Method for optimizing preparation process of aralia elata polysaccharide oral liquid based on response surface method
The preparation process of Aralia dasyphylla polysaccharide oral liquid was optimized by response surface methodology, which solved the problems of insufficient clarity and active ingredient retention in the existing technology, achieved efficient and stable preparation of polysaccharide oral liquid, and promoted efficient utilization of resources and consistency of product quality.
Patent Information
- Application Number
- CN202511006661.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-07-22
- Publication Date
- 2025-09-26
AI Technical Summary
The existing technology makes it difficult to meet the dual requirements of clarity and active ingredients in the preparation of red Aralia polysaccharide oral liquid, and lacks standardized and refined preparation processes, resulting in underutilization of resources and insufficient product stability.
The response surface methodology was used to optimize the preparation process of Aralia dasyphylla polysaccharide oral liquid. Through water bath heating extraction, alcohol precipitation and clarifier treatment, combined with a multi-factor interaction model of preservatives, stabilizers and pH value, the retention of polysaccharide active ingredients and product stability were ensured.
The efficient preparation of red Aralia polysaccharide oral liquid was achieved, the clarity of the product and the polysaccharide retention rate were improved, the controllability and stability of industrial production were provided, and the efficient utilization of resources and the consistency of product quality were promoted.
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Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of Aralia dasyphylla polysaccharide utilization, and in particular relates to a method for optimizing the preparation process of Aralia dasyphylla polysaccharide oral liquid based on response surface methodology. Background Art
[0002] Aralia rubra ( Aralia echinocaulis Aralia aralia is a deciduous shrub or small tree of the genus Aralia in the Araliaceae family. It has a long history of medicinal use, documented in various Compendium of Materia Medica. Its rhizome and bark are both medicinal, warm in nature and slightly bitter in taste, with benefits such as dispelling wind and dampness, promoting blood circulation and unblocking meridians, and reducing swelling and detoxifying. Studies have shown that Aralia aralia contains a variety of chemical components, such as triterpenoid saponins, flavonoids, polysaccharides, and volatile oils, with triterpenoid saponins and polysaccharides being the primary chemical components. Aralia aralia polysaccharides are used to treat rheumatoid arthritis, and oral liquid forms are under development. Currently, no research has been conducted on the preparation of an oral liquid of Aralia aralia polysaccharide.
[0003] Currently, orthogonal experiments are often used to optimize the process and sensory evaluation of polysaccharide oral liquids. However, orthogonal experiments struggle to accurately capture the interactions between multiple factors, and often focus on a single dimension when selecting indicators. This makes it difficult to balance the dual requirements of clarity and active ingredient retention in the preparation of Aralia dasyphylla polysaccharide oral liquids, resulting in limitations in the preparation of Aralia dasyphylla polysaccharide oral liquids. The dried rhizome resources of Aralia dasyphylla are underdeveloped, and the high added value of their polysaccharide components has not been effectively converted, resulting in a lack of standardized and refined preparation processes. As a dual-purpose medicinal and edible plant, Aralia dasyphylla's polysaccharide components possess natural active properties. Transforming these into safe, edible, and convenient oral liquid preparations, while maintaining their efficacy while improving product stability and applicability, has become a key issue that needs to be addressed. Summary of the Invention
[0004] In view of the above problems, the present invention discloses a method for optimizing the preparation process of Aralia dasyphylla polysaccharide oral liquid based on response surface methodology, comprising the following steps: Weigh the dried rhizomes of Aralia dasyphylla, add distilled water and perform water bath heating extraction, combine the filtrates and concentrate to obtain a concentrate; Anhydrous ethanol is added to the concentrated solution for alcohol precipitation, the polysaccharide after alcohol precipitation is filtered to obtain flocculent precipitate, and then dried to obtain red Aralia polysaccharide precipitate; The red Aralia polysaccharide was precipitated to form a red Aralia polysaccharide solution, chitosan-acetic acid or diatomaceous earth was added as a clarifier to the red Aralia polysaccharide solution, and the optimal clarifier and dosage of the red Aralia polysaccharide solution were determined using light transmittance and polysaccharide retention rate as indicators. A single factor experiment was conducted with preservatives, stabilizers, and pH values as variables and total polysaccharide content as an indicator to screen out the level ranges of preservatives, stabilizers, and pH values that affect total polysaccharide content. A response surface experiment was conducted with the level ranges of screened preservatives, stabilizers, and pH values as factor levels, and the total polysaccharide content in the Aralia dasyphylla polysaccharide solution as an indicator. Based on the results of the response surface experiment, the optimal preparation process parameters of the Aralia dasyphylla polysaccharide oral liquid were determined.
[0005] Furthermore, the mass volume ratio of the dried rhizome of Aralia dasyphylla to distilled water is 1:10-15 g / mL, and illustratively, can be 1:10, 1:12, or 1:15 g / mL.
[0006] Furthermore, the water bath heating extraction time is 2-4 hours, and the number of extractions is 1-3 times. Exemplarily, it can be 2, 2.5, 3, or 4 hours, and the number of extractions in advance is 1, 2, or 3 times.
[0007] Furthermore, anhydrous ethanol is added to the concentrate until the volume fraction of ethanol in the concentrate is 75-85% for alcohol precipitation, and the alcohol precipitation time is 12-36 hours; illustratively, it can be 12, 15, 18, 20, 24, 30, or 36 hours.
[0008] Furthermore, the amount of chitosan-acetic acid used ranges from 0.05 to 0.6% of the volume of the Aralia dasyphylla polysaccharide solution.
[0009] Furthermore, the dosage of the diatomaceous earth ranges from 1 to 9% of the volume of the Aralia dasyphylla polysaccharide solution.
[0010] Furthermore, the preservative is ethyl hydroxybenzoate, and the dosage range is 0.2-0.4% of the volume of the Aralia dasyphylla polysaccharide solution.
[0011] Furthermore, the stabilizer is polyvinyl pyrrolidone, and the amount range is 0.2-0.4% of the volume of the Aralia dasyphylla polysaccharide solution.
[0012] Furthermore, the pH value is 4-5.
[0013] In a second aspect, the present invention proposes an oral liquid of red Aralia polysaccharide, which is prepared by the method for optimizing the preparation process of red Aralia polysaccharide oral liquid based on response surface methodology. The oral liquid of red Aralia polysaccharide comprises the following raw materials in volume percentage: 0.03-0.05% ethyl hydroxybenzoate, 0.20-0.30% polyvinyl pyrrolidone and the remainder red Aralia polysaccharide solution.
[0014] Beneficial effects of the present invention: The present invention utilizes response surface analysis to optimize the preparation process of red aralia polysaccharide oral liquid, and uses transmittance and polysaccharide retention rate as dual core indicators to screen the optimal solution of the clarifier. While ensuring clarity, the active ingredients of red aralia polysaccharide are retained to the greatest extent, avoiding excessive loss of polysaccharide due to improper selection of clarifier, and ensuring the product's efficacy material basis and quality stability. In addition, with preservatives, stabilizers, and pH values as variables, a multi-factor interaction model is constructed with the help of the response surface method, breaking through the limitations of single-factor experiments, significantly improving the controllability and scientificity of the preparation process, and being able to effectively seek the optimal combination conditions of red aralia polysaccharide oral liquid, providing accurate and stable technical reference for industrial production, reducing trial and error costs, and improving production efficiency and product quality consistency.
[0015] The present invention provides a feasible path for the development of red Aralia resources through a standardized and refined oral liquid preparation process, converts the underutilized dried rhizomes of red Aralia into high-value-added polysaccharide oral liquid products, effectively taps the potential of natural plant resources, and promotes the rational circulation and efficient utilization of resources; compared with the risk of toxic and side effects of Western medicine in the treatment of rheumatoid arthritis, red Aralia, as a dual-purpose plant for medicine and food, its polysaccharide oral liquid, when exerting its medicinal value, has the advantages of natural ingredients, is safe and edible, and does not need to worry about the accumulation of drug toxicity when taken for a long time or for daily conditioning. It can not only assist in improving rheumatoid arthritis and other diseases with the help of the active ingredients of red Aralia polysaccharide, but also be integrated into health management as a medicine and food product.
[0016] Other features and advantages of the present invention will be described in the following description, and in part will become apparent from the description, or will be understood by practicing the present invention. The purpose and other advantages of the present invention can be realized and obtained by the structures pointed out in the description, claims and drawings. BRIEF DESCRIPTION OF THE DRAWINGS
[0017] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following is a brief introduction to the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.
[0018] Figure 1 A schematic diagram showing the effect of the clarifier chitosan-acetic acid on the retention rate of Aralia dasyphylla polysaccharide and the light transmittance of the solution in an embodiment of the present invention is shown; Figure 2 A schematic diagram showing the effect of diatomaceous earth on the retention rate of Aralia rubra polysaccharide and the light transmittance of the solution in an embodiment of the present invention is shown; Figure 3 A schematic diagram showing the effect of ethylparaben on the total polysaccharide content of Aralia rubra in an embodiment of the present invention is shown; Figure 4 A schematic diagram showing the effect of polyvinyl pyrrolidone on the total polysaccharide content of Aralia rubra in an embodiment of the present invention is shown; Figure 5 A schematic diagram showing the effect of pH regulator on the total polysaccharide content of Aralia rubra in an embodiment of the present invention is shown; Figure 6 The response surface (left) and contour line (right) diagrams of the effects of ethyl hydroxybenzoate and polyvinyl pyrrolidone on the total polysaccharide content in the embodiments of the present invention are shown; Figure 7 The response surface (left) and contour line (right) diagrams of the effects of ethyl paraben and pH regulator on the total polysaccharide content in the embodiments of the present invention are shown; Figure 8 The response surface (left) and contour line (right) diagrams of the effects of pH regulator and polyvinyl pyrrolidone on the total polysaccharide content in the examples of the present invention are shown. DETAILED DESCRIPTION
[0019] To make the objectives, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts shall fall within the scope of protection of the present invention.
[0020] Example 1 The extraction of crude polysaccharide from Aralia rubra includes the following steps: Step 1: Weigh an appropriate amount of dried rhizome of Aralia dasyphylla, add 10 times the volume of distilled water, heat in a 100°C water bath for 3 hours, repeat the extraction twice, combine the filtrates, filter while hot, and then concentrate using a rotary evaporator; Step 2: adding anhydrous ethanol to the concentrate until the volume fraction of ethanol in the concentrate is 80%, standing for 24 hours for alcohol precipitation, filtering the polysaccharide after alcohol precipitation to obtain a flocculent precipitate, and drying to obtain the red Aralia polysaccharide precipitate.
[0021] Example 2 Preparation of Aralia dasyphylla polysaccharide solution: Accurately weigh 0.5 g of Aralia dasyphylla polysaccharide precipitate, add distilled water to a 250 mL volumetric flask to obtain Aralia dasyphylla polysaccharide solution.
[0022] Preparation of chitosan-acetic acid solution: Accurately weigh 0.1 g of chitosan into a 25 mL beaker, add 10 mL of 1% acetic acid solution into the beaker, stir thoroughly, let it stand for 24 hours, and set aside.
[0023] Preparation of diatomaceous earth: Accurately weigh 4 g of diatomaceous earth into a 500 mL beaker, add 196 mL of distilled water to the beaker, stir thoroughly, let stand for 12 hours, and set aside.
[0024] Chitosan-acetic acid (0.05%, 0.15%, 0.3%, 0.45%, 0.6% by volume of the Aralia chinensis polysaccharide solution) and diatomaceous earth (1%, 3%, 5%, 7%, 9% by volume of the Aralia chinensis polysaccharide solution) were added to the Aralia chinensis polysaccharide solution, respectively.
[0025] The prepared red Aralia polysaccharide solution was measured, 30 mL as a group, and divided into 5 groups. The clarifiers of the corresponding concentrations in Table 1 were added to the red Aralia polysaccharide solution. The clarifiers were slowly added while stirring continuously with a glass rod. The solution was placed in a 30°C water bath for 0.5 h, cooled to room temperature, allowed to stand for 12 h, and centrifuged for 30 min. The supernatant was taken and the transmittance and polysaccharide retention rate were comprehensively considered as indicators to select the optimal clarifier and the optimal dosage.
[0026] Table 1 Clarifying agent type and dosage
[0027] The Aralia chinensis polysaccharide solution was clarified using chitosan-acetic acid and diatomaceous earth at different concentrations as shown in Table 1. After clarification, the total polysaccharide content in the Aralia chinensis polysaccharide solution was determined according to the following method. The determination method is as follows: (1) Accurately weigh 0.04 g of anthrone reagent into a beaker, slowly add 40 mL of 80% sulfuric acid along the wall of the beaker to dissolve it, transfer it to a brown reagent bottle to obtain anthrone sulfuric acid reagent, and store it in the dark; (2) Accurately weigh 10 mg of glucose standard dried to constant weight at 105°C, dissolve it in distilled water, transfer it to a 100 mL volumetric flask, make up to volume, shake well, and prepare a glucose standard solution with a concentration of 0.1 mg / mL; (3) Accurately pipette 0, 0.20, 0.40, 0.60, 0.80, and 1.00 mL of glucose standard solution into a stoppered test tube, dilute to 1 mL with distilled water, quickly add 4 mL of the prepared anthrone sulfuric acid reagent in an ice bath, shake well, heat in a boiling water bath for 10 min, remove, cool to room temperature, and measure the absorbance at a wavelength of 620 nm. (4) With the glucose concentration as the horizontal coordinate x and the absorbance as the vertical coordinate y, the regression equation is y=8.6063x+0.051, R 2 =0.9967, linear range 0.021˜0.105 mg / mL; (5) The absorbance of the Aralia chinensis polysaccharide solution was measured and substituted into the regression equation to calculate the glucose concentration (i.e., polysaccharide concentration) in the Aralia chinensis polysaccharide solution. The total polysaccharide content was calculated according to the following formula.
[0028]
[0029] Wherein, C is the polysaccharide concentration in the Aralia dasyphylla polysaccharide sample to be tested (mg / mL); V is the fixed volume of the Aralia dasyphylla polysaccharide sample extract (mL); and m is the mass of the Aralia dasyphylla polysaccharide sample (mg).
[0030] The calculation formula of polysaccharide retention rate is as follows:
[0031] Wherein, m1 is the total content of Aralia dasyphylla polysaccharide before adding the clarifier, and m2 is the total content of Aralia dasyphylla polysaccharide after adding the clarifier, and the unit is mg.
[0032] The method for determining the transmittance is as follows: add a clarifier to the Aralia rubra polysaccharide solution without adding anthrone sulfuric acid, measure the absorbance at 620 nm, and calculate the transmittance according to the following conversion formula: A=-logT Wherein, A is the absorbance of the Aralia chinensis polysaccharide solution, and T is the transmittance of the Aralia chinensis polysaccharide solution.
[0033] The results of light transmittance and polysaccharide retention of Aralia dasyphylla polysaccharide solution clarified by chitosan-acetic acid and diatomaceous earth are shown in Figure 2. Figure 1 and Figure 2 As shown, as the chitosan-acetic acid dosage increases, light transmittance first increases and then decreases. At a dosage of around 0.2%, light transmittance reaches a relatively high level, indicating that the clarification effect at this point makes the solution transparent and impurities are relatively fully removed. The polysaccharide retention rate decreases with increasing chitosan-acetic acid dosage, meaning that a higher dosage leads to greater polysaccharide loss due to interactions with the clarifier and other factors. It increases with increasing diatomaceous earth dosage, indicating that increasing dosage helps improve solution light transmittance and remove more impurities. The polysaccharide retention rate initially remains relatively stable with increasing diatomaceous earth dosage, then rapidly decreases, indicating that diatomaceous earth usage beyond a certain level results in significant polysaccharide loss.
[0034] Example 3 Single factor experiment: set the dosage of preservative ethylparaben (0.01%, 0.02%, 0.03%, 0.04%, 0.05%), the dosage of stabilizer polyvinylpyrrolidone (0.1%, 0.2%, 0.3%, 0.4%, 0.5%), and pH value (4, 5, 6, 7, 8) for three single factors. Add the above additives to the polysaccharide solution, stir and dissolve them, and add them to the polysaccharide solution. Calculate the total polysaccharide content of Aralia dasyphylla polysaccharide under different variable parameters. The determination method of total polysaccharide content is as follows: (1) Accurately weigh 0.04 g of anthrone reagent into a beaker, slowly add 40 mL of 80% sulfuric acid along the wall of the beaker to dissolve it, transfer it to a brown reagent bottle to obtain anthrone sulfuric acid reagent, and store it in the dark; (2) Accurately weigh 10 mg of glucose standard dried to constant weight at 105°C, dissolve it in distilled water, transfer it to a 100 mL volumetric flask, make up to volume, shake well, and prepare a glucose standard solution with a concentration of 0.1 mg / mL; (3) Accurately pipette 0, 0.20, 0.40, 0.60, 0.80, and 1.00 mL of glucose standard solution into a stoppered test tube, dilute to 1 mL with distilled water, quickly add 4 mL of the prepared anthrone sulfuric acid reagent in an ice bath, shake well, heat in a boiling water bath for 10 min, remove, cool to room temperature, and measure the absorbance at a wavelength of 620 nm. (4) With the glucose concentration as the horizontal coordinate x and the absorbance as the vertical coordinate y, the regression equation is y=8.6063x+0.051, R 2 =0.9967, linear range 0.021˜0.105 mg / mL.
[0035] The specific process of the single-factor experiment is as follows: (1) Effects of different amounts of ethyl hydroxybenzoate on the total polysaccharide content of Aralia dasyphylla: Accurately weigh 0.1g of crude polysaccharide from Aralia rubra and dilute it to a 250mL volumetric flask. Take 30mL of each, fix the amount of polyvinylpyrrolidone added at 0.1%, the pH value at 5, add different amounts of ethyl hydroxybenzoate (0.01%, 0.02%, 0.03%, 0.04%, 0.05%), stir until dissolved, and calculate the total polysaccharide content according to the above steps. The results are as follows: Figure 3 As shown, it can be seen that when the concentration of ethyl paraben increases, the total polysaccharide content first increases and then decreases, and the optimal concentration is 0.03%.
[0036] (2) Effect of different amounts of polyvinylpyrrolidone on the total polysaccharide content; Accurately weigh 0.1g of crude polysaccharide from Aralia chinensis, dilute to a 250mL volumetric flask, take 30mL of each, fix the amount of ethylparaben added at 0.01%, the pH value at 5, add different amounts of polyvinylpyrrolidone (0.1%, 0.2%, 0.3%, 0.4%, 0.5%), stir until dissolved, and calculate the total polysaccharide content of Aralia chinensis according to the above steps. The results are as follows: Figure 4 As shown, it can be seen that when the concentration of polyvinyl pyrrolidone increases, the total polysaccharide content first increases and then decreases, and the optimal concentration is 0.3%.
[0037] (3) Effects of different pH values on the total polysaccharide content of Aralia rubra; Accurately weigh 0.1g of crude polysaccharide from Aralia rubra, and dilute it to a 250mL volumetric flask. Take 30mL of each, fix the amount of ethylparaben added at 0.01%, the amount of polyvinylpyrrolidone added at 0.1%, add different amounts of pH regulator (4, 5, 6, 7, 8), stir until dissolved, and calculate the total polysaccharide content according to the above steps. The results are as follows: Figure 5 As shown, it can be seen that the optimal pH of the solution is around 5.
[0038] Example 4 Response surface experiment: Using Aralia rubra extract as the main raw material, A: preservative (ethyl hydroxybenzoate), B: stabilizer (polyvinyl pyrrolidone), C: pH value as factors, and Aralia rubra total polysaccharide content as the reference value, a response surface experiment with three factors and three levels was designed. The factor levels are shown in Table 2: Table 2 Factor levels
[0039] The three-factor three-level experimental design was carried out using Design-Expert11 software according to the Box-Benhnken design principle. The experimental scheme and response surface results are shown in Table 3.
[0040] Table 3 Response surface experimental design and results
[0041] The experimental data were subjected to multiple regression fitting using Desgin-Expert11 software, and the quadratic polynomial regression equation of the total polysaccharide content Y of Aralia rubra was obtained: Y=6.84-0.0388A-0.0600B+0.2138C-1.08AB-0.4825AC+0.8000BC+0.7888A 2 +0.4412B 2 +0.6037C 2 , R 2 =0.9822.
[0042] The results of variance analysis are shown in Table 4.
[0043] Table 4 Results of variance analysis of regression equation
[0044] In Table 4, P < 0.01 indicates an extremely significant difference. P<0.05 was considered significant, and P>0.05 was considered no significant difference.
[0045] According to the analysis of Desgin-Expert11 software, the effects of any two factors interacting on the total polysaccharide content of Aralia dasyphylla were analyzed and compared, and response surface diagrams and contour maps were drawn, such as Figure 6-Figure 8 The larger the slope of the response surface, the more sensitive the response value is to changes in the influencing factors.
[0046] Using Desgin-Expert 11 software and the results of a single-factor experiment, a mathematical model was established to analyze and fit the optimal conditions for the total polysaccharide content of Aralia dasyphylla: 0.04% ethyl hydroxybenzoate, 0.202% polyvinyl pyrrolidone, and a pH of 4.633. Under these conditions, the total polysaccharide content of Aralia dasyphylla was 9.601%. Based on actual operating conditions, the final optimized conditions were determined to be 0.04% ethyl hydroxybenzoate, 0.2% polyvinyl pyrrolidone, and a pH of 4. Verification of these conditions yielded a total polysaccharide content of 9.41%, very close to the predicted value of 9.601%. Three parallel experiments were conducted using this optimal preparation process, and the resulting products were all clear and free of precipitation, demonstrating that the optimized Aralia dasyphylla polysaccharide oral solution was well prepared.
[0047] Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein; and these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims
1. A method for optimizing the preparation process of Aralia dasyphylla polysaccharide oral liquid based on response surface methodology, characterized in that: The following steps are involved: Weigh the dried rhizomes of Aralia dasyphylla, add distilled water and perform water bath heating extraction, combine the filtrates and concentrate to obtain a concentrate; Anhydrous ethanol is added to the concentrated solution for alcohol precipitation, the polysaccharide after alcohol precipitation is filtered to obtain a flocculent precipitate, and the polysaccharide precipitate is obtained after drying; The red Aralia polysaccharide was precipitated to form a red Aralia polysaccharide solution, chitosan-acetic acid or diatomaceous earth was added as a clarifier to the red Aralia polysaccharide solution, and the optimal clarifier and dosage of the red Aralia polysaccharide solution were determined using light transmittance and polysaccharide retention rate as indicators. A single factor experiment was conducted with preservatives, stabilizers, and pH values as variables and total polysaccharide content as an indicator to screen out the level ranges of preservatives, stabilizers, and pH values that affect total polysaccharide content. A response surface experiment was conducted with the level ranges of screened preservatives, stabilizers, and pH values as factor levels, and the total polysaccharide content in the Aralia dasyphylla polysaccharide solution as an indicator. Based on the results of the response surface experiment, the optimal preparation process parameters of the Aralia dasyphylla polysaccharide oral liquid were determined.
2. The method for optimizing the preparation process of Aralia dasyphylla polysaccharide oral liquid based on response surface methodology according to claim 1, characterized in that: The mass volume ratio of dried rhizome of Aralia dasyphylla to distilled water is 1:10-15 g / mL.
3. The method for optimizing the preparation process of Aralia dasyphylla polysaccharide oral liquid based on response surface methodology according to claim 1, characterized in that: The water bath heating extraction time is 2-4h, and the number of extractions is 1-3 times.
4. The method for optimizing the preparation process of Aralia dasyphylla polysaccharide oral liquid based on response surface methodology according to claim 1, characterized in that: Anhydrous ethanol is added to the concentrated solution until the volume fraction of ethanol in the concentrated solution is 75-85%, and alcohol precipitation is performed for 12-36 hours.
5. The method for optimizing the preparation process of Aralia dasyphylla polysaccharide oral liquid based on response surface methodology according to claim 1, characterized in that: The dosage of the chitosan-acetic acid is in the range of 0.05-0.6% of the volume of the Aralia dasyphylla polysaccharide solution.
6. The method for optimizing the preparation process of Aralia dasyphylla polysaccharide oral liquid based on response surface methodology according to claim 1, characterized in that: The dosage of the diatomaceous earth is in the range of 1 to 9% of the volume of the Aralia dasyphylla polysaccharide solution.
7. The method for optimizing the preparation process of Aralia dasyphylla polysaccharide oral liquid based on response surface methodology according to claim 1, characterized in that: The preservative is ethyl hydroxybenzoate, and the dosage range is 0.2-0.4% of the volume of the Aralia rubra polysaccharide solution.
8. The method for optimizing the preparation process of Aralia dasyphylla polysaccharide oral liquid based on response surface methodology according to claim 1, characterized in that: The stabilizer is polyvinyl pyrrolidone, and the amount thereof is in the range of 0.2-0.4% of the volume of the Aralia rubra polysaccharide solution.
9. The method for optimizing the preparation process of Aralia dasyphylla polysaccharide oral liquid based on response surface methodology according to claim 1, characterized in that: The pH value is 4-5.
10. An Aralia rubra polysaccharide oral liquid, characterized in that: The polysaccharide oral liquid is prepared by the method for optimizing the preparation process of the polysaccharide oral liquid based on the response surface methodology according to any one of claims 1 to 8.