Serum-free, animal-source-component-free and DMSO-free cell freezing medium as well as preparation method and application thereof

By preparing a serum-free, animal-derived, and DMSO-free cell freezing solution containing components such as lactobionic acid, the toxicity and ethical issues of traditional freezing solutions are resolved, and a freezing effect with high survival rate and function retention is achieved, which is suitable for a variety of cell types.

CN120678083APending Publication Date: 2025-09-23IPHASE THERAPEUTICS LTD
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Patent Information

Application Number
CN202510713751.6
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-05-30
Publication Date
2025-09-23

AI Technical Summary

Technical Problem

Existing technologies are unable to provide a safe, efficient, and completely serum-free, animal component-free, and DMSO-free cell freezing solution, and are unable to meet the high standards required in fields such as regenerative medicine, cell therapy, and biopharmaceuticals.

Method used

The cryopreservation solution consists of components such as lactobionic acid, raffinose, hydroxyethyl starch, potassium dihydrogen phosphate, magnesium sulfate, glutathione, allopurinol, adenosine, insulin, trehalose and proline. The serum-free, animal-derived component-free and DMSO-free cell cryopreservation solution is prepared through a specific process, providing multi-mechanism synergistic cell protection.

Benefits of technology

It achieves high survival rate and function retention, is suitable for a variety of cell types, has a survival rate of ≥90% after cryopreservation, and has no significant decline in cell function, avoiding the toxicity and ethical issues of traditional cryopreservation fluids.

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Abstract

The invention relates to a serum-free, animal-source-component-free and DMSO-free cell freezing medium and a preparation method and application thereof, and relates to the technical field of cell and biological engineering, and the serum-free, animal-source-component-free and DMSO-free cell freezing medium comprises the following components (according to the concentration range): 10-50mM of lacturonic acid; 50 to 200 mM of raffinose; 2-8% (w / v) of hydroxyethyl starch; 1 to 5 mM of monopotassium phosphate; 0.5 to 2 mM of magnesium sulfate; 1 to 5 mM of glutathione; allopurinol: 0.1 to 1 mM; 0.5 to 2 mM of adenosine; insulin: 0.1 to 1 [mu] g / mL; 50 to 150 mM of trehalose; proline: 20 to 100 mM; 10-20% (v / v) of glycerol; and a pH buffer. The application does not contain serum, animal-derived substances and DMSO, and avoids the problems of toxicity, immunogenicity and ethics.
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