A stem cell culture device

Abstract

The application discloses a kind of stem cell culture devices, belong to stem cell technical field, including sealed incubator, syringe, the inside of the sealed incubator is equipped with electric rotating table, the top of the electric rotating table is equipped with adjustable shaking assembly, the adjustable shaking assembly is equipped with petri dish carrier, petri dish limiting component, the petri dish limiting component is equipped with uniform feeding assembly, the top of the sealed incubator is equipped with liquid injection pipe, the top of the liquid injection pipe is equipped with sealed iris component, the outside of the sealed incubator is equipped with lifting assembly.The adjustable shaking assembly, petri dish limiting component, uniform feeding assembly of being equipped with, so that petri dish realizes multidimensional periodicity shaking, promotes stem cell to adhere to the growth and material exchange;Sealed iris component, syringe are set, in the process of injecting reagent, the sealing of incubator is always maintained, avoid exposing sample in air, guarantee the stability of culture environment.

Description

Technical Field

[0001] This invention belongs to the field of stem cell technology, specifically relating to a stem cell culture device. Background Technology

[0002] With the continuous development of science and technology, cell engineering has been widely applied, achieving remarkable results in both the medical and pharmaceutical fields. Cell culture, as the foundation of the entire cell engineering process, plays a crucial role.

[0003] Stem cells are a type of pluripotent cell with the ability to self-renew. Under certain conditions, they can differentiate into various functional cells. Based on their developmental stage, stem cells are classified into embryonic stem cells and adult stem cells. Stem cells are undifferentiated, immature cells with the potential to regenerate various tissues, organs, and the human body; they are known in the medical field as "universal cells." Therefore, stem cell culture is extremely important.

[0004] Currently, most existing stem cell culture devices are static cultures, which prevents stem cells in the culture dish from being evenly exposed to nutrients and oxygen. This results in slow growth and proliferation of stem cells, reducing the efficiency of stem cell culture. Furthermore, in the process of single-cell culture, different key components (such as amino acids, vitamins, and inorganic salts) need to be injected into each culture sample to maintain its growth, proliferation, and pluripotency. However, the incubator needs to be turned on and off frequently during operation. This can easily cause drastic changes in internal temperature, expose the sample to a non-ideal environment, and even create the risk of cross-contamination, disrupting the stability of the overall culture environment and thus affecting the growth status of the sample. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to overcome the shortcomings of the prior art and provide a stem cell culture device.

[0006] The technical solution adopted to solve the above technical problems is: a stem cell culture device, including a sealed culture box and a syringe, wherein the side wall of the injection port of the syringe is provided with a connecting thread, an electric rotating platform is fixedly installed at the bottom of the sealed culture box, and an adjustable shaking component is provided above the electric rotating platform to dynamically improve the efficiency of stem cell culture.

[0007] The adjustable shaking assembly is equipped with a culture dish support frame for positioning and supporting multiple culture dishes, and a culture dish limiting assembly for pressing and limiting the culture dishes. The upper surface of the culture dish support frame is fixed with several placement slots in a circular array. The culture dish limiting assembly is equipped with several auxiliary feeding and mixing uniform feeding components. The uniform feeding components are all positioned corresponding to the placement slots. The top of the sealed incubator is slidably connected to several injection tubes. The top of each injection tube is equipped with a sealing iris assembly that cooperates with a syringe to assist in sealing and feeding. The injection tubes cooperate with the uniform feeding components. The exterior of the sealed incubator is equipped with a lifting assembly that drives the injection tubes to rise and fall.

[0008] Furthermore, the adjustable shaking assembly includes a servo geared motor fixed at the top center of the sealed incubator and a universal joint fixed at the top center of the electric rotary table. The output end of the servo geared motor is fixedly connected to an eccentrically positioned limiting shell. A miniature electric push rod is fixedly installed inside the eccentric limiting shell. A displacement block is fixedly connected to the end of the movable rod of the miniature electric push rod facing the axis. The side wall of the displacement block is slidably connected to the inner side wall of the eccentric limiting shell. A spherical connecting groove is opened at the bottom end of the displacement block. A threaded screw and a telescopic rod are fixedly connected sequentially from bottom to top at the top of the universal joint. A spherical joint is fixed at the top end of the telescopic rod. The spherical joint is engaged with the spherical connecting groove. A cross-shaped limiting groove is radially opened at the axis of the threaded screw. The lower side wall of the threaded screw is fixedly connected to the inner wall of the center of the culture dish support.

[0009] Through the above technical solution, the combination of a servo-driven geared motor-driven eccentric limiting shell and a micro electric push rod enables the culture dish to achieve a composite motion trajectory in the X / Y / Z axes, such as elliptical / spiral shaking. This accurately replicates the shear stress environment required for tissue engineering, allowing the culture dish to achieve multi-dimensional periodic shaking, simulating the mechanical stimulation of the in vivo microenvironment, promoting the adherence and growth of stem cells and the exchange of substances, and effectively enhancing cell activity and differentiation potential.

[0010] Furthermore, the culture dish limiting assembly includes a displacement frame, a cross-shaped limiting frame fixed to the central side wall of the displacement frame, the side wall of the cross-shaped limiting frame slidably connected to the inner side wall of the cross-shaped limiting groove, a plurality of mounting rings corresponding to the positions of the placement grooves on the displacement frame, a threaded cylinder rotatably connected to the top of the displacement frame, the inner side wall of the threaded cylinder being threadedly connected to the outer side wall of the threaded screw, a driven gear being sleeved and fixed to the outer side wall of the threaded cylinder, a servo motor and a protective shell being fixedly mounted on the upper surface of the displacement frame, a main gear being fixedly connected to the output end of the servo motor, the main gear and the driven gear meshing with each other, and the servo motor, main gear, driven gear and threaded cylinder are all located inside the protective shell.

[0011] Through the above technical solution, the ring array culture dish support frame, together with the culture dish limiting component, supports the simultaneous cultivation of multiple culture dishes. The cross limiting frame and the cross limiting groove limit each other. The gear transmission system of the threaded screw and the threaded cylinder, together with the uniform feeding component, can realize the rapid locking / releasing of the culture dishes, which greatly shortens the operation time.

[0012] Furthermore, the uniform feeding assembly is fixed in a circumferential array to the guide plates on the inner wall of the mounting ring, and to the diverting cones on the inner wall of the mounting ring. The outer surface of the diverting cones is provided with several dividing protrusions along the axis, and each dividing protrusion is located between two adjacent guide plates. The guide plates are radially provided with guide grooves on the side facing the center. There are flow ports between the edge of the diverting cones and the guide grooves. A fixing frame is fixed to the top of the diverting cones. A funnel is fixed to the inner side wall of the fixing frame. The bottom end of the funnel is located directly above the top of the diverting cones. The injection pipes are all located directly above the funnels. Through holes are provided on the lower side walls of the guide plates. A connecting frame is fixedly connected between the bottom ends of several guide plates. Several serrated grooves are provided on the upper surface of the connecting frame.

[0013] Through the above technical solution, the guide plate, the diversion cone, the funnel and the injection tube are linked together. The fluid is guided to be evenly distributed by the dividing protrusion and the guide groove. Combined with the connecting frame with the sawtooth groove design and the adjustable shaking component, the mixing effect is enhanced, ensuring that the culture reagent is evenly distributed in all corners of the culture dish, and further improving the mixing efficiency.

[0014] Furthermore, the lifting assembly includes connecting seats symmetrically fixed to the outer wall of the sealed incubator. Each connecting seat has an electric cylinder fixedly installed at its top. The length of the movable rod of the electric cylinder and the injection tube is not less than the height of the sealed incubator. A lifting plate is fixedly connected to the top of the electric cylinder. The surface of the lifting plate has several fixing holes that correspond to the position of the injection tube. The inner sidewall of each fixing hole is fixedly connected to the outer sidewall of the injection tube.

[0015] With the above technical solution, when the adjustable shaking component is running, the electric cylinder pushes the lifting plate up, which moves the injection tube to the top of the eccentric limiting shell to avoid obstructing the normal operation of the adjustable shaking component. The length of the moving rod of the electric cylinder and the injection tube is not less than the height of the sealed incubator, ensuring the injection stroke of the injection tube and allowing the injection tube to be close to the funnel to maintain the stability of the injection.

[0016] Furthermore, the sealing iris assembly includes a housing fixed to the top of the injection tube. A limiting groove is formed on the inner wall of the housing. An internal gear ring is rotatably connected to the inner wall of the limiting groove. Four evenly distributed transmission gears are meshed with the inner wall of the internal gear ring. All transmission gears are rotatably mounted on the bottom of the inner wall of the housing. Four slidingly fitted sealing wedges drive each of the transmission gears. The outer walls of each sealing wedge are provided with teeth that mesh with adjacent transmission gears. The four sealing wedges... When the blocks are combined, they cover the injection tube opening. The bottom end of each sealing wedge block is provided with a rectangular limiting protrusion. The bottom end of the inner wall of the housing is provided with a limiting groove II along the sliding trajectory of the sealing wedge block. The inner sidewall of the limiting groove II is slidably connected to the outer wall of the adjacent rectangular limiting protrusion. The top end of each housing is connected to a connecting pipe. The inner sidewall of the connecting pipe is provided with a threaded groove. The threaded groove is adapted to the connecting thread. The sidewall of the housing is provided with a movable opening. A locking mechanism for locking and limiting the inner gear ring is provided between the inner gear ring and the housing.

[0017] Through the above technical solution, the sealing iris assembly at the tip of the injection tube achieves zero leakage during syringe docking via a locking mechanism, preventing microbial intrusion. During reagent injection, the incubator remains sealed, preventing sample exposure to air and disruption of the overall culture environment. This ensures a safe culture environment while allowing for targeted reagent injection based on the specific culture needs of different samples, making it suitable for a wider range of environments.

[0018] Furthermore, the locking mechanism includes a limiting member fixed to the outer wall of the housing and a connecting member fixed to the outer wall of the inner gear ring. The connecting member and the limiting member are positioned correspondingly. A movable limiting plate is rotatably connected to the inner wall of the connecting member. The movable limiting plate is in a limiting fit with the limiting member. A magnet is fixed to the inner wall of the limiting member. The movable limiting plate is made of magnetically attractive metal material.

[0019] Through the above technical solution, when the sealing iris assembly is in a closed state, the magnet on the inner wall of the limiting component magnetically attracts the movable limiting plate, thereby locking and limiting the movable limiting plate, and thus completing the locking and limiting of the inner toothed ring of the sealing red iris assembly, ensuring the sealing stability of the sealing red iris assembly.

[0020] Furthermore, the sealed incubator has an opening on its side wall, and a sliding groove is provided on the inner side wall of the opening. A glass sealing door is slidably connected to the inner side wall of the sliding groove. A control panel and a placement rack are fixedly installed on the outer wall of the sealed incubator. The control panel is electrically connected to a servo reduction motor, a micro electric push rod, a servo motor, an electric rotary table, and an electric cylinder. The syringe is placed inside the placement rack.

[0021] Through the above technical solution, the glass-sealed door, in conjunction with the sliding track, allows for real-time observation of cell status without compromising airtightness, balancing operational convenience with contamination control. The control panel centrally manages the servo geared motor, micro electric push rod, servo motor, electric rotary table, and electric cylinder, and allows for programmable settings of parameters such as oscillation period, speed, and feed rate, achieving full-process automation without any manual intervention.

[0022] The beneficial effects of this invention are as follows:

[0023] With the adjustable shaking component and the culture dish limiting component, multiple samples can be simultaneously limited and locked. The combination of servo reduction motor driving eccentric limiting shell and micro electric push rod realizes the composite motion trajectory shaking of culture dish in three axes, accurately reproduces the shear stress environment required for tissue engineering, and enables culture dish to achieve multi-dimensional periodic shaking, simulates the mechanical stimulation of the in vivo microenvironment, promotes the adherence growth and material exchange of stem cells, and effectively enhances cell activity and differentiation potential.

[0024] (2) By setting up a uniform feeding component, the fluid of the injected reagent is guided to be evenly distributed. Combined with the sawtooth groove design of the connecting frame and the adjustable shaking component, the mixing effect is enhanced, ensuring that the reagent is evenly distributed in all corners of the petri dish, and further improving the mixing efficiency.

[0025] (3) By setting up a sealed iris assembly and a syringe, the sealed iris assembly and the syringe are connected in a sealed manner. During the injection of reagents, the incubator is kept sealed to avoid exposing the sample to the air and damaging the overall culture environment, thus ensuring the stability of the culture environment. Attached Figure Description

[0026] Figure 1 This is a perspective view of a stem cell culture device according to the present invention;

[0027] Figure 2 This is a partial structural diagram of a stem cell culture device according to the present invention;

[0028] Figure 3 This is a partial perspective view of a stem cell culture device according to the present invention;

[0029] Figure 4 This is a structural diagram of an adjustable shaking component of a stem cell culture device according to the present invention;

[0030] Figure 5 This is a structural diagram of the culture dish limiting component of a stem cell culture device according to the present invention;

[0031] Figure 6 This is a perspective view of the displacement frame of a stem cell culture device according to the present invention;

[0032] Figure 7This is a structural diagram of the uniform feeding component of a stem cell culture device according to the present invention;

[0033] Figure 8 This is a perspective view of the lifting component of a stem cell culture device according to the present invention;

[0034] Figure 9 This invention relates to a sealed iris assembly structure for a stem cell culture device. Figure 1 ;

[0035] Figure 10 This invention relates to a sealed iris assembly structure for a stem cell culture device. Figure 2 ;

[0036] Figure 11 This is a perspective view of a syringe in a stem cell culture device according to the present invention.

[0037] Reference numerals: 1. Sealed incubator; 2. Electric rotary table; 3. Adjustable shaking assembly; 4. Petri dish support rack; 5. Petri dish limiting assembly; 6. Uniform feeding assembly; 7. Lifting assembly; 8. Injection tube; 9. Sealed iris assembly; 10. Placement rack; 11. Control panel; 12. Syringe; 13. Glass sealing door; 14. Locking mechanism; 101. Incubator opening; 102. Slide groove; 301. Servo geared motor; 302. Eccentric limiting shell; 303. Miniature electric push rod; 304. Displacement block; 3041. Spherical connecting groove; 305. Telescopic rod; 306. Spherical joint; 307. Threaded screw; 3071. Cross limiting groove; 308. Universal joint; 401. Placement groove; 501. Displacement frame; 502. Mounting ring; 503. Cross limiting frame; 504. Threaded cylinder; 505. Servo motor 506. Main gear; 507. Driven gear; 508. Protective shell; 601. Guide plate; 6011. Guide groove; 6012. Through hole; 602. Diverter cone; 6021. Divider protrusion; 603. Flow port; 604. Fixing bracket; 605. Funnel; 606. Connecting bracket; 6061. Serrated groove; 701. Electric cylinder body; 702. Lifting plate; 703. Fixing hole; 704. Connection 901, housing; 9011, limiting groove one; 9012, limiting groove two; 9013, movable opening; 902, internal gear ring; 903, transmission gear; 904, sealing wedge block; 9041, tooth; 9042, rectangular limiting protrusion; 905, connecting pipe; 9051, threaded groove; 1201, connecting thread; 1401, limiting component; 1402, connecting component; 1403, movable limiting plate. Detailed Implementation

[0038] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0039] like Figures 1-11 As shown, a stem cell culture device in this embodiment includes a sealed culture box 1 and a syringe 12. The syringe 12 has a connecting thread 1201 on the side wall of the injection port. An electric rotating platform 2 is fixedly installed at the bottom of the sealed culture box 1. An adjustable shaking component 3 is provided above the electric rotating platform 2 to dynamically improve the efficiency of stem cell culture. The electric rotating platform 2 provides convenience for rotating and changing the position of the adjustable shaking component 3 for loading samples.

[0040] The adjustable shaking component 3 is equipped with a culture dish support frame 4 for positioning and supporting multiple culture dishes, and a culture dish limiting component 5 for pressing and limiting the culture dishes. Several placement slots 401 are fixed along a circular array on the upper surface of the culture dish support frame 4. Several uniform feeding components 6 for assisting feeding and mixing are provided on the culture dish limiting component 5. The uniform feeding components 6 are all corresponding to the positions of the placement slots 401. Several injection tubes 8 are slidably connected to the top of the sealed culture box 1. Each injection tube 8 is equipped with a sealing iris component 9 that cooperates with the syringe 12 to assist in sealing and feeding. The injection tubes 8 are all in cooperation with the uniform feeding components 6. The outside of the sealed culture box 1 is equipped with a lifting component 7 for driving the injection tubes 8 to rise and fall.

[0041] The adjustable rocking assembly 3 includes a servo geared motor 301 fixed at the top center of the sealed incubator 1 and a universal joint 308 fixed at the top center of the electric rotary table 2. The output end of the servo geared motor 301 is fixedly connected to an eccentrically positioned limiting shell 302. A miniature electric push rod 303 is fixedly installed inside the eccentric limiting shell 302. A displacement block 304 is fixedly connected to the end of the movable rod of the miniature electric push rod 303 facing the axis. The side wall of the displacement block 304 is slidably connected to the inner side wall of the eccentric limiting shell 302. A spherical connecting groove 3041 is provided at the bottom end of the displacement block 304. From bottom to top, a threaded screw 307 and a telescopic rod 305 are sequentially fixed to the top of the universal joint 308. The telescopic rod 305... A ball joint 306 is fixed at the top, and the ball joint 306 is engaged with the ball connecting groove 3041. A cross-shaped limiting groove 3071 is opened radially on the axis of the threaded screw 307. The lower side wall of the threaded screw 307 is fixedly connected to the inner wall of the center of the culture dish support frame 4. The combination of the eccentric limiting shell 302 and the micro electric push rod 303 driven by the servo reduction motor 301 realizes the composite motion trajectory of the culture dish in the X / Y / Z three axes (such as elliptical / spiral shaking), accurately reproduces the shear stress environment required for tissue engineering, and enables the culture dish to achieve multi-dimensional periodic shaking, simulate the mechanical stimulation of the in vivo microenvironment, promote the adherence and growth of stem cells and the exchange of substances, and effectively enhance cell activity and differentiation potential. The universal joint 308 and the ball joint 306 work together to allow the displacement block 304 to slide along the eccentric limiting shell 302 and shift at an angle. Combined with the length adjustment of the telescopic rod 305, this forms a "joint-like" flexible transmission system. This system adapts to the personalized oscillation needs of culture dishes of different diameters, enabling customized oscillation modes with different frequencies and amplitudes, thus meeting various stem cell culture requirements. In use, the servo reducer motor 301 drives the eccentric limiting shell 302 to rotate, and the micro-electric push rod 303 reciprocates to push the displacement block 304 laterally. Combined with the adaptive length adjustment of the telescopic rod 305 and the cooperation of the universal joint 308 and the ball joint 306, this causes the constantly tilting culture dish support frame 4 to sway circumferentially, achieving multi-dimensional periodic oscillation of the culture dish within the support frame 4. This increases the contact area between stem cells and oxygen and nutrients, significantly improving culture efficiency. Furthermore, when the culture dish needs to be stored or retrieved, the movable rod of the miniature electric push rod 303 is fully extended. At this time, the threaded screw 307 is located on the rotation axis of the output end of the servo reducer motor 301, adjusting the culture dish support frame 4 to a horizontal state, which facilitates the storage and retrieval of the culture dish on the placement slot 401.

[0042] The petri dish positioning assembly 5 includes a displacement frame 501, a cross-shaped positioning frame 503 fixedly connected to the central side wall of the displacement frame 501, the side wall of the cross-shaped positioning frame 503 slidably connected to the inner side wall of the cross-shaped positioning groove 3071, a plurality of mounting rings 502 corresponding to the positions of the placement groove 401 on the displacement frame 501, a threaded cylinder 504 rotatably connected to the top of the displacement frame 501, the inner side wall of the threaded cylinder 504 threadedly connected to the outer side wall of the threaded screw 307, a driven gear 507 sleeved and fixed on the outer side wall of the threaded cylinder 504, and a servo motor 505 and a protective device fixedly mounted on the upper surface of the displacement frame 501. The output end of the servo motor 505 is fixedly connected to the shell 508, and the main gear 506 meshes with the driven gear 507. The servo motor 505, the main gear 506, the driven gear 507, and the threaded cylinder 504 are all located inside the protective shell 508. The annular array-type culture dish support frame 4, together with the culture dish limiting component 5, supports the simultaneous cultivation of multiple culture dishes. The cross limiting frame 503 and the cross limiting groove 3071 limit each other. The gear transmission system of the threaded screw 307 and the threaded cylinder 504, together with the uniform feeding component 6, can realize the rapid locking / releasing of the culture dish, which greatly shortens the operation time. When the displacement frame 501 is raised or lowered, the servo motor 505 drives the main gear 506 to rotate. Through the meshing transmission of the slave gear 507, the threaded cylinder 504 is driven to rotate along the threaded screw 307. At the same time, the cross limit frame 503 slides along the cross limit groove 3071, thereby driving the displacement frame 501 to rise or fall. No manual operation is required, so that the device can be operated in a sealed environment, which makes it easier for staff to observe the state of stem cells.

[0043] The uniform feeding assembly 6 is fixed in a circumferential array to the guide plates 601 on the inner wall of the mounting ring 502, and to the diversion cones 602 fixed on the inner wall of the mounting ring 502. Several dividing protrusions 6021 are arranged along the axis on the outer surface of the diversion cones 602, with each protrusion located between two adjacent guide plates 601. A guide groove 6011 is radially opened on the side of the guide plate 601 facing the center. A flow inlet 603 separates the edge of the diversion cone 602 from the guide groove 6011. A fixing frame 604 is fixed to the top of the diversion cone 602, and a funnel 605 is fixed to the inner wall of the fixing frame 604. The bottom end of the funnel 605 is located at the top of the diversion cone 602. Directly above the tip, the injection tubes 8 are all located directly above the funnel 605. The lower sidewalls of the guide plates 601 are provided with through holes 6012. A connecting frame 606 is fixedly connected between the bottom ends of several guide plates 601. Several serrated grooves 6061 are provided on the upper surface of the connecting frame 606. The guide plates 601, the diverting cones 602, the funnel 605 and the injection tubes 8 are linked together. The fluid is guided to be evenly distributed by the separating protrusions 6021 and the guide grooves 6011. The connecting frame 606 designed with serrated grooves 6061 cooperates with the adjustable shaking component 3 to enhance the mixing effect and ensure that the culture reagent is evenly distributed in all corners of the culture dish, further improving the mixing efficiency. In use, when limiting the culture dish, the culture dish limiting component 5 drives the flow guide plate 601 to press against the bottom of the inner wall of the culture dish to complete the limiting; when it is necessary to inject culture reagent, the fluid in the injection tube 8 gathers at the tip of the flow splitting cone 602 through the funnel 605, and the flow splitting cone 602 and the slope of the dividing protrusion 6021 and the flow splitting cone 602 evenly distribute the fluid. The distributed fluid falls into the flow guide plate 601 through each flow port 603, and then the flow guide groove 6011 guides the fluid to each corner of the culture dish, evenly distributes and improves the mixing efficiency. When the culture dish is shaken, the serrated groove 6061 of the connecting frame 606 increases the shear force on the fluid, further improving the mixing quality. The through hole 6012 reduces the space occupied by the flow guide plate 601 in the culture dish.

[0044] The lifting assembly 7 includes connecting seats 704 symmetrically fixed to the outer wall of the sealed incubator 1. An electric cylinder 701 is fixedly installed at the top of each connecting seat 704. The length of the movable rod of the electric cylinder 701 and the injection tube 8 is not less than the height of the sealed incubator 1. A lifting plate 702 is fixedly connected to the top of the electric cylinder 701. Several fixing holes 703 corresponding to the position of the injection tube 8 are opened through the surface of the lifting plate 702. The inner side wall of the fixing hole 703 is fixedly connected to the outer side wall of the injection tube 8. When the adjustable shaking assembly 3 is running, the electric cylinder 701 pushes the lifting plate 702 to rise, driving the injection tube 8 to move above the eccentric limiting shell 302 to avoid obstructing the normal operation of the adjustable shaking assembly 3. The length of the movable rod of the electric cylinder 701 and the injection tube 8 is not less than the height of the sealed incubator 1 to ensure the injection stroke of the injection tube 8, so that the injection tube 8 can be close to the funnel 605 to maintain the stability of the injection.

[0045] The sealing iris assembly 9 includes a housing 901 fixed to the top of the injection tube 8. A limiting groove 9011 is formed on the inner wall of the housing 901. An internal gear ring 902 is rotatably connected to the inner wall of the limiting groove 9011. Four evenly distributed transmission gears 903 are meshed with the inner wall of the internal gear ring 902. Each transmission gear 903 is rotatably mounted on the bottom of the inner wall of the housing 901. Four slidingly fitted sealing wedges 904 drive each transmission gear 903. Teeth 9041 are formed on the outer walls of each sealing wedge 904, and these teeth mesh with adjacent transmission gears 903. When the four sealing wedges 904 are closed, they cover the opening of the injection tube 8. A rectangular limiting protrusion is provided at the bottom of each sealing wedge 904. Starting from 9042, the bottom of the inner wall of the housing 901 is provided with a limiting groove 9012 along the sliding trajectory of the sealing wedge block 904. The inner sidewall of the limiting groove 9012 is slidably connected to the outer wall of the adjacent rectangular limiting protrusion 9042. The top of the housing 901 is connected to a connecting pipe 905. The inner sidewall of the connecting pipe 905 is provided with a threaded groove 9051, which is threaded and adapted to the connecting thread 1201. The sidewall of the housing 901 is provided with a movable opening 9013. A locking mechanism 14 is provided between the inner gear ring 902 and the housing 901 to lock and limit the inner gear ring 902. The sealing iris assembly 9 at the top of the injection tube 8 achieves zero leakage when the syringe 12 is docked through the locking mechanism 14, preventing microbial intrusion. When the device is in the culture state, each sealing wedge 904 closes in a petal shape to seal the opening of the injection tube 8, ensuring the airtightness of the culture environment. When it is necessary to add culture components to a single culture dish, the connecting thread 1201 of the syringe 12 is threaded into the threaded groove 9051 of the connecting tube 905 to complete the sealing connection. Next, the internal gear ring 902 is rotated counterclockwise. Through the synchronous meshing of each transmission gear 903, the rectangular limiting protrusion 9042 slides along the limiting groove 9012, thereby driving the four sealing wedges 904 to move in tandem, exposing the opening of the injection tube 8. The syringe 12 can then inject in the sealed environment. After the injection is completed, the sealing iris assembly 9 is reset to ensure a seal. Finally, the syringe 12 is removed. During the injection process, the airtightness of the incubator is maintained to prevent the sample from being exposed to the air and disrupting the overall culture environment. While ensuring the sample culture environment, targeted reagent injection can be performed individually according to the culture requirements of different samples, making it applicable to a wider range of environments.

[0046] The locking mechanism 14 includes a limiting member 1401 fixed to the outer wall of the housing 901 and a connecting member 1402 fixed to the outer wall of the inner gear ring 902. The connecting member 1402 corresponds to the limiting member 1401. A movable limiting plate 1403 is rotatably connected to the inner wall of the connecting member 1402. The movable limiting plate 1403 is in a limiting engagement with the limiting member 1401. A magnet is fixed to the inner wall of the limiting member 1401. The movable limiting plate 1403 is made of a magnetically attractive metal material. When the sealing iris assembly 9 is closed... When in the closed state, rotate the movable limiting plate 1403 downwards until the movable limiting plate 1403 is inserted into the groove of the limiting member 1401. The magnet on its inner wall magnetically attracts the movable limiting plate 1403, thereby locking and limiting the movable limiting plate 1403, thus completing the locking and limiting of the inner toothed ring 902 of the sealing iris assembly 9, ensuring the sealing stability of the sealing iris assembly 9. When unlocking, it is only necessary to flip the movable limiting plate 1403 to disengage from the limiting member 1401. The structure is simple and reliable.

[0047] The sealed incubator 1 has an opening 101 on its side wall, and a groove 102 on the inner side wall of the opening 101. A glass sealing door 13 is slidably connected to the inner side wall of the groove 102. A control panel 11 and a placement rack 10 are fixedly installed on the outer wall of the sealed incubator 1. The control panel 11 is electrically connected to a servo reduction motor 301, a micro electric push rod 303, a servo motor 505, an electric rotary table 2, and an electric cylinder 701. The syringe 12 is placed inside the placement rack 10. The glass sealing door 13 cooperates with the groove 102, allowing real-time observation of cell status without compromising airtightness. This balances ease of operation with contamination control. When observing samples in different corners, the electric rotary table 2 can precisely drive the threaded screw 307 to rotate, thereby rotating the culture dish support rack 4. This allows for comprehensive observation of all samples from a single observation point. The control panel 11 centrally manages the servo geared motor 301, the miniature electric actuator 303, the servo motor 505, the electric rotary table 2, and the electric cylinder 701. It allows for programmable settings of parameters such as oscillation cycle, speed, and feed rate, achieving full automation without manual intervention. The syringe 12 is placed inside the rack 10 for convenient access by staff.

[0048] The working principle of this embodiment is as follows: When in use, slide open the glass sealing door 13, place the culture dish after injecting the sample on the placement groove 401 of the culture dish support rack 4, start the electric rotary table 2 through the control panel 11 to precisely drive the culture dish support rack 4 to rotate and change the placement position until it is completely filled. The servo motor 505 drives the main gear 506 to rotate. Through the meshing transmission of the slave gear 507, the threaded cylinder 504 is driven to rotate along the threaded screw 307. At the same time, the cross limit frame 503 slides along the cross limit groove 3071 to limit and slide, thereby driving the displacement frame 501 to descend, driving the uniform feeding component 6 to press and lock the culture dish. After that, the glass sealing door 13 can be reset.

[0049] During the cultivation process, the servo reduction motor 301 drives the eccentric limiting shell 302 to rotate, and the micro electric push rod 303 reciprocates to push the displacement block 304 to move laterally. With the adaptive adjustment of the length of the telescopic rod 305 and the cooperation of the universal joint 308 and the ball joint 306, the culture dish support frame 4, which is constantly changing and tilting, shakes along the circumference, realizing the multi-dimensional periodic shaking of the culture dish in the culture dish support frame 4. With the serrated groove 6061 of the connecting frame 606, the shear force on the fluid is increased, further improving the mixing quality, increasing the contact area between stem cells and oxygen and nutrients, and significantly improving the cultivation efficiency.

[0050] When reagents need to be added to the sample in a local culture dish, the movable rod of the miniature electric push rod 303 is fully extended. At this time, the threaded screw 307 is located on the rotation axis of the output end of the servo reduction motor 301, adjusting the culture dish support frame 4 to a horizontal state. The electric cylinder 701 pushes the lifting plate 702 to rise, driving the injection tube 8 to descend closer to the funnel 605, maintaining the stability of the injection. After locating the injection tube 8 corresponding to the position of the culture dish, the connecting thread 1201 of the syringe 12 is threadedly connected to the thread groove 9051 of the connecting tube 905 to complete the sealing connection. First, rotate the internal gear ring 902 counterclockwise. Through the synchronous meshing of each transmission gear 903, and with the rectangular limiting protrusion 9042 sliding along the limiting groove 9012, the four sealing wedge blocks 904 move in coordination, exposing the opening of the injection tube 8. The syringe 12 can then inject in a sealed environment. After the injection is completed, reset the sealing iris assembly 9 to ensure a seal. Finally, remove the syringe 12. During the injection process, always maintain the airtightness of the incubator to avoid exposing the sample to the air and disrupting the overall culture environment.

[0051] The fluid in the injection tube 8 is collected by the funnel 605 and falls on the tip of the diversion cone 602. The fluid is evenly distributed by the separation protrusion 6021 and the slope of the diversion cone 602. The diverted fluid falls into the guide plate 601 through each flow port 603. Then, the guide groove 6011 guides the fluid to each corner of the culture dish, evenly distributing it to improve mixing efficiency. Finally, the adjustable shaking component 3 is started again to mix the sample.

[0052] The above description is merely a preferred embodiment of the present invention and is not intended to limit the scope of protection of the present invention.

Claims

1. A stem cell culture device, comprising a sealed culture chamber (1) and a syringe (12), characterized in that: The syringe (12) has a connecting thread (1201) on the side wall of the injection port. An electric rotating table (2) is fixedly installed at the bottom of the sealed culture box (1). An adjustable shaking component (3) is provided above the electric rotating table (2) to dynamically improve the efficiency of stem cell culture. The adjustable shaking component (3) is provided with a culture dish support frame (4) for positioning and supporting multiple culture dishes, and a culture dish limiting component (5) for pressing and limiting the culture dishes. The upper surface of the culture dish support frame (4) is fixed with several placement slots (401) along a circular array. The culture dish limiting component (5) is provided with several uniform feeding components (6) for assisting feeding and mixing. The uniform feeding components (6) are all corresponding to the positions of the placement slots (401). The top of the sealed culture box (1) is sealed and slidably connected with several injection tubes (8). The top of each injection tube (8) is provided with a sealing iris component (9) that cooperates with the syringe (12) to assist in sealing and feeding. The injection tubes (8) are all in cooperation with the uniform feeding components (6). The outside of the sealed culture box (1) is provided with a lifting component (7) for driving the injection tubes (8) to rise and fall. The adjustable rocking assembly (3) includes a servo geared motor (301) fixed at the top center of the sealed incubator (1) and a universal joint (308) fixed at the top center of the electric rotary table (2). The output end of the servo geared motor (301) is fixedly connected to an eccentrically positioned limiting shell (302) with a laterally eccentric arrangement. A miniature electric push rod (303) is fixedly installed inside the eccentric limiting shell (302). A displacement block (304) is fixedly connected to the end of the movable rod of the miniature electric push rod (303) facing the axis. The side wall of the displacement block (304) is connected to the eccentric limiting shell (302). The inner sidewall is slidably connected, and the bottom end of the displacement block (304) is provided with a spherical connecting groove (3041). The top end of the universal joint (308) is fixed with a threaded screw (307) and a telescopic rod (305) from bottom to top. The top end of the telescopic rod (305) is fixed with a spherical joint (306). The spherical joint (306) is engaged with the spherical connecting groove (3041). The axis of the threaded screw (307) is radially provided with a cross-shaped limiting groove (3071). The lower sidewall of the threaded screw (307) is fixedly connected to the inner wall of the center of the culture dish support frame (4). The petri dish positioning assembly (5) includes a displacement frame (501), a cross-shaped positioning frame (503) is fixedly connected to the central side wall of the displacement frame (501), the side wall of the cross-shaped positioning frame (503) is slidably connected to the inner side wall of the cross-shaped positioning groove (3071), the displacement frame (501) is provided with a plurality of mounting rings (502) corresponding to the positions of the placement groove (401), and a threaded cylinder (504) is rotatably connected to the top end of the displacement frame (501), the inner side wall of the threaded cylinder (504) is connected to the threaded screw (307). The outer wall of the screw cylinder (504) is threaded, and the outer wall of the screw cylinder (504) is fitted with a driven gear (507). The upper surface of the displacement frame (501) is fixedly mounted with a servo motor (505) and a protective shell (508). The output end of the servo motor (505) is fixedly connected with a main gear (506). The main gear (506) and the driven gear (507) mesh with each other. The servo motor (505), the main gear (506), the driven gear (507), and the screw cylinder (504) are all located inside the protective shell (508). The uniform feeding assembly (6) is fixed in a circumferential array to the guide plates (601) on the inner wall of the mounting ring (502) and to the diverting cones (602) on the inner wall of the mounting ring (502). The outer surface of the diverting cones (602) is provided with a plurality of dividing protrusions (6021) arranged along the axis. The dividing protrusions (6021) are all located between two adjacent guide plates (601). The guide plates (601) are radially provided with guide grooves (6011) on the side facing the center. The edge of the diverting cones (602) and the guide grooves (6011) are separated by flow ports (6). 03), the top of the diversion cone (602) is fixed with a fixing frame (604), the inner side wall of the fixing frame (604) is fixed with a funnel (605), the bottom end of the funnel (605) is located directly above the top of the diversion cone (602), the injection pipe (8) is located directly above the funnel (605), the lower side wall of the guide plate (601) is provided with through holes (6012), and a connecting frame (606) is fixedly connected between the bottom ends of several guide plates (601), and several sawtooth grooves (6061) are provided on the upper surface of the connecting frame (606).

2. The stem cell culture device according to claim 1, characterized in that, The lifting assembly (7) includes a connecting seat (704) symmetrically fixed to the outer wall of the sealed incubator (1). An electric cylinder (701) is fixedly installed at the top of each connecting seat (704). The length of the movable rod of the electric cylinder (701) and the injection pipe (8) is not less than the height of the sealed incubator (1). A lifting plate (702) is fixedly connected to the top of the electric cylinder (701). Several fixing holes (703) corresponding to the position of the injection pipe (8) are opened through the surface of the lifting plate (702). The inner sidewall of the fixing hole (703) is fixedly connected to the outer sidewall of the injection pipe (8).

3. The stem cell culture device according to claim 1, characterized in that, The sealing iris assembly (9) includes a housing (901) fixed to the top of the injection tube (8). A limiting groove (9011) is formed on the inner wall of the housing (901). An internal gear ring (902) is rotatably connected to the inner wall of the limiting groove (9011). Four evenly distributed transmission gears (903) are meshed with the inner wall of the internal gear ring (902). Each transmission gear (903) is rotatably mounted on the bottom of the inner wall of the housing (901). Four slidingly fitted sealing wedges (904) drive each transmission gear (903). Teeth (9041) are formed on the outer wall of each sealing wedge (904), and these teeth (9041) mesh with adjacent transmission gears (903). When the four sealing wedges (904) are joined together, they cover the injection tube. 8) The bottom end of the sealing wedge block (904) is provided with a rectangular limiting protrusion (9042). The bottom end of the inner wall of the housing (901) is provided with a limiting groove (9012) along the sliding trajectory of the sealing wedge block (904). The inner side wall of the limiting groove (9012) is slidably connected to the outer wall of the adjacent rectangular limiting protrusion (9042). The top end of the housing (901) is connected to a connecting pipe (905). The inner side wall of the connecting pipe (905) is provided with a threaded groove (9051). The threaded groove (9051) is threaded and adapted to the connecting thread (1201). The side wall of the housing (901) is provided with a movable opening (9013). A locking mechanism (14) for locking and limiting the inner gear ring (902) is provided between the inner gear ring (902) and the housing (901).

4. The stem cell culture device according to claim 3, characterized in that, The locking mechanism (14) includes a limiting member (1401) fixed to the outer wall of the housing (901) and a connecting member (1402) fixed to the outer wall of the inner gear ring (902). The connecting member (1402) is positioned corresponding to the limiting member (1401). A movable limiting plate (1403) is rotatably connected to the inner wall of the connecting member (1402). The movable limiting plate (1403) is in a limiting fit with the limiting member (1401). A magnet is fixed to the inner wall of the limiting member (1401). The movable limiting plate (1403) is made of magnetically attracted metal material.

5. A stem cell culture device according to claim 1, characterized in that: The sealed incubator (1) has an opening (101) on its side wall. The inner side wall of the opening (101) has a groove (102). The inner side wall of the groove (102) is slidably connected to a glass sealing door (13). The outer wall of the sealed incubator (1) is fixedly installed with a control panel (11) and a placement rack (10). The control panel (11) is electrically connected to a servo reduction motor (301), a micro electric push rod (303), a servo motor (505), an electric rotary table (2), and an electric cylinder (701). The syringe (12) is placed inside the placement rack (10).

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