] The pneumatic drive component 45 is arranged on the inner side of the chamber 41. The pneumatic drive component 45 is also provided with a stirring rod 46. The high-pressure nitrogen in the gas storage tank 3 is input into the chamber 41 through the first air pipe 42 to drive the pneumatic drive component 45 and the stirring rod 46 to operate, so as to stir the anaerobic sludge to ensure the sludge is homogeneous, and output from the output port 43 to aerate the anaerobic sludge sample in the storage box 2. When the anaerobic sludge sample in the storage box 2 needs to be processed, the first solenoid valve 44 is opened, and the high-pressure nitrogen in the gas storage tank 3 is transported to the chamber 41 fixedly installed on the inner wall of the storage box 2 through the first air pipe 42. The high-pressure nitrogen entering the chamber 41 drives the pneumatic drive component 45 to operate. Since the stirring rod 46 is arranged on the pneumatic drive The pneumatic drive component 45 drives the stirring rod 46 to rotate synchronously, thereby stirring the anaerobic sludge in the storage box 2, achieving sludge homogenization, ensuring that the anaerobic sludge sample is evenly mixed, and improving the consistency of the sample and the reliability of the experimental results. At the same time, after the high-pressure nitrogen completes the driving stirring function in the chamber 41, it is discharged from the output port 43 arranged opposite to the first air pipe 42 to aerate the anaerobic sludge sample in the storage box 2. The aeration process can provide a certain gas environment for the anaerobic sludge on the one hand, and on the other hand, help to further disperse the sludge, enhance the stirring and homogenizing effect, and reduce the residual air that may exist in the sludge, thereby reducing the impact of air on the activity of anaerobic microorganisms.

[0069] In a preferred embodiment, the present invention can be further configured as follows: Figure 3 、 Figure 6 Pneumatic drive member 45 includes:

[0070] The first gear 451 is rotatably connected to the inner side of the chamber 41;

[0071] The shaft 452 is rotatably connected to the inner side of the chamber 41 and one end thereof extends through and outside the chamber 41. The end of the stirring rod 46 is fixedly connected to the end of the shaft 452 located outside the chamber 41.

[0072] The second gear 453 is fixedly mounted on the outside of the shaft 452 and meshes with the first gear 451. When the high-pressure nitrogen in the gas tank 3 enters the chamber 41 through the first air pipe 42, the airflow with a certain pressure and flow rate directly acts on the first gear 451 connected to the inner side of the chamber 41, pushing the first gear 451 and the second gear 453 to start rotating. The rotation of the second gear 453 drives the shaft 452 to rotate axially, thereby driving the stirring rod 46 to perform a circular motion around the axis of the shaft 452. It should also be noted that the chamber 41, the first gear 451, and the second gear 453 have the same structure and principle as the gear-type pneumatic motor in the prior art, which will not be repeated here. The direction of the airflow is as follows: Figure 6 As shown, in the direction of the airflow, the first gear 451 and the second gear 453 can be driven to engage and rotate with each other.

[0073] In a preferred embodiment, the present invention can be further configured as follows: Figure 2 、 Figure 6 As shown; the sample supply assembly 5 includes:

[0074] The pump body 51 is fixedly mounted on the outside of the storage tank 2. The suction end of the pump body 51 is connected to a suction pipe 52 having one end extending through and into the interior of the storage tank 2. There are two pump bodies 51 and two suction pipes 52. The two suction pipes 52 are respectively arranged in the anaerobic sludge sample storage area and the nutrient solution storage area. The suction pipe 52 is closely attached to the inner wall of the storage tank 2 so as not to affect the normal operation of the stirring rod 46.

[0075] The communication seat 53 is provided on the output end of the pump body 51 and is in communication with the output end of the pump body 51;

[0076] A filling tube 54, one end of which is connected to the connecting seat 53. The number of filling tubes 54 is no less than eight and is divided into two equal groups, respectively provided on the two connecting seats 53. The outer sides of two adjacent filling tubes 54 are fixedly connected to rubber stoppers 55. There are four rubber stoppers 55, each of which is provided with two filling tubes 54 from two connecting seats 53. The rubber stoppers 55 are adapted to the caliber of the conical flask 1.

[0077] The flow valve 56 is fixedly installed on the outside of the filling pipe 54, and its number and distribution position are adapted to the filling pipe 54 one by one. The two pump bodies 51 are fixed on the outside of the storage box 2, and their suction ends penetrate into the anaerobic sludge sample storage area and the nutrient solution storage area in the storage box 2 respectively through the suction pipe 52. When the pump body 51 is started, the negative pressure suction is used to draw the anaerobic sludge sample and nutrient solution from the corresponding storage area through the suction pipe 52, and transport them to the output end of the pump body 51. The connecting seat 53 connected to the output end of the pump body 51 plays the role of gathering and diverting, and introduces the sample and nutrient solution output by the pump body 51 into multiple filling pipes 54. No less than eight filling pipes 54 are equally divided into two groups, which are respectively connected to the two connecting seats 53. These filling pipes 54 further transport the sample and nutrient solution into the conical flask 1, and the two adjacent filling pipes 54 are connected. The outer side of the filling tube 54 is fixed to the rubber stopper 55. The four rubber stoppers 55 can be adapted to the bottle mouth of the conical flask 1. The two filling tubes 54 on each rubber stopper 55 correspond to the delivery channels of the anaerobic sludge sample and the nutrient solution, respectively, to achieve synchronous filling of the conical flask 1. The flow valve 56 fixedly installed on the outer side of the filling tube 54 can accurately adjust the flow and delivery volume of the sample and nutrient solution in the filling tube 54 according to the experimental requirements. By controlling the opening of the flow valve 56, on the one hand, it can ensure that the amount of sludge samples and nutrient solution in the experimental group and each control group remains small, avoiding excessive contact between microorganisms and air due to manual weighing. On the other hand, it can also ensure that the appropriate amount of sample and nutrient solution is injected into the conical flask 1 within the specified time, providing accurate and qualified samples for the anaerobic toxicity evaluation experiment of industrial wastewater. The flow rate of the two pump bodies 51 is the same.

[0078] In a preferred embodiment, the present invention can be further configured as follows: Figure 1 、 Figure 5 As shown; the gas drive assembly 6 includes:

[0079] The main air outlet pipe 61 is fixedly installed on the outside of the storage box 2 and is connected to the anaerobic sludge sample storage area;

[0080] The piston cylinder 62 is fixedly mounted on the outside of the storage box 2. A piston rod 63 is provided on the inside of the piston cylinder 62, one end of which passes through and extends to the outside of the piston cylinder 62. The piston rod 63 can be telescopically displaced along the inside of the piston cylinder 62. There are two piston cylinders 62, and the two piston cylinders 62 are connected to each other through a connecting pipe.

[0081] An elastic member 64, one end of which is fixedly mounted on the inner side of the piston cylinder 62, and the other end of which is fixedly connected to the end of the piston rod 63;

[0082] A second air pipe 65 , one end of which is connected to the main air outlet pipe 61 and the other end of which is connected to the piston cylinder 62 . A second solenoid valve 66 is also provided on the outside of the second air pipe 65 ;

[0083] The deflation solenoid valve 67 is provided on the piston cylinder 62 and is in communication with the piston cylinder 62;

[0084] The mounting plate 68 is fixedly mounted on the end of the piston rod 63 on the outside of the piston cylinder 62. The rubber plug 55 and the mounting plate 68 are fixedly connected to each other. In the preparation stage of sampling the industrial wastewater anaerobic toxicity assessment sample, the gas drive assembly 6 starts to operate, opens the second solenoid valve 66, and the nitrogen in the anaerobic sludge sample storage area of ​​the storage tank 2 enters the inner side of the piston cylinder 62 through the main outlet pipe 61 and the second air pipe 65. Since the two piston cylinders 62 are interconnected through the connecting pipe, the nitrogen enters the two piston cylinders 62 evenly. The gas pressure pushes the piston rod 63 to overcome the resistance of the elastic member 64 and extend outward along the piston cylinder 62. The mounting plate 68 fixed on the outer end of the piston rod 63 drives the rubber plug 5 connected thereto. 5 rises. At this time, the operator can place the conical flask 1 just below the rubber stopper 55. When the piston rod 63 is fully extended to the outside of the piston cylinder 62, the second solenoid valve 66 and the venting solenoid valve 67 are closed to prevent nitrogen from continuing to enter the piston cylinder 62 and prevent the internal gas from leaking out. The piston rod 63 is kept in the extended state so that the conical flask 1 can be placed. After the conical flask 1 is placed, the venting solenoid valve 67 is opened to discharge the nitrogen in the piston cylinder 62, and the internal air pressure drops. The elastic member 64 loses the air pressure restraint and returns to its original state. The elastic force generated pulls the piston rod 63 back into the piston cylinder 62, driving the mounting plate 68 and the rubber stopper 55 to move downward, so that the rubber stopper 55 is tightly fastened to the opening of the conical flask 1.

[0085] In a preferred embodiment, the present invention can be further configured as follows: Figure 2 、 Figure 4 As shown; the pipeline connecting component 7 includes:

[0086] A third air outlet pipe 71 , one end of which is connected to the main air outlet pipe 61 , and the other end of which passes through and extends into one of the communication seats 53 . A third solenoid valve 72 is provided on the third air outlet pipe 71 ;

[0087] The interconnecting pipe 73 is connected between the two connecting seats 53. A fourth solenoid valve 74 is provided on the outside of the interconnecting pipe 73. In the sampling preparation stage of the industrial wastewater anaerobic toxicity evaluation experiment, the pipeline connecting component 7 is started to eliminate the air interference in the sampling system, and the third solenoid valve 72 and the fourth solenoid valve 74 are opened. The nitrogen in the anaerobic sludge sample storage area of ​​the storage tank 2 enters one of the connecting seats 53 through the main outlet pipe 61 and the third outlet pipe 71. Since the two connecting seats 53 are connected by the interconnecting pipe 73, the nitrogen quickly diffuses to the other connecting seat 53, so that the two connecting seats 53 are filled with nitrogen. Subsequently, the nitrogen is injected into the filling pipe 54 connected to the connecting seat 53. In the conical flask 1 that is in place and sealed by the rubber stopper 55, as nitrogen is continuously injected, the original air in the connecting seat 53, the filling pipe 54 and the conical flask 1 is gradually replaced and discharged, forming a high-purity nitrogen environment, effectively avoiding the inhibitory effect of residual air on the microbial activity in the anaerobic sludge sample. During the sampling stage, the fourth solenoid valve 74 is closed, so that the two connecting seats 53 operate independently, ensuring that the anaerobic sludge sample and the nutrient solution do not interfere with each other when they are injected into the conical flask 1 through their respective filling pipes 54, thereby preventing cross contamination. The third solenoid valve 72 controls the on and off of nitrogen and closes after the air replacement is completed to prevent nitrogen from affecting the fluid delivery pressure and flow stability during the subsequent sampling process.

[0088] In a preferred embodiment, the present invention can be further configured as follows: Figure 1 、 Figure 2 As shown; the rubber stopper 55 is also provided with an output pipe 10, and the other end of the output pipe 10 is connected to the gas quantity measuring component 11. In the air replacement link of the industrial wastewater anaerobic toxicity evaluation experiment, when the pipeline connecting component 7 injects nitrogen into the connecting seat 53, the filling pipe 54 and the conical flask 1 to replace the air, the output pipe 10 provided on the rubber stopper 55 is connected to the gas quantity measuring component 11, playing a key monitoring role. After the nitrogen enters the system, it pushes the original air to be discharged from the output pipe 10 and flows to the gas quantity measuring component 11. The gas quantity measuring component 11 monitors the flow rate, volume and other parameters of the discharged gas in real time. By analyzing these data, it is judged that the air in the connecting seat 53, the filling pipe 54 and the conical flask 1 is empty. Whether the air has been completely replaced by nitrogen. If the measurement data shows that the gas composition is stable and meets the indicators of pure nitrogen, it indicates that the air replacement is complete; if the data is abnormal, it means that there is still residual air. At this time, the nitrogen injection amount and replacement time can be adjusted until the gas amount measurement component 11 feedback shows that the air is completely replaced. In this way, through the cooperation of the output pipe 10 and the gas amount measurement component 11, the oxygen-free environment required for the experiment is ensured, and the influence of residual air on the activity of anaerobic microorganisms is effectively avoided, and the interference with the accuracy of the anaerobic toxicity evaluation results of industrial wastewater is reduced. The gas amount measurement component 11 is one of a gas flow meter and a gas analyzer. The gas amount measurement component 11 is connected to the end of the output pipe 10 through a quick-release connector.

[0089] In a preferred embodiment, the present invention can be further configured as follows: Figure 1 、 Figure 2 As shown; a gas filling port 12 is also provided on the gas storage tank 3. During the anaerobic toxicity evaluation experiment of industrial wastewater, the gas storage tank 3 serves as a storage container for high-pressure nitrogen. The nitrogen inside it will be continuously consumed as operations such as aeration and air replacement are performed in the storage box 2. When the nitrogen reserve in the gas storage tank 3 is insufficient and it is difficult to meet the nitrogen demand of subsequent experiments, the gas filling port 12 comes into play. The operator can connect the special nitrogen delivery equipment to the gas filling port 12, and use the equipment to inject high-pressure nitrogen into the gas storage tank 3 to replenish the nitrogen reserve in the tank. The nitrogen is replenished in time through the gas filling port 12, which can ensure that the nitrogen delivery component 4 continues to aerate and stir the anaerobic sludge sample in the storage box 2 to achieve sludge homogenization, and ensure that the pipeline connection component 7 smoothly replaces the air in the sample supply component 5 and the conical flask 1 with nitrogen, thereby maintaining the stable operation of the entire sample automatic sampling device.

[0090] The storage box 2 is provided with a partition 8 to separate the areas and is equipped with a feeding pipe 9, which can independently store anaerobic sludge samples and nutrient solution to prevent the two from mixing and deteriorating and reduce the chance of contact with the outside air; the gas tank 3 and the nitrogen delivery component 4 use high-pressure nitrogen to aerate the sludge and drive the stirring rod 46 to achieve sludge homogenization, which not only ensures the uniformity of the sample but also reduces the influence of air on the activity of anaerobic microorganisms; the sample supply component 5 adopts a double pump body 51 with a flow valve 56 to accurately control the sample volume, avoid frequent contact between microorganisms and air caused by manual weighing, and ensure that the samples of the experimental group and the control group are uniform. The sample quantity is consistent, reducing experimental errors; the gas drive component 6 drives the piston rod 63 with the help of nitrogen pressure to realize the automatic fixation and sealing of the rubber stopper 55 to the conical flask 1, simplifying the operation process; the pipeline connection component 7 uses nitrogen to replace the air in the sampling system, and cooperates with the output tube 10 on the rubber stopper 55 and the gas quantity measurement component 11 to accurately monitor the degree of air replacement and create a stable anaerobic environment; the gas filling port 12 of the gas storage tank 3 ensures a continuous supply of nitrogen to maintain stable operation of the device. The device has a compact structure and can be integrated into modules, which is convenient for batch preparation and on-site deployment.

[0091] An automatic sampling method for evaluating the anaerobic toxicity of industrial wastewater comprises the following steps:

[0092] S10. The anaerobic sludge sample and the nutrient solution are added to the anaerobic sludge sample storage area and the nutrient solution storage area in the storage box 2 respectively through the feeding pipe 9. The first solenoid valve 44 is opened, and the high-pressure nitrogen in the gas storage tank 3 is input into the chamber 41 of the nitrogen delivery assembly 4 through the first air pipe 42. The first gear 451 and the second gear 453 of the pneumatic driving member 45 are driven to engage with each other, driving the shaft 452 to rotate axially, so that the stirring rod 46 stirs the anaerobic sludge to ensure the sludge is homogenized. At the same time, the nitrogen is output from the output port 43 of the chamber 41 to aerate the anaerobic sludge sample in the storage box 2.

[0093] S20: Open the second solenoid valve 66 of the gas drive assembly 6. The nitrogen in the anaerobic sludge sample storage area is output to the inner side of the piston cylinder 62 through the main gas outlet pipe 61 and the second gas pipe 65. Under the action of the gas pressure, the piston rod 63 is pushed outward, and the conical flask 1 is placed directly under the rubber stopper 55. After the piston rod 63 is fully extended, the second solenoid valve 66 and the air release solenoid valve 67 are closed.

[0094] S30, open the venting solenoid valve 67, the gas in the piston cylinder 62 is discharged, and the rubber stopper 55 is buckled downward at the opening of the conical flask 1 under the action of the elastic member 64;

[0095] S40: Open the third solenoid valve 72 and the fourth solenoid valve 74 of the pipeline connecting assembly 7. The nitrogen in the anaerobic sludge sample storage area is output to the inner side of one of the connecting seats 53 through the main outlet pipe 61 and the third outlet pipe 71. The other connecting seat 53 is also filled with nitrogen through the interconnecting pipe 73. The nitrogen is then injected into the conical flask 1 through the connecting seat 53 and the filling pipe 54, replacing the air in the connecting seat 53, the filling pipe 54, and the conical flask 1 with nitrogen. During this process, the gas quantity measuring assembly 11 connected to the output pipe 10 on the rubber stopper 55 measures whether the air has been completely replaced.

[0096] S50. The pump body 51 and the flow valve 56 of the sample supply assembly 5 are opened. The two pump bodies 51 respectively draw in the anaerobic sludge sample and the nutrient solution from the anaerobic sludge sample storage area and the nutrient solution storage area of ​​the storage box 2 through the suction pipe 52, and inject them into the conical flask 1 through the connecting seat 53 and the filling pipe 54, completing the automatic sampling of samples for anaerobic toxicity evaluation of industrial wastewater.

[0097] It should be noted that, in this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply the existence of any such actual relationship or order between these entities or operations. Moreover, the terms "comprises," "comprising," or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article, or device comprising a series of elements includes not only those elements, but also other elements not explicitly listed, or elements inherent to such process, method, article, or device. In the absence of further limitations, an element defined by the phrase "comprising a ..." does not exclude the presence of other identical elements in the process, method, article, or device comprising the element.

[0098] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. An automatic sampling device for anaerobic biological toxicity evaluation of industrial wastewater, comprising a conical flask (1) for temporarily storing anaerobic sludge samples and nutrient solution, characterized in that: Also includes: A storage tank (2) is provided with a storage space for storing the anaerobic sludge sample and the nutrient solution; A gas storage tank (3) is provided on the side surface of the storage box (2), and high-pressure nitrogen is injected into the gas storage tank; A nitrogen delivery assembly (4) is provided on the gas storage tank (3) and penetrates and extends to the inner side of the storage box (2), and is used to aerate the anaerobic sludge sample in the storage box (2) and achieve sludge homogenization through stirring; A sample feeding assembly (5) is provided on the storage box (2) and is in communication with the storage box (2), wherein two sample feeding assemblies (5) are provided to respectively feed the anaerobic sludge sample and the nutrient solution into the conical flask (1); A gas drive assembly (6) is connected to the storage box (2) and is interconnected with the supply end of the sample supply assembly (5), and is used to locate and seal the opening of the conical flask (1) and drive the supply end of the sample supply assembly (5) to move toward the opening of the conical flask (1); The pipeline connecting component (7) is connected to the gas driving component (6) and is interconnected with the sample supply component (5). It is used to replace the air in the sample supply component (5) and the conical flask (1) with nitrogen, thereby eliminating the interference of air on the anaerobic sludge sample and the experimental process.

2. The automatic sampling device for anaerobic biological toxicity evaluation of industrial wastewater according to claim 1, characterized in that: A partition (8) perpendicular to the horizontal plane is fixedly installed inside the storage box (2), and the partition (8) divides the internal area of ​​the storage box (2) into an anaerobic sludge sample storage area and a nutrient solution storage area. Two feeding pipes (9) penetrating and extending to the inside of the storage box (2) are also fixedly installed on the outside of the storage box (2), wherein the two feeding pipes (9) correspond to the anaerobic sludge sample storage area and the nutrient solution storage area respectively.

3. The automatic sampling device for anaerobic biological toxicity evaluation of industrial wastewater according to claim 1, characterized in that: The nitrogen delivery assembly (4) comprises: The chamber (41) is fixedly mounted on the inner wall of the storage box (2). The chamber (41) is also connected to a first air pipe (42) having one end penetrating and extending to the outside of the storage box (2). The other end of the first air pipe (42) is connected to the air outlet of the air storage tank (3). An output port (43) is also provided on the surface of the chamber (41) and is opposite to the first air pipe (42). A first solenoid valve (44) is provided on the first air pipe (42) and is used to control the opening and closing of the first air pipe (42); A pneumatic drive component (45) is provided inside the chamber (41). A stirring rod (46) is also provided on the pneumatic drive component (45). High-pressure nitrogen in the gas storage tank (3) is input into the chamber (41) through the first air pipe (42) to drive the pneumatic drive component (45) and the stirring rod (46) to operate, thereby stirring the anaerobic sludge to ensure the sludge is homogeneous, and outputting the high-pressure nitrogen from the output port (43) to aerate the anaerobic sludge sample in the storage box (2).

4. The automatic sampling device for anaerobic biological toxicity evaluation of industrial wastewater according to claim 3, characterized in that: The pneumatic driving member (45) comprises: a first gear (451) rotatably connected to the inner side of the chamber (41); A shaft (452) is rotatably connected to the inner side of the chamber (41) and one end thereof penetrates and extends to the outside of the chamber (41), and an end of the stirring rod (46) is fixedly connected to an end of the shaft (452) located outside the chamber (41); The second gear (453) is fixedly mounted on the outer side of the shaft (452) and meshes with the first gear (451).

5. The automatic sampling device for anaerobic biological toxicity evaluation of industrial wastewater according to claim 2, characterized in that: The sample supply assembly (5) comprises: A pump body (51) is fixedly mounted on the outside of the storage box (2), and a suction end of the pump body (51) is connected to a suction pipe (52) having one end penetrating and extending into the interior of the storage box (2). The number of the pump body (51) and the suction pipe (52) are both two, and the two suction pipes (52) are respectively arranged in the anaerobic sludge sample storage area and the nutrient solution storage area; A communication seat (53) is provided on the output end of the pump body (51) and is in communication with the output end of the pump body (51); A filling pipe (54), one end of which is connected to the connecting seat (53), the number of the filling pipes (54) is not less than eight and is equally divided into two groups, respectively arranged on the two connecting seats (53), the outer sides of two adjacent filling pipes (54) are fixedly connected to the rubber plugs (55), the number of the rubber plugs (55) is four, and each rubber plug (55) is provided with two filling pipes (54) respectively from the two connecting seats (53); The flow valves (56) are fixedly installed on the outside of the filling pipe (54), and the number and distribution positions of the flow valves (56) are matched one by one with the filling pipe (54).

6. The automatic sampling device for anaerobic biological toxicity evaluation of industrial wastewater according to claim 5, characterized in that: The gas drive assembly (6) comprises: A main air outlet pipe (61) is fixedly installed on the outside of the storage box (2) and is in communication with the anaerobic sludge sample storage area; A piston cylinder (62) is fixedly mounted on the outside of the storage box (2). A piston rod (63) is provided on the inside of the piston cylinder (62) with one end penetrating and extending to the outside of the piston cylinder (62). The piston rod (63) can be telescopically displaced along the inside of the piston cylinder (62). There are two piston cylinders (62), and the two piston cylinders (62) are connected to each other through a connecting pipe. An elastic member (64), one end of which is fixedly mounted on the inner side of the piston cylinder (62), and the other end of which is fixedly connected to the end of the piston rod (63); a second air pipe (65), one end of which is in communication with the main air outlet pipe (61) and the other end of which is in communication with the piston cylinder (62); a second solenoid valve (66) is further provided on the outside of the second air pipe (65); a deflation solenoid valve (67), which is disposed on the piston cylinder (62) and is in communication with the piston cylinder (62); The mounting plate (68) is fixedly mounted on one end portion of the piston rod (63) outside the piston cylinder (62), and the rubber plug (55) and the mounting plate (68) are fixedly connected to each other.

7. The automatic sampling device for anaerobic biological toxicity evaluation of industrial wastewater according to claim 6, characterized in that: The pipeline communication component (7) comprises: A third air outlet pipe (71), one end of which is in communication with the main air outlet pipe (61), and the other end of which passes through and extends into one of the communication seats (53); a third solenoid valve (72) is provided on the third air outlet pipe (71); The intercommunication pipe (73) is connected between the two communication seats (53), and a fourth electromagnetic valve (74) is provided on the outside of the intercommunication pipe (73).

8. The automatic sampling device for anaerobic biological toxicity evaluation of industrial wastewater according to claim 7, characterized in that: An output tube (10) is also provided through the rubber stopper (55), and the other end of the output tube (10) is connected to a gas quantity measuring component (11).

9. The automatic sampling device for anaerobic biological toxicity evaluation of industrial wastewater according to claim 2, characterized in that: The gas storage tank (3) is also provided with a gas filling port (12).

10. The method of the automatic sampling device for anaerobic biological toxicity evaluation of industrial wastewater according to any one of claims 1 to 9, characterized in that: The following steps are involved: S10, adding anaerobic sludge sample and nutrient solution to the anaerobic sludge sample storage area and nutrient solution storage area in the storage box (2) through the feeding pipe (9), opening the first solenoid valve (44), and inputting the high-pressure nitrogen in the gas storage tank (3) into the chamber (41) of the nitrogen delivery assembly (4) through the first air pipe (42), driving the first gear (451) and the second gear (453) of the pneumatic driving member (45) to engage with each other, driving the shaft (452) to rotate axially, causing the stirring rod (46) to stir the anaerobic sludge to ensure the sludge is homogeneous, and at the same time, the nitrogen is output from the output port (43) of the chamber (41) to aerate the anaerobic sludge sample in the storage box (2); S20, opening the second solenoid valve (66) of the gas drive assembly (6), outputting the nitrogen in the anaerobic sludge sample storage area to the inner side of the piston cylinder (62) through the main gas outlet pipe (61) and the second gas pipe (65), and pushing the piston rod (63) outward under the action of gas pressure, placing the conical flask (1) directly below the rubber stopper (55), and after the piston rod (63) is fully extended, closing the second solenoid valve (66) and the air release solenoid valve (67); S30, opening the venting solenoid valve (67), the gas in the piston cylinder (62) is discharged, and the rubber stopper (55) is buckled downward at the opening of the conical flask (1) under the action of the elastic member (64); S40, opening the third solenoid valve (72) and the fourth solenoid valve (74) of the pipeline connecting assembly (7), and outputting the nitrogen in the anaerobic sludge sample storage area to the inner side of one of the connecting seats (53) through the main outlet pipe (61) and the third outlet pipe (71), and filling the other connecting seat (53) with nitrogen through the intercommunication pipe (73), and injecting the nitrogen from the connecting seat (53) and the filling pipe (54) into the conical flask (1), replacing the air in the connecting seat (53), the filling pipe (54) and the conical flask (1) with nitrogen. In this process, the gas amount measuring assembly (11) connected to the output pipe (10) on the rubber stopper (55) measures whether the air is completely replaced; S50, the pump body (51) and the flow valve (56) of the sample supply assembly (5) are opened, and the two pump bodies (51) respectively suck the anaerobic sludge sample and the nutrient solution from the anaerobic sludge sample storage area and the nutrient solution storage area of ​​the storage box (2) through the suction pipe (52), and inject them into the conical flask (1) through the connecting seat (53) and the filling pipe (54), thereby completing the automatic sampling of samples for anaerobic toxicity evaluation of industrial wastewater.

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