Layered stem cell incubator

Abstract

The invention relates to the technical field of stem cell culture, in particular to a layered stem cell culture box which comprises a culture box body, a limiting mechanism and an anti-falling mechanism, the culture box body is provided with a containing cavity, a box door is hinged to an opening of the containing cavity, the limiting mechanism is used for limiting a round vessel, and the anti-falling mechanism is used for preventing the round vessel from falling off. The damage caused by the sliding or falling of the vessel when the articles in the incubator are carried, stored and taken can be effectively avoided by limiting the vessel; the anti-falling mechanism is used for preventing the layered placing plate from falling off when being pulled and preventing the layered placing plate from being separated from the placing cavity of the incubator body when the layered placing plate is pulled too fast, and when an experimenter pulls the layered placing plate, the connecting shaft slides along the anti-falling groove and is automatically embedded into the corrugated groove of the anti-falling groove when the experimenter stops pulling the layered placing plate; the layering plate is prevented from being completely separated from the placing cavity due to overexertion or misoperation, and the culture vessel is prevented from falling and being damaged.

Description

Technical Field

[0001] The present invention relates to the technical field of stem cell culture, in particular to a layered stem cell culture box. Background Art

[0002] A stem cell incubator is a specialized device used to maintain the survival, proliferation, and differentiation of stem cells. By precisely controlling environmental parameters such as temperature, humidity, and gas concentration, it simulates the in vivo physiological environment and ensures the stability of the biological properties of stem cells. It is a core device in fields such as basic stem cell research, clinical treatment, and drug development. After powering on the device, the temperature and CO2 concentration are set, and then a culture vessel lined with Matrigel is placed in the incubator for pre-incubation. To revive cells, cryopreserved tubes are quickly thawed, centrifuged, resuspended in complete culture medium, and inoculated into the pre-treated culture vessel.

[0003] Generally, stem cell culture vessels are plastic round vessels that are suitable for cell growth and reproduction, and are especially convenient for observing and selecting single-cell colonies. They have large openings and are easy to operate. The culture vessels are placed directly on the placement rack and then pushed into the incubator for culture. Since the contact area between the circular vessel and the placement rack is small, if the force is uneven or the operation is too fast when pushing it into the incubator, it is easy to cause the vessel to tilt, slide, or even overflow the culture fluid, which may cause contamination or cell damage. In addition, the placement plate and the incubator are limited by protruding limit strips. When pulling the placement plate, excessive force will cause the placement plate to quickly detach from the placement cavity of the incubator. The culture vessel will shake violently due to inertia, which may cause the culture fluid to overflow outside the vessel or even splash into the inside of the incubator. The overflowed culture fluid may not only contaminate other samples, but also cause cell number loss or local osmotic pressure changes, affecting the activity of stem cells. Summary of the Invention

[0004] The object of the present invention is to provide a layered stem cell culture chamber to solve the problems raised by the above background technology.

[0005] The technical solution adopted by the present application to solve the technical problem is: a layered stem cell culture box, comprising: a culture box body, the culture box body is provided with a placement cavity, the placement box opening is hingedly provided with a box door, and further comprising: A limiting mechanism is provided in the placement cavity, the limiting mechanism comprising layered placement plates equidistantly provided in the placement cavity, a first limiting disc and a second limiting disc provided on the layered placement plates, and the limiting mechanism is used to limit the position of the culture vessel; An anti-falling mechanism is arranged on both sides of the limiting mechanism. The anti-falling mechanism includes fixing strips arranged at the bottom ends of both sides of the layered placement plate. A card slot is opened on the top of the fixing strip. The bottom of the layered placement plate slides in the card slot. The anti-falling mechanism is used to prevent the layered placement plate from falling off when it is pulled.

[0006] Preferably, a ventilation hole is provided on one side of the incubator body, a support is fixedly provided on the bottom of the incubator body, a sealing strip is fixedly provided on one side of the opening of the placement cavity, a handle is fixedly provided on one side of the door, and a control panel is provided on the side of the door close to the handle.

[0007] Preferably, the limiting mechanism also includes a straight slot extending through the layered placement plate, a fixed shaft is fixedly provided on the top of the layered placement plate, a rotating disk is rotatably provided on the outer surface of the fixed shaft, and resistance is provided between the rotating disk and the fixed shaft.

[0008] Preferably, a fixed disk is fixedly provided on the top of the rotating disk, and a limiting groove is provided at the bottom of the fixed disk. A section of the limiting groove is recessed toward the center of the fixed disk, and a roller is slidably provided in the limiting groove, and a limiting groove extends from the bottom of the roller.

[0009] Preferably, a plurality of guide rails are fixedly and symmetrically arranged on the rotating disk, and the plurality of guide rails are arranged in a circular array. The guide rails are fixedly connected to the first limiting disk, and are slidably connected to the second limiting disk.

[0010] Preferably, arc grooves are provided on both the limit plate 1 and the limit plate 2, and the arc grooves on the limit plate 1 and the limit plate 2 are arranged relative to each other. A connecting rod is fixedly provided on one side of the limit plate 2, and the end of the connecting rod away from the limit plate 2 is fixedly connected to the roller shaft.

[0011] Preferably, the anti-falling mechanism further comprises a slide groove provided on the fixing bar, the slide groove and the card slot are communicated with each other, a sliding bar is provided in the slide groove, and the top of the sliding bar is fixedly connected to the bottom of the layered placement plate.

[0012] Preferably, connecting bars are symmetrically fixedly provided on the sliding bar, the sliding bar slides in the sliding groove through the connecting bars, and anti-slip grooves are symmetrically opened through the fixing bar.

[0013] Preferably, a connecting shaft is symmetrically fixed on the sliding bar, a connecting piece is rotatably provided on the connecting shaft, a moving shaft is fixedly provided on one end of the connecting piece away from the connecting shaft, and the moving shaft is slidably provided in the anti-slip groove.

[0014] Preferably, a groove is provided on the connecting piece, a notch is provided on one side of the groove, an elastic piece is movably provided in the groove, and fixed blocks are fixedly provided on both sides of one end of the elastic piece away from the groove, and the fixed blocks are fixedly connected to the side walls of the connecting piece.

[0015] The beneficial effects of this application are: This application provides a tiered stem cell incubator that positions culture dishes between the arc-shaped grooves of a first and second limiting plate. This mechanism allows researchers to quickly and accurately position the culture dishes in designated locations without repeated adjustments, improving operational efficiency. This secure positioning of the dishes effectively prevents damage caused by slipping or falling during transport, storage, and access within the incubator, protecting valuable stem cell samples and experimental data.

[0016] The present application provides a layered stem cell culture incubator. When the layered placement plate is pulled through a straight notch, the sliding bar pulls the movable shaft to move within the anti-slip groove. The connecting piece is pressed against the notch by an elastic piece, causing the connecting shaft to be stuck in the continuous corrugated section of the anti-slip groove. When the pull stops, the position of the sliding bar can be limited by the connecting shaft being stuck in the anti-slip groove. Its position remains relatively fixed, preventing the layered placement plate from being pulled too quickly and being separated from the placement cavity of the incubator body. The engagement of the continuous corrugated section and the connecting shaft forms a physical locking effect. When the experimenter pulls the layered placement plate, the connecting shaft slides along the anti-slip groove and automatically embeds into the corrugated groove of the anti-slip groove when it stops, preventing the layered plate from completely falling out of the placement cavity due to excessive force or misoperation, and preventing the culture vessel from falling and being damaged.

[0017] The present application provides a layered stem cell culture box.

[0018] In addition to the above-described purposes, features and advantages, the present application has other purposes, features and advantages. The present application will be further described in detail below with reference to the drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 This is a schematic diagram of the internal structure of the placement chamber of the present invention; Figure 3 This is a schematic diagram of the cross-section structure of the incubator body of the present invention; Figure 4 It is a schematic diagram of the cross-section structure of the fixed disk of the present invention; Figure 5 This is a schematic diagram of the explosion structure of the limiting mechanism of the present invention; Figure 6 This is a schematic diagram of the cross-sectional structure of the incubator side wall of the present invention; Figure 7 For the present invention Figure 6 Schematic diagram of the enlarged structure at A in the middle; Figure 8 It is a schematic structural diagram of the anti-falling mechanism of the present invention.

[0020] Description of the figure number: 1. Incubator body; 2. Placement cavity; 3. Chamber door; 4. Ventilation port; 5. Support; 6. Sealing strip; 7. Handle; 8. Control panel; 9. Limiting mechanism; 10. Layered placement plate; 11. Straight slot; 12. Fixed shaft; 13. Fixed plate; 14. Limiting slot; 15. Roller; 16. Rotating plate; 17. Guide rail; 18. Limiting plate 1; 19. Limiting plate 2; 20. Arc groove; 21. Connecting rod; 22. Anti-falling mechanism; 23. Fixed strip; 24. Slot; 25. Slide; 26. Sliding strip; 27. Connecting strip; 28. Anti-falling slot; 29. ​​Connecting shaft; 30. Connecting piece; 31. Moving shaft; 32. Groove; 33. Notch; 34. Elastic piece; 35. Fixed block. DETAILED DESCRIPTION

[0021] It should be noted that, in the absence of conflict, the embodiments and features of the embodiments in this application can be combined with each other. The present application will be described in detail below with reference to the accompanying drawings and in combination with the embodiments.

[0022] In order to enable those skilled in the art to better understand the present invention, the following will provide a clear and complete description of the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work should fall within the scope of protection of the present invention.

[0023] Please refer to Figures 1 to 8 A layered stem cell culture box includes: a culture box body 1, a placement cavity 2 provided in the culture box body 1, a door 3 hingedly provided at the opening of the placement box, a ventilation port 4 provided on one side of the culture box body 1, and a detachable filter installed at the ventilation port 4, which can effectively filter impurities and microorganisms in the outside air to avoid contamination of the culture environment; a support 5 fixedly provided at the bottom of the culture box body 1, made of non-slip rubber material, which not only enhances the stability of the equipment but also effectively reduces the transmission of vibration during operation; a sealing strip 6 fixedly provided on one side of the opening of the placement cavity 2, which can form a tight sealing structure when the door 3 is closed, preventing the intrusion of external air and the leakage of the internal culture environment. A handle 7 is fixedly provided on one side of the door 3, and a control panel 8 is provided on the side of the door 3 close to the handle 7.

[0024] It should be noted that the incubator body 1 also houses a temperature control system, including a temperature sensor, heating elements, and cooling elements. The temperature sensor monitors the temperature within the incubation chamber 2 in real time and feeds this data back to the control panel 8, which automatically adjusts the operating state of the heating and cooling elements according to preset temperature values ​​to maintain a stable incubation temperature.

[0025] Please refer to the Figures 1 to 6 A layered stem cell culture chamber further includes a limiting mechanism 9, which is disposed within the placement chamber 2. The limiting mechanism 9 includes layered placement plates 10 equidistantly disposed within the placement chamber 2. The layered placement plates 10 are provided with a first limiting disc 18 and a second limiting disc 19. The limiting mechanism 9 is used to limit the position of the culture vessels. Stem cell culture requires extremely high environmental stability. The limiting mechanism 9 can prevent the culture vessels from being displaced due to factors such as vibration during incubator operation and inertia during door opening, thereby preventing the culture medium from shaking or even spilling, and maintaining the stable microenvironment required for cell growth. Limiting can reduce the risk of contact between the vessels and the outside world, such as preventing the chamber door 3 from colliding with other components when closed due to vessel shaking, thereby reducing the possibility of external contamination intrusion and ensuring the cleanliness of the culture environment.

[0026] The limiting mechanism 9 further includes a straight slot 11 extending through the layered placement plate 10, through which the operator can pull the layered placement plate 10 out of the placement chamber 2. A fixed shaft 12 is fixedly provided on the top of the layered placement plate 10, and a rotating disk 16 is rotatably provided on the outer surface of the fixed shaft 12. A resistance is provided between the rotating disk 16 and the fixed shaft 12. A fixed disk 13 is fixedly provided on the top of the rotating disk 16, and a limiting slot 14 is provided at the bottom of the fixed disk 13. A section of the limiting slot 14 is recessed toward the center of the fixed disk 13. A roller 15 is slidably provided within the limiting slot 14, and the bottom of the roller 15 extends out of the limiting slot 14.

[0027] It is worth mentioning that multiple sets of guide rails 17 are symmetrically fixedly mounted on the rotating disk 16. The multiple sets of guide rails 17 are arranged in a circular array. The guide rails 17 are fixedly connected to the first limit plate 18, and are slidably connected to the second limit plate 19. Both the first limit plate 18 and the second limit plate 19 are provided with arcuate slots 20 extending therethrough. The arcuate slots 20 on the first limit plate 18 and the second limit plate 19 are arranged opposite each other. A connecting rod 21 is fixedly mounted on one side of the second limit plate 19. The end of the connecting rod 21 away from the second limit plate 19 is fixedly connected to the roller shaft 15.

[0028] By rotating the rotating disk 16 along the fixed axis 12, when the rotating disk 16 rotates, a relative displacement is generated between the rotating disk 16 and the fixed disk 13. When the rotating disk 16 rotates, the limiting groove 14 in the fixed disk 13 drives one of the rollers 15 to move toward the center of the fixed disk 13. During this process, the roller 15 pulls the limiting disk 2 19 through the connecting rod 21, causing the limiting disk 2 19 to move away from the limiting disk 2 19. At this time, the culture dish can be placed between the limiting disk 18 and the arc groove 20 of the limiting disk 2 19, and the culture dish is close to the arc groove 20 of the limiting disk 18. When the rotating disk 16 rotates again, under the restriction of the limiting groove 14, the roller 15 pushes the limiting disk 2 19 gradually closer to and fits the limiting disk 18 through the connecting rod 21, thereby limiting the culture dish. The experimenter can quickly and accurately place the culture vessel at the specified position through the limiting mechanism 9 without repeated adjustment, thereby improving operating efficiency.

[0029] Please refer to the Figure 2 、 Figure 4 as well as Figures 6 to 8 A tiered stem cell culture chamber further includes an anti-drop mechanism 22 disposed on either side of the limiting mechanism 9. The anti-drop mechanism 22 includes fixing bars 23 disposed at the bottom ends of the tiered placement plates 10. The fixing bars 23 have slots 24 defined at their tops, into which the bottoms of the tiered placement plates 10 slide. The anti-drop mechanism 22 is used to prevent the tiered placement plates 10 from falling when pulled out. The anti-drop mechanism 22 also includes a chute 25 defined on the fixing bars 23. The chute 25 communicates with the slots 24. A sliding bar 26 is disposed within the chute 25, the top of which is fixedly connected to the bottom of the tiered placement plates 10.

[0030] Connecting bars 27 are symmetrically fixed to the sliding bar 26, allowing the sliding bar 26 to slide within the chute 25 via the connecting bars 27. Anti-slip grooves 28 are symmetrically formed through the fixed bar 23. Connecting shafts 29 are symmetrically fixed to the sliding bar 26, on which a connecting piece 30 is rotatably mounted. A movable shaft 31 is fixedly mounted on the end of the connecting piece 30 away from the connecting shaft 29, and the movable shaft 31 slides within the anti-slip groove 28. Each connecting piece 30 has a groove 32, and a notch 33 is formed on one side of each connecting piece 30. Each groove 32 has a resilient piece 34 movably mounted therein. Fixing blocks 35 are fixedly mounted on both sides of the end of the resilient piece 34 away from the groove 32, and the fixing blocks 35 are fixedly connected to the sidewalls of the connecting piece 30.

[0031] When the layered placement plate 10 needs to be pulled out to place a culture dish, the experimenter can pull the layered placement plate 10 through the straight notch 11. At this time, the sliding bar 26 will pull the movable shaft 31 to move in the anti-slip groove 28. The connecting piece 30 is pressed against the notch 33 by the elastic piece 34, prompting the connecting shaft 29 to be stuck in the continuous corrugated section of the anti-slip groove 28. When the pulling stops, the position of the sliding bar 26 can be limited by the connecting shaft 29 in the anti-slip groove 28, and its position remains relatively fixed, preventing the layered placement plate 10 from being separated from the placement cavity 2 of the incubator body 1 when it is pulled too fast. The engagement of the continuous corrugated section and the connecting shaft 29 forms a physical locking effect. When the experimenter pulls the layered placement plate 10, the connecting shaft 29 slides along the anti-slip groove 28 and automatically embeds into the corrugated groove of the anti-slip groove 28 when it stops, preventing the layered plate from being completely separated from the placement cavity 2 due to excessive force or misoperation, thereby preventing the culture vessel from falling and being damaged.

[0032] The working principle of the present invention is specifically as follows: When the experimenter opens the box door 3 and pulls the layered placement plate 10 through the straight notch 11, the sliding bar 26 will pull the movable shaft 31 to move in the anti-slip groove 28, and the connecting piece 30 will be pressed against the notch 33 by the elastic piece 34, prompting the connecting shaft 29 to be stuck in the continuous corrugated section of the anti-slip groove 28. When the pulling stops, the position of the sliding bar 26 can be limited by the connecting shaft 29 stuck in the anti-slip groove 28, and its position remains relatively fixed, preventing the layered placement plate 10 from being separated from the placement cavity 2 of the incubator body 1 when it is pulled too fast. When the experimenter pulls the layered placement plate 10, the connecting shaft 29 slides along the anti-slip groove 28 and automatically embeds into the corrugated groove of the anti-slip groove 28 when it stops, avoiding the layered placement plate from being completely separated from the placement cavity 2 due to excessive force or misoperation, thereby preventing the culture vessel from falling and being damaged. Then, by rotating the rotating disk 16 along the fixed axis 12, when When the rotating disk 16 rotates, a relative displacement occurs between it and the fixed disk 13. When the rotating disk 16 rotates, the limiting groove 14 in the fixed disk 13 drives one of the rollers 15 to move toward the center of the fixed disk 13. During this process, the roller 15 pulls the limiting disk 2 19 through the connecting rod 21, causing the limiting disk 2 19 to move away from the limiting disk 2 19. At this time, the culture dish can be placed between the limiting disk 18 and the arc groove 20 of the limiting disk 2 19, and the culture dish is close to the arc groove 20 of the limiting disk 18. When the rotating disk 16 rotates again, under the restriction of the limiting groove 14, the roller 15 pushes the limiting disk 2 19 gradually closer to and fits the limiting disk 18 through the connecting rod 21, thereby limiting the circular culture dish. The experimenter can quickly and accurately place the culture vessel at the specified position through the limiting mechanism 9 without repeated adjustments, thereby improving operating efficiency. The firm positioning of the vessels can effectively prevent damage caused by sliding or falling of the vessels when transporting, storing or retrieving items in the incubator, thus protecting precious stem cell samples and experimental data.

[0033] It should be understood by those skilled in the art that the discussion of any of the above embodiments is merely illustrative, and within the scope of the present invention, the technical features of the above embodiments or different embodiments may be combined, the steps may be implemented in any order, and there are many other variations of the different aspects of the present invention as described above, which are not provided in detail for the sake of simplicity.

[0034] The present invention is intended to cover all such substitutions, modifications and variations that fall within the broad scope of the appended claims. Therefore, any omissions, modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A layered stem cell culture chamber, comprising: An incubator body (1) is provided with a placement cavity (2), a door (3) is hingedly provided at the opening of the placement box, and is characterized in that it further comprises: A limiting mechanism (9), wherein the limiting mechanism (9) is arranged in the placement cavity (2), and the limiting mechanism (9) comprises layered placement plates (10) equidistantly arranged in the placement cavity (2), and a limiting disk 1 (18) and a limiting disk 2 (19) are provided on the layered placement plates (10), and the limiting mechanism (9) is used to limit the culture vessel; An anti-falling mechanism (22) is provided on both sides of the limiting mechanism (9), and the anti-falling mechanism (22) comprises fixing bars (23) provided at the bottom ends of both sides of the layered placement plate (10), a card slot (24) is provided at the top of the fixing bar (23), and the bottom of the layered placement plate (10) slides in the card slot (24). The anti-falling mechanism (22) is used to prevent the layered placement plate (10) from falling off when being pulled.

2. The layered stem cell culture chamber according to claim 1, characterized in that: A ventilation opening (4) is provided on one side of the incubator body (1), a support (5) is fixedly provided on the bottom of the incubator body (1), a sealing strip (6) is fixedly provided on one side of the opening of the placement cavity (2), a handle (7) is fixedly provided on one side of the door (3), and a control panel (8) is provided on the side of the door (3) close to the handle (7).

3. The layered stem cell culture chamber according to claim 1, characterized in that: The limiting mechanism (9) further comprises a straight slot (11) extending through the layered placement plate (10); a fixed shaft (12) is fixedly provided on the top of the layered placement plate (10); a rotating disk (16) is rotatably provided on the outer surface of the fixed shaft (12); and a resistance is provided between the rotating disk (16) and the fixed shaft (12).

4. The layered stem cell culture chamber according to claim 3, characterized in that: A fixed disk (13) is fixedly arranged on the top of the rotating disk (16), and a limiting groove (14) is provided at the bottom of the fixed disk (13). A section of the limiting groove (14) is recessed toward the center of the fixed disk (13). A roller (15) is slidably arranged in the limiting groove (14), and the limiting groove (14) extends from the bottom of the roller (15).

5. The layered stem cell culture chamber according to claim 4, characterized in that: A plurality of guide rails (17) are fixedly and symmetrically arranged on the rotating disk (16), and the plurality of guide rails (17) are arranged in a circular array. The guide rails (17) are fixedly connected to the first limiting disk (18), and the guide rails (17) are slidably connected to the second limiting disk (19).

6. The layered stem cell culture chamber according to claim 5, characterized in that: The limiting plate 1 (18) and the limiting plate 2 (19) are both provided with an arc groove (20), and the arc grooves (20) on the limiting plate 1 (18) and the limiting plate 2 (19) are arranged opposite to each other. A connecting rod (21) is fixedly provided on one side of the limiting plate 2 (19), and the end of the connecting rod (21) away from the limiting plate 2 (19) is fixedly connected to the roller shaft (15).

7. The layered stem cell culture chamber according to claim 1, characterized in that: The anti-falling mechanism (22) further comprises a sliding groove (25) provided on the fixing bar (23), wherein the sliding groove (25) and the clamping groove (24) are in communication with each other, and a sliding bar (26) is provided in the sliding groove (25), wherein the top of the sliding bar (26) is fixedly connected to the bottom of the layered placement plate (10).

8. The layered stem cell culture chamber according to claim 7, characterized in that: A connecting strip (27) is symmetrically fixed on the sliding strip (26), and the sliding strip (26) slides in the sliding groove (25) through the connecting strip (27). An anti-slip groove (28) is symmetrically opened through the fixing strip (23).

9. The layered stem cell culture chamber according to claim 8, characterized in that: A connecting shaft (29) is symmetrically fixedly provided on the sliding bar (26), a connecting piece (30) is rotatably provided on the connecting shaft (29), a moving shaft (31) is fixedly provided at one end of the connecting piece (30) away from the connecting shaft (29), and the moving shaft (31) is slidably provided in the anti-slip groove (28).

10. The layered stem cell culture chamber according to claim 9, characterized in that: The connecting pieces (30) are each provided with a groove (32), a notch (33) is provided on one side of the groove (32) and an elastic piece (34) is movably provided in the groove (32), and fixed blocks (35) are fixedly provided on both sides of one end of the elastic piece (34) away from the groove (32), and the fixed blocks (35) are fixedly connected to the side wall of the connecting piece (30).

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