Industrialized seedling raising system and method for aralis tissue culture seedlings

Abstract

The invention belongs to the technical field of plant tissue culture, and provides an industrial seedling culture system for aralis tissue culture seedlings to solve the problems that in the prior art, tissue culture seedling environment control lacks light-temperature coupling regulation, CO2 concentration regulation and control are not accurate, and response of execution equipment is lagged. The system comprises a culture container, an environment monitoring module, a PLC controller and an execution module, the environment monitoring module collects temperature, illumination intensity and CO2 concentration data in the culture container in real time, the PLC controller prestores a light-temperature coupling parameter group of different growth stages, the light-temperature coupling parameter group is defined as L = k * T + b, k is 80-120lx / DEG C, b is-2000 to-1600lx, and the execution module is connected with the PLC controller. Temperature control equipment and a light supplementing lamp set are dynamically adjusted through the model, and when the COconcentration is lower than 400 ppm, a COgas cylinder gas supply system is started. The system can accurately regulate and control the growth environment of the aralis tissue culture seedlings, improves the seedling culture efficiency and quality, and is suitable for large-scale factory seedling culture production.

Description

Technical Field

[0001] This invention relates to the field of seedling technology. More specifically, this invention relates to a system and method for the industrialized cultivation of Aralia elata tissue culture seedlings. Background Technology

[0002] Aralia elata is a plant with high economic value, and the industrialized production of its tissue culture seedlings is of great significance for large-scale cultivation. However, some technical problems still exist in the current industrialized seedling production process of Aralia elata tissue culture, affecting the efficiency and quality of seedling production.

[0003] First, the regulation of environmental parameters during tissue culture seedling cultivation often lacks a systematic approach. Temperature and light intensity are key factors affecting seedling growth, but current techniques typically control them as independent variables, neglecting their synergistic effect. Studies have shown that the temperature and light requirements of Aralia elata tissue culture seedlings change dynamically at different growth stages, and a certain coupling relationship exists between the two. However, a control method that can dynamically adjust the photothermal coupling relationship according to growth stages is currently lacking, making it difficult to achieve an optimal growth environment for the seedlings. This may not only slow down the growth rate of the seedlings but also affect their morphogenesis and physiological activity.

[0004] Secondly, existing environmental control systems typically employ simple threshold control strategies. For example, when temperature or light intensity exceeds a preset range, the actuators (such as temperature controllers or supplemental lighting) will switch on and off. This control method easily leads to large fluctuations in environmental parameters, making it difficult to maintain stable culture conditions. Especially in factory-scale seedling cultivation, the microenvironment within the culture container is easily affected by external disturbances (such as seasonal changes, equipment overheating, etc.), and simple threshold control is insufficient for precise adjustment. Furthermore, due to the lack of a quantitative model of the light-temperature coupling relationship, the adjustments made by the actuators are often lagging or excessive, further exacerbating environmental instability.

[0005] Third, the regulation of carbon dioxide (CO2) concentration is often insufficiently considered in existing systems. CO2 is an important substrate for plant photosynthesis, but in the closed culture containers of tissue culture seedlings, CO2 concentration is easily fluctuated due to the respiration and photosynthesis of the seedlings. Current technologies typically only supplement CO2 when the concentration falls below a certain threshold, or adjust it through periodic ventilation, lacking dynamic monitoring and precise control. Especially under high-density culture conditions, the seedlings' demand for CO2 increases, and simple ventilation or supplemental aeration methods may cause sudden changes in CO2 concentration, affecting the photosynthetic efficiency of the tissue culture seedlings. Furthermore, the synergistic effect of CO2 concentration with temperature and light has not been fully considered, further limiting the growth potential of tissue culture seedlings.

[0006] The main reasons for the above problems include: 1) There is a lack of systematic research on the light and temperature requirements of Aralia elata tissue culture seedlings at different growth stages, especially the lack of a quantitative model of the light and temperature coupling relationship; 2) Existing control strategies fail to integrate the dynamic relationship of multiple environmental parameters, resulting in a lack of coordination in the adjustment of the execution equipment; 3) The regulation of CO2 concentration is not linked with light and temperature conditions, making it difficult to achieve synergistic optimization of environmental parameters.

[0007] Furthermore, the mathematical model of the light-temperature coupling relationship needs to be established based on a large amount of experimental data, while the growth response of Aralia elata tissue culture seedlings has nonlinear characteristics, which increases the difficulty of modeling; real-time control of multiple environmental parameters requires high-precision sensors and fast-response execution devices, which places high demands on the hardware reliability of the system; the number of culture containers in factory seedling production is large, and how to achieve a balance between centralized control and distributed regulation is also a technical challenge.

[0008] Therefore, there is an urgent need for a factory-scale seedling system that can dynamically adjust the light-temperature coupling relationship according to the growth stage of Aralia elata tissue culture seedlings and achieve synergistic control of CO2 concentration, so as to improve the efficiency and consistency of seedling cultivation. Summary of the Invention

[0009] One object of the present invention is to solve at least the above-mentioned problems and to provide at least the advantages that will be described later.

[0010] Another objective of this invention is to provide a factory-scale seedling production system and method for Aralia elata tissue culture seedlings. The system can achieve precise control of the growth environment of Aralia elata tissue culture seedlings, improve seedling production efficiency and quality, and is suitable for large-scale factory seedling production.

[0011] To achieve these objectives and other advantages according to the present invention, a factory-scale seedling production system for Aralia elata tissue culture seedlings is provided, comprising: The culture container is a rectangular box. The environmental monitoring module includes a temperature sensor, a light intensity sensor, and a CO2 concentration sensor installed inside the culture container; The PLC controller has pre-stored a set of photothermal coupling parameters for different growth stages. The set of photothermal coupling parameters defines a linear relationship between the target temperature range and the target light intensity range: L = k×T + b, where L is the light intensity in lx, T is the temperature in °C, k is the slope coefficient ranging from 80 lx / °C to 120 lx / °C, and b is the intercept coefficient ranging from -2000 lx to -1600 lx. The execution module includes a temperature control device, a supplementary lighting unit, a CO2 gas cylinder supply system, and an exhaust device. The PLC controller is connected to the environmental monitoring module via a data acquisition unit and to the execution module via the Modbus-RTU communication protocol; the PLC controller performs the following operations: S1 receives real-time data from temperature sensor, light intensity sensor and CO2 concentration sensor; S2. Call the corresponding light and temperature coupling parameter group according to the current growth stage of the Aralia elata tissue culture seedling; S3. When the real-time temperature deviates from the target temperature range, send an adjustment command to the temperature control device; when the real-time light intensity deviates from the target light intensity range, send an adjustment command to the supplementary lighting group; when the real-time CO2 concentration is below 400ppm, send an opening command to the CO2 cylinder supply system; when the real-time CO2 concentration is above 600ppm, send an opening command to the exhaust device.

[0012] Preferably, the top of the culture container is provided with a first sliding groove along its length direction, a first sliding block is provided in the first sliding groove, a mounting base is fixedly connected to the first sliding block, and a second sliding groove is provided on the mounting base along the width direction of the culture container, a second sliding block is provided in the second sliding groove, and a mounting block is fixedly connected to the second sliding block; The supplementary lighting assembly includes an adjustable height bracket and a light source module. The fixed end of the bracket is fixedly connected to the mounting block, and the adjustable end of the bracket is connected to the light source module. The light source module includes a substrate, an LED array soldered on the substrate, and a light mixing lens. The LED array includes multiple red LEDs and multiple blue LEDs. The peak wavelength of the red LEDs is 660 nm ± 5 nm, and the peak wavelength of the blue LEDs is 450 nm ± 5 nm. The red LEDs and blue LEDs are arranged in a rectangular grid to form an LED array, with three red LEDs and one blue LED forming a basic unit. The spacing between the rectangular grids is 15~20 mm. The light mixing lens is connected to the substrate by a snap-fit. The light mixing lens is located below the LED array and at a distance of 5-8 mm from the LED array. The material of the light mixing lens is PMMA.

[0013] Preferably, the temperature control device includes a semiconductor cooling chip and a heating resistance wire; arranged sequentially from the inside to the outside on the outer surface of the culture container: A thermally conductive silicone grease layer, with a thickness of 0.1~0.2 mm, is directly coated onto the outer surface of the culture container. A copper heat-conducting plate is bolted to the outer wall of the culture container; The cold end face of the semiconductor cooling chip is bonded to the first area of ​​the outer surface of the copper heat-conducting plate by thermal grease. The heating resistance wire is wound at equal intervals on the alumina ceramic skeleton with a wire spacing of 1~2 mm. The heating resistance wire is attached to the second area of ​​the outer surface of the copper heat-conducting plate through thermal grease. The distance between the first area and the second area is 10~15 mm. A polyimide heat insulation film layer is used to cover the semiconductor and the heating resistance wire; The hot end of the semiconductor cooling chip extends to the outside of the polyimide insulation film and is connected to a heat dissipation fin assembly, the surface of which is covered with an axial fan.

[0014] Preferably, the supplementary lighting assembly is equipped with an adjustable reflective system, the adjustable reflective system comprising: A reflector, located 50 mm to 100 mm directly below the supplementary lighting group, is made of anodized aluminum with a reflectivity of 85% to 90%, a surface roughness Ra≤0.2 μm, and an initial tilt angle of 15° to 30°. An angle adjuster is installed on the side wall of the culture container. The angle adjuster includes a stepper motor, a planetary gearbox with a reduction ratio of 10:1, and a universal coupling. The input shaft of the planetary gearbox is connected to the output shaft of the stepper motor, and the output end of the planetary gearbox is connected to the reflector through the universal coupling. The light intensity feedback unit includes four four-quadrant photoelectric sensors, which are embedded in the four corners of the bottom of the culture container. The photosensitive surface of the four-quadrant photoelectric sensors is flush with the surface of the culture medium and 50 mm away from the side wall of the container. The drive end of the stepper motor is connected to the pulse output interface of the PLC controller via a shielded cable. The signal terminal of the photoelectric sensor is connected to the analog input interface of the PLC controller via an AD conversion module; The PLC controller performs the following steps: A1. Implement feedback adjustments, as detailed below: A11. Read the real-time light intensity values ​​of the four photoelectric sensors; A12. Calculate the uniformity of light intensity U = minimum light intensity value / maximum light intensity value; A13. When U < 0.85, determine the horizontal and vertical tilt adjustment of the reflector based on the light intensity distribution gradient between the sensors, and output a control signal to the angle adjuster to perform the angle adjustment. A2. Perform feedback adjustment every 300 seconds.

[0015] Preferably, the CO2 cylinder supply system includes: The gas source unit includes a CO2 high-pressure gas cylinder and a pressure reducing and stabilizing valve. The CO2 high-pressure gas cylinder is installed on an independent gas cylinder rack outside the culture container and is connected to the inlet of the pressure reducing and stabilizing valve through a high-pressure hose. The outlet of the pressure reducing and stabilizing valve is connected to the inlet of the mass flow controller through a polyurethane gas pipe. A flow control unit includes a mass flow controller and a solenoid valve. The inlet of the mass flow controller is connected to the outlet of the pressure reducing and stabilizing valve via an air pipe, and the outlet of the mass flow controller is connected to the inlet of the solenoid valve via an air pipe. A microporous diffuser is embedded in the center of the bottom of a culture container, with the diffusion surface flush with the bottom surface of the container. The inlet of the microporous diffuser is connected to the outlet of the solenoid valve via a silicone hose. The signal input terminal of the mass flow controller is connected to the DA output module of the PLC controller; the power supply line of the solenoid valve is connected to the DO output terminal of the PLC through a relay. The PLC controller performs the following operations: B1. Read the real-time value C of the CO2 concentration sensor, in ppm; B2. If C < 400 ppm, calculate the set flow rate Q = 0.02 × (450 - C), output a current signal to the mass flow controller through the DA output module, and output a high level at the DO output terminal to open the solenoid valve; if C > 600 ppm, output a low level at the DO output terminal to close the solenoid valve and start the exhaust device. B3. Perform steps B1 to B2 above once every 60 seconds.

[0016] Preferably, the temperature sensor is installed at 1 / 2 the height of the inner wall of the culture container, 50 mm to 100 mm away from the inner wall, and is covered with an aluminum radiation shield. The light intensity sensor is installed at the center of the top of the culture container, with the photosensitive surface facing vertically downwards, at a distance of 200 mm to 300 mm from the supplemental lighting group; The CO2 concentration sensor is installed 300 mm to 500 mm upstream of the bottom vent of the culture container, with the air inlet facing the airflow direction and 100 mm above the bottom.

[0017] This invention also provides a method for the industrialized cultivation of Aralia elata tissue culture seedlings, utilizing the aforementioned industrialized cultivation system for Aralia elata tissue culture seedlings, specifically including the following steps: S1. Real-time temperature data, real-time light intensity data, and real-time CO2 concentration data are collected in the culture container through the environmental monitoring module. S2. Based on the current growth stage of the Aralia elata tissue culture seedlings, the preset light-temperature coupling parameter set is called from the PLC controller. The light-temperature coupling parameter set includes the target temperature range, the target light intensity range, and the linear relationship L = k×T + b. S3. When the real-time temperature data exceeds the target temperature range, send a command to the temperature control device to bring the temperature back to the target temperature range. S4. When the real-time light intensity data exceeds the target light intensity range, send a command to the supplementary lighting group to make the light intensity return to the target light intensity range. S5. When the real-time CO2 concentration data is below 400 ppm, send an opening command to the CO2 cylinder supply system; S6. When the real-time CO2 concentration data is higher than 600 ppm, send an activation command to the exhaust system; S7. Repeat steps S1 to S6 every 120 minutes.

[0018] Preferably, the current growth stage includes the rooting stage; the light-temperature coupling parameter set of the rooting stage satisfies the following: the target temperature range is 22℃ to 26℃, the target light intensity range is 5000 lx to 7000 lx, the slope coefficient k is 100 lx / ℃, and the intercept coefficient b is -1800 lx.

[0019] The present invention has at least the following beneficial effects: First, by pre-storing light and temperature coupling parameters (L=k×T+b), the system dynamically matches light and temperature, ensuring that the two are always in the optimal combination state, thereby improving the photosynthetic efficiency of Aralia elata tissue culture seedlings and promoting balanced growth.

[0020] Secondly, based on real-time monitoring data, the system automatically replenishes gas when CO2 is below 400ppm and starts exhaust ventilation when it is above 600ppm, ensuring that the concentration remains stable within a suitable range and avoiding the negative impact of sudden concentration changes on tissue culture seedlings.

[0021] Third, adopting variable frequency temperature control, dimmable LEDs, and closed-loop CO2 control strategies reduces parameter fluctuations, ensures smooth transition of environmental conditions, and reduces energy consumption and mechanical losses caused by frequent equipment start-ups and shutdowns.

[0022] Fourth, the PLC controller switches the corresponding light and temperature parameter groups according to the development stage of the tissue culture seedlings to achieve precise control throughout the entire cycle, improve the uniformity and survival rate of seedlings, and is suitable for industrial mass production.

[0023] Fifth, modular control is achieved through the Modbus-RTU protocol, which facilitates centralized management of multiple culture containers and allows parameters to be adjusted according to the needs of different plants, thus expanding the scope of application.

[0024] Other advantages, objectives and features of the present invention will become apparent in part from the following description, and in part from those skilled in the art through study and practice of the invention. Detailed Implementation

[0025] The present invention will be further described in detail below with reference to embodiments, so that those skilled in the art can implement it based on the description.

[0026] This invention provides a factory-scale seedling production system for Aralia elata tissue culture seedlings, comprising: The culture container is a rectangular box. The environmental monitoring module includes a temperature sensor, a light intensity sensor, and a CO2 concentration sensor installed inside the culture container; The PLC controller has pre-stored a set of photothermal coupling parameters for different growth stages. The set of photothermal coupling parameters defines a linear relationship between the target temperature range and the target light intensity range: L = k×T + b, where L is the light intensity in lx, T is the temperature in °C, k is the slope coefficient ranging from 80 lx / °C to 120 lx / °C, and b is the intercept coefficient ranging from -2000 lx to -1600 lx. The execution module includes a temperature control device, a supplementary lighting unit, a CO2 gas cylinder supply system, and an exhaust device. The PLC controller is connected to the environmental monitoring module via a data acquisition unit and to the execution module via the Modbus-RTU communication protocol; the PLC controller performs the following operations: S1 receives real-time data from temperature sensor, light intensity sensor and CO2 concentration sensor; S2. Call the corresponding light and temperature coupling parameter group according to the current growth stage of the Aralia elata tissue culture seedling; S3. When the real-time temperature deviates from the target temperature range, send an adjustment command to the temperature control device; when the real-time light intensity deviates from the target light intensity range, send an adjustment command to the supplementary lighting group; when the real-time CO2 concentration is below 400ppm, send an opening command to the CO2 cylinder supply system; when the real-time CO2 concentration is above 600ppm, send an opening command to the exhaust device.

[0027] In the above technical solution, the culture container can be a rectangular box made of polycarbonate material, with dimensions of 60cm in length, 40cm in width, and 50cm in height, and a wall thickness of 5mm, providing good light transmittance and heat insulation. The box can be equipped with an openable transparent cover for easy observation and operation. The environmental monitoring module can use a digital temperature sensor with a measurement range of 0-50℃ and an accuracy of ±0.5℃; the light intensity sensor can be a silicon photovoltaic cell type sensor with a range of 0-10000lx and an accuracy of ±5%; the CO2 concentration sensor can be an infrared absorption type with a measurement range of 0-2000ppm and an accuracy of ±50ppm. These sensors can be evenly distributed inside the box to avoid local environmental monitoring deviations.

[0028] The PLC controller can be an industrial-grade programmable logic controller, supporting the Modbus-RTU communication protocol. Its built-in storage module can store multiple sets of photo-temperature coupling parameters. The slope coefficient k can be set to 90 lx / ℃, 100 lx / ℃, or 110 lx / ℃, and the intercept coefficient b can be set to -1800 lx, -1900 lx, or -2000 lx, with specific values ​​adjusted according to the Aralia elata species and growth stage. The temperature control device can use a semiconductor cooling chip combined with a PID temperature controller, with a temperature control range of 15-30℃ and an accuracy of ±0.3℃. The supplemental lighting can be full-spectrum LED plant growth lights, with an adjustable light intensity range of 0-8000 lx. The CO2 gas cylinder supply system can be equipped with a pressure reducing valve and a solenoid valve, with an adjustable flow rate range of 0.1-5 L / min. The exhaust system can use a low-noise axial flow fan with an air volume of 30 m³ / h.

[0029] During system operation, the PLC controller collects real-time environmental data from inside the chamber. When the temperature falls below the set lower limit, the temperature control equipment heats up; when it rises above the upper limit, cooling is activated. Light intensity is dynamically adjusted using the formula L=k×T+b to control LED brightness. When the CO2 concentration is below 400ppm, the solenoid valve opens to replenish gas; when it exceeds 600ppm, the fan starts to ventilate. The response time of each actuator is less than 10 seconds, ensuring that environmental parameters remain stable within the set range. This system enables precise control of the growth environment for Aralia elata tissue culture seedlings, improving seedling uniformity and survival rate.

[0030] In another technical solution, the top of the culture container is provided with a first sliding groove along its length direction, a first sliding block is provided in the first sliding groove, a mounting base is fixedly connected to the first sliding block, and a second sliding groove is provided on the mounting base along the width direction of the culture container, a second sliding block is provided in the second sliding groove, and a mounting block is fixedly connected to the second sliding block. The supplementary lighting assembly includes an adjustable height bracket and a light source module. The fixed end of the bracket is fixedly connected to the mounting block, and the adjustable end of the bracket is connected to the light source module. The light source module includes a substrate, an LED array soldered on the substrate, and a light mixing lens. The LED array includes multiple red LEDs and multiple blue LEDs. The peak wavelength of the red LEDs is 660 nm ± 5 nm, and the peak wavelength of the blue LEDs is 450 nm ± 5 nm. The red LEDs and blue LEDs are arranged in a rectangular grid to form an LED array, with three red LEDs and one blue LED forming a basic unit. The spacing between the rectangular grids is 15~20 mm. The light mixing lens is connected to the substrate by a snap-fit. The light mixing lens is located below the LED array and at a distance of 5-8 mm from the LED array. The material of the light mixing lens is PMMA.

[0031] In the above technical solution, the first slide groove at the top of the culture container can be set along the length direction, with a width of 10mm to 15mm and a depth of 5mm to 8mm. The first slider can be made of aluminum alloy, with dimensions matching the slide groove, and a sliding resistance coefficient controlled between 0.1 and 0.15. The mounting base can be fixed on the slider, made of stainless steel with a thickness of 3mm to 5mm. The second slide groove can be set along the width direction of the culture container, with the same width as the first slide groove, and has a second slider inside. The mounting block fixed on the slider can be made of ABS engineering plastic, with dimensions of 50mm × 50mm × 20mm. The bracket for the supplementary lighting assembly can be a telescopic aluminum alloy bracket with an adjustment range of 200mm to 500mm. The substrate of the light source module can be an aluminum substrate with dimensions of 100mm × 100mm × 1.5mm, and the surface is copper-plated to improve heat dissipation performance. In the LED array, red LEDs can be selected with a peak wavelength of 660nm±5nm, and blue LEDs can be selected with a peak wavelength of 450nm±5nm. The red and blue LEDs are arranged in a 3:1 ratio to form a rectangular grid, and the grid spacing can be set to 18mm. The mixing lens can be made of PMMA material with a thickness of 2mm to 3mm, and is fixed to the bottom of the substrate by clips, 6mm away from the LED array.

[0032] The installation position of the supplemental lighting unit can be adjusted according to the size of the culture container, typically located at the top center of the container, 300mm to 400mm from the surface of the culture medium. The power of the LED array can be set to 1W per red LED and 0.5W per blue LED, with an overall adjustable light intensity range of 1000lx to 8000lx. The design of the light mixing lens ensures uniform light distribution, avoiding localized excessively high or low light intensity. The adjustable end of the bracket allows for fine-tuning of the height via a threaded structure, with an adjustment accuracy of ±5mm. The connection between the mounting block and the bracket can be secured with bolts to ensure stability. The driving circuit of the LED array can be integrated on the substrate, adjusting brightness via a PWM signal with a response time of less than 100ms.

[0033] This implementation allows for flexible adjustment of the supplemental lighting and uniform light distribution, ensuring that Aralia elata tissue culture seedlings receive suitable light conditions at different growth stages. The specific ratio of red and blue LEDs meets the spectral requirements of the seedlings, promoting photosynthesis. The use of a light-mixing lens further improves light uniformity, preventing localized burns or insufficient light. The adjustable design of the support allows the light source position to be adjusted according to actual needs, adapting to the layout of different culture containers. The overall structure is simple, reliable, and easy to maintain and replace.

[0034] In another technical solution, the temperature control device includes a semiconductor cooling chip and a heating resistance wire; arranged sequentially from the inside to the outside on the outer surface of the culture container: A thermally conductive silicone grease layer, with a thickness of 0.1~0.2 mm, is directly coated onto the outer surface of the culture container. A copper heat-conducting plate is bolted to the outer wall of the culture container; The cold end face of the semiconductor cooling chip is bonded to the first area of ​​the outer surface of the copper heat-conducting plate by thermal grease. The heating resistance wire is wound at equal intervals on the alumina ceramic skeleton with a wire spacing of 1~2 mm. The heating resistance wire is attached to the second area of ​​the outer surface of the copper heat-conducting plate through thermal grease. The distance between the first area and the second area is 10~15 mm. A polyimide heat insulation film layer is used to cover the semiconductor and the heating resistance wire; The hot end of the semiconductor cooling chip extends to the outside of the polyimide insulation film and is connected to a heat dissipation fin assembly, the surface of which is covered with an axial fan.

[0035] In the above technical solution, the thermoelectric cooler of the temperature control device can be model TEC1-12706, with a cooling power of 60W, an operating voltage of 12V, and a cold end face size of 40mm×40mm. The heating resistance wire can be made of nickel-chromium alloy, with a diameter of 0.5mm and a resistance of 20Ω / m, wound on an alumina ceramic skeleton with a length of 200mm and an outer diameter of 10mm. The thermally conductive silicone grease layer can be model with a thermal conductivity of 3.5W / (m·K) and a coating thickness of 0.15mm. The copper heat-conducting plate has dimensions of 100mm×100mm×5mm and is fixed to the outer wall of the culture container with M4 bolts at a bolt spacing of 50mm. The cold end face of the thermoelectric cooler is attached to the first area of ​​the copper heat-conducting plate, and the heating resistance wire is attached to the second area, with a distance of 12mm between the two areas. The polyimide insulation film layer has a thickness of 0.1mm, covering the outside of the thermoelectric cooler and the heating resistance wire, and the edges are sealed with high-temperature resistant tape. The heat dissipation fins can be made of aluminum alloy, with a fin height of 30mm and a spacing of 5mm. The surface is covered by an axial fan with an airflow of 20CFM and an operating voltage of 24V.

[0036] The hot end of the thermoelectric cooler is connected to the heat sink assembly via thermal grease. The heat sink assembly is mounted on a bracket outside the culture vessel, 20mm from the side wall. The power supply line for the heating element is connected to the DO output terminal of the PLC controller via a relay, with a relay switching time of 10ms. The copper heat sink is positioned in the center of the side wall of the culture vessel, 150mm from both the top and bottom. The signal line of the temperature sensor is connected to the analog input interface of the PLC controller via a shielded cable, with a sampling frequency of 1Hz. When the real-time temperature is below the target range, the PLC controller outputs a high-level signal to activate the heating element; when the temperature is above the target range, the thermoelectric cooler and axial fan are activated. The proportional gain Kp of the PID control algorithm is set to 5, the integral time Ti is 60s, and the derivative time Td is 10s, ensuring a temperature regulation accuracy of ±0.3℃.

[0037] This implementation enables rapid response and precise temperature control of the culture container. The synergistic operation of the semiconductor cooling chip and heating resistance wire covers a temperature control range of 15°C to 30°C, meeting the needs of different growth stages of Aralia elata tissue culture seedlings. A copper heat-conducting plate evenly distributes heat, preventing localized overheating or overcooling. A polyimide insulation film reduces energy loss and improves temperature control efficiency. Heat dissipation fins and an axial fan ensure timely heat dissipation from the hot end of the semiconductor cooling chip, maintaining stable cooling performance. The overall structure is compact and reliable, easy to install and maintain, and suitable for factory-style seedling cultivation environments.

[0038] In another technical solution, an adjustable reflective system is provided under the supplementary lighting group, the adjustable reflective system comprising: A reflector, located 50 mm to 100 mm directly below the supplementary lighting group, is made of anodized aluminum with a reflectivity of 85% to 90%, a surface roughness Ra≤0.2 μm, and an initial tilt angle of 15° to 30°. An angle adjuster is installed on the side wall of the culture container. The angle adjuster includes a stepper motor, a planetary gearbox with a reduction ratio of 10:1, and a universal coupling. The input shaft of the planetary gearbox is connected to the output shaft of the stepper motor, and the output end of the planetary gearbox is connected to the reflector through the universal coupling. The light intensity feedback unit includes four four-quadrant photoelectric sensors, which are embedded in the four corners of the bottom of the culture container. The photosensitive surface of the four-quadrant photoelectric sensors is flush with the surface of the culture medium and 50 mm away from the side wall of the container. The drive end of the stepper motor is connected to the pulse output interface of the PLC controller via a shielded cable. The signal terminal of the photoelectric sensor is connected to the analog input interface of the PLC controller via an AD conversion module; The PLC controller performs the following steps: A1. Implement feedback adjustments, as detailed below: A11. Read the real-time light intensity values ​​of the four photoelectric sensors; A12. Calculate the uniformity of light intensity U = minimum light intensity value / maximum light intensity value; A13. When U < 0.85, determine the horizontal and vertical tilt adjustment of the reflector based on the light intensity distribution gradient between the sensors, and output a control signal to the angle adjuster to perform the angle adjustment. A2. Perform feedback adjustment every 300 seconds.

[0039] In the above technical solution, the reflector can be made of anodized aluminum, with dimensions of 400mm×300mm×1mm, a reflectivity of 88%±2%, and a surface roughness Ra controlled between 0.15μm and 0.2μm. The reflector is hinged and installed 70mm directly below the supplementary lighting assembly, with an initial tilt angle of 20°±5°. The stepper motor for the angle adjuster can be a two-phase hybrid type, with a step angle of 1.8°, an operating voltage of 24V, and an output torque of 5N·m through a planetary gearbox with a reduction ratio of 10:1. The universal coupling can be made of stainless steel, with a torsional stiffness ≥0.1N·m / °, ensuring an angle adjustment accuracy of ±0.5°. The four-quadrant photoelectric sensors can be silicon photocell type, with a photosensitive area of ​​10mm×10mm, a range of 0-10000lx, and a linear error of ±3%. They are embedded at the four corners of the bottom of the culture container, 50mm from the side wall, with the photosensitive surface flush with the surface of the culture medium.

[0040] The stepper motor's drive signal is provided by the PLC controller's pulse output interface, with a pulse frequency of 1kHz and a microstepping setting of 8 microsteps. The photoelectric sensor signal is input to the PLC controller via a 16-bit AD conversion module, with a sampling rate of 10Hz. When the light intensity uniformity U (minimum light intensity value / maximum light intensity value) is less than 0.85, the PLC calculates the horizontal (X-axis) and vertical (Y-axis) light intensity gradients based on the data from the four sensors and outputs pulse signals to drive the stepper motor. For example, if the light intensity on the left is higher than on the right, the stepper motor is controlled to tilt the reflector 2° to 5° to the right; if the light intensity on the front is lower than on the back, the reflector's forward tilt angle is increased by 3° to 6°. After each adjustment, the light intensity is re-detected after a 30-second delay until U ≥ 0.85 or the maximum number of adjustments (3 times) is reached.

[0041] This implementation dynamically optimizes the uniformity of supplemental lighting, preventing uneven growth in tissue culture seedlings due to localized light intensity differences. The high reflectivity and low-roughness surface of the reflector ensure uniform diffuse reflection of light, while the precision transmission mechanism of the angle adjuster enables fine-tuning of the tilt angle. A distributed layout of four-quadrant photoelectric sensors comprehensively monitors the light intensity distribution, and combined with the PLC's closed-loop control algorithm, the reflector matches the light output characteristics of the supplemental lighting group in real time. The system automatically performs adjustments every 300 seconds to maintain a light intensity uniformity above 0.85, ensuring consistent photosynthetic efficiency across all parts of the Aralia elata tissue culture seedlings and improving seedling uniformity.

[0042] In another technical solution, the CO2 cylinder supply system includes: The gas source unit includes a CO2 high-pressure gas cylinder and a pressure reducing and stabilizing valve. The CO2 high-pressure gas cylinder is installed on an independent gas cylinder rack outside the culture container and is connected to the inlet of the pressure reducing and stabilizing valve through a high-pressure hose. The outlet of the pressure reducing and stabilizing valve is connected to the inlet of the mass flow controller through a polyurethane gas pipe. A flow control unit includes a mass flow controller and a solenoid valve. The inlet of the mass flow controller is connected to the outlet of the pressure reducing and stabilizing valve via an air pipe, and the outlet of the mass flow controller is connected to the inlet of the solenoid valve via an air pipe. A microporous diffuser is embedded in the center of the bottom of a culture container, with the diffusion surface flush with the bottom surface of the container. The inlet of the microporous diffuser is connected to the outlet of the solenoid valve via a silicone hose. The signal input terminal of the mass flow controller is connected to the DA output module of the PLC controller; the power supply line of the solenoid valve is connected to the DO output terminal of the PLC through a relay. The PLC controller performs the following operations: B1. Read the real-time value C of the CO2 concentration sensor, in ppm; B2. If C < 400 ppm, calculate the set flow rate Q = 0.02 × (450 - C), output a current signal to the mass flow controller through the DA output module, and output a high level at the DO output terminal to open the solenoid valve; if C > 600 ppm, output a low level at the DO output terminal to close the solenoid valve and start the exhaust device. B3. Perform steps B1 to B2 above once every 60 seconds.

[0043] In the above technical solution, the CO2 high-pressure cylinder can be a 10L steel cylinder with a working pressure of 6MPa, equipped with a two-stage pressure reducing and stabilizing valve. The inlet pressure adjustment range is 0.1-6MPa, and the outlet pressure is stabilized at 0.2MPa±0.01MPa. The outlet of the pressure reducing valve is connected to a mass flow controller via a polyurethane tubing with an inner diameter of 6mm and a pressure resistance of 0.5MPa. The mass flow controller can be a thermal sensor with a range of 0-5L / min, an accuracy of ±1%FS, an output signal of 4-20mA, and a response time ≤100ms. The solenoid valve can be a normally closed two-position two-way valve with a working voltage of 24VDC, a diameter of 4mm, and a leakage rate ≤0.1mL / min. The microporous diffuser can be made of sintered stainless steel with a pore size of 10-20μm and an effective diffusion area of ​​50cm². It is connected to the outlet of the solenoid valve via a silicone hose with an inner diameter of 8mm and a length not exceeding 1m.

[0044] The set flow rate of the mass flow controller is adjusted via a 4-20mA signal output from the PLC controller's DA module, corresponding to a flow range of 0-5L / min. When the CO2 concentration sensor detects a value C below 400ppm, the PLC calculates the flow rate using the formula Q=0.02(450-C). For example, when C=350ppm, it outputs a 12mA signal (corresponding to a flow rate of 3L / min), and simultaneously, the DO terminal outputs a high level to drive the solenoid valve to open. When C>600ppm, the DO terminal outputs a low level to close the solenoid valve and activates the exhaust system. The outlet pressure of the pressure reducing and regulating valve is automatically regulated by a built-in diaphragm mechanism to ensure a constant inlet pressure for the flow controller. The microporous diffuser is installed at the center of the bottom of the culture container, with a 20mm gap between the diffusion surface and the culture medium surface, and is fixed with a bracket to prevent displacement.

[0045] This implementation method enables precise dynamic control of CO2 concentration. A pressure-reducing and stabilizing valve ensures stable gas source pressure, preventing flow fluctuations; the high-precision adjustment of the mass flow controller meets the compensation needs for different levels of gas deficiency; and the uniform gas distribution characteristics of the microporous diffuser prevent excessively high local CO2 concentrations. The system detects the concentration and adjusts the flow rate every 60 seconds to maintain the CO2 concentration within a suitable range of 400-600 ppm, avoiding photosynthetic inhibition caused by insufficient CO2 or respiratory disturbances caused by excessive concentration in tissue culture seedlings. The overall gas path structure is compact and reliable, facilitating integration with existing culture containers and meeting the high-density deployment requirements of industrialized seedling production.

[0046] In another technical solution, the temperature sensor is installed at 1 / 2 of the height of the inner wall of the culture container, 50 mm to 100 mm away from the inner wall, and is covered with an aluminum radiation shield. The light intensity sensor is installed at the center of the top of the culture container, with the photosensitive surface facing vertically downwards, at a distance of 200 mm to 300 mm from the supplemental light group; The CO2 concentration sensor is installed 300 mm to 500 mm upstream of the bottom vent of the culture container, with the air inlet facing the airflow direction and 100 mm above the bottom.

[0047] In the above technical solutions, the temperature sensor can be a PT100 platinum resistance type, with a measurement range of 0-50℃ and an accuracy of ±0.3℃. It is installed at 1 / 2 the height of the inner wall of the culture container, 80mm±5mm away from the inner wall. The sensor is covered with an aluminum radiation shield with a thickness of 1mm and lined with a 0.5mm thick polytetrafluoroethylene heat insulation layer to reduce thermal radiation interference. The light intensity sensor can be a silicon photocell type, with a range of 0-10000lx and a linear error of ±3%. It is installed at the top center of the culture container, with the photosensitive surface facing vertically downwards, and fixed by an adjustable bracket, 250mm±10mm away from the supplemental lighting group. The CO2 concentration sensor can be based on the non-dispersive infrared (NDIR) principle, with a range of 0-2000ppm and a response time ≤30s. It is installed 400mm upstream of the bottom vent of the culture container, with the air inlet tilted at 45° towards the airflow direction, 100mm±5mm from the bottom. The sensor housing is moisture-proof and integrates a temperature compensation module.

[0048] The temperature sensor's signal line uses a three-wire connection, connected to the PLC controller's RTD input module via a shielded cable. The cable length does not exceed 3m, and the shielding layer is grounded at one end. The light intensity sensor's output signal is input to the PLC via a 16-bit AD conversion module, with a sampling frequency of 5Hz and an AD conversion reference voltage of 2.5V±0.1%. The CO2 concentration sensor communicates with the PLC via an RS-485 interface, with a baud rate of 9600bps and a data frame interval of 100ms. During installation, thermally conductive silicone grease is filled between the temperature sensor's radiation shield and the container's inner wall to ensure efficient heat conduction. The light intensity sensor's bracket is equipped with a level to ensure the photosensitive surface is parallel to the supplementary lighting assembly. A deflector is installed 50mm in front of the CO2 sensor's air inlet to prevent direct airflow from interfering with the measurement.

[0049] This implementation method optimizes sensor selection and layout to ensure the accuracy and stability of environmental parameter monitoring. The radiation-proof design of the temperature sensor effectively reduces thermal radiation errors, keeping temperature measurement deviation within ±0.5℃. The centrally mounted, high-positioned light intensity sensor avoids partial obstruction and, in conjunction with the AD conversion module, achieves linear acquisition within the 0-10000 lx range. The tilted mounting and airflow guiding structure of the CO2 sensor ensure smooth airflow, with a dynamic concentration monitoring error ≤ ±30 ppm. All sensor signal transmissions employ anti-interference design, and the data acquisition cycle is synchronized to 1 second, providing real-time and reliable environmental parameter feedback for PLC control.

[0050] This invention also provides a method for the industrialized cultivation of Aralia elata tissue culture seedlings, utilizing the aforementioned industrialized cultivation system for Aralia elata tissue culture seedlings, specifically including the following steps: S1. Real-time temperature data, real-time light intensity data, and real-time CO2 concentration data are collected in the culture container through the environmental monitoring module. S2. Based on the current growth stage of the Aralia elata tissue culture seedlings, the preset light-temperature coupling parameter set is called from the PLC controller. The light-temperature coupling parameter set includes the target temperature range, the target light intensity range, and the linear relationship L = k×T + b. S3. When the real-time temperature data exceeds the target temperature range, send a command to the temperature control device to bring the temperature back to the target temperature range. S4. When the real-time light intensity data exceeds the target light intensity range, send a command to the supplementary lighting group to make the light intensity return to the target light intensity range. S5. When the real-time CO2 concentration data is below 400 ppm, send an opening command to the CO2 cylinder supply system; S6. When the real-time CO2 concentration data is higher than 600 ppm, send an activation command to the exhaust system; S7. Repeat steps S1 to S6 every 120 minutes.

[0051] In the above technical solution, the temperature sensor of the environmental monitoring module can be a PT100 platinum resistance type, installed at 1 / 2 the height of the inner wall of the culture container, 80mm±5mm from the inner wall, with a measurement range of 0-50℃ and an accuracy of ±0.3℃. The light intensity sensor can be a silicon photovoltaic cell type, installed at the center of the top of the container, with the photosensitive surface vertically downward, 250mm±10mm from the supplemental lighting group, with a range of 0-10000lx and a linear error of ±3%. The CO2 concentration sensor can be an NDIR infrared type, installed 400mm upstream of the bottom vent, with the air inlet tilted at 45° towards the airflow direction, with a range of 0-2000ppm and a response time ≤30s. The sensor signals are connected to the input module of the PLC controller via shielded cables. The temperature sensor uses a three-wire connection, while the light and CO2 sensors transmit data through a 16-bit AD conversion module and an RS-485 interface, respectively, with sampling frequencies of 1Hz, 5Hz, and 1Hz.

[0052] When the PLC controller calls the photothermal coupling parameter group, it selects preset parameters according to the growth stage of the tissue culture seedlings. For example, during the rooting stage, k=100lx / ℃, b=-1800lx, target temperature range 22-26℃, and light range 5000-7000lx are used. When the real-time temperature T is 24℃, the target light intensity L is calculated as 100×24-1800=6000lx; if the real-time light intensity is 5500lx, the PLC outputs a PWM signal to adjust the brightness of the supplementary lighting group to 6000lx±50lx. The temperature control device uses a PID algorithm for adjustment, with a proportional coefficient Kp=5 and an integral time Ti=60s, to stabilize the temperature within ±0.3℃ of the set value. In CO2 concentration control, when the detected value C=350ppm, the calculated gas supply flow rate Q=0.02×(450-350)=2L / min is used. The DA module outputs a 12mA signal to control the mass flow controller, and at the same time, the DO terminal starts the solenoid valve to supply gas.

[0053] The system executes a control process every 120 minutes. During execution, the PLC first reads all sensor data and verifies its validity, removing outliers and using the 10-second average as input. Based on the current growth stage and matching parameter set, it adjusts temperature, light intensity, and CO2 concentration sequentially. If multiple parameters exceed limits simultaneously, adjustments are made in the priority order of temperature → light intensity → CO2, with a 30-second interval between adjustments to avoid equipment conflicts. The exhaust system starts when CO2 > 600 ppm, runs for 5 minutes, and checks if the concentration has dropped below 500 ppm; otherwise, the exhaust time is extended. All operation records are stored on the PLC's SD card, including timestamps, parameter settings, and actual values, for easy traceability and analysis. This implementation method, through timing control and priority management, ensures a smooth transition of environmental parameters and avoids frequent actuator movements.

[0054] In another technical solution, the current growth stage includes a rooting stage; the light-temperature coupling parameter set of the rooting stage satisfies the following: the target temperature range is 22℃ to 26℃, the target light intensity range is 5000 lx to 7000 lx, the slope coefficient k is 100 lx / ℃, and the intercept coefficient b is -1800 lx.

[0055] In the above technical solution, the photothermal coupling parameter group for the rooting stage is set to a target temperature range of 22℃ to 26℃, a target light intensity range of 5000lx to 7000lx, and a linear relationship of L=100T-1800. When the temperature sensor detects a real-time temperature T of 24℃, the PLC controller calculates the target light intensity L=100×24-1800=6000lx. If the real-time light intensity is detected at 5500lx, the PLC adjusts the output of the supplementary lighting group via a PWM signal, gradually increasing it to 6000lx±50lx at a rate of 50lx / second. The temperature control device uses a PID algorithm for adjustment, with a proportional coefficient Kp=5 and an integral time Ti=60s. When the temperature deviates from the set value, the heating resistance wire compensates with a power of 10W / ℃ or the semiconductor cooling chip adjusts with a cooling capacity of 8W / ℃ to stabilize the temperature within the range of 24℃±0.3℃. The CO2 concentration is controlled to be maintained at 400-600ppm. When the detected value C=380ppm, the supplementary gas flow rate is adjusted according to the formula Q=0.02×(450-380)=1.4L / min.

[0056] Parameter group switching is achieved through the stage recognition function of the PLC controller. When tissue culture seedlings enter the rooting stage (days 8-15 of cultivation), the PLC automatically calls the parameter group and adjusts the control strategy simultaneously: temperature is prioritized over light adjustment; if deviations in both exist simultaneously, the temperature is first adjusted to 24℃±0.5℃, and then the light is adjusted to the calculated value after 30 seconds. The response time for CO2 concentration control is shortened to 45 seconds, and the exhaust device activation threshold is temporarily lowered to 580ppm to avoid high concentration retention. The sensor data acquisition frequency is increased to once every 20 seconds, and a moving average filter (window period of 5 minutes) is used to eliminate instantaneous fluctuations. All actuator action records are stored on an SD card, labeled "Rooting Stage Parameters," including timestamps, set values, actual values, and adjustment amounts.

[0057] This implementation optimizes environmental control to meet the physiological needs of the rooting stage. A temperature of 24℃ promotes root cell division, and a light intensity of 6000 lx matches the light saturation point at this temperature, avoiding energy waste. Dynamic CO2 compensation ensures sufficient photosynthetic substrate while preventing high concentrations from inhibiting respiration. The linear relationship of the parameter set simplifies the control logic and reduces the PLC's computational load. Priority management and timing control avoid interference caused by simultaneous operation of multiple devices, ensuring a smooth transition of environmental parameters. Actual tests show that using this parameter set can increase the rooting rate of Aralia elata tissue culture seedlings by 15%-20%, increase the average root length by 12%-18%, and significantly improve seedling uniformity.

[0058] <Example 1> Experimental content: Environmental control during the rooting stage of Aralia elata tissue culture seedlings 1. The system configuration is as follows: Culture container: Polycarbonate rectangular box (60cm×40cm×50cm, wall thickness 5mm).

[0059] Photothermal coupling parameters: During the rooting stage, L=100T−1800, the target temperature range is 22-26°C, and the light intensity is 5000-7000 lx.

[0060] CO2 control: Intake threshold 400 ppm, Exhaust threshold 600 ppm.

[0061] 2. The operating steps are as follows: S1. Environmental monitoring module collects data in real time: temperature 24.5°C, light intensity 6200 lx, CO2 concentration 380ppm.

[0062] S2 and PLC call the rooting stage parameters and calculate the target light intensity should be 100×24.5−1800=6500lx. The actual light intensity (6200 lx) is too low, so the brightness of the supplementary light group is increased to 6500 lx. S3. When the CO2 concentration is below 400 ppm, start the CO2 cylinder supply system and set the flow rate to 0.02×(450−380)=1.4L / min.

[0063] S4. Continuous monitoring: Stop gas supply when CO2 concentration rises to 450 ppm. Within ±0.3°C temperature fluctuation, light intensity uniformity U=0.88 (>0.85), no need to adjust reflector.

[0064] 3. Experimental results: The rooting rate of tissue culture seedlings increased to 95%, and the root length was 20% longer than that of the control group.

[0065] <Comparative Example 1> Traditional threshold control (without photothermal coupling) 1. The system configuration is as follows: independent temperature control (22-26°C), fixed illumination of 6000 lx, and CO2 threshold control is the same as in Example 1.

[0066] 2. The operating steps are as follows: At a temperature of 25°C, the light intensity was fixed at 6000 lx (non-dynamically adjustable).

[0067] When the CO2 concentration is below 400 ppm, gas is added, but the gas addition rate is fixed at 1 L / min (non-dynamic calculation).

[0068] 3. Experimental Results Compared to the system of this invention: Poor light-temperature synergy reduced the photosynthetic efficiency of tissue culture seedlings by 10%.

[0069] Large fluctuations in CO2 concentration (350-650 ppm) resulted in uneven growth, with a rooting rate of only 80%.

[0070] Although the embodiments of the present invention have been disclosed above, they are not limited to the applications listed in the specification and embodiments. They can be applied to various fields suitable for the present invention. For those skilled in the art, other modifications can be easily made. Therefore, without departing from the general concept defined by the claims and their equivalents, the present invention is not limited to the specific details.

Claims

1. A factory-scale seedling production system for Aralia elata tissue culture seedlings, characterized in that, include: The culture container is a rectangular box. The environmental monitoring module includes a temperature sensor, a light intensity sensor, and a CO2 concentration sensor installed inside the culture container; The PLC controller has pre-stored a set of photothermal coupling parameters for different growth stages. The set of photothermal coupling parameters defines a linear relationship between the target temperature range and the target light intensity range: L = k×T + b, where L is the light intensity in lx, T is the temperature in °C, k is the slope coefficient ranging from 80 lx / °C to 120 lx / °C, and b is the intercept coefficient ranging from -2000 lx to -1600 lx. The execution module includes a temperature control device, a supplementary lighting unit, a CO2 gas cylinder supply system, and an exhaust device. The PLC controller is connected to the environmental monitoring module via a data acquisition unit and to the execution module via the Modbus-RTU communication protocol; the PLC controller performs the following operations: S1 receives real-time data from temperature sensor, light intensity sensor and CO2 concentration sensor; S2. Call the corresponding light and temperature coupling parameter group according to the current growth stage of the Aralia elata tissue culture seedling; S3. When the real-time temperature deviates from the target temperature range, an adjustment command is sent to the temperature control device; When the real-time light intensity deviates from the target light intensity range, an adjustment command is sent to the supplementary lighting group; when the real-time CO2 concentration is below 400ppm, an opening command is sent to the CO2 cylinder supply system; when the real-time CO2 concentration is above 600ppm, an opening command is sent to the exhaust device.

2. The Aralia elata tissue culture seedling industrialization system as described in claim 1, characterized in that, The top of the culture container is provided with a first sliding groove along its length direction, a first sliding block is provided in the first sliding groove, a mounting base is fixedly connected to the first sliding block, and a second sliding groove is provided on the mounting base along the width direction of the culture container, a second sliding block is provided in the second sliding groove, and a mounting block is fixedly connected to the second sliding block. The supplementary lighting assembly includes an adjustable height bracket and a light source module. The fixed end of the bracket is fixedly connected to the mounting block, and the adjustable end of the bracket is connected to the light source module. The light source module includes a substrate, an LED array soldered on the substrate, and a light mixing lens. The LED array includes multiple red LEDs and multiple blue LEDs. The peak wavelength of the red LEDs is 660 nm ± 5 nm, and the peak wavelength of the blue LEDs is 450 nm ± 5 nm. The red LEDs and blue LEDs are arranged in a rectangular grid to form an LED array, with three red LEDs and one blue LED forming a basic unit. The spacing between the rectangular grids is 15~20 mm. The light mixing lens is connected to the substrate by a snap-fit. The light mixing lens is located below the LED array and at a distance of 5-8 mm from the LED array. The material of the light mixing lens is PMMA.

3. The Aralia elata tissue culture seedling industrialization system as described in claim 1, characterized in that, The temperature control device includes a semiconductor cooling chip and a heating resistance wire; arranged sequentially from the inside to the outside on the outer surface of the culture container: A thermally conductive silicone grease layer, with a thickness of 0.1~0.2 mm, is directly coated onto the outer surface of the culture container. A copper heat-conducting plate is bolted to the outer wall of the culture container; The cold end face of the semiconductor cooling chip is bonded to the first area of ​​the outer surface of the copper heat-conducting plate by thermal grease. The heating resistance wire is wound at equal intervals on the alumina ceramic skeleton with a wire spacing of 1-2 mm. The heating resistance wire is attached to the second area of ​​the outer surface of the copper heat-conducting plate through thermal grease. The distance between the first area and the second area is 10-15 mm. A polyimide heat insulation film layer is used to cover the semiconductor and the heating resistance wire; The hot end of the semiconductor cooling chip extends to the outside of the polyimide insulation film and is connected to a heat dissipation fin assembly, the surface of which is covered with an axial fan.

4. The Aralia elata tissue culture seedling industrialization system as described in claim 3, characterized in that, The supplementary lighting assembly is equipped with an adjustable reflective system, which includes: A reflector, located 50 mm to 100 mm directly below the supplementary lighting group, is made of anodized aluminum with a reflectivity of 85% to 90%, a surface roughness Ra≤0.2 μm, and an initial tilt angle of 15° to 30°. An angle adjuster is installed on the side wall of the culture container. The angle adjuster includes a stepper motor, a planetary gearbox with a reduction ratio of 10:1, and a universal coupling. The input shaft of the planetary gearbox is connected to the output shaft of the stepper motor, and the output end of the planetary gearbox is connected to the reflector through the universal coupling. The light intensity feedback unit includes four four-quadrant photoelectric sensors, which are embedded in the four corners of the bottom of the culture container. The photosensitive surface of the four-quadrant photoelectric sensors is flush with the surface of the culture medium and 50 mm away from the side wall of the container. The drive end of the stepper motor is connected to the pulse output interface of the PLC controller via a shielded cable. The signal terminal of the photoelectric sensor is connected to the analog input interface of the PLC controller via an AD conversion module; The PLC controller performs the following steps: A1. Implement feedback adjustments, as detailed below: A11. Read the real-time light intensity values ​​of the four photoelectric sensors; A12. Calculate the uniformity of light intensity U = minimum light intensity value / maximum light intensity value; A13. When U < 0.85, determine the horizontal and vertical tilt adjustment of the reflector based on the light intensity distribution gradient between the sensors, and output a control signal to the angle adjuster to perform angle adjustment. A2. Perform feedback adjustment every 300 seconds.

5. The Aralia elata tissue culture seedling industrialization system as described in claim 3, characterized in that, The CO2 cylinder supply system includes: The gas source unit includes a CO2 high-pressure gas cylinder and a pressure reducing and stabilizing valve. The CO2 high-pressure gas cylinder is installed on an independent gas cylinder rack outside the culture container and is connected to the inlet of the pressure reducing and stabilizing valve through a high-pressure hose. The outlet of the pressure reducing and stabilizing valve is connected to the inlet of the mass flow controller through a polyurethane gas pipe. A flow control unit includes a mass flow controller and a solenoid valve. The inlet of the mass flow controller is connected to the outlet of the pressure reducing and stabilizing valve via an air pipe, and the outlet of the mass flow controller is connected to the inlet of the solenoid valve via an air pipe. A microporous diffuser is embedded in the center of the bottom of a culture container, with the diffusion surface flush with the bottom surface of the container. The inlet of the microporous diffuser is connected to the outlet of the solenoid valve via a silicone hose. The signal input terminal of the mass flow controller is connected to the DA output module of the PLC controller; the power supply line of the solenoid valve is connected to the DO output terminal of the PLC through a relay. The PLC controller performs the following operations: B1. Read the real-time value C of the CO2 concentration sensor, in ppm; B2. If C < 400 ppm, calculate the set flow rate Q = 0.02 × (450 - C), output a current signal to the mass flow controller through the DA output module, and output a high level at the DO output terminal to open the solenoid valve; if C > 600 ppm, output a low level at the DO output terminal to close the solenoid valve and start the exhaust device. B3. Perform steps B1 to B2 above once every 60 seconds.

6. The Aralia elata tissue culture seedling industrialization system as described in claim 1, characterized in that, The temperature sensor is installed at 1 / 2 the height of the inner wall of the culture container, 50 mm to 100 mm away from the inner wall, and is covered with an aluminum radiation shield. The light intensity sensor is installed at the center of the top of the culture container, with the photosensitive surface facing vertically downwards, at a distance of 200 mm to 300 mm from the supplemental lighting group; The CO2 concentration sensor is installed 300 mm to 500 mm upstream of the bottom vent of the culture container, with the air inlet facing the airflow direction and 100 mm above the bottom.

7. The method for industrialized seedling production of Aralia elata tissue culture seedlings as described in claim 1, characterized in that, The process, using the Aralia elata tissue culture seedling industrialization seedling production system as described in any one of claims 1 to 6, specifically includes the following steps: S1. Real-time temperature data, real-time light intensity data, and real-time CO2 concentration data are collected in the culture container through the environmental monitoring module. S2. Based on the current growth stage of the Aralia elata tissue culture seedlings, the preset light-temperature coupling parameter set is called from the PLC controller. The light-temperature coupling parameter set includes the target temperature range, the target light intensity range, and the linear relationship L = k×T + b. S3. When the real-time temperature data exceeds the target temperature range, send a command to the temperature control device to bring the temperature back to the target temperature range. S4. When the real-time light intensity data exceeds the target light intensity range, send a command to the supplementary lighting group to make the light intensity return to the target light intensity range. S5. When the real-time CO2 concentration data is below 400 ppm, send an opening command to the CO2 cylinder supply system; S6. When the real-time CO2 concentration data is higher than 600 ppm, send an activation command to the exhaust system; S7. Repeat steps S1 to S6 every 120 minutes.

8. The method for industrialized seedling production of Aralia elata tissue culture seedlings as described in claim 1, characterized in that, The current growth stage includes the rooting stage; the light-temperature coupling parameter set of the rooting stage satisfies the following: target temperature range of 22℃ to 26℃, target light intensity range of 5000 lx to 7000 lx, slope coefficient k of 100 lx / ℃, and intercept coefficient b of -1800 lx.