Immune cell serum-free cryopreservation liquid and preparation method thereof

Abstract

The invention relates to an immune cell serum-free cryopreservation solution and a preparation method thereof, and belongs to the technical field of cell biology and biological pharmacy. According to the cryopreservation liquid, DMSO and DPBS are mixed according to the volume ratio of 10: 90 to form a buffer solution, hydroxyethyl starch, heparin sodium, trehalose, glutamine, HEMA and taurine are added into the buffer solution, a serum-free cryopreservation formula with definite components and high stability is formed, and the preparation method comprises the five steps of accurate component weighing, buffer solution preparation, dissolution and uniform mixing, constant volume and pH adjustment and sterilization and filtration. Wherein the component concentration can be adjusted within a preset interval according to experimental requirements, the cryopreservation liquid is suitable for immune cell preservation experiments under different conditions, the cryopreservation liquid does not contain serum components, the heterologous protein pollution risk can be remarkably reduced, and the survival rate and the biological function retention rate of cryopreserved cells are increased. The cell cryopreservation device is suitable for medium and long term cryopreservation of various immune cells such as CAR-T cells and NK cells, and has good biological safety and clinical application prospects.